CN113875778A - Biostimulant comprising plant immunoactive protein and 5-aminolevulinic acid or derivative thereof - Google Patents
Biostimulant comprising plant immunoactive protein and 5-aminolevulinic acid or derivative thereof Download PDFInfo
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- CN113875778A CN113875778A CN202111148587.7A CN202111148587A CN113875778A CN 113875778 A CN113875778 A CN 113875778A CN 202111148587 A CN202111148587 A CN 202111148587A CN 113875778 A CN113875778 A CN 113875778A
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- aminolevulinic acid
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Microbiology (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a biostimulator containing plant immune active protein and 5-aminolevulinic acid or derivatives thereof, which comprises two effective components of the plant immune active protein and the 5-aminolevulinic acid or derivatives thereof, wherein the weight ratio of the two components is (0.05-15): 1. the plant immune activity protein and the 5-aminolevulinic acid or the derivative thereof in the biological stimulant have mutual synergistic improvement effect, and have obvious effects of increasing the yield of crops, resisting diseases, improving the quality of the crops and the like. The concrete expression is as follows: 1. the two components can synergistically improve the immunity and disease resistance of crops. 2. The two active components have different action mechanisms and can obviously improve the crop yield and the crop quality in a synergistic way.
Description
Technical Field
The invention belongs to the field of biological agriculture, and particularly relates to a biological stimulator composition capable of promoting synergistic growth and yield increase of crops, in particular to a biological stimulator composition containing plant immune active protein and 5-aminolevulinic acid or derivatives thereof.
Background
The plant immune activity protein is a general name of a pathogenic protein exciton extracted from screened fungus or bacterial protein, is a natural protein derived from fungus microorganisms, is used as a magic special extracellular signal outside plant cells based on a cell signal transduction path, can efficiently excite a plant integral immune system and a plant rapid growth system through signal conduction of a plant receptor (mainly a plant leaf cell membrane receptor), enables the plant to rapidly generate stress-resistant protection mechanisms such as disease resistance, drought resistance, freeze resistance and the like, rapidly promotes the plant to generate growth promoting factors, and improves the plant growth quantity.
5-aminolevulinic acid is used as a novel photoactivation product with high environmental compatibility and selectivity, has very wide application in the agricultural field, and can adjust the synthesis of chlorophyll of crops; the stability of chlorophyll and a light capturing system II is improved; the photosynthetic efficiency is improved, and the photosynthesis is promoted; promoting plant tissue differentiation, inhibiting respiration in dark, enlarging stomata, etc. Therefore, the plant growth regulator is not simply a biological metabolism intermediate product, is also involved in the regulation process of plant growth and development, has the physiological activity similar to plant hormone, and can be used as a plant growth regulator or a novel fertilizer in agricultural production.
At present, in the process of planting agricultural crops, due to long-term unreasonable use of fertilizers and pesticides, such as chemical application of elementary substances high nitrogen, high phosphorus and the like to regulate the growth of crops and improve the yield of the crops, and long-term application of chemical phosphorus-containing pesticides, the concentration of single nutrients in soil is too high, so that the phase reaction stock in the soil is too large, harmful substances are generated in the soil and become main factors for hardening the soil in partial areas, the yield of the crops is reduced on the contrary, the quality of agricultural products is reduced, and the natural development rule of the ecological environment of the soil is violated, so that a novel biological fertilizer is created, the fertilizer is applied reasonably, the comprehensive utilization efficiency of the fertilizer is improved, the life of the soil is activated, and the method is an important means for realizing sustainable development of the fertilizer industry.
Disclosure of Invention
The invention aims to solve the technical problem of providing a safe and environment-friendly bio-stimulant composition capable of synergistically promoting plant growth aiming at the defects in the prior art. The inventor finds that the 5-aminolevulinic acid or the derivative thereof and the plant immune active protein have obvious synergistic effect in the aspects of promoting plant growth and improving crop yield.
It is another object of the present invention to provide the use of a biostimulant composition comprising a plant immunoactive protein and 5-aminolevulinic acid or a derivative thereof in the field of agriculture for promoting plant growth and increasing crop yield.
