CN103493828B - Microbicide compositions containing chitosan oligomer - Google Patents
Microbicide compositions containing chitosan oligomer Download PDFInfo
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Abstract
Microbicide compositions containing chitosan oligomer, include chitosan oligomer and the second active component, it is characterized in that: the mass ratio of described chitosan oligomer and the second active component is 1:100 ~ 100:1, described second active component is the one in pyrimorph, kresoxim-methyl, Fluoxastrobin, Difenoconazole, phonetic mould amine or dimethomorph.All the other are allow in agricultural chemicals to use and acceptable auxiliary agent.Chitosan oligomer and second active component of the present composition are reasonably combined by a certain percentage, and mixture becomes wetting powder, water dispersible granules, suspending agent.The present composition, has notable synergistic effect, and stimulates crop production, has high yield effect, is mainly used in the disease of preventing and treating fruit tree, vegetables, cereal crop, solves single dose use and easily produces resistance problem.
Description
Technical field
The present invention relates to the pesticide bacteriostat composition of a kind of chitosan-containing (chitosan oligomer).
Background technology
Chemical control is the main effective means of preventing and treating corps diseases in agricultural production.But the pathogen of numerous species creates resistance to the agricultural chemicals that some frequently use in recent years, the resistance problems as the phytopathogen such as Powdery Mildew, anthrax bacteria is very general, becomes a great problem of chemical control.Because germ produces resistance, cause frequent dispenser and cause peasant burden to increase the weight of and increasing environmental pollution.In some disease, serious region occurs, conventional pesticide is used alone and is difficult to control, and poor effect.Therefore, efficient, safety, the new type bactericide of environmental protection and practicable prevention and treatment method is badly in need of finding.
Chitin (Chitin) is that natural world uniquely contains amino homogeneous state polysaccharide, and formal name used at school is [(Isosorbide-5-Nitrae)-2-acetylaminohydroxyphenylarsonic acid 2-deoxidation-β-D-Glucose], and molecular formula is (C
8h
13nO
5) n.Shitosan (Chitosan) is the most important derivative of chitin, it is the product that chitin deacetylase degree reaches more than 70%, it is the organic nitrogen source that beyond isolating protein, nitrogen content is maximum, also be the unique alkaline polysaccharide of occurring in nature, its molecular weight is usually extremely up to a million at hundreds of thousands, mainly be present on the shell of the invertebrate such as insects and aquatic shell-fish, and the cell wall of Mycophyta.Due to the generation of the deacetylated rear free amine group of chitin, shitosan is made to be easy to be dissolved in most of organic acid and inorganic acid, and insoluble in water.If but take felicity condition, it is degraded, when its mean molecule quantity is less than 10,000, then becomes low-molecular water-soluble chitosan and be called for short chitosan oligomer (Chitooligosaccharides).The oligosaccharide that degradation of chitosan and 10 Glucosamines obtained are formed by connecting with β-Isosorbide-5-Nitrae-glycosidic bond, be called chitosan oligosaccharide, be also Chitosan poly oligosaccharide or chitin oligo saccharide, formal name used at school is β-Isosorbide-5-Nitrae-oligosaccharides-gucosamine.Chitosan oligosaccharide or chitooligosaccharide-are the high-grade products of chitin, shitosan series of products, possess the outstanding features such as good water solubility, biologically active is high, function greatly, is easily absorbed by the body, have act on widely at agriculture field.
Agriculturally, utilize shitosan for raw material, adopt modern biotechnology to decompose, extract and add the fertilizer that various trace elements (plant growth institute required) refines, because of its agriculturally acquired by good result, be described as " plant vaccine " by people.Shitosan, in agricultural production application, can not only make crop growing state good, robust plant, and photosynthesis strengthens, and also shows positive effect in resistance, disease and insect resistance, stablizes and significant the effect of increasing income so have.In addition, shitosan also improves the improvement of crop quality and food value and economic worth larger meaning.
On the other hand, shitosan can be used as plant growth regulator, promotes that crop yield improves, and also can be used as soil conditioner and for gathering in the crops the fresh-keeping etc. of rear agricultural product.Especially it is suppressing the focus becoming people's research in plant disease gradually.Large quantity research shows, shitosan can inducing plant produce wide spectrum resistance, to pathogenic because of spore germination and mycelial growth all have inhibition, and have inducing action, the self-defence ability of enhancing plant, the growth of suppression various plants pathogenic microorganism with to plant to the protection function that cause of disease mattress infects, and it is nontoxic and can be microbial degradation, pollution can not be produced to environment.This makes it likely play an important role in agricultural production as a kind of novel environment friendly agricultural.
