CN104705324A - Sterilizing composition containing zhongshengmycin and chitosan oligosaccharide - Google Patents
Sterilizing composition containing zhongshengmycin and chitosan oligosaccharide Download PDFInfo
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- CN104705324A CN104705324A CN201510111071.3A CN201510111071A CN104705324A CN 104705324 A CN104705324 A CN 104705324A CN 201510111071 A CN201510111071 A CN 201510111071A CN 104705324 A CN104705324 A CN 104705324A
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- zineb
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Abstract
The invention discloses a sterilizing composition containing zhongshengmycin and chitosan oligosaccharide. The sterilizing composition comprises the following effective constituents: zineb, zhongshengmycin and chitosan oligosaccharide, wherein the mass fraction ratio of zineb to zhongshengmycin to chitosan oligosaccharide is (10-20): (2-3): (1-3). According to the sterilizing composition disclosed by the invention, the functional mechanisms of zineb, zhongshengmycin and chitosan oligosaccharide are different, and can be mixed and blended within a reasonable proportional range to play the synergetic effects, so as to greatly improve the pesticide effect, reduce the usage amount of the pesticide and the usage cost, meanwhile expand the bactericidal spectrum and facilitate delaying production of bacterial resistance to the pesticide.
Description
Technical field
The present invention relates to a kind of pesticide synergistic composition, specifically containing the pesticide synergistic composition of Zhongshengmycin, chitosan oligosaccharide and zineb.
Background technology
Bacterial disease is the disease caused by bacterium infection process, as soft rot, canker, bacterial wilt etc.Bacterial disease is the important disease affecting China's agricultural production, and bacterial disease is once outburst, and often for want of the agricultural chemicals of high-efficiency low-toxicity prevents and prevents not in time, causes the vegetables underproduction, causes larger economic loss.Citrus bacterial canker disease is a kind of bacterial disease, mainly the cause harm tender tip, tender leaf and young fruit.Affect photosynthesis and fruit appearance quality time slight, cause a large amount of fallen leaves and shedding time serious, having a strong impact on yield and quality, is domestic and international important quarantine object.In recent years, increasing of the Citrus Cultivars of planting along with Guangxi, the generation of canker is also more and more general, and citrus bacterial canker disease prophylactico-therapeutic measures has a lot, and chemical control is important measures.In the medicament of existing chemical control, copper agent control efficiency is better, but easily brings out mite evil and easily produce poisoning, affects fruit quality.Therefore, seek efficient, low toxicity, safety citrus canker preventing and treating medicine and seem particularly important.
Zhongshengmycin, chemical name is: 1-N glucoside base streptolidine base-2-amino 1B-2 deoxidation gulose amine is N-glucoside antibiotic, its has a broad antifungal spectrum, can resisting gram-positive, negative germ, mycobacterium, saccharomycete and filamentous fungi.To crops pathogenic bacteria and dish Bacteria erwinia, avenae subsp.citrull, Xanthomonas oryzae, citrus bacterial canker disease, fusarium graminearum etc., all there is obvious antibacterial activity especially.But the long-time single continuous use of antibiotics agricultural chemicals, the pesticide resistance of germ will strengthen, and causes Pesticide use amount to increase, and control efficiency declines.
Zineb, chemical name is: zinc ethylenebisdithiocarbamate, is the protectant that a kind of foliage spray uses, has stronger action of contace poison, and also have good control action to various bacterial disease to many germs, late disease bacteria and anthrax bacteria.Zineb is to plant safety, chemical property is more active, different thiocyanation compound is easily oxidized in water, enzyme cause of disease thalline being included to-SH base has strong inhibitory action, and can directly kill germ spore, suppress the germination of spore, stop in germ invaded plants body, but very little to the mycelial lethal effect of the cause of disease in invaded plants body.
Chitosan oligosaccharide, also claims amino-oligosaccharide, is take marine organisms as raw material, adopts biotechnology to develop, and chitosan oligosaccharide, by being adsorbed on bacterial cell surface, forming one deck polymeric membrane, thus plays restraining and sterilizing bacteria effect; Can penetrate in bacterial cell body, the cytoplasm with anion in cyton be flocculated, upsets the normal physiological activity of cell, thus kill bacteria.Chitosan oligosaccharide can activate plant cell simultaneously, regulates and Promoting plant growth, improves resistance against disadvantage, strengthens crop cold resistance, drought resisting, waterlogging-resistant ability, removes disease, reaches increase yield, improves the object of quality.The control that chitosan oligosaccharide is used for bacteriosis has been reported, and as " Study On Amino-oligosaccharide Against Ralstonia Solanacearum " that Agricultural University Of Southwest delivers, show on NA flat board, chitosan oligosaccharide can suppress the growth of bacterial wilt of tomato completely at 400 times and following concentration.
