Containing composition and the application of Acibenzolar and chitosan oligosaccharide
Technical field
The invention belongs to plant protection art, particularly relate to the composition containing Acibenzolar and chitosan oligosaccharide and application.
Background technology
Raising along with people's living standard and the concern to ecotope, people improve day by day to the demand of fruit, vegetables and quality.But how to make fruit, vegetable increase production increases income becomes agricultural cultivation industry urgent problem, the application of plant growth regulator can promote the growth of plant, improves output and strengthen resistance, is one of major measure of gourd, fruit and vegetable increasing both production and income.Product of the present invention is widely used in multiclass plant cultivation, as crop, fruit tree, vegetables, flowers etc., impels plant disease-proof, promotes growing way and volume increase, and containing the development of oligosaccharide product to Development of Pollution-free Agricultural Products, Developing Sustainable Agriculture is significant.
Acibenzolar is diazosulfide carboxylic acid esters Plant activator.Acibenzolar English name is acibenzolar, and chemical name is benzo (1,2,3) thiadiazoles-7-carbothioic acid-S-methyl esters, and molecular formula is C
8h
6n
2oS
2, Acibenzolar obtains resistance by the defense response activating crop self, thus makes plant produce the self-protection effect of wide spectrum to multiple fungi, bacterium, nematode and virus.
Chitosan oligosaccharide is as the important plant inducing anti-disease agent of a class, derive from the research of the oligosaccharide of plant, research shows, chitosan oligosaccharide is in the process resisting pathogen erosion, can induction plant be excited to produce a series of defense reaction, these defense reactions synergy stops propagation and the invasion of pathogen, reaches the effect of preventing and curing diseases.It is reported, the derivative of chitosan oligosaccharide also has certain preventive effect.
The composition of Acibenzolar and chitosan oligosaccharide can significantly improve preventive effect and volume increase, can solve the problem such as resistance and cost in current agricultural chemicals single dose application process.
Summary of the invention
For improving plant diseases prevention and volume increase, the invention provides the composition containing Acibenzolar and chitosan oligosaccharide and application technology.
Containing the composition of Acibenzolar and chitosan oligosaccharide, be characterised in that the weight ratio of Acibenzolar and chitosan oligosaccharide is 1:0.05-10.Preferably, the weight ratio of chitosan oligosaccharide and Acibenzolar is 1:0.125-8.Preferred further, the weight ratio of chitosan oligosaccharide and Acibenzolar is 1:0.2-5.
The invention provides the purposes of above-mentioned composition, it is characterized in that the composition of Acibenzolar and chitosan oligosaccharide for controlling disease, promote to increase production.
The purposes of above-mentioned composition, it is characterized in that described disease is the fungus-caused downy mildew of Mastigomycotina, late blight, peronospora tabacina etc., the fungus-caused powdery mildew of Ascomycotina, stalk break, head blight, leaf spot etc., the rust that Basidiomycotina causes, smut etc., the fungus-caused bakanae disease of Fungi Imperfecti, scab etc., the wheat rosette stunt that virus disease causes, Maize Dwarf disease, black streaked dwarf virus of rice etc.Preferably, described disease is downy mildew, powdery mildew, rust, stalk break.
The invention provides the application method of above-mentioned composition, it is characterized in that the application dose of composition is 5-100mg/L.Preferably, the application dose of the composition containing Acibenzolar and chitosan oligosaccharide is 10-50mg/L.
The invention provides the application method of above-mentioned composition, it is characterized in that composition being adopted spraying to plant and filling with at least one process in root, again use the composition identical or different with application concentration first 0-3 time every 7-20d, the application dose of composition is 5-100mg/L.
The application method of above-mentioned composition, described plant refers to be directly or indirectly human needs and any plant of cultivating, comprise vegetables, fruits and vegetables, grain and cotton, lawn, medicinal plant, as potato, tomato, citrus, banana, pears, grape, mango, lichee, longan, apple, peach, apricot, kiwi fruit, strawberry, cherry, rape, watermelon, cucumber, sponge gourd, balsam pear, wax gourd, capsicum, pimento, eggplant, wheat, paddy rice, corn, tealeaves, cotton etc.Preferably, plant is cucumber, wheat, rape, grape, apple, strawberry, watermelon, sponge gourd, balsam pear, wax gourd.
