CN111165514A

CN111165514A - Composition with synergistic effect

Info

Publication number: CN111165514A
Application number: CN202010018117.8A
Authority: CN
Inventors: 仲汉根; 季红进; 花伟
Original assignee: Jiangsu Huifeng Agrochemical Co Ltd
Current assignee: Jiangsu Huifeng Agrochemical Co Ltd
Priority date: 2020-01-08
Filing date: 2020-01-08
Publication date: 2020-05-19

Abstract

The invention discloses a composition with a synergistic effect, which comprises two active components A and B, wherein the active component A is an alternaria tenuissima activator protein, and the active component B is phosphite or a derivative thereof; the weight ratio of the active component A to the active component B is 1:2 to 45. The invention also provides a preparation method and application of the composition. The test result shows that the composition of the invention can well regulate the growth of crops, improve the resistance to diseases and stress, promote the health of the crops and improve the yield of the crops.

Description

Composition with synergistic effect

Technical Field

The invention belongs to the field of agricultural plant protection, and particularly relates to a pesticide composition with improved performance, in particular to a composition containing alternaria tenuissima activator protein and phosphite.

Background

The alternaria tenuissima activator protein (plant activator protein) is a novel protein screened, separated and purified from alternaria alternata, and the plant autoimmunity is improved mainly by activating a molecular immune system in a plant body; by exciting a series of metabolism regulation and control in the plant body, the growth of plant roots, stems and leaves is promoted, the chlorophyll content is increased, the crop yield is increased, and the plant growth regulator has a high prevention effect on various plant diseases.

The phosphite is proved to have the effects of increasing the yield and resisting diseases for crops, and is a novel environment-friendly chemical preparation. Phosphite is widely applied to the field of plant disease and insect pest control and plant nutrition at home and abroad at present. The phosphite has good effect on inhibiting oomycete germs of solanum, brassica and other leafy vegetables and the like when being used as a bactericide, and has good fertilizer effect on increasing flowering, yield, fruit size, soluble substance content and the like of crops such as strawberries, potatoes and the like. The action mechanism is that phosphorous acid phosphorus can be transported in the xylem and phloem of crops in double channels, so that the absorption and utilization of nutrients are accelerated; in the aspect of disease resistance, researchers generally believe that phosphorous acid phosphorus can induce crops to generate defense toxin and disease course related protein (PR protein), possibly rely on Salicylic Acid (SA) to open a defense mechanism, and enable the crops to generate continuous immunity to pathogenic bacteria. The potassium phosphite is a novel product which is prepared by scientifically proportioning phosphite and organic active potassium which are special raw materials through high-temperature reaction, the potassium phosphite is not only a phosphorus-potassium fertilizer with 3-valent phosphorus, but also a systemic bactericide, the potassium phosphite can not generate resistance after repeated use, and the utilization rate and the absorption rate of the potassium phosphite are more than 2 times of those of similar products.

The plant growth regulator sold on the market at present mainly takes a single agent as a main agent, has a single action effect, has great risk on safety of compounding common plant growth regulators, and is easy to cause crop growth deformity, seedling runt, fruit drop, yield reduction and quality reduction.

Disclosure of Invention

The invention aims to provide a composition which can synergistically promote the growth of crops, improve the yield of the crops and has certain disease resistance. The inventor finds that the alternaria tenuissima activator protein and the phosphite have obvious synergistic effect in the aspects of regulating and promoting plant growth and preventing and controlling crop diseases.

The object of the invention can be achieved by the following measures:

a composition comprises two active components A and B, wherein the active component A is alternaria tenuissima activator protein, the active component B is phosphite or a derivative thereof, and the active component B is preferably potassium phosphite, sodium phosphite, calcium phosphite or choline phosphite, but not limited thereto; further preferred is potassium phosphite or choline phosphite. The weight ratio of the active component A to the active component B is 1:2 to 45.

In general, the potassium phosphite used in the present invention may be potassium dihydrogen phosphite or dipotassium hydrogen phosphite, or a mixture of potassium dihydrogen phosphite and dipotassium hydrogen phosphite, which are collectively referred to as potassium phosphite. Similarly, the sodium salt of phosphorous acid is sodium dihydrogen phosphite or disodium hydrogen phosphite, or a mixture of sodium dihydrogen phosphite and disodium hydrogen phosphite, which are collectively called sodium salt of phosphorous acid.