Different pesticide active ingredients is combined, synergistic effect is significantly filled a prescription and greatly can be improved preventive effect, reduce dosage, the pollution reducing use cost and reduce environment, can also delay pathogen simultaneously and produce resistance, be solve the quick and effective approach of one that current single use agricultural chemicals produces resistance and drug cost high problem.
At present, also there is no Zhongshengmycin, zineb and chitosan oligosaccharide mixture, for preventing and treating the relevant report of citrus bacterial canker disease.
Summary of the invention
Technical problem to be solved by this invention is, a kind of bactericidal composition being active ingredient with Zhongshengmycin, zineb and chitosan oligosaccharide is provided, this bactericidal composition is used for the control of citrus bacterial canker disease, effectively can delay the resistance of pathogen and reduce the pollution of Pesticide use cost and minimizing environment.
The present invention is achieved through the following technical solutions:
Contain a bactericidal composition for Zhongshengmycin and chitosan oligosaccharide, active ingredient is made up of zineb, Zhongshengmycin and chitosan oligosaccharide.
The described bactericidal composition containing Zhongshengmycin and chitosan oligosaccharide, the ratio of quality and the number of copies of zineb, Zhongshengmycin and chitosan oligosaccharide is 10-20:2-3:1-3, and total active ingredient accounts for 30% ~ 60% of gross weight.
The described bactericidal composition containing Zhongshengmycin and chitosan oligosaccharide, according to method known in those skilled in the art, adds respective secondary auxiliary agent and is prepared into wetting powder.
The adjuvant that bactericidal composition of the present invention uses comprises the auxiliary elements such as filler, dispersant, wetting agent, diffusant, disintegrant, stabilizing agent or other is of value to the material that active ingredient is stablized in the formulation and drug effect plays, and is all the various compositions commonly using in pesticidal preparations or allow to use.
Bactericidal composition of the present invention is mainly used in preventing and treating the canker on mandarin tree.
The present invention has the following advantages and significant technique effect: the mechanism of action of zineb, Zhongshengmycin and chitosan oligosaccharide is different, in rational proportion, mixture has synergistic function, significantly can improve drug effect, reduce Pesticide use amount and drug cost, expand fungicidal spectrum simultaneously, be conducive to delaying the drug-fast generation of germ.
Embodiment
Be described further technical scheme of the present invention and technique effect below in conjunction with specific embodiment, percentage of the present invention is all weight percentage.
The embodiment of the present invention is the method adopting Toxicity Determination and field trial to combine.
Assays Example 1: the composite toxicity test to citrus processing of zineb, Zhongshengmycin and chitosan oligosaccharide
Subjects: citrus processing (Xanthomonas citri (Hahse)) (pick up from field, and through separation and purification)
Test method: test is with reference to " pesticide bioassay technology " (old spring chief editor, publishing house of Beijing Agricultural University publishes), and People's Republic of China (PRC) agricultural industry criteria NY/T1156.16-2008 " farm-chemical indoor determination test rule bactericide the 16th part: bacteria growing inhibiting amount test turbidity method ", adopt turbidity method to measure zineb, Zhongshengmycin and chitosan oligosaccharide to the indoor virulence of citrus processing.
The cultivation of A, non-target bacteria: (1LNA medium is containing polyprotein peptone 5g to select NA medium (nutrient agar) medium, dusty yeast 1g, beef extract 3g, sucrose 15g, agar 17g, pH7.0 is adjusted to the NaOH of 10mol/L) passivation cultivation citrus processing 48h in 30 DEG C of bacteriological incubators, picking list bacterium colony is in 30ml NA liquid nutrient medium, and under being placed in 28 DEG C of-30 DEG C of conditions, concussion cultivates (120r/min) 18-30h to target bacteria growing to exponential phase.
B, medicament zineb, Zhongshengmycin and chitosan oligosaccharide process and cultivation: the former medicine of zineb is first mixed with 1% zineb mother liquor with acetone solution, then dilute with the Tween-80 aqueous solution of 0.1%; The female medicine of Zhongshengmycin is directly dissolved as 100 μ g/ml mother liquors with distilled water, 5 ~ 7 series mass concentration gradients are set, the female medicine of chitosan oligosaccharide is directly dissolved as 120 μ g/ml mother liquors with distilled water, 5 ~ 7 series mass concentration gradients are set, medicament is added in 30ml NA liquid nutrient medium by the concentration quantitative of design, often process arranges 4 repetitions, and establishes the process not containing active ingredient not make blank (CK).The non-target bacteria 100ul bacterium liquid of exponential phase is inoculated respectively in each process medium, be placed in concussion under 28 DEG C of-30 DEG C of conditions and cultivate (120r/min) 16-20h when reaching exponential phase to the contrast bacteria growing of non-adding medicine, measure and record the turbidity of each process.
