CN109964960A - Composition containing toVerticilliumdahliaActivitie Activitie S of Relative activator protein and allantoin - Google Patents
Composition containing toVerticilliumdahliaActivitie Activitie S of Relative activator protein and allantoin Download PDFInfo
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- CN109964960A CN109964960A CN201910252379.8A CN201910252379A CN109964960A CN 109964960 A CN109964960 A CN 109964960A CN 201910252379 A CN201910252379 A CN 201910252379A CN 109964960 A CN109964960 A CN 109964960A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/36—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< directly attached to at least one heterocyclic ring; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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- Microbiology (AREA)
- Pest Control & Pesticides (AREA)
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Abstract
The invention discloses a kind of composition pesticide with synergistic effect, effective component includes A and B, and wherein active component A is toVerticilliumdahliaActivitie Activitie S of Relative activator protein, and active component B is allantoin, and weight ratio is 1:1~300.The present invention also provides the Preparation method and uses of the composition.Test result show composition of the invention can good regulating crop growth, improve disease-resistant resistance, promote crop health benefit, improve crop yield.
Description
Technical field
The invention belongs to agriculture plant protection fields, more particularly to a kind of with the composition pesticide for improving performance, tool
Say to body it is to be related to a kind of composition comprising toVerticilliumdahliaActivitie Activitie S of Relative activator protein and allantoin.
Background technique
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein (plant activator protein) is to screen from Alternariaspp, separate, is pure
A kind of novel protein dissolved improves plant autoimmunity mainly by molecular immune system in activated plant body；By swashing
A series of intracorporal metabolic regulations of plant are sent out, plant roots, stem, leaf growth are promoted and improve chlorophyll content, improve crop yield,
There is higher preventive effect to plurality of plant diseases.
Allantoin (allantoin) is a kind of broad spectrum activity plant growth regulator.Allantoin can enhance the activity of invertase,
Improve sugarcane yield；Allantoin has activation to edaphon, to there is the effect for improving soil；Due to that can draw after application
The variation for playing plant nucleic acid in vivo plays the role of multiple kinds of crops to promote growth.To rice, corn, wheat, apple, grape,
The crops such as citrus, peach, Lee, lichee, capsicum, soybean, peanut, melon have significant effect of increasing production, and have solid fruit, precocious effect.
The plant growth regulator of commercial type is mainly based on single dose at present, and function and effect are single, common
Plant growth regulator compounding security risks it is very big, be easy to cause plant growth deformity, runt seedling, shedding, yield decline and
Quality reduces.
Summary of the invention
The object of the present invention is to provide a kind of with collaboration promotion plant growth, improves crop yield and has certain anti-
The composition of disease performance.Inventors have found that toVerticilliumdahliaActivitie Activitie S of Relative activator protein and allantoin are adjusting between the two or are promoting to plant
Object growth aspect has significant synergistic function.
The purpose of the present invention can be achieved by the following measures:
A kind of composition, the composition include two kinds of active components of A and B, and wherein active component A is toVerticilliumdahliaActivitie Activitie S of Relative activation
Albumen, active component B are allantoin, and the weight ratio of active component A and active component B are 1:1~300.In a kind of preferred embodiment
In, the weight ratio of active component A and active component B are 1:5~275.
The dosage form allowed on pesticide can be made by active constituent and insecticides adjuvant or auxiliary material in composition of the invention.Into one
Step, the composition forms agriculture by the active component of 1~85% parts by weight and the insecticides adjuvant of 99~15% parts by weight or auxiliary material
The dosage form allowed on medicine or fertilizer.
Inventor is found by experiment that composition of the invention can preferably adjust the growth of crop, promotes crop
Health benefit improves the yield of crop.
The present invention provides the compositions comprising component A (toVerticilliumdahliaActivitie Activitie S of Relative activator protein) and component B (allantoin) in agriculture
Industry field adjusts the purposes in crop growth, especially purposes in terms of regulating crop growth improves.
