CN112876304A - Composite microbial fertilizer and preparation method and application thereof - Google Patents
Composite microbial fertilizer and preparation method and application thereof Download PDFInfo
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- CN112876304A CN112876304A CN202110269068.XA CN202110269068A CN112876304A CN 112876304 A CN112876304 A CN 112876304A CN 202110269068 A CN202110269068 A CN 202110269068A CN 112876304 A CN112876304 A CN 112876304A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
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Abstract
The invention discloses a composite microbial fertilizer and a preparation method and application thereof, wherein the microbial fertilizer comprises the following raw materials in parts by weight: 0.1-20 parts of trichoderma harzianum, 0.1-10 parts of bacillus mucilaginosus, 0.1-30 parts of bacillus subtilis, 5-30 parts of amino acid, 1-30 parts of protein powder, 1-10 parts of glucose powder, 0.5-5 parts of organic acid and 1-10 parts of soybean meal. The microbial fertilizer disclosed by the embodiment of the invention is nontoxic, safe and instant, and can be manually sprayed, mixed with soil for broadcast application or aerial spraying by an unmanned aerial vehicle after being dissolved. The microbial fertilizer provided by the embodiment of the invention can be suitable for various crops.
Description
Technical Field
The invention relates to the technical field of fertilizer preparation, in particular to a compound microbial fertilizer and a preparation method and application thereof.
Background
The microbial fertilizer refers to a kind of specially manufactured fertilizer containing active microorganisms, and can obtain a specific fertilizer effect when being applied to agricultural production. The microbial fertilizer increases the supply of plant nutrient elements through the life activities of microorganisms contained in the microbial fertilizer, thereby improving the plant nutrient condition and promoting the yield to be increased; the crop can be increased by the action of life activities contained in the crops, but the crops are not limited to increasing the supply level of plant nutrient elements, but also comprise the stimulation effect of secondary metabolites, such as hormone substances, generated by the crops on plants, the absorption effect of the plants on the nutrient elements is promoted, or the pathogenic effect of certain pathogenic microorganisms can be antagonized, and the crop yield is increased by alleviating pests.
However, the existing microbial fertilizers have insufficient effects on improving the stress resistance of plants, even do not have the effects of improving the stress resistance of crops, such as enhancing the drought resistance and cold resistance of crops and preventing diseases, and further improvement is urgently needed.
Disclosure of Invention
Therefore, the invention provides a compound microbial fertilizer and a preparation method and application thereof.
In order to achieve the above purpose, the invention provides the following technical scheme:
the embodiment of the invention provides a composite microbial fertilizer which comprises the following raw materials in parts by weight: 0.1-20 parts of trichoderma harzianum, 0.1-10 parts of bacillus mucilaginosus, 0.1-30 parts of bacillus subtilis, 5-30 parts of amino acid, 1-30 parts of protein powder, 1-10 parts of glucose powder, 0.5-5 parts of organic acid and 1-10 parts of soybean meal.
In one embodiment of the invention, the fertilizer comprises the following raw materials in parts by weight: 1-19 parts of trichoderma harzianum, 1-9 parts of bacillus mucilaginosus, 1-29 parts of bacillus subtilis, 6-29 parts of amino acid, 2-29 parts of protein powder, 2-9 parts of glucose powder, 1-4 parts of organic acid and 2-9 parts of soybean meal.
In one embodiment of the invention, the fertilizer comprises the following raw materials in parts by weight: 10 parts of trichoderma harzianum, 5 parts of bacillus mucilaginosus, 15 parts of bacillus subtilis, 18 parts of amino acid, 18 parts of protein powder, 5 parts of glucose powder, 2 parts of organic acid and 5 parts of soybean meal.
In one embodiment of the invention, the fertilizer further comprises 1-10 parts by mass of a trace element fertilizer;
the trace element fertilizer comprises the following components in percentage by mass: 1-3 parts of calcium superphosphate, 1-2 parts of magnesium sulfate, 1-3 parts of zinc sulfate and 1-2 parts of boric acid.
The embodiment of the invention also provides a method for preparing the composite microbial fertilizer, which comprises the following steps:
taking Trichoderma harzianum, Bacillus mucilaginosus, Bacillus subtilis, amino acid, protein powder, glucose powder, organic acid and soybean meal according to the proportion;
crushing the amino acid, the protein powder, the glucose powder, the organic acid and the soybean powder to over 600 meshes to obtain mixed material powder;
taking 30% of the mixture powder by mass, and fully and uniformly mixing the mixture powder with trichoderma harzianum, bacillus mucilaginosus and bacillus subtilis to obtain a first mixed bacterial fertilizer;
fully and uniformly mixing the first mixed bacterial fertilizer and 50% of the mixed powder by mass to obtain a second mixed bacterial fertilizer;
and fully mixing the second mixed bacterial fertilizer with 20% of the mixed powder by mass to obtain the compound microbial fertilizer.
