CN107750695A - A kind of engrafting method of Ponkan - Google Patents
A kind of engrafting method of Ponkan Download PDFInfo
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- CN107750695A CN107750695A CN201711182293.XA CN201711182293A CN107750695A CN 107750695 A CN107750695 A CN 107750695A CN 201711182293 A CN201711182293 A CN 201711182293A CN 107750695 A CN107750695 A CN 107750695A
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Abstract
The invention discloses a kind of engrafting method of Ponkan, comprise the following steps：(1) brick wood test tube is sowed：Full Ponkan seed is seeded in blake bottle, blake bottle is placed on culturing rack after culture to seed sprouting, then be placed under illumination and be trained rootstock seedling；(2) a species of orchid detoxification treatment is connect：Tender Ponkan bud is chosen as twig, twig is cut into stem apex, stem apex is placed in disinfectant solution after cleaning, and uses Co60Gamma-ray irradiation completes detoxification treatment；3) grafting：Rootstock seedling is migrated in soil, after rootstock seedling germination, a species of orchid will be connect and grafted on rootstock seedling；(4) managing after grafting：Wound is smeared using Wound-healing agent after grafting, then using infrared light and incandescent light alternately irradiated Ponkan grafting.The present invention uses Ponkan gemmule graft technology, and docking a species of orchid carries out detoxification, and obtained Ponkan seedling is virus-free, not with yellow twig, bark cracking, decline disease, broken leaf disease, grows, high graft survival rate.
Description
Technical field
The present invention relates to planting fruit trees industry technical field, and in particular to a kind of engrafting method of Ponkan.
Background technology
Ponkan is typically born in hillside woods side, or in bushes, Ponkan strong adaptability, happiness is nice and cool, the environment of moistening, i.e., cold-resistant
High temperature resistant again, can grow between -36~43 DEG C.Light be also resistant to it is shady, be typically distributed about bare hills and mountains, tailo, half tailo,
Mountain valley, the gradient is preferably 15~25 degree.It is drought-enduring, during excess moisture, the easy excessive growth of branches and leaves.Not strict is required to soil, but in soil
Layer depth is thick, fertile, loose, the well-drained subacidity sandy loam well-grown of quality.
Ponkan causes the one or more viruses of Ponkan easy infection by long-term asexual grafting, causes tree vigo(u)r to fail, yield
Decline, fruit quality is deteriorated, and research shows, the caused disease of Ponkan virus and viroid mainly has Citrus Huanglongbing pathogen, citrus
Broken leaf disease, citrus decline, exocortis.During Ponkan grafting, detoxification treatment is carried out to grafting a species of orchid, the disease-resistant of Ponkan can be improved
Property.
The content of the invention
Instant invention overcomes in conventional genderless grafting, Ponkan, which contains virus and viroid, causes Ponkan growing way bad, yield
A kind of low technical problem, there is provided engrafting method of Ponkan.
To solve the above problems, the present invention adopts the following technical scheme that：
A kind of engrafting method of Ponkan comprises the following steps：
(1) brick wood test tube is sowed：Full Ponkan seed is seeded in blake bottle, by blake bottle be placed on culturing rack in
After culture is sprouted to seed under 23~25 DEG C of temperature conditionss, then it is placed under illumination and is trained rootstock seedling；In described blake bottle
Equipped with the culture medium being formulated as follows：In terms of mass parts, 8~10 parts of inositol, 2~3 parts of glycine, 2~6 parts of nicotinic acid, vitamin B12
~6 parts, 5~12 parts of vitamin B6,2~6 parts of manganese sulfate, 1~5 part of zinc sulfate, 3~6 parts of sodium permanganate, 1~5 part of copper sulphate,
2~5 parts of calcium chloride, 3~6 parts of potassium nitrate, 2~6 parts of ammonium nitrate, 1~3 part of potassium hydrogen phosphate, 5~7 parts of magnesium sulfate, sucrose 20~30
Part, 50~100 parts of agar, 500 parts of water；
(2) a species of orchid detoxification treatment is connect：Tender Ponkan bud is chosen as twig, twig is cut into 0.7~1mm stem apexs, stem apex
Clean after be placed in weight/mass percentage composition for ethanol 4~8%, sodium hypochlorite 5~8%, mercury chloride 1~3%, gibberellic acid 0.01%,
Horseshoe bark extract 0.1~1%, citrus bark extract 2~5%, tetracycline 4~5%, remaining is the disinfectant solution of water
In, and use Co602~4h of-gamma-ray irradiation completes detoxification treatment；
(3) grafting：Rootstock seedling is migrated in soil, after rootstock seedling germination, by the way of cut-grafting will connect a species of orchid transfers
It is connected on rootstock seedling；
(4) managing after grafting：Wound is smeared using Wound-healing agent after grafting, then handed over using infrared light and incandescent light
For irradiation Ponkan grafting, described infrared light and incandescent light alternating frequency is 1 time/5h.