Summary of the invention
The invention provides a kind of efficient, environmental protection and be conducive to the bactericidal composition of germ resistance management, the bactericidal action of chitosan oligomer can be made full use of, the bactericidal effect of another kind of pesticide activity component is impelled greatly to improve, and delay the drug-fast generation speed of germ, can plant growth be promoted simultaneously, improve quality of agricultural product.This bactericidal composition is mainly used in the disease of preventing and treating the crops such as Cereal, fruit tree, vegetables.
The technical scheme completing foregoing invention task is as follows:
A kind of microbicide compositions containing chitosan oligomer, include chitosan oligomer and the second active component, it is characterized in that: the mass ratio of described chitosan oligomer and the second active component is 1:100 ~ 100:1, described second active component is the one in pyrimorph, kresoxim-methyl, Fluoxastrobin, Difenoconazole, phonetic mould amine or dimethomorph.
Further improvement, the mass ratio of chitosan oligomer described in the present invention and the second active component is 1:50 ~ 50:1;
The optimum quality ratio of described chitosan oligomer and the second active component is 1:20 ~ 20:1.
Second active component of the present invention is selected from the one in following compound:
1) pyrimorph (Pyrimorph), it is a kind of novel propylene acid amide fungicides, its mechanism of action has strong inhibitory action to the respiratory chain of pathogen, and being better than dimethomorph, ubiquinone-cytochrome reductase (Complex II I) is one of action target of this series bactericidal agent.
2) kresoxim-methyl (kresoxim-methyl), is absorption wide spectrum low toxicity bactericide in a kind of methoxy acrylic, has protection, treats, roots out, permeates, interior suction and slowly mobile active to top, belongs to mitochondrial respiratory inhibitor.Its sterilization mechanism suppresses mitochondrial breathing by T suppression cell pigment Bcl to electro transfer between Cl, and the energy destroying germ is formed, and finally causes germ dead.
3) Fluoxastrobin (Azoxystrobin), it is a class methoxy acrylic bactericide, efficiently, wide spectrum, all have good activity to nearly all mycota (Ascomycotina, Basidiomycotina, Mastigomycotina and Deuteromycotina) disease such as powdery mildew, rust, glume blight, net blotch, downy mildew, rice blast etc.By the energy synthesis suppressing the respiration of germ to destroy germ.
4) Difenoconazole (Difenoconazole) is a kind of triazole bactericidal agent, interior absorption sterilization, tool protection and therapeutic action.Its mechanism of action disturbs the normal growth of pathogen by suppressing the biosynthesis of ergosterol, the inhibitory action strong to the sporulation of phytopathogen, and in controlling disease process, show prophylactic treatment and root out three large effects.
5) phonetic mould amine (Pyrimethanil), also known as methylpyrimidine amine, diformazan pyrilamine, belongs to pyrimidine fungicides bactericide, has special efficacy to gray mold.Its bactericidal action mechanism is unique, by suppressing the secretion of infection process enzyme thus stoping infection process, and kills germ.There is protection and therapeutic action, there is interior suction and fumigation action simultaneously.
6) dimethomorph (Dimethomorph), also known as frost peace, An Ke, special gram, hero gram, generation is weeded, be a kind of acrylamide germicide, interior suction therapeutic, kills Oomycete fungal, its mechanism of action is the formation destroying germ cell envelope, causes the decomposition of spore cyst wall, and makes germ dead.
The present composition increases auxiliary element (auxiliary agent) according to the method that those skilled in the art of the present technique are known, and the formulation that can prepare is wetting powder, water dispersible granules, suspending agent.
In above various formulation, the mass ratio of chitosan oligomer and the second active component summation is 5%-80%.
Described auxiliary element (auxiliary agent) is solvent, filler, wetting agent, dispersant, stabilizing agent, antifreezing agent, preservative, disintegrant be or/and one or more in thickener.
For wetting powder, the additional proportion that those skilled in the art are familiar with the corresponding auxiliary agent of use and routine thereof completes the present invention very much.Dispersant is as one or more in polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate; Wetting agent is as one or more in alkyl sulfate, alkylsulfonate, naphthalene sulfonate; Filler is as one or more in ammonium sulfate, urea, sucrose, glucose, diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, potter's clay.