In one embodiment of the invention, the trichoderma harzianum has an effective viable count of 50 hundred million/gram;
the effective viable count of the bacillus mucilaginosus is 20 hundred million/gram;
the effective viable count of the bacillus subtilis is 20 hundred million/gram.
In one embodiment of the invention, the compound microbial fertilizer further comprises 1-10 parts of trace element fertilizer by mass.
In one embodiment of the invention, the trace element fertilizer comprises the following components in percentage by mass: 1-3 parts of calcium superphosphate, 1-2 parts of magnesium sulfate, 1-3 parts of zinc sulfate and 1-2 parts of boric acid.
The microbial fertilizer is applied to any one of (1) preparation of a product for improving the cold resistance of plants; (2) the product for improving the drought resistance of the plant is prepared, and also belongs to the protection scope of the invention.
The invention has the following advantages:
the microbial fertilizer disclosed by the embodiment of the invention is nontoxic, safe and instant, and can be manually sprayed, mixed with soil for broadcast application or aerial spraying by an unmanned aerial vehicle after being dissolved. The microbial fertilizer disclosed by the embodiment of the invention is applied to soil or a paddy field or sprayed to plant leaf surfaces after being coated with kernels, has strong reproductive capacity and high activity, and is 5-10 times of that of common biological bacteria. Can effectively improve the stress resistance of various crops and improve the drought resistance and cold resistance. Can effectively prevent various diseases of crops. Can quickly grow roots, the root system is developed, and the seedlings are uniform and strong. The microbial fertilizer provided by the embodiment of the invention can be suitable for various crops.
Detailed Description
The present invention is described in terms of particular embodiments, other advantages and features of the invention will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the invention and that it is not intended to limit the invention to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the examples of the present invention, the species Trichoderma harzianum, Bacillus mucilaginosus and Bacillus subtilis were purchased from Guangzhou Korea Biotech Co.
Example 1
The invention provides a compound microbial fertilizer which comprises the following raw materials in parts by mass: 0.1g of trichoderma harzianum, 0.1g of bacillus mucilaginosus, 0.1g of bacillus subtilis, 5g of amino acid, 1g of protein powder, 1g of glucose powder, 0.5g of organic acid, 1g of soybean meal and 1g of trace element fertilizer.
Wherein the microelement fertilizer comprises the following components in parts by mass: 1g of calcium superphosphate, 1g of magnesium sulfate, 1g of zinc sulfate and 1g of boric acid.
Example 2
The invention provides a compound microbial fertilizer which comprises the following raw materials in parts by mass: 10g of trichoderma harzianum, 5g of bacillus mucilaginosus, 15g of bacillus subtilis, 18g of amino acid, 18g of protein powder, 5g of glucose powder, 2g of organic acid, 5g of soybean meal and 5g of trace element fertilizer.
Wherein the microelement fertilizer comprises the following components in parts by mass: 2g of calcium superphosphate, 1.5g of magnesium sulfate, 2g of zinc sulfate and 1.5g of boric acid.
Example 3
The invention provides a compound microbial fertilizer which comprises the following raw materials in parts by mass: 20g of trichoderma harzianum, 10g of bacillus mucilaginosus, 30g of bacillus subtilis, 30g of amino acid, 30g of protein powder, 10g of glucose powder, 5g of organic acid, 10g of soybean meal and 10g of trace element fertilizer.
The trace element fertilizer comprises the following components in percentage by mass: 3g of calcium superphosphate, 2g of magnesium sulfate, 3g of zinc sulfate and 2g of boric acid.
Example 4
The embodiment of the invention provides a method for preparing a composite microbial fertilizer, which comprises the following steps:
taking Trichoderma harzianum, Bacillus mucilaginosus, Bacillus subtilis, amino acid, protein powder, glucose powder, organic acid and soybean meal according to the proportion; wherein the effective viable count of Trichoderma harzianum is 50 hundred million/g, the effective viable count of Bacillus mucilaginosus is 20 hundred million/g, and the effective viable count of Bacillus subtilis is 20 hundred million/g.
