CN102613200B - Application of chitosan oligosaccharide in increasing number of flower buds - Google Patents
Application of chitosan oligosaccharide in increasing number of flower buds Download PDFInfo
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- CN102613200B CN102613200B CN201210059269.8A CN201210059269A CN102613200B CN 102613200 B CN102613200 B CN 102613200B CN 201210059269 A CN201210059269 A CN 201210059269A CN 102613200 B CN102613200 B CN 102613200B
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to an application of chitosan oligosaccharide in increasing the number of flower buds. The invention also provides a method by utilizing a composition containing the chitosan oligosaccharide to promote the differentiation of flower buds or increasing the number of the flower buds, which is characterized in that the composition is used for treating crops at different development stages of a current year or a previous year, wherein the composition is applied at a seedling stage or in the germination period, in a period before the flowering of the crops, in the flowering period of the crops or in the period after the flowering of the crops, or the composition is sequentially applied at the seedling stage or in the germination period, in the period before the flowering, in the flowering period and in the period after the flowering of the crops, and the weight percentage of the chitosan oligosaccharide in the composition is 0.0035 to 0.0195 percent. The chitosan oligosaccharide can effectively promote the differentiation of the flower buds or increase the number of the flower buds, and a universal positive effect on promoting the differentiation of the flower buds or increasing the number of the flower buds can be realized.
Description
Technical field
The invention belongs to agricultural chemicals field, concrete, relate to a kind of chitosan oligosaccharide for promoting the purposes of flower bud differentiation or increase flower bud number.
Background technology
Blooming is a qualitative change process on higher plant history of life, is most important mark in plants ' reproduction development process.Plant growth is just converted into bud physiology and structural state by leaf bud physiology and structural state to certain phase, develops into floral organ blank, and this process is called flower bud differentiation.Flower bud differentiation is the most key stage during flowering plant grows.Regulatory mechanism and regulate and control method for plant flower bud differentiation are mainly taked physical method and fertilizer method of adjustment, the method of taking chemical reagent to regulate also has report, for example patent CN101913927A discloses cotton flower bud differentiation agent, adopt indolebutyric acid, methyl α-naphthyl acetate, paclobutrazol, gibberellin, Triiodobenzoic acid, uniconazole P and heteroauxin etc. to mix regulation and control flower bud differentiation, promote flower bud differentiation or increase flower bud number.
The regulate and control method of the flower bud differentiation of most of flowering plant adopts fertilizer to supply with nutrition more, ensure that crop alimentary is comprehensive, but effect is limited, and does not generally promote the meaning of flower bud differentiation.
Also have by the regulating of plant hormone, but crop is not unique, some inhibition flower bud differentiation, some promotion flower bud differentiation, for example gibberellin suppresses the flower bud differentiation of red bayberry, oranges and tangerines, and whether growth hormone can promote that flower bud differentiation of crop depends on the adjustment of consumption, and abscisic acid will be seen the opportunity of using, the opportunity of using is bad, can suppress flower bud differentiation, etc., can find out, the effect complexity of plant hormone to flower bud differentiation, uncertain also unstable.
Current agriculture related researcher can be to the effective agricultural chemicals of generality flower bud differentiation in active research.The present inventor has found that chitosan oligosaccharide has important facilitation to flower bud differentiation, can promote flower bud differentiation or increase bud quantity, and ensureing the quality of bud.
Summary of the invention
For solving the problems of the technologies described above, the invention provides chitosan oligosaccharide for promoting purposes and the method for flower bud differentiation or increase flower bud number.
Particularly, the invention provides chitosan oligosaccharide for promoting the purposes of flower bud differentiation or increase flower bud number.
The present invention also provides the composition that comprises chitosan oligosaccharide for promoting the purposes of flower bud differentiation or increase flower bud number.
Particularly, the present invention also provides a kind of composition that contains chitosan oligosaccharide that promotes flower bud differentiation or increase flower bud number, described composition comprises chitosan oligosaccharide, auxiliary agent and/or carrier, wherein to account for the percentage by weight of composition total weight be 10.5-20.5% to chitosan oligosaccharide, preferably, to account for the percentage by weight of composition total weight be 12-20% to chitosan oligosaccharide.
The present invention also provides above-mentioned composition for promoting flower bud differentiation or increasing the purposes of flower bud number, it is characterized in that above-mentioned composition is diluted to 300-5000 is applied to crop to promote flower bud differentiation or to increase flower bud number after doubly.