Wherein, described Co60- gamma-ray irradiation dose is 8.2~9KGy.
Wherein, described Wound-healing agent is made up of the following raw material of mass parts：2~5 parts of titanium dioxide, Buffalo bur
22~35 parts of alcohol extract, 10~20 parts of climbing groundsel alcohol extract, 37~49 parts of Sievers wormwood alcohol extract, Tangut Anisodus Radix alcohol extract 19~26
Part, 1~3 part of PAA, 5~10 parts of SKGM.
Wherein, it is not less than 62.7mg/100g containing terpenoid in described citrus bark extract.
Wherein, described horseshoe bark extract extracts to obtain by the following method:Horseshoe skin crushed after being dried is crossed into 100 mesh
Sieve, the horseshoe skin after crushing is placed in supercritical extract axe, is 26 DEG C in extraction temperature using petroleum ether as entrainer, extraction
Pressure is 39MPa, and the flow velocity of carbon dioxide is that the isolated horseshoe bark extract after 10min is extracted under 20L/min.
Wherein, flavone compound is not less than 85.2mg/100g in described Corm Eleocharitis extract.
Wherein, the intensity of illumination of described infrared light is 2000~2419Lux, the intensity of illumination of described incandescent light
For 3000~3463Lux.
In the present invention, the flavonoid content that described Buffalo bur alcohol extracting contains is not less than 37mg/100g,
To a variety of germs by bacteriostasis.
In the present invention, in climbing groundsel alcohol extract, flavoxanthin content is not less than 158mg/100g, chrysanthemaxanthin senecionine
Content is not less than 23mg/100g, and senlciphylline content is not less than 47mg/100g
In the present invention, volatile oil content is not less than 83mg/100g in Sievers wormwood alcohol extract, and interior vinegar class and Azulene class material be not low
In 27mg/100g.
In the present invention, the content of alkaloid is not less than 92mg/100g in Tangut Anisodus Radix alcohol extract.
Described Buffalo bur alcohol extract, climbing groundsel alcohol extract, Sievers wormwood alcohol extract, Tangut Anisodus Radix alcohol extract are by plant
Obtained by supercritical carbon dioxide extracting.
The present invention has the advantages that compared with prior art：
(1) present invention uses Ponkan gemmule graft technology, and docking a species of orchid carries out detoxification, and obtained Ponkan seedling is virus-free, raw
Grow, Ponkan seedling is not with yellow twig, bark cracking, decline disease, broken leaf disease.
(2) present invention will connect a species of orchid be placed in weight/mass percentage composition for ethanol 4~8%, sodium hypochlorite 5~8%, mercury chloride 1~
3%th, gibberellic acid 0.01%, horseshoe bark extract 0.1~1%, citrus bark extract 2~5%, tetracycline 4~5%, remaining
For in the disinfectant solution of water, and coordinate Co60- gamma-ray irradiation, citrus bark extract promote to connect the flowing of a species of orchid tissue fluid,
Co60- gamma-ray irradiation can kill the virus connect in a species of orchid, outside viroid, can also improve tetracycline and sodium hypochlorite, the work of mercury chloride
Change energy, improve tetracycline and sodium hypochlorite, mercury chloride sterilization detoxification efficiency.The disinfectant of the present invention is thought mutually to coordinate, and raising connects a species of orchid
Detoxification efficiency.
(3) growth-promoting long component gibberellic acid, horseshoe bark extract, the extraction of citrus bark are contained in disinfectant solution of the invention
Thing, gibberellic acid are conventional auxin, and the terpenoid contained in horseshoe bark extract has preferable antibacterial work
With citrus bark extract contains the plant tissue liquid similar to Ponkan, will be used for that to be soaked in disinfectant molten for connecing the branch of a species of orchid
Liquid, plays a part of bacteria mildew-proof to branch wound, while growth accelerator solution composition is close to Ponkan tissue fluid composition, grafting
When be easy to the matter transportation of branch, improve the success rate of grafting, branch grafting time is short, and growth is fast.
