Embodiment
Below the specific embodiment of the present invention is elaborated.Should be understood that, embodiment described herein only, for description and interpretation the present invention, is not limited to the present invention.
In the present invention, the content sum of the each component in disease resistance composition is 100 % by weight.
The invention provides a kind of disease resistance composition; This disease resistance composition contains Zhongshengmycin and amino-oligosaccharide; With respect to the Zhongshengmycin of 1 weight portion, the content of amino-oligosaccharide is 0.1-80 weight portion.
Wherein, Zhongshengmycin is the trade name of the amino 1B-2 deoxidation gulose of compound 1-N glucoside base streptolidine base-2-amine, and English commodity are Zhongshengmycin, the antibiotic being produced by the mutation of lilac grey streptomycete Hainan, and molecular formula is C
19h
34o
8n
8, relative molecular weight is 502.0, structural formula is as the formula (1).
Wherein, amino-oligosaccharide, also claims agriculture special chitosan oligosaccharide, is the oligosaccharide that D-Glucosamine connects with β-Isosorbide-5-Nitrae glycosidic bond, and molecular weight can be 1000-5000, and preferred molecular weight is 2000-3000.
According to the present invention, the weight ratio of Zhongshengmycin and amino-oligosaccharide all has synergistic effect in wider scope, in order further to strengthen their synergistic effect, under preferable case, with respect to the Zhongshengmycin of 1 weight portion, the content of amino-oligosaccharide is 0.5-15 weight portion.
Wherein, in order further to strengthen their synergistic effect, under preferable case, with respect to the Zhongshengmycin of 1 weight portion, the content of amino-oligosaccharide is 1.5-5.5 weight portion.Under this preferable case, they the two there is outstanding synergistic effect, can obtain more excellent disease resistance effect with less disease resistance composition amount of application.
Wherein, disease resistance composition of the present invention can be made the formulations of pesticide of various routines, the multiple formulation of listing in GB/T19378-2003, for example wetting powder, water dispersible granules, suspending agent, oil-suspending agent, suspension emulsion, microcapsule suspending agent, missible oil, aqueous emulsion, microemulsion, granule, solvable pulvis or soluble granula.According to the difference of the formulations of pesticide, can use different auxiliary agents; Therefore,, as a kind of preferred embodiment of disease resistance composition of the present invention, disease resistance composition of the present invention also contains auxiliary agent.
For example, disease resistance composition of the present invention can be made water dispersible granules, rapidly disintegration be dispersed into the granular preparation of suspension after adding water; Wherein, the ratio of the gross weight of Zhongshengmycin and amino-oligosaccharide and the weight of auxiliary agent can be 1:(0.2-0.9).Described auxiliary agent can comprise at least one in dispersant, wetting agent, inert solid carrier and binding agent.Wherein, the weight ratio of the gross weight of Zhongshengmycin and amino-oligosaccharide and dispersant, wetting agent, inert solid carrier and binding agent can be 1:(0.06-0.22): (0.06-0.22): (0.18-0.65): (0.15-0.6).Wherein, described dispersant can comprise at least one in sodium methylene bis-naphthalene sulfonate, sodium lignin sulfonate, calcium lignosulfonate, polyoxyethylene ether phosphate, fatty alcohol-polyoxyethylene ether, anhydrous sorbitol APEO, dibenzyl phenol polyethenoxy ether, polyoxyethylene sorbitan ether monoleate, styryl phenol polyethenoxy ether and aliphatic amine polyoxyethylene ether; Described wetting agent can comprise at least one in lauryl sodium sulfate, calcium dodecyl benzene sulfonate, succinate sodium sulfonate, diisobutyl sodium naphthalene sulfonate (pulling open powder) and sldium lauryl sulfate.Wherein, described inert solid carrier can comprise at least one in clay, silica, diatomite, kaolin, attapulgite, imvite, bentonite, talcum powder, White Carbon black and calcium carbonate.Wherein, described binding agent can comprise at least one in sucrose, maltose, dextrin and starch.
