CN102613192B - Application of chitosan oligosaccharides in promotion of shortness and stoutness of new branches - Google Patents
Application of chitosan oligosaccharides in promotion of shortness and stoutness of new branches Download PDFInfo
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Abstract
The invention relates to application of chitosan oligosaccharides to promotion of shortness and stoutness of new branches. The invention also provides a method for promoting shortness and stoutness of new branches of fruit trees or woods by applying a composition containing chitosan oligosaccharides. The method is characterized by treating the fruit trees or woods by applying the composition at each stage in the year before the fruit trees or woods develop or in the current year, including applying the composition in the seedling stage of the fruit trees or the germination stage of the adult trees, or applying the composition before the fruit trees or woods blossom, or applying the composition in the blossom of the fruit trees or woods, or applying the composition after the fruit trees or woods blossom or applying the composition in the seeding stage in the previous year or the current year or in the germination stage of the adult trees, before blossoming, in the blossom and after blossoming in sequence, wherein the weight percent of chitosan oligosaccharides in the composition is 0.005-0.0175%. The method has the following beneficial effects: chitosan oligosaccharides can effectively improve the quality of the new branches of the fruit trees or woods and promote the new branches to grow towards shortness and stoutness.
Description
Technical field
The invention belongs to agricultural chemicals field, concrete, relate to a kind of chitosan oligosaccharide for promoting the short and thick strong purposes of the newborn branch of fruit tree or forest.
Background technology
The growth quality of the newborn branch of fruit tree directly affect then or the Flowering and fruiting of next year, if the newborn branch of fruit tree a little less than, easily disconnected when bearing fruit, or supply with malnutrition, cause fruit quality decline.If fruit tree or forestry are small and weak with the newborn branch of tree, when applying so it and carry out cutting propagation, to breed of low qualityly, survival rate is low, a little less than the bad ability of coercing environment of new sapling opposing.
The method that can control at present newborn branch growth quality normally adopts the mode of artificial pruning, result value and nutrition supply is worth to little branch and prunes, to promote growing up strong and sturdy of newborn branch.
In addition, for ensureing the quality of cutting propagation sapling, generally choose the branch of tubbiness and breed, can guarantee the quality of new sapling, but because newborn branch quality is low, the cuttage bar of choosing high-quality is very difficult.
The present invention has found to promote newborn branch quality, namely makes the short and thick strong agricultural chemicals of newborn branch and promotes the short and thick strong purposes of newborn branch.
Summary of the invention
For solving the problems of the technologies described above, the invention provides chitosan oligosaccharide for promoting the newborn short and thick strong purposes of branch of fruit tree or forest and method.
Particularly, the invention provides chitosan oligosaccharide for promoting the short and thick strong purposes of the newborn branch of fruit tree or forest.
The present invention also provides the composition that comprises chitosan oligosaccharide for promoting the short and thick strong purposes of the newborn branch of fruit tree or forest.
Particularly, the invention provides and a kind ofly for promoting the short and thick strong composition pesticide of the newborn branch of fruit tree or forest, in this composition pesticide, comprise chitosan oligosaccharide, the percentage by weight that chitosan oligosaccharide accounts for this composition pesticide is 0.005%-0.0175%.
Above-mentioned this composition pesticide can be prepared liquid preparation or solid pharmaceutical preparation.Liquid preparation can be any liquid preparation of agriculture use, and solid pharmaceutical preparation is also any solid pharmaceutical preparation of agriculture use.Preferably, liquid preparation is aqua, microemulsion, water dispersible granules or suspending agent; Solid pharmaceutical preparation is preferably wetting powder or granule.
The present invention also provides the composition pesticide that comprises chitosan oligosaccharide for promoting the short and thick strong purposes of the newborn branch of fruit tree or forest, and in the composition pesticide that it is characterized in that comprising chitosan oligosaccharide, the percentage by weight of chitosan oligosaccharide is 0.005%-0.0175%.
The present invention also provides and has applied the composition that comprises chitosan oligosaccharide for promoting the short and thick strong method of the newborn branch of fruit tree or forest, it is characterized in that the previous year that fruit tree or forest are grown or then each stage set of applications compound fruit tree or forest are processed, the seedling stage or the bearing tree germination period that are included in fruit tree are used composition, or before blooming, fruit tree or forest use composition, or use composition flowering stage in fruit tree or forest, or after blooming, fruit tree or forest use composition, or at the previous year or seedling stage then or bearing tree germination period, before blooming, flowering stage, after blooming, use successively composition, in wherein said composition, the percentage by weight of chitosan oligosaccharide is 0.005%-0.0175%.