The composition can be prepared into a pesticide allowable dosage form by an active ingredient and a pesticide auxiliary agent. Furthermore, the composition comprises 1-85% of active components and 99-15% of pesticide auxiliary agents by weight, and forms a pesticide allowable dosage form.

The inventor finds out through experiments that the composition provided by the invention is used for better regulating the growth of crops, controlling diseases of the crops and improving the yield of the crops.

The invention provides application of a composition containing a component A (alternaria tenuissima activator protein) and a component B (phosphite or a derivative thereof) in the aspects of regulating (promoting) crop growth and preventing and controlling crop diseases in the agricultural field, in particular application in the aspects of promoting rice growth and improving disease resistance.

In order to fully exert the advantages and effects of the invention, the composition of the invention can also be mixed with other pesticides (such as bactericides, insecticides, herbicides, plant growth regulators), fertilizers and the like, and the mixture is common pesticides or fertilizers disclosed by the prior art.

The composition may specifically contain pesticide adjuvants, such as one or more of carrier, solvent, dispersant, wetting agent, adhesive, thickener, binder, surfactant, etc., and the conventional adjuvants may be mixed during application.

Suitable auxiliaries may be solids or liquids, which are generally substances customary in the processing of dosage forms, such as natural or regenerated mineral substances, solvents, dispersants, wetting agents, adhesives, thickeners, binders or fertilizers.

These compositions may be applied by containing only the active ingredient, or may be used in admixture with additives (pesticidal adjuvants).

The compositions of the invention and their individual active ingredients can be formulated in various ways, according to the prevailing biological and/or chemical-physical parameters. Examples of possible suitable dosage forms are: aqueous, dispersible oil suspensions, soluble Solutions (SL), Emulsifiable Concentrates (EC), Wettable Powders (WP), Soluble Powders (SP), aqueous Emulsions (EW) (such as oil-in-water and water-in-oil emulsions), Suspension Concentrates (SC), oil-based or water-based dispersants, for example dispersants in Oil (OD), oil-miscible solutions, granules in the form of microparticulate adsorbent particles (GR), water-dispersible granules (WDG), water-soluble particles (SG), ULV (ultra low volume) formulations and WSB (water-soluble sachets).

Dispersible oil suspension (OD) uniformly mixes the components of dispersant, wetting agent, thickener and solvent according to the proportion of the formula, firstly adds the emulsifier, dispersant, wetting agent, stabilizer and dispersion medium into a reaction kettle, and uniformly mixes and disperses; adding an antifreezing agent and a thickening agent under a high-speed shearing state, and sanding active ingredients of the alternaria tenuissima activator protein and the phosphite or the derivative thereof in a zirconium bead grinding medium to prepare the dispersible oil suspending agent containing the alternaria tenuissima activator protein and the phosphite or the derivative thereof.

Wettable powders are homogeneously dispersible in water and contain, in addition to the active ingredient, ionic and/or nonionic surfactants (wetting agents, dispersants), for example polyethoxylated alkylphenols, polyethoxylated fatty alcohols, polyethoxylated fatty amines, fatty alcohol polyglycol ether sulfates, alkanesulfonates, alkylbenzenesulfonates, sodium lignosulfonates, sodium 2, 2 '-dinaphthylmethane-6, 6' -disulfonate, sodium dibutylnaphthalenesulfonate, sodium oleoyl methyltaurate, with the exception of diluents or inerts. To prepare wettable powders, the active ingredient is finely ground, for example with customary devices such as hammer mills, blast mills (blower mills) and air-jet mills, and mixed simultaneously or subsequently with the formulation auxiliaries.

Wettable powders are prepared by grinding the active ingredient with a finely divided solid substance, for example talc, natural clays (such as kaolin, bentonite and pyrophyllite) or diatomaceous earth.

The suspension concentrates may be water-based or oil-based. It can be prepared, for example, by wet grinding using a commercially available sand mill, with or without the addition of the surfactants already mentioned above (as already mentioned above in the case of other dosage form types).

