CN112500223A - Biological organic-inorganic fertilizer suitable for safflower large golden elements and preparation method thereof - Google Patents
Biological organic-inorganic fertilizer suitable for safflower large golden elements and preparation method thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses a biological organic inorganic fertilizer suitable for safflower large golden elements and a preparation method thereof, wherein the biological organic inorganic fertilizer comprises the following raw materials in parts by weight: 200-260 parts of biological organic fertilizer, 92-145 parts of monoammonium phosphate, 332 parts of potassium sulfate 235-332 parts of potassium nitrate, 45-156 parts of nitric phosphate fertilizer, 50-130 parts of calcium magnesium phosphate fertilizer, 40-50 parts of diammonium phosphate, 0-10 parts of sodium borate decahydrate, 0-40 parts of magnesium sulfate heptahydrate and 0-10 parts of zinc sulfate heptahydrate; the invention has the beneficial effects that: the functional strains of the fertilizer can be colonized in soil to form dominant flora, the bacillus subtilis has the effect of preventing and controlling black shank, the bacillus amyloliquefaciens has the effect of preventing and controlling bacterial wilt, the bacillus mucilaginosus has the effect of potassium dissolving and growth promotion, and all production raw materials are harmless, green and environment-friendly.
Description
Technical Field
The invention relates to the field of biological organic fertilizers, in particular to a biological organic-inorganic fertilizer suitable for safflower large golden elements and a preparation method thereof.
Background
Hongdaojinyuan original name Lu Mei Yi Yan, selected from Dajinyuan variant strain by Lu Mei Yi village Nicotiana, Lunan county, Yunnan province in 1962, and further selected and cultivated by flue-cured tobacco research institute of agriculture academy of sciences of Yunnan province and tobacco office of Qujing district in 1972-.
The safflower Dajinyuan has more advantages, has very typical faint scent type style after being made into cigarettes, and is always the main raw material of middle-high-end cigarettes. However, the variety is susceptible to black stem disease and scab, is difficult to bake and has a low yield.
Disclosure of Invention
The invention aims to provide a biological organic inorganic fertilizer suitable for safflower large golden dollars and a preparation method thereof aiming at the defects of the prior art.
In order to achieve the above object, the present invention is achieved by the following technical solutions.
A biological organic inorganic fertilizer suitable for safflower large golden elements comprises the following raw materials in parts by weight: 200-260 parts of biological organic fertilizer, 92-145 parts of monoammonium phosphate, 332 parts of potassium sulfate 235-332 parts of potassium nitrate, 45-156 parts of nitric phosphate fertilizer, 50-130 parts of calcium magnesium phosphate fertilizer, 40-50 parts of diammonium phosphate, 0-10 parts of sodium borate decahydrate, 0-40 parts of magnesium sulfate heptahydrate and 0-10 parts of zinc sulfate heptahydrate;
the biological organic fertilizer comprises an organic fertilizer and functional microbial inoculum fermentation liquor which occupies 1 per mill to 5 per mill of the weight of the organic fertilizer;
the organic fertilizer is rapeseed cake, and the functional microbial inoculum fermentation liquor is prepared by compounding bacillus subtilis fermentation liquor, bacillus amyloliquefaciens fermentation liquor and bacillus mucilaginosus fermentation liquor according to the mass ratio of 0.5-1.5: 2-4;
the preservation registration number of the bacillus subtilis is CGMCC No. 20142;
the preservation registration number of the bacillus amyloliquefaciens is CGMCC No. 20146;
the preservation registration number of the bacillus mucilaginosus is CGMCC No. 20152.