The purpose of the invention can be realized by the following technical scheme:
a biological stimulator composition comprises plant immune active protein and 5-aminolevulinic acid or derivatives thereof as main active ingredients, wherein the weight ratio of the plant immune active protein to the 5-aminolevulinic acid is (0.05-15): 1, preferably (0.1 to 15): 1.
as a preferable technical scheme, the derivative of the 5-aminolevulinic acid is selected from 5-aminolevulinic acid ester derivatives or 5-aminolevulinic acid salt derivatives; wherein, the 5-aminolevulinic acid ester derivative is preferably methyl 5-aminolevulinic acid, ethyl 5-aminolevulinic acid, propyl 5-aminolevulinic acid, n-butyl 5-aminolevulinic acid or n-hexyl 5-aminolevulinic acid; the 5-aminolevulinic acid salt derivative is preferably 5-aminolevulinic acid hydrochloride, 5-aminolevulinic acid phosphate or 5-aminolevulinic acid sulfonate.
As a preferred embodiment, at least one of the above-mentioned plant immunoactive proteins (1) to (3):
(1) the plant immune activity protein is a protein which is secreted or metabolized in gram-negative plant pathogenic bacteria and has growth promoting or immune activity to plants, preferably a hypersensitive protein (Harpin);
(2) a protein, preferably Cryptogein, produced by secretion or metabolism of a plant cryptophyceae and having growth promoting or immunological activity to the plant;
(3) a protein which is screened, separated or purified from plant pathogenic fungi and has growth promoting or immunological activity to plants; an Activator (Activator) is preferred, at least one of an activin of Alternaria tenuis, an activin of Verticillium dahliae, an activin of Botrytis cinerea, an activin of Penicillium, an activin of Alternaria alternata and an activin of Pyricularia oryzae is further preferred, and at least one of an activin of Alternaria tenuis and an activin of Verticillium dahliae is further preferred.
As a preferable technical scheme, the biostimulant composition comprises an effective component and a pesticide auxiliary agent, and the effective component and the pesticide auxiliary agent are prepared into a pesticide allowable dosage form.
In order to facilitate use and improve use effect, the biological stimulator composition can be added with other fertilizers, especially water-soluble fertilizers for co-application, and at least one of humic acid and amino acid can be added compositely. The humic acid is preferably potassium fulvate, and the content of the humic acid accounts for 2-20% (w/w) of the total content of the composition.
In the invention, other amino acids can be added besides 5-aminolevulinic acid, the other amino acids mainly comprise one or a mixture of more of threonine, arginine, aspartic acid, alanine, glutamic acid, histidine, isoleucine, leucine, lysine, proline, methionine, phenylalanine, tryptophan, serine, tyrosine, valine and the like, preferably animal-derived amino acids, and the content of the amino acids accounts for 2-18% (w/w) of the total content of the composition.
In one embodiment of the present invention, to further enhance the effect of the present stimulant composition, one or more known microbial agents may be added. Suitable microorganisms include, but are not limited to, a mixed microbial species selected from one or more of bacillus subtilis, bacillus licheniformis, bacillus thuringiensis, bacillus amyloliquefaciens, bacillus laterosporus, trichoderma harzianum, rhizobium, or paecilomyces lilacinus. In the invention, the total amount of the effective viable bacteria is more than 0.2 hundred million/g, and in order to further improve the use effect, the total amount of the effective viable bacteria of the microorganism bacteria is preferably more than 2 hundred million/g, and the total amount of the effective viable bacteria of the microorganism bacteria is further preferably more than 10 hundred million/g.
Furthermore, the composite biological preparation containing the 5-aminolevulinic acid or the derivative thereof and the plant immune active protein also contains organic matters. The organic content is greater than 8% (w/w), preferably greater than 20%. Suitable organic materials include, but are not limited to, one or a mixture of more than one of cellulose, hemicellulose, proteins, lipid materials, asphaltenes, resins and gums, tannins, steroids, vitamins, terpenes, and humus.
The dosage form suitable for the present invention is selected from a solid dosage form or a liquid dosage form. The solid dosage form is preferably a powdered or granular solid dosage form. The liquid dosage form is preferably a soluble solution dosage form or a suspension dosage form.
The adjuvants involved in the above-described active ingredient formulations include, but are not limited to, binders, wetting agents, dispersants, emulsifiers, penetrants, preservatives, antifreeze agents, fillers, carriers, colorants, antifoams, evaporation inhibitors, and pH and viscosity modifiers, each of which is conventional.