In said method, described composition can be prepared liquid preparation or solid pharmaceutical preparation.Liquid preparation can be any liquid preparation of agriculture use, and solid pharmaceutical preparation is also any solid pharmaceutical preparation of agriculture use.Preferably, liquid preparation is aqua, microemulsion, water dispersible granules or suspending agent; Solid pharmaceutical preparation is preferably wetting powder or granule.In composition, except containing chitosan oligosaccharide, can add arbitrarily auxiliary agent and solvent.
The present invention also provides and has applied the composition that comprises chitosan oligosaccharide for promoting the short and thick strong method of the newborn branch of fruit tree or forest, it is characterized in that, at seedling stage or the bearing tree germination period of fruit tree or forest, fruit tree or forest are used to composition, after using first, at interval of 7-21 days, use again, the number of times of using is again 0-2 time, the concentration of at every turn using is identical or different with the concentration of using first, and in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.005%-0.0175%.
The present invention also provides and has applied the composition that comprises chitosan oligosaccharide for promoting the short and thick strong method of the newborn branch of fruit tree or forest, it is characterized in that, before the previous year of fruit tree or forest or blooming then, fruit tree or forest are used to composition, after using first, at interval of 7-21 days, use again, the number of times of using is again 0-2 time, the concentration of at every turn using is identical or different with the concentration of using first, and in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.005%-0.0175%.
The present invention also provides and has applied the composition that comprises chitosan oligosaccharide for promoting the short and thick strong method of the newborn branch of fruit tree or forest, it is characterized in that, in the previous year of fruit tree or forest or flowering stage then, fruit tree or forest are used to composition, after using first, at interval of 7-21 days, use again, the number of times of using is again 0-2 time, the concentration of at every turn using is identical or different with the concentration of using first, and in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.005%-0.0175%.
The present invention also provides and has applied the composition that comprises chitosan oligosaccharide for promoting the short and thick strong method of the newborn branch of fruit tree or forest, it is characterized in that, after the previous year of fruit tree or forest or blooming then, fruit tree or forest are used to composition, after using first, at interval of 7-21 days, use again, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, and in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.005%-0.0175%.
The present invention also provides and has applied the composition that comprises chitosan oligosaccharide for promoting the short and thick strong method of the newborn branch of fruit tree or forest, it is characterized in that, at seedling stage or the bearing tree germination period of fruit tree or forest, fruit tree or forest are used to composition, after using first, at interval of 7-21 days, use again, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.005%-0.0175%, and/or before the previous year of fruit tree or forest or blooming then applying said compositions, after using first, at interval of 7-21 days, use again, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.005%-0.0175%, and/or at the previous year of fruit tree or forest or applying said compositions in flowering stage then, after using first, at interval of 7-21 days, use again, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.005%-0.0175%, and/or, applying said compositions after the previous year of fruit tree or forest or blooming then, after using first, at interval of 7-21 days, use again, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.005%-0.0175%.
In such use and method, described fruit tree or forest are selected any to the valuable fruit tree of the mankind and forest, comprise and view and admire forest and commodity trees etc., and preferred, fruit tree and forest are selected from walnut, jujube, ginkgo, peach, Chinese torreya, coconut, Chinese chestnut, persimmon, cherry, banana, orange, kiwi fruit, grape, pine tree, lichee, cashew nut, willow, willow, apple, mango, oranges and tangerines, pawpaw, pears, pomegranate, Lee, red bayberry, tangerine, loquat, olive, longan, elder, idesia, Chinese pistache, Garcinia multiflora, oil tea, olive, tung oil tree, the bark of eucommia, the bark of official magnolia, dogwood, Chinese cassia tree, citron, the sterculia seed, cape jasmine, sea-buckthorn, Buddha's hand, nutmeg, anistree, Chinese prickly ash, clore basil, bay, cloves, Jasmine, pepper, Chinese toon, matrimony vine, Lei Zhu, tea tree, Cortex Ilicis Rotundae, fig, masson pine, wet-land pine tree, torch pine, Korean pine, black pine, Chi Shan, metasequoia, paulownia, locust tree, eucalyptus, mao bamboon, shrubby flase indigo, ash tree, purple willow, lacquer tree, blue or green money willow, wild jujube, bamboo grows, any in mulberry tree and fibert
In said method, any effectively conventional method when method of using refers to the applications of pesticide, includes but not limited to powder injection process, spray-on process, fumigating system, soil treatment method or smog method etc.
