Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are a part of the embodiments of the present invention, but not all of the embodiments. Thus, the following detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Some embodiments of the invention provide a multiple-effect nutritional composition for cotton seedling emergence in saline-alkali soil, which comprises the following raw materials in percentage by weight: 0.05-0.3% of saline-alkali tolerant trichoderma harzianum, 0.5-1% of bacillus thuringiensis, 0.05-0.3% of streptomyces rochei, 25-38% of biochemical fulvic acid potassium, 40.0-60.0% of compound amino acid and auxiliary materials.
The saline-alkali tolerant trichoderma harzianum strain number is 29HB16, and the registration number of the common microorganism center of China Committee for culture Collection of microorganisms is CGMCC No. 7975. The trichoderma harzianum 29HB16 can tolerate salt stress caused by salt concentration below 80g/L and alkali stress below pH8.0. The trichoderma harzianum 29HB16 of the invention is introduced into saline-alkali soil, can effectively prevent and treat fungal diseases through biological control, can effectively improve the environment and maintain the balance of an agricultural ecological system.
Preferably, the weight percentage of the saline-alkali tolerant trichoderma harzianum is 0.05-0.3%. For example, in some embodiments of the invention, the saline-alkali tolerant trichoderma harzianum is 0.05%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3% by weight.
For example, in some embodiments of the invention, the weight percentage of bacillus thuringiensis is 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%. For example, in some embodiments of the invention, the weight percentage of Streptomyces rochei is 0.05%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%. For example, in some embodiments of the invention, the biochemical fulvic acid potassium weight percentage is 25%, 28%, 30%, 32%, 35%, 38%. For example, in some embodiments of the invention, the composite amino acid weight percentage is 40%, 45%, 50%, 55%, 60%.
Bacillus thuringiensis, a type of crystal-producing Bacillus that includes many varieties. The bacterium can produce two major toxins, namely endotoxin (parasporal crystals) and exotoxin, so that pests stop eating the bacterium, and finally the pests die due to starvation and poisoning. Therefore, the bacillus can be used as a microbial source low-toxicity insecticide for controlling various pests of orthoptera, coleoptera, diptera and hymenoptera, particularly lepidoptera.
The compound amino acid comprises 18 amino acids such as glycine, alanine, leucine, isoleucine, valine, cystine, cysteine, methionine, threonine, serine, phenylalanine, tyrosine, tryptophan, proline, hydroxyproline, glutamic acid, aspartic acid and the like. Meanwhile, the compound amino acid has certain bonding capacity, so that the dissipation of other combinations is effectively protected, and the sizing effect of the granular materials is enhanced. In the embodiment of the invention, the compound amino acid is used as a nutrient of saline-alkali-resistant trichoderma harzianum, bacillus thuringiensis and streptomyces rochei to promote the growth and the propagation of microorganisms, and the saline-alkali-resistant trichoderma harzianum, bacillus thuringiensis and streptomyces rochei are wrapped by the compound amino acid, so that the damage of the three bacteria by other components of the invention is reduced, and the reduction of the action of the bacteria is avoided. In addition, the composite amino acid is slightly acidic, so that the slightly alkaline property of the saline-alkali soil can be improved.
The biochemical potassium fulvate contains high-content fulvic acid, and is also rich in almost all amino acids, nitrogen, phosphorus, potassium, various enzymes, saccharide (oligosaccharide, fructose and the like) proteins, nucleic acid, humic acid, VC, VE, a large amount of B vitamins and other nutritional ingredients required in the plant growth process, and is a green humic acid fertilizer. The biochemical potassium fulvate has unknown growth promoting factors with high biological activity, and can promote the seedling emergence and the rapid growth of the saline-alkali soil cotton. The biochemical fulvic acid potassium has strong complexing ability, and improves the absorption and operation of trace elements of plants. The absorption of trace element fertilizers, such as zinc fertilizers, boron fertilizers and trace elements in soil in the multi-effect nutrient composition for cotton emergence in saline-alkali lands provided by the embodiment of the invention is promoted.