The dosage form that pesticide can be made in composition of the invention of effective component and insecticides adjuvant or fertilizer preparation allows.
To give full play to advantageous effect of the invention, composition of the invention can also (such as fungicide be killed with other pesticides
Worm agent, herbicide, plant growth regulator), fertilizer etc. be used in mixed way, these therewith it is mixed be disclosed in the prior art often
Pesticide or fertilizer.
It may include one or more kinds of microbial bacterias in composition of the invention to further increase using effect
Agent can usually be processed into certain particular dosage form, such as aqua or granule liquid or solid dosage form jointly with microbial bacterial agent,
After dosage form can also being made containing effective component of the invention, it is co-administered in application with microbial bacterial agent.It is suitable micro-
Bacteria agent is bacillus category microbial bacterial agent, further preferably include bacillus subtilis, bacillus licheniformis,
One of bacillus thuringiensis, waxy Bacillus, solution shallow lake bacillus, bacillus laterosporus etc. or more than one is mixed
Close microorganism fungus kind.The living bacteria count total amount of microbial bacterial agent be greater than 200,000,000/gram, preferably living bacteria count total amount be greater than 1,000,000,000/
Gram.Further, composition of the invention can also contain organic in the presence of common with microbial bacterial agent, in this combination
Matter.The content of organic matter is greater than 8%, preferably higher than 20%.
Composition of the invention, it is suitable to be processed into jointly with conventional a great number of elements, moderate-element, microelement etc.
Dosage form, after two effective components of the invention can also being processed into suitable dosage form, as needed in application with it is conventional big
Secondary element, moderate-element, microelement etc. are used in conjunction with.
When as Pesticide use, above-mentioned composition specifically may include insecticides adjuvant, such as carrier, solvent, dispersing agent, wetting
One or more of agent, adhesive, thickener, adhesive, surfactant etc. can mix common during application
Auxiliary agent.
Suitable auxiliary agent can be solid or liquid, they are usually common substance during formulation, such as day
Right or regenerated minerals, solvent, dispersing agent, wetting agent, adhesive, thickener, adhesive or fertilizer.
These compositions can be only administered comprising active constituent, can also be mixed together with additive (insecticides adjuvant)
It closes and uses.
According to main biology and/or chemico-physical parameter, composition of the invention and its single active constituent can be more
Kind mode is prepared.The example of possible suitable dosage forms has: dispersible oil-suspending agent, soluble liquid (SL), missible oil (EC), wettable
Property pulvis (WP), soluble powder (SP), aqueous emulsion (EW) (such as oil-in-water and water-in-oil emulsion), suspending agent (SC), oil base or
Dispersing agent (OD) in aqueous dispersion agent, such as oil, can be with the granule of oily miscible solution, microparticle absorption particle form
(GR), water-dispersible granules (WDG), water-soluble granular (SG), ULV (ultra-low volume) preparation and WSB (water-soluble pouch) etc..
Dispersible oil-suspending agent (OD) is mixed each components such as dispersing agent, wetting agent, thickener and solvent in the ratio of formula
It closes uniformly, reaction kettle first is added in emulsifier, dispersing agent, wetting agent, stabilizer, decentralized medium, mixes, be uniformly dispersed；At a high speed
Antifreezing agent and thickener are added under shearing condition, by active constituent toVerticilliumdahliaActivitie Activitie S of Relative activator protein and allantoin in zirconium pearl grinding medium
It is sanded under matter, the dispersible oil-suspending agent containing toVerticilliumdahliaActivitie Activitie S of Relative activator protein and allantoin is made.
