CN105104382A - Biological compound sterilizing composition and preparation and application thereof - Google Patents
Biological compound sterilizing composition and preparation and application thereof Download PDFInfo
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- CN105104382A CN105104382A CN201510629021.4A CN201510629021A CN105104382A CN 105104382 A CN105104382 A CN 105104382A CN 201510629021 A CN201510629021 A CN 201510629021A CN 105104382 A CN105104382 A CN 105104382A
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- bactericide
- chitosan
- kasugarnycin
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Abstract
The invention discloses a biological compound sterilizing composition and application thereof and belongs to the field of pesticide compositions. The biological compound sterilizing composition has effective active ingredients, chitosan and kasugamycin; the composition is made into wettable powder that can be a compound bactericide, which has outstanding bactericidal and preventive effects for common crop diseases, especially grape anthracnose and rice blast. Experiments show that the bactericide made with the biological compound sterilizing composition has higher evidently increase than single doses in terms of controlling effect.
Description
Technical field
The invention belongs to technical field of pesticide, relate to a kind of biological complex preparation, the bactericidal composition of especially a kind of chitosan and kasugarnycin and preventing and treating the application on bitter rot or anthracnose of grape and rice blast.
Background technology
Chitosan (English common name: chltosan) has another name called chitin, Hai Liyuan, chemical name: N-second phthalein Glucosamine polymer, molecular formula: (C
6h
11o
4n)
n.Chitosan is a kind of natural cationic polysaccharide that chitin that a class is extracted from practical insect and marine organisms obtains through deacetylation; there is degradability, well film forming and biocompatibility and the biologically active such as antibacterial, antitumor, be widely used in the industries such as medicine, food and chemical industry.In agricultural production, chitosan is a kind of excellent biogenic crop protection agents, has induction and makes the function of object resistance factor, can the pathogenic bacteria drift in film forming protection air infect and the erosion injury of pernicious gas again.Chitosan is nontoxic, is the biological regulator with fertile medicine double effects; Also be non-toxic and non-pollution thickener, the effect of thickener in preparation production can be reduced.
Kasugarnycin (English common name: kasugamycin).The metabolite that a kind of actinomycetes that Institute of Microorganism, Academia Sinica succeeds in developing produce, belongs to farm antibiotics class low toxicity bactericide; There is stronger permeability and interior absorption, and can move in plant corpus; Instant effect after spray medicine, resistance of rainwater washing against, the lasting period is long; Have preventive and therapeutic action to disease, especially result for the treatment of is more remarkable.Its bactericidal mechanism is the esterase system of interference germ metabolism of amino acid, affects protein synthesis, and suppresses mycelial growth and cause cell granulations, but do not act on spore germination.Test shows, melon spray this medicine posterior lobe look dark green, and can extend harvest time.It is to people, animal, fish avirulence, to crop without poisoning, is a kind of public nuisance-free agricultural chemicals producing pollution-free food.Kasugarnycin can be used for preventing and treating rice blast, bacterial brown streak, cladosporium leaf and fruit mould of tomato, gray mold, cucumber fusarium axysporum, bacterial angular leaf spot, peach gummosis, shot hole, the Be very effective such as shothole disease in production.
Summary of the invention
Goal of the invention: the object of the invention is to cause drug resistance issues for the single use of traditional chemical medicament, provides the biological combination sterilization composition of a kind of hypotoxicity, high preventive effect and bactericide thereof; Another object of the present invention is to provide this bactericide in the application for preventing and treating on bitter rot or anthracnose of grape and rice blast.
Technical scheme: in order to realize foregoing invention object, the invention provides a kind of novel biological combination sterilization composition, is made up of chitosan and kasugarnycin.
Traditional chemical bactericidal agent for preventing and treating bitter rot or anthracnose of grape easily develops immunity to drugs, residue of pesticide and problem of environmental pollution, and biology lures anti-chitosan single use preventive effect often undesirable, biological combination sterilization composition provided by the present invention can improve control efficiency, delays the pesticide resistance of biopesticide kasugarnycin.Utilize the bactericide that biological combination sterilization composition of the present invention is made, control efficiency is significantly better than the medicament that chitosan or kasugarnycin are made separately.
Concrete composition of the present invention, by chitosan and kasugarnycin 1:(1 ~ 20 in mass ratio) form.Preferred 1:(6 ~ 10).Research finds that, when the mass ratio of chitosan and kasugarnycin is 1:10, significantly, field efficacy is also best in synergistic effect, therefore, biological combination sterilization composition of the present invention, chitosan and kasugarnycin optimum quality ratio be 1:10.