Wherein, as the preferred a kind of embodiment of the present invention, disease resistance composition of the present invention is particularly suitable for preparing, storing and use with the formulation of wetting powder, can be scattered in the powderous preparations that forms stable suspension in water.
Wherein, be preferably in the embodiment of wetting powder in the formulation of disease resistance composition of the present invention, the ratio of the gross weight of Zhongshengmycin and amino-oligosaccharide and the weight of auxiliary agent can be 1:(0.2-10), more preferably 1:(0.4-20).
Wherein, be preferably in the embodiment of wetting powder in the formulation of disease resistance composition of the present invention, described auxiliary agent can comprise at least one in dispersant, wetting agent and inert solid carrier.Wherein, the weight ratio of the gross weight of Zhongshengmycin and amino-oligosaccharide and dispersant, wetting agent and inert solid carrier can be 1:(0.1-1): (0.1-1): (2-20).
Wherein, described dispersant can be the various interfacial agents with peptizaiton simultaneously in molecule with lipophile and two kinds of opposite nature of hydrophily; Be preferably in the embodiment of wetting powder in the formulation of disease resistance composition of the present invention, described dispersant can comprise at least one in sodium methylene bis-naphthalene sulfonate, sodium lignin sulfonate and calcium lignosulfonate.
Wherein, described wetting agent can be that various energy pass through to reduce its surface tension or interfacial tension, water can be deployed on solid material surface, or penetrate its surface, and material wetting solid material; Be preferably in the embodiment of wetting powder in the formulation of disease resistance composition of the present invention, described wetting agent can comprise at least one in lauryl sodium sulfate, calcium dodecyl benzene sulfonate, succinate sodium sulfonate, diisobutyl sodium naphthalene sulfonate (pulling open powder) and sldium lauryl sulfate.
Wherein, be preferably in the embodiment of wetting powder in the formulation of disease resistance composition of the present invention, described inert solid carrier can comprise at least one in clay, silica, diatomite, kaolin, attapulgite, imvite, bentonite, talcum powder, White Carbon black and calcium carbonate.
As the particularly preferred a kind of embodiment of the present invention, described disease resistance composition contains Zhongshengmycin and amino-oligosaccharide, and with respect to the Zhongshengmycin of 1 weight portion, the content of amino-oligosaccharide is 1.5-5.5 weight portion; Described disease resistance composition also contains sodium lignin sulfonate, lauryl sodium sulfate and diatomite; The ratio of the gross weight of Zhongshengmycin and amino-oligosaccharide and sodium lignin sulfonate, lauryl sodium sulfate and diatomaceous weight is 1:(0.2-0.8): (0.2-0.8): (5-11).The in the situation that of this preferred embodiment, described disease resistance composition can have wider disease resistance spectrum and higher preventive effect.
In the present invention, fatty alcohol-polyoxyethylene ether is as R-O-(CH
2cH
2o)
nshown in-H, the alkyl that R is saturated or unsaturated C12-C18, the integer that n is 10-20; Preferably, the alkyl that R is saturated or unsaturated C12-C15, the integer that n is 15-20.
In the present invention, in the structure of anhydrous sorbitol APEO, the number of oxyethylene group can be 20-80, is preferably 40-60.
The present invention also provides the purposes of disease resistance composition as above in the disease of control crops.
Wherein, described crops include but not limited at least one in tomato, eggplant, capsicum, potato and ginger; Described disease includes but not limited at least one in bacterial wilt and/or fusarium wilt.The disease of disease resistance composition particularly suitable of the present invention for preventing and treating the crops that comprise bacterial wilt of tomato and/or tomato wilt.
Wherein, above-mentioned disease resistance composition does not have special requirement at the consumption of every mu of ground, and for example can make the Zhongshengmycin of every mu of ground and total amount of application of amino-oligosaccharide is 4-6g.Above-mentioned disease resistance composition can dilute before use and use in the mode of spraying.It is 15-60L that the ratio of dilution can make the amount of spraying on every mu of ground.