Wettable powder may be homogenously dispersed in water, and it is in addition to the active ingredient (s, also containing ionically and/or non-ionically table
Face activating agent (wetting agent, dispersing agent), such as polyethoxylated alkylphenol, polyethoxylated fatty alcohols, Polyethoxylated fatty
Amine, fatty alcohol polyglycol ether sulfate, alkyl sulfonate, alkylbenzene sulfonate, sodium lignosulfonate, 2,2 '-dinaphthylmethane -6,
6 '-sodium disulfonates, nekal, oleoyl methyl sodium taurocholate, except diluent or inert substance.It is wettable to prepare
Property pulvis, active constituent is subtly ground, such as with usual means such as hammer-mill, blower mills (blower mill) and empty
Gas jet grinding machine, and at the same time or then being mixed with formulation aid.
Missible oil is by the way that active constituent to be dissolved in organic solvent or ORGANIC SOLVENT MIXTURES and be added one or more
Ionic and/or nonionic surface active agent (emulsifier) and prepare, the solvent is common disclosed solvent, such as hexamethylene
Ketone, dimethylformamide, butanol, dimethylbenzene or high boiling aromatic compounds or hydrocarbon.The example for the emulsifier that can be used has:
Such as calcium dodecyl benzene sulfonate, the calcium salt of alkarylsulphonic acid；Or nonionic emulsifier, such as fatty acid polyethylene glycol ester, alkaryl
Polyglycol ether, fatty alcohol polyglycol ether；Propylene oxide/ethylene oxide condensate；Alkyl, polyether；Sorbitan ester,
Such as sorbitan fatty acid esters, polyoxyethylene sorbitan ester or polyoxyethylene sorbitan fatty acid esters.
Wettable powder is the solid matter fine crushing by prepared by active constituent and solid matter fine crushing grinding
Such as talcum, natural clay (such as kaolin, bentonite and pyrophyllite) or diatomite.
Colloidal suspending agent can be water base or oil base.It for example can add or not add the surfactant having been mentioned above
It is wet by using the progress of commercially available sand mill in the case where (as being had been mentioned in the case where other types of formulation above)
Method is ground and is prepared.
Aqueous emulsion, such as oil in water emulsion (EW) can for example be had been mentioned above using aqueous organic solvent and if appropriate
Surfactant (as being had been mentioned in the case where other types of formulation above) pass through blender, colloid mill and/or static state
It is prepared by mixer.
Granule can be by the way that active constituent to be sprayed on absorbability particle shape inert material or by by adhesive
(such as polyvinyl alcohol, Sodium Polyacrylate or mineral oil) by active constituent concentrating agents be applied to carrier (such as sand, kaolinite) or
The surface of particulate inert material and prepare.The active constituent can also prepare the usual manner granulation of fertiliser granulates, if you need to
It to be mixed with fertilizer.
Water-dispersible granules are usually prepared by a conventional method, and such as pass through spray drying, fluidized bed granulation, disc type system
Grain is mixed with super mixer and squeezes or mediate in the case where not adding solid inert material granulation.
The processing method of suspoemulsion includes that one of those or two effective components are prepared into suspending agent or aqueous emulsion, separately
One or two outer effective component prepares aqueous emulsion or suspending agent, and by suspending agent and aqueous emulsion under the cooperation of auxiliary agent, stirring is equal
It is even, it is prepared into suspension emulsion formulation.
The processing method of soluble concentrate includes that active constituent, surfactant, auxiliary agent and water are placed in reaction vessel,
Under bath temperature control condition, it is sufficiently stirred until obtaining transparency liquid to get soluble concentrate.
The processing method of suspending agent includes putting into effective component toVerticilliumdahliaActivitie Activitie S of Relative activator protein, allantoin and auxiliary agent and water
It is stirred in reaction kettle, after mixing evenly, puts into sand mill after being ground to certain partial size and of the invention hang can be obtained
Floating agent.
The processing method of ultra-low-volume formulation includes after effective component and solvent are stirred dissolution in the reaction vessel, then
Auxiliary component is added, is thoroughly mixed uniformly to get to ultra-low-volume formulation.The method of administration of this dosage form is ultra-low volume spray
Mist, low-gallonage spraying or ultra-low volume electrostatic spray.It is usually sprayed with ultra low volume sprayer, or uses electrostatic atomiser
It is spraying, a small amount of water dilution can also be added to carry out low-gallonage spraying.