Particularly, the invention provides a kind ofly for promoting flower bud differentiation or increasing the composition pesticide of flower bud number, in this composition pesticide, comprise chitosan oligosaccharide, the percentage by weight that chitosan oligosaccharide accounts for this composition pesticide is 0.0035%-0.0195%.
Above-mentioned this composition pesticide can be prepared liquid preparation or solid pharmaceutical preparation.Liquid preparation can be any liquid preparation of agriculture use, and solid pharmaceutical preparation is also any solid pharmaceutical preparation of agriculture use.Preferably, liquid preparation is aqua, microemulsion, water dispersible granules or suspending agent; Solid pharmaceutical preparation is preferably wetting powder or granule.
The present invention also provides the composition pesticide that comprises chitosan oligosaccharide for promoting the purposes of flower bud differentiation or increase flower bud number, and in the composition pesticide that it is characterized in that comprising chitosan oligosaccharide, the percentage by weight of chitosan oligosaccharide is 0.0035%-0.0195%.
The present invention also provides and has applied the composition that comprises chitosan oligosaccharide for promoting the method for flower bud differentiation or increase flower bud number, it is characterized in that to crop then or the previous year grow each stage set of applications compound crop is processed, be included in crops seedling stage or germination period is used composition, or before crop flowers, use composition, or use composition in the crop flowers phase, or after crop flowers, use composition, or at seedling stage or germination period, before blooming, flowering stage, after blooming, use successively composition, in wherein said composition, the percentage by weight of chitosan oligosaccharide is 0.0035%-0.0195%.
Wherein seedling stage or germination period, before blooming, use the composition that comprises chitosan oligosaccharide and be the flower bud differentiation for promoting flowering plant then and increase flower bud number, flowering stage or bloom after use chitosan oligosaccharide composition for promoting the flower bud differentiation of Second Year flowering plant, before being seedling stage the main opportunity of the flower bud differentiation of annual flowering plant and blooming, use the composition that comprises chitosan oligosaccharide, perennial flowering plant is for promoting flower bud differentiation, after can and blooming in flowering stage the previous year, use chitosan oligosaccharide composition, or before blooming then, use chitosan oligosaccharide composition, before blooming then, using chitosan oligosaccharide composition main purpose is to ensure that the bud having broken up can strong Cheng Lei.
In said method, described composition can be prepared liquid preparation or solid pharmaceutical preparation.Liquid preparation can be any liquid preparation of agriculture use, and solid pharmaceutical preparation is also any solid pharmaceutical preparation of agriculture use.Preferably, liquid preparation is aqua, microemulsion, water dispersible granules or suspending agent; Solid pharmaceutical preparation is preferably wetting powder or granule.In composition, except containing chitosan oligosaccharide, can add arbitrarily auxiliary agent and solvent.
The composition crop that the present invention also provides application to comprise chitosan oligosaccharide promotes flower bud differentiation or increases the method for flower bud number, it is characterized in that at seedling stage of crop or germination period crop applying composition, after using first, used again at interval of 6-19 days, the number of times of using is again 0-2 time, the concentration of at every turn using is identical or different with the concentration of using first, and in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.0035%-0.0195%.
The composition crop that the present invention also provides application to comprise chitosan oligosaccharide promotes flower bud differentiation or increases the method for flower bud number, it is characterized in that before the blooming of crop crop applying composition, after using first, used again at interval of 6-19 days, the number of times of using is again 0-2 time, the concentration of at every turn using is identical or different with the concentration of using first, and in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.0035%-0.0195%.
The composition crop that the present invention also provides application to comprise chitosan oligosaccharide promotes flower bud differentiation or increases the method for flower bud number, it is characterized in that flowering stage in crop the previous year is to crop applying composition, after using first, used again at interval of 6-19 days, the number of times of using is again 0-2 time, the concentration of at every turn using is identical or different with the concentration of using first, and in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.0035%-0.0195%.
The composition crop that the present invention also provides application to comprise chitosan oligosaccharide promotes flower bud differentiation or increases the method for flower bud number, it is characterized in that after the blooming of crop the previous year crop applying composition, after using first, used again at interval of 6-19 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, and in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.0035%-0.0195%.
The composition crop that the present invention also provides application to comprise chitosan oligosaccharide promotes flower bud differentiation or increases the method for flower bud number, it is characterized in that at seedling stage of crop or germination period crop applying composition, after using first, used again at interval of 6-19 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.0035%-0.0195%, and/or before the blooming of crop applying said compositions, after using first, used again at interval of 6-19 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.0035%-0.0195%, and/or at the applying said compositions in flowering stage of the previous year of crop, after using first, used again at interval of 6-19 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.0035%-0.0195%, and/or after the blooming of crop the previous year applying said compositions, after using first, used again at interval of 6-19 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.0035%-0.0195%.