Aqueous emulsions, for example oil-in-water Emulsions (EW), can be prepared, for example, using aqueous organic solvents and, if appropriate, the surfactants already mentioned above (as already mentioned above in the case of the other dosage form types), by means of stirrers, colloid mills and/or static mixers.

Granules can be prepared by spraying the active ingredient onto an absorbent particulate inert material or by applying a concentrate of the active ingredient to a carrier (e.g. sand, kaolinite) or to the surface of a particulate inert material by means of a binder (e.g. polyvinyl alcohol, sodium polyacrylate or mineral oil). The active ingredient may also be granulated in the conventional manner for the preparation of fertilizer granules, if desired mixed with fertilizers.

Water-dispersible granules are generally prepared by customary methods, such as by spray drying, fluidized-bed granulation, pan granulation, mixing with high-speed mixers, and extrusion or kneading granulation without addition of solid inert materials.

The processing method of the suspoemulsion comprises the steps of preparing one or two effective components into a suspending agent or an aqueous emulsion, preparing the other one or two effective components into the aqueous emulsion or the suspending agent, and uniformly stirring the suspending agent and the aqueous emulsion under the coordination of an auxiliary agent to prepare the suspoemulsion.

The processing method of the soluble solution comprises placing active ingredients, surfactant, adjuvant and water in a reaction vessel, and stirring thoroughly under the condition of water bath temperature control until a transparent liquid is obtained to obtain the soluble solution.

The processing method of the suspending agent comprises the steps of putting the active ingredients of the alternaria tenuissima activator protein, the phosphite or the derivative thereof, the auxiliary agent and water into a reaction kettle for stirring, putting the mixture into a sand mill for grinding the mixture to a certain particle size after the mixture is stirred uniformly, and obtaining the suspending agent.

The processing method of the ultra-low volume agent comprises the steps of stirring and dissolving the effective components and the solvent in a reaction container, then adding the auxiliary components, fully stirring and uniformly mixing to obtain the ultra-low volume agent. The application method of the preparation is ultra-low volume spraying, low volume spraying or ultra-low volume electrostatic spraying. The spray is usually carried out by an ultra-low volume sprayer, or by an electrostatic sprayer, or by low volume spraying by diluting with a small amount of water.

Furthermore, the auxiliaries mentioned above in connection with the active ingredient formulations include, if appropriate, without limitation, binders, wetting agents, dispersants, emulsifiers, penetrants, preservatives, antifreezes, solvents, fillers, carriers, colorants, antifoams, evaporation inhibitors, and pH and viscosity regulators, each of which is conventional.

Suitable solvents include, but are not limited to, one or more of soybean oil, castor oil, palm oil, gasoline, diesel oil, mineral oil, toluene, xylene, cyclohexanone, methylene chloride, ethyl acetate, chloroform, acetone, hexane.

Suitable surfactants (penetrants) include, but are not limited to, fatty alcohol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol polyoxyethylene polyoxypropylene ethers, phenethylphenol polyoxyethylene polyoxypropylene ethers, EO-PO block compounds, polyoxyethylene sorbitan fatty acid esters, alkylphenol polyoxyethylene phosphate esters, and one or more of the known emulsifiers. The thickener is one or more of xanthan gum, sodium carboxymethyl (ethyl) cellulose, methylcellulose, polyvinyl alcohol and polyvinylpyrrolidone. The antifreezing agent is one or more of ethylene glycol, propylene glycol, glycerol and polyethylene glycol 400.

Suitable dispersing agents include, but are not limited to, aromatic sulfonates, acrylic acid-acrylic ester copolymers, formaldehyde condensate sulfonates, maleic anhydride copolymer sodium salts, polymeric sodium carboxylates, styrene alkyl polyoxyethylene ether phosphate esters, sodium naphthalene sulfonate formaldehyde condensates, tristyrylphenol polyoxyethylene ethers and derivative esters, fatty alcohol polyoxyethylene ethers, alkylphenol polyoxyethylene ethers, one or more of macromolecule EO-PO block copolymer, lignosulfonate, alkylphenol polyoxyethylene formaldehyde condensate sulfate phosphate, styrene phenol formaldehyde resin polyoxyethylene ether phosphate, styrene phenol polyoxyethylene polyoxypropylene ether, alkyl naphthalene formaldehyde condensate sulfonate, macromolecule hydrophobic type branch-mounted copolymer and polymeric carboxylate are used in combination.