In addition, if suitable, auxiliary agent involved in above-mentioned active agent preparation include but do not limit adhesive, wetting agent,
Dispersing agent, emulsifier, bleeding agent, preservative, antifreezing agent, solvent, filler, carrier, colorant, defoaming agent, evaporation suppressor and
PH and viscosity modifier, these reagents are respectively conventional.
Suitable solvent is including but not limited to soybean oil, castor oil, palm oil, gasoline, diesel oil, mineral oil, toluene, two
One or more of toluene, cyclohexanone, methylene chloride, ethyl acetate, chloroform, acetone, hexane.
Suitable surfactant (bleeding agent) is including but not limited to fatty alcohol polyoxyethylene ether, castor oil polyoxyethylene
Ether, alkylphenol-polyethenoxy polyethenoxy ether, phenethyl phenol polyoxyethanyl polyoxyethyl propyl ether, EO-PO block compound, polyoxy second
One or more of emulsifier well known to alkene sorbitan carboxylic esters, alkyl phenol polyoxyethylene ether phosphate etc..Thickener
It is one or more of for xanthan gum, carboxylic first (second) base sodium cellulosate, methylcellulose, polyvinyl alcohol, polyvinylpyrrolidone.It is antifreeze
Agent be ethylene glycol, propylene glycol, glycerine, polyethylene glycol 400 one or more.
Suitable dispersing agent is including but not limited to fragrant race's sulfonate, acrylic acid and acrylic ester copolymers, formaldehyde condensation products
Sulfonate, copolymer-maleic anhydride sodium salt, polymerization of carboxylic acid sodium salt, styrene alkyl polyoxyethylene ether phosphate, sodium naphthalene sulfonate
Formaldehyde condensation products, triphenyl vinyl phenol polyethenoxy ether class and derivative eater, fatty alcohol polyethenoxy ether class, alkyl phenol polyoxy
Ethylene ethers, macromolecule EO-PO block copolymer, lignosulfonates, alkylphenol-polyethenoxy formaldehyde condensation products sulfate, alkane
Base phenol polyethenoxy ether formaldehyde condensation products sulfate phosphates, styrene phenol formaldehyde resin polyoxyethylene ether phosphate salt, benzene
Vinyl phenol polyoxyethylene poly-oxygen propylene aether, alkyl naphthalene formaldehyde condensate sulfonate, macromolecule dredge the branch dress copolymer of type, polymerization
One or more of metal carboxylate is applied in combination.
Suitable emulsifier is including but not limited to anhydrous sorbitol laurate, polyoxyethylene laurel ether, poly alkyl alcohol
One kind of ethylene oxide ether, polyoxyethylene monopalmitate and Emulsifier EL-60.
Suitable filler is including but not limited to modified water-soluble starch, cellulose ether and its derivative, aluminium-magnesium silicate, advanced age
One of soil, diatomite, attapulgite, glucose, precipitated calcium carbonate, white carbon black, inorganic salts.
Suitable wetting agent is including but not limited to lauryl sodium sulfate, Nonyl pheno, alkyl phenol polyoxy second
Cyclohexene carboxaldehyde condensate sulfate, APES formaldehyde condensation products sulfat phosphate, styrene phenol formaldehyde resin
Polyoxyethylene ether phosphate salt, styryl phenol polyethenoxy polyethenoxy ether, alkyl naphthalene formaldehyde condensate sulfonate, alkyl fourth
One of two acid ester sulfonates, this disk SPAN, soil temperature TWEEN two or more are applied in combination.