The mensuration of turbidity: the bacterium before measuring chemicals treatment and after process is at the absorbance value of 600nm.
Computational methods:
Carry out statistical analysis with DPS data processing software, calculate the EC of each medicament
50, control efficiency is converted into probability value (y), and liquor strength (ug/ml) converts logarithm value (x) to, calculates virulence equation and concentration EC in suppressing with method of least squares
50, then press toxicity index and co-toxicity coefficient (CTC) that the abundant method of Sun Yun calculates medicament.Toxicity test the results are shown in Table 1.
Actual measurement toxicity index (ATI)=(standard agent EC
50/ reagent agent EC
50) × 100
In theoretical toxicity index (TTI)=A medicament toxicity index × mixture A percentage composition+B medicament toxicity index × mixture in the percentage composition of B
Co-toxicity coefficient (CTC)=[the theoretical toxicity index (TTI) of mixture actual measurement toxicity index (ATI)/mixture] × 100
When CTC≤80, composition shows as antagonism, works as 80<CTC<120, and composition shows as summation action, and when CTC >=120, composition shows as synergistic effect.
Table 1 zineb, Zhongshengmycin and chitosan oligosaccharide and the composite Toxicity Determination result to citrus processing thereof
| Reagent agent | Proportioning | EC 50(μg/mL) | ATI | TTI | Co-toxicity coefficient CTC |
| Zineb | / | 19.06 | 136.26 | / | / |
| Zhongshengmycin | / | 2.49 | 1042.27 | / | / |
| Chitosan oligosaccharide | / | 25.97 | 100.00 | / | / |
| Zineb: Zhongshengmycin: chitosan oligosaccharide | 10:2:1 | 6.91 | 375.95 | 272.86 | 137.8 |
| Zineb: Zhongshengmycin: chitosan oligosaccharide | 10:2:3 | 6.77 | 383.35 | 249.81 | 153.5 |
| Zineb: Zhongshengmycin: chitosan oligosaccharide | 10:3:2 | 5.82 | 446.51 | 312.63 | 142.8 |
| Zineb: Zhongshengmycin: chitosan oligosaccharide | 20:3:3 | 6.13 | 423.94 | 236.62 | 179.2 |
| Zineb: Zhongshengmycin: chitosan oligosaccharide | 20:3:1 | 8.21 | 316.42 | 248.00 | 127.6 |
| Zineb: Zhongshengmycin: chitosan oligosaccharide | 20:2:1 | 7.60 | 341.89 | 213.47 | 160.2 |
| Zineb: Zhongshengmycin: chitosan oligosaccharide | 20:2:3 | 8.17 | 317.70 | 204.39 | 155.4 |
In table 1, result shows, zineb, Zhongshengmycin, chitosan oligosaccharide are to the EC of citrus bacterial canker disease
50be respectively 19.06mg/L, 2.49mg/L and 25.97mg/L, when zineb, Zhongshengmycin and chitosan oligosaccharide are in 10-20:2-3:1-3 scope mixture, co-toxicity coefficient (CTC) is all greater than 120, shows obvious synergistic effect.
The field trial of embodiment 2:30% zineb Zhongshengmycin chitosan oligosaccharide wetting powder control citrus bacterial canker disease
Zineb 20%, Zhongshengmycin 4%, chitosan oligosaccharide 6%, alkyl benzene sulfonate calcium salt (dispersant) 7%, lauryl sodium sulfate (wetting agent) 4%, white carbon (filler) are added to 100%, mixture carries out drying, air-flow crushing, i.e. obtained 30% zineb Zhongshengmycin chitosan oligosaccharide wetting powder.
This formula is applied to control oranges and tangerines (fruit) canker.30% zineb Zhongshengmycin chitosan oligosaccharide wetting powder is diluted 2000 times by effective ingredient consumption, at citrus bacterial canker disease their early stage, even spraying is carried out to oranges and tangerines complete stool, do not drip for degree to squirt blade, sprayed once every 7-10 days, spray 3 times altogether, after last medicine, the control fruit of 15 days is 89.65%.Contrast medicament 80% Zineb wettable powder, 5% Zhongshengmycin wetting powder and 5% chitosan oligosaccharide wetting powder dilute 800 times, 300 times and 250 times by effective ingredient consumption respectively, adopt identical application method, after last medicine, the preventive effect of 15 days is respectively 70.46%, 72.35% and 63.52%.Zineb, Zhongshengmycin and the composite rear preventive effect to citrus bacterial canker disease of chitosan oligosaccharide are significantly better than contrasting single dose.
