CN106804605B - A kind of wound healing agent for plants and application thereof containing sodium alginate oligosaccharide - Google Patents
A kind of wound healing agent for plants and application thereof containing sodium alginate oligosaccharide Download PDFInfo
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- CN106804605B CN106804605B CN201510868901.7A CN201510868901A CN106804605B CN 106804605 B CN106804605 B CN 106804605B CN 201510868901 A CN201510868901 A CN 201510868901A CN 106804605 B CN106804605 B CN 106804605B
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Abstract
The present invention relates to a kind of wound healing agent for plants containing sodium alginate oligosaccharide, contain sodium alginate oligosaccharide in the consolidant.It is 0.001%-0.02% that sodium alginate oligosaccharide, which accounts for protectant weight percent,.Also contain one or more of inducer, nutritional agents, fungicide, insecticide, film forming agent.It can be used for spraying or smearing.It uses before and after trimming, band girdle or grafting, wound is handled with the consolidant, wound morbidity can be reduced and promote wound healing.The consolidant is suitable for fruit tree and flowers.
Description
Technical field:
The invention belongs to agricultural technology fields, specially promotion plant wound heeling technical field, and in particular to Yi Zhongzhu
Wanting ingredient is the plant wound healing protective agent of sodium alginate oligosaccharide.
Background technique:
Fruits industry is one of important composition of agricultural economy, and cultivation of fruit tree plantation is there are apical dominance, in actual production
Often by trimming to fruit tree, artificially manufacture high yield is tree-like, improves the yield and quality of fruit, extends the result time limit.This
Outside in order to improve the breed, changes harvest time, improve product quality, increase yield, often fruit tree is grafted.And it trims and grafts
Wound will be formed on fruit tree, the production of growth and development and high-quality fruit to fruit tree brings detrimental effect, main to show
In terms of following two: first is that Mechanical wound causes one of the reason of fruit tree gummosis, such as grape, Kiwi berry, peach, walnut, mandarin orange
Tangerine, lemon etc., as a result lead to that tree vigo(u)r is weak, yield is reduced, the decline of nutrient quality；Second is that the generation of mechanical damage is so that germ
Tree body is more easily invaded, the weak up to dead of tree vigo(u)r is ultimately caused, such as the intrusion of rotten pathogenic bacteria.The above problem constrains fruit tree
The development of industry, at present also there is using copper sulphate and pulverized limestone system fruit tree wound healing mainly using fungicide is smeared in production
Make paste protective agent, there are also use grafting wax, paint or seal and shear oil.Its main function be antibacterial and formed protective layer, however on
The method of stating is of limited application, and application effect is poor, and there is also have residual in plant and ambient enviroment, be not easy to drop after use
Solution generates the problems such as poisoning to surrounding biologic and environment.It is a kind of safe and efficient and with practical value therefore, it is necessary to develop
Wound-healing agent, to solve the problems, such as woods fruit tree wound healing.
Sodium alginate is the natural polysaccharide carbohydrate extracted from the seaweed such as kelp, and sodium alginate oligosaccharide is its degradation
Product is logical by а-L- mannuronic acid (mannuronic acid, M) and b-D- guluronic acid (guluronic acid, G)
The oligosaccharide crossing Isosorbide-5-Nitrae-glucosides key connection and being made of different fragments (MM sections, GG sections and MG sections), small with relative molecular mass,
The advantages that absorbability is strong.Sodium alginate oligosaccharide has certain regulatory function to plant growth and development, to the degeneration-resistant of plant and mentions
High-quality also has remarkable result.But it yet there are no document and patent report currently as wound healing agent for plants research and development application
Road.Sodium alginate oligosaccharide is to have the characteristics that safe and environment-friendly, low-residual from natural products, is used for plant wound heeling
Agent, it is more advantageous for other means.The present invention is in line with prevention and treatment thought that is environmentally protective, putting prevention first, it is intended to mention
For a kind of wound healing agent for plants for meeting environmental requirement, being easily reduced wound morbidity, promoting wound healing.
Summary of the invention:
It is an object of the present invention to provide a kind of wound healing agent for plants containing sodium alginate oligosaccharide.