Suitable surfactants (penetrants) include, but are not limited to, one or more of the known emulsifiers such as fatty alcohol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol polyoxyethylene polyoxypropylene ether, phenethylphenol polyoxyethylene polyoxypropylene ether, polyoxyethylene sorbitan fatty acid ester, and alkylphenol polyoxyethylene phosphate ester. The thickener is one or more of xanthan gum, sodium carboxymethyl (ethyl) cellulose, methylcellulose, polyvinyl alcohol and polyvinylpyrrolidone. The antifreezing agent is one or more of ethylene glycol, propylene glycol, glycerol and polyethylene glycol.
Suitable dispersants include, but are not limited to, aromatic sulfonates, acrylic acid-acrylic acid ester copolymers, formaldehyde condensate sulfonates, maleic anhydride copolymer sodium salts, polymeric carboxylic acid sodium salts, styrene alkyl polyoxyethylene ether phosphate esters, sodium naphthalene sulfonate formaldehyde condensates, tristyrylphenol polyoxyethylene ethers and derivative esters, fatty alcohol polyoxyethylene ethers, alkylphenol polyoxyethylene ethers, lignosulfonates, alkylphenol polyoxyethylene formaldehyde condensate sulfates, alkylphenol polyoxyethylene formaldehyde condensate sulfate phosphates, styrene phenol formaldehyde resin polyoxyethylene ether phosphate esters, styrene phenol polyoxyethylene polyoxypropylene ethers, alkylnaphthalene formaldehyde condensate sulfonates, polymeric hydrophobic branched copolymers, polymeric carboxylates, and combinations thereof.
Suitable fillers include, but are not limited to, one of modified water soluble starch, cellulose ether and its derivatives, magnesium aluminum silicate, kaolin, diatomaceous earth, attapulgite, glucose, precipitated calcium carbonate, white carbon, inorganic salts.
Suitable wetting agents include, but are not limited to, sodium lauryl sulfate, nonylphenol polyoxyethylene, alkylphenol polyoxyethylene formaldehyde condensate sulfate phosphate, styrene phenol formaldehyde resin polyoxyethylene ether phosphate, styrene phenol polyoxyethylene polyoxypropylene ether, alkyl naphthalene formaldehyde condensate sulfonate, alkyl succinate sulfonate, Tween, or combinations of two or more thereof.
Suitable adjuvants include, but are not limited to, one or a combination of several of binders, disintegrants, pH adjusting agents, antimicrobial preservatives, antifoaming agents, anti-freezing agents, and thickeners; wherein: the binder comprises but is not limited to polyvinyl alcohol, gelatin, Arabic gum, xanthan gum, sodium polyacrylate, starch, cellulose and derivatives thereof, preferably one of cellulose and cellulose derivatives thereof; the disintegrating agent is selected from one of magnesium aluminum silicate, polyethylene glycol, modified starch, inorganic salt, white carbon black and silica gel; the pH acid-base regulator comprises but is not limited to one of glacial acetic acid, hydrochloric acid, ammonia water, ethanolamine and triethanolamine; the sterilizing preservative comprises but is not limited to one of potassium sorbate, sodium benzoate, formaldehyde and isothiazolinone; the defoaming agent comprises one of natural grease, polyether defoaming agent, higher alcohol and organic silicon defoaming agent. The antifreeze agent includes, but is not limited to, propylene glycol or ethylene glycol; the thickener includes, but is not limited to, xanthan gum, carrageenan, pectin, agar, alginate jelly, or sodium carboxymethylcellulose.
Further, the solid dosage form may be processed into a granular, flaky, particulate or powdery form, preferably granular or powdery form, depending on the subject of administration.
In one of the processing technical schemes of the invention, the method comprises the following steps: 1) according to the ratio of plant immune active protein and 5-aminolevulinic acid or derivatives thereof (0.05-15): 1, uniformly mixing; 2) pulverizing the uniformly stirred mixture to 20-70 mesh, and 3) quantitatively packaging to obtain a powdery preparation.
In the processing step of the step 2, after the crushing is finished, the granules can be granulated, dried and cooled to prepare granular preparations.