The field trial of embodiment 3:50% zineb Zhongshengmycin chitosan oligosaccharide wetting powder control citrus bacterial canker disease
Zineb 40%, Zhongshengmycin 4%, chitosan oligosaccharide 6%, alkylnaphthalene sulfonate (dispersant) 8%, neopelex (wetting agent) 5%, kaolin (filler) are added to 100%, mixture carries out drying, air-flow crushing, i.e. obtained 50% zineb Zhongshengmycin chitosan oligosaccharide wetting powder.
This formula is applied to control oranges and tangerines (fruit) canker.50% zineb Zhongshengmycin chitosan oligosaccharide wetting powder is diluted 3000 times by effective ingredient consumption, at citrus bacterial canker disease their early stage, even spraying is carried out to oranges and tangerines complete stool, do not drip for degree to squirt blade, sprayed once every 7-10 days, spray 3 times altogether, after last medicine, the control fruit of 15 days is 87.54%.Contrast medicament 80% Zineb wettable powder, 5% Zhongshengmycin wetting powder and 5% chitosan oligosaccharide wetting powder dilute 1000 times, 300 times and 250 times by effective ingredient consumption respectively, adopt identical application method, after last medicine, the preventive effect of 15 days is respectively 73.52%, 75.46% and 62.82%.Zineb, Zhongshengmycin and the composite rear preventive effect to citrus bacterial canker disease of chitosan oligosaccharide, significantly better than the preventive effect contrasting single dose, have good synergistic effect, have no adverse effects in test scope of medication to target crop.
Claims (3)
1. contain a bactericidal composition for Zhongshengmycin and chitosan oligosaccharide, it is characterized in that: active ingredient is made up of zineb, Zhongshengmycin and chitosan oligosaccharide, the ratio of quality and the number of copies of zineb, Zhongshengmycin and chitosan oligosaccharide is 10-20:2-3:1-3.
2. the bactericidal composition containing Zhongshengmycin and chitosan oligosaccharide according to claim 1, is characterized in that: active ingredient accounts for 30% ~ 60% of bactericidal composition gross weight.
3. the bactericidal composition containing Zhongshengmycin and chitosan oligosaccharide according to claim 1 and 2, is characterized in that: described bactericidal composition is prepared into wetting powder.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105104411A (en) * | 2015-07-28 | 2015-12-02 | 贵州大学 | Compound bactericide for preventing and treating tomato bacterial leaf spot |
| CN107258804A (en) * | 2017-07-24 | 2017-10-20 | 江苏明德立达作物科技有限公司 | A kind of microbicide compositions and its application |
| CN112493395A (en) * | 2020-11-27 | 2021-03-16 | 湖南湘华华大生物科技有限公司 | Irradiation sterilization method for tea |
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| CN103947661A (en) * | 2014-04-18 | 2014-07-30 | 北京明德立达农业科技有限公司 | Disease-resistant composition and application thereof |
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2015
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JPS63216804A (en) * | 1987-03-05 | 1988-09-09 | Meiji Seika Kaisha Ltd | Growth control of plant |
| CN101180962A (en) * | 2007-12-17 | 2008-05-21 | 东莞市瑞德丰生物科技有限公司 | Composition containing Zhongshengmycin and zineb with synergism |
| CN101816305A (en) * | 2010-05-09 | 2010-09-01 | 海南正业中农高科股份有限公司 | Bactericide pesticide composite containing oligochitosan |
| CN103947661A (en) * | 2014-04-18 | 2014-07-30 | 北京明德立达农业科技有限公司 | Disease-resistant composition and application thereof |
| CN104161041A (en) * | 2014-08-08 | 2014-11-26 | 黄业新 | Bactericidal composition containing amino-oligosaccharin and hexaconazole |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105104411A (en) * | 2015-07-28 | 2015-12-02 | 贵州大学 | Compound bactericide for preventing and treating tomato bacterial leaf spot |
| CN107258804A (en) * | 2017-07-24 | 2017-10-20 | 江苏明德立达作物科技有限公司 | A kind of microbicide compositions and its application |
| CN112493395A (en) * | 2020-11-27 | 2021-03-16 | 湖南湘华华大生物科技有限公司 | Irradiation sterilization method for tea |
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Effective date of registration: 20170309 Address after: 276100 Tancheng Middle Road, Shandong, No. 66 Applicant after: Zhu Dekun Address before: Binyang County of Nanning City, 530423 the Guangxi Zhuang Autonomous Region Wangling old Meng Yang Cun No. 13 Applicant before: Huang Yexin Applicant before: Yang Xinlan |
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