Suitable emulsifiers include, but are not limited to, one of sorbitan laurate, polyoxyethylene lauryl ether, fatty alcohol polyoxyethylene ether, polyoxyethylene monopalmitate, and polyoxyethylene castor oil.

Suitable fillers include, but are not limited to, one of modified water soluble starch, cellulose ether and its derivatives, magnesium aluminum silicate, kaolin, diatomaceous earth, attapulgite, glucose, precipitated calcium carbonate, white carbon, inorganic salts.

Suitable wetting agents include, but are not limited to, sodium lauryl sulfate, nonylphenol polyoxyethylene, alkylphenol polyoxyethylene formaldehyde condensate sulfate phosphate, styrene phenol formaldehyde resin polyoxyethylene ether phosphate, styrene phenol polyoxyethylene polyoxypropylene ether, alkyl naphthalene formaldehyde condensate sulfonate, alkyl succinate sulfonate, SPAN, TWEEN, or combinations of two or more thereof.

Suitable adjuvants include, but are not limited to, one or a combination of several of binders, disintegrants, pH adjusting agents, antimicrobial preservatives, antifoaming agents, anti-freezing agents, and thickeners; wherein: the binder comprises but is not limited to polyvinyl alcohol, gelatin, Arabic gum, xanthan gum, sodium polyacrylate, sodium alginate, starch, cellulose and derivatives thereof, and is preferably one of cellulose and cellulose derivatives thereof; the disintegrating agent is selected from one of magnesium aluminum silicate, polyethylene glycol (PEG), modified starch, inorganic salt, white carbon black and silica gel; the pH acid-base regulator comprises but is not limited to one of glacial acetic acid, hydrochloric acid, ammonia water, ethanolamine and triethanolamine; the sterilizing preservative comprises but is not limited to one of potassium sorbate, sodium benzoate, formaldehyde and isothiazolinone; the defoaming agent comprises one of natural grease, polyether defoaming agent, higher alcohol and organic silicon defoaming agent. The antifreeze agent includes, but is not limited to, propylene glycol or ethylene glycol; the thickener includes, but is not limited to, xanthan gum, carrageenan, pectin, agar, alginate jelly, or sodium carboxymethylcellulose.

From the above auxiliaries, at least:

a composition in the form of wettable powder comprises active component A and active component B, wherein the active component A is alternaria tenuissima activator protein, the active component B is phosphite or choline phosphite, and the weight ratio of the active component A to the active component B is 1: 1-50 percent of additive, wherein the additive at least comprises filler, dispersant, wetting agent and thickening agent, and the total amount of active ingredients accounts for 1-85 percent of the total amount of the whole preparation.

A composition in the form of dispersible oil suspension agent comprises active component A and active component B, wherein the active component A is alternaria tenuissima activator protein, the active component B is phosphite or choline phosphite, and the weight ratio of the active component A to the active component B is 1: 1-50 percent of auxiliary agent, wherein the auxiliary agent at least comprises solvent, dispersant, wetting agent, thickening agent and stabilizer, and the total amount of the effective components accounts for 1-85 percent of the total amount of the whole preparation.

A composition in the form of suspending agent is prepared from active component A and active component B, the active component A is Alternaria tenuissima activator protein, the active component B is phosphite or choline phosphite, and the weight ratio of the active component A to the active component B is 1: 1-50 percent of auxiliary agent, wherein the auxiliary agent at least comprises solvent, dispersant, wetting agent, thickening agent and stabilizer, and the total amount of the effective components accounts for 1-85 percent of the total amount of the whole preparation.

The two active principles of the composition according to the invention show a synergistic effect, the activity of which is more pronounced than the expected sum of the activities of the individual compounds used, and the individual activities of the individual compounds. The compositions of the invention exhibit other characteristics which are mainly characterized in that: 1. the composition has obvious synergistic effect when mixed; 2. because the two single agents of the composition have great difference in chemical structure and completely different action mechanisms, the composition can play a role in synergy and complementation; 3. the composition of the invention can well regulate the growth of crops, improve the resistance to diseases and stress, promote the health of the crops and improve the yield of the crops.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the embodiments described herein are merely illustrative of the present invention and are not intended to limit the invention, and any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the scope of the present invention.

