CN105993766A - Cultivation method for skiranui tangerine - Google Patents

Cultivation method for skiranui tangerine Download PDF

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CN105993766A
CN105993766A CN201610347073.7A CN201610347073A CN105993766A CN 105993766 A CN105993766 A CN 105993766A CN 201610347073 A CN201610347073 A CN 201610347073A CN 105993766 A CN105993766 A CN 105993766A
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fertilizer
fruit
seedling
weight
cultivation
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雷军强
韦颖婷
覃稳梅
李玉秋
莫晓君
韦倩梅
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XIANGZHOU COUNTY SCIENCE AND TECHNOLOGY BUREAU
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • A01C21/005Following a specific plan, e.g. pattern
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/30Grafting
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D1/00Fertilisers containing potassium
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F9/00Fertilisers from household or town refuse
    • C05F9/04Biological compost
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Soil Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Inorganic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention relates to a cultivation method for skiranui tangerine. The planting method comprises the following steps: seedling cultivation, soil selection, soil preparation, field planting, nutrient and water management, field management, and disease and pest control, wherein high-quality seeds of a Guangxi ponkan tree is selected and cultivated into a rootstock seedling with resistance; meanwhile, a stem tip of a Sichuan Pujiang skiranui tangerine variety is cultivated into an improved virus-free seedling by utilizing a stem-tip grafting technology; experimental plantation of the improved virus-free seedling succeeds in Guangxi; an inorganic fertilizer and an organic fertilizer are adopted for fertilization; and South Korea Enspray mineral oil is adopted for disease and pest control. The skiranui tangerine produced by using the planting method provided by the invention has the advantages of an average fruit weight of 200 grams or more, a maximum single fruit weight of 350 grams or more, a juice sugar degree of 14% or more, a maximum juice sugar degree of 18%, soft and juicy pulp, extremely-thin and soft capsule wall, crisp and sweet taste and excellent quality. By successful cultivation of the virus-free seedling, the occurrence of diseases and pests is reduced; the yield and the quality of skiranui tangerine are improved; the development of fruit planting industry in the mountainous region of Guangxi is promoted; and objective economic benefits for local farmers are created.

Description

A kind of implantation methods of ugly Citrus chachiensis Hort.
Technical field
The present invention relates to the implantation methods of economic plants, the implantation methods of the most ugly Citrus chachiensis Hort..
Background technology
Ugly Citrus chachiensis Hort., formal name used at school " does not knows fire ", also known as " convex top Citrus chachiensis Hort. ", " ugly Fructus Citri tangerinae ", " very ugly person " and " ugly orange ", is by Japanese the agriculture ministry Gardening test site in 1972 to see clearly and middle wild No. 3 Citrus chachiensis Hort. cross breedings.Introduced Pujiang County, Sichuan Province kind early than 2000 Planting, Pujiang County in 2003 achieves does not knows that fire individual plant bears fruit more than 5 kilograms, up to 15 kilograms, Pujiang ginseng of coming for only 2003 See study personnel and just have more than 1000 person-times.Thereafter the county such as the Danleng, Jintang in Sichuan Province the most progressively introduces, inland river, another Sichuan, Chongqing, Yunnan, Jiangxi, Hunan, zhejiang and other places also has local plantation.Use after high-grafting technical graft, do not know fiery miscellaneous Citrus chachiensis Hort. in river western basin To show as fruit big, Mean Fruit Weight 200 grams, maximum more than 400 grams.Fruit obovate, has the short neck of projection more;Peel yellow orange Color, early October starts coloring, and early December colours completely;Peel yellowish orange, really face is slightly thick, and easy peeling, fruit juice pol is 13% Above, up to 17%, taste is the sweetest;Next year 2~March are ripe, and local flavor is fabulous.The fruit of ugly Citrus chachiensis Hort., is single flower the most entirely, but also has Total shape flower, the size of flower is shown in big with ponkan than clearly.Ugly Citrus chachiensis Hort. has pollen, but pollen amount is few, and majority is malformed flower, and seedless fruit rate is very Height, even if there being core, seed number is also few, and parthenocarpy is strong, single fruit weight 200-280g, is the big fruit shape in m andarin.Really shape Obovate or oblate spheroid, fruit shape index 1.00-2.00.
Retrieve the documents below plantation about ugly Citrus chachiensis Hort.:
Chinese patent, application number: 201410330685.6;Applicant: Jiangxi Wang Pin agricultural science and technology development corporation, Ltd.;Invention name Claim: do not know fiery miscellaneous Citrus chachiensis Hort. off-season cultivation method;Summary: the present invention relates to one and do not know fiery miscellaneous Citrus chachiensis Hort. off-season cultivation method, the party Method utilizes the characteristic not knowing the fiery indefinite flower of miscellaneous Citrus chachiensis Hort., by ploughing deeply the soil treatment such as applying organic manure, Cover with weed of improving the soil, divides and executes in season Using different classes of fertilizer, point season irrigates, and divides and season sprays foliage fertilizer, the operations such as point season prunes, fruit retention, it is achieved do not know fiery miscellaneous Citrus chachiensis Hort. one Set and three fruits of three bow structures within 1 year, can be opened, i.e. 3, winter of blooming in April ripe, bloom summer and autumn ripe in May next year, opens autumn Spend August next year ripe.And anti-season fruit single fruit is great, improves quality, more due to the particularity of anti-season, ripe forward market Almost without fresh citrus fruit on field, and then enhance competitiveness, obtain preferable economic benefit.