(4) Wound-healing agent of the invention is by titanium dioxide, Buffalo bur alcohol extract, climbing groundsel alcohol extract, Sievers wormwood
Alcohol extract, Tangut Anisodus Radix alcohol extract, PAA, SKGM composition, titanium dioxide is photochemical catalyst, and the present invention adopts after grafting
It is that the incandescent light that 2000~2419Lux is infrared light and intensity of illumination is 3000~3463Lux replaces spoke with intensity of illumination
According to, on the one hand give the Ponkan of firm grafting suitable light energy, while the luminous energy used promotes Titanium Dioxide, to bacterium etc.
Pathogenic bacteria have inhibitory action, and PAA and SKGM are water-absorbing-retaining composition, Buffalo bur alcohol extract, climbing groundsel alcohol
Extract, Sievers wormwood alcohol extract, Tangut Anisodus Radix alcohol extract are the sterilising components of plant extracts, under illumination condition, titanium dioxide
The antibacterial factor of Buffalo bur alcohol extract, climbing groundsel alcohol extract, Sievers wormwood alcohol extract, Tangut Anisodus Radix alcohol extract can be excited, is improved
Buffalo bur alcohol extract, climbing groundsel alcohol extract, Sievers wormwood alcohol extract, Tangut Anisodus Radix alcohol extract antibacterial bacteriostatic ability, promote plant
The healing of wound.
Embodiment
With reference to embodiment and experiment, the invention will be further described.
Embodiment 1
A kind of engrafting method of Ponkan, comprises the following steps：
(1) brick wood test tube is sowed：Full Ponkan seed is seeded in blake bottle, by blake bottle be placed on culturing rack in
After culture is sprouted to seed under 25 DEG C of temperature conditionss, then it is placed under illumination and is trained rootstock seedling；It is equipped with described blake bottle
The culture medium being formulated as follows：In terms of mass parts, 8 parts of inositol, 3 parts of glycine, 2 parts of nicotinic acid, 6 parts of vitamin B1, vitamin B6 5
Part, 6 parts of manganese sulfate, 1 part of zinc sulfate, 6 parts of sodium permanganate, 1 part of copper sulphate, 5 parts of calcium chloride, 3 parts of potassium nitrate, 6 parts of ammonium nitrate, phosphorus
1 part of potassium hydrogen phthalate, 7 parts of magnesium sulfate, 20 parts of sucrose, 100 parts of agar, 500 parts of water；
(2) a species of orchid detoxification treatment is connect：Tender Ponkan bud is chosen as twig, twig is cut into 0.7mm stem apexs, stem apex is cleaned
After be placed in weight/mass percentage composition for ethanol 8%, sodium hypochlorite 5%, mercury chloride 3%, gibberellic acid 0.01%, horseshoe bark extract
0.1%, citrus bark extract 5%, tetracycline 4%, remaining is in the disinfectant solution of water, and uses irradiation dose as 9KGy
Co60- gamma-ray irradiation 2h completes detoxification treatment；It is not less than in the citrus bark extract containing terpenoid
62.7mg/100g；The horseshoe bark extract extracts to obtain by the following method:Horseshoe skin crushed after being dried is crossed into 100 mesh sieves, will
Horseshoe skin after crushing is placed in supercritical extract axe, is 26 DEG C in extraction temperature, extracting pressure is using petroleum ether as entrainer
39MPa, the flow velocity of carbon dioxide are that the isolated horseshoe bark extract after 10min is extracted under 20L/min；Described horseshoe
Flavone compound is not less than 85.2mg/100g in extract；
(3) grafting：Rootstock seedling is migrated in soil, after rootstock seedling germination, by the way of cut-grafting will connect a species of orchid transfers
It is connected on rootstock seedling；
(4) managing after grafting：Using 22 parts of 5 parts of titanium dioxide, the Buffalo bur alcohol extract by mass parts, thousand after grafting
In 20 parts of light alcohol extract, 37 parts of Sievers wormwood alcohol extract, 26 parts of Tangut Anisodus Radix alcohol extract, 1 part of PAA, 10 parts of SKGM composition
Wound-healing agent smear wound, then using infrared light and incandescent light alternately irradiated Ponkan grafting, described infrared lamp
Light and incandescent light alternating frequency are 1 time/5h；The intensity of illumination of described infrared light is 2000Lux, described incandescent light
Intensity of illumination be 3463Lux.