In the present invention, chitosan oligosaccharide is Glucosamine is the mixture of the high molecular polymer of 2-30 by the degree of polymerization that Isosorbide-5-Nitrae-glycosidic bond is formed by connecting, and mean molecule quantity is 1000-3000 dalton.Chitosan oligosaccharide can be prepared by any means, such as following method: the shitosan of deacetylation more than 70% is the mixture of the chitosan oligomer of 2-30 by the degree of polymerization that the methods such as physical degradation methods, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation method prepare.Or, obtain chitin by extracting from fungal cell wall and be the mixture of the chitosan oligomer of 2-30 by the degree of polymerization that the methods such as physical degradation methods, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation method prepare.Or, by by Glucosamine by the degree of polymerization that Isosorbide-5-Nitrae-glycosidic bond chemosynthesis is polymerized being the mixture of the polymer of 2-30.Current chitosan oligosaccharide is the mixture of the high molecular polymer of different polymer, be not that is the oligosaccharides form of the single degree of polymerization in chitosan oligosaccharide, but the chitosan oligosaccharide monose of often kind of degree of polymerization all may exist.So no matter by which kind of method prepared; or by the commercially available prod that above-mentioned preparation method prepares; as long as it is that Glucosamine is by 1; the mixture that the degree of polymerization that 4-glycosidic bond is formed by connecting is 2-30, mean molecule quantity is the daltonian high molecular polymer of 1000-3000 is exactly chitosan oligosaccharide of the present invention; it can contain acetyl group; but the sugar unit of bonding acetyl group is no more than the amount of 40%, preferably more than the amount of 30%, especially preferably more than 20% amount.Chitosan oligosaccharide commercially available is at present all chitosan oligosaccharide product that above-mentioned chitosan oligosaccharide term is set forth substantially.
Beneficial effect of the present invention:
Composition containing Acibenzolar and chitosan oligosaccharide in diseases prevention, promote plant growing way, reach effect of increasing production significantly.
Embodiment
In order to understand the present invention, further illustrate the present invention with embodiment below, but be not limited to the present invention.
Embodiment 1 composition is to the control efficiency of cucumber downy mildew
Single compound or composition dissolution with solvents are prepared into the mother liquor of 1000mg/L.According to biological activity determination trial test, a certain amount of above-mentioned mother liquor 0.1% Tween 80 is diluted, is mixed with the concentration of needs.Carried out cucumber downy mildew pot experiment by chitosan oligosaccharide composition, plant, substantially in 4 leaf phases, is investigated downy mildew incidence, and establishes corresponding single dose and blank before the 1st medicine.Adopt the conventional conventional spray of manual sprayer, every medicament sprays 10 strains, sprays 1 time after the 1st spraying every 7-10d again.10-14d investigation after 2nd medicine, whole blade is investigated in every strain, and record investigation total number of sheets, the sick number of sheets, calculate disease index and preventive effect according to following stage division.
Blade staging:
0 grade: anosis
1 grade: the scab area that is connected accounts for less than 1% of whole leaf area;
3 grades: the scab area that is connected accounts for the 1%-10% of whole leaf area;
5 grades: the scab area that is connected accounts for the 11%-20% of whole leaf area;
7 grades: the scab area that is connected accounts for the 21%-50% of whole leaf area;
9 grades: the scab area that is connected accounts for more than 51% of whole leaf area;
The preventive effect of the active component A that x is working concentration when being a, the preventive effect of the active component B that y is working concentration when being b.Relatively theoretical effect and actual effect, evaluates the synergy of composition, for synergistic effect when being greater than theoretical preventive effect with actual preventive effect.The results are shown in Table 1.
Table 1 composition is to the control efficiency of cucumber downy mildew
Composition containing Acibenzolar and chitosan oligosaccharide finds (see table 1) potted plant test of cucumber downy mildew, when composition proportioning is 1:8-8:1, composition to the actual diseases prevention of cucumber downy mildew all higher than the theoretical preventive effect of correspondent composition.When composition is with during 20-50mg/L during spray application, composition has higher preventive effect.Obviously, be synergy containing the composition of Acibenzolar and chitosan oligosaccharide to cucumber downy mildew.
