CN114766220A - Grafting seedling method for white magnolia - Google Patents
Grafting seedling method for white magnolia Download PDFInfo
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Abstract
The invention discloses a grafting and seedling raising method for magnolia denudata, which comprises the following steps: preparing grafting; selecting rootstocks; cutting the rootstock: cutting off a trunk at a position 10-20 cm away from the ground of the stock, longitudinally cutting the part with the section depth reaching the xylem by a cut with the depth of 2-3 cm, and then obliquely cutting the part with the depth of one half to two thirds of the longitudinal cutting by a cut with the angle of 30-45 degrees; selecting spike strips; scion cutting: cutting the scion into scions containing 1-2 buds, longitudinally cutting the back of the buds and the depth of the scions reaching the xylem for a time, wherein the longitudinal cutting depth is 2-3 cm, reversely beveling the scions at a position one third to one half of the distance from the base of the scions, and the beveling angle is 30-45 degrees; jointing; and (6) binding. The method well solves the problem of seedling production of the magnolia denudata by screening the suitable rootstocks and the suitable grafting technology.
Description
Technical Field
The invention relates to the field of plant grafting. More particularly, the invention relates to a grafting seedling method of magnolia denudata.
Background
The grafting propagation is to graft one part of plant nutritive organ onto other plant. The branch used for grafting is called scion, the bud used is called grafting bud, the grafted plant is called stock, and the grafted seedling is called grafted seedling. The principle of grafting survival is that the cambium of the joint between two plants with affinity generates a healing phenomenon, so that a conduit and a sieve tube are communicated with each other to form a new individual.
The magnolia denudata is a famous ornamental tree species, the stability of the excellent variety genetic character of the magnolia denudata can be maintained by grafting and seedling raising, but the survival rate is lower by adopting the existing grafting method.
Disclosure of Invention
The invention aims to provide a grafting and seedling raising method for magnolia denudata.
To achieve these objects and other advantages and in accordance with the purpose of the invention, as embodied and broadly described herein, there is provided a method for raising magnolia denudata seedlings by grafting, comprising:
preparing grafting: grafting is carried out in 9-4 months in the next year, the temperature is 20-30 ℃ during grafting, and rainy days are avoided;
selecting rootstocks: selecting 1-2 year old robust seedlings of the macyoya cordata with the ground diameter of 1.2-2 cm as rootstocks of the grafted seedlings of the magnolia denudata;
cutting the rootstock: cutting off a trunk at a position 10-20 cm away from the ground of the stock, longitudinally cutting a wood part at a position with a section depth of 2-3 cm, then obliquely cutting a wood part at a position with a half to two thirds of the longitudinal cutting depth at an oblique cutting angle of 30-45 degrees, taking off a cut part, and uniformly, straightly and smoothly cutting;
selecting spike strips: the scion selects a 1-year-old branch of a mother magnolia denudata tree;
scion cutting: cutting the scion into scions which contain 1-2 buds and are 4-10 cm long, longitudinally cutting the back of the buds and the depth of the scions to the xylem by one time, wherein the longitudinal cutting depth is 2-3 cm, reversely beveling the scions at the position one third to one half away from the base of the scions by one time, wherein the beveling angle is 30-45 degrees, and the cut parts are taken off and cut evenly, straightly and smoothly;
bonding: selecting a combination of a scion section and a stock section with the same size for jointing, aligning the scion section with the stock section during jointing, aligning the inclined plane of the scion with the inclined plane of the stock, aligning and jointing the cambium of the stock with the cambium of the scion, and aligning and jointing the cambium on one side of the scion section if the scion section is smaller than the stock section;
binding: and (3) the film belt is used for sealing and binding the part 2.0cm below the joint in a imbricated shape from bottom to top, the scion cannot move, and the binding is firm.
Preferably, in the grafting and seedling raising method of magnolia denudata, after the rootstock is cut, a disinfectant growth agent is coated on the section and the tangent plane, wherein the preparation method of the disinfectant growth agent comprises the following steps: adding 0.2-0.4 part by weight of cyclodextrin into 15-20 parts by weight of water, uniformly stirring, then adding 0.4-0.8 part by weight of curcumin derivative, 5-10 parts by weight of refined pyroligneous liquor 200 times and sodium alginate oligosaccharide, carrying out ultrasonic treatment for 20-30 min by using ultrasonic waves to prepare a first mixed solution, adding a plant growth regulator into the first mixed solution to prepare a second mixed solution, and then adding 3-5 parts by weight of carboxymethyl chitosan and 2-3 parts by weight of sodium alginate into the second mixed solution, and uniformly stirring; the mass fraction of the sodium alginate oligosaccharide in the disinfectant growth agent is 0.01-0.015%;
adding 0.2-0.4 part by weight of cyclodextrin into 15-20 parts by weight of water after scion cutting, uniformly stirring, then placing the section of the scion with the section, the section and the inclined plane into a cyclodextrin water solution for soaking for 10-20 min, then adding 0.4-0.8 part by weight of curcumin derivative, 5-10 parts by weight of refined pyroligneous liquor 200 times liquid and sodium alginate oligosaccharide, carrying out ultrasonic treatment for 20-30 min, then taking the scion upwards, suspending the scion above the previously soaked solution, enabling the liquid flowing down on the scion to completely flow into the previously soaked solution, then sequentially adding a plant growth regulator, 3-5 parts by weight of carboxymethyl chitosan and 2-3 parts by weight of sodium alginate, uniformly stirring to prepare a soaking solution, then placing the section of the scion with the section, the section and the inclined plane into the soaking solution for soaking for 5-10 min, and draining; the mass fraction of the sodium alginate oligosaccharide in the soaking solution is 0.01-0.015%.