The percentages in all formulations in the following examples are in weight percent (in percent). The processing technology of various preparations of the composition is the prior art, and can be changed according to different conditions.

First, preparation examples of dosage forms

(I) processing of wettable powder and its embodiment

Mixing the alternaria tenuissima activator protein, phosphite or choline phosphite, an auxiliary agent and a filler uniformly according to the proportion of the formula, and performing air flow crushing to obtain the wettable powder.

Example 1: 72% wettable powder of Alternaria tenuissima activator protein sodium phosphite (1: 15)

4.5 percent of Alternaria tenuissima activator protein, 67.5 percent of sodium phosphite, 5 percent of alkyl naphthalene formaldehyde condensate sulfonate, 1.2 percent of monopotassium phosphate, 0.8 percent of magnesium aluminum silicate and kaolin which are complemented to 100 percent.

Example 2: 55.2% wettable powder of Alternaria tenuissima activator protein and potassium phosphite (1: 45)

1.2 percent of alternaria tenuissima activator protein, 54 percent of potassium phosphite, 6.2 percent of formaldehyde condensate sulfonate, 5.4 percent of alkylphenol polyoxyethylene formaldehyde condensate sulfate, 0.5 percent of gelatin, 0.7 percent of silica gel and the balance of white carbon black to 100 percent.

Processing and examples of dispersible oil suspensions

Mixing surfactant and oil base, adding active components such as alternaria tenuissima activator protein and phosphite (or choline phosphite) under high shear stirring, grinding in sand mill until the particle size is in a certain range.

Example 3 dispersible oil suspension of 21% Alternaria tenuissima activator protein Choline Phosphite (1: 2)

7% of Alternaria tenuissima activator protein, 14% of choline phosphite, 3.8% of aromatic sulfonate, 7.2% of sodium dodecyl sulfate, 2.7% of Arabic gum, 3.2% of cellulose, 12% of methyl soybean oil and the balance of vegetable oil to 100%.

Example 4 33.8% Alternaria tenuissima activator protein potassium phosphite dispersible oil suspension (1: 25)

1.3 percent of Alternaria tenuissima activator protein, 32.5 percent of potassium phosphite, 5.8 percent of fatty alcohol-polyoxyethylene ether, 8.2 percent of alkyl succinate sulfonate, 6 percent of butyl hydroxy anisole, 1.8 percent of magnesium aluminum silicate, 10 percent of cyclohexane and the balance of methyl ester and soybean oil to 100 percent.

(III) processing of the suspending agent and examples

Uniformly mixing the active ingredient of the alternaria tenuissima activator protein with phosphite, a dispersing agent, a wetting agent, a thickening agent, water and the like according to the proportion of the formula, sanding and/or shearing at high speed to obtain a semi-finished product, adding water after analysis, uniformly mixing and filtering to obtain a finished product.

Example 5: 15% Alternaria tenuissima activator protein potassium phosphite suspending agent (1: 1)

7.5 percent of alternaria tenuissima activator protein, 7.5 percent of potassium phosphite, 8 percent of lignosulfonate, 8 percent of styryl phenol polyoxyethylene polyoxypropylene ether, 5 percent of polyvinyl alcohol, 1.1 percent of magnesium aluminum silicate, 12 percent of propylene glycol, 0.1 percent of higher alcohol and water which are complemented to 100 percent.

Example 6: 43.2% Alternaria tenuissima activator protein calcium phosphite suspending agent (1: 35)

1.2% of alternaria tenuissima activator protein, 42% of calcium phosphite, 10% of cyclohexane, 5.4% of polymeric sodium carboxylate, 2.5% of xanthan gum, 1.5% of sodium alginate, 6.8% of sodium dodecyl sulfate, 5% of ethylene glycol, 0.8% of organic silicon defoamer and water in balance to 100%.

Example 7: 25.5% Alternaria tenuissima activator protein choline phosphite suspending agent (1: 50)

0.5% of alternaria tenuissima activator protein, 25% of choline phosphite, 8% of methanol, 6.4% of lignosulfonate, 3.2% of polyethylene glycol, 7.8% of alkyl succinate sulfonate, 8% of glycerol, 0.5% of higher alcohol and the balance of water to 100%.