In the above solid preparation processing scheme, one or more than one purchased or cultured microbial agents can be uniformly mixed, and the microbial agents and the components of the invention are uniformly mixed and stirred, so that the effective viable count of each gram of fertilizer is not less than 0.2 hundred million (preferably not less than 2 hundred million per gram of fertilizer), and the powdery or granular composite microbial preparation is prepared.
In order to further improve the effect, in the processing technical scheme, organic matters with certain weight can be added and crushed together. The content of organic matters is higher than 8%, and cellulose, hemicellulose and protein are preferable as the organic matters.
In the processing scheme of the solid preparation, proper humic acid, amino acid or trace elements can be added according to the actual soil structure and the growth requirements of different crops. Mixing the required elements with the components of the invention according to the weight ratio, and crushing. Or mixing the required elements with the components of the invention containing microbial agents or organic matters according to the weight ratio and crushing. Finally preparing into solid preparations such as powder or granules.
Use of a biostimulant composition as described above for promoting crop growth and increasing crop yield.
The plant immune active protein and the 5-aminolevulinic acid or the derivative thereof in the composition have mutual synergistic improvement effect, and the specific expression is as follows: 1. the two components can synergistically improve the immunity and disease resistance of crops. 2. The two active components have different action mechanisms and can obviously improve the crop yield and the crop quality in a synergistic way.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the embodiments described herein are merely illustrative of the present invention and are not intended to limit the invention, and any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the scope of the present invention.
The percentages in all formulations in the following examples are by weight. The processing technology of various preparations of the composition is the prior art, and can be changed according to different conditions.
First, preparation examples of dosage forms
Mixing the plant immune active protein and 5-aminolevulinic acid or salt thereof with the auxiliary agent and the filler uniformly according to the proportion of the formula, and carrying out air flow crushing to obtain a powder formulation product.
Example 1 3.2% Crypthecodinin 5-aminolevulinic acid hydrochloride powder
3% of cryptogeon, 0.2% of 5-aminolevulinic acid hydrochloride, 5% of fatty amide N-methyl taurine sodium salt, 0.8% of organic silicon, 3% of calcium alkyl benzene sulfonate, 8% of animal-derived amino acid and the balance of diatomite to 100%.
Example 2 powder of 4.2% Crypthecodinin 5-aminolevulinic acid methyl ester
4 percent of cryptogeon, 0.2 percent of methyl 5-aminolevulinic acid, 2 percent of Chinese honeylocust fruit powder, 0.9 percent of organic silicon, 10.5 percent of potassium fulvate, 3 percent of magnesium aluminum silicate and white carbon black which are complemented to 100 percent.
Example 3 hypersensitivity protein 5-aminolevulinic acid phosphate powder 10.5%
0.5 percent of hypersensitive protein, 10 percent of 5-aminolevulinic acid phosphate, 10 percent of cellulose, 26.25 percent of glutamic acid, 2 percent of sodium dodecyl benzene sulfonate and white carbon black which are complemented to 100 percent.
Example 4 hypersensitivity protein 5-aminolevulinic acid sulfonate powder 3%
1.5 percent of hypersensitive protein, 1.5 percent of 5-aminolevulinic acid sulfonate, 4 percent of alkylphenol polyoxyethylene phosphate, 7.5 percent of valine, 3 percent of alkyl aryl sulfonate, 3 percent of bentonite and white carbon black which are complemented to 100 percent.
Example 5 wettable powder of 3.3% Crypthecodinium 5-aminolevulinic acid n-butyl ester
0.3 percent of cryptogeon, 3 percent of 5-aminolevulinic acid n-butyl ester, 3 percent of lauryl sodium sulfate, 8.25 percent of humic acid, 7.5 percent of polymeric sodium carboxylate, 3.6 percent of alkyl succinate sulfonate and the balance of white carbon black to 100 percent.
Example 6 wettable powder of 2.1% Crypthecodinium 5-aminolevulinic acid Ethyl ester
0.1% of cryptogeon, 2% of 5-aminolevulinic acid ethyl ester, 0.8% of saponin powder, 5.25% of bacillus amyloliquefaciens (20 hundred million spores per gram), 20% of plant ash, 7.2% of lignosulfonate, 0.5% of organic silicon and the balance of diatomite to 100%.