Ugly Citrus chachiensis Hort. possesses preferable economic benefit, and now in inland river, Sichuan, Chongqing, Yunnan, Jiangxi, Hunan, zhejiang and other places has Local plantation, the ugly Citrus chachiensis Hort. Seedling of anosis insect, by studying for many years, is the most successfully cultivated successfully, and is intended in Guangxi a large amount of by the present inventor Plantation.
Summary of the invention
It is an object of the invention to the implantation methods of a kind of ugly Citrus chachiensis Hort., utilize the cultivation of rootstock seedling and Shoot-tip Grafting technology successfully to train Bring out virus-free ugly Citrus chachiensis Hort. plant, and plant in Guangxi successfully, abundant local industrial crops and objective for local farmers creation Economic benefit.
The implantation methods of a kind of ugly Citrus chachiensis Hort., including the cultivation of nursery stock, selection of land, whole, definite value, rich water quality management, field management, disease The step of pest control;Wherein, the cultivation of nursery stock includes cultivation and two steps of Shoot-tip Grafting of rootstock seedling.
The cultivation of described rootstock seedling: selecting the Guangxi Citrus chachiensis Hort. 4-5 age of tree, robust growth, trunk are thick, without pest and disease damage The seed of Gan Shu, for reducing material contamination source, when selecting seed, should select within continuous more than three days, carry out after fair weather;With After tap water cleans up, first with the alcoholic solution sterilization 6-8min that mass concentration is 50%, it is the laundry of 10% by mass concentration Powder soaks 10 min, with aseptic water washing 3-5 time, and constantly vibrates, peels off interior episperm, be then seeded in ready substantially In culture medium MS, after 27 ± 0.5 DEG C of light culture 13-16 days, obtain etiolated seedling, after etiolated seedling is placed in next sky of nature light, As stock;
Described Shoot-tip Grafting: take the long stem apex to 1~2cm Sichuan Pujiang ugly Citrus chachiensis Hort. kind of new extraction, rinse well with tap water, Carrying out disinfection with the detergent that mass concentration is 5%, immersion limit, limit is stirred, aseptic water washing 3~5 times;With 0.5 mg/L NAA Pretreatment 5 min, then with 1.2mg/L6-BA min pretreatment 12 min;By pretreated stem apex distillation washing 2 times, transfer With the 9min that sterilizes in the mercuric chloride solution of 0.1% before connecing, immersion limit, limit is stirred, and uses aseptic water washing 3~5 times the most again;In nothing Under the conditions of bacterium, first cut part stem end and the root system of stock with the grafting knife through sterilization, stay stem length 2 cm, root length 3~4 Cm: at the mm of top 2~4, cut out a groove face on stock, as deep as center pillar, takes 0.14~0.18mm size, containing 2~3 The Shoot-tip Grafting of phyllopodium is on stock;The test tube Seedling grafted is put into off-the-shelf culture medium 1. in;Spike length waiting goes out Carry out except sprouting after 2~4 leaves, be transferred to culture medium and 2. continue to cultivate;Nursery stock to be grafted moves on to warmhouse booth when growing 5 blades After seedling exercising 2d, wash down the grains of sand with sterilized water during removal seedling, plant in non-toxic base;When nursery stock length to 12-15 cm, claim it For " cultivation Seedling ", " cultivation Seedling " is transplanted in orchard.