For water dispersible granules, operable dispersant as polycarboxylate, one or more in lignosulfonates, alkylnaphthalene sulfonate; Wetting agent is as one or more in alkyl sulfate, alkylsulfonate, naphthalene sulfonate; Disintegrant as in ammonium sulfate, urea, sucrose, glucose one or more; Binding agent is as one or more in diatomite, corn starch, PVA, carboxymethyl (ethyl cellulose); Filler soil is as one or more in diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, potter's clay.
For suspending agent, operable auxiliary agent has: dispersant is as one or more in polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate; Emulsifier is as Nongru-700 #(common name alkyl phenol methyl urea formaldehyde polyvinylether), TERSPERSE4894, TERSPERSE2500, agriculture breast 2201#, this dish-60#(common name sorbitan monostearate), agriculture breast 1601#(common name triphenoethyl benzene phenol polyoxyethylene polyoxypropylene block polymer), soprohor FD, soprohorsc, TF-03, TERSPERSE4894(2500) in one or more; Wetting agent is as one or more in alkylphenol-polyethenoxy base ether formaldehyde condensation products phosphate, alkylphenol-polyethenoxy base ether phosphate, phenethyl phenol polyethenoxy base ether phosphate, alkyl sulfate, naphthalene sulfonate; Thickener is as one or more in xanthans, polyvinyl alcohol, bentonite, aluminium-magnesium silicate; Preservative is as one or more in formaldehyde, benzoic acid, Sodium Benzoate; Defoamer is as organic silicon defoamer; Antifreezing agent is if ethylene glycol, propane diols, glycerine, urea, inorganic salts are as one or more in sodium chloride.The auxiliary agent used adopts conventional additional proportion.
Inventor found through experiments, what the present invention proposed has obvious synergistic effect containing the composite of bactericide such as chitosan oligomer and pyrimorph, kresoxim-methyl, Fluoxastrobin, Difenoconazole, phonetic mould amine, dimethomorph, significantly improves the control efficiency of corps diseases.Chitosan oligomer can produce the resistance of wide spectrum by inducing plant, strengthen the self-defence ability of plant, suppress the growth of various plants pathogenic microorganism, fungicidal spectrum can be expanded with bactericide compounded, solve the resistance generation being used alone bactericide and may causing, degradation problem under drug effect, and fungicidal activity composition usage amount can be reduced, reduce residue of pesticide and the pollution to environment, improve the quality of agricultural product simultaneously.Compared with prior art, composition of the present invention has following advantage: after one, adding chitosan oligomer, bactericide synergetic synergy is remarkable, active high, effective; Two: expand control spectrum; Three, decrease dosage, reduce cost and alleviate the pollution to environment; Four, have the effect of protection and treatment concurrently, the output of crop can be improved while controlling disease.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, the following specific embodiment of the present invention is described, but the present invention is limited to absolutely not these examples.The following stated is only the good embodiment of the present invention, only in order to explain the present invention, therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
The formulation that composition pesticide of the present invention can be prepared has wetting powder, water dispersible granules and suspending agent.Percentage in all formulas is all weight percentage.The processing technology of the various preparation of the present composition is prior art, can change to some extent according to different situations.
Embodiment 1:30% pyrimorph water dispersible granules,
Take pyrimorph 30%, chitosan oligomer 1%, pyrrolidone polymer 5%, alkylphenol polyoxyethylene 7.2%, naphthalene sulfonic acid formaldehyde polymer 6%, defoamer 1%, ethyl cellulose 5%, kaolin complements to 100%.By above-mentioned raw materials through routine mixing, ultra micro air-flow crushing, make water dispersible granules through certain pore size screen cloth extruder grain, oven dry, screening.
Embodiment 2:25% azoxystrobin water dispersible granule,
Take Fluoxastrobin 25%, chitosan oligomer 1%, pyrrolidone polymer 5%, alkylphenol polyoxyethylene 7.2%, naphthalene sulfonic acid formaldehyde polymer 6%, defoamer 1%, ethyl cellulose 5%, kaolin complements to 100%.By above-mentioned raw materials through routine mixing, ultra micro air-flow crushing, make water dispersible granules through certain pore size screen cloth extruder grain, oven dry, screening.
