CN103931626B - Containing the bactericidal composition of chitosan oligosaccharide - Google Patents
Containing the bactericidal composition of chitosan oligosaccharide Download PDFInfo
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- OMOVVBIIQSXZSZ-UHFFFAOYSA-N [6-(4-acetyloxy-5,9a-dimethyl-2,7-dioxo-4,5a,6,9-tetrahydro-3h-pyrano[3,4-b]oxepin-5-yl)-5-formyloxy-3-(furan-3-yl)-3a-methyl-7-methylidene-1a,2,3,4,5,6-hexahydroindeno[1,7a-b]oxiren-4-yl] 2-hydroxy-3-methylpentanoate Chemical compound CC12C(OC(=O)C(O)C(C)CC)C(OC=O)C(C3(C)C(CC(=O)OC4(C)COC(=O)CC43)OC(C)=O)C(=C)C32OC3CC1C=1C=COC=1 OMOVVBIIQSXZSZ-UHFFFAOYSA-N 0.000 description 2
- MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 description 2
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to plant protection art; relate to a kind of bactericidal composition containing chitosan oligosaccharide; it is characterized in that said composition contains A, B two kinds of components, component A is chitosan oligosaccharide, and B component is SYP-Zo48 or fenpiclonil; composition shows good synergistic effect in certain ratio range; improve the protection effect of composition, the multiple diseases in agricultural production can be prevented and treated, decrease access times; reduce use cost, delay pathogen to the drug-fast generation of single dose.
Description
Technical field
The invention belongs to plant protection art, relate to a kind of bactericidal composition containing chitosan oligosaccharide, composition is mainly used in preventing and treating gray mold, powdery mildew, banded sclerotial blight, bakanae disease, stalk break, fusarium wilt etc.
Background technology
In recent years, due to single and not Scientific Usage of Drugs, crop pest has produced pesticide resistance in various degree to medicament, and cause that peasant's cost improves, the destruction etc. of environmental pollution and ecotope.Being undertaken composite by the composition of two kinds of different structures, the different mechanism of action, is the effective way of minimizing and delaying drug resistance insect and raising drug effect.
SYP-Zo48, molecular formula is C
16h
17clN
2o, is belong to sterol synthesis inhibition bactericide, effectively can be controlled the occurrence and infection of disease on the ground by root application.There is the bactericidal activity of wide spectrum, there is protection and therapeutic action and good interior absorption simultaneously, the multiple diseases such as vegetables, Cereal, fruit tree can be prevented and treated.
Fenpiclonil, molecular formula is C
11h
6cl
2n
2, belong to pyroles protective fungicide, the main transfer suppressing glucose phosphorylated relevant, and the mycelial growth of Antifungi, finally cause germ dead, passes and soil-borne disease bacterium has good control efficiency to the kind of crop.
Also there is no the report about chitosan oligosaccharide and SYP-Zo48 or fenpiclonil composition research at present.Inventor, by the research to the bactericidal composition containing chitosan oligosaccharide, has found some beyond thought effects.
Summary of the invention
The object of the present invention is to provide the Synergistic bactericidal composition containing chitosan oligosaccharide, it is undertaken composite by the sterilization component of two kinds of different structures, the different mechanism of action, expanding prevention can compose and improve drug effect, the more important thing is and avoid or delay the generation of disease to new composition resistance.The object of the invention is to be realized by following measures.
The invention provides a kind of bactericidal composition containing chitosan oligosaccharide, it is characterized in that said composition contains A, B two kinds of components, component A is chitosan oligosaccharide, B component is SYP-Zo48 or fenpiclonil, ratio of quality and the number of copies between two components is 1:30-30:1, preferably, between two components, ratio of quality and the number of copies is 1:20-20:1.
In above-mentioned bactericidal composition, preferably, the quality sum of two components accounts for the 1-90% of bactericidal composition gross mass.