Described selection of land is: select fertile, and soil pH value is at 5.5-7.0;The physical features gradient is less than 25 degree, traffic, water source The good place of condition is as planting base;
Described is wholely: using powder ridge machine powder ridge wholely, tilth is 60-80cm;
Described field planting is: according to the density of spacing in the rows 2.5 × line-spacing 3m " cultivation Seedling " dig planting pit, cave each 80cm deep, wide, so Rear pressure fertilizer 50cm is deep, and backfill soil 40cm height is planted;By putting into planting pit central authorities after the root system pruning of nursery stock when planting, unfold Root system, righting, banket while the most upwards carry Seedling, tread, make root system touch with soil;Water foot and determine root water, do around seedling The tree dish of 1m, covers with bran shell, improves soil temperature, keeps loose aeration status;
Described rich water quality management is: purity nitrogen 100-400g is executed in the every strain every year of 1-3 treelet, also needs to apply phosphorus and potassium, nitrogen: phosphorus: potassium Part by weight be 1.0: 0.4-0.5: 1.0;
Tree emphasis of bearing fruit uses sufficient fertile and fruit-promoting fertilizer after adopting, and adopts rear fertilizer and accounts for the 30%~50% of annual dose, based on fertilizer, takes Join compound fertilizer, apply after one week in fruit picking;The weakest for tree vigo(u)r and fertile that use deficiency after fruit picking in the first half of the year, execute a small amount of Bud before fertile, fertile before bud coordinate with fertilizer and inorganic fertilizer, in terms of purity nitrogen, fertilizer is the 50%-70% of fertile total dose before accounting for bud, In inorganic fertilizer, the part by weight of nitrogen, phosphorus and potassium controls 1: 0.8: 0.8;Fruit-promoting fertilizer coordinates with fertilizer and inorganic fertilizer;
Described field management is: control the spring tip, summer shoot of erasing, and protects leaf flower and fruit protecting, flower and fruit thinning after steady fruit, strengthens tree vigo(u)r, Cultivate strong tree;Tanaka is beaten the grass by hay mover, and grass goes back to field and becomes again green fertilizer;Infrared green in fruit is overlapped by 12-1 month Double-ply bag survive the winter, when the 2-3 month to next year plucks, increase fruit sugariness;
The described prevention and control of plant diseases, pest control: use trade name " the green grain husk of Korea S SK " mineral oil 300-400 that SK company of Korea S produces Fruit tree is sprayed by times solution, is mainly used in preventing and treating red spider, makes fruit color more bright-coloured, and nontoxic non agricultural chemical residuum becomes Organic fruits.The network address of mineral oil is http://china.makepolo.com/product-detail/ 100242145640.html, effective ingredient is mineral oil, and active constituent content is 99%, micro-poison, and dosage form is cream.
Above-described through sterilization grafting knife refer to by grafting knife the Eusol that mass concentration is 10-20%, Mass concentration is the liquor natrii hypochloritis of 5.25% and mass concentration is 2% sodium hydroxide and mass concentration is the mixed of 2% formalin Close any one solution soaking 1-2min in solution.
1. above-described culture medium is MS+6-BA0.35mg/L+GA30.10mg/L+NAA0.05mg/L+2% sugar Honey, pH 5.8-6.0.
2. above-described culture medium is 1/4MS+ IBA0.5mg/L+ ABT 0.2mg/L+2% molasses, pH 5.8- 6.0。
Above-described fertilizer is made up of the raw material of following parts by weight: 70-80 weight portion sheep stool, 50-60 weight portion Pig manure, 15-20 weight portion humic acid, 5-10 weight portion chitosan, 40-50 weight portion Caulis Sacchari sinensis straw, 15-20 weight portion vegetation Ash, 25-30 weight portion tea bran and 1-2 weight portion actinomycetes live bacteria agent.
The preparation method of fertilizer is: by weight, by sheep stool, pig manure, humic acid, chitosan, Caulis Sacchari sinensis straw, vegetation Ash and tea bran mix homogeneously, obtain mixture, adds parts by weight actinomycetes live bacteria agent in the mixture, controls moisture at 35%- 50%, mix homogeneously, build heap, at normal temperatures fermentation 15-20 days.
Above-described adopt in rear fertilizer containing compound fertilizer's percentage by weight be 20-30%, wherein N >=8%, P2O5≥6%、K2O≥ 6%;Fertilizer percentage by weight >=50%;
Above-described fruit-promoting fertilizer is made up of the raw material of following part by weight: 55% fertilizer+16% potash fertilizer+12% phosphate fertilizer+12% nitrogen Fertile+1% calcareous fertilisers+1% fertiliser containing magnesium+1% boron fertilizer+1% zinc fertilizer+1% iron fertilizer.
Sheep stool is a kind of well fertilizer by fermentation, uses it to make fertilizer, can improve soil property, is to prevent soil floor Knot, economic worth is fine.
Pig manure quality is relatively thin, and containing more organic matter and npk nutrient, pig manure decomposes relatively slow, suitably makees base manure.
Humic acids agriculturally, is combined the humic acid fertilizer made (such as: neutralize with ammonia with elements such as nitrogen, phosphorus, potassium Humic acids can be made into ammonium humate fertilizer), there is fertilizer synergistic, improve soil, stimulate plant growth, improve agricultural product quality etc. Function;Nitro humic acid can be used as raising rice seedlings regulating acid agent;Humic acids magnesium, zinc humic acid, humic acid urea ferrum are supplementing respectively Good effect is had on soil magnesium deficiency, Semen Maydis zinc deficiency, Chlorosis in Fruit trees;Humic acids and the combined use of pesticides such as nitrofen, atrazine, permissible Improve drug effect, suppression residual hazard;Sodium humate is effective to treatment apple tree canker.
Chitosan is natural plant nutrient growth promoter--the raw material of foliage fertilizer, chitosan the foliage fertilizer compounded, Plant insecticide can be given, disease-resistant, play the effect of fertilizer, plant and animal residues and minor metallic element in soil can be decomposed again, from And it is converted into the nutrient of plant, strengthen via plant immunity, promote the health of plant;Containing abundant albumen in Crusta Penaeus seu Panulirus, Carapax Eriocheir sinensis Matter, trace element, after animal eats absorption, have good nutritive value.