Preferably, in the grafting and seedling raising method of magnolia denudata, the average molecular weight of the sodium alginate oligosaccharide is 500-5000 Da.
Preferably, in the grafting seedling method of magnolia denudata, the plant growth regulator comprises 6-BA, IBA and NAA, and the concentrations of the 6-BA, IBA and NAA in the disinfectant growth regulator and the soaking solution are 1.8-2.2 mg/L, 250-300 mg/L and 3-4 mg/L respectively.
Preferably, in the magnolia denudata grafting seedling method, the curcumin derivative is 1, 7-bis (p- (4-phenylbenzyloxy) phenyl) -1, 6-heptadiene-3, 5-dione.
Preferably, in the grafting seedling method of magnolia denudata, the addition amount of the curcumin derivative during preparation of the disinfectant growth agent and the soaking solution is 0.6 part by weight.
Preferably, in the magnolia denudata grafting seedling method, the cyclodextrin is gamma-cyclodextrin.
Preferably, in the grafting and seedling raising method of magnolia denudata, the concentrations of 6-BA, IBA and NAA in the disinfectant growth agent and the soaking solution are respectively 2mg/L, 270mg/L and 4 mg/L.
The invention at least comprises the following beneficial effects:
the method well solves the problem of seedling raising production of the magnolia denudata by screening suitable stocks and suitable grafting technologies.
According to the invention, after the rootstock is cut, the disinfection growth agent is smeared at the cut of the rootstock, and the scion is soaked in the soaking solution, so that the wound healing can be promoted, the cell growth of a wound part can be promoted, the growth and the propagation of microorganisms at the wound part can be inhibited, the evaporation of moisture at the wound part can be controlled, and the grafting survival rate can be improved.
According to the invention, sodium alginate oligosaccharide is introduced into the disinfection growth agent and the soak solution, and can promote the growth of cells at a wound part; wood vinegar is introduced, so that the wood vinegar has the effects of preventing bacteria and insects and providing nutrition for the healing of wound parts; carboxymethyl chitosan and sodium alginate are introduced, which can control the loss of moisture at the wound part and make other components attached to the wound part; the curcumin derivative is introduced, has good antibacterial effect, and can inhibit the growth and reproduction of microorganisms. The curcumin derivative is insoluble in water, the cyclodextrin can be dissolved in water, the curcumin derivative can be dissolved in a cyclodextrin water solution, the curcumin derivative can be indirectly dissolved in water by adding the cyclodextrin, and the active ingredients can be slowly released by embedding the active ingredients by the cyclodextrin, so that the curcumin derivative can play a role for a long time.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Detailed Description
The present invention is further described in detail below with reference to examples so that those skilled in the art can practice the invention with reference to the description.
It is to be noted that the experimental methods described in the following embodiments are all conventional methods unless otherwise specified, and the reagents and materials are commercially available unless otherwise specified.
Example 1
A grafting seedling method of white magnolia (white magnolia is a flower of white magnolia (Michelia alba) of Michelia of Magnoliaceae, which is called white magnolia in folk practice and is a fragrant flower plant of Guangxi specialty) comprises the following steps:
grafting preparation: grafting in 9 months at 20 ℃ to avoid rainy days;
selecting the rootstock: selecting a 1-year-old healthy seedling of a fire nan (Michelia macclurei Dandy) with the ground diameter of 1.2cm as a stock of a magnolia denudata grafted seedling;
cutting the rootstock: cutting off a trunk at a position 10cm away from the ground of the stock, longitudinally cutting a wood part at a position with a section depth of 2cm, then obliquely cutting a half part of the longitudinal cutting depth at an oblique cutting angle of 30 degrees, taking off a cut part, and uniformly and smoothly cutting;
selecting spike strips: the scion selects a 1-year-old branch of a mother magnolia denudata tree;
scion cutting: cutting the scion into scions which contain 1 bud and are 4cm long, longitudinally cutting the back of the bud and the part which is as deep as the xylem by a depth of 2cm, reversely beveling the part which is one third of the scion base by a bevel angle of 30 degrees, taking off the cut part, and uniformly, straightly and smoothly cutting;
bonding: selecting a combination of a scion section and a stock section with the same size for jointing, aligning the scion section with the stock section during jointing, aligning the inclined plane of the scion with the inclined plane of the stock, aligning and jointing the cambium of the stock with the cambium of the scion, and aligning and jointing the cambium on one side of the scion section if the scion section is smaller than the stock section;
binding: and (3) the film belt is used for sealing and binding the part 2.0cm below the joint in a imbricated shape from bottom to top, the scion cannot move, and the binding is firm.