In such use and method, described crop is flowering plant, preferably, can be annual flowering plant and perennial flowering plant, preferably, crop is selected from eggplant, cucumber, peanut, coconut, balsam pear, pea, wax gourd, pears, banana, pawpaw, green soy bean, mango, watermelon, oranges and tangerines, orange, kiwi fruit, strawberry, pineapple, capsicum, apple, jujube, persimmon, grape, cherry, lichee, muskmelon, potato, pumpkin, peach, cotton, sweet potato, radish, carrot, kidney bean, jowar, tobacco, oil tea, rape, wheat, paddy rice, corn, spinach, sponge gourd, celery, beet, soybean, tea tree, rubber, sugarcane, Kidney bean, garlic, blueberry, Hami melon, Lee, apricot, longan, navel orange, red bayberry, Chinese herbaceous peony, tung oil tree, walnut, chrysanthemum, any in red bean and tender flower stalk.
In said method, any effectively conventional method when method of using refers to the applications of pesticide, includes but not limited to powder injection process, spray-on process, fumigating system, soil treatment method or smog method etc.
Wherein powder injection process refers to the surface that utilizes wind-force that duster produces the agricultural chemicals of pulvis to be sprinkling upon equably to crop or controlling object; Spray-on process refers to a certain amount of agricultural chemicals and appropriate water and is made into liquid, medicine liquid spray become to droplet with spraying apparatus, is evenly distributed in crop or controlling object surface; Fumigating system refers to and adopts fumigant or volatile medicament, makes its volatilization become the effectively gas of prevention and elimination of disease and pests; Soil treatment method refers to and takes to spray, dusts, spreads the methods such as pesticide-clay mixture, and agricultural chemicals is imposed in ground or certain soil layer equably; Smog method refers to utilizes special facility that oily pesticide dispersion is become to smoke condition, to reach the method for Pesticidal and sterilizing weeding.
In the present invention, chitosan oligosaccharide is the mixture of the degree of polymerization that Glucosamine is formed by connecting by the Isosorbide-5-Nitrae-glycosidic bond high molecular polymer that is 2-30, and mean molecule quantity is 1000-3000 dalton.Chitosan oligosaccharide can be prepared by any means, such as following method: the mixture of the chitosan oligomer that the degree of polymerization that the shitosan of deacetylation more than 70% prepares by methods such as mechanical degradation method, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation methods is 2-30.Or, obtain chitin and the mixture of the chitosan oligomer that the degree of polymerization for preparing by methods such as mechanical degradation method, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation methods is 2-30 by extracting from fungal cell wall or insect body wall.Or, the mixture of the polymer that is 2-30 by the degree of polymerization being polymerized by the chemosynthesis of Isosorbide-5-Nitrae-glycosidic bond by Glucosamine.Chitosan oligosaccharide is the mixture of the high molecular polymer of different polymer at present, that is to say in chitosan oligosaccharide to be not the oligosaccharides form of the single degree of polymerization, but the chitosan oligosaccharide monose of every kind of degree of polymerization all may exist.So no matter by which kind of method prepare; or the commercially available prod preparing by above-mentioned preparation method; as long as it is that Glucosamine passes through 1; the degree of polymerization that 4-glycosidic bond is formed by connecting is that 2-30, mean molecule quantity are that the mixture of the daltonian high molecular polymer of 1000-3000 is exactly chitosan oligosaccharide of the present invention; it can contain acetyl group; but the sugar unit of bonding acetyl group is no more than 40% amount, be preferably no more than 30% amount, be especially preferably no more than 20% amount.The chitosan oligosaccharide product that commercially available chitosan oligosaccharide is all set forth for above-mentioned chitosan oligosaccharide term substantially at present.
In such use and method, described promotion flower bud differentiation or increase flower bud number and refer to and promote that the bud of crop effectively breaks up, the bud quantity that this can Cheng Lei increases.The following examples of the present invention check chitosan oligosaccharide to promoting flower bud differentiation and increasing the concrete effect of flower bud number by the effective bud quantity of checking (namely can Cheng Lei bud quantity).
Beneficial effect of the present invention:
The present invention has found that chitosan oligosaccharide can effectively promote flower bud differentiation or increase flower bud number, and promotes flower bud differentiation or increase flower bud number that flowering plant is had to general advantageous effects, and its effect is not depended on the kind of crop and specialization.