As the active component of agricultural chemicals of the present invention, this combination sterilization composition can utilize existing conven-tional adjuvants to be made into the bactericide of various formulation, such as wetting powder, suspending agent, aqua etc., in the present invention, wetting powder is preferred dosage form, this is because: on the one hand, kasugarnycin is interior absorption medicament, and it is made wetting powder bactericidal effect and gives prominence to, on the other hand, this medicament organic solvent content is few, Environmental Safety.
Concrete, the bactericide that the present invention utilizes above-mentioned combination sterilization composition to make as active ingredient, this bactericide formulation is wetting powder, and described combination sterilization composition accounts for 5 ~ 15% of gross mass.
Further, as technical scheme of the present invention, described combination sterilization composition accounts for 6 ~ 15% of gross mass.The bactericide of the wettable powder formulation used under this proportioning ensures to obtain optimum bactericidal effect under alap active ingredient.
Present invention also offers above-mentioned bactericide in the application preventing and treating bitter rot or anthracnose of grape and rice blast, for aforesaid crop pest, bactericide contains the active ingredient of 11% by mass percentage, described active ingredient is by chitosan and the kasugarnycin composite mixing of 1:10 in mass ratio, make wetting powder, thin up sprays, and bactericide usage amount is every mu and sprays active ingredient 2.625g ~ 3g, preferred 2.75g.
Beneficial effect: a kind of biological combination sterilization composition of the present invention lures the improvement of anti-bactericide chitosan to invent based on utilization is biological, by obtaining beyond thought effect with biological antibiotic agricultural chemicals kasugarnycin is composite, to crop pest, there is significant synergy, solve chitosan and kasugarnycin to be used alone preventive effect not high simultaneously, and the resistance problems that kasugarnycin Long-Time Service causes.The Wettable powdered disinfectant of the present invention's preparation is particularly useful for the control of the important crops such as fruit tree, paddy rice disease, particularly preventing and treating bitter rot or anthracnose of grape and rice blast, the control efficiency utilizing technology of the present invention to obtain significantly is better than composite front single use, and compared with chitosan, cost declines, diseases control tool is greatly improved, and has enriched biopesticide product and the application on the control of crop disease such as grape, paddy rice.
Embodiment:
Below in conjunction with specific embodiment, the present invention is further described.
Combination sterilization composition of the present invention comprises chitosan and kasugarnycin 1:(1 ~ 20 in mass ratio) composition, preferred version is 1:(6 ~ 10), this combination sterilization composition is utilized to make bactericide agricultural chemicals, preferred formulation is wetting powder, in preparation Wettable powdered disinfectant, combination sterilization composition accounts for 5 ~ 15% of gross mass as active ingredient, and preferably 6 ~ 11%.Above-mentioned bactericide in use every mu of fountain height active ingredient is about 3g (2.625g ~ 3.2g).
Please refer to table 1, table 1 is that combination sterilization composition of the present invention is for preparing the form of five kinds of compound methods of Wettable powdered disinfectant.
The biological combination sterilization composition of table 1 is mixed with bactericide
The biological complex composition of test example 1 is to the suppression of grape anthracnose
This test example carries out indoor measurement for chitosan, kasugarnycin and different ratio thereof to the inhibitory action of grape anthracnose.Adopt mycelial growth rate method, the liquid of each single dose and built agent is diluted to desired concn successively, then 1mL liquid and 9mL medium is mixed in culture dish, make the PSA medium containing series concentration medicament, sterile water is adopted to make blank, each process repetition 4 times.The grape anthracnose of reservation is transferred in PSA plate, at 25 DEG C, activates 72h, then produce at nearly colony edge card punch the bacterium cake that diameter is 5mm, and in the PSA plate being transferred to pastille and blank.Cultivate 96h for 25 DEG C, when bacterium colony in contrasting grows to 4/5 of about plate diameter, adopt right-angled intersection method to measure colony diameter.
Calculate colony diameter average, and calculate mycelial growth average inhibition according to the following formula:
Adopt DPS13.0 professional version data handling system, calculate regression equation, EC that medicament suppresses grape anthracnose mycelial growth
50and 95% confidence limit.
According to Wadley method, calculate coefficient of synergism (SR).Evaluate the synergy type of pharmacy mix according to coefficient of synergism (SR), namely SR<0.5 is antagonism, and 0.5≤SR≤1.5 are summation action, and SR>1.5 is synergistic effect.SR＝EC
50(Eth)/EC
50(Eob)，EC
50(Eth)＝(a+b)/[(a/EC
50A)+(b/EC
50B)]。Wherein, A, B are respectively bactericide single dose, and a, b are the ratio of corresponding single dose in mixture, EC
50(Eth) be mixture EC
50theoretical value, EC
50(Eob) be mixture EC
50measured value.Gained chitosan, kasugarnycin and built agent thereof to grape anthracnose biologically active (virulence) measurement result in table 2.