The production of the magnolia denudata is not easy to fruit, and the production problem of magnolia denudata seedling culture is well solved by screening a suitable stock and a suitable grafting technology.
In the grafting seedling method of the magnolia denudata, after the rootstock is cut, a disinfection growth agent is coated on the section and the tangent plane, wherein the preparation method of the disinfection growth agent comprises the following steps: adding 0.2 part by weight of cyclodextrin into 15 parts by weight of water, uniformly stirring, then adding 0.4 part by weight of curcumin derivative, 5 parts by weight of refined pyroligneous liquor 200 times and sodium alginate oligosaccharide, performing ultrasonic treatment for 20min by using ultrasonic waves to prepare a first mixed solution, adding a plant growth regulator into the first mixed solution to prepare a second mixed solution, then adding 3 parts by weight of carboxymethyl chitosan and 2 parts by weight of sodium alginate into the second mixed solution, and uniformly stirring; the mass fraction of the sodium alginate oligosaccharide in the disinfectant growth agent is 0.01 percent; the refined pyroligneous liquor was prepared by the method of CN201410139741.8, a preparation method of a pyroligneous liquor water-soluble fertilizer in example 2.
Cutting the scion, adding 0.2 weight part of cyclodextrin into 15 weight parts of water, stirring uniformly, soaking the section of the scion with the section, the section and the inclined plane in a cyclodextrin water solution for 10min, adding 0.4 weight part of curcumin derivative, 5 weight parts of refined pyroligneous liquor 200 times liquid and sodium alginate oligosaccharide, performing ultrasonic treatment for 20min (ultrasonic treatment can fuse the curcumin derivative with the cyclodextrin and combine the curcumin derivative with the section of the scion), taking out the scion upwards, suspending the scion above the soaked solution, allowing the liquid flowing down on the scion to flow into the soaked solution completely, sequentially adding a plant growth regulator, 3 weight parts of carboxymethyl chitosan and 2 weight parts of sodium alginate, stirring uniformly to obtain a soaking solution, soaking the section of the scion with the section, the section and the inclined plane in the soaking solution for 5min, draining; the mass fraction of the sodium alginate oligosaccharide in the soak solution is 0.01 percent. The refined pyroligneous liquor was prepared by the method of CN201410139741.8, a preparation method of a pyroligneous liquor water-soluble fertilizer in example 2.
According to the invention, after the rootstock is cut, the disinfection growth agent is smeared at the cut of the rootstock, and the scion is soaked in the soaking solution, so that the wound healing can be promoted, the cell growth of a part of the wound can be promoted, the growth and the propagation of microorganisms at the wound part can be inhibited, the evaporation of water at the wound part can be controlled, the wound healing can be accelerated, and the grafting survival rate can be improved.
The sodium alginate oligosaccharide is introduced into the disinfection growth agent and the soak solution, and can promote the growth of cells at a wound part; wood vinegar is introduced, so that the wood vinegar has the effects of preventing bacteria and insects and providing nutrition for the healing of wound parts; carboxymethyl chitosan and sodium alginate are introduced, which can control the loss of moisture at the wound part and make other components attached to the wound part; the curcumin derivative is introduced, has good antibacterial effect, and can inhibit the growth and reproduction of microorganisms. The curcumin derivative is insoluble in water, the cyclodextrin can be dissolved in water, the curcumin derivative can be dissolved in a cyclodextrin water solution, the curcumin derivative can be indirectly dissolved in water by adding the cyclodextrin, and the active ingredients can be slowly released by embedding the active ingredients by the cyclodextrin, so that the curcumin derivative can play a role for a long time.
In the grafting and seedling raising method of the magnolia denudata, the average molecular weight of the sodium alginate oligosaccharide is 500-800 Da.
In the grafting seedling raising method of the magnolia denudata, the plant growth regulator comprises 6-BA, IBA and NAA, and the concentrations of the 6-BA, the IBA and the NAA in the disinfectant growth regulator and the soak solution are 1.8mg/L, 250mg/L and 3mg/L respectively.