To achieve the above object, the technical solution of the present invention is as follows:
Wound healing agent for plants of the present invention is a kind of wound healing agent for plants containing sodium alginate oligosaccharide, wherein
Sodium alginate oligosaccharide average molecular weight is 500-5000Da, content 0.001%-0.02%.
Wound healing agent for plants of the present invention also contains plant induced resistance agent, nutritional agents, fungicide, insecticide, film forming
One or more of agent.
Plant induced resistance agent contained in wound healing agent for plants of the present invention is specially oligogalacturonans, average
Molecular weight is 500-3000Da, and accounting for weight percent is 0.005%-0.01%.
Plant nutrient agent contained in wound healing agent for plants of the present invention is specially humic acid, accounts for plant consolidant
Weight percent is 1%-5%.
Fungicide contained in wound healing agent for plants of the present invention be specially matrine, jamaicin, pyrethrin,
One or more of nimbin, rotenone, accounting for plant consolidant weight percent is 0.05%-1%.
Insecticide contained in wound healing agent for plants of the present invention is specially one of jervine, nicotine or several
Kind, accounting for plant consolidant weight percent is 0.05%-1%.
Film forming agent contained in wound healing agent for plants of the present invention be specially chitosan, sodium alginate, carragheen,
One of prolan polysaccharide, Curdlan polysaccharide are a variety of, and accounting for plant consolidant weight percent is 0.5%-5%.
Wound healing agent for plants of the present invention can be made according to actual conditions such as floristics, disease and wounds
Any ointment preparation and liquid preparation, liquid preparation are preferably aqua, microemulsion, suspending agent or moisture content dispersing agent.
Wound healing agent for plants of the present invention can before vegetation pruning, grafting or band girdle 1-3 days or vegetation pruning, transfer
Connect or band girdle after in 3 days, on wound use above-mentioned consolidant.
The present invention have the advantage that and the utility model has the advantages that
(1) wound healing agent for plants provided by the invention has comprehensively considered induction fruit tree resistance, growth promotion, nutrition, suppression
Bacterium, holding moisture etc. promote the various factors of wound healing.It is handled by sodium alginate oligosaccharide, promotes wound location cell
Growth；It is handled by oligogalacturonans, enhances the resistance of fruit tree wound location；It is handled by humic acid, is wound location
Healing sufficient nutrition is provided；It is handled by fungicide, suppresses growth of microorganism and breed；It is handled by insecticide, repellent
Pestinfestation and breeding；It is handled by natural polysaccharides such as chitosan or sodium alginates, control wound location moisture content scatters and disappears；It is comprehensive
More ideal wound healing effect is played, the healing of wound can be accelerated, substantially reduces infecting for rotten pathogenic bacteria, is being carried out
Embodiment in, effect be better than existing commercial reagents treatment effect.
(2) growth accelerator sodium alginate oligosaccharide, inducer oligomerization used in wound healing agent for plants provided by the invention
Galactolipin, film forming agent chitosan, sodium alginate, carragheen, prolan polysaccharide, Curdlan polysaccharide belong to natural products, are made
Fungicide and insecticide belong to plant source source, for promoting wound healing agent for plants in use, no pollution to the environment,
To human body without health hazard.And compared with simple using fungicide protection wound, fungicide usage amount used declines to a great extent.Cause
This is a kind of novel, green wound healing agent for plants.
(3) wound healing agent for plants dosage form multiplicity provided by the present invention, can be before and after vegetation pruning, grafting or band girdle
It uses, has and prevent and treat double effects, plant morbidity is few, and wound healing is fast, and growing way is prosperous.Method of administration is simple, at low cost,
Application easy to spread.Compared with prior art, fruit tree wound healing is solved to endanger slowly, vulnerable to disease pest and influence fruit tree growth
Problem.It is easy to use, effect is good, with good development and application prospects.
Detailed description of the invention
IAA content changes with concentration in Fig. 1 sodium alginate oligosaccharide processing tobacco cell.
IAA content is with the variation for handling the time in Fig. 2 sodium alginate oligosaccharide processing tobacco cell.