The preparation method comprises the following steps:
firstly, fermenting and decomposing organic fertilizer: squeezing natural rapeseed dregs to obtain a production raw material of an organic fertilizer, adding a fermentation microbial inoculum with the weight percentage of 0.3-0.5% of the raw material, uniformly mixing, adjusting the water content of the material to 45-65%, carrying out aerobic fermentation for 20-28 days, and completing the decomposition of the material to obtain the organic fertilizer;
step two, preparing a functional microbial inoculum: respectively activating bacillus subtilis, bacillus amyloliquefaciens and bacillus mucilaginosus to prepare seed solutions, respectively carrying out aerobic fermentation in fermentation tanks, culturing for 48-60 h under the conditions that the fermentation temperature is 35-38 ℃ and the rotation speed is 120rpm/min to respectively obtain strain fermentation liquids, and then compounding the three kinds of strain fermentation liquids to obtain functional microbial inoculum fermentation liquids;
step three, adding a functional microbial inoculum: adding the fermentation liquor of the functional microbial inoculum obtained in the second step into the organic fertilizer obtained in the first step according to the weight ratio of 1-5 per mill, and uniformly mixing to obtain a biological organic fertilizer;
fourthly, preparing the biological-organic-inorganic fertilizer: mixing the biological organic fertilizer obtained in the third step with inorganic raw materials according to a proportion to obtain a biological-organic-inorganic fertilizer;
fifthly, product packaging: drying, metering and packaging the biological-organic-inorganic fertilizer obtained in the fourth step to obtain a powdery biological-organic-inorganic fertilizer product; and (4) granulating, drying, screening, metering and packaging the biological-organic-inorganic fertilizer obtained in the fourth step to obtain a granular biological-organic-inorganic fertilizer product.
Further, the fermentation culture solution of the strains in the fermentation tank in the second step comprises the following components in parts by weight: 2-4 parts of soybean meal, 0.5-1 part of cane sugar, 1-3 parts of beef extract, 2-3 parts of corn flour, 0.8-1.5 parts of calcium carbonate, 1-2 parts of fish meal, 0.05-0.1 part of potassium dihydrogen phosphate, 0.05-0.1 part of dipotassium hydrogen phosphate, 1-5 parts of sodium chloride and 0.1-0.3 part of defoaming agent, wherein the pH is adjusted by hydrochloric acid and sodium hydroxide to be 6.8-7.5.
Furthermore, the effective viable count of the strain fermentation liquor in the second step is more than 2.0 multiplied by 1010cfu/g。
The invention has the beneficial effects that: the oil cake used in the fertilizer raw materials is the cake after pressing rapeseed, the inorganic raw materials are selected to meet the production requirements of ecological fertilizers, functional strains can be colonized in soil to form dominant flora, the bacillus subtilis has the effect of preventing and controlling black shank, the bacillus amyloliquefaciens has the effect of preventing and controlling bacterial wilt, the bacillus mucilaginosus has the effect of decomposing potassium and promoting growth, all the production raw materials are harmless, green and environment-friendly, and meanwhile, the fertilizer formula is attached to the characteristics of low nutrient requirement and strong growth potential of Honghuadajinyuan, can meet the basic nutrients of the whole growth period of Honghuadajinyuan tobacco, and has great promotion effects on promoting the healthy growth, layered yellowing and mature picking and baking of the Honghuadajinyuan tobacco.
Detailed Description
The present invention is further illustrated by the following examples, but is not limited to the details of the description.
A biological organic inorganic fertilizer suitable for safflower large golden elements comprises the following raw materials in parts by weight:
200-260 parts of biological organic fertilizer, 92-145 parts of monoammonium phosphate, 332 parts of potassium sulfate 235-332 parts of potassium nitrate, 45-156 parts of nitric phosphate fertilizer, 50-130 parts of calcium magnesium phosphate fertilizer, 40-50 parts of diammonium phosphate, 0-10 parts of sodium borate decahydrate, 0-40 parts of magnesium sulfate heptahydrate and 0-10 parts of zinc sulfate heptahydrate; the biological organic fertilizer comprises an organic fertilizer and a functional microbial inoculum fermentation liquid accounting for 1-5 per mill of the weight of the organic fertilizer, wherein the organic fertilizer is rapeseed cake, and the functional microbial inoculum fermentation liquid is bacillus subtilis fermentation liquid, bacillus amyloliquefaciens fermentation liquid and bacillus mucilaginosus fermentation liquid which are compounded according to the mass ratio of 0.5-1.5: 2-4.
The preservation registration numbers of the Bacillus subtilis are CGMCC No.20142 respectively; the preservation date is as follows: 28/6/2020, depository: china general microbiological culture Collection center; and (4) storage address: xilu No. 1 Hospital No. 3, Beijing, Chaoyang, North.
The preservation registration number of the Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) is CGMCC No. 20146; the preservation date is as follows: 28/6/2020, depository: china general microbiological culture Collection center; and (4) storage address: xilu No. 1 Hospital No. 3, Beijing, Chaoyang, North.