Table 2 chitosan, kasugarnycin and built agent thereof are to grape anthracnose biologically active (virulence) measurement result
Chitosan of the present invention is different from kasugarnycin biological agent structure, the mechanism of action is different, and to bitter rot or anthracnose of grape without cross resistance, two kinds of composite reagents are conducive to overcoming or delay grape anthracnose to the drug-fast development of chemical agent, improve control efficiency.Please refer to shown in table 2, through indoor biological determination of activity and formulation screening test, chitosan and kasugarnycin single dose are respectively 0.0227 (0.1635-0.3184) μ g/mL and 0.2238 (0.1615-0.3113) μ g/mL to the EC50 that grape anthracnose mycelial growth suppresses, chitosan to the inhibit activities of grape anthracnose mycelial growth higher than kasugarnycin.
Chitosan and kasugarnycin are respectively with the EC that the proportioning compounded combination of 1:1,1:3,1:6,1:10 and 1:20 suppresses grape anthracnose mycelial growth
50be respectively 0.0341 (0.0242-0.0493) μ g/mL, 0.0510 (0.0357-0.0727) μ g/mL, 0.0642 (0.0464-0.0905) μ g/mL, 0.0799 (0.0574-0.1123) μ g/mL and 0.1208 (0.0843-0.1735) μ g/mL, 5 kinds of compound sterilizing combinations are 120.88,136.49,153.87,155.16 and 130.31 to the co-toxicity coefficient (CTC) of grape anthracnose.Above result shows, chitosan and kasugarnycin all show synergistic effect so that 1:1,1:3,1:6,1:10 and 1:20 proportioning is composite to grape anthracnose, and wherein 1:6 and 1:10 ratio activity is the strongest, and synergistic effect is remarkable.
The control efficiency of test example 2 bitter rot or anthracnose of grape and rice blast
This test is carried out for the control efficiency of bitter rot or anthracnose of grape and rice blast.
Peasant household of Xia Dian village one, Huayang town, bitter rot or anthracnose of grape test site Jurong vineyard, grape is summer black, the outdoor cultivation of hedge frame, the age of tree 4 years.The bactericide made by the combination sterilization composition of above-described embodiment 1, embodiment 2, embodiment 3, embodiment 4, the embodiment 5 respectively respective consumption different by 50g, 40g, 20g, 25g, 25g uses, 2% chitosan aqua every mu of 150g, and 2% kasugarnycin wetting powder every mu of 120g, clear water contrasts, totally 8 process, often process repetition 3 times, often repeat 3 strains.Every mu of water consumption 50kg.On June 9th, 2014, grape bagging sprayed once with fair weather bagging on June 14 same day for first 5 days again, and emphasis sprays fruit ear, sprays twice altogether, and second time fruit ear liquid timely bagging on the dry rear same day, often repeats equal random searching 10 fruit ears, often process 30 fruit ears.Investigate the total grain number of each process and anthrax infected seed number before gathering after August 10, grape was painted, calculate diseased seedrate, with the fruit ear grain incidence of disease of clear water check plot for contrast, calculate the average preventive effect of chemicals treatment.
Rice blast carries out at peasant household of stone lion village one, Huayang town, Jurong paddy field.Rice varieties is town rice No. 18, dry-raising and sparse-planting transplant field, the bactericide made by the combination sterilization composition of above-described embodiment 1, embodiment 2, embodiment 3, embodiment 4, the embodiment 5 respectively respective consumption different by 50g, 40g, 20g, 25g, 25g uses, 2% chitosan aqua every mu of 150g, and 2% kasugarnycin wetting powder every mu of 120g, clear water contrasts, totally 8 process, often process repetition 3 times, often repeat 10 square metres.Every mu of water consumption 50kg.Ear and respectively spray once with during heading 5% for first 10 days, 15 days investigation panicle blast incidences and disease index after second time medicine, refer to calculate preventive effect in contrast with clear water contrast disease.