Specific embodiment
Below with reference to embodiment, the invention will be further described:
Wound healing effect of the 1. sodium alginate oligosaccharide Wound-healing agent of embodiment to apple tree canker
Sodium alginate oligosaccharide is carried out in Shaanxi Luochuan orchard to test the prevention and treatment of canker of apple fruit.
Formulation content formula are as follows:
Sodium alginate oligosaccharide 0.02g；
Oligogalacturonans 0.05g；
Humic acid 10g；
Nimbin 0.5g；
Jervine 0.5g；
Chitosan: 20g；
Moisturizing is to 1000g
Other three groups of processing are respectively clear water control, 843 rehabilitation agents and the Wound-healing agent for not adding sodium alginate.
120 sick scars are randomly selected in orchard to be tested, and are first scraped off lesion necrosis part, then in sick scar
Four kinds of medicaments of even smearing, and 1-2cm also coats consolidant outside away from sick scar, smears 3 times altogether, 10 days each interval times.With
60d, 180d and 270d count healing effect after treatment afterwards.
The experimental results are shown inthe following table:
Medicament sick scar is struck off after healing result (callus width mm)
The recurrence result of sodium alginate wound healing agent for plants prevention and treatment apple tree canker
The above results show that sodium alginate oligosaccharide wound healing agent for plants has apparent rush to canker of apple fruit wound healing
Into effect, after smearing three times, callus width is handled, is 4.33 times for commonly using 843 rehabilitation agent of medicament processing width in production.
Simultaneously next year rot disease recurrence the result shows that, sodium alginate oligosaccharide wound healing agent for plants smear 3 times processing relative control effect is
96.16%, and as a result 843 rehabilitation agent relative control effects also show and sodium alginate oligosaccharide are not added less than 70%, remaining ingredient is identical
Preparation also has good facilitation to the wound healing of canker of apple fruit.
Wound healing effect of the 2. sodium alginate oligosaccharide Wound-healing agent of embodiment to apple tree canker
Sodium alginate oligosaccharide is carried out in Liaoning Jinzhou orchard to test the prevention and treatment of canker of apple fruit.
Formulation content formula are as follows:
Sodium alginate oligosaccharide 0.05g；
Oligogalacturonans 0.1g；
Humic acid 20g；
Matrine 0.5g；
Nicotine 0.5g；
Sodium alginate: 50g；
Moisturizing is to 1000g
Other three groups of processing are respectively clear water control, asomate and the Wound-healing agent for not adding sodium alginate oligosaccharide.
120 sick scars are randomly selected in orchard to be tested, and lesion necrosis part is scraped off first, then smears four kinds of medicines
Agent is smeared 1 time.Then 60d, 180d and 270d count healing effect after treatment.
The experimental results are shown inthe following table:
Medicament sick scar is struck off after healing result (callus width mm)
The recurrence result of sodium alginate wound healing agent for plants prevention and treatment apple tree canker
The above results display is smeared sodium alginate oligosaccharide wound healing agent for plants only 1 time, to canker of apple fruit wound healing
Also there is apparent facilitation, handle callus width, be 3.56 times for commonly using 8 asomate of medicament processing width in production.
Simultaneously next year rot disease recurrence the result shows that, sodium alginate oligosaccharide wound healing agent for plants smear 1 time processing relative control effect is
90.00%, and asomate relative control effect is 60%, sodium alginate oligosaccharide is not added in as a result same display, the identical system of remaining ingredient
Agent also has good facilitation to the wound healing of canker of apple fruit.
Embodiment 3.
Sodium alginate oligosaccharide wound healing agent for plants is carried out in Shaanxi Xinping orchard to test the prevention and treatment of canker of apple fruit.
Formulation content formula are as follows:
Sodium alginate oligosaccharide 0.02g；
Oligogalacturonans 0.05g；
Humic acid 20g；
Nimbin 0.5g；
Jervine 0.5g；
Chitosan: 20g；
Moisturizing is to 1000g
Other four groups of processing are respectively clear water control, Guofukang, asomate and carbendazim.
100 sick scars are randomly selected in orchard to be tested, and are first scraped off lesion necrosis part, then in sick scar
Five kinds of medicaments of even smearing, and 1-2cm also coats consolidant outside away from sick scar, smears 2 times, interval time 15 days altogether.After 4 months
It investigates sick scar recurrence rate, control efficiency and measures sick scar healing width.Test result shows that sodium alginate oligosaccharide consolidant is handled
It does not recur, preventive effect is best, and averagely healing width reaches 15mm.