Above-mentioned composition of the present invention can be made into suspending agent, suspending agent seed coat agent, wet seed dressing, wetting powder, water dispersible granules, microemulsion etc., for dressing seed, filling with root, spray, mixing soil or spread fertilizer over the fields.
Present invention also offers above-mentioned composition for preventing and treating the purposes of crop pest, the disease of suitable control has the fungus-caused gray mold of Fungi Imperfecti, banded sclerotial blight, fusarium wilt etc., the fungus-caused powdery mildew of Ascomycotina, bakanae disease, stalk break etc., the banded sclerotial blight that Basidiomycotina causes, rust, smut etc., the fungus-caused downy mildew of Mastigomycotina, late blight etc.Therefore, the present invention also comprises the purposes of various agricultural fungal diseases on above-mentioned composition control crop.
The purposes of foregoing, preferably, the controlling disease of composition has gray mold, powdery mildew, fusarium wilt, bakanae disease, banded sclerotial blight, stalk break etc.
In the purposes of above-mentioned composition of the present invention, described crop is preferably tomato, cucumber, watermelon, paddy rice, wheat, rape, apple, grape, oranges and tangerines, pears, lichee, flowers etc.
In the present invention, chitosan oligosaccharide is Glucosamine is the mixture of the high molecular polymer of 2-30 by the degree of polymerization that Isosorbide-5-Nitrae-glycosidic bond is formed by connecting, and mean molecule quantity is 1000-3000 dalton.Chitosan oligosaccharide can be prepared by any means, such as following method: the shitosan of deacetylation more than 70% is the mixture of the chitosan oligomer of 2-30 by the degree of polymerization that the methods such as physical degradation methods, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation method prepare.Or, obtain chitin by extracting from fungal cell wall and be the mixture of the chitosan oligomer of 2-30 by the degree of polymerization that the methods such as physical degradation methods, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation method prepare.Or, by by Glucosamine by the degree of polymerization that Isosorbide-5-Nitrae-glycosidic bond chemosynthesis is polymerized being the mixture of the polymer of 2-30.Current chitosan oligosaccharide is the mixture of the high molecular polymer of different polymer, be not that is the oligosaccharides form of the single degree of polymerization in chitosan oligosaccharide, but the chitosan oligosaccharide monose of often kind of degree of polymerization all may exist.
Beneficial effect of the present invention is:
1, synergistic effect is obvious: composition shows good synergistic effect in certain ratio range, improves the protection effect of composition.
2, expanding prevention spectrum: composition can prevent and treat the multiple diseases in agricultural production.
3, use cost is reduced: because of composite, there is raising preventive effect, the increase of control spectrum, decrease access times, reduce use cost;
4, delay pesticide resistance: the composition of two kinds of different mechanism of action is composite, and pathogen not easily develops immunity to drugs, delay pathogen to the drug-fast generation of single dose.
Embodiment
Following examples further illustrate of the present invention, but the present invention is not limited to composition in present embodiment and purposes.
Embodiment 1 contains the bactericidal composition of chitosan oligosaccharide to the control efficiency of graw mold of tomato
Be mixed into compost with sterilized soil and sterilizing river sand, fully after mixing, be mixed into medicament.Drug concentration contains the milligram number of the active ingredient of this medicament in every kilogram of soil, directly mix soil, add clear water and make water content 30%, then mix rear quantitative loading basin alms bowl thoroughly after fully mixing thoroughly with corresponding quantitatively aqueous suspension formulations.Tomato is planted in basin alms bowl, Routine Management.The percentage that plant blade face gray mold infects is measured when blank gray mold generally occurs.Use Abbot formula, convert percentage to preventive effect.
Theoretical effect (%)=x+y-xy
The preventive effect of the active component A that x is working concentration when being a, the preventive effect of the active component B that y is working concentration when being b.Relatively theoretical effect and actual effect, evaluates the synergy of composition, for synergistic effect when being greater than theoretical preventive effect with actual preventive effect.The results are shown in Table 1.
