CN101816305A - Bactericide pesticide composite containing oligochitosan - Google Patents
Bactericide pesticide composite containing oligochitosan Download PDFInfo
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Abstract
The invention discloses a bactericide pesticide composite containing oligochitosan, which comprises a component A and a component B, wherein the component A is oligochitosan or a derivative of the oligochitosan; and the component B is one or several of active compounds. The pesticide composition can produce a synergistic action, reduce the dosage of a chemically synthetic bactericide, prevent or delay the medicament resistance of the bactericide, improve crop stress resistance and yield, regulate metabolic balance in plant bodies and improve quality of agricultural products, improve the application safety of the bactericide and is more safe to ecological environmental and human bodies.
Description
Technical field
The present invention relates to the agricultural chemicals applied technical field, relate in particular to a kind of composition pesticide that improves vegetation growth state.
Background technology
Chemical pesticide is protected in the receipts at agricultural produce with quick effective and cheap characteristics and has been brought into play huge effect, but scientifically arbitrarily do not use, make the continuous increase of drug-fast continuous enhancing of pathogen and pathogenic mutation, cause that agricultural chemicals pesticide resistance, secondary insect break out greatly, residue of pesticide, environmental pollution and a series of serious ecological problems of agricultural product security and social concern.And, the quality safety of agricultural product and the quality of agricultural product are had higher requirement along with the improving constantly of people's living standard.Therefore, how to carry out the science medication, reduce the usage amount of chemical pesticide; improve the service efficiency of agricultural chemicals, when reducing plant pest harm, strengthen self resistance of crop; when improving crop yield, improve quality of agricultural product, become the problem that solution is badly in need of in the plant protection field.
The eighties in 20th century, it is found that the oligosaccharides material that derives from fungi and plant cell wall can open multiple signal transduction path in the plant, as research and development, the production of the brand-new biological exciton of a class (containing oligosaccharides, glycoprotein, polypeptide and fatty acid material) biochemistry agricultural chemicals and use and caused showing great attention to of external scientific and technological circle and international transnational major company.Oligosaccharides is meant the compound that is formed by connecting by glycosidic bond by 2~30 monose molecules, in life entity, mainly being that form with glycoprotein, glycolipid and glycopeptide glycoconjugate participates in many vital movements, often is exactly those oligosaccharides residues and these glycoconjugates play a decisive role in the process of performance biological function.Oligosaccharides also is present in simultaneously in the cell membrane of zooblast (the whole cell membrane surface all is coated with oligosaccharides); these oligosaccharides all play an important role in the normal or abnormal identifying of cell, thereby many microorganisms also are to infect beginning by combining with the host cell surface sugar chain.
Chitosan oligosaccharide also is Chitosan poly oligosaccharide, also claims chitin oligo saccharide, formal name used at school β-1, and 4-oligosaccharides-gucosamine is the bioactivator with specific aggregation degree that shitosan is obtained through special biological enzyme technology processing.Its molecular weight is lower, and is water-soluble better, easily absorbed by organism, has special biologically active.Chitosan oligosaccharide is a newfound in the world class signaling molecule, has the function of diseases prevention, disease-resistant, regulating plant growth, growth, proliferation, utilizes the chitosan oligosaccharide of separate sources to develop serial oligosaccharides biopesticide at all kinds of diseases.
Summary of the invention
The objective of the invention is to: a kind of bactericide pesticide composite that contains chitosan oligosaccharide that improves stress resistance of plant, raising plant inducer adpedance characteristic of disease, improves plant product quality and promotion plant growing is provided.
To achieve these goals, technical scheme of the present invention is: a kind of bactericide pesticide composite that contains chitosan oligosaccharide is provided, wherein, comprises component A and B component, described component A is chitosan oligosaccharide or chitosan oligosaccharide derivative, B component be selected from the following reactive compound wherein one or more:
(1) heterocyclic nitrogen-group fungicide: nitrile bacterium azoles, alkene azoles alcohol, Triadimenol, Bitertanol, own azoles alcohol, Difenoconazole, Flusilazole, propiconazole, Tebuconazole, alkene azoles alcohol, triazolone, flumorph, dimethomorph, tridemorph, Difenoconazole, carbendazim, iprodione, procymidone, pefurazoate, Prochloraz, prochloraz nantokite or prochloraz-manganese chloride complex, the mould spirit of evil, mepanipyrim, captan, flumorph, azolactone bacterium, different thiophene bacterium ketone, Fenamidone;
(2) organic phosphorus type disinfectant: tolelofos-methyl, iprobenfos, kitazine, three second aluminium phosphates;
(3) substituted beneze-group fungicides: metalaxyl, tpn, Experimental chemotherapeutant 1182, thiophanate methyl, fenaminosulf;
(4) organic sulfur series bactericidal agent: carbendazim, methyl Tobe rule, S-Ethyl ethylthio sulfonate, tmtd, ziram, mancozeb, zineb;
(5) methoxy acrylate series bactericidal agent: kresoxim-methyl, Fluoxastrobin, Enestroburin, pyraclostrobin, ZEN 90160;
(6) antibiotics bactericide: kasugarnycin, Polyoxin, Ningnanmycin, jinggangmycin, streptomycin;
(7) organochlorine bactericide: tpn, pcnb, Pencycuron;
(8) other series bactericidal agent: Propamocarb, the mould prestige of second, white urea cyanogen, bromine bacterium are clear.