Pulverizing amino acids, protein powder, glucose powder, organic acid, and soybean powder to over 600 mesh with superfine pulverizer to obtain mixed powder;
taking 30% of the mixture powder by mass, and fully and uniformly mixing the mixture powder with the trichoderma harzianum, the bacillus mucilaginosus and the bacillus subtilis to obtain a first mixed bacterial fertilizer;
fully and uniformly mixing the first mixed bacterial fertilizer and 50% of the mixture powder by mass to obtain a second mixed bacterial fertilizer;
and fully mixing the second mixed bacterial fertilizer with 20% of the mixed powder by mass to obtain the compound microbial fertilizer. Through the full mixing of the three steps, the microorganism strains including trichoderma harzianum, bacillus mucilaginosus and bacillus subtilis are fully wrapped in various nutrients, and the strains are wrapped and carry a large amount of culture medium, and after entering soil or being sprayed on the leaf surfaces of crops, the propagation speed of the strains is fast, the density of the strains is high, and the activity of the strains is enhanced and is 5-10 times of that of common biological bacteria.
As a changeable implementation mode, the compound microbial fertilizer also comprises 1-10 parts of trace element fertilizer by mass; the trace element fertilizer comprises the following components in parts by weight: 1-3 parts of calcium superphosphate, 1-2 parts of magnesium sulfate, 1-3 parts of zinc sulfate and 1-2 parts of boric acid.
Test example 1
Designing a field test, sowing in the last ten days of 9 months, wherein the row spacing is 30cm, the row spacing of the final singling is 40cm, the fertilizer is used for 100 kg/mu, and the field management is carried out conventionally. The zones were divided, and the microbial fertilizers of examples 1 to 3 were applied, respectively, while the control group was not applied with the microbial fertilizer.
The test results are shown in table 1.
TABLE 1
| Plant density | Stress resistance | Drought resistance | Increase production | |
| Example 1 | 5500 | Disease-resistant, drought-resistant and cold-resistant | Strong ability of promoting rooting and drought resistance | 10％ |
| Example 2 | 5500 | Disease-resistant, drought-resistant and cold-resistant | Strong ability of promoting rooting and drought resistance | 15％ |
| Example 3 | 5500 | Disease-resistant, drought-resistant and cold-resistant | Strong ability of promoting rooting and drought resistance | 20％ |
| Control group | 5500 | Poor drought and cold resistance | Weak ability to promote rooting and drought resistance | 0 |
Test example 2
The microbial fertilizer product prepared in example 1 was applied to the rice seedbed: in the rice field, 10g of fertilizer is applied per square meter, seedlings are aligned and strong, and yield is increased by 10% after autumn;
in a rice field, 20g of the microbial fertilizer prepared in the example 1 is applied to each square meter of rice, seedlings are uniform, and yield is increased by 15% after autumn;
in a rice field, 30g of the microbial fertilizer prepared in the example 1 per square meter increases yield by 20 percent after seedling alignment, strong autumn
Spraying the microbial fertilizer product prepared in example 1 in a soybean field: the yield is increased by more than 10 percent by 6 kilograms per mu, 10 kilograms per mu and more than 22 percent.
Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (9)
1. The composite microbial fertilizer is characterized by comprising the following raw materials in parts by mass: 0.1-20 parts of trichoderma harzianum, 0.1-10 parts of bacillus mucilaginosus, 0.1-30 parts of bacillus subtilis, 5-30 parts of amino acid, 1-30 parts of protein powder, 1-10 parts of glucose powder, 0.5-5 parts of organic acid and 1-10 parts of soybean meal.
2. The composite microbial fertilizer of claim 1,
the fertilizer comprises the following raw materials in parts by weight: 1-19 parts of trichoderma harzianum, 1-9 parts of bacillus mucilaginosus, 1-29 parts of bacillus subtilis, 6-29 parts of amino acid, 2-29 parts of protein powder, 2-9 parts of glucose powder, 1-4 parts of organic acid and 2-9 parts of soybean meal.
3. The composite microbial fertilizer of claim 1,
the fertilizer comprises the following raw materials in parts by weight: 10 parts of trichoderma harzianum, 5 parts of bacillus mucilaginosus, 15 parts of bacillus subtilis, 18 parts of amino acid, 18 parts of protein powder, 5 parts of glucose powder, 2 parts of organic acid and 5 parts of soybean meal.