Embodiment 2
A kind of engrafting method of Ponkan, comprises the following steps：
(1) brick wood test tube is sowed：Full Ponkan seed is seeded in blake bottle, by blake bottle be placed on culturing rack in
After culture is sprouted to seed under 24 DEG C of temperature conditionss, then it is placed under illumination and is trained rootstock seedling；It is equipped with described blake bottle
The culture medium being formulated as follows：In terms of mass parts, 9 parts of inositol, 3 parts of glycine, 5 parts of nicotinic acid, pangamic acid part, vitamin B6 10
Part, 5 parts of manganese sulfate, 3 parts of zinc sulfate, 3 parts of sodium permanganate, 3 parts of copper sulphate, 3 parts of calcium chloride, 4 parts of potassium nitrate, 5 parts of ammonium nitrate, phosphorus
2 parts of potassium hydrogen phthalate, 6 parts of magnesium sulfate, 25 parts of sucrose, 80 parts of agar, 500 parts of water；
(2) a species of orchid detoxification treatment is connect：Tender Ponkan bud is chosen as twig, twig is cut into 0.8mm stem apexs, stem apex is cleaned
After be placed in weight/mass percentage composition for ethanol 5%, sodium hypochlorite 6%, mercury chloride 2%, gibberellic acid 0.01%, horseshoe bark extract
0.5%, citrus bark extract 3%, tetracycline 5%, remaining for water disinfectant solution in, and use irradiation dose for
8.5KGy Co60- gamma-ray irradiation 3h completes detoxification treatment；It is not less than in the citrus bark extract containing terpenoid
62.7mg/100g；The horseshoe bark extract extracts to obtain by the following method:Horseshoe skin crushed after being dried is crossed into 100 mesh sieves, will
Horseshoe skin after crushing is placed in supercritical extract axe, is 26 DEG C in extraction temperature, extracting pressure is using petroleum ether as entrainer
39MPa, the flow velocity of carbon dioxide are that the isolated horseshoe bark extract after 10min is extracted under 20L/min；Described horseshoe
Flavone compound is not less than 85.2mg/100g in extract；
(3) grafting：Rootstock seedling is migrated in soil, after rootstock seedling germination, by the way of cut-grafting will connect a species of orchid transfers
It is connected on rootstock seedling；
(4) managing after grafting：Using 30 parts of 3 parts of titanium dioxide, the Buffalo bur alcohol extract by mass parts, thousand after grafting
In 15 parts of light alcohol extract, 40 parts of Sievers wormwood alcohol extract, 20 parts of Tangut Anisodus Radix alcohol extract, 2 parts of PAA, 8 parts of SKGM composition
Wound-healing agent smear wound, then using infrared light and incandescent light alternately irradiated Ponkan grafting, described infrared lamp
Light and incandescent light alternating frequency are 1 time/5h；The intensity of illumination of described infrared light is 2364Lux, described incandescent light
Intensity of illumination be 3336Lux.