Table 1 contains the bactericidal composition of chitosan oligosaccharide to the control efficiency of graw mold of tomato
By potted plant test, as seen from Table 1, the composition of the composition of chitosan oligosaccharide and SYP-Zo48, chitosan oligosaccharide and fenpiclonil to the actual effect of graw mold of tomato all higher than the theoretical preventive effect of composition.Obviously, the composition of the composition of chitosan oligosaccharide and SYP-Zo48, chitosan oligosaccharide and fenpiclonil is synergy to graw mold of tomato.
Embodiment 2 contains the bactericidal composition of chitosan oligosaccharide to the control efficiency of powdery mildew of cucumber
Cucumber seeds is seeded in be equipped with quantitatively, evenly, in the basin alms bowl of the consistent soil of moisture, Routine Management after emerging.Cucumber plant, substantially in 4 leaf phases, investigates powdery mildew incidence before the 1st medicine.With having carried out the sick pot experiment of powdery mildew of cucumber containing the bactericidal composition water dispersible granules of chitosan oligosaccharide, and establish corresponding single dose and blank, adopt the conventional conventional spray of manual sprayer, every medicament sprays 10 strains, and the 7-10d after 1 time that sprays sprays 1 time again.10-14d investigation after 1st dispenser, whole blade is investigated in every strain, and record investigation total number of sheets, the sick number of sheets, calculate disease index and preventive effect according to following stage division.
Blade staging:
0 grade: anosis
1 grade: the scab area that is connected accounts for less than 1% of whole leaf area;
3 grades: the scab area that is connected accounts for the 1%-10% of whole leaf area;
5 grades: the scab area that is connected accounts for the 11%-20% of whole leaf area;
7 grades: the scab area that is connected accounts for the 21%-50% of whole leaf area;
9 grades: the scab area that is connected accounts for more than 51% of whole leaf area;
Theoretical preventive effect (%)=x+y-xy
The preventive effect of the active component A that x is working concentration when being a, the preventive effect of the active component B that y is working concentration when being b.Relatively theoretical effect and actual effect, evaluates the synergy of composition, for synergistic effect when being greater than theoretical preventive effect with actual preventive effect.The results are shown in Table 2.
Table 2 contains the bactericidal composition of chitosan oligosaccharide to the control efficiency of powdery mildew of cucumber
By chitosan oligosaccharide composition, the potted plant test of powdery mildew of cucumber is found, the composition of the composition of chitosan oligosaccharide and SYP-Zo48, chitosan oligosaccharide and fenpiclonil to the effect of powdery mildew of cucumber all higher than the theoretical preventive effect of composition.Obviously, the composition of the composition of chitosan oligosaccharide and SYP-Zo48, chitosan oligosaccharide and fenpiclonil is synergy to powdery mildew of cucumber.
Embodiment 3 contains the bactericidal composition of chitosan oligosaccharide to the effect of watermelon blight
According to scheme, preparation built agent and unit dose preparation, and carry out active preliminary experiment.According to trial test, by the concentration that Formulation becomes to need.Before selecting, stubble watermelon blight morbidity field, arranges field evenly.The suspension seed-coating agent of medication different agents is evenly dressed seed (concentration is with effective milligram of gauge of every kilogram of seed with medicament), is sowed, Routine Management.Watermelon plant diseases situation is investigated when disease onset.Use Abbot formula, convert percentage to preventive effect.
Theoretical preventive effect (%)=x+y-xy
In theoretical effect, the effect of the substance A that x is concentration when being a, the effect of the substance B that y is concentration when being b, compares theoretical effect and actual effect, evaluates the synergy of composition, for synergistic effect when being greater than theoretical preventive effect with actual preventive effect.Composition the results are shown in Table 3 to watermelon disease.