(II) granules
Uniformly mixing the plant immune active protein, 5-aminolevulinic acid or salt thereof, an auxiliary agent and a filler according to the proportion of the formula, performing jet milling, drying by a granulation machine after the milling is finished, and automatically cooling to prepare the granular preparation.
Example 7 granules of 4% Alternaria tenuissima activator protein 5-aminoacetyl hydrochloride
2% of alternaria tenuissima activator protein, 2% of 5-aminolevulinic propionate hydrochloride, 6.2% of formaldehyde condensate sulfonate, 5.4% of alkylphenol polyoxyethylene formaldehyde condensate sulfate, 10% of humic acid and the balance of attapulgite to 100%.
Example 8 Ethyl 5-aminolevulinate 3.2% Alternaria tenuissima activator granules
3% of alternaria tenuissima activator protein, 0.2% of 5-aminolevulinic acid ethyl ester, 8% of leucine, 5% of polymeric potassium carboxylate, 47% of alkyl succinate sulfonate and the balance of diatomite to 100%.
Example 9 Alternaria tenuissima activator protein 5-aminolevulinic acid sulfonate granules 10.5%
0.5 percent of Alternaria tenuissima activator protein, 10 percent of 5-aminolevulinic acid sulfonate, 26.3 percent of bacillus subtilis (20 hundred million spores/gram), 5.9 percent of sodium dodecyl sulfate and white carbon black which are complemented to 100 percent.
Example 10 hypersensitivity protein 5-aminolevulinic acid phosphate granules 2.1%
2% of hypersensitive protein, 0.1% of 5-aminolevulinic acid phosphate, 5% of lignosulfonate, 5.5% of phenylalanine, 3.5% of magnesium aluminum silicate, 10% of plant ash, 5.4% of bentonite and the balance of white carbon black to 100%.
Example 11 granules of 2.2% Verticillium dahliae activator protein 5-aminolevulinic acid n-butyl ester
0.2 percent of verticillium dahliae activator protein, 2 percent of 5-aminolevulinic acid n-butyl ester, 5 percent of polymeric carboxylic acid sodium salt, 4 percent of alkyl succinate sulfonate, 5.5 percent of animal source amino acid, 2 percent of ammonium sulfate and the balance of attapulgite to 100 percent.
Example 12 hypersensitivity protein 5-aminolevulinic acid hydrochloride granules of 3.2%
3% of hypersensitive protein, 0.2% of 5-aminolevulinic acid hydrochloride, 3% of sodium benzenesulfonate, 8% of potassium fulvate, 5% of alkylphenol polyoxyethylene ether phosphate, 12% of polyethylene glycol, 2% of bentonite and the balance of diatomite to 100%.
Example 13 hypersensitivity protein 5-aminolevulinic acid phosphate granules (1: 10)
0.5 percent of hypersensitive protein, 5 percent of 5-aminolevulinic acid phosphate, 2 percent of alkyl naphthalene formaldehyde condensate sulfonate, 3 percent of magnesium aluminum silicate, 7 percent of bentonite, 13.75 percent of aspartic acid and the balance of attapulgite to 100 percent.
Example 14.13% Alternaria tenuissima activator protein 5-aminolevulinic acid sulfonate granules (1: 25)
0.5 percent of Alternaria tenuissima activator protein, 12.5 percent of 5-aminolevulinic acid sulfonate, 3 percent of bentonite, 1 percent of polyethylene glycol, 5 percent of calcium dodecyl sulfate, 31.25 percent of bacillus cereus (10 hundred million spores/gram) and white carbon black which are complemented to 100 percent.
Example 15 wettable powder of 3% Crypthecodinium 5-aminolevulinic acid hydrochloride
1% of cryptogeon, 2% of 5-aminolevulinic acid hydrochloride, 3% of xanthan gum, 3% of alkylphenol polyoxyethylene formaldehyde condensate sulfate, 7.5% of potassium fulvate, 2% of sodium bicarbonate and the balance of kaolin to 100%.