Embodiment 3:50% water dispersion granules agent of Kresoxim,
Take kresoxim-methyl 50%, chitosan oligomer 2%, naphthalene sulfonic acid formaldehyde polymer 10%, alkylphenol polyoxyethylene 8.2%, adhesive C MC2%, pyrrolidone polymer 5%, defoamer 1%, kaolin complements to 100%.By above-mentioned raw materials through routine mixing, ultra micro air-flow crushing, make water dispersible granules through certain pore size screen cloth extruder grain, oven dry, screening.
Embodiment 4:, 40% dimethomorph wetting powder,
Take dimethomorph 40%, chitosan oligomer 2%, calcium lignosulfonate 6%, alkyl naphthalene sulfonic acid formaldehyde condensation products 4%, lauryl sodium sulfate 1.5%, white carbon 10%, kaolin complements to 100%.By above-mentioned raw materials pulverizing, ultra micro air-flow crushing, the obtained 40% dimethomorph wetting powder of mixing.
Embodiment 5:20% pyrimorph wetting powder,
Take pyrimorph 20%, chitosan oligomer 1%, calcium lignosulfonate 4%, alkyl naphthalene sulfonic acid formaldehyde condensation products 2%, lauryl sodium sulfate 0.8%, white carbon 8%, kaolin complements to 100%.By above-mentioned raw materials pulverizing, ultra micro air-flow crushing, the obtained 20% pyrimorph wetting powder of mixing.
Embodiment 6:12.5% pyrimorph suspending agent,
Take pyrimorph 12.5%, chitosan oligomer 1%, TERSPERSE48944%, TERSPERSE25002%, xanthans 0.3%, propane diols 5%, benzoic acid 0.5%, defoamer 1%, deionized water complements to 100%.Above-mentioned raw materials, through mixing, adds high-speed shearing device and shears 30 minutes, with the obtained 12.5% pyrimorph suspending agent of sand mill sand milling.
The agent of embodiment 7:20% difenoconazole suspending,
Take Difenoconazole 20%, chitosan oligomer 2%, TERSPERSE489410%, TERSPERSE25005%, xanthans 0.5%, propane diols 10%, benzoic acid 0.5%, defoamer 1%, deionized water complements to 100%.Above-mentioned raw materials, through mixing, adds high-speed shearing device and shears 30 minutes, with the obtained 20% difenoconazole suspending agent of sand mill sand milling.
Embodiment 8:40% Fluoxastrobin suspending agent,
Take Fluoxastrobin 40%, chitosan oligomer 4%, TERSPERSE489414%, TERSPERSE250010%, xanthans 1%, propane diols 10%, benzoic acid 0.5%, defoamer 2%, deionized water complements to 100%.Above-mentioned raw materials, through mixing, adds high-speed shearing device and shears 30 minutes, with the obtained 40% Fluoxastrobin suspending agent of sand mill sand milling.
Embodiment 9, substantially the same manner as Example 1, but change 30% pyrimorph water dispersible granules into, component changes into: take phonetic mould amine 30%, chitosan oligomer 1%.The wherein mass ratio of chitosan oligomer and the second active component summation is 50%.
Embodiment 10, substantially the same manner as Example 1, but the mass ratio that the mass ratio of component is chitosan oligomer and pyrimorph is 1:100.The wherein mass ratio of chitosan oligomer and the second active component summation is 5%.
Embodiment 11, substantially the same manner as Example 1, but the mass ratio that the mass ratio of component is chitosan oligomer and pyrimorph is 100:1.The wherein mass ratio of chitosan oligomer and the second active component summation is 80%.
Embodiment 12, substantially the same manner as Example 1, but the mass ratio that the mass ratio of component is chitosan oligomer and pyrimorph is 50:50.
Embodiment 13, substantially the same manner as Example 1, but the mass ratio that the mass ratio of component is chitosan oligomer and pyrimorph is 20:80.
Embodiment 14, substantially the same manner as Example 2, but the mass ratio that the mass ratio of component is chitosan oligomer and Fluoxastrobin is 1:100.
Embodiment 15, substantially the same manner as Example 2, but the mass ratio that the mass ratio of component is chitosan oligomer and Fluoxastrobin is 100:1.
Embodiment 16, substantially the same manner as Example 2, but the mass ratio that the mass ratio of component is chitosan oligomer and Fluoxastrobin is 50:50.