Each component in above-mentioned disease resistance composition can be separated and independently deposited, and is mixing before use.In another embodiment, the two or more components in above-mentioned composition pesticide can mix as mixture, provide in the mode of mixture.
Further describe by the following examples the present invention.
In following examples, Zhongshengmycin is purchased from Xiamen Kai Li biological products Co., Ltd; Amino-oligosaccharide is purchased from Hua Nuo bio tech ltd, Weifang, and molecular weight is 2000-3000; Fatty alcohol-polyoxyethylene ether is purchased from blue star auxiliary reagent factory, Xingtai, and alkyl carbon number is C12-C15, and oxyethylene group number is 12-50; Sodium lignin sulfonate is purchased from Tianjin Heowns Biochemical Technology Co., Ltd.; Calcium lignosulfonate is purchased from Tianjin Heowns Biochemical Technology Co., Ltd.; Calcium dodecyl benzene sulfonate is purchased from Jinan Hao Hua limited industrial company; Lauryl sodium sulfate is purchased from novel river, Hubei Chemical Co., Ltd..
Embodiment 1
The present embodiment is prepared the disease resistance composition that formulation is wetting powder.
Get the Zhongshengmycin of 2.5 weight portions, the amino-oligosaccharide of 7.5 weight portions, sodium lignin sulfonate, the lauryl sodium sulfate of 5 weight portions and the diatomite of 80 weight portions of 5 weight portions and mix, obtain the disease resistance composition of the present embodiment.
Embodiment 2
The present embodiment is prepared the disease resistance composition that formulation is wetting powder.
Get the Zhongshengmycin of 4 weight portions, the amino-oligosaccharide of 6 weight portions, sodium lignin sulfonate, the lauryl sodium sulfate of 5 weight portions and the diatomite of 80 weight portions of 5 weight portions and mix, obtain the disease resistance composition of the present embodiment.
Embodiment 3
The present embodiment is prepared the disease resistance composition that formulation is wetting powder.
Get the Zhongshengmycin of 1.6 weight portions, the amino-oligosaccharide of 8.4 weight portions, sodium lignin sulfonate, the lauryl sodium sulfate of 5 weight portions and the diatomite of 80 weight portions of 5 weight portions and mix, obtain the disease resistance composition of the present embodiment.
Embodiment 4
The present embodiment is prepared the disease resistance composition that formulation is wetting powder.
Get the Zhongshengmycin of 6.7 weight portions, the amino-oligosaccharide of 3.3 weight portions, calcium lignosulfonate, the lauryl sodium sulfate of 5 weight portions and the diatomite of 80 weight portions of 5 weight portions and mix, obtain the disease resistance composition of the present embodiment.
Embodiment 5
The present embodiment is prepared the disease resistance composition that formulation is wetting powder.
Get the Zhongshengmycin of 0.625 weight portion, the amino-oligosaccharide of 9.375 weight portions, sodium lignin sulfonate, the calcium dodecyl benzene sulfonate of 5 weight portions and the diatomite of 80 weight portions of 5 weight portions and mix, obtain the disease resistance composition of the present embodiment.
Embodiment 6
The present embodiment is prepared the disease resistance composition that formulation is wetting powder.
Get the Zhongshengmycin of 9 weight portions, the amino-oligosaccharide of 1 weight portion, sodium lignin sulfonate, the lauryl sodium sulfate of 5 weight portions and the diatomite of 80 weight portions of 5 weight portions and mix, obtain the disease resistance composition of the present embodiment.
Embodiment 7
The present embodiment is prepared the disease resistance composition that formulation is wetting powder.
Get the Zhongshengmycin of 0.15 weight portion, the amino-oligosaccharide of 9.85 weight portions, sodium lignin sulfonate, the lauryl sodium sulfate of 5 weight portions and the diatomite of 80 weight portions of 5 weight portions and mix, obtain the disease resistance composition of the present embodiment.