Embodiment 2 composition is to the control efficiency of wheat diseases
Single compound or composition dissolution with solvents are prepared into the mother liquor of 1000mg/L.According to biological activity determination trial test, a certain amount of above-mentioned mother liquor 0.1% Tween 80 is diluted, is mixed with the concentration of needs.During test, the shooting stage is selected to have the different fields of white powder disease and wheat rust respectively.Adopt the single dose of manual sprayer same concentrations and each 1 time of the conventional conventional spray of composition respectively, every chemicals treatment sprays 20 strains, and establishes blank.Spray again 1 time every 7-10d after medicine.7-10d investigation after 2nd dispenser.Powdery mildew investigates the whole blade of every strain, leaf becomes rusty every strain investigation 3, top leaf, account for the percentage classification of whole leaf area with lesion area on every sheet leaf, carry out the classification of blade powdery mildew according to embodiment 1 method and following methods carries out the classification of blade rust, and calculate disease index and preventive effect.
Blade staging:
0 grade: anosis
1 grade: lesion area accounts for less than 5% of whole leaf area;
3 grades: lesion area accounts for the 6%-25% of whole leaf area;
5 grades: lesion area accounts for the 26%-50% of whole leaf area;
7 grades: lesion area accounts for the 51%-75% of whole leaf area;
9 grades: lesion area accounts for more than 76% of whole leaf area;
Composition to the control efficiency of wheat powdery mildew and wheat rust in table 2 and table 3.
Table 2 composition is to the effect of wheat powdery mildew
Table 3 composition is to the effect of wheat rust
From table 2, composition containing Acibenzolar and chitosan oligosaccharide is when proportioning is 1:5,1:1,5:1, when composition and corresponding single dose concentration are 30mg/L, composition all higher than the preventive effect of the single dose under same concentration, illustrates that composition is synergy to the preventive effect of wheat powdery mildew.
From table 3, composition containing Acibenzolar and chitosan oligosaccharide is when proportioning is 1:3,1:1,3:1, when composition and corresponding single dose concentration are 28mg/L, composition all higher than the preventive effect of the single dose under same concentration, illustrates that composition is synergy to the preventive effect of wheat rust.
Embodiment 3 composition is to the preventive effect of rape disease and effect of increasing production
Experimental field use sufficient base fertilizer and division of cells, list, even uniform.Every community 10m
2, every community appropriate running water of identical amount waters rape seedbed, in order to avoid get seedling to hinder root, Transplanting Density is advisable with every community 100 strain.Respectively in tri-leaf period, flower early stage, with in a few days carrying out specific aim spraying and each 1 time of root irrigation with corresponding medicament, every community root spraying volume is all identical with concentration, the preventive effect of 7-10d investigation sclerotinia rot of colza after medicine.7 ~ 10d after 2nd dispenser, often process is by 10 investigation, often 5 strains, and sclerotinia rot of colza classification and statistics are carried out as follows.Gather in the crops when in yellow green, in the middle part of main shaft, angle fruit is in loquat look 2/3 jiao of fruit, the rape seed weight of different districts of weighing.Period, other control measures of each community are all identical, as moisture, weeding etc.Composition the results are shown in Table 4 to the preventive effect of sclerotinia rot of colza and effect of increasing production.
Blade staging:
0 grade: anosis;
1 grade: slightly fall ill, onset area accounts for less than 5% of stem area;
3 grades: slightly fall ill, onset area accounts for the 6%-15% of stem area;
5 grades: medium morbidity, onset area accounts for the 16%-30% of stem area;
7 grades: highly fall ill, onset area accounts for the 31%-50% of stem area;
9 grades: seriously fall ill, onset area accounts for more than 51% of stem area;
Table 4 composition is to the effect of sclerotinia rot of colza and output
As known from Table 4, when spray amount is 40mg/L and filling root amount 10mg/L, containing the compositions-treated of Acibenzolar and chitosan oligosaccharide to the effect of sclerotinia rot of colza and output all apparently higher than the effect of single dose, illustrate that the composition containing Acibenzolar and chitosan oligosaccharide has obvious disease-resistant synergy and effect of increasing production.
Found by similar method spray test, containing the composition of Acibenzolar and chitosan oligosaccharide when 5-100mg/L, to the powdery mildew of grape, apple, strawberry, watermelon, sponge gourd, balsam pear, wax gourd and the preventive effect of downy mildew all higher than the effect of single dose.