The preservation registration number of the Bacillus mucilaginosus (Bacillus mucilaginosus) is CGM CC No. 20152; the preservation date is as follows: 28/6/2020, depository: china general microbiological culture Collection center; and (4) storage address: xilu No. 1 Hospital No. 3, Beijing, Chaoyang, North Chen.
The preparation method comprises the following steps:
firstly, fermenting and decomposing organic fertilizer: taking natural rapeseed cakes (called as flaky cakes and oil cakes) obtained by a squeezing method as production raw materials of the organic fertilizer, adding 0.3-0.5% of zymophyte agent according to the weight ratio for mixing, adjusting the water content of the materials to 45-65%, carrying out aerobic fermentation on the materials by adopting a full-automatic industrial production line, and completing the decomposition of the materials through a fermentation process of 20-28 days to obtain the organic fertilizer; the existing fermentation inoculum can be selected from the fermentation inoculum described in patent No. ZL201310055173.9, and has excellent effect on the fermentation of plant residues;
step two, preparing a functional microbial inoculum: respectively activating bacillus subtilis, bacillus amyloliquefaciens and bacillus mucilaginosus to prepare seed solutions, respectively carrying out aerobic fermentation in a fermentation tank, culturing for 48-60 h under the conditions that the temperature is 35-38 ℃ and the rotating speed is 120rpm/min to obtain functional strain fermentation liquor, and then compounding the three kinds of strain fermentation liquor according to the mass ratio of 0.5-1.5: 2-4 to obtain functional microbial inoculum fermentation liquor;
the bacillus subtilis has the effect of preventing and controlling black shank, the bacillus amyloliquefaciens has the effect of preventing and controlling bacterial wilt, and the bacillus mucilaginosus has the effect of decomposing potassium and promoting growth;
the effective viable count of the functional strain fermentation liquor is more than 2.0 multiplied by 1010cfu/g；
The components and the parts by weight of the strain fermentation culture solution in the fermentation tank are as follows: 2-4 parts of soybean powder, 0.5-1 part of cane sugar, 1-3 parts of beef extract, 2-3 parts of corn flour, 0.8-1.5 parts of calcium carbonate, 1-2 parts of fish meal, 0.05-0.1 part of potassium dihydrogen phosphate, 0.05-0.1 part of dipotassium hydrogen phosphate, 1-5 parts of sodium chloride and 0.1-0.3 part of defoaming agent, wherein the pH is adjusted by hydrochloric acid and sodium hydroxide to be 6.8-7.5;
step three, adding a functional microbial inoculum: and (3) adding the fermentation liquor of the functional microbial inoculum obtained in the second step into the organic fertilizer obtained in the first step according to the weight ratio of 1-5 per mill, and uniformly mixing to obtain the biological organic fertilizer.
Fourthly, preparing the biological-organic-inorganic fertilizer: and mixing the obtained biological organic fertilizer and the inorganic raw material according to a proportion to obtain the biological-organic-inorganic fertilizer.
Fifthly, product packaging: drying, metering and packaging the biological-organic-inorganic fertilizer obtained in the fourth step to obtain a powdery biological-organic-inorganic fertilizer product; and (4) granulating, drying, screening, metering and packaging the biological-organic-inorganic fertilizer obtained in the fourth step to obtain a granular biological-organic-inorganic fertilizer product.
The prepared biological-organic-inorganic fertilizer is suitable for planting Honghuadajinyuan tobacco leaves and can be applied to planting Honghuadajinyuan tobacco leaves and other tobacco varieties.
Detailed description of the preferred embodiment 1
A biological organic inorganic fertilizer suitable for safflower large golden elements comprises the following raw materials in parts by weight: 200 parts of bio-organic fertilizer, 145 parts of monoammonium phosphate, 235 parts of potassium sulfate, 135 parts of potassium nitrate, 45 parts of nitrophosphate fertilizer, 130 parts of calcium magnesium phosphate fertilizer, 50 parts of diammonium phosphate, 10 parts of sodium borate decahydrate, 40 parts of magnesium sulfate heptahydrate and 10 parts of zinc sulfate heptahydrate; the biological organic fertilizer comprises an organic fertilizer and functional microbial inoculum fermentation liquor which occupies 1 per mill of the weight of the organic fertilizer, wherein the organic fertilizer is rapeseed cake, and the functional microbial inoculum fermentation liquor is bacillus subtilis fermentation liquor, bacillus amyloliquefaciens fermentation liquor and bacillus mucilaginosus fermentation liquor which are compounded according to the mass ratio of 0.5: 1.5: 2.