The biochemical potassium fulvate serving as the multiple-effect nutrient composition for the cotton seedling emergence in the saline-alkali land provided by the embodiment of the invention has the main functions that: one is to enhance the activity of oxidase and other metabolic activities in the plant. And secondly, the growth of the plant root system is promoted, the activity of the root system is improved, the absorption of the plant to water and nutrient elements is facilitated, the chlorophyll content is improved, the photosynthesis is enhanced, and the stress resistance of the plant is improved. And thirdly, the pesticide is compounded with the pesticide for use, so that the pesticide can inhibit pathogenic bacteria spore germination and pathogenic bacteria colony expansion, and is beneficial to slow release and synergy of the pesticide. Fourthly, the fertilizer has the obvious functions of cold resistance and drought resistance.
The drought-resistant cold-resistant mechanism of the biochemical potassium fulvate is as follows: firstly, the activity of the enzyme in the plant body is stimulated, and the cell in the plant body is catalyzed to accelerate the absorption of water and nutrient through the regulation of the enzyme, so that the water potential of the leaves is reduced, and the metabolic activities such as osmotic pressure and the like are enhanced, so that the plant is stimulated to adapt to the drought and cold living environment physiologically. And secondly, the accumulation of K in guard cells is inhibited, the water retention capacity of the plant can be improved by self when the plant encounters drought and cold, the air holes of the leaves are closed or the opening degree is reduced, and the water transpiration is reduced.
The Streptomyces rochei enables seed inoculation to have antagonistic action, and the Streptomyces rochei metabolizes to generate enzyme and antibiotic for preventing pathogenic bacteria from invading, so that a protective layer is formed around the plant root to inhibit the propagation of the pathogenic bacteria infected firstly.
In some embodiments of the invention, the auxiliary materials comprise the following components in percentage by weight of the multi-effect nutritional composition for cotton seedling emergence in saline-alkali soil: 5.0 to 8.0 percent of phosphorus-nitrogen fertilizer, 1.5 to 3.0 percent of zinc fertilizer, 1.2 to 2.3 percent of boron fertilizer, 0.05 to 0.08 percent of 4-aminobutyric acid, 0.08 to 0.15 percent of chitosan and the balance of amino acid. For example, in some embodiments of the invention, the phosphorus-nitrogen fertilizer is 5.0%, 5.5%, 6.0%, 6.5%, 7.0%, 7.5%, 8.0% by weight. For example, in some embodiments of the invention, the zinc fertilizer is 1.5%, 2.0%, 2.5%, 3.0% by weight. For example, in some embodiments of the present invention, the percentage by weight of the boric fertilizer is 1.2%, 1.4%, 1.6%, 1.8%, 2.1%. For example, in some embodiments of the invention, the 4-aminobutyric acid weight percentage is 0.05%, 0.06%, 0.07%, 0.08%. For example, in some embodiments of the invention, the chitosan weight percentage is 0.08%, 0.10%, 0.12%, 0.14%, 0.15%.
4-aminobutyric acid is a non-protein amino acid, can participate in various physiological metabolic activities, is an inducer for plants, and is a neuro-inhibitor for pests. The cotton root can induce the improvement of the activity of the resistant enzyme after being absorbed, and enhance the synthesis of lignin, thereby preventing the invasion of pathogenic bacteria. The composition can be used for synergistically inhibiting the activity of underground pests, inducing the disease resistance of cotton and promoting the growth of cotton roots.
Chitosan acts as a thickener and is readily soluble in weakly acidic solvents, and it is particularly noted that the dissolved solution contains amino groups (NH2+), which inhibit bacteria by binding negative electrons.
In some embodiments of the invention, the phosphorus-nitrogen fertilizer is ammonium metaphosphate, and ammonium metaphosphate is neutral, which is a preferred embodiment. In other embodiments the phosphorus nitrogen fertilizer may also be monoammonium phosphate.
In some embodiments of the invention, the zinc fertilizer is amino acid zinc, and the zinc fertilizer is selected from amino acid zinc, and provides nitrogen fertilizer and amino acid besides zinc element. The boric fertilizer is boric acid, and can improve the alkalinity of saline-alkali soil because the boric acid is acidic in addition to providing boron elements.