Suitable adjuvant is including but not limited to binder, disintegrating agent, pH adjusting agent, sterilization antiseptic, defoaming agent, anti-
Freeze the combination of one or more of agent, thickener；Wherein: the binder including but not limited to polyvinyl alcohol, gelatin,
Arabic gum, xanthan gum, Sodium Polyacrylate, sodium alginate, starch, cellulose and its derivates, preferred cellulose and its fiber
One kind of plain derivative；Disintegrating agent choosing is including but not limited to aluminium-magnesium silicate, polyethylene glycol PEG, modified starch, inorganic salts, white
One kind of carbon black, silica gel；The pH acid-base modifier is including but not limited to glacial acetic acid, hydrochloric acid, ammonium hydroxide, ethanol amine, three second
One of hydramine；The sterilization antiseptic is including but not limited to potassium sorbate, sodium benzoate, formaldehyde, isothiazolinone
One of class；The defoaming agent is including but not limited to natural oil, polyethers defoaming agent, higher alcohols, organic silicon defoamer
One kind.The antifreeze is including but not limited to propylene glycol or ethylene glycol；The thickener including but not limited to xanthan gum,
Carragheen, pectin, agar, seaweed glue or sodium carboxymethylcellulose.
According to above-mentioned auxiliary agent, at least can see that
A kind of soluble concentrate composition for regulating crop growth, the effective component of the composition by active component A and
Active component B composition, the active component A is toVerticilliumdahliaActivitie Activitie S of Relative activator protein, and active component B is allantoin, active component A
Weight ratio with active component B is 1:1~300, and auxiliary agent includes at least solvent, dispersing agent, antifreeze, fertilizer or microbial bacteria
Agent, effective component total amount account for entire preparation total amount 1~85%.
A kind of wettable powder composition for regulating crop growth, the effective component of the composition is by active component A
It is formed with active component B, the active component A is toVerticilliumdahliaActivitie Activitie S of Relative activator protein, and active component B is allantoin, active group
The weight ratio of point A and active component B is 1:1~300, auxiliary agent include at least filler, dispersing agent, wetting agent, thickener, fertilizer or
Microbial bacterial agent, effective component total amount account for entire preparation total amount 1~85%.
A kind of water emulsion composition for regulating crop growth, the effective component of the composition is by active component A and work
Property component B composition, the active component A is toVerticilliumdahliaActivitie Activitie S of Relative activator protein, and active component B is allantoin, active component A with
The weight ratio of active component B is 1:1~300, and auxiliary agent includes at least solvent, dispersing agent, emulsifier, antifreeze, fertilizer or micro- life
Object microbial inoculum, effective component total amount account for entire preparation total amount 1~85%.
A kind of suspension agent composition for regulating crop growth, the effective component of the composition is by active component A and work
Property component B composition, the active component A is toVerticilliumdahliaActivitie Activitie S of Relative activator protein, and active component B is allantoin, active component A with
The weight ratio of active component B is 1:1~300, and auxiliary agent includes at least solvent, dispersing agent, wetting agent, thickener, stabilizer, fertilizer
Or microbial bacterial agent, effective component total amount account for entire preparation total amount 1~85%.
Two kinds of effective components show as synergistic effect in composition of the invention, and the activity of the composition is than using individualized
The independent activity of the expected summation of activity and single compound of closing object is more significant.Composition of the invention is shown
Other features are mainly shown as: 1, composition mixture of the invention has obvious synergistic function；2, due to this composition
Two single dose chemical structures it is widely different, the mechanism of action is entirely different, be not present cross resistance, two single doses can be delayed individually to make
With generated resistance problem；3, composition of the invention can good regulating crop growth, improve disease-resistant resistance, promote make
Object health benefit improves crop yield.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that described herein, specific examples are only used to explain the present invention, is not used to limit
The fixed present invention, any modifications, equivalent replacements and improvementsmade within the spirit and principles of the invention, etc. should be included in
Within protection scope of the present invention.
Percentage is weight percentage (folding hundred) in all formulas of following embodiment.The various preparations of the present composition
Processing technology is the prior art, can be varied according to different situations.