Detailed description of the preferred embodiment 2
A biological organic inorganic fertilizer suitable for safflower large golden elements comprises the following raw materials in parts by weight: 260 parts of bio-organic fertilizer, 92 parts of monoammonium phosphate, 332 parts of potassium sulfate, 70 parts of potassium nitrate, 156 parts of nitrophosphate fertilizer, 50 parts of calcium-magnesia phosphate fertilizer, 40 parts of diammonium phosphate, 0 part of sodium borate decahydrate, 0 part of magnesium sulfate heptahydrate and 0 part of zinc sulfate heptahydrate; the biological organic fertilizer comprises an organic fertilizer and a functional microbial inoculum fermentation liquor which occupies 5 per mill of the weight of the organic fertilizer, wherein the organic fertilizer is rapeseed cake, the functional microbial inoculum fermentation liquor is bacillus subtilis fermentation liquor,
The bacillus amyloliquefaciens fermentation liquid and the bacillus mucilaginosus fermentation liquid are compounded according to the mass ratio of 1.5: 0.5: 4 to obtain the bacillus amyloliquefaciens fermentation liquid.
Specific example 3
A biological organic inorganic fertilizer suitable for safflower large golden elements comprises the following raw materials in parts by weight: 230 parts of biological organic fertilizer, 125 parts of monoammonium phosphate, 285 parts of potassium sulfate, 100 parts of potassium nitrate, 100 parts of nitrophosphate fertilizer, 90 parts of calcium magnesium phosphate fertilizer, 45 parts of diammonium phosphate, 5 parts of sodium borate decahydrate, 0 part of magnesium sulfate heptahydrate and 0 part of zinc sulfate heptahydrate; the biological organic fertilizer comprises an organic fertilizer and functional microbial inoculum fermentation liquor accounting for 3 per mill of the weight of the organic fertilizer, wherein the organic fertilizer is rapeseed cake, and the functional microbial inoculum fermentation liquor is bacillus subtilis fermentation liquor, bacillus amyloliquefaciens fermentation liquor and bacillus mucilaginosus fermentation liquor which are compounded according to the mass ratio of 1: 3.
Specific example 4
A biological organic inorganic fertilizer suitable for safflower large golden elements comprises the following raw materials in parts by weight: 230 parts of biological organic fertilizer, 125 parts of monoammonium phosphate, 285 parts of potassium sulfate, 100 parts of potassium nitrate, 100 parts of nitrophosphate fertilizer, 90 parts of calcium magnesium phosphate fertilizer, 45 parts of diammonium phosphate, 5 parts of sodium borate decahydrate, 5 parts of magnesium sulfate heptahydrate and 0 part of zinc sulfate heptahydrate; the biological organic fertilizer comprises an organic fertilizer and functional microbial inoculum fermentation liquor accounting for 3 per mill of the weight of the organic fertilizer, wherein the organic fertilizer is rapeseed cake, and the functional microbial inoculum fermentation liquor is bacillus subtilis fermentation liquor, bacillus amyloliquefaciens fermentation liquor and bacillus mucilaginosus fermentation liquor which are compounded according to the mass ratio of 1: 3.
Specific example 5
A biological organic inorganic fertilizer suitable for safflower large golden elements comprises the following raw materials in parts by weight: 230 parts of biological organic fertilizer, 125 parts of monoammonium phosphate, 285 parts of potassium sulfate, 100 parts of potassium nitrate, 100 parts of nitrophosphate fertilizer, 90 parts of calcium magnesium phosphate fertilizer, 45 parts of diammonium phosphate, 5 parts of sodium borate decahydrate, 5 parts of magnesium sulfate heptahydrate and 5 parts of zinc sulfate heptahydrate; the biological organic fertilizer comprises an organic fertilizer and functional microbial inoculum fermentation liquor accounting for 3 per mill of the weight of the organic fertilizer, wherein the organic fertilizer is rapeseed cake, and the functional microbial inoculum fermentation liquor is bacillus subtilis fermentation liquor, bacillus amyloliquefaciens fermentation liquor and bacillus mucilaginosus fermentation liquor which are compounded according to the mass ratio of 1: 3.