Wherein powder injection process refers to the surface that utilizes wind-force that duster produces the agricultural chemicals of pulvis to be sprinkling upon equably to fruit tree or forest or controlling object; Spray-on process refers to a certain amount of agricultural chemicals and appropriate water and is made into liquid, medicine liquid spray become to droplet with spraying apparatus, is evenly distributed in fruit tree or forest or controlling object surface; Fumigating system refers to and adopts fumigant or volatile medicament, makes its volatilization become the effectively gas of prevention and elimination of disease and pests; Soil treatment method refers to and takes to spray, dusts, spreads the methods such as pesticide-clay mixture, and agricultural chemicals is imposed in ground or certain soil layer equably; Smog method refers to utilizes special facility that oily pesticide dispersion is become to smoke condition, to reach the method for Pesticidal and sterilizing weeding.
In the present invention, chitosan oligosaccharide is the mixture of the degree of polymerization that Glucosamine is formed by connecting by the Isosorbide-5-Nitrae-glycosidic bond high molecular polymer that is 2-30, and mean molecule quantity is 1000-3000 dalton.Chitosan oligosaccharide can be prepared by any means, such as following method: the mixture of the chitosan oligomer that the degree of polymerization that the shitosan of deacetylation more than 70% prepares by methods such as mechanical degradation method, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation methods is 2-30.Or, by extracting, obtain chitin and the mixture of the chitosan oligomer that the degree of polymerization for preparing by methods such as mechanical degradation method, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation methods is 2-30 from fungal cell wall or insect body wall.Or, the mixture of the polymer that is 2-30 by the degree of polymerization being polymerized by the chemosynthesis of Isosorbide-5-Nitrae-glycosidic bond by Glucosamine.Chitosan oligosaccharide is the mixture of the high molecular polymer of different polymer at present, that is to say in chitosan oligosaccharide to be not the oligosaccharides form of the single degree of polymerization, but the chitosan oligosaccharide monose of every kind of degree of polymerization all may exist.So no matter by which kind of method prepare; or the commercially available prod preparing by above-mentioned preparation method; as long as it is that Glucosamine passes through 1; the degree of polymerization that 4-glycosidic bond is formed by connecting is that 2-30, mean molecule quantity are that the mixture of the daltonian high molecular polymer of 1000-3000 is exactly chitosan oligosaccharide of the present invention; it can contain acetyl group; but the sugar unit of bonding acetyl group is no more than 40% amount, be preferably no more than 30% amount, be especially preferably no more than 20% amount.The chitosan oligosaccharide product that commercially available chitosan oligosaccharide is all set forth for above-mentioned chitosan oligosaccharide term substantially at present.
In such use and method, the short and thick strong newborn branch that refers to chitosan oligosaccharide composition promotion fruit tree of the newborn branch of described promotion fruit tree or forest is grown up slowly in length, grow up fast, and the density of newborn branch is high at diametrically.
Beneficial effect of the present invention:
The present invention has found that chitosan oligosaccharide can improve the quality of the newborn branch of fruit tree or forest effectively, promotes newborn branch to grow up towards short and thick strong orientation direction.This influence is not only specific fruit tree or forest, but all produces effect for all fruit trees and forest, and effective object has generality.
Embodiment
In order to understand the present invention, with embodiment, further illustrate the present invention below, but do not limit the present invention.
5wt% chitosan oligosaccharide solution with water is diluted to respectively to corresponding application concentration, concentration is calculated according to weight percent concentration, in outcome record, with ppm (1,000,000/) concentration, calculate, what in outcome record below, 50ppm represented is that weight percent concentration is 0.005%, namely by 2000 times of 10wt% chitosan oligosaccharide solution dilutions.Clear water is processed in contrast.The fruit tree of each experiment sample or the strain of forest tree is 40 strains, comprises fruit tree or forest in seedling stage, also comprises fruit tree or forest in the manhood, and ordinary circumstance accounts for 25%, approximately 10 strains left and right in seedling stage.Each experiment repeats 4 samples, and with fresh water spraying in contrast, fresh water spraying amount is identical with the chitosan oligosaccharide solution amount of spraying.At one, repeatedly spray period, in form, by "/", represent interval, be 10 days interval.
Embodiment 1 sprays the impact of the newborn shoot growth of chitosan oligosaccharide on fruit tree or forest
For obtaining comparative result intuitively, the numerical value of measuring take the contrast of clear water processing, as contrast, carries out percentage calculation.The index of chitosan oligosaccharide processing represents with percentage, as diameter × 100% of the newborn branch of diameter/clear water control treatment of the newborn branch of contrast percentage=chitosan oligosaccharide processing of the diameter of new branch.
Processing for seedling stage (namely the age of tree is less than 2 years) all adopts the spray time identical with bearing tree, for example bearing tree sprays chitosan oligosaccharide solution after blooming, although so seedling stage trees do not open colored, process time identical, carry out with the time synchronized of bearing tree.