Preferably, the percentage that this component A accounts for gross weight is 0.1%-40%, and the percentage that B component accounts for gross weight is 0.1%-99%.
Preferably, this component A is that the degree of polymerization is chitosan oligosaccharide or the chitosan oligosaccharide derivative of 2-30.
Preferably, this composition pesticide can be made into the following formulations of pesticide: wherein a kind of of aqua, soluble liquid, missible oil, microemulsion, aqueous emulsion, wetting powder, water dispersion granule, suspending agent, granule, paste, controlled release formulation of capsule, tablet.
The invention has the advantages that:
(1) produces synergistic function, reduce the consumption of chemosynthesis bactericide, overcome or delayed the bactericide pesticide resistance;
(2) improve the resistance (inducing anti-disease, cold-resistant, non-irrigated, waterlogging, salt) of crop self, the raising crop yield;
(3) regulate plant corpus inner metabolism balance, improve the quality (variation of secondary metabolites in the plant corpus, the change of vitamin, organic acid equal size) of agricultural product;
(4) strengthen bactericide and used safety, safer to the ecotope and the mankind.
Among the present invention effective degree of polymerization of chitosan oligosaccharide be 2-30 (for example chitobiose, chitotriose, shell tetrose ..., shell 29 oligosaccharides, shell 30 oligosaccharides), preferred version of the present invention is the chitosan oligosaccharide (be chitobiose, chitotriose, shell tetrose, shell pentasaccharides, shell six sugar, shell seven sugar, shell eight sugar, shell nine sugar, shell ten sugar) of the degree of polymerization at 2-10; Among the present invention chitosan oligosaccharide and the composition of bactericide be single chitosan oligosaccharide or multiple chitosan oligosaccharide simultaneously with the present invention in relevant bactericide use.
Mesochite oligosaccharide derivative of the present invention is meant chitosan oligosaccharide ester derivative, the rare earth derivative that forms by esterification, complexation reaction.
The composition that comprises component A and B component of the present invention, can mix and use or separate administration, the composition application process for the spraying that is used for plant, plant shoot branch (flower, leaf, stem, really, handle), smear, sprinkle handles, the seed soaking of seed, dressing, seed dressing are handled, and the spray in plant root or plantation place, irrigate, disinfect.These processing mainly are to improve vegetation growth state, and application process comprises to be used or separate administration simultaneously.The vegetation growth state improvement is mainly reflected in following aspect among the present invention: it is (for example cold-resistant to improve stress resistance of plant, freeze proof, drought resisting, anti-salt, waterlogging-resistant, hot-dry wind tolerance), improve plant inducer adpedance characteristic of disease (flavones in the plant corpus for example, the content of terpene secondary metabolism product increases) and improve the plant product quality and (for example change crop vitamin, protein, organic acid, pigment content), promote plant growing (seed germination speed for example, plant height changes, root system changes, tiller and increase and the leaf growth variation), the flowers and fruits growth alteration (is for example bloomed early, fruit maturation is early), output improves.For example, we find, when protecting the ground capsicum with the compositions-treated that comprises component A and B component, can obviously reduce the incidence of disease of capsicum epidemic disease, anthracnose, and single cropping reduces bactericide with 2-3 time, reduces the bactericide consumption more than 40%; Simultaneously, obviously strengthen the cold tolerance of capsicum, outstanding behaviours increases capsicum setting quantity exactly, and the capsicum volume increase of using the compositions-treated that comprises component A and B component is more than 30%; Simultaneously, the pepper fruit skin hardness obviously strengthens, and is fit to long-distance transportation more.
When the composition that comprises component A and B component among the present invention uses as a kind of preparation, can be processed into all kinds of formulations of pesticide, wherein a kind of of the preferred microemulsion of the present invention, aqueous emulsion, wetting powder, water dispersion granule, suspending agent, granule, paste, controlled release formulation of capsule, tablet.
Embodiment
The invention will be further described below in conjunction with preferred embodiment, but the present invention never is limited to following embodiment.