Embodiment 17, substantially the same manner as Example 2, but the mass ratio that the mass ratio of component is chitosan oligomer and Fluoxastrobin is 20:80.
Embodiment 18, substantially the same manner as Example 2, but the mass ratio that the mass ratio that the mass ratio of component is chitosan oligomer and Fluoxastrobin is described chitosan oligomer and the second active component is 1:50.
Embodiment 19, substantially the same manner as Example 2, but the mass ratio that the mass ratio that the mass ratio of component is chitosan oligomer and Fluoxastrobin is described chitosan oligomer and the second active component is 50:1.
Embodiment 20, substantially the same manner as Example 2, but the mass ratio that the mass ratio of component is chitosan oligomer and Fluoxastrobin is 1:20.
Embodiment 21, substantially the same manner as Example 2, but the mass ratio that the mass ratio of component is chitosan oligomer and Fluoxastrobin is 20:1.
Embodiment 22, substantially the same manner as Example 3, but the mass ratio that the mass ratio of component is chitosan oligomer and kresoxim-methyl is 1:100.
Embodiment 23, substantially the same manner as Example 3, but the mass ratio that the mass ratio of component is chitosan oligomer and kresoxim-methyl is 100:1.
Embodiment 24, substantially the same manner as Example 3, but the mass ratio that the mass ratio of component is chitosan oligomer and kresoxim-methyl is 50:50.
Embodiment 25, substantially the same manner as Example 3, but the mass ratio that the mass ratio of component is chitosan oligomer and kresoxim-methyl is 20:80.
Embodiment 26, substantially the same manner as Example 3, but the mass ratio that the mass ratio of component is chitosan oligomer and kresoxim-methyl is 1:50.
Embodiment 27, substantially the same manner as Example 3, but the mass ratio that the mass ratio of component is chitosan oligomer and kresoxim-methyl is 50:1.
Embodiment 28, substantially the same manner as Example 3, but the mass ratio that the mass ratio of component is chitosan oligomer and kresoxim-methyl is 1:20.
Embodiment 29, substantially the same manner as Example 3, but the mass ratio that the mass ratio of component is chitosan oligomer and kresoxim-methyl is 20:1.
Embodiment 30, substantially the same manner as Example 4, but the mass ratio that the mass ratio of component is chitosan oligomer and dimethomorph is 1:100.
Embodiment 31, substantially the same manner as Example 4, but the mass ratio that the mass ratio of component is chitosan oligomer and dimethomorph is 100:1.
Embodiment 32, substantially the same manner as Example 4, but the mass ratio that the mass ratio of component is chitosan oligomer and dimethomorph is 50:50.
Embodiment 33, substantially the same manner as Example 4, but the mass ratio that the mass ratio of component is chitosan oligomer and dimethomorph is 20:80.
Embodiment 34, substantially the same manner as Example 4, but the mass ratio that the mass ratio of component is chitosan oligomer and dimethomorph is 1:50.
Embodiment 35, substantially the same manner as Example 4, but the mass ratio that the mass ratio of component is chitosan oligomer and dimethomorph is 50:1.
Embodiment 36, substantially the same manner as Example 4, but the mass ratio that the mass ratio of component is chitosan oligomer and dimethomorph is 1:20.
Embodiment 37, substantially the same manner as Example 4, but the mass ratio that the mass ratio of component is chitosan oligomer and dimethomorph is 20:1.
Embodiment 38, substantially the same manner as Example 7, but the mass ratio that the mass ratio of component is chitosan oligomer and Difenoconazole is 1:100.
Embodiment 39, substantially the same manner as Example 7, but the mass ratio that the mass ratio of component is chitosan oligomer and Difenoconazole is 100:1.
Embodiment 40, substantially the same manner as Example 7, but the mass ratio that the mass ratio of component is chitosan oligomer and Difenoconazole is 50:50.
Embodiment 41, substantially the same manner as Example 7, but the mass ratio that the mass ratio of component is chitosan oligomer and Difenoconazole is 20:80.
Embodiment 42, substantially the same manner as Example 8, but the mass ratio that the mass ratio of component is chitosan oligomer and Difenoconazole is 1:100.
Embodiment 43, substantially the same manner as Example 8, but the mass ratio that the mass ratio of component is chitosan oligomer and Difenoconazole is 100:1.
Embodiment 44, substantially the same manner as Example 8, but the mass ratio that the mass ratio of component is chitosan oligomer and Difenoconazole is 50:50.