In some embodiments of the invention, the multi-effect nutrient composition for cotton emergence in saline-alkali land is in the form of wettable powder, water aqua or water dispersible granule. The saline-alkali land is mostly gray desert soil developed by Yidao, water shortage and desertification are typical characteristics, the multiple-effect cotton seedling-emergence nutrient composition in the saline-alkali land provided by some embodiments of the invention is a full water-soluble composition, the dosage is small, the application is convenient, the effect is quick, and various problems of cotton seedling emergence can be solved in time.
Some embodiments of the invention provide a preparation method of a multiple-effect cotton seedling emergence nutrient composition for saline-alkali lands, which is characterized in that raw materials of the multiple-effect cotton seedling emergence nutrient composition for the saline-alkali lands are mixed according to weight percentage.
In some embodiments of the invention, the mixing comprises: mixing saline-alkali tolerant trichoderma harzianum, bacillus thuringiensis, streptomyces rochei and compound amino acid according to weight percentage to obtain a first material; the first mix may be purchased as a product mixed and packaged according to the above formulation, or may be mixed rapidly by itself.
Mixing biochemical fulvic acid potassium, a phosphorus-nitrogen fertilizer, a zinc fertilizer, a boron fertilizer, 4-aminobutyric acid and chitosan according to weight percentage to obtain a second material; the chitosan is used as a thickening agent to bond biochemical fulvic acid potassium, a phosphorus-nitrogen fertilizer, a zinc fertilizer, a boron fertilizer and 4-aminobutyric acid as second materials, so that the influence on thalli caused by the fact that the second materials are dispersed and contacted with the three thalli is avoided. It is likewise possible to purchase a product packaged in a mixture of the formulation proportions or to carry out a rapid mixing on its own.
And mixing the first material and the second material to obtain the multi-effect cotton seedling emergence nutrient composition for the saline-alkali soil.
Some embodiments of the invention provide an application method of a multi-effect nutritional composition for cotton seedling emergence in saline-alkali soil, which comprises the steps of dissolving the nutritional composition for cotton in the saline-alkali soil in water for application at an application rate of 1.0-2.0 kg/mu after cotton planting; or applying the cotton nutrient composition in the saline-alkali land at the application rate of 3.0-4.0 kg/mu before sowing the cotton.
Next, in combination with the above, the saline alkali cotton emergence multi-effect nutrient composition of the present invention is exemplified by the following specific examples:
example 1
0.05 percent of saline-alkali tolerant trichoderma harzianum, 0.5 percent of bacillus thuringiensis, 0.05 percent of streptomyces rochei and 40.0 percent of compound amino acid are metered into a stirrer according to the components and evenly mixed to obtain a first material.
The biochemical fulvic acid potassium 38%, ammonium metaphosphate 5.0%, amino acid zinc 1.5%, boric acid 1.2%, 4-aminobutyric acid 0.05%, chitosan 0.08% and the rest amino acid are uniformly mixed according to the components to obtain a second material.
And adding the first material into the second material, fully stirring, and uniformly mixing to obtain the multi-effect cotton seedling emergence nutrient composition for the saline-alkali soil.
When seedling emergence water is dripped after cotton seeds are sown, 1.0 kilogram of the composition is applied to every 667 square meter, and the composition is simultaneously applied in a dripping mode when the seedling emergence water is dripped.
Example 2
0.08 percent of saline-alkali tolerant trichoderma harzianum, 0.6 percent of bacillus thuringiensis, 0.10 percent of streptomyces rochei and 45.0 percent of compound amino acid are metered into a stirrer according to the components and are uniformly mixed to obtain a first material.
The biochemical fulvic acid potassium 35%, ammonium metaphosphate 5.50%, amino acid zinc 2.0%, boric acid 1.5%, 4-aminobutyric acid 0.06%, chitosan 0.09% and the rest amino acid are uniformly mixed according to the components to obtain a second material.
And adding the first material into the second material, fully stirring, and uniformly mixing to obtain the multi-effect cotton seedling emergence nutrient composition for the saline-alkali soil.
The composition is used for 3.0 kilograms per 667 square meter of land, is evenly scattered on the ground surface before the cotton is sowed, and is sowed with the cotton after the application.
Example 3
0.10 percent of saline-alkali tolerant trichoderma harzianum, 0.7 percent of bacillus thuringiensis, 0.15 percent of streptomyces rochei and 50.0 percent of compound amino acid are metered into a stirrer according to the components and are uniformly mixed to obtain a first material.