Embodiment
In order to understand the present invention, further illustrate the present invention with embodiment below, but do not limit the present invention.
15wt% chitosan oligosaccharide solution with water is diluted to respectively to corresponding application concentration, concentration is calculated according to weight percent concentration, in outcome record, calculate with ppm (1,000,000/) concentration, what in outcome record below, 50ppm represented is that weight percent concentration is 0.005%, namely by 3000 times of 15wt% chitosan oligosaccharide solution dilutions.Clear water is processed in contrast.The area of the crop of experiment is 50 square metres, and each experiment divides 4 groups, averages and calculates various indexs.If fruit tree, 30 strains are grown up fruit tree as a sample, and 4 processing samples and 1 check sample are tested.
Embodiment 1 sprays chitosan oligosaccharide and promotes flower bud differentiation to increase the experiment of effective flower bud number
Solve the chitosan oligosaccharide solution that sprays variable concentrations (all concentration is taking ppm as unit) in difference, then detect the percentage (processing the number of flowers of crop divided by the number of flowers of clear water contrast) that the number of flowers of the crop of chitosan oligosaccharide solution-treated increases with respect to the number of flowers of clear water processing (the clear water amount of clear water processing is identical with the amount of chitosan oligosaccharide solution), the number of flowers of the crop of clear water processing is defined as 100%.
Repeatedly spray chitosan oligosaccharide solution on same opportunity, represent to spray interval with "/", be 9 days interval time.
Spray the statistics of number of flowers after chitosan oligosaccharide solution for following crop:
Because the workload of statistics bud is concrete, by the statistics of above-mentioned crop number of flowers, confirm that chitosan oligosaccharide can both promote flower bud differentiation effectively to all kinds of flowering plants, increase number of flowers.
Claims (5)
1. chitosan oligosaccharide is for promoting the purposes of flower bud differentiation or increase flower bud number.
2. comprise the composition of chitosan oligosaccharide for promoting the purposes of flower bud differentiation or increase flower bud number.
3. comprise the composition pesticide of chitosan oligosaccharide for promoting the purposes of flower bud differentiation or increase flower bud number, in the composition pesticide that it is characterized in that comprising chitosan oligosaccharide, the percentage by weight of chitosan oligosaccharide is 0.0035%-0.0195%.
4. the composition that application comprises chitosan oligosaccharide is for promoting the method for flower bud differentiation or increase flower bud number, it is characterized in that to crop then or the previous year grow each stage set of applications compound crop is processed, be included in crops seedling stage or germination period is used composition, or before crop flowers, use composition, or use composition in the crop flowers phase, or after crop flowers, use composition, or at seedling stage or germination period, before blooming, flowering stage, after blooming, use successively composition, in wherein said composition, the percentage by weight of chitosan oligosaccharide is 0.0035%-0.0195%.
5. the composition crop that application comprises chitosan oligosaccharide promotes flower bud differentiation or increases the method for flower bud number, it is characterized in that at seedling stage of crop or germination period crop applying composition, after using first, used again at interval of 6-19 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.0035%-0.0195%, and/or before the blooming of crop applying said compositions, after using first, used again at interval of 6-19 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.0035%-0.0195%, and/or at the applying said compositions in flowering stage of the previous year of crop, after using first, used again at interval of 6-19 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.0035%-0.0195%, and/or after the blooming of crop the previous year applying said compositions, after using first, used again at interval of 6-19 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.0035%-0.0195%.
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| CN105016895A (en) * | 2015-08-05 | 2015-11-04 | 青岛苏贝尔作物营养有限公司 | Water-soluble fertilizer for mango flower forcing and application method |
| CN105210654B (en) * | 2015-10-30 | 2016-05-04 | 山东省农业科学院蔬菜花卉研究所 | A kind of method that promotes Melon Female flower differentiation |
| CN112021027B (en) * | 2020-09-09 | 2022-03-29 | 山东爱果者生物技术有限公司 | Method for improving stress resistance of plants and promoting flower bud differentiation |
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| CN1809274A (en) * | 2003-04-22 | 2006-07-26 | 生物农业股份有限公司 | Use of lipo chitooligosaccharides to initiate early flowering and fruit development in plants and related methods and compositions. |
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| CN1809274A (en) * | 2003-04-22 | 2006-07-26 | 生物农业股份有限公司 | Use of lipo chitooligosaccharides to initiate early flowering and fruit development in plants and related methods and compositions. |
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