In the grafting seedling method of magnolia denudata, the curcumin derivative is 1, 7-bis (p- (4-phenylbenzyloxy) phenyl) -1, 6-heptadiene-3, 5-diketone.
In the magnolia denudata grafting seedling method, the cyclodextrin is gamma-cyclodextrin.
Example 2
A grafting seedling method of white magnolia (white magnolia is a flower of white magnolia (Michelia alba) of Michelia of Magnoliaceae, which is called white magnolia in folk practice and is a fragrant flower plant of Guangxi specialty) comprises the following steps:
grafting preparation: grafting in 10 months at 25 deg.c to avoid rainy days;
selecting rootstocks: selecting a 1.5-year-old healthy seedling of a fire nan (Michelia macclurei Dandy) with the ground diameter of 1.6cm as a stock of a magnolia alba grafted seedling;
cutting the rootstock: cutting off the trunk at a position 15cm away from the ground of the stock, longitudinally cutting the position with the section depth reaching the xylem by a depth of 2.5cm, then obliquely cutting the position with the longitudinal cutting depth of two thirds by a cut angle of 37 degrees, taking off the cut part, and cutting uniformly, straightly and smoothly;
selecting spike strips: the scion is a 1-year-old branch of a mother magnolia denudata tree;
scion cutting: cutting the scion into scions which contain 1 bud and are 7cm long, longitudinally cutting the back of the bud and the part which is as deep as the xylem by a knife with the depth of 2.5cm, reversely beveling the part which is one third away from the base part of the scion by a knife with the beveling angle of 37 degrees, taking off the cut part, and uniformly, straightly and smoothly cutting;
bonding: selecting a combination with the scion section and the stock section with the same size for jointing, aligning the scion section with the stock section during jointing, aligning the scion inclined plane with the stock inclined plane, aligning and jointing the stock cambium with the scion cambium, and aligning and jointing the cambium on one side if the scion section is smaller than the stock section;
binding: and (3) the film belt is used for sealing and binding the part 2.0cm below the joint in a imbricated shape from bottom to top, the scion cannot move, and the binding is firm.
The production of the white magnolia is not easy to fruit, and the production problem of the white magnolia seedling is well solved by screening a suitable stock and a suitable grafting technology.
In the grafting seedling method of the magnolia denudata, after stock is cut, a disinfecting growth agent is coated on the section and the tangent plane, wherein the preparation method of the disinfecting growth agent comprises the following steps: adding 0.3 part by weight of cyclodextrin into 17 parts by weight of water, uniformly stirring, then adding 0.6 part by weight of curcumin derivative, 7 parts by weight of refined pyroligneous liquor 200 times liquid and sodium alginate oligosaccharide, carrying out ultrasonic treatment for 25min by using ultrasonic waves to prepare a first mixed solution, adding a plant growth regulator into the first mixed solution to prepare a second mixed solution, and then adding 4 parts by weight of carboxymethyl chitosan and 2.5 parts by weight of sodium alginate into the second mixed solution, and uniformly stirring; the mass fraction of the sodium alginate oligosaccharide in the disinfectant growth agent is 0.013%; the refined pyroligneous liquor was prepared by the method of CN201410139741.8, a preparation method of a pyroligneous liquor water-soluble fertilizer in example 2.
Cutting the scion, adding 0.3 weight part of cyclodextrin into 17 weight parts of water, stirring uniformly, soaking the section of the scion with the section, the section and the inclined plane in a cyclodextrin water solution for 15min, adding 0.6 weight part of curcumin derivative, 7 weight parts of refined pyroligneous liquor 200 times liquid and sodium alginate oligosaccharide, performing ultrasonic treatment for 25min (ultrasonic treatment can fuse the curcumin derivative with the cyclodextrin and combine the curcumin derivative with the section of the scion), taking out the scion upwards, suspending the scion above the soaked solution to allow the liquid flowing down on the scion to flow into the soaked solution, sequentially adding a plant growth regulator, 4 weight parts of carboxymethyl chitosan and 2.5 weight parts of sodium alginate, stirring uniformly to obtain a soaking solution, soaking the section of the scion with the section, the section and the inclined plane in the soaking solution for 8min, draining; the mass fraction of the sodium alginate oligosaccharide in the soaking solution is 0.013%. The refined pyroligneous liquor was prepared by the method of CN201410139741.8, a preparation method of a pyroligneous liquor water-soluble fertilizer in example 2.
According to the invention, after the rootstock is cut, the disinfection growth agent is smeared at the cut of the rootstock, and the scion is soaked in the soaking solution, so that the wound healing can be promoted, the cell growth of a part of the wound can be promoted, the growth and the propagation of microorganisms at the wound part can be inhibited, the evaporation of water at the wound part can be controlled, the wound healing can be accelerated, and the grafting survival rate can be improved.