Caulis Sacchari sinensis straw straw is one of important straw kind, containing nutrient necessary to crop have N, P, K, Ca, S etc..Under optimum conditions by the effect of soil microorganism, these elements are in mineralising returns soil, for Crop Utilize.
Tea bran, owing to the protein content of tea seed cake is higher, is a kind of efficient organic fertilizer, is widely used in crops and fruit tree In plantation, excellent.Tea bran mixes with other organic fertilizers and makees base manure, it is also possible to double control subterranean pest-insect such as prevent and treat little weevil, Holotrichia diomphalia Bates, Gryllotalpa etc..
In nursery stock transplanting, coordinate fertilizer to make base manure with plant ash, or mix the grass of 5%-20% when preparing Nutrition Soil Wood ash, not only can increase the available nutrient of base fertilizer and Nutrition Soil, promotes root growth, also alleviates the effect of pest and disease damage.
" the green grain husk of Korea S SK " is produced by SK company of Korea S, is a kind of control insect, efficient, the low toxicity mineral oil breast of evil demodicid mite Oil, is assessed as food stage agricultural spray oil by U.S. food management board.The network address of mineral oil is http: // China.makepolo.com/product-detail/100242145640.html, effective ingredient is mineral oil, effective ingredient Content is 99%, micro-poison, and dosage form is cream.
Green grain husk has all features of environmentally friendly pesticide: low toxicity, to person poultry safety;Little molecule may volatilize, permissible By microbial decomposition Cheng Shui and carbon dioxide, the most do not destroy ecological environment;Low to the lethality of natural enemy;Do not stimulate other The big generation of insect;Suffocating parasite killing with physics, be not never poison, therefore insect does not produce resistance to it;It is main for using green grain husk Pest integrative Therapeutic Method, it is possible to decrease the times for spraying of a year, reduces the destruction of environment and saves agricultural chemicals expense use;Improve, improve Fruit, quality of vegetable, improve fruit appearance, and non agricultural chemical residuum, is therefore highly suitable for " nuisanceless " vegetable, fruit base use Medicine.Additionally, green grain husk is in addition to being used alone pest control, it is also possible to as the spreader-sticker of insecticide, with chemistry, plant, micro-life Thing insecticide is used in mixed way, and improves these insecticides adhesion strength on plant and polypide, thus improves insecticidal effect.
The insecticidal mechanism of green grain husk: mineral oil can form oil film, sealed porosity on polypide, makes insect die of asphyxiation, or Entered pore by capillarity and kill off the insect pests.Green grain husk can change insect and find the ability of host.Herbivore insect mainly with On its foot, feeler, mouthpart and abdominal part, fine mechanorecepter differentiates host plant and decision is laid eggs and whether takes food.Green grain husk is formed Oil film, encloses these mechanorecepters, hinders the resolving ability of insect, thus reduces significantly and lay eggs and take food.Insect cannot Distinguish that it is if appropriate for taking food and laying eggs.Insect after contacting with the oil film on blade face, most before taking food and laying eggs from Open and post thing.
Test through Guangxi mandarin orange institute: green clever 200 times of liquid, 300 times of liquid, 400 times of liquid are used for preventing and treating citrus red mite.Medicine Rear 1d, 3d, 21d and 40d drug effect difference is the most notable.If green grain husk all has extremely strong lethal effect to one-tenth, children demodicid mite, drug effect is rapid, After medicine, 1d preventive effect is up to 98.2%~99.6%, and the phase that effectively controls, up to more than 40d, is that currently preventing and treating citrus red mite ideal is produced Product.
Compared with prior art, the positive effect of the present invention is:
1, the present invention provides the implantation methods of a kind of ugly Citrus chachiensis Hort., the cultivation of rootstock seedling and shoot-tip grafting plantlet to select different kinds;Anvil Wood Seedling selects by the Guangxi Citrus chachiensis Hort. 4-5 age of tree, and robust growth, trunk be thick, without the seed of Gan Shu of pest and disease damage, strictly controls Breeding condition, is cultivated into Resistant rootstock Seedling, shoot-tip grafting plantlet Sichuan Pujiang ugly Citrus chachiensis Hort. kind, and is utilized Shoot-tip Grafting skill Art, detoxic seedling of cultivating fine seed strains, it is often more important that breeding detoxic seedling is planted experimentally successfully in Guangxi, fertilising is adopted as inorganic fertilizer and fertilizer Mixing, the prevention and control of plant diseases, pest control uses " the green grain husk of Korea S SK " mineral oil, the ugly Citrus chachiensis Hort. Mean Fruit Weight of production more than 200 grams, maximum single fruit Weigh more than 350 grams, fruit juice pol more than 14%, up to 18%, meat soft and succulency, cyst wall is the most thin and flexible, and mouthfeel is crisp sweet, Quality is excellent, and consumer is profuse in praise.And the cultivation of detoxic seedling successfully decreases the generation of ugly Citrus chachiensis Hort. disease, improve yield and product Matter, promotes the Sustainable Healthy Development of local ugly Citrus chachiensis Hort. plant husbandry, creates objective economic benefit for local farmers.
