CN116195591B - Bactericide composition for preventing and treating gummy stem blight of melons - Google Patents

Bactericide composition for preventing and treating gummy stem blight of melons Download PDF

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Publication number
CN116195591B
CN116195591B CN202310074175.6A CN202310074175A CN116195591B CN 116195591 B CN116195591 B CN 116195591B CN 202310074175 A CN202310074175 A CN 202310074175A CN 116195591 B CN116195591 B CN 116195591B
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stem blight
gummy stem
thymol
preventing
treating
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CN116195591A (en
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秦健
吴永官
程亮
黎炎
莫阳朋
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Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences
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Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/28Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
    • A01N47/38Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/08Oxygen or sulfur directly attached to an aromatic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/80Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00Fungicides
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to the technical field of pesticides, in particular to a bactericide composition for preventing and treating gummy stem blight of melons. A bactericide composition for preventing and treating melon gummy stem blight comprises thymol and prochloraz or fluorothiazole pyrithione as effective components. The activity of the bactericide composition for preventing and treating the gummy stem blight of the melons is not simple superposition of the activities of the components, has obvious synergistic effect, can improve the effect of preventing and treating the gummy stem blight of the melons, reduces the application dosage of pesticides and reduces the prevention and treatment cost of the gummy stem blight of the melons.

Description

Bactericide composition for preventing and treating gummy stem blight of melons
Technical Field
The invention relates to the technical field of pesticides, in particular to a bactericide composition for preventing and treating gummy stem blight of melons.
Background
Melon gummy stem blight (Stagonosporopsis cucurbitacearum) can damage the leaves, stems and fruits of melons. Brown leaf spots are formed when the leaves are damaged, and the leaves are quickly dried up when serious, which is called leaf blight; the damage to the tendrils can cause gummosis and ulceration of the tendrils, and the tendrils die when serious; the fruit may cause black rot of the pulp. When the disease is serious, the death rate of plants is up to 60-80%, and the yield is reduced by more than 30%, so that the yield and quality of melons are affected, and the healthy development of melon industry is restricted. Chemical agents are the most common method for preventing and treating melon gummy stem blight, but long-term large-dose application of single chemical agents causes a plurality of problems such as drug resistance, pesticide residues, environmental pollution and the like.
Thymol is originally obtained by separating from herba Agrimoniae, has a chemical name of 3-methyl-6-isopropyl phenol, also known as thymol, thymol and the like, is an isomer of carvacrol, has a chemical formula of C 10H14 O, has a molecular weight of 150.22, is a single-phenol plant essential oil, has biological activities of antibiosis, antioxidation, insect expelling, anticancer and the like, and is widely used for spice manufacture, preservative, medical bactericide, insect repellent, antioxidant and the like at present. Thymol is easy to obtain, stable in chemical property, low in toxicity and low in residue, is easy to degrade in the environment, meets the development requirement of biological pesticides, and has wide application prospect as the development of the biological pesticides. The results of indoor bioassay and field efficacy measurement show that: thymol has good control effects on fusarium wilt, anthracnose, theca fulgorica, banded sclerotial blight, gray mold and the like.
In the course of agricultural pest control, two or more active ingredients are usually compounded in order to slow down the occurrence of drug resistance. The inventor discovers that after being compounded with prochloraz or fluorothiazole pyrithione, the thymol has remarkable synergism on melon gummy stem blight through a large amount of long-term experiments.
At present, the prior art has no report on the compounding of thymol and prochloraz or fluorothiazole pyrithione.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art.
Disclosure of Invention
The invention aims to provide a bactericide composition for preventing and treating melon gummy stem blight, which solves the problem of long-term large-dose application of a single chemical agent.
In order to achieve the above purpose, the present invention provides the following technical solutions:
A bactericide composition for preventing and treating melon gummy stem blight comprises thymol and prochloraz or fluorothiazole pyrithione as effective components.
Preferably, the mass ratio of thymol to prochloraz is 1-20:10-1.
Preferably, the mass ratio of thymol to prochloraz is 1:1.
Preferably, the mass ratio of thymol to oxazopraziclomefone is 1-15:30-1.
Compared with the prior art, the invention has the following beneficial effects:
(1) The thymol disclosed by the invention influences the metabolism and energy metabolism of microorganisms mainly by destroying the cell structure of pathogenic bacteria and damaging genetic material DNA; prochloraz affects the biosynthesis of sterols, so that the cell function of the thalli is destroyed, and the antibacterial effect is achieved; fluobuprofezin achieves a bactericidal effect by inhibiting the binding protein of cholesterol. When the thymol and prochloraz or fluorothiazole pyrifos with different action mechanisms are compounded into the binary pesticide composition, the generation of drug resistance can be delayed, and the defect of single application of any one of the active ingredients can be effectively overcome.