The fertilizer efficiency of the fertilizer prepared in this example was used for the following potting experiment
Test site
The test is arranged in a greenhouse of Yunnan leaf chemical fertilizer limited company of Qinglong town, Anning, and soil with medium fertility and uniform fertility distribution is selected for pot culture test.
Test materials, test varieties: safflower Dajinyuan;
fertilizer to be tested: experiments 2-6 are the fertilizers prepared in examples 1-5, respectively, and experiment 1 is a commercial bio-organic-inorganic fertilizer (e.g., Yunye Compound fertilizers 8-16-26)
The test set 6 treatments, each of 10 pots, 2 cigarettes per pot, the test treatments and the amount of applied fertilizer were as follows:
TABLE 1 test of fertilizer dosage for each treatment
The fertilizer is placed in a greenhouse according to the test treatment sequence, so that the consistency of the illumination, the temperature, the soil water content and the watering amount of each test treatment is ensured, and the production management measures are consistent except for different fertilizer application in each treatment.
Table 2 test growth period of each treatment
Agronomic trait survey
TABLE 3 agronomic trait survey record
Raw tobacco appearance quality observation
TABLE 4 original cigarette appearance quality recording table
| Processing code number | Degree of maturity | Colour(s) | Gloss of | Oil content | Single leaf weight |
| T1 | Well done | High strength | Is slightly provided with | Is provided with | 38g |
| T2 | Well done | High strength | Is slightly provided with | Is provided with | 60g |
| T3 | Well done | High strength | Is slightly provided with | Is provided with | 61g |
| T4 | Well done | High strength | Is slightly provided with | Is provided with | 72g |
| T5 | Well done | High strength | Is slightly provided with | Is provided with | 63g |
| T6 | Well done | High strength | Is slightly provided with | Is provided with | 63g |
TABLE 5 biological quantity determination
Through analysis of test data, it can be seen that addition of organic matters contributes to dry matter accumulation of leaves at the later stage of tobacco, inorganic and organic nutrients are jointly used by workers of the compound fertilizer, great help is provided for improvement of yield and output of tobacco leaves, and meanwhile, coordination of nutrient absorption and overall growth of tobacco can be promoted through addition of medium trace elements.
The following field test was conducted on disease resistance of the fertilizer prepared in example
First, purpose and meaning of experiment
Honghuadajinyuan is one of the flue-cured tobacco varieties mainly planted in Yunnan province for years. The popularization of the variety is influenced to a certain extent due to poor resistance in practical production. The Honghuadajinyuan can resist south root knot nematode disease, has mild climatic spot disease, is infected with brown spot disease and black shank disease, and is infected with wild fire and common mosaic disease. Wherein, the main diseases of tobacco black shank, root black rot, bacterial wilt, soft rot, root knot nematode disease and the like can occur in the seedbed and the field period, which often generates huge loss to tobacco planting. The experiment aims to apply a biological organic fertilizer prepared by combining a sexual functional microbial agent with organic and inorganic nutrients during tobacco transplantation, and tries to effectively control the occurrence of common diseases such as tobacco black shank, root black rot, bacterial wilt, soft rot and root knot nematode; meanwhile, the growth of plant root systems is promoted, and the nutrient utilization rate is improved so as to provide technical support for preventing and treating common diseases of the large golden elements of the safflower.
Materials and methods
2.1. Location and variety of implementation
The tobacco in the lake and south generally bridges a representative tobacco planting area of a female river bed unit adjacent to the lake, the test land requires medium fertility, the fertility is uniformly distributed, the illumination and irrigation conditions are good, and the test of the fertilizer is not carried out in nearly two years, so that the test land can represent the land parcel of the main soil type in the area. The main local cultivated variety Honghuadajinyuan is used as a test variety.
2.2 test materials
10-8-20 parts of tobacco special fertilizer cloud leaves used in local tobacco production, and the biological organic and inorganic fertilizer of the embodiments 1-5 of the invention.
2.3 design of the experiment
The cell test is divided into 2 processes, which are respectively as follows:
process 1 (CK): the amount of the special base fertilizer for tobacco is determined according to the production standard of local high-quality tobacco leaves.