2, the implantation methods of the present invention, in rich water quality management step, tree emphasis of bearing fruit uses sufficient fertile and fruit-promoting fertilizer after adopting, because tree Body recovers tree vigo(u)r because " puerperal " is badly in need of, and pumping summer shoot and bud differentiation need again to consume substantial amounts of nutrient substance, adopt so to use sufficient Rear fertilizer;And in fruit-promoting fertilizer, use sufficient azophoska the most in time, be especially focused on using sufficient potash fertilizer, promote Fruit, more than supplement calcium Trace element in magnesium boron zinc etc., promotees the strong fruit of the swollen fruit of Gen Baoye, replenishes the calcium, promote root system development, and fruit is solid, and prevention sun fruit carries The high hardness of fruit, reduces later stage dehiscent fruit;Magnesium supplement, promotes that blade is emerald green, reduces the yellow base of a fruit shedding that yellow leaf causes;Mend boron zinc, promote Bud differentiation in the coming year, reduces biennial bearing.Due in the methods of the invention, heavy dressing fertilizer, increase ugly Citrus chachiensis Hort. sugariness, make sugar content Up to 18%.
3, can be propagated by grafting knife, so in the present invention, by grafting knife first due to ugly Citrus chachiensis Hort. exocortis viroid Through sterilization, go the most again to cut budling.
4, the present invention uses " the green grain husk of Korea S SK " mineral oil to carry out the prevention and control of plant diseases, pest control, mainly by green grain husk mineral oil Oil film, sealed porosity can be formed on polypide, make insect die of asphyxiation, or be entered pore by capillarity and kill off the insect pests, Control insect, if through test green grain husk to becoming, children demodicid mite all has extremely strong lethal effect, drug effect is rapid, after medicine 1d preventive effect up to 98.2%~99.6%, the phase that effectively controls, up to more than 40d, is the ugly Citrus chachiensis Hort. red spider ideal product of current preventing and treating.
5, implantation methods of the present invention uses sheep stool, pig manure, humic acid, chitosan, Caulis Sacchari sinensis straw, plant ash, tea bran and puts The fertilizer that line bacterium live bacteria agent is made, can be effectively improved the soil property environment of Guangxi self-restraint Karst Mountains Desertification Area so that have in soil Machine matter content is greatly increased, moreover it is possible to preventing and treating subterranean pest-insect, activating soil, promotes root growth.Each material in fertilizer is all Possesses value all over the body, as sheep stool prevents the hardening soil;Pig manure quality is relatively thin, containing more organic matter and npk nutrient; Humic acids has fertilizer synergistic, improves soil, stimulates plant growth, improves the functions such as agricultural product quality;Chitosan can be planted Thing parasite killing, disease-resistant, play the effect of fertilizer, plant and animal residues and minor metallic element in soil can be decomposed again;Caulis Sacchari sinensis straw contains Nutrient necessary to crop is had to have N, P, K, Ca, S;Tea bran mixes with other organic fertilizers and makees base manure, it is also possible to double control ground Lower insect such as prevents and treats little weevil, Holotrichia diomphalia Bates, Gryllotalpa etc.;Coordinate fertilizer to make base manure with plant ash, not only can increase base fertilizer and nutrition The available nutrient of soil, promotes root growth, also alleviates the effect of pest and disease damage.
Detailed description of the invention
Embodiment 1
The implantation methods of a kind of ugly Citrus chachiensis Hort., including the cultivation of nursery stock, selection of land, whole, definite value, rich water quality management, field management, pest and disease damage The step of preventing and treating;Wherein, the cultivation of nursery stock includes cultivation and two steps of Shoot-tip Grafting of rootstock seedling.
The cultivation of described rootstock seedling: selecting the Guangxi Citrus chachiensis Hort. 4-5 age of tree, robust growth, trunk are thick, without pest and disease damage The seed of Gan Shu, for reducing material contamination source, when selecting seed, should select within continuous more than three days, carry out after fair weather;With After tap water cleans up, first with the alcoholic solution sterilization 6-8min that mass concentration is 50%, it is the laundry of 10% by mass concentration Powder soaks 10 min, with aseptic water washing 3-5 time, and constantly vibrates, peels off interior episperm, be then seeded in ready substantially In culture medium MS, after 27 ± 0.5 DEG C of light culture 13-16 days, obtain etiolated seedling, after etiolated seedling is placed in next sky of nature light, As stock;
Described Shoot-tip Grafting: take the long stem apex to 1~2cm Sichuan Pujiang ugly Citrus chachiensis Hort. kind of new extraction, rinse well with tap water, Carrying out disinfection with the detergent that mass concentration is 5%, immersion limit, limit is stirred, aseptic water washing 3~5 times;With 0.5 mg/L NAA Pretreatment 5 min, then with 1.2mg/L6-BA min pretreatment 12 min;By pretreated stem apex distillation washing 2 times, transfer With the 9min that sterilizes in the mercuric chloride solution of 0.1% before connecing, immersion limit, limit is stirred, and uses aseptic water washing 3~5 times the most again;In nothing Under the conditions of bacterium, first cut part stem end and the root system of stock with the grafting knife through sterilization, stay stem length 2 cm, root length 3~4 Cm: at the mm of top 2~4, cut out a groove face on stock, as deep as center pillar, takes 0.14~0.18mm size, containing 2~3 The Shoot-tip Grafting of phyllopodium is on stock;The test tube Seedling grafted is put into off-the-shelf culture medium 1. in;Spike length waiting goes out Carry out except sprouting after 2~4 leaves, be transferred to culture medium and 2. continue to cultivate;Nursery stock to be grafted moves on to warmhouse booth when growing 5 blades After seedling exercising 2d, wash down the grains of sand with sterilized water during removal seedling, plant in non-toxic base;When nursery stock length to 12-15 cm, claim it For " cultivation Seedling ", " cultivation Seedling " is transplanted in orchard.