One, dosage form prepares embodiment
(1) processing and embodiment of soluble concentrate
By effective component toVerticilliumdahliaActivitie Activitie S of Relative activator protein and allantoin mixture according to a certain percentage, surfactant,
Auxiliary agent and water, are placed in enamel still, under water bath condition, are sufficiently stirred until obtaining transparency liquid to get soluble liquid.
Embodiment 1:10.4% toVerticilliumdahliaActivitie Activitie S of Relative activator protein allantoin soluble concentrate (1:25)
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 0.4%, allantoin 10%, alkylphenol-polyethenoxy formaldehyde condensation products sulfate 8%,
Methyl esters soybean oil 8%, methanol 12%, water complements to 100%.
Embodiment 2:20.1% toVerticilliumdahliaActivitie Activitie S of Relative activator protein allantoin soluble concentrate (1:200)
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 0.1%, allantoin 20%, waxy Bacillus 10% (6,000,000,000 spores/gram), polyoxy
Ethylene sorbitan carboxylic esters 11%, isopropanol 15%, ethylene glycol 8%, water complements to 100%.
Embodiment 3:32.1% toVerticilliumdahliaActivitie Activitie S of Relative activator protein allantoin soluble concentrate (1:320)
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 0.1%, allantoin 32%, styrene phenol formaldehyde resin polyoxyethylene ether phosphate
Salt 15%, isopropanol 12%, turpentine-based vegetable oil 6%, water complements to 100%.
(2) processing and embodiment of wettable powder
By toVerticilliumdahliaActivitie Activitie S of Relative activator protein and allantoin, it is mixed according to the formula with auxiliary agent and filler uniformly, through air-flow
It crushes to get wettable powder.
Embodiment 4:27.6% toVerticilliumdahliaActivitie Activitie S of Relative activator protein allantoin wettable powder (1:275)
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 0.1%, allantoin 27.5%, solution shallow lake bacillus 5% (5,000,000,000 spores/gram), side
Spore bacillus 8% (8,000,000,000 spores/gram), humic acid 10%, polymerization of carboxylic acid sodium salt 7.5%, dialkyl succinylsuccinate sulfonated ester
8.6%, ammonium sulfate 2.8%, xanthan gum 1.6%, attapulgite complements to 100%.
Embodiment 5:33% toVerticilliumdahliaActivitie Activitie S of Relative activator protein allantoin wettable powder (1:10)
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 3%, allantoin 30%, bacillus subtilis 10% (10,000,000,000 spores/gram), it is wooden
Plain sulfonate 9.4%, this disk SPAN 6.3%, aluminium-magnesium silicate 3.5%, plant ash 10%, bentonite 5.4%, white carbon black is supplied
To 100%.
Embodiment 6:75.5% toVerticilliumdahliaActivitie Activitie S of Relative activator protein allantoin wettable powder (1:150)
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 0.5%, allantoin 75%, lauryl sodium sulfate 8%, sodium bicarbonate 1.2%, fruit
Glue 1.1%, white carbon black complements to 100%.
(3) processing and embodiment of aqueous emulsion
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein and allantoin and solvent, emulsifier, dispersing agent, antifreeze etc. is sufficiently mixed in proportion
It closes, adds to be uniformly mixed after water and can be prepared by aqueous emulsion.
Embodiment 7:6% toVerticilliumdahliaActivitie Activitie S of Relative activator protein allantoin aqueous emulsion (1:1)
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 3%, allantoin 3%, alkyl phenol polyoxyethylene ether phosphate 8%, polyethylene glycol
12%, benzene sulfonic acid sodium salt 4%, bentonite 2.3%, water complements to 100%.
Embodiment 8:35.7% toVerticilliumdahliaActivitie Activitie S of Relative activator protein allantoin aqueous emulsion (1:50)
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 0.7%, allantoin 35%, anhydrous sorbitol laurate 9.8%, lignin sulfonic acid
Salt 10.4%, glycerine 15%, ammonium sulfate 5.6%, water complements to 100%.