Healing effect of the 4. sodium alginate oligosaccharide wound healing agent for plants of embodiment to jujube tree band girdle wound
In Ankang, jujube garden has carried out the Healing Experiments that sodium alginate oligosaccharide consolidant promotes jujube tree band girdle wound.
Formulation content formula are as follows:
Sodium alginate oligosaccharide 0.01g；
Oligogalacturonans 0.075g；
Humic acid 30g；
Rotenone 0.5g；
Nicotine 0.5g；
Carragheen: 30g；
Moisturizing is to 1000g
Four groups of processing are respectively the control of I, clear water, II, sodium alginate oligosaccharide wound healing agent for plants band girdle pre-treatment, III,
Before the post-processing of sodium alginate oligosaccharide wound healing agent for plants band girdle and IV, sodium alginate oligosaccharide wound healing agent for plants band girdle
Reason+sodium alginate oligosaccharide wound healing agent for plants band girdle post-processing.
Test material is 4 years raw winter jujube treelets.It is divided into four groups by tree vigo(u)r random selection 120 to be tested, girdling period
For full-bloom stage, the sodium alginate oligosaccharide wound healing agent for plants band girdle pre-treatment time is to carry out smearing processing, band girdle within 1 day before band girdle
Width is 0.9cm.The post-processing of sodium alginate oligosaccharide wound healing agent for plants is carried out after band girdle again.Being cured for wound is detected after 30 days
Conjunction rate.And the fruit-setting rate of different disposal is detected after bearing fruit.
Function and effect of the sodium alginate wound healing agent for plants to jujube tree band girdle wound healing
The above results show that sodium alginate oligosaccharide wound healing agent for plants is handled before band girdle, also have to band girdle wound healing
Apparent facilitation, healing rate are significantly higher than clear water control.And hurt simultaneously using sodium alginate oligosaccharide plant before and after band girdle
Mouth consolidant has more obvious facilitation to the healing of band girdle wound, and healing rate is up to 93.3%.Simultaneously to band girdle after
The fruit-setting rate of jujube tree is also significantly improved effect.
Healing effect of the 5. sodium alginate oligosaccharide wound healing agent for plants of embodiment to jujube tree band girdle wound
In Hebei Xian County, jujube garden has carried out the Healing Experiments that sodium alginate oligosaccharide consolidant promotes jujube tree band girdle wound.
Formulation content formula are as follows:
Sodium alginate oligosaccharide 0.05g；
Oligogalacturonans 0.05g；
Humic acid 20g；
Jamaicin 0.5g；
Nicotine 1g；
Prolan polysaccharide: 30g；
Moisturizing is to 1000g
Four groups of processing are respectively the control of I, clear water, II, sodium alginate oligosaccharide wound healing agent for plants band girdle pre-treatment, III,
Before the post-processing of sodium alginate oligosaccharide wound healing agent for plants band girdle and IV, sodium alginate oligosaccharide wound healing agent for plants band girdle
Reason+sodium alginate oligosaccharide wound healing agent for plants band girdle post-processing.
Test material is golden jujube.It is divided into four groups by 200 plants of tree vigo(u)r random selection to be tested, girdling period is full blossom
Phase, the sodium alginate oligosaccharide wound healing agent for plants band girdle pre-treatment time, band girdle width was to carry out within 3 days smearing processing before band girdle
0.8cm.The post-processing of sodium alginate oligosaccharide wound healing agent for plants is carried out after band girdle again.The healing rate of wound is detected after 30 days.
And the fruit-setting rate of different disposal is detected after bearing fruit.
Function and effect of the sodium alginate wound healing agent for plants to jujube tree band girdle wound healing
Influence of the 6. sodium alginate oligosaccharide wound healing agent for plants of embodiment to grafting pear survival rate rate
Influence experiment of the sodium alginate oligosaccharide consolidant to pears graft survival rate has been carried out in Pucheng orchard.