Its result is as shown in table 4:
Table 4 chitosan and kasugarnycin and mixture preparation prevent and treat the field control effect of bitter rot or anthracnose of grape and rice blast
As can be seen from Table 4, bitter rot or anthracnose of grape is tested, under the composition of embodiment one to embodiment five uses 50g, 40g, 20g, 25g, 25g dosage respectively by every mu, active ingredient is 3g, 3.2g, 2.8g, 2.75g, 2.625g by actual mu consumption respectively; Single dose process 2% chitosan aqua 150ml, active ingredient is by actual mu consumption 3g, 2% kasugarnycin wetting powder 120g, active ingredient is by actual mu consumption 2.4g, can be found by above-mentioned experiment, the bactericide obtained under utilizing the preparation of chitosan, kasugarnycin 1:20 in mass ratio, mu uses effective dose about 3 grams, anthracnose diseased seedrate is all obtained to the preventive effect of more than 80%, all apparently higher than 2% chitosan aqua (preventive effect 64.53%) and 2% kasugarnycin wetting powder (preventive effect is 72.77%).Particularly 11% wetting powder that is mixed with of embodiment four (chitosan, kasugarnycin 1:10 in mass ratio), mu preparation consumption 25g, the preventive effect of acquisition is up to 88.42%, and control efficiency is significantly higher than each single dose preventive effect.
To rice blast test, under the composition of embodiment one to embodiment five uses 50g, 40g, 20g, 25g, 25g dosage respectively by every mu, active ingredient is 3g, 3.2g, 2.8g, 2.75g, 2.625g by actual mu consumption respectively; Single dose process 2% chitosan aqua 150ml, active ingredient is by actual mu consumption 3g, 2% kasugarnycin wetting powder 120g, active ingredient is by actual mu consumption 2.4g, can be found by above-mentioned experiment, the bactericide obtained under utilizing the preparation of chitosan, kasugarnycin 1:20 in mass ratio, mu uses effective dose about 3 grams, Rice blast disease is obtained to the preventive effect of 78.65%-85.32%, all apparently higher than 2% chitosan aqua (preventive effect 67.32%) and 2% kasugarnycin wetting powder (preventive effect is 74.46%).Particularly 11% wetting powder that is mixed with of embodiment four (chitosan, kasugarnycin 1:10 in mass ratio), mu preparation consumption 25g, the preventive effect of acquisition is up to 85.32%, and control efficiency is significantly higher than each single dose preventive effect.
The above is only the preferred embodiment of the present invention; be noted that for those skilled in the art; under the premise without departing from the principles of the invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (7)
1. a biological combination sterilization composition, is characterized in that said composition is made up of chitosan and kasugarnycin, and both mass ratioes are 1:(1 ~ 20).
2. biological combination sterilization composition as claimed in claim 1, is characterized in that chitosan and kasugarnycin, and both mass ratioes are 1:(6 ~ 10).
3. biological combination sterilization composition as claimed in claim 1, is characterized in that chitosan and kasugarnycin, and both mass ratioes are 1:10.
4. the bactericide that the biological combination sterilization composition in claims 1 to 3 described in any one is made, is characterized in that: this bactericide formulation is wetting powder, and described combination sterilization composition accounts for 1 ~ 15% of gross mass.
5. bactericide as claimed in claim 4, is characterized in that: described combination sterilization composition accounts for 6 ~ 15% of gross mass.
6. the application of bactericide as claimed in claim 4, is characterized in that for preventing and treating bitter rot or anthracnose of grape and rice blast.
7. apply as claimed in claim 6, it is characterized in that: this bactericide contains the active ingredient of 6 ~ 14% by mass percentage, and thin up sprays, bactericide usage amount is every mu and sprays active ingredient 2.625g ~ 3.2g.
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| CN114946861A (en) * | 2022-05-28 | 2022-08-30 | 河北上瑞生物科技有限公司 | Pesticide composition containing chitosan and kasugamycin, preparation method and application |
| WO2022185036A1 (en) * | 2021-03-01 | 2022-09-09 | UPL Corporation Limited | Agricultural composition comprising kasugamycin |
| WO2022185037A1 (en) * | 2021-03-01 | 2022-09-09 | UPL Corporation Limited | Agricultural composition comprising kasugamycin |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022185036A1 (en) * | 2021-03-01 | 2022-09-09 | UPL Corporation Limited | Agricultural composition comprising kasugamycin |
| WO2022185037A1 (en) * | 2021-03-01 | 2022-09-09 | UPL Corporation Limited | Agricultural composition comprising kasugamycin |
| CN114946861A (en) * | 2022-05-28 | 2022-08-30 | 河北上瑞生物科技有限公司 | Pesticide composition containing chitosan and kasugamycin, preparation method and application |
| CN114946861B (en) * | 2022-05-28 | 2023-10-31 | 河北上瑞生物科技有限公司 | Pesticide composition containing chitosan and kasugamycin, preparation method and application |
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Application publication date: 20151202 |