Embodiment 8
The present embodiment is prepared the disease resistance composition that formulation is water dispersible granules.
Get the Zhongshengmycin of 15 weight portions, the amino-oligosaccharide of 45 weight portions, the fatty alcohol-polyoxyethylene ether of 3 weight portions, the sodium lignin sulfonate of 5 weight portions, lauryl sodium sulfate, the starch of 5 weight portions and the diatomite of 22 weight portions of 5 weight portions and mix, obtain the disease resistance composition of the present embodiment.
Comparative example 1
Zhongshengmycin, the sodium lignin sulfonate of 5 weight portions and the diatomite of 85 weight portions of getting 10 weight portions mix, and obtain the disease resistance composition of the wetting powder of this comparative example.
Comparative example 2
Get the amino-oligosaccharide of 10 weight portions, sodium lignin sulfonate, the lauryl sodium sulfate of 5 weight portions and the diatomite of 80 weight portions of 5 weight portions mixes, and obtains the disease resistance composition of the wetting powder of this comparative example.
Comparative example 3
Get the Zhongshengmycin of 2.5 weight portions, the chitin of 7.5 weight portions (purchased from Henan Xing Yuan chemical products Co., Ltd, molecular weight is about 8000), sodium lignin sulfonate, the lauryl sodium sulfate of 5 weight portions and the diatomite of 80 weight portions of 5 weight portions mixes, and obtains the disease resistance composition of the wetting powder of this comparative example.
Comparative example 4
Get the antibiotic in pyrimidine nucleoside class of 2.5 weight portions (purchased from Zhejiang Sheng Longnonghua Science and Technology Ltd., article number is pesticide corrosion 120), the amino-oligosaccharide of 7.5 weight portions, sodium lignin sulfonate, the lauryl sodium sulfate of 5 weight portions and the diatomite of 80 weight portions of 5 weight portions mix, and obtains the disease resistance composition of the wetting powder of this comparative example.
Test implementation example 1
This test implementation example is tested the control efficiency of bacterial wilt of tomato.
Infecting the tomato planting district build-in test embodiment 1-8 of bacterial wilt of tomato and the control efficiency of the disease resistance composition of comparative example 1-4 to bacterial wilt of tomato, concrete steps comprise: the disease resistance composition of blade face spray application embodiment 1-8 and comparative example 1-4 in different growing areas.Wherein, the amount of application of the disease resistance composition of embodiment 1-7 and comparative example 1-4 is 50g/ mu, and the amount of application of the disease resistance composition of embodiment 8 is 8.3g/ mu.The area of testing the growing area of every kind of disease resistance composition is 0.2 mu, while using, being diluted with water to every mu of spray amount is 30L, and substitute disease resistance composition as space management using water, except using by such scheme disease resistance composition, other planting conditions of each growing area is identical.Insecticide spraying time is their early stage, and be 7 days interval, continuous spraying medicine 3 times, and last 7 days " Invest, Then Investigate "s of dispenser, carry out classification record taking strain as unit, calculate disease index, control efficiency, the results are shown in Table 1.Severity Scaling standard comprises: 0 grade: complete stool is anosis.1 grade: leaf color shoals, or top 1/3 blade is wilting shape.2 grades: 1/3-1/2 blade is wilting shape, diseased plant is downgraded as the more than 2/3 of normal plant height.3 grades: 1/2-2/3 blade is wilting shape, diseased plant is downgraded the 2/3-1/2 left and right for normal plant height.4 grades: complete stool blade is wilting shape, or withered, diseased plant downgrade for normal plant height more than 1/2 to 1/3.Calculate disease index and preventive effect according to formula (A) and formula (B).