Formulation content formula are as follows:
Sodium alginate oligosaccharide 0.1g；
Oligogalacturonans 0.05g；
Humic acid 25g；
Pyrethrin 0.5g；
Jervine 0.5g；
Sodium alginate: 30g；
Moisturizing is to 1000g
Above-mentioned pharmaceutical formulation is used for the grafting of 100 pear trees, grafting is carried out as scion using faint scent pears annotinous branch and is changed
It makes, wound smears this preparation after scion is grafted onto stock, wraps up wait survive；Routinely engrafting method is control.After 30 days with
Scion rudiment is standard, investigates cion survival rate；The survey showed that, and conventional graft survival rate 67% is transferred using this consolidant
It is connected into motility rate 96%.Scion growing state, scion average length 50.21cm after 1 year are investigated after 1 year, average branch diameter is
1.13cm, and scion average length 42.67cm is compareed, average branch diameter is 1.07cm average length.The above results display sea
Mosanom oligosaccharides wound healing agent for plants is obviously promoted effect to the healing of graft wound, can significantly improve graft
Survival rate, and compared with control, plant growing way is more vigorous after grafting.
Influence of the 7. sodium alginate oligosaccharide wound healing agent for plants of embodiment to grafting fruit tree survival rate rate
Influence experiment of the sodium alginate oligosaccharide consolidant to apple graft survival rate has been carried out in Shaanxi Ansai orchard.
Formulation content formula are as follows:
Sodium alginate oligosaccharide 0.01g；
Oligogalacturonans 0.05g；
Humic acid 10g；
Matrine 0.5g；
Nicotine 0.5g；
Sodium alginate: 20g；
Moisturizing is to 1000g
Above-mentioned pharmaceutical formulation is used for the grafting of 200 fruit trees, carries out grafting renovation by scion of Fuji apple's annotinous branch,
Wound smears this preparation after scion is grafted onto stock, wraps up wait survive；Routinely engrafting method is control.With scion after 30 days
Rudiment is standard, investigates cion survival rate；The survey showed that, and conventional graft survival rate 7% uses this consolidant grafting survival
Rate 97%.
8. sodium alginate oligosaccharide Wound-healing agent of embodiment promotes tree peony wound healing assay
It is real that influence of the sodium alginate oligosaccharide consolidant to tree peony wound healing has been carried out in Heze City, Shandong Province tree peony planting base
It tests.
Formulation content formula are as follows:
Sodium alginate oligosaccharide 0.01g；
Oligogalacturonans 0.05g；
Humic acid 10g；
Matrine 0.5g；
Nicotine 0.5g；
Chitosan: 30g；
Moisturizing is to 1000g
The plant for randomly selecting 20 normal growths artificially causes the wound of diameter 5mm in its root, is divided into 2 groups later,
Blank control group is without any processing, directly fills out back soil；Experimental group smears wound using sodium alginate oligosaccharide Wound-healing agent,
Then soil is filled out back.After two weeks, wound situation, control group wound healing rate 10%, experimental group wound healing rate 100% are checked.
The above results show that sodium alginate oligosaccharide wound healing agent for plants is also applied for the processing of flowering plants wound, can be obviously promoted
Wound healing.
Influence of 9. sodium alginate oligosaccharide of embodiment to IAA content in tobacco
Tobacco tissue culture suspension cell is taken, is filtered, cell is collected, by cell suspension cultures in the fluid nutrient medium newly prepared
In, and cell concentration is transferred to 1.5 × 106A ml-1, handled after stablizing 2 hours.
1ml cell is added in centrifuge tube, the sodium alginate oligosaccharide of various concentration is added by processing, respectively under dark conditions
After shaking table culture different time, cell sample is measured.
The measurement of IAA: cell sample 10000g is centrifuged 5min, in the horseradish peroxidase containing 0.025 μM and 0.1 μM
MTTA-Eu3+Centrifuged supernatant shading is added in the 0.05M pH4 acetate buffer liquid system of fluorescence probe and reacts 20min,
Fluorescence intensity is measured on 1420 fluorescence calculating instruments.
The sodium alginate oligosaccharide (0.1-100mg/kg) of various concentration handles the influence to IAA content in tobacco cell extremely
Significantly.The tobacco cell IAA content optimum concentration of sodium alginate oligosaccharide processing is 1.0mg/kg.From test result (Fig. 1),
Handle tobacco cell 8h after, intracellular IAA content it is highest be 1.0mg/kg, 0.12 μM.