Embodiment 9:50.2% toVerticilliumdahliaActivitie Activitie S of Relative activator protein allantoin aqueous emulsion (1:250)
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 0.2%, allantoin 50%, Emulsifier EL-60 6.5%, copolymer-maleic anhydride
Sodium salt 7.9%, ethylene glycol 12%, aluminium chloride 4.6%, water complements to 100%.
(4) processing and embodiment of suspending agent
By each components such as active constituent toVerticilliumdahliaActivitie Activitie S of Relative activator protein and allantoin, dispersing agent, wetting agent, thickener and water
It is mixed according to the formula uniformly, after sand milling and/or high speed shear, obtains semi-finished product, water supplement is uniformly mixed filtering after analysis
It gets product.
Embodiment 10:3.3% toVerticilliumdahliaActivitie Activitie S of Relative activator protein allantoin suspending agent (10:1)
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 3%, allantoin 0.3%, toluene 10%, aromatic sulphonate 6.3%, polyacrylic acid
Sodium 2.7%, sodium bicarbonate 2.1%, alkyl naphthalene formaldehyde condensate sulfonate 5.5%, higher alcohols 0.3%, water complements to 100%.
Embodiment 11:40.4% toVerticilliumdahliaActivitie Activitie S of Relative activator protein allantoin suspending agent (1:100)
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 0.4%, allantoin 40%, mineral oil 12%, lignosulfonates 6%, magnesium silicate
Aluminium 4.8%, sodium chloride 3.3%, lauryl sodium sulfate 9%, organic silicon defoamer 0.6%, water complements to 100%.
Embodiment 12:27.09% toVerticilliumdahliaActivitie Activitie S of Relative activator protein allantoin suspending agent (1:300)
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 0.09%, allantoin 27%, cyclohexanone 15%, alkylphenol-polyethenoxy formaldehyde condensation
Object sulfate 7.9%, xanthan gum 3.6%, ammonium sulfate 3.8%, calcium dodecyl benzene sulfonate 5%, organic silicon defoamer 0.5%,
Water complements to 100%.
Two, drug effect verification test
Test is located in the area Yancheng City great Feng, Jiangsu Province good harvest the earth modern agriculture Technology Park demonstration greenhouse and carries out, and 2018
No. 5 sowings in 2 months, field planting on March 6th, 2018, June~August harvesting.It is as follows for the Main Nutrients of examination plastic shed soil: organic matter
61.26g/kg, full nitrogen 3.01g/kg, full potassium 5.21g/kg, available phosphorus 689mg/kg, available potassium 569mg/kg.For trying cucurbita pepo
Kind is that double Feng Te are early.
On April 3rd, 2018, each foliar spray application on April 16 are primary.Test sets 13 processing, i.e. CK (control, equivalent
Clear water), embodiment 1, embodiment 2, embodiment 3, embodiment 4, embodiment 5, embodiment 6, embodiment 7, embodiment 8, embodiment
9, embodiment 10, embodiment 11, embodiment 12.It is planted using duplicate rows, line-spacing 0.6m, spacing in the rows 0.5m, 9 plants/row, 36 plants/cell,
Plantation density is 2000 plants/acre.3 repetitions are arranged in each processing, are arranged using completely random.About 0.6m wide is stayed between each processing
Isolation strip.Sowing, field management routinely operate progress.
Picking time is sampled, and each cell takes 10 plants to be used as sample at random.Each embodiment is measured to plant growth
Influence, including to crop plant height, yield, Vc content, Soluble adhesion molecule and integrated situations such as controlling powdery mildew etc.
Evaluation (Vc content uses molybdenum blue method, and Soluble adhesion molecule measurement uses anthrone colorimetry).Plant height, yield, Vc in this test
The indexs such as content, Soluble adhesion molecule and powdery mildew disease index are the average value of each processing.