Second, efficacy test

1. Environmental and facility culture conditions

1.1 test site location

Shandong province, Jinan City, ancient city, mountain town, Suzhou village, ancient city, China.

1.2 test crops, varieties and growth conditions

The rice is dry rice in Israel, and is sowed in 2018 in 6 months and 12 days, wherein the sowing quantity per mu is 6kg, the row spacing is 25cm, and the furrow width is 5 m.

1.3 soil type of test plot

The test field is loam, the hydrolyzable nitrogen is 94.64mg/kg, the available phosphorus is 62.73mg/kg, the quick-acting potassium is 384mg/kg, the Organic Matter (OM) is 25.94g/kg, the pH value is 8.13, and the water-soluble salt is 2.42 g/kg.

1.4 Water and Fertilizer management of test fields

The test terrain is flat, the management is consistent, the fertility and the water pouring conditions are better, and the rice is watered once in 6 months and 12 days in 2018 after the rice is sown. Rain exists in 25 days 6 and 26 days 6 and 27 days 6 and 8 in 2018, and artificial watering is not performed in the later growth stage of the test field.

Wheat is planted in the previous crop of the test field, and the herbicide is pinoxaden and clodinafop-propargyl.

1.5 information of chemical for preventing and controlling other diseases, pests and weeds

No other plant growth regulator was applied throughout the test period; the fenoxaprop-p-ethyl and fluroxypyr are sprayed on the whole field in 2018, 6 months and 21 days to control weeds in the test field, and other plant regulators are not applied in the whole test period. In addition, abamectin and imidacloprid are applied for preventing and controlling rice planthopper for 1 time in the tillering stage of rice, and the insecticidal composition is used for preventing and controlling chilo suppressalis and rice leaf roller.

2. Design and arrangement of experiments

2.1 dosage and numbering of drugs

TABLE 1 test design of test agents

2.2 cell arrangement

1	2	3	4	5	6
						7	8	9	10	11	12
13	14	15	16	17	18
						19	20	21	22	3	7
9	2	1	8	6	11
						15	13	5	10	4	14
17	16	12	18	20	21
						19	22	5	2	19	9
10	4	12	1	16	13
						7	15	3	11	6	17
18	22	8	20	14	21

2.3 cell area and repetition

Cell area: 20 square meters;

the number of repetitions: 3 times.

2.4 methods of application

2.4.1 time period of use and method

Spraying the pesticide 1 times respectively in tillering stage and booting heading stage of rice, and applying the pesticide 2 times in total.

2.4.2 applicator devices

The spraying device is a superior knapsack sprayer.

2.4.3 application time and frequency

The test was performed on 19 days 7 and 5 days 9 and 5 days 2018, respectively, and 2 times of administration were performed. The rice is in the tillering stage and the booting heading stage respectively, and the growth vigor is good.

2.4.4 Capacity of use

The dosage of the medicament is shown in table 1.

3. Investigation method, time and number of times

3.1 investigation time and number of surveys

Recording whether the rice has phytotoxicity (symptoms such as withering, chlorosis, deformity and the like) by a visual inspection 7 days after each pesticide application; investigating plant setting percentage, disease prevention or control condition (mainly rice blast), yield and other indexes in the rice harvesting period; a total of two investigations were carried out.

3.2 investigation method

And (3) measuring the number of effective ears of rice when the rice is mature, sampling 5 points per cell, taking 20 plants per point, randomly taking 10 plants to measure the grain number (number of grains per ear), the occurrence condition of diseases and other yield indexes, and actually collecting and measuring yield of each cell.

Setting percentage is 100% of the number of grains per ear/total number of grains per ear

The control effect is (disease index after drug administration in blank control area-disease index after drug administration in drug treatment area)/disease index after drug administration in blank control area is 100%

The yield per mu is increased to 100 percent (the processed yield per mu-the yield per mu of the clear water contrast)/the yield per mu of the clear water contrast

Grading standard:

level 0: the whole plant is disease-free;

level 1: the fourth leaf and the leaf sheaths and leaves below the fourth leaf (sword leaf is taken as the first leaf);

and 3, level: the third leaf and the leaf sheaths and leaves below the third leaf are attacked;

and 5, stage: the second leaf and the leaf sheaths and leaves below the second leaf are attacked;

and 7, stage: attack of Jianye leaf and the leaf sheaths and leaves below the Jianye leaf;

and 9, stage: the whole plant is attacked and withered in advance.