The sodium alginate oligosaccharide is introduced into the disinfection growth agent and the soak solution, and can promote the growth of cells at a wound part; wood vinegar is introduced, so that the wood vinegar has the effects of preventing bacteria and insects and providing nutrition for the healing of wound parts; carboxymethyl chitosan and sodium alginate are introduced, which can control the loss of moisture at the wound part and make other components attached to the wound part; the curcumin derivative is introduced, has good antibacterial effect, and can inhibit the growth and reproduction of microorganisms. The curcumin derivative is insoluble in water, the cyclodextrin can be dissolved in water, the curcumin derivative can be dissolved in a cyclodextrin water solution, the curcumin derivative can be indirectly dissolved in water by adding the cyclodextrin, and the active ingredients can be slowly released by embedding the active ingredients by the cyclodextrin, so that the curcumin derivative can play a role for a long time.
In the magnolia denudata grafting seedling method, the average molecular weight of the sodium alginate oligosaccharide is 2000-3000 Da.
In the grafting and seedling raising method of the magnolia denudata, the plant growth regulator comprises 6-BA, IBA and NAA.
In the magnolia denudata grafting seedling method, the curcumin derivative is 1, 7-bis (p- (4-phenylbenzyloxy) phenyl) -1, 6-heptadiene-3, 5-dione.
In the magnolia denudata grafting seedling method, the cyclodextrin is gamma-cyclodextrin.
In the grafting and seedling raising method of the magnolia denudata, the concentrations of 6-BA, IBA and NAA in the disinfectant growth agent and the soak solution are respectively 2mg/L, 270mg/L and 4 mg/L.
Example 3
A grafting seedling method of white magnolia (white magnolia is a flower of white magnolia (Michelia alba) of Michelia of Magnoliaceae, which is called white magnolia in folk practice and is a fragrant flower plant of Guangxi specialty) comprises the following steps:
preparing grafting: grafting is carried out in 4 months, the temperature during grafting is 30 ℃, and the rainy weather is avoided;
selecting rootstocks: selecting a 2-year-old healthy seedling of a machilus (Michelia macclurei Dandy) with the ground diameter of 2cm as a stock of a magnolia denudata grafted seedling;
cutting the rootstock: cutting off a trunk at a position 20cm away from the ground of the stock, longitudinally cutting a wood part at a section depth of 3cm, then obliquely cutting a wood part at two thirds of the longitudinal cutting depth at an oblique cutting angle of 45 degrees, taking off a cut part, and uniformly and smoothly cutting;
selecting spike strips: the scion selects a 1-year-old branch of a mother magnolia denudata tree;
scion cutting: cutting the scion into scions which contain 2 buds and are 10cm long, longitudinally cutting the back of the buds and the depth of the scions to a depth of xylem for a time, wherein the longitudinal cutting depth is 3cm, then reversely beveling the half of the scion base for a time, wherein the beveling angle is 45 degrees, and taking off the cut parts to cut the scions evenly and smoothly;
bonding: selecting a combination with the scion section and the stock section with the same size for jointing, aligning the scion section with the stock section during jointing, aligning the scion inclined plane with the stock inclined plane, aligning and jointing the stock cambium with the scion cambium, and aligning and jointing the cambium on one side if the scion section is smaller than the stock section;
binding: and (3) the film belt is used for sealing and binding the scion from bottom to top at a position 2.0cm below the joint, the scion cannot be shifted, and the binding is firm.
The production of the magnolia denudata is not easy to fruit, and the production problem of magnolia denudata seedling culture is well solved by screening a suitable stock and a suitable grafting technology.
In the grafting seedling method of the magnolia denudata, after the rootstock is cut, a disinfection growth agent is coated on the section and the tangent plane, wherein the preparation method of the disinfection growth agent comprises the following steps: adding 0.4 part by weight of cyclodextrin into 20 parts by weight of water, uniformly stirring, then adding 0.8 part by weight of curcumin derivative, 10 parts by weight of refined pyroligneous liquor 200 times liquid and sodium alginate oligosaccharide, carrying out ultrasonic treatment for 30min by using ultrasonic waves to prepare a first mixed solution, adding a plant growth regulator into the first mixed solution to prepare a second mixed solution, adding 5 parts by weight of carboxymethyl chitosan and 3 parts by weight of sodium alginate into the second mixed solution, and uniformly stirring; the mass fraction of the sodium alginate oligosaccharide in the disinfectant growth agent is 0.015 percent; the refined pyroligneous liquor was prepared by the method of CN201410139741.8, a preparation method of a pyroligneous liquor water-soluble fertilizer in example 2.