Table 1
Disease resistance composition |
Bacterial wilt of tomato control efficiency (%) |
Embodiment 1 |
90.39 |
Embodiment 2 |
89.83 |
Embodiment 3 |
89.26 |
Embodiment 4 |
75.86 |
Embodiment 5 |
72.37 |
Embodiment 6 |
47.12 |
Embodiment 7 |
45.95 |
Embodiment 8 |
80.79 |
Comparative example 1 |
26.85 |
Comparative example 2 |
24.37 |
Comparative example 3 |
21.77 |
Comparative example 4 |
13.54 |
Test implementation example 2
This test implementation example is tested the control efficiency of tomato wilt.
Infecting the tomato planting district build-in test embodiment 1-8 of tomato wilt and the control efficiency of the disease resistance composition of comparative example 1-4 to tomato wilt, concrete steps comprise: the disease resistance composition of blade face spray application embodiment 1-8 and comparative example 1-4 in different growing areas.Wherein, the amount of application of the disease resistance composition of embodiment 1-7 and comparative example 1-4 is 50g/ mu, and the amount of application of the disease resistance composition of embodiment 8 is 8.3g/ mu.The area of testing the growing area of every kind of disease resistance composition is 0.2 mu, while using, being diluted with water to every mu of spray amount is 30L, and substitute disease resistance composition as space management using water, except using by such scheme disease resistance composition, other planting conditions of each growing area is identical.Insecticide spraying time is their early stage, and be 7 days interval, continuous spraying medicine 3 times, and last 7 days " Invest, Then Investigate "s of dispenser, carry out classification record taking strain as unit, calculate disease index, control efficiency, the results are shown in Table 2.Severity Scaling standard comprises: 0 grade: complete stool is anosis; 1 grade: the flavescence of 1-2 sheet leaf, come off; 2 grades: 1-2 sheet leaf yellowing or complete stool blade become yellow green, blade is sagging is slight wilting; 3 grades: complete stool is obviously wilted or the serious flavescence of blade, plant strain growth is obstructed, slightly downgrades; 4 grades: complete stool blade is seriously withered even withered.
Table 2
Disease resistance composition |
Tomato wilt control efficiency (%) |
Embodiment 1 |
90.75 |
Embodiment 2 |
90.19 |
Embodiment 3 |
89.62 |
Embodiment 4 |
76.16 |
Embodiment 5 |
72.66 |
Embodiment 6 |
47.31 |
Embodiment 7 |
46.13 |
Embodiment 8 |
81.11 |
Comparative example 1 |
26.96 |
Comparative example 2 |
24.47 |
Comparative example 3 |
21.86 |
Comparative example 4 |
13.59 |
As can be seen from Table 1 and Table 2, disease resistance composition of the present invention is due to its distinctive composition and combination, there is very notable synergistic effect, can obtain the effect of preventing and treating efficiently disease with respect to the combination of other composition and consumption, thereby improve the output of crops.
In addition, as can also be seen from Table 1, disease resistance composition of the present invention is at the preferred Zhongshengmycin with respect to 1 weight portion, the content of amino-oligosaccharide is in the situation of 0.5-15 weight portion, and particularly, at the preferred Zhongshengmycin with respect to 1 weight portion, the content of amino-oligosaccharide is in the situation of 1.5-5.5 weight portion, can obtain more excellent control efficiency, and can reduce dosage, cost-saving, alleviate the pollution to environment and improve crop yield.
More than describe the preferred embodiment of the present invention in detail; but the present invention is not limited to the detail in above-mentioned embodiment, within the scope of technical conceive of the present invention; can carry out multiple simple variant to technical scheme of the present invention, these simple variant all belong to protection scope of the present invention.
It should be noted that in addition, each concrete technical characterictic described in above-mentioned embodiment, in reconcilable situation, can combine by any suitable mode, for fear of unnecessary repetition, the present invention is to the explanation no longer separately of various possible combinations.
In addition, between various embodiment of the present invention, also can be combined, as long as it is without prejudice to thought of the present invention, it should be considered as content disclosed in this invention equally.