Embodiment 45, substantially the same manner as Example 8, but the mass ratio that the mass ratio of component is chitosan oligomer and Difenoconazole is 20:80.
Embodiment 46, substantially the same manner as Example 8, but the mass ratio that the mass ratio of component is chitosan oligomer and Difenoconazole is 1:50.
Embodiment 47, substantially the same manner as Example 8, but the mass ratio that the mass ratio of component is chitosan oligomer and Difenoconazole is 50:1.
Embodiment 48, substantially the same manner as Example 8, but the mass ratio that the mass ratio of component is chitosan oligomer and Difenoconazole is 1:20.
Embodiment 49, substantially the same manner as Example 8, but the mass ratio that the mass ratio of component is chitosan oligomer and Difenoconazole is 20:1.
Embodiment 50, substantially the same manner as Example 9, but the mass ratio that the mass ratio of component is chitosan oligomer and phonetic mould amine is 1:100.
Embodiment 51, substantially the same manner as Example 9, but the mass ratio that the mass ratio of component is chitosan oligomer and phonetic mould amine is 100:1.
Embodiment 52, substantially the same manner as Example 9, but the mass ratio that the mass ratio of component is chitosan oligomer and phonetic mould amine is 50:50.
Embodiment 53, substantially the same manner as Example 9, but the mass ratio that the mass ratio of component is chitosan oligomer and phonetic mould amine is 20:80.
Embodiment 54, substantially the same manner as Example 9, but the mass ratio that the mass ratio of component is chitosan oligomer and phonetic mould amine is 1:50.
Embodiment 55, substantially the same manner as Example 9, but the mass ratio that the mass ratio of component is chitosan oligomer and phonetic mould amine is 50:1.
Embodiment 56, substantially the same manner as Example 9, but the mass ratio that the mass ratio of component is chitosan oligomer and phonetic mould amine is 1:20.
Embodiment 57, substantially the same manner as Example 9, but the mass ratio that the mass ratio of component is chitosan oligomer and phonetic mould amine is 20:1.
One of biologically active: toxicity measures.
Toxicity Determination to bacterium of downy mildew of cucumber:
Experimental subjects: the bacterium of downy mildew of cucumber picking up from field.
Test method: with reference to " Chinese name republic agricultural industry criteria NY/T1154.7-2006 ".The cucumber leaves carrying Pseudoperonospora cubensis moisturizing in culture dish of picking up from field is cultivated, then with spore under aseptic washing, be mixed with the spore suspension of 5*105, and spray inoculation is carried out to cucumber seedling, carry out chemicals treatment after 24h after 24h, test medicine chooses two consistent leaf phase cucumber seedlings of growth potential see table 2., and each process selects 3 basins for examination cucumber seedling, spray under 50PSI pressure with Potter spray tower, the about 5ml of every basin.Each medicament arranges 5 concentration gradients.With spray equivalent clear water for blank.After spraying, cucumber seedling is put into artificial climate incubator to cultivate.After 7d, according to the morbidity grade scale investigation disease index of cucumber cream disease, and calculate control efficiency, then calculate concentration EC50 in suppression by method of least squares, then calculate co-toxicity coefficient (CTC) according to the abundant method of Sun Yun.Virulence test result is in table 1.
Biological activity determination two:: prevent and treat downy mildew of garpe field control effectiveness test.
Subjects is downy mildew (Plasmopara viticola), and trial crops is kyoto grape, and experiment place is the fourth village, Jurong wine-growing cooperative society, experimental field rich water is sufficient, and the grape age of tree is 7 years, and line-spacing is 1.5m × 1m, employing trellis type is cultivated, and grows fine during test.Test adopts the group arrangement of random district, every community 10 strain grape.Test process is watered dilution by 2000,3000,4000 times, adopts sharp agriculture board sprayer even spraying.Dispenser 3 times, spraying volume is 0.5 liter/strain.Test is investigated 2 times altogether, respectively investigation 1 time in 8 days after 7 days and last dispenser after first time dispenser respectively.According to agricultural chemicals field trial criterion (two), every community random searching 10 then pumping is newly climing, from top to bottom 10 blades, calculates the sick number of sheets at different levels respectively, calculates disease index and control efficiency.