The preparation of embodiment 1:1% chitosan oligosaccharide+10% myclobutanil micro-emulsion pharmaceutical formulation, each set of dispense ratio is:
Chitosan oligosaccharide 1%
Nitrile bacterium azoles 10%
Surfactant: alkylphenol polyoxyethylene 20%
Cosolvent: methyl alcohol, cyclohexanone 10%
Stabilizing agent: ethylene glycol 5%
Solvent: running water surplus
With each component weighing total amount in proportion is 100%, fully stirs, and mixes well and promptly gets product
The configuration of embodiment 2:2% chitosan oligosaccharide+60% thiram wettable powder preparation, the proportioning of each component is:
Chitosan oligosaccharide 2%
Tmtd 60%
Wetting agent: neopelex 10%
Dispersant: NNO 5%
Filler: white carbon surplus
With individual component drying, mixing, carry out comminution by gas stream, the levigate product that promptly gets.
The preparation of embodiment 3:2% chitosan oligosaccharide+10% iprodione water dispersible granular agent formulation, the proportioning of each component is:
Chitosan oligosaccharide 2%
Iprodione 10%
Wetting agent: neopelex 10%
Dispersant: calcium lignosulfonate 10%
Binding agent: gum Arabic 1%
Disintegrant: ammonium sulfate 1%
Carrier: kaolin surplus
The mixture of preparation is in proportion carried out ultra-fine grinding, granulation, dry, screening promptly gets product.
The preparation of embodiment 4:2% chitosan oligosaccharide+25% Fluoxastrobin suspending agent preparation, the proportioning of each component is:
Chitosan oligosaccharide 2%
Fluoxastrobin 25%
Wetting agent: alkylaryl sulfonates 10%
Dispersant: draw back powder 7%
Tackifier: CMC 1%
Stabilizing agent: Magnesiumaluminumsilicate 0.5%
Defoamer: silicone defoaming agent 0.2%
Antifreezing agent: ethylene glycol 5%
Solvent: running water surplus
Each component is mixed well, carry out high speed shear, grind, reach certain fineness, promptly get product with sand mill.
The preparation of embodiment 5:0.5% chitosan oligosaccharide+15% carbendazim granular preparation, the proportioning of each component is:
Chitosan oligosaccharide 0.5%
Carbendazim 15%
Wetting agent: neopelex 15%
Dispersant: sodium lignin sulfonate 10%
Binding agent: cyclodextrin 5%
Disintegrant: starch 2%
Carrier: dolomite surplus
The mixture of preparation is in proportion carried out granulation after the ultra-fine grinding, and dry, screening promptly gets product.
The preparation of embodiment 6:1% chitosan oligosaccharide+10% triazolone paste preparation, the proportioning of each component is:
Chitosan oligosaccharide 1%
Triazolone 10%
Surfactant: phenethyl phenol APEO 20%
Cosolvent: dimethylbenzene 10%
Antifreezing agent thickener: sodium alginate 6%
Solvent: running water surplus
Each component is mixed well and fully stirred, promptly get product.
The composition pesticide application process and the test effect that comprise component A and B component are as follows:
Embodiment 1: the composition AB-1 that comprises component A and B component prevents and treats sigatoka
Handle medicament: comprise the composition AB-1 of component A and B component, component A (shell pentasaccharides) 1% among the AB-1 wherein, B component (propiconazole) 15% is mixed with microemulsion;
Contrast medicament: 25% propiconazole microemulsion, 20% Difenoconazole aqueous emulsion
Trial crops: banana (becoming the strain phase).
Controlling object: sigatoka
Processing method: the banana ground of selection sigatoka initial stage of origination, establish 2 concentration respectively to handling medicament and contrasting medicament, 3 repeat the spraying processing, and the field efficacy statistics is carried out in dispenser after 72 hours.
Control efficiency sees Table 1.
Table 1AB-1 prevents and treats the sigatoka effect
As can be seen from Table 1, the composition AB-1 that comprises component A and B component is when preventing and treating sigatoka, and component A and B component have obvious synergistic function.
Embodiment 2: the composition AB-2 control cucumber downy mildew that comprises component A and B component
Handle medicament: comprise the composition AB-2 of component A and B component, component A (chitotriose) 2% among the AB-1 wherein, B component (Fluoxastrobin) 15% is mixed with agricultural chemicals suspension agent;
Contrast medicament: 25% Fluoxastrobin suspending agent, 40% tpn suspending agent
Trial crops: cucumber (becoming the strain phase).
Controlling object: cucumber downy mildew
Processing method: the plot of selection cucumber downy mildew initial stage of origination, establish 2 concentration respectively to handling medicament and contrasting medicament, 3 repeat the spraying processing, and the field efficacy statistics is carried out in dispenser after 48 hours.
Control efficiency sees Table 2.
Table 2AB-2 control cucumber downy mildew effect
As can be seen from Table 2, the composition AB-1 that comprises component A and B component is when the control cucumber downy mildew, and component A and B component have obvious synergistic function.