Grade scale:
0 grade: disease-free spot；
1 grade: leaf spot lesion is less than 5, and length is less than 1cm；
3 grades: leaf spot lesion 6-10, part scab length is greater than 1cm；
5 grades: leaf spot lesion 11-25, part scab is linked to be piece, and lesion area accounts for the 10-25% of leaf area；
7 grades: leaf spot lesion 26 or more, scab is linked to be piece, and lesion area accounts for the 26-50% of leaf area；
9 grades: scab is linked to be piece, lesion area account for 50% of leaf area or more or full leaf it is withered.
Plant height control rate=(plant height of plant after plant height-processing of clear water adjoining tree)/clear water adjoining tree plant height *
100%
Per mu yield increase rate=(treated per mu yield-clear water control per mu yield)/clear water control per mu yield * 100%
Vc content increase rate=(treated Vc content-clear water control Vc content)/clear water compares Vc content * 100%
Soluble adhesion molecule increase rate=(treated, and Soluble adhesion molecule-clear water control total Soluble Sugar contains
Amount)/clear water control Soluble adhesion molecule * 100%
Powdery mildew prevention rate=(clear water compares powdery mildew disease index after powdery mildew disease index-processing)/clear water control
Powdery mildew disease index * 100%
1 experimental data of table of subordinate list shows, product of the invention is respectively administered 1 time in cucurbita pepo florescence and phase of bearing fruit, especially
It in 1:1~300 ranges, significantly can effectively adjust the strong of crop in the proportion of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and allantoin
Kang Shengchang.1, it can effectively improve the Soluble adhesion molecule of crop and improve the ascorbic content of crop, article is made in raising
Matter；2, allantoin can effectively prevent Pumpkin powdery mildew, and toVerticilliumdahliaActivitie Activitie S of Relative activator protein has centainly cucurbita pepo growth
Disease-resistant anti-adversity ability has certain synergistic function after the two is mixed；3, composition of the invention produces the unit of crop
Measurer is obviously improved effect.
Claims (9)
1. a kind of composition, which is characterized in that the composition includes two kinds of active components of A and B, and wherein active component A is superfine
Rod method activator protein, active component B are allantoin, and the weight ratio of active component A and active component B are 1:1~300.
2. composition according to claim 1, which is characterized in that active component A and the weight ratio of active component B are 1:5
~275.
3. composition according to claim 1 or 2, which is characterized in that contain one or more in the composition
Microbial bacterial agent.
4. composition according to claim 3, which is characterized in that the microbial bacterial agent is bacillus quasi-microorganism
Microbial inoculum；The bacillus category microbial bacterial agent is preferably bacillus subtilis, bacillus licheniformis, Su Yunjin gemma bar
Bacterium, waxy Bacillus, solution one of shallow lake bacillus and bacillus laterosporus or more than one mixed microorganism strain.
5. compound fertilizer according to claim 3 or 4, which is characterized in that the living bacteria count of the microbial bacterial agent is total
Amount be greater than 200,000,000/gram；Preferably greater than 1,000,000,000/gram.
6. according to claim 1, composition described in 2,3,4 or 5, which is characterized in that the composition is by active constituent and auxiliary agent
The dosage form allowed on pesticide or fertilizer is made.
7. composition according to claim 6, which is characterized in that the dosage form is soluble concentrate, wettable powder, water and milk
Agent, suspending agent, granule, water dispersible granules or aqua.
8. composition according to claim 4, which is characterized in that the insecticides adjuvant be selected from carrier, solvent, dispersing agent,
One or more of wetting agent, adhesive, thickener, adhesive, surfactant or fertilizer.
9. purposes of the composition described in any one of claim 1~8 in agriculture field promotion or coordinate plant growth.
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| CN201910252379.8A CN109964960A (en) | 2019-03-29 | 2019-03-29 | Composition containing toVerticilliumdahliaActivitie Activitie S of Relative activator protein and allantoin |
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Cited By (1)
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| CN115868500A (en) * | 2022-12-23 | 2023-03-31 | 东北农业大学 | Inhibitor for antagonizing powdery mildew of melons and preparation method and application thereof |
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