(2) The indoor bioassay test shows that the activity of the pesticide composition for preventing and treating the gummy stem blight of the melons is not simple superposition of the activities of the components, has obvious synergistic effect, can improve the effect of preventing and treating the gummy stem blight of the melons, reduces the application dosage of the pesticide and reduces the prevention and treatment cost of the gummy stem blight of the melons.
Detailed Description
The following description of the embodiments of the present invention will be apparent from the description of the embodiments of the present invention, which is provided in part, but not in whole. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, are intended to fall within the scope of the present invention.
Examples: indoor biological activity test of thymol after thymol is compounded
1. Test agent: 98% thymol active ingredient (Shanghai Michelin Biotechnology Co., ltd.), 98% prochloraz active ingredient (Shenyang scientific chemical Co., ltd.), 95% fluorothiazolyl pyrithione (Kedi Hua agricultural technology Co., ltd.);
2. Test strain: gummy stem blight bacteria (Stagonosporopsis cucurbitacearum);
3. test method (reference NY/T1156.2-2006, section 2 of pesticide laboratory bioassay test guidelines, test Petri dish method for inhibiting growth of pathogenic fungi hyphae)
3.1 Preparation of strains: taking out the gummy stem blight bacteria from the refrigerator at the temperature of 4 ℃, inoculating the gummy stem blight bacteria on a PDA culture medium, and culturing the gummy stem blight bacteria in a constant temperature incubator at the temperature of 28 ℃ for 7 days for later use;
3.2 preparing a medicament: firstly, dissolving a raw material with dimethyl sulfoxide to prepare a single-dose mother solution, diluting with sterilized water, setting a plurality of groups of proportions, and setting 6 mass concentration gradients of each single-dose mother solution and the proportion mixture according to an equal proportion method;
3.3 inoculation and drug treatment: mixing 1mL of the liquid medicine with 9mL of PDA culture medium, pouring into a culture dish with the diameter of 9cm, and cooling to prepare a culture medium plate containing liquid medicine with different concentrations; a7 mm diameter plaque was cut from the edge of the colony to be tested using a punch and inoculated into the center of the PDA plate containing the series of concentration agents. Treatments without any drug solution were set as blank, each treatment was repeated 5 times, and all treatments were incubated at 28℃in a constant temperature incubator.
3.4 Recording and calculating: when the diameter of the bacterial colony obtained by the blank control treatment is 2/3 of the diameter of the culture dish, measuring the diameter of each bacterial colony by using a crisscross method, and calculating the inhibition rate of each treatment agent on the growth of hyphae. The hypha growth inhibition rate was converted into a several-value (y), the concentration (mg/L) of the drug was converted into an logarithmic value (x) based on 10, the virulence regression equation of each test drug was calculated by DPS software, the concentration (EC 50) and the correlation coefficient in the effective inhibition were calculated, and the co-toxicity coefficient (CTC value) of the mixture was calculated according to Sun Yunpei method.
Hypha growth inhibition ratio = [ (control colony diameter-cake diameter) - (treated colony diameter-cake diameter) ]/(control colony diameter-cake diameter) ×100%;
Measured virulence index (ATI) = (standard agent EC 50 ≡test agent EC 50) ×100;
theoretical Toxicity Index (TTI) =a agent toxicity index x percentage of agent toxicity index in mixture + B agent toxicity index x percentage of agent toxicity in mixture;
Co-toxicity coefficient (CTC) = [ actual drug susceptibility index (ATI)/(theoretical drug susceptibility index (TTI) ] x 100.
5. Evaluation of drug efficacy
Dividing the standard according to the joint action: the co-toxicity coefficient (CTC) is more than or equal to 120, and the synergistic effect is shown; the cotoxicity coefficient (CTC) is less than or equal to 80 and shows antagonism; 80< co-toxicity coefficient (CTC) <120 shows additive effect. The experimental results are shown in tables 1-2.
TABLE 1 determination of indoor biological Activity of thymol and prochloraz in combination with Ralstonia solani
Medicament name and ratio EC50(mg/L) ATI TTI CTC
Thymol 98.9713 100.0000 -- --
Prochloraz 3.4285 2886.7231 -- --
Thymol 1: prochloraz 10 2.8236 3505.1459 2633.3846 133.1042
Thymol 1: prochloraz 5 1.7958 5511.2652 2422.2692 227.5249
Thymol 1: prochloraz 3 1.1243 8802.9263 2190.0423 401.9523
Thymol 1: prochloraz 1 0.8406 11773.8877 1493.3615 788.4151
Thymol 3: prochloraz 1 4.0025 2472.7370 796.6808 310.3799
Thymol 5: prochloraz 1 13.5235 731.8468 564.4538 129.6557
Thymol 10: prochloraz 1 16.0305 617.3937 353.3385 174.7315
Thymol 20: prochloraz 1 28.5730 346.3805 232.7011 148.8521
As can be seen from Table 1, the mass ratio of thymol to prochloraz is 1-10:15-1, the co-toxicity coefficient for inhibiting the growth of the mycelium of the gummy stem blight bacteria (Stagonosporopsis cucurbitacearum) is more than 120, namely the biological activity of thymol and prochloraz on the gummy stem blight disease after being compounded shows a synergistic effect, especially when the mass ratio is 1: when 1, the co-toxicity coefficient reaches 788.4151, and the synergy is most obvious.
Table 2 determination of indoor biological Activity of thymol and Fluothiapiprolin in combination with Ralstonia cucumeris
As can be seen from table 2, the mass ratio of thymol to oxazopraziclomefone is 1-15: within 30-1, the co-toxicity coefficient for inhibiting the growth of the mycelium of the gummy stem blight of melon (Stagonosporopsis cucurbitacearum) is more than 120, namely the biological activity of the thymol and the fluorothiazole pyrithione after being compounded shows a synergistic effect on the gummy stem blight of melon.
In conclusion, when the bactericide thymol is compounded with prochloraz or fluorothiazole pyrithione according to a certain proportion, the bactericidal agent has a synergistic effect on the biological activity of the melon gummy stem blight.
The foregoing descriptions of specific exemplary embodiments of the present invention are presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application to thereby enable one skilled in the art to make and utilize the invention in various exemplary embodiments and with various modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.