Table 3 contains the bactericidal composition of chitosan oligosaccharide to the effect of watermelon blight
By containing the bactericidal composition of chitosan oligosaccharide to the test of watermelon blight, as seen from Table 3, the composition of the composition of chitosan oligosaccharide and SYP-Zo48, chitosan oligosaccharide and fenpiclonil to the effect of watermelon blight all higher than the theoretical preventive effect of correspondent composition.Obviously, the composition of SYP-Zo48 and chitosan oligosaccharide is synergy to watermelon blight.
Embodiment 4 contains the bactericidal composition of chitosan oligosaccharide to the effect of rice disease
According to scheme, preparation built agent and unit dose preparation, and carry out active preliminary experiment.According to determination of activity trial test, by the concentration that Formulation becomes to need.Select once to fall ill rice field, the wet seed dressing of medicament carries out paddy rice seed dressing (concentration is with effective milligram of gauge of every kilogram of seed with medicament), sowing, seedling.Institute an inquiry when blank bakanae disease of rice, banded sclerotial blight evil occur, often process is by 5 samplings, often 50 strains, measures the percentage that plant blade face is infected.Use Abbot formula, convert percentage to preventive effect.
Theoretical preventive effect (%)=x+y-xy
In theoretical effect, the effect of the substance A that x is concentration when being a, the effect of the substance B that y is concentration when being b, compares theoretical effect and actual effect, evaluates the synergy of composition.Composition the results are shown in Table 4 to rice plant disease.
Table 4 contains the bactericidal composition of chitosan oligosaccharide to the field efficacy of rice disease
As seen from Table 4, the composition of the composition of chitosan oligosaccharide and SYP-Zo48, chitosan oligosaccharide and fenpiclonil to the actual effect of bakanae disease of rice, rice sheath blight disease all higher than the theoretical preventive effect of composition.Obviously, be synergy for the composition of examination chitosan oligosaccharide and the composition of SYP-Zo48, chitosan oligosaccharide and fenpiclonil to bakanae disease of rice, rice sheath blight disease.
Embodiment 5 contains the bactericidal composition of chitosan oligosaccharide to wheat powdery mildew field trial
According to scheme, preparation built agent and unit dose preparation, and carry out active preliminary experiment.According to determination of activity trial test, by the concentration that Formulation becomes to need.The wetting powder of medicament carries out dress seed (concentration is with effective milligram of gauge of every kilogram of seed with medicament), sowing, Routine Management.Institute an inquiry when blank wheat powdery mildew occurs, often process is by 5 samplings, often 50 strains, measures the percentage that plant blade face is infected.Use Abbot formula, convert percentage to preventive effect.
Theoretical preventive effect (%)=x+y-xy
The preventive effect of the active component A that x is working concentration when being a, the preventive effect of the active component B that y is working concentration when being b.Relatively theoretical effect and actual effect, evaluates the synergy of composition, for synergistic effect when being greater than theoretical preventive effect with actual preventive effect.The control of bactericidal composition containing chitosan oligosaccharide to wheat powdery mildew the results are shown in Table 5.
Table 5 contains the bactericidal composition of chitosan oligosaccharide to the field efficacy of wheat powdery mildew
By the bactericidal composition containing chitosan oligosaccharide, wheat powdery mildew test is found, the composition of the composition of chitosan oligosaccharide and SYP-Zo48 and chitosan oligosaccharide and fenpiclonil to the effect of wheat powdery mildew all higher than the theoretical preventive effect of correspondent composition.Obviously, the composition of chitosan oligosaccharide and SYP-Zo48 and the composition of chitosan oligosaccharide and fenpiclonil are synergy to wheat powdery mildew.
Embodiment 6 contains the bactericidal composition of chitosan oligosaccharide to sclerotinia rot of colza field trial
According to potted plant mensuration trial test, before selecting, the Rapeseed Field in stubble morbidity field, carries out dress seed (concentration is with effective milligram of gauge of every kilogram of seed with medicament) with the seed dressing of preparation, sowing, Routine Management.Institute an inquiry when blank sclerotinia rot of colza occurs, often process is by the occurring degree of 10 investigation stems, often 5 strains.Use Abbot formula, convert percentage to preventive effect.