Second, efficacy test
The test is carried out in a demonstration base of a modern agriculture science and technology park in Yangtze city Dafeng district in Jiangsu province, 11-month No. 18 sowing is carried out in 2019, and 5-month harvesting is carried out in 2020. The main nutrients of the tested soil were as follows: 58.31g/kg of organic matter, 4.19g/kg of total nitrogen, 5.34g/kg of total potassium, 629mg/kg of available phosphorus, 573mg/kg of quick-acting potassium and 8.2 of pH. For supplying toThe tested oat variety is sweet swallow No. two. Before sowing, the applied fertilizer is uniformly applied to the cell for 1 time, and then is deeply turned and harrowed. The area of the cell is 2m by 5m, 7 rows are planted, and the seeding rate is 120kg/hm2The row spacing is 25cm, the drill sowing is carried out, and the sowing depth is 3-4 cm. Performing field management and field production, and performing normal impurity removal and other conventional management measures; the test field has irrigation conditions, irrigation is carried out once after seeding and in the jointing stage, each cell is irrigated respectively, and irrigation is stopped when 1cm of water is accumulated on the surface layer of the cell soil.
The spraying is carried out once on the leaf surfaces of No. 3/month No. 13 and No. 3/month No. 21 in 2020. The test was carried out using 16 treatments, namely CK (control, same amount of clear water), example 1, example 2, example 3, example 4, example 5, example 6, example 7, example 8, example 9, example 10, example 11, example 12, example 13, example 14 and example 15. Each treatment was set to 3 replicates. The control agents used were consistent with the processing methods and formulations performed.
And (4) sampling in the field at 11 days 4 and 4 in 2020, and determining the occurrence of leaf spot of oat leaves. The samples were collected at 28 days 5 and 28 in 2020, and 20 plants were randomly sampled from each cell. The influence of each example on the growth of crops is determined, and the conditions such as the yield of the oat seeds, the content of beta-glucan and the like are comprehensively evaluated (the content of beta-glucan is determined by Congo red spectrophotometry). In the test, the indexes such as yield, beta-glucan and leaf spot disease index are all average values of each treatment.
Grading standard:
level 0: no disease spots;
level 1: the number of the leaf spots is less than 5, and the length is less than 1 cm;
and 3, level: 6-10 leaf spots, wherein the length of part of the spots is more than 1 cm;
and 5, stage: 11-25 leaf spots, wherein part of the spots are connected into pieces, and the area of the spots accounts for 10-25% of the area of the leaves;
and 7, stage: the number of the scab of the leaf is more than 26, the scab is connected into pieces, and the area of the scab accounts for 26-50% of the area of the leaf;
and 9, stage: the disease spots are connected into tablets, and the area of the disease spots accounts for more than 50 percent of the area of the leaves or the whole leaves die.
The yield per mu is increased to 100 percent (the processed yield per mu-the yield per mu of the clear water contrast)/the yield per mu of the clear water contrast
The increase in β -glucan content (treated β -glucan content- β -glucan content of clear water control)/β -glucan content of clear water control 100%
The prevention rate of leaf spot is (clear water control leaf spot index-incidence index of treated leaf spot)/clear water control powdery mildew incidence index is 100%
TABLE 1 summary of the combined oat growth regulation test using plant immunoactive protein and 5-aminolevulinic acid
The experiment shows that the plant immune active protein and the 5-aminolevulinic acid have good synergistic promotion effect on oat growth, and the main effect is that the plant immune active protein and the 5-aminolevulinic acid are combined in the ratio of (0.05-15): the prevention effect on the leaf spot of oat is remarkable between 1, wherein the synergistic effect is most remarkable in the following cases of example 7 (4% verticillium dahliae activator protein.5-aminolevulinic acid hydrochloride granules, the ratio is 2:2), example 4 (3% hypersensitive protein.5-aminolevulinic acid sulfonate powder, the ratio is 1.5: 1.5) and example 15 (3% cryptogeon.5-aminolevulinic acid n-butyl ester wettable powder, the ratio is 1: 2), and the ratio of the plant immune activity protein to the 5-aminolevulinic acid is (0.05-15): the control effect is not obvious except for 1 proportion, and the control effect is only additive effect in three examples such as example 14 (13% Alternaria tenuissima activator protein 5-aminolevulinic acid sulfonate granules, proportion 1: 25), example 10 (2.1% hypersensitivity protein 5-aminolevulinic acid phosphate granules, proportion 20: 1) and example 2 (3.8% cryptobiosin 5-aminolevulinic acid methyl ester powder, proportion 20: 1). Through detection, the ratio of the plant immune active protein to the 5-aminolevulinic acid (0.05-15) is as follows: 1, the two compositions have obvious effect on improving the yield of the oat seeds, wherein the yield increasing effect is most obvious by using example 4 (3% hypersensitive protein 5-aminolevulinic acid sulfonate powder, the ratio is 1.5: 1.5), example 7 (4% verticillium dahliae actiprotein 5-aminolevulinic acid hydrochloride granules, the ratio is 2:2) and example 15 (3% cryptomerin 5-aminolevulinic acid n-butyl ester wettable powder, the ratio is 1: 2), and the yield increasing rate respectively reaches 27.96%, 26.93% and 26.48%. Through the determination of a key oat quality index beta-glucan, the two components are (0.05-15): 1, the two can obviously improve the content of the beta-glucan in a synergistic way. Therefore, the comprehensive tests on the disease resistance, unit yield, quality and the like of the oat show that the plant immune activity protein and the 5-aminolevulinic acid or the derivative thereof are as follows (0.05-15): 1 has obvious synergistic and promoting effect on crops.