4. The composite microbial fertilizer according to claim 1,
the fertilizer also comprises 1-10 parts of trace element fertilizer by mass;
the trace element fertilizer comprises the following components in percentage by mass: 1-3 parts of calcium superphosphate, 1-2 parts of magnesium sulfate, 1-3 parts of zinc sulfate and 1-2 parts of boric acid.
5. A method of preparing the composite microbial fertilizer of any one of claims 1-3, comprising:
taking Trichoderma harzianum, Bacillus mucilaginosus, Bacillus subtilis, amino acid, protein powder, glucose powder, organic acid and soybean meal according to the proportion;
crushing the amino acid, the protein powder, the glucose powder, the organic acid and the soybean powder to over 600 meshes to obtain mixed material powder;
taking 30% of the mixture powder by mass, and fully and uniformly mixing the mixture powder with trichoderma harzianum, bacillus mucilaginosus and bacillus subtilis to obtain a first mixed bacterial fertilizer;
fully and uniformly mixing the first mixed bacterial fertilizer and 50% of the mixed powder by mass to obtain a second mixed bacterial fertilizer;
and fully mixing the second mixed bacterial fertilizer with 20% of the mixed powder by mass to obtain the compound microbial fertilizer.
6. The method of claim 5,
the effective viable count of the trichoderma harzianum is 50 hundred million/gram;
the effective viable count of the bacillus mucilaginosus is 20 hundred million/gram;
the effective viable count of the bacillus subtilis is 20 hundred million/gram.
7. The method of claim 5,
the compound microbial fertilizer also comprises 1-10 parts of trace element fertilizer by mass.
8. The method of claim 7,
the trace element fertilizer comprises the following components in percentage by mass: 1-3 parts of calcium superphosphate, 1-2 parts of magnesium sulfate, 1-3 parts of zinc sulfate and 1-2 parts of boric acid.
9. Use of the microbial fertilizer of any one of claims 1-4 in any one of,
(1) preparing a product for improving the cold resistance of plants;
(2) preparing the product for improving the drought resistance of the plants.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114804981A (en) * | 2022-04-18 | 2022-07-29 | 四平市圣星生物科技有限公司 | Biological organic fertilizer and preparation method thereof |
| CN115336517A (en) * | 2022-09-06 | 2022-11-15 | 昆明理工大学 | Ecological restoration plant growth bag for ultrahigh altitude area and use method thereof |
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| US20040151698A1 (en) * | 2000-05-02 | 2004-08-05 | Young-Ryun Chung | Microbial pesticide active against plant fungal pathogens and process for preparation thereof |
| CN103910578A (en) * | 2014-04-04 | 2014-07-09 | 山东丰本生物科技股份有限公司 | Rooting nutritional type microbial fertilizer, and preparation method and applications thereof |
| CN104671960A (en) * | 2013-11-29 | 2015-06-03 | 大连三科生物工程有限公司 | Amino acid microbial fertilizer |
| CN109956825A (en) * | 2019-04-30 | 2019-07-02 | 韩春斌 | A kind of multi-functional plant fertilizer for increasing production and preparation method thereof |
| CN111269063A (en) * | 2020-04-08 | 2020-06-12 | 邯郸市万惠生物技术有限公司 | Composite water-soluble fertilizer for tomatoes and preparation method thereof |
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2021
- 2021-03-12 CN CN202110269068.XA patent/CN112876304A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040151698A1 (en) * | 2000-05-02 | 2004-08-05 | Young-Ryun Chung | Microbial pesticide active against plant fungal pathogens and process for preparation thereof |
| CN104671960A (en) * | 2013-11-29 | 2015-06-03 | 大连三科生物工程有限公司 | Amino acid microbial fertilizer |
| CN103910578A (en) * | 2014-04-04 | 2014-07-09 | 山东丰本生物科技股份有限公司 | Rooting nutritional type microbial fertilizer, and preparation method and applications thereof |
| CN109956825A (en) * | 2019-04-30 | 2019-07-02 | 韩春斌 | A kind of multi-functional plant fertilizer for increasing production and preparation method thereof |
| CN111269063A (en) * | 2020-04-08 | 2020-06-12 | 邯郸市万惠生物技术有限公司 | Composite water-soluble fertilizer for tomatoes and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114804981A (en) * | 2022-04-18 | 2022-07-29 | 四平市圣星生物科技有限公司 | Biological organic fertilizer and preparation method thereof |
| CN115336517A (en) * | 2022-09-06 | 2022-11-15 | 昆明理工大学 | Ecological restoration plant growth bag for ultrahigh altitude area and use method thereof |
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