Treatment 2-6: the bio-organic-inorganic fertilizers of examples 1-5 of the present invention were reduced by 0.5 pure nitrogen amount in comparison with the amount used.
Each treatment is repeated for 3 times, 6 cells are totally formed, each cell is a 66 square meter, and the row spacing is implemented according to the production standard of local high-quality tobacco leaves. The cells are arranged in random blocks.
In order to ensure the application effect of the biological fertilizer, pesticide and other medicines are not required to be sprayed as far as possible within three days before and after the fertilizer is applied, and other farming operations are the same as the production scheme of the local field high-quality tobacco leaves.
Third, test recording and observation
3.1 growth phase progression
As seen by the growth period, as in table 6, there was no very significant difference between the control and the test, and the bio-organic-inorganic fertilizer did not have a significant effect on the tobacco plant growth period.
TABLE 6 growth stage progression
From the above table 6, under the condition of the same transplanting time, the time difference between the treatment entering the mass stage and the treatment entering the vigorous growth stage is only 1 day before and after, and the growth stage time is basically the same.
3.2 Pest investigation
And (4) investigating the occurrence condition of the diseases and insect pests of each treatment, and judging the prevention and control conditions of the biological-organic-inorganic fertilizer on the diseases such as blackleg and bacterial wilt and the like which are frequently generated by Honghuadajinyuan.
TABLE 7 field disease Condition
In the field of the year, except for sunscald phenomenon caused by strong light, the tobacco plants mainly have black shank and bacterial wilt in the later growth period of the tobacco plants, as can be seen from the field disease conditions in the table 2, the incidence of the bacterial wilt and the black shank treated by 2-6 is obviously lower than that of the bacterial wilt and the bacterial wilt treated by 1, but the control measures are not carried out by the farmers in the control area in advance, and the chemical agents are used only after the tobacco plants are found to be infected by the diseases, which indicates that the biological control measures have the effect of preventing the bacterial wilt and the black shank. Treatment 2 showed lower disease rates than treatment 1, indicating that tobacco plants using the bio-organic-inorganic fertilizer of the present invention had higher disease resistance than tobacco plants using a fertilizer specific to tobacco.
It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications in light of the above teachings may occur to those skilled in the art. Not all embodiments are exhaustive. All obvious changes and modifications which are obvious to the technical scheme of the invention are covered by the protection scope of the invention.
Claims (4)
1. A biological organic-inorganic fertilizer suitable for safflower large golden dollars is characterized in that: the composite material comprises the following raw materials in parts by weight:
200-260 parts of biological organic fertilizer, 92-145 parts of monoammonium phosphate, 332 parts of potassium sulfate 235-332 parts of potassium nitrate, 45-156 parts of nitric phosphate fertilizer, 50-130 parts of calcium magnesium phosphate fertilizer, 40-50 parts of diammonium phosphate, 0-10 parts of sodium borate decahydrate, 0-40 parts of magnesium sulfate heptahydrate and 0-10 parts of zinc sulfate heptahydrate;
the biological organic fertilizer comprises an organic fertilizer and functional microbial inoculum fermentation liquor which occupies 1 per mill to 5 per mill of the weight of the organic fertilizer;
the organic fertilizer is rapeseed cake, and the functional microbial inoculum fermentation liquor is obtained by compounding bacillus subtilis fermentation liquor, bacillus amyloliquefaciens fermentation liquor and bacillus mucilaginosus fermentation liquor according to the mass ratio of 0.5-1.5: 2-4;
the preservation registration number of the bacillus subtilis is CGMCC No. 20142;
the preservation registration number of the bacillus amyloliquefaciens is CGMCC No. 20146;
the preservation registration number of the bacillus mucilaginosus is CGMCC No. 20152.