After cutting the scion, adding 0.4 weight part of cyclodextrin into 20 weight parts of water, uniformly stirring, then placing the section of the scion with the section, the section and the inclined plane into a cyclodextrin water solution for soaking for 20min, then adding 0.8 weight part of curcumin derivative, 10 weight parts of refined pyroligneous liquid 200 times liquid and sodium alginate oligosaccharide, then using ultrasonic wave to carry out ultrasonic treatment for 30min (the curcumin derivative and the cyclodextrin are fused by ultrasonic wave ultrasonic treatment, and the curcumin derivative is combined to the section of the scion), then taking out the scion upwards and suspending the scion above the previously soaked solution, leading the liquid flowing down on the scion to completely flow into the previously soaked solution, then sequentially adding a plant growth regulator, 5 weight parts of carboxymethyl chitosan and 3 weight parts of sodium alginate, uniformly stirring to prepare a soaking solution, then placing the section of the scion with the section, the section and the inclined plane into the soaking solution for soaking for 10min, draining; the mass fraction of the sodium alginate oligosaccharide in the soaking solution is 0.015 percent. The refined wood vinegar was prepared by the method of CN201410139741.8 a preparation method of wood vinegar water-soluble fertilizer in example 2.
According to the invention, after the rootstock is cut, the disinfection growth agent is smeared at the cut of the rootstock, and the scion is soaked in the soaking solution, so that the wound healing can be promoted, the cell growth of a part of the wound can be promoted, the growth and the propagation of microorganisms at the wound part can be inhibited, the evaporation of water at the wound part can be controlled, the wound healing can be accelerated, and the grafting survival rate can be improved.
According to the invention, sodium alginate oligosaccharide is introduced into the disinfection growth agent and the soak solution, and can promote the growth of cells at a wound part; wood vinegar is introduced, which has the effects of preventing bacteria and insects and providing nutrition for the healing of wound parts; carboxymethyl chitosan and sodium alginate are introduced, which can control the loss of moisture at the wound part and make other components attached to the wound part; the curcumin derivative is introduced, has good antibacterial effect, and can inhibit the growth and reproduction of microorganisms. The curcumin derivative is insoluble in water, the cyclodextrin can be dissolved in water, the curcumin derivative can be dissolved in a cyclodextrin water solution, the cyclodextrin is added to indirectly dissolve the curcumin derivative in water, and the cyclodextrin is used for embedding the active ingredients to slowly release the active ingredients, so that the curcumin derivative can play a role for a long time.
In the grafting and seedling raising method of the magnolia denudata, the average molecular weight of the sodium alginate oligosaccharide is 4000-5000 Da.
In the magnolia denudata grafting seedling method, the plant growth regulator comprises 6-BA, IBA and NAA, and the concentrations of the 6-BA, the IBA and the NAA in the disinfection growth regulator and the soak solution are respectively 2.2mg/L, 300mg/L and 4 mg/L.
In the magnolia denudata grafting seedling method, the curcumin derivative is 1, 7-bis (p- (4-phenylbenzyloxy) phenyl) -1, 6-heptadiene-3, 5-dione.
In the magnolia denudata grafting seedling method, the cyclodextrin is gamma-cyclodextrin.
Example 4
A grafting seedling method of white magnolia (white magnolia is a flower of white magnolia (Michelia alba) of Michelia of Magnoliaceae, which is called white magnolia in folk practice and is a fragrant flower plant of Guangxi specialty) comprises the following steps:
preparing grafting: grafting is carried out in 10 months, the temperature is 25 ℃ during grafting, and rainy days are avoided;
selecting the rootstock: selecting a 1.5-year-old healthy seedling of a Michelia macclurei (Michelia macclurei Dandy) with the ground diameter of 1.6cm as a stock of a magnolia denudata grafted seedling;
cutting the rootstock: cutting off a trunk at a position 15cm away from the ground of the stock, longitudinally cutting a wood part at a section depth of 2.5cm, then obliquely cutting a cut at two thirds of the longitudinal cutting depth at an oblique cutting angle of 37 degrees, taking off a cut part, and uniformly and smoothly cutting;
selecting spike strips: the scion is a 1-year-old branch of a mother magnolia denudata tree;
scion cutting: cutting the scion into scions which contain 1 bud and are 7cm long, longitudinally cutting the back of the bud and the part as deep as the xylem by a knife with the longitudinal cutting depth of 2.5cm, reversely beveling the part one third away from the scion base by a knife with the beveling angle of 37 degrees, taking off the cut parts, and uniformly and smoothly cutting;
bonding: selecting a combination of a scion section and a stock section with the same size for jointing, aligning the scion section with the stock section during jointing, aligning the inclined plane of the scion with the inclined plane of the stock, aligning and jointing the cambium of the stock with the cambium of the scion, and aligning and jointing the cambium on one side of the scion section if the scion section is smaller than the stock section;
binding: and (3) the film belt is used for sealing and binding the scion from bottom to top at a position 2.0cm below the joint, the scion cannot be shifted, and the binding is firm.