30 percent of biochemical fulvic acid potassium, 6.0 percent of ammonium metaphosphate, 2.5 percent of amino acid zinc, 1.8 percent of boric acid, 0.07 percent of 4-aminobutyric acid, 0.10 percent of chitosan and the rest amino acid are evenly mixed according to the components to obtain a second material.
And adding the first material into the second material, fully stirring, and uniformly mixing to obtain the multi-effect cotton seedling emergence nutrient composition for the saline-alkali soil.
When the water for seedling emergence is dripped after cotton seeds are sown, 1.5 kilograms of the composition is used for every 667 square meters, and the composition is dripped when the water for seedling emergence is dripped.
Example 4
Salt and alkali resistant trichoderma harzianum 0.20%, bacillus thuringiensis 0.9%, streptomyces rochei 0.20% and compound amino acid 55.0% are metered into a stirrer according to the components and evenly mixed to obtain a first material.
28 percent of biochemical fulvic acid potassium, 6.5 percent of ammonium metaphosphate, 2.8 percent of amino acid zinc, 2.0 percent of boric acid, 0.08 percent of 4-aminobutyric acid, 0.12 percent of chitosan and the rest amino acid are evenly mixed according to the components to obtain a second material.
And adding the first material into the second material, fully stirring, and uniformly mixing to obtain the multi-effect cotton seedling emergence nutrient composition for the saline-alkali soil.
The composition is used for every 667 square meters by 3.5 kg, is uniformly scattered on the ground surface before cotton sowing, and is sown after the cotton sowing.
Example 5
Salt and alkali resistant trichoderma harzianum 0.30%, bacillus thuringiensis 1.0%, streptomyces rochei 0.25% and compound amino acid 60.0% are metered into a stirrer according to the components and evenly mixed to obtain a first material.
The biochemical fulvic acid potassium 25%, ammonium metaphosphate 7.0%, amino acid zinc 3.0%, boric acid 2.3%, 4-aminobutyric acid 0.08%, chitosan 0.15% and the rest amino acid are mixed evenly according to the components to obtain a 'second material'.
And adding the first material into the second material, fully stirring, and uniformly mixing to obtain the multi-effect cotton seedling emergence nutrient composition for the saline-alkali soil.
When the water for seedling emergence is dripped after cotton seeds are sown, 1.2 kilograms of the composition is used for every 667 square meters, and the composition is dripped when the water for seedling emergence is dripped.
Example 6
0.25% of saline-alkali tolerant trichoderma harzianum, 0.8% of bacillus thuringiensis, 0.30% of streptomyces rochei and 50.0% of compound amino acid are metered into a stirrer according to the components and are uniformly mixed to obtain a first material.
Uniformly mixing 30% of biochemical fulvic acid potassium, 8.0% of metaphosphoric acid, 2.0% of amino acid zinc, 1.8% of boric acid, 0.08% of 4-aminobutyric acid, 0.15% of chitosan and the rest amino acid according to the components to obtain a second material.
And adding the first material into the second material, fully stirring, and uniformly mixing to obtain the multi-effect cotton seedling emergence nutrient composition for the saline-alkali soil.
The composition is used for every 667 square meters by 3.2 kilograms, and is uniformly scattered on the ground surface before the cotton is sowed, and the cotton is sowed after the cotton is sowed.
Test examples
Selecting saline-alkali soil with equivalent planting conditions in Xinjiang area, and planting Xinjiang Aksu cotton. The test group adopts the multi-effect nutrient composition for cotton seedling emergence in the saline-alkali land provided by the embodiment 3 to carry out drip irrigation, and the control group does not carry out drip irrigation by using the composition provided by the embodiment of the invention, but adopts a composition purchased in the market as the control group.
The cotton seedlings 10 days and 15 days after sowing were compared in quality, and the results are shown in table 1.