2. The bio-organic-inorganic fertilizer suitable for Honghua Dajinyuan according to claim 1, wherein: the preparation method comprises the following steps:
firstly, fermenting and decomposing organic fertilizer: squeezing natural rapeseed dregs to obtain a production raw material of the organic fertilizer, adding a fermentation microbial inoculum with the weight percentage of 0.3-0.5% of the raw material, uniformly mixing, adjusting the water content of the material to 45-65%, carrying out aerobic fermentation for 20-28 days, and completing the decomposition of the material to obtain the organic fertilizer;
step two, preparing a functional microbial inoculum: respectively activating bacillus subtilis, bacillus amyloliquefaciens and bacillus mucilaginosus to prepare seed solutions, respectively carrying out aerobic fermentation in fermentation tanks, culturing for 48-60 h under the conditions that the fermentation temperature is 35-38 ℃ and the rotation speed is 120rpm/min to respectively obtain strain fermentation liquids, and then compounding the three kinds of strain fermentation liquids to obtain functional microbial inoculum fermentation liquids;
step three, adding a functional microbial inoculum: adding the fermentation liquor of the functional microbial inoculum obtained in the second step into the organic fertilizer obtained in the first step according to the weight ratio of 1-5 per mill, and uniformly mixing to obtain a biological organic fertilizer;
fourthly, preparing the biological-organic-inorganic fertilizer: mixing the biological organic fertilizer obtained in the third step with an inorganic raw material according to a ratio to obtain a biological-organic-inorganic fertilizer;
fifthly, product packaging: drying, metering and packaging the bio-organic-inorganic fertilizer obtained in the fourth step to obtain a powdery bio-organic-inorganic fertilizer product; and (4) granulating, drying, screening, metering and packaging the biological-organic-inorganic fertilizer obtained in the fourth step to obtain a granular biological-organic-inorganic fertilizer product.
3. The bio-organic-inorganic fertilizer suitable for Honghua Dajinyuan according to claim 2, wherein: the fermentation culture solution of the strains in the fermentation tank in the second step comprises the following components in parts by weight: 2-4 parts of soybean meal, 0.5-1 part of cane sugar, 1-3 parts of beef extract, 2-3 parts of corn flour, 0.8-1.5 parts of calcium carbonate, 1-2 parts of fish meal, 0.05-0.1 part of potassium dihydrogen phosphate, 0.05-0.1 part of dipotassium hydrogen phosphate, 1-5 parts of sodium chloride and 0.1-0.3 part of defoaming agent, wherein the pH is adjusted by hydrochloric acid and sodium hydroxide to be 6.8-7.5.
4. The bio-organic-inorganic fertilizer suitable for Honghua Dajinyuan according to claim 2, wherein: the effective viable count of the strain fermentation liquor in the second step is more than 2.0 multiplied by 1010cfu/g。
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112358997A (en) * | 2020-11-20 | 2021-02-12 | 云南省烟草公司曲靖市公司 | Bacterial strain with biocontrol function on tobacco brown spot, fermentation method of bacterial strain and tobacco brown spot prevention and control compound agent |
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| CN104119119A (en) * | 2014-07-14 | 2014-10-29 | 云南农业大学 | Special high-efficient water flush fertilizer for "Honghua Dajinyuan" flue-cured tobacco |
| CN110250209A (en) * | 2019-06-19 | 2019-09-20 | 云南省微生物发酵工程研究中心有限公司 | A kind of microbial bacterial agent and its preparation method and application with the mildew and rot sick function of prevention and treatment tobacco |
| CN111807898A (en) * | 2020-05-15 | 2020-10-23 | 云南省微生物发酵工程研究中心有限公司 | Bio-organic fertilizer with function of preventing and treating tobacco soil-borne diseases and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104119119A (en) * | 2014-07-14 | 2014-10-29 | 云南农业大学 | Special high-efficient water flush fertilizer for "Honghua Dajinyuan" flue-cured tobacco |
| CN110250209A (en) * | 2019-06-19 | 2019-09-20 | 云南省微生物发酵工程研究中心有限公司 | A kind of microbial bacterial agent and its preparation method and application with the mildew and rot sick function of prevention and treatment tobacco |
| CN111807898A (en) * | 2020-05-15 | 2020-10-23 | 云南省微生物发酵工程研究中心有限公司 | Bio-organic fertilizer with function of preventing and treating tobacco soil-borne diseases and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112358997A (en) * | 2020-11-20 | 2021-02-12 | 云南省烟草公司曲靖市公司 | Bacterial strain with biocontrol function on tobacco brown spot, fermentation method of bacterial strain and tobacco brown spot prevention and control compound agent |
| CN112358997B (en) * | 2020-11-20 | 2022-09-27 | 云南省烟草公司曲靖市公司 | Bacterial strain with biocontrol function on tobacco brown spot, fermentation method of bacterial strain and tobacco brown spot prevention and control compound agent |
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