Embodiment 3
A kind of engrafting method of Ponkan, comprises the following steps：
(1) brick wood test tube is sowed：Full Ponkan seed is seeded in blake bottle, by blake bottle be placed on culturing rack in
After culture is sprouted to seed under 23 DEG C of temperature conditionss, then it is placed under illumination and is trained rootstock seedling；It is equipped with described blake bottle
The culture medium being formulated as follows：In terms of mass parts, 10 parts of inositol, 2 parts of glycine, 6 parts of nicotinic acid, vitamin B12 part, vitamin B6
12 parts, 2 parts of manganese sulfate, 5 parts of zinc sulfate, 3 parts of sodium permanganate, 5 parts of copper sulphate, 2 parts of calcium chloride, 6 parts of potassium nitrate, 2 parts of ammonium nitrate,
3 parts of potassium hydrogen phosphate, 5 parts of magnesium sulfate, 30 parts of sucrose, 50 parts of agar, 500 parts of water；
(2) a species of orchid detoxification treatment is connect：Tender Ponkan bud is chosen as twig, twig is cut into 1mm stem apexs, after stem apex is cleaned
Weight/mass percentage composition is placed in as ethanol 4%, sodium hypochlorite 8%, mercury chloride 1%, gibberellic acid 0.01%, horseshoe bark extract 1%,
Citrus bark extract 2%, tetracycline 5%, remaining is in the disinfectant solution of water, and uses irradiation dose as 8.2KGy's
Co60- gamma-ray irradiation 4h completes detoxification treatment；It is not less than 62.7mg/ containing terpenoid in the citrus bark extract
100g；The horseshoe bark extract extracts to obtain by the following method:Horseshoe skin crushed after being dried is crossed into 100 mesh sieves, after crushing
Horseshoe skin be placed in supercritical extract axe, using petroleum ether as entrainer, extraction temperature be 26 DEG C, extracting pressure 39MPa,
The flow velocity of carbon dioxide is that the isolated horseshoe bark extract after 10min is extracted under 20L/min；Described Corm Eleocharitis extract
Middle flavone compound is not less than 85.2mg/100g；
(3) grafting：Rootstock seedling is migrated in soil, after rootstock seedling germination, by the way of cut-grafting will connect a species of orchid transfers
It is connected on rootstock seedling；
(4) managing after grafting：Using 35 parts of 2 parts of titanium dioxide, the Buffalo bur alcohol extract by mass parts, thousand after grafting
In 10 parts of light alcohol extract, 49 parts of Sievers wormwood alcohol extract, 19 parts of Tangut Anisodus Radix alcohol extract, 3 parts of PAA, 5 parts of SKGM composition
Wound-healing agent smear wound, then using infrared light and incandescent light alternately irradiated Ponkan grafting, described infrared lamp
Light and incandescent light alternating frequency are 1 time/5h；The intensity of illumination of described infrared light is 2419Lux, described incandescent light
Intensity of illumination be 3000Lux.
Comparative example 1
The Ponkan engrafting method of comparative example 1 and embodiment 1 is essentially identical, and difference is, comparative example 1 does not use Co60- γ is penetrated
Line irradiation connects a species of orchid.
Comparative example 2
The Ponkan engrafting method of comparative example 2 and embodiment 1 is essentially identical, and difference is, comparative example 2 use irradiation dose for
2KGy Co60- gamma-ray irradiation connects a species of orchid.
Comparative example 3
The Ponkan engrafting method of comparative example 3 and embodiment 1 is essentially identical, and difference is, comparative example 3 does not use infrared light
With incandescent light alternately irradiated Ponkan grafting.
Comparative example 4
The Ponkan engrafting method of comparative example 4 and embodiment 1 is essentially identical, and difference is, comparative example 4 is using disinfectant by matter
Measure the following raw material composition of percentage composition：Ethanol 4%, sodium hypochlorite 8%, mercury chloride 1%, gibberellic acid 0.01%, tetracycline
5%, remaining is water.
Comparative example 5
The Ponkan engrafting method of comparative example 3 and embodiment 1 is essentially identical, and difference is, comparative example 3 use intensity of illumination for
3000Lux infrared light irradiance Ponkan grafting.
1~embodiment of embodiment 3 and the survival rate of the Ponkan grafting of 1~comparative example of comparative example 4 are recorded in table 1 below.
Table 1
| Detection | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | Comparative example 5 |
| Survival rate % | 98% | 97% | 97% | 63% | 75% | 83% | 90% | 73% |
The Ponkan sprigging that 1~embodiment of embodiment 3 and 1~comparative example of comparative example 4 are grafted is into ploughing, and two
Ponkan is counted after year with yellow twig, bark cracking, decline disease, illness rate (%) situation of broken leaf disease, is recorded in table 2 below.
Table 2
From in table 1, using the Co of suitable irradiation dose60- gamma-ray irradiation connects a species of orchid, using infrared light and incandescent lamp
Light alternately irradiated grafting, and a species of orchid is connect using suitable disinfectant processing can improve the survival rate of Ponkan grafting.
As shown in Table 2, the disease illness rate of embodiment 1 is 0, and 1~comparative example of comparative example 5 all suffers from different degrees of Huang
Imperial disease, bark cracking, decline disease, broken leaf disease.Illustrate the Co using suitable irradiation dose60- gamma-ray irradiation connects a species of orchid, and uses
The detoxification efficiency of docking a species of orchid is good after suitable infrared light and incandescent light the alternately irradiated grafting of suitable intensity of illumination, and Ponkan is not easy
It is ill.