3.3 direct effects on crops

The test reagent has no adverse effect on the growth of rice, and no obvious phytotoxicity spots are seen on leaves.

3.4 quality and yield of the product

And (5) harvesting the yield.

3.5 Effect on other organisms

3.5.1 Effect on other pests

No investigation was made.

3.5.2 Effect on other non-target organisms

Not investigated

4. Results and analysis

4.1 safety

The test agent is detected and investigated 7 days after the second application, which has no adverse effect on the growth of rice and no obvious phytotoxicity spots on leaves.

4.2 analysis

TABLE 2 comprehensive test results of the combination of Alternaria tenuissima activator protein and phosphite for regulating the growth of rice

5. Conclusion

In the rice harvesting period (harvesting in 25 days in 10 months, see table 2), test results show that, in combination with various growth determination indexes of rice, under the test proportion and dosage, the compound use of the alternaria tenuissima activator protein and phosphite is 1: 1-1: 50 relative to the single dose, certain difference exists in effect, and the test medicament alternaria tenuissima activator protein: phosphite 1: 2-45, the composition has a good synergistic effect, wherein the alternaria tenuissima activator protein: phosphite has the best effect when the ratio of phosphite is 1:25, and the setting rate is 99.58; the prevention effect on rice blast is the best, and the prevention effect is 86.10%; the yield increase rate is highest and is 17.77%. Its next superfine alternaria activator protein: phosphite 1:2, alternaria tenuissima activator protein: phosphite 1:15, alternaria tenuissima activator protein: phosphite 1:35, alternaria tenuissima activator protein: phosphite is also preferred as 1:45, with setting percentages of 98.47%, 99.04%, 99.12%, 98.41%, respectively; the preventive effects on rice blast are 79.54%, 82.97%, 83.44% and 80.25%, respectively; the yield increase rate is 14.64%, 15.98%, 16.16% and 14.22% respectively. And the alternaria tenuissima activator protein: phosphite 1:1 and 1: in the mixture ratio interval of 50, the main growth indexes of the rice show additive effect and no obvious effect.

Alternaria tenuissima activator protein: 1 part of phosphite: preferably 2 to 45. The pesticide is continuously applied for 2-3 times, has good effects on setting percentage, rice blast blight prevention effect and yield increase rate, has no adverse effect on growth and development of rice, and has good safety.

The application is suitable for the period: and (4) continuously applying the pesticide for 2-3 times at the tillering stage and the heading stage of the rice.

The application method comprises the following steps: spraying stem and leaf.

Safety: the compounding of the alternaria tenuissima activator protein and the phosphite is safe for rice, the growth and development of the rice are normal after the application, and no adverse reaction is seen.

Claims

1. A composition having a synergistic effect, characterized in that: the composition comprises two active components A and B, wherein the active component A is an alternaria tenuissima activator protein, and the active component B is phosphite or a derivative thereof.

2. The composition of claim 1, wherein: the active component B is potassium phosphite, phosphorous acid, calcium sodium phosphite or choline phosphite; the active component B is preferably potassium phosphite or choline phosphite.

3. The composition of claim 1, wherein: the weight ratio of the active component A to the active component B is 1:2 to 45.

4. The composition of claim 1, wherein: the composition is prepared into a pesticide allowable dosage form by active ingredients and auxiliaries.

5. The composition of claim 4, wherein: the preparation is wettable powder, suspending agent, dispersible oil suspending agent or aqueous agent.

6. The composition of claim 4, wherein: the auxiliary agent is selected from one or more of a carrier, a solvent, a dispersing agent, a wetting agent, an adhesive, a thickening agent, a binding agent, a surfactant or a fertilizer.

7. Use of the composition of any one of claims 1 to 6 for controlling crop diseases and promoting crop growth in the agricultural field.