Claims (9)
1. A biostimulant composition characterized by: the biological stimulator composition takes plant immune active protein and 5-aminolevulinic acid or derivatives thereof as main effective components, and the weight ratio of the plant immune active protein to the 5-aminolevulinic acid or derivatives thereof is (0.05-15): 1; preferably, the weight ratio of the plant immune active protein to the 5-aminolevulinic acid or the derivative thereof is (0.1-15): 1.
2. the biostimulant composition of claim 1, wherein: the derivative of the 5-aminolevulinic acid is selected from 5-aminolevulinic acid ester derivatives or 5-aminolevulinic acid salt derivatives.
3. The biostimulant of claim 2, wherein: the 5-aminolevulinic acid ester derivative is preferably methyl 5-aminolevulinic acid, ethyl 5-aminolevulinic acid, propyl 5-aminolevulinic acid, n-butyl 5-aminolevulinic acid or n-hexyl 5-aminolevulinic acid; the 5-aminolevulinic acid salt derivative is preferably 5-aminolevulinic acid hydrochloride, 5-aminolevulinic acid phosphate or 5-aminolevulinic acid sulfonate.
4. The biostimulant composition of claim 1, wherein: the plant immune active protein is at least one of the following (1) to (3):
(1) a protein secreted or metabolized by gram-negative plant pathogenic bacteria that has growth promoting or immunological activity in plants;
(2) a protein which is screened, separated or purified from plant pathogenic fungi and has growth promoting or immunological activity to plants;
(3) a protein produced by the secretion or metabolism of a plant cryptophyte mould and having growth-promoting or immunological activity to the plant.
5. The biostimulant composition of claim 1 or 4, wherein: the plant immune active protein is a hypersensitive protein, a cryptogeon protein or an activator protein.
6. The biostimulant composition of claim 5, wherein: the activator protein is selected from the group consisting of Alternaria tenuissima activator protein, Verticillium dahliae activator protein, Botrytis cinerea activator protein, Penicillium activator protein, Alternaria alternata activator protein and Magnaporthe grisea activator protein; preferably Alternaria gracilis activator protein or Verticillium dahliae activator protein.
7. A biostimulant composition according to any one of claims 1 to 6, wherein: the biological stimulant composition comprises an effective component and a pesticide auxiliary agent, and the effective component and the pesticide auxiliary agent are prepared into pesticide allowable dosage forms.
8. The biostimulant composition of claim 7, wherein: the biostimulant composition further comprises a fertilizer component; the fertilizer component comprises at least one component of water-soluble fertilizer, humic acid, amino acid, organic matters or microbial agent; the content of humic acid accounts for 2-20% of the total amount of the biological stimulant composition; the content of the amino acid accounts for 2-18% of the total amount of the biostimulant composition; said organic matter is present in said biostimulant composition in an amount greater than 8%; the amount of the microorganism in the biostimulant composition is more than or equal to 0.2 hundred million/gram.
9. Use of a biostimulant composition according to claims 1 to 8 for promoting crop growth and increasing crop yield.
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Application publication date: 20220104 |