Described selection of land is: select fertile, and soil pH value is at 5.5-7.0;The physical features gradient is less than 25 degree, traffic, water source The good place of condition is as planting base;
Described is wholely: using powder ridge machine powder ridge wholely, tilth is 60-80cm;
Described field planting is: according to the density of spacing in the rows 2.5 × line-spacing 3m " cultivation Seedling " dig planting pit, cave each 80cm deep, wide, so Rear pressure fertilizer 50cm is deep, and backfill soil 40cm height is planted;By putting into planting pit central authorities after the root system pruning of nursery stock when planting, unfold Root system, righting, banket while the most upwards carry Seedling, tread, make root system touch with soil;Water foot and determine root water, do around seedling The tree dish of 1m, covers with bran shell, improves soil temperature, keeps loose aeration status;
Described rich water quality management is: purity nitrogen 100g is executed in the every strain every year of 1-3 treelet, also needs to apply phosphorus and potassium, nitrogen: phosphorus: the weight of potassium Amount ratio is 1.0: 0.4-0.5: 1.0;
Tree emphasis of bearing fruit uses sufficient fertile and fruit-promoting fertilizer after adopting, and adopts rear fertilizer and accounts for the 30%~50% of annual dose, based on fertilizer, takes Join compound fertilizer, apply after one week in fruit picking;The weakest for tree vigo(u)r and fertile that use deficiency after fruit picking in the first half of the year, execute a small amount of Bud before fertile, fertile before bud coordinate with fertilizer and inorganic fertilizer, in terms of purity nitrogen, fertilizer is the 50%-70% of fertile total dose before accounting for bud, In inorganic fertilizer, the part by weight of nitrogen, phosphorus and potassium controls 1: 0.8: 0.8;Fruit-promoting fertilizer coordinates with fertilizer and inorganic fertilizer;
Described field management is: control the spring tip, summer shoot of erasing, and protects leaf flower and fruit protecting, flower and fruit thinning after steady fruit, strengthens tree vigo(u)r, Cultivate strong tree;Tanaka is beaten the grass by hay mover, and grass goes back to field and becomes again green fertilizer;Infrared green in fruit is overlapped by 12-1 month Double-ply bag survive the winter, when the 2-3 month to next year plucks, increase fruit sugariness;
The described prevention and control of plant diseases, pest control: use trade name " the green grain husk of Korea S SK " mineral oil 300-400 that SK company of Korea S produces Fruit tree is sprayed by times solution, is mainly used in preventing and treating red spider, makes fruit color more bright-coloured, and nontoxic non agricultural chemical residuum becomes Organic fruits.
The above-described grafting knife through sterilization refers to grafting knife in mass concentration be the Eusol of 10-20% Soak 1-2min.
1. above-described culture medium is MS+6-BA0.35mg/L+GA30.10mg/L+NAA0.05mg/L+2% sugar Honey, pH 5.8-6.0.
2. above-described culture medium is 1/4MS+ IBA0.5mg/L+ ABT 0.2mg/L+2% molasses, pH 5.8- 6.0。
Above-described fertilizer is made up of the raw material of following parts by weight: 70 weight portion sheep stool, 50 weight portion pig manures, 15 Weight portion humic acid, 5 weight portion chitosans, 40 weight portion Caulis Sacchari sinensis straws, 15 weight portion plant ash, 25 weight portion tea bran and 1 weights Amount part actinomycetes live bacteria agent.
The preparation method of fertilizer is: by weight, by sheep stool, pig manure, humic acid, chitosan, Caulis Sacchari sinensis straw, vegetation Ash and tea bran mix homogeneously, obtain mixture, adds parts by weight actinomycetes live bacteria agent in the mixture, controls moisture at 35%- 50%, mix homogeneously, build heap, at normal temperatures fermentation 15-20 days.