Theoretical preventive effect (%)=x+y-xy
The preventive effect of the active component A that x is working concentration when being a, the preventive effect of the active component B that y is working concentration when being b.Relatively theoretical effect and actual effect, evaluates the synergy of composition, for synergistic effect when being greater than theoretical preventive effect with actual preventive effect.The results are shown in Table 6.
Table 6 contains the bactericidal composition of chitosan oligosaccharide to the control efficiency of sclerotinia rot of colza
By containing the bactericidal composition of chitosan oligosaccharide to the test of sclerotinia rot of colza, as seen from Table 6, the composition of the composition of chitosan oligosaccharide and SYP-Zo48 and chitosan oligosaccharide and fenpiclonil to the actual preventive effect of sclerotinia rot of colza all higher than the theoretical preventive effect of correspondent composition.Obviously, the composition of chitosan oligosaccharide and SYP-Zo48 and the composition of chitosan oligosaccharide and fenpiclonil are synergy to sclerotinia rot of colza.
Claims (6)
1. the bactericidal composition containing chitosan oligosaccharide, it is characterized in that said composition contains A, B two kinds of components, component A is chitosan oligosaccharide, and B component is SYP-Zo48 or fenpiclonil, chitosan oligosaccharide and SYP-Zo48 ratio of quality and the number of copies are 1:12-20:1, and chitosan oligosaccharide and fenpiclonil ratio of quality and the number of copies are 1:16-20:1.
2. bactericidal composition according to claim 1, the quality sum of two components accounts for the 1-90% of bactericidal composition gross mass percentage.
3. bactericidal composition according to claim 2, said composition is made into suspending agent, suspending agent seed coat agent, wet seed dressing, wetting powder, water dispersible granules, microemulsion, for dressing seed, filling with root, spray, mixing soil or spread fertilizer over the fields.
4. bactericidal composition according to claim 3 is for preventing and treating the purposes of crop pest, disease has the fungus-caused gray mold of Fungi Imperfecti, banded sclerotial blight, fusarium wilt, the fungus-caused powdery mildew of Ascomycotina, bakanae disease, stalk break, the banded sclerotial blight that Basidiomycotina causes, rust, smut, the fungus-caused downy mildew of Mastigomycotina, late blight.
5. purposes according to claim 4, the controlling disease of composition has gray mold, powdery mildew, fusarium wilt, bakanae disease, banded sclerotial blight, stalk break.
6. the purposes according to claim 4 or 5, crop is paddy rice, wheat, cucumber, watermelon, apple, grape, oranges and tangerines, pears, lichee or flowers.
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| CN104170833B (en) * | 2014-09-02 | 2016-01-13 | 海南正业中农高科股份有限公司 | Containing the composition of oligosaccharides and poly hexamethylene biguanide or its salt |
| CN107668194A (en) * | 2017-10-18 | 2018-02-09 | 山东省果树研究所 | Fludioxonil combines application of the chitosan oligosaccharide in preventing and treating fruit, vegetable storage disease |
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| TWI421030B (en) * | 2006-12-12 | 2014-01-01 | Bayer Cropscience Ag | Pesticidal composition comprising a synthetic compound useful as nodulation agent of leguminous plants and a fungicide compound |
| CN101816305A (en) * | 2010-05-09 | 2010-09-01 | 海南正业中农高科股份有限公司 | Bactericide pesticide composite containing oligochitosan |
| CA2823999C (en) * | 2011-03-10 | 2020-03-24 | Bayer Intellectual Property Gmbh | Use of lipochito-oligosaccharide compounds for safeguarding seed safety of treated seeds |
| CN103493828B (en) * | 2013-09-11 | 2015-08-12 | 江苏耘农化工有限公司 | Microbicide compositions containing chitosan oligomer |
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