Described above is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, the equal change completed or modification change under the technical spirit suggested by all present invention, all should belong to
Cover the scope of the claims in the present invention.
Claims (7)
1. a kind of engrafting method of Ponkan, it is characterised in that comprise the following steps：
(1) brick wood test tube is sowed：Full Ponkan seed is seeded in blake bottle, blake bottle is placed on culturing rack in 23~
After culture is sprouted to seed under 25 DEG C of temperature conditionss, then it is placed under illumination and is trained rootstock seedling；It is equipped with described blake bottle
The culture medium being formulated as follows：In terms of mass parts, 8~10 parts of inositol, 2~3 parts of glycine, 2~6 parts of nicotinic acid, vitamin B12~6
Part, 5~12 parts of vitamin B6,2~6 parts of manganese sulfate, 1~5 part of zinc sulfate, 3~6 parts of sodium permanganate, 1~5 part of copper sulphate, chlorine
Change 2~5 parts of calcium, 3~6 parts of potassium nitrate, 2~6 parts of ammonium nitrate, 1~3 part of potassium hydrogen phosphate, 5~7 parts of magnesium sulfate, sucrose 20~30
Part, 50~100 parts of agar, 500 parts of water；
(2) a species of orchid detoxification treatment is connect：Tender Ponkan bud is chosen as twig, twig is cut into 0.7~1mm stem apexs, stem apex is cleaned
After be placed in weight/mass percentage composition for ethanol 4~8%, sodium hypochlorite 5~8%, mercury chloride 1~3%, gibberellic acid 0.01%, horseshoe
Bark extract 0.1~1%, citrus bark extract 2~5%, tetracycline 4~5%, remaining for water disinfectant solution in, and
Using Co602~4h of-gamma-ray irradiation completes detoxification treatment；
(3) grafting：Rootstock seedling is migrated in soil, after rootstock seedling germination, by the way of cut-grafting will connect a species of orchid grafts in
On rootstock seedling；
(4) managing after grafting：Wound is smeared using Wound-healing agent after grafting, then using infrared light and incandescent light alternating spoke
According to Ponkan grafting, described infrared light and incandescent light alternating frequency is 1 time/5h.
A kind of 2. engrafting method of Ponkan according to claim 1, it is characterised in that described Co60- gamma-ray irradiation
Dosage is 8.2~9KGy.
3. the engrafting method of a kind of Ponkan according to claim 1, it is characterised in that described Wound-healing agent is by quality
The following raw material composition of part：2~5 parts of titanium dioxide, 22~35 parts of Buffalo bur alcohol extract, climbing groundsel alcohol extract 10~20
Part, 37~49 parts of Sievers wormwood alcohol extract, 19~26 parts of Tangut Anisodus Radix alcohol extract, 1~3 part of PAA, 5~10 parts of SKGM.
4. the engrafting method of a kind of Ponkan according to claim 1, it is characterised in that in described citrus bark extract
It is not less than 62.7mg/100g containing terpenoid.
5. the engrafting method of a kind of Ponkan according to claim 1, it is characterised in that described horseshoe bark extract is by such as
Lower method is extracted to obtain:Horseshoe skin crushed after being dried is crossed into 100 mesh sieves, the horseshoe skin after crushing is placed in supercritical extract axe
In, it is 26 DEG C, extracting pressure 39MPa in extraction temperature, the flow velocity of carbon dioxide is 20L/min using petroleum ether as entrainer
The isolated horseshoe bark extract after lower extraction 10min.
A kind of 6. engrafting method of Ponkan according to claim 1, it is characterised in that flavones in described Corm Eleocharitis extract
Class compound is not less than 85.2mg/100g.
7. the engrafting method of a kind of Ponkan according to claim 1, it is characterised in that the illumination of described infrared light is strong
Spend for 2000~2419Lux, the intensity of illumination of described incandescent light is 3000~3463Lux.
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| CN114391410A (en) * | 2022-01-19 | 2022-04-26 | 象山县农业经济特产技术推广中心 | Method for rapidly removing virus diseases of multi-embryo citrus varieties |
| CN114710991A (en) * | 2022-05-10 | 2022-07-08 | 浙江省柑橘研究所 | Method for culturing citrus seeds for grafting |
| CN114710991B (en) * | 2022-05-10 | 2023-03-10 | 浙江省柑橘研究所 | Method for culturing citrus seeds for grafting |
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