Above-described adopt in rear fertilizer containing compound fertilizer's percentage by weight be 20-30%, wherein N >=8%, P2O5≥6%、K2O≥ 6%;Fertilizer percentage by weight >=50%;
Above-described fruit-promoting fertilizer is made up of the raw material of following part by weight: 55% fertilizer+16% potash fertilizer+12% phosphate fertilizer+12% nitrogen Fertile+1% calcareous fertilisers+1% fertiliser containing magnesium+1% boron fertilizer+1% zinc fertilizer+1% iron fertilizer.
Embodiment 2
The implantation methods of a kind of ugly Citrus chachiensis Hort., including the cultivation of nursery stock, selection of land, whole, definite value, rich water quality management, field management, pest and disease damage The step of preventing and treating;Wherein, the cultivation of nursery stock includes cultivation and two steps of Shoot-tip Grafting of rootstock seedling.
The cultivation of described rootstock seedling: selecting the Guangxi Citrus chachiensis Hort. 4-5 age of tree, robust growth, trunk are thick, without pest and disease damage The seed of Gan Shu, for reducing material contamination source, when selecting seed, should select within continuous more than three days, carry out after fair weather;With After tap water cleans up, first with the alcoholic solution sterilization 6-8min that mass concentration is 50%, it is the laundry of 10% by mass concentration Powder soaks 10 min, with aseptic water washing 3-5 time, and constantly vibrates, peels off interior episperm, be then seeded in ready substantially In culture medium MS, after 27 ± 0.5 DEG C of light culture 13-16 days, obtain etiolated seedling, after etiolated seedling is placed in next sky of nature light, As stock;
Described Shoot-tip Grafting: take the long stem apex to 1~2cm Sichuan Pujiang ugly Citrus chachiensis Hort. kind of new extraction, rinse well with tap water, Carrying out disinfection with the detergent that mass concentration is 5%, immersion limit, limit is stirred, aseptic water washing 3~5 times;With 0.5 mg/L NAA Pretreatment 5 min, then with 1.2mg/L6-BA min pretreatment 12 min;By pretreated stem apex distillation washing 2 times, transfer With the 9min that sterilizes in the mercuric chloride solution of 0.1% before connecing, immersion limit, limit is stirred, and uses aseptic water washing 3~5 times the most again;In nothing Under the conditions of bacterium, first cut part stem end and the root system of stock with the grafting knife through sterilization, stay stem length 2 cm, root length 3~4 Cm: at the mm of top 2~4, cut out a groove face on stock, as deep as center pillar, takes 0.14~0.18mm size, containing 2~3 The Shoot-tip Grafting of phyllopodium is on stock;The test tube Seedling grafted is put into off-the-shelf culture medium 1. in;Spike length waiting goes out Carry out except sprouting after 2~4 leaves, be transferred to culture medium and 2. continue to cultivate;Nursery stock to be grafted moves on to warmhouse booth when growing 5 blades After seedling exercising 2d, wash down the grains of sand with sterilized water during removal seedling, plant in non-toxic base;When nursery stock length to 12-15 cm, claim it For " cultivation Seedling ", " cultivation Seedling " is transplanted in orchard.
Described selection of land is: select fertile, and soil pH value is at 5.5-7.0;The physical features gradient is less than 25 degree, traffic, water source The good place of condition is as planting base;
Described is wholely: using powder ridge machine powder ridge wholely, tilth is 60-80cm;
Described field planting is: according to the density of spacing in the rows 2.5 × line-spacing 3m " cultivation Seedling " dig planting pit, cave each 80cm deep, wide, so Rear pressure fertilizer 50cm is deep, and backfill soil 40cm height is planted;By putting into planting pit central authorities after the root system pruning of nursery stock when planting, unfold Root system, righting, banket while the most upwards carry Seedling, tread, make root system touch with soil;Water foot and determine root water, do around seedling The tree dish of 1m, covers with bran shell, improves soil temperature, keeps loose aeration status;
Described rich water quality management is: purity nitrogen 100-400g is executed in the every strain every year of 1-3 treelet, also needs to apply phosphorus and potassium, nitrogen: phosphorus: potassium Part by weight be 1.0: 0.4-0.5: 1.0;
Tree emphasis of bearing fruit uses sufficient fertile and fruit-promoting fertilizer after adopting, and adopts rear fertilizer and accounts for the 30%~50% of annual dose, based on fertilizer, takes Join compound fertilizer, apply after one week in fruit picking;The weakest for tree vigo(u)r and fertile that use deficiency after fruit picking in the first half of the year, execute a small amount of Bud before fertile, fertile before bud coordinate with fertilizer and inorganic fertilizer, in terms of purity nitrogen, fertilizer is the 50%-70% of fertile total dose before accounting for bud, In inorganic fertilizer, the part by weight of nitrogen, phosphorus and potassium controls 1: 0.8: 0.8;Fruit-promoting fertilizer coordinates with fertilizer and inorganic fertilizer;
Described field management is: control the spring tip, summer shoot of erasing, and protects leaf flower and fruit protecting, flower and fruit thinning after steady fruit, strengthens tree vigo(u)r, Cultivate strong tree;Tanaka is beaten the grass by hay mover, and grass goes back to field and becomes again green fertilizer;Infrared green in fruit is overlapped by 12-1 month Double-ply bag survive the winter, when the 2-3 month to next year plucks, increase fruit sugariness;
The described prevention and control of plant diseases, pest control: use trade name " the green grain husk of Korea S SK " mineral oil 300-400 that SK company of Korea S produces Fruit tree is sprayed by times solution, is mainly used in preventing and treating red spider, makes fruit color more bright-coloured, and nontoxic non agricultural chemical residuum becomes Organic fruits.