Downy mildew of garpe grade scale (in units of blade) is: 0 grade (without scab); 1 grade (lesion area accounts for less than 5% of whole leaf area); 3 grades (lesion area accounts for 6% ~ 25% of whole leaf area); 5 grades (lesion area accounts for 26% ~ 50% of whole leaf area); 7 grades (lesion area accounts for 51% ~ 75% of whole leaf area); 9 grades (lesion area accounts for more than 76% of whole leaf area).
Disease index computational methods:
Disease index=(∑ (the various sick number of sheets × relative level numerical value)/investigate the total number of sheets × 9) × 100;
Preventive effect computational methods:
Preventive effect=(blank disease index-chemicals treatment disease index)/blank disease index × 100;
Result shows, has good control effects in the dispenser of downy mildew of garpe initial stage of origination to it, and after last dispenser, 8 days preventive effects all reach more than 70%, and to Growth of Grape safety, blade turns green, has certain stimulation plant strain growth effect.Concrete field control effectiveness test the results are shown in Table 2.
Claims (3)
1. the microbicide compositions containing chitosan oligomer, includes chitosan oligomer and the second active component, it is characterized in that: described second active component is pyrimorph;
The described formulation containing chitosan oligomer microbicide compositions is wetting powder, water dispersible granules or suspending agent;
In described wetting powder, also containing wetting agent, dispersant, filler;
In described water dispersible granules, also contain: dispersant, wetting agent, disintegrant, binding agent and filler soil;
In described suspending agent, also contain: dispersant, emulsifier, wetting agent, thickener, preservative, defoamer are or/and antifreezing agent;
The mass ratio of described chitosan oligomer and the second active component is 1:12.5 ~ 1:30.
2. the microbicide compositions containing chitosan oligomer according to claim 1, is characterized in that: that in the formulation of described wetting powder, water dispersible granules or suspending agent the mass ratio of chitosan oligomer and the second active component summation is 5% ~ 80%.
3. the microbicide compositions containing chitosan oligomer according to claim 1 and 2, is characterized in that:
Described dispersant is selected from: one or more in polycarboxylate, lignosulfonates, soprohor FD or alkylnaphthalene sulfonate;
Described wetting agent is selected from: one or more in alkylphenol-polyethenoxy base ether formaldehyde condensation products phosphate, soprohor sc, TF-03, alkylphenol-polyethenoxy base ether phosphate or phenethyl phenol polyethenoxy base ether phosphate;
Described disintegrant is selected from: in ammonium sulfate, urea, sucrose or glucose one or more;
Described binding agent is selected from: one or more in diatomite, corn starch, K12, PVA or carboxymethyl;
Described emulsifier is selected from: alkyl phenol methyl urea formaldehyde polyvinylether, TERSPERSE4894, TERSPERSE2500, agriculture breast 2201#, sorbitan monostearate, triphenoethyl benzene phenol polyoxyethylene polyoxypropylene block polymer or TERSPERSE4894 in one or more;
Described filler soil is selected from: one or more in diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, potter's clay;
Described preservative is selected from: one or more in formaldehyde, benzoic acid, Sodium Benzoate;
Described defoamer is selected from: silicone based defoamer;
Described antifreezing agent is selected from: one or more in ethylene glycol, propane diols, glycerine or urea.
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| CN103931626B (en) * | 2014-04-01 | 2016-03-23 | 海南正业中农高科股份有限公司 | Containing the bactericidal composition of chitosan oligosaccharide |
| CN104255739A (en) * | 2014-09-22 | 2015-01-07 | 江苏省绿盾植保农药实验有限公司 | Bactericide composition for preventing and treating wheat scab and application thereof |
| CN104381287B (en) * | 2014-11-21 | 2016-06-29 | 北京富力特农业科技有限责任公司 | Microbicide compositions and application thereof |
| CN115104612B (en) * | 2021-08-24 | 2023-10-27 | 汝阳自强生物科技有限公司 | Granule for treating melon wilt and preparation method and application thereof |
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| CN101816305A (en) * | 2010-05-09 | 2010-09-01 | 海南正业中农高科股份有限公司 | Bactericide pesticide composite containing oligochitosan |
| CN101836633A (en) * | 2010-05-14 | 2010-09-22 | 深圳市朗钛生物科技有限公司 | Pesticide composition containing amino-oligosaccharide and kresoxim-methyl and use method and application thereof |
| CN102617224B (en) * | 2012-03-07 | 2014-04-30 | 海南正业中农高科股份有限公司 | Plant wound protective agent containing chitosan oligosaccharide |
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