The production of the magnolia denudata is not easy to fruit, and the production problem of magnolia denudata seedling culture is well solved by screening a suitable stock and a suitable grafting technology.
Comparative example 1
Different from the embodiment 2, the curcumin is introduced into the growth-disinfecting agent and the soaking solution, namely the curcumin is used for replacing a curcumin derivative, and the rest is the same as the embodiment 2.
Comparative example 2
The difference from the example 2 is that the addition amount of the curcumin derivative in the preparation of the disinfectant growth agent and the soak solution is 0.5 part by weight, and the other steps are the same as the example 2.
Comparative example 3
The difference from the example 2 is that the addition amount of curcumin derivative in the preparation of the disinfectant growth agent and the soak solution is 0.7 parts by weight, and the other is the same as the example 2.
Comparative example 4
Unlike example 2, water was used in place of cyclodextrin and curcumin derivative, and the other was the same as example 2.
Comparative example 5
The difference from example 4 is that the rootstock is yellow blue, and the rest is the same as example 4.
Comparative example 6
The rootstock was michelia as in example 4, except that it was the same as in example 4.
Experiment 1
100 plants were grafted by the methods of example 2 and comparative examples 1 to 4, respectively, and after 30 days of grafting, the survival of the scions was investigated using scion germination as a standard, the survival rate was calculated, and the average length of the scions and the average diameter of the shoots were investigated after 1 year, and the results are shown in table 1.
TABLE 1 scion survival rate, average Length and mean shoot diameter
| Item | Survival rate/% | Average length/cm of scion | Average diameter/cm of branches |
| Example 2 | 98 | 61.25 | 1.89 |
| Comparative example 1 | 87 | 54.32 | 1.52 |
| Comparative example 2 | 91 | 57.91 | 1.63 |
| Comparative example 3 | 98 | 61.51 | 1.91 |
| Comparative example 4 | 75 | 49.63 | 1.29 |
Comparing example 2 with comparative example 1, it can be seen that, in example 2, by introducing a curcumin derivative having a better antibacterial effect than curcumin, growth and propagation of microorganisms can be better inhibited after grafting, so that a good growth environment can be provided for the growth of scions, the survival rate of scions can be further improved, the average length of the scions is longer, and the branches are thicker.
Comparing example 2 with comparative example 2, it is understood that when the added amount of curcumin derivative is decreased, the scion survival rate is decreased, the average length of the scion is shortened, and the shoot is thinned, and comparing example 2 with comparative example 3, although the added amount of curcumin derivative is increased, the survival rate is not increased, and the average length of the scion and the average diameter of the shoot do not change much, so from the viewpoint of cost, the optimum added amount of curcumin derivative in preparing the sterilized growth medium and the soaking solution is 0.6 parts by weight.
Comparing example 2 with comparative example 4, it can be seen that the survival rate of the scions can be greatly improved, the average length of the scions is longer, and the branches are thicker by introducing curcumin derivatives with better antibacterial effect in example 2.
Experiment 2
Grafting was performed according to the method of example 4, comparative examples 5 and 6, 20 grafts were planted per mu, the grafts were dwarfed from the second year, the plant height was maintained at a height convenient for harvesting, the graft survival rate (%), the half-year plant height (cm), the 1-year plant height (cm), the 2-year breast diameter (cm), the 3-year breast diameter (cm), the 5-year breast diameter (cm), the 3-year flower yield (kg/plant) and the 5-year flower yield (kg/plant) were determined, and the average value was taken, and the results were shown in table 2. Wherein the diameter at breast height is the diameter of a trunk at the position of 1m of plant height; the flower yield is the annual flower yield of each plant. The maximum flower yield of the grafted seedlings grafted by the grafting method in the embodiment 5 can be reached in the fifth year, and the flower yield is kept stable.
As can be seen from Table 2, when the Machilus thunbergii (Michelia javanica) is used as the rootstock, the survival rate after grafting is higher, and the flower yield in 3 years and the flower yield in 5 years are also higher.
While embodiments of the invention have been described above, it is not intended to be limited to the details shown, particular embodiments, but rather to those skilled in the art, and it is to be understood that the invention is capable of numerous modifications and that various changes may be made therein without departing from the spirit and scope of the invention as defined by the appended claims and their equivalents.