Sodium alginate oligosaccharide (1mg/L) IAA content can increase in significant evoking tobacco cell.First 2h occurs after induction
One small peak, IAA content persistently increases later, and when 14h reaches maximum, is 0.35 μM.Hereafter again gradually decrease, for 24 hours when
Content is 0.06 μM (Fig. 2) close to control.
The above results show that sodium alginate oligosaccharide can induce the generation of plant endo auxin, and researches show that production China and foreign countries
Source plant hormone can also promote the healing of plants wound, but need strict control using concentration, when excessive concentration, can play
Side effect, and when concentration is lower, promote the effect of wound healing again unobvious.Main cause is exogenous growth element vulnerable to light point
Solution, into plant after again be easy by related enzyme hydrolysis, cause its promote wound healing effect it is unobvious.Therefore sodium alginate
Oligosaccharides may be accumulated by the plant endogenous auxin of Reasonable Regulation And Control, and then achieve the effect that promote wound healing, without producing
Crude drug evil, is one of the advantage that it is used as wound healing agent for plants.
Claims (5)
1. a kind of wound healing agent for plants containing sodium alginate oligosaccharide, it is characterised in that: consolidant is with sodium alginate oligosaccharide
Active ingredient, sodium alginate oligosaccharide average molecular weight is 500-5000Da, mass content 0.001%-0.02% in consolidant.
2. wound healing agent for plants described in accordance with the claim 1, it is characterised in that: in consolidant also containing plant induced resistance agent,
One of nutritional agents, fungicide, insecticide, film forming agent or two kinds or more.
3. wound healing agent for plants according to claim 2, it is characterised in that: contained plant induced resistance agent is specially oligomerization
Galacturonic acid, average molecular weight 500-3000Da, accounting for wound healing agent for plants weight percent is 0.005%-0.01%；Battalion
Feeding agent is specially humic acid, and accounting for wound healing agent for plants weight percent is 1%-5%；Fungicide be specially matrine, jamaicin,
One or more of pyrethrin, nimbin, rotenone, accounting for wound healing agent for plants weight percent is 0.05%-1%；It kills
Worm agent is specially one of jervine, nicotine or two kinds, and accounting for wound healing agent for plants weight percent is 0.05%-1%；At
Film is specially one of chitosan, sodium alginate, carragheen, prolan polysaccharide, Curdlan polysaccharide or two kinds or more, is accounted for
Wound healing agent for plants weight percent is 0.5%-5%.
4. according to wound healing agent for plants described in claim 1,2 or 3, it is characterised in that: the plant according to the actual situation
Ointment preparation or liquid preparation is made in object Wound-healing agent；
The liquid preparation is aqua, microemulsion, suspending agent or moisture content dispersing agent.
5. a kind of any one of claim 1-4 wound healing agent for plants is used to promote the purposes of plant wound heeling, special
Sign is: using on wound in 3 days after 1-3 days or vegetation pruning, grafting or band girdle before vegetation pruning, grafting or band girdle
The consolidant.
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|---|---|---|---|---|
| CN103651361A (en) * | 2012-03-07 | 2014-03-26 | 海南正业中农高科股份有限公司 | Plant wound protection liquid containing chitosan oligosaccharide |
| CN102617224B (en) * | 2012-03-07 | 2014-04-30 | 海南正业中农高科股份有限公司 | Plant wound protective agent containing chitosan oligosaccharide |
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2015
- 2015-12-02 CN CN201510868901.7A patent/CN106804605B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103651361A (en) * | 2012-03-07 | 2014-03-26 | 海南正业中农高科股份有限公司 | Plant wound protection liquid containing chitosan oligosaccharide |
| CN102617224B (en) * | 2012-03-07 | 2014-04-30 | 海南正业中农高科股份有限公司 | Plant wound protective agent containing chitosan oligosaccharide |
Non-Patent Citations (1)
| Title |
|---|
| 海藻酸钠寡糖生物活性的研究进展;张玉娟等;《中国酿造》;20140115(第01期);全文 * |
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