TABLE 1 comparison of growth quality of cotton seedlings
According to the table 1, the multi-effect nutritional composition for cotton seedling emergence in the saline-alkali soil is used for fertilizing cotton after seeding, inorganic and organic nutrients for cotton seedling growth are supplied in time in a balanced manner, robust growth of the cotton is promoted, the root system of the cotton is developed, early and rapid growth of the cotton is facilitated, the seedling emergence rate of a test group is 14.3% higher than that of a control group after ten days of seeding, the seedling emergence rate of the test group is 7.7% higher than that of the control group when the cotton is seeded for 15 days, the seedling height of the test group is 1.8% higher than that of the control group, in terms of seedling root number, the test group has 5.6 more than that of the control group on average, the weight of the test group is 7.2g higher than that of the control group in each hundred cotton seedlings, and the vertical and slow rot disease occurrence condition of the test group is 4.4% less than that of the vertical and slow rot disease occurrence condition of the control group.
FIG. 1 is a graph showing the growth of the test group and the control group in the test example at the early stage of growth. The cotton planted in the test group is shown on the right side of the figure, and the cotton planted in the control group is shown on the left side. As can be seen from the figure 1, when the multi-effect cotton seedling emergence nutrient composition provided by the embodiment of the invention is used for planting cotton, the cotton seedlings are quick and neat, the root systems are developed, the seedlings are complete and strong, the seedlings grow up and are strong, the stems are thick, the red and green parts are half, the leaves are flat, and the leaves are green. The root system is developed, the main root is deeply pricked, the lateral roots are widely distributed, and the seedling is not deficient and has no diseases.
In conclusion, the multi-effect cotton seedling emergence nutrient composition for the saline-alkali soil provided by the embodiment of the invention has the following advantages:
according to the multi-effect cotton seedling emergence nutrient composition for the saline-alkali land, provided by the embodiment of the invention, saline-alkali tolerant trichoderma harzianum can tolerate salt stress below 80g/L and alkali stress below pH8.0, has high activity in the saline-alkali land, can effectively inhibit and prevent plant diseases and insect pests, and has the effect of promoting root system growth. Bacillus thuringiensis produces two major toxins, namely endotoxin (parasporal crystals) and exotoxin, which causes pests to stop eating and finally die due to hunger and poisoning. Has obvious effect of preventing and controlling various pests of orthoptera, coleoptera, diptera and hymenoptera, particularly lepidoptera. The Streptomyces rochei enables seed inoculation to have antagonistic action, and the Streptomyces rochei metabolizes to generate enzyme and antibiotic for preventing pathogenic bacteria from invading, so that a protective layer is formed around the plant root to inhibit the propagation of the pathogenic bacteria infected firstly. Three kinds of beneficial bacteria of saline-alkali resistant trichoderma harzianum, bacillus thuringiensis and streptomyces rochei are combined for use, so that the synergistic insect pest prevention and control effect is realized, and the synergistic effect is shown by proper proportion. The compound amino acid, the biochemical potassium fulvate and the auxiliary materials timely balance inorganic and organic nutrients for cotton seedling growth, and the robust growth of cotton is promoted. Solves the problem of abnormal growth of cotton seedlings caused by unbalanced nutrition of soil. Meanwhile, the biochemical potassium fulvate can also induce the cold resistance, disease resistance and drought resistance of cotton, and improve the cold resistance, drought resistance and saline-alkali resistance of cotton. Solves the problem of harm to cotton seedling caused by low temperature and cold in late spring. The multi-effect cotton seedling emergence nutrient composition for the saline-alkali soil, provided by the embodiment of the invention, has the advantages of no environmental pollution, safety in use, capability of remarkably promoting germination and growth of vegetable seedlings, enhancement of stress resistance and promotion of crop yield increase.
The preparation method of the saline-alkali land cotton seedling emergence multi-effect nutritional composition provided by the embodiment of the invention has the advantages of simple processing technology, long storage period, low cost and suitability for large-scale production, and the saline-alkali land cotton nutritional composition obtained by production has comprehensive nutrition, can supply nutrition required by the cotton in the whole growth period in a balanced manner, and can enhance the disease resistance and stress resistance of plants and condition soil.
The application method of the multi-effect nutrient composition for cotton seedling emergence in saline-alkali land provided by the invention provides proper nutrients for the growth of cotton in saline-alkali land, reasonably controls fertilization and ensures that the cotton grows stably and robustly.
The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present invention shall be included.