The Measuring Time of the indices of shoot is that New shoot growth starts to measure for 20 days, measures the metering system of taking naked branch, removes blade and leaf bud.
The statistics that sprays newborn branch diameter after chitosan oligosaccharide solution, length and density for following fruit tree or forest, statistical data adopts mean value calculation:
From the statistics of upper table, can find out, measured several planting fruit-trees and the shoot diameter of forest increase, contraction in length, and the density of shoot also increases, so chitosan oligosaccharide is realized the short and thick strong facilitation of shoot for the processing of fruit tree and forest.
Claims (6)
1. the composition that application comprises chitosan oligosaccharide is for promoting the short and thick strong method of the newborn branch of fruit tree or forest, it is characterized in that the previous year that fruit tree or forest are grown or then each stage set of applications compound fruit tree or forest are processed, the seedling stage or the bearing tree germination period that are included in fruit tree are used composition, or before blooming, fruit tree or forest use composition, or use composition flowering stage in fruit tree or forest, or after blooming, fruit tree or forest use composition, or at the previous year or seedling stage then or bearing tree germination period, before blooming, flowering stage, after blooming, use successively composition, in wherein said composition, the percentage by weight of chitosan oligosaccharide is 0.005%-0.0175%.
2. the composition that application comprises chitosan oligosaccharide is for promoting the short and thick strong method of the newborn branch of fruit tree or forest, it is characterized in that, at seedling stage or the bearing tree germination period of fruit tree or forest, fruit tree or forest are used to composition, after using first, at interval of 7-21 days, use again, the number of times of using is again 0-2 time, the concentration of at every turn using is identical or different with the concentration of using first, and in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.005%-0.0175%.
3. the composition that application comprises chitosan oligosaccharide is for promoting the short and thick strong method of the newborn branch of fruit tree or forest, it is characterized in that, before the previous year of fruit tree or forest or blooming then, fruit tree or forest are used to composition, after using first, at interval of 7-21 days, use again, the number of times of using is again 0-2 time, the concentration of at every turn using is identical or different with the concentration of using first, and in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.005%-0.0175%.
4. the composition that application comprises chitosan oligosaccharide is for promoting the short and thick strong method of the newborn branch of fruit tree or forest, it is characterized in that, in the previous year of fruit tree or forest or flowering stage then, fruit tree or forest are used to composition, after using first, at interval of 7-21 days, use again, the number of times of using is again 0-2 time, the concentration of at every turn using is identical or different with the concentration of using first, and in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.005%-0.0175%.
5. the composition that application comprises chitosan oligosaccharide is for promoting the short and thick strong method of the newborn branch of fruit tree or forest, it is characterized in that, after the previous year of fruit tree or forest or blooming then, fruit tree or forest are used to composition, after using first, at interval of 7-21 days, use again, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, and in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.005%-0.0175%.
6. the composition that application comprises chitosan oligosaccharide is for promoting the short and thick strong method of the newborn branch of fruit tree or forest, it is characterized in that, at seedling stage or the bearing tree germination period of fruit tree or forest, fruit tree or forest are used to composition, after using first, at interval of 7-21 days, use again, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.005%-0.0175%, and/or before the previous year of fruit tree or forest or blooming then applying said compositions, after using first, at interval of 7-21 days, use again, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.005%-0.0175%, and/or at the previous year of fruit tree or forest or applying said compositions in flowering stage then, after using first, at interval of 7-21 days, use again, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.005%-0.0175%, and/or, applying said compositions after the previous year of fruit tree or forest or blooming then, after using first, at interval of 7-21 days, use again, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, in the composition that comprises chitosan oligosaccharide of at every turn using, the percentage by weight of chitosan oligosaccharide is 0.005%-0.0175%.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1520723A (en) * | 2003-01-30 | 2004-08-18 | 中国科学院上海植物生理研究所 | Chitosan oligosaccharide containging pesticide composition and its application |
| WO2005085444A1 (en) * | 2004-03-03 | 2005-09-15 | National Institute Of Agrobiological Sciences | Chithin oligosaccharide elicitor-binding protein |
| CN101543230A (en) * | 2008-03-28 | 2009-09-30 | 中国科学院大连化学物理研究所 | Plant stress-resistant inducer and application thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1520723A (en) * | 2003-01-30 | 2004-08-18 | 中国科学院上海植物生理研究所 | Chitosan oligosaccharide containging pesticide composition and its application |
| WO2005085444A1 (en) * | 2004-03-03 | 2005-09-15 | National Institute Of Agrobiological Sciences | Chithin oligosaccharide elicitor-binding protein |
| CN101543230A (en) * | 2008-03-28 | 2009-09-30 | 中国科学院大连化学物理研究所 | Plant stress-resistant inducer and application thereof |
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