Claims (2)

1. The bactericide composition for preventing and treating the melon gummy stem blight is characterized in that the active ingredients of the bactericide composition are compounded by thymol and prochloraz, and the mass ratio of the thymol to the prochloraz is 1-20:10-1.
2. The bactericide composition for preventing and treating gummy stem blight according to claim 1, wherein the mass ratio of thymol to prochloraz is 1:1.
CN202310074175.6A 2023-02-07 2023-02-07 Bactericide composition for preventing and treating gummy stem blight of melons Active CN116195591B (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101888843A (en) * 2006-07-27 2010-11-17 杜邦公司 Fungicidal azocyclic amides
CN101969781B (en) * 2007-10-23 2015-01-28 杜邦公司 Fungicidal compounds and mixtures
EP2910126A1 (en) * 2015-05-05 2015-08-26 Bayer CropScience AG Active compound combinations having insecticidal properties
CN115281213A (en) * 2022-08-18 2022-11-04 广西壮族自治区农业科学院 Sugarcane seed stem coating agent applied at normal temperature in large scale and application thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011051243A1 (en) * 2009-10-29 2011-05-05 Bayer Cropscience Ag Active compound combinations

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101888843A (en) * 2006-07-27 2010-11-17 杜邦公司 Fungicidal azocyclic amides
CN101969781B (en) * 2007-10-23 2015-01-28 杜邦公司 Fungicidal compounds and mixtures
EP2910126A1 (en) * 2015-05-05 2015-08-26 Bayer CropScience AG Active compound combinations having insecticidal properties
CN115281213A (en) * 2022-08-18 2022-11-04 广西壮族自治区农业科学院 Sugarcane seed stem coating agent applied at normal temperature in large scale and application thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
2016年登记或上市的农药品种;吴峤;杨吉春;刘彦斐;;农药(第01期);全文 *

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