Embodiment 3: with component A and B component associated treatment navel orange
Handling medicament: component A is chitosan oligosaccharide, and B component is (80% carbendazol wettable powder, 80% mancozeb wettable powder, 10% difenoconazole water dispersible granule, 25% a Prochloraz missible oil);
Control treatment: B component
Trial crops: navel orange
Handle object: navel orange season of growth disease.
Processing method: in the season of growth of navel orange, a situation arises according to navel orange field disease, and since March, a situation arises according to the navel orange disease at control group, respectively with the processing of spraying of different B component; In processed group, the B component of selecting the concurrent control group to use, and add the component A processing of spraying simultaneously; When disease-free generation of processed group or generation are light, just stop to use B component.When navel orange becomes private school the fruit inclusion is measured.
Result statistics: from field trial, growth of control group navel orange is wanted medication season 4 times, and processed group medication 3 times, and can control all kinds of diseases generations of navel orange well.Navel orange inclusion after gathering is analyzed, and the navel orange organic acid content of component A and B component associated treatment obviously reduces, and Vc content obviously improves (seeing Table 3), and promptly the inclusion of navel orange and contrast obviously change, and the navel orange quality is significantly improved.
Table 3 oligosaccharides is to the influence of navel orange inclusion
Edible rate | Titratable acid | The solubility total reducing sugar | Vc content (mg/100mL) | Soluble solid | Gu acid ratio | |
Contrast | ??78.46% | ??1.13% | ??5.26% | ??39.16 | ??13.80% | ??11.94 |
Oligosaccharides is handled | ??73.56% | ??0.88% | ??5.93% | ??46.93 | ??14.66% | ??18.14 |
State's scale value | ??≥70%?? | ??≥0.9% | ??≥10% | ??≥9.0 | ||
Rate of change | ??- | ??↓21.43% | ??↑12.74% | ??↑19.49% | ??↑6.25% | ??↑51.93% |
Annotate: Jiangxi Province, (1) this experiment place.
(2) state's scale value derives from GBT 20355-2006 geographical sign product Jiangxi south navel orange and south, DB 36T389-2003 pollution-free food Jiangxi navel orange.
Claims (4)
1. bactericide pesticide composite that contains chitosan oligosaccharide, it is characterized in that: comprise component A and B component, described component A is chitosan oligosaccharide or chitosan oligosaccharide derivative, B component be selected from the following reactive compound wherein one or more:
(1) heterocyclic nitrogen-group fungicide: nitrile bacterium azoles, alkene azoles alcohol, Triadimenol, Bitertanol, own azoles alcohol, Difenoconazole, Flusilazole, propiconazole, Tebuconazole, alkene azoles alcohol, triazolone, flumorph, dimethomorph, tridemorph, Difenoconazole, carbendazim, iprodione, procymidone, pefurazoate, Prochloraz, prochloraz nantokite or prochloraz-manganese chloride complex, the mould spirit of evil, mepanipyrim, captan, flumorph, azolactone bacterium, different thiophene bacterium ketone, Fenamidone;
(2) organic phosphorus type disinfectant: tolelofos-methyl, iprobenfos, kitazine, three second aluminium phosphates;
(3) substituted beneze-group fungicides: metalaxyl, tpn, Experimental chemotherapeutant 1182, thiophanate methyl, fenaminosulf;
(4) organic sulfur series bactericidal agent: carbendazim, methyl Tobe rule, S-Ethyl ethylthio sulfonate, tmtd, ziram, mancozeb, zineb;
(5) methoxy acrylate series bactericidal agent: kresoxim-methyl, Fluoxastrobin, Enestroburin, pyraclostrobin, ZEN 90160;
(6) antibiotics bactericide: kasugarnycin, Polyoxin, Ningnanmycin, jinggangmycin, streptomycin;
(7) organochlorine bactericide: tpn, pcnb, Pencycuron;
(8) other series bactericidal agent: Propamocarb, the mould prestige of second, white urea cyanogen, bromine bacterium are clear.
2. the bactericide pesticide composite that contains chitosan oligosaccharide as claimed in claim 1 is characterized in that: the percentage that this component A accounts for gross mass is 0.1%-40%, and the percentage that B component accounts for gross mass is 0.1%-99%.
3. the bactericide pesticide composite that contains chitosan oligosaccharide as claimed in claim 1 is characterized in that: this component A is that the degree of polymerization is chitosan oligosaccharide or the chitosan oligosaccharide derivative of 2-30.
4. the bactericide pesticide composite that contains chitosan oligosaccharide as claimed in claim 1 is characterized in that: this composition pesticide can be made into the following formulations of pesticide: wherein a kind of of aqua, soluble liquid, missible oil, microemulsion, aqueous emulsion, wetting powder, water dispersion granule, suspending agent, granule, paste, controlled release formulation of capsule, tablet.
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