Claims (8)
1. The grafting seedling method of the magnolia denudata is characterized by comprising the following steps:
preparing grafting: grafting is carried out in 9-4 months next year, the temperature during grafting is 20-30 ℃, and rainy days are avoided;
selecting rootstocks: selecting 1-2 year old robust seedlings of the fire nanmu with the ground diameter of 1.2-2 cm as rootstocks of the grafting seedlings of the magnolia denudata;
cutting the rootstock: cutting off a trunk at a position 10-20 cm away from the ground of the stock, longitudinally cutting a wood part at a position with a section depth of 2-3 cm, then obliquely cutting a wood part at a position with a half to two thirds of the longitudinal cutting depth at an oblique cutting angle of 30-45 degrees, taking off a cut part, and uniformly, straightly and smoothly cutting;
selecting spike strips: the scion selects a 1-year-old branch of a mother magnolia denudata tree;
scion cutting: cutting the scion into scions which contain 1-2 buds and are 4-10 cm long, longitudinally cutting the back of the buds and the depth of the scions to a xylem part for a time, longitudinally cutting the scions for a depth of 2-3 cm, reversely beveling the scions at a position one third to one half of the distance from the scion base part for a time, beveling the scions at an angle of 30-45 degrees, taking off cut parts, and cutting the scions to be even, straight and smooth;
bonding: selecting a combination with the scion section and the stock section with the same size for jointing, aligning the scion section with the stock section during jointing, aligning the scion inclined plane with the stock inclined plane, aligning and jointing the stock cambium with the scion cambium, and aligning and jointing the cambium on one side if the scion section is smaller than the stock section;
binding: and (3) the film belt is used for sealing and binding the scion from bottom to top at a position 2.0cm below the joint, the scion cannot be shifted, and the binding is firm.
2. The grafting seedling method of magnolia denudata according to claim 1, wherein after the rootstock is cut, a disinfectant growth agent is applied to the cross section and the tangent plane, wherein the disinfectant growth agent is prepared by the following steps: adding 0.2-0.4 part by weight of cyclodextrin into 15-20 parts by weight of water, uniformly stirring, then adding 0.4-0.8 part by weight of curcumin derivative, 5-10 parts by weight of refined pyroligneous liquor 200 times and sodium alginate oligosaccharide, carrying out ultrasonic treatment for 20-30 min by using ultrasonic waves to prepare a first mixed solution, adding a plant growth regulator into the first mixed solution to prepare a second mixed solution, and then adding 3-5 parts by weight of carboxymethyl chitosan and 2-3 parts by weight of sodium alginate into the second mixed solution, and uniformly stirring; the mass fraction of the sodium alginate oligosaccharide in the disinfectant growth agent is 0.01-0.015%;
after scion cutting, adding 0.2-0.4 part by weight of cyclodextrin into 15-20 parts by weight of water, uniformly stirring, then placing the section of the scion with the section, the section and the inclined plane into a cyclodextrin water solution, soaking for 10-20 min, then adding 0.4-0.8 part by weight of curcumin derivative, 5-10 parts by weight of refined pyroligneous liquor 200 times and sodium alginate oligosaccharide, ultrasonically treating for 20-30 min by ultrasonic waves, then taking the scion upwards, suspending the scion above the previously soaked solution, enabling all liquid flowing down on the scion to flow into the previously soaked solution, then sequentially adding a plant growth regulator, 3-5 parts by weight of carboxymethyl chitosan and 2-3 parts by weight of sodium alginate, uniformly stirring to prepare a soaking solution, then placing the section of the scion with the section, the section and the inclined plane into the soaking solution, soaking for 5-10 min, and draining; the mass fraction of the sodium alginate oligosaccharide in the soaking solution is 0.01-0.015%.
3. The magnolia denudata grafting seedling method as set forth in claim 2, wherein the average molecular weight of the sodium alginate oligosaccharide is 500-5000 Da.
4. The grafting seedling raising method of magnolia denudata according to claim 2, wherein the plant growth regulator comprises 6-BA, IBA and NAA, and the concentrations of the 6-BA, IBA and NAA in the disinfection growth regulator and the soaking solution are 1.8-2.2 mg/L, 250-300 mg/L and 3-4 mg/L respectively.
5. The grafting seedling raising method of magnolia denudata as claimed in claim 2, wherein the curcumin derivative is 1, 7-bis (p- (4-phenylbenzyloxy) phenyl) -1, 6-heptadiene-3, 5-dione.
6. The grafting seedling method of magnolia denudata as claimed in claim 5, wherein the curcumin derivative is added in an amount of 0.6 parts by weight when the disinfectant growth agent and the soak solution are prepared.
7. The grafting seedling method of magnolia denudata as claimed in claim 2, wherein the cyclodextrin is γ -cyclodextrin.
8. The grafting seedling raising method of magnolia denudata as claimed in claim 4, wherein the concentrations of 6-BA, IBA and NAA in the disinfectant growth agent and the soaking solution are 2mg/L, 270mg/L and 4mg/L respectively.
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