The above-described grafting knife through sterilization refers to grafting knife in mass concentration be the liquor natrii hypochloritis of 5.25% Soak 1-2min.
1. above-described culture medium is MS+6-BA0.35mg/L+GA30.10mg/L+NAA0.05mg/L+2% sugar Honey, pH 5.8-6.0.
2. above-described culture medium is 1/4MS+ IBA0.5mg/L+ ABT 0.2mg/L+2% molasses, pH 5.8- 6.0。
Above-described fertilizer is made up of the raw material of following parts by weight: 75 weight portion sheep stool, 55 weight portion pig manures, 18 Weight portion humic acid, 8 weight portion chitosans, 45 weight portion Caulis Sacchari sinensis straws, 18 weight portion plant ash, 28 weight portion tea brans and 1.5 Weight portion actinomycetes live bacteria agent.
The preparation method of fertilizer is: by weight, by sheep stool, pig manure, humic acid, chitosan, Caulis Sacchari sinensis straw, vegetation Ash and tea bran mix homogeneously, obtain mixture, adds parts by weight actinomycetes live bacteria agent in the mixture, controls moisture at 35%- 50%, mix homogeneously, build heap, at normal temperatures fermentation 15-20 days.
Above-described adopt in rear fertilizer containing compound fertilizer's percentage by weight be 20-30%, wherein N >=8%, P2O5≥6%、K2O≥ 6%;Fertilizer percentage by weight >=50%;
Above-described fruit-promoting fertilizer is made up of the raw material of following part by weight: 55% fertilizer+16% potash fertilizer+12% phosphate fertilizer+12% nitrogen Fertile+1% calcareous fertilisers+1% fertiliser containing magnesium+1% boron fertilizer+1% zinc fertilizer+1% iron fertilizer.
Embodiment 3
The implantation methods of a kind of ugly Citrus chachiensis Hort., including the cultivation of nursery stock, selection of land, whole, definite value, rich water quality management, field management, pest and disease damage The step of preventing and treating;Wherein, the cultivation of nursery stock includes cultivation and two steps of Shoot-tip Grafting of rootstock seedling.
The cultivation of described rootstock seedling: selecting the Guangxi Citrus chachiensis Hort. 4-5 age of tree, robust growth, trunk are thick, without pest and disease damage The seed of Gan Shu, for reducing material contamination source, when selecting seed, should select within continuous more than three days, carry out after fair weather;With After tap water cleans up, first with the alcoholic solution sterilization 6-8min that mass concentration is 50%, it is the laundry of 10% by mass concentration Powder soaks 10 min, with aseptic water washing 3-5 time, and constantly vibrates, peels off interior episperm, be then seeded in ready substantially In culture medium MS, after 27 ± 0.5 DEG C of light culture 13-16 days, obtain etiolated seedling, after etiolated seedling is placed in next sky of nature light, As stock;
Described Shoot-tip Grafting: take the long stem apex to 1~2cm Sichuan Pujiang ugly Citrus chachiensis Hort. kind of new extraction, rinse well with tap water, Carrying out disinfection with the detergent that mass concentration is 5%, immersion limit, limit is stirred, aseptic water washing 3~5 times;With 0.5 mg/L NAA Pretreatment 5 min, then with 1.2mg/L6-BA min pretreatment 12 min;By pretreated stem apex distillation washing 2 times, transfer With the 9min that sterilizes in the mercuric chloride solution of 0.1% before connecing, immersion limit, limit is stirred, and uses aseptic water washing 3~5 times the most again;In nothing Under the conditions of bacterium, first cut part stem end and the root system of stock with the grafting knife through sterilization, stay stem length 2 cm, root length 3~4 Cm: at the mm of top 2~4, cut out a groove face on stock, as deep as center pillar, takes 0.14~0.18mm size, containing 2~3 The Shoot-tip Grafting of phyllopodium is on stock;The test tube Seedling grafted is put into off-the-shelf culture medium 1. in;Spike length waiting goes out Carry out except sprouting after 2~4 leaves, be transferred to culture medium and 2. continue to cultivate;Nursery stock to be grafted moves on to warmhouse booth when growing 5 blades After seedling exercising 2d, wash down the grains of sand with sterilized water during removal seedling, plant in non-toxic base;When nursery stock length to 12-15 cm, claim it For " cultivation Seedling ", " cultivation Seedling " is transplanted in orchard.
Described selection of land is: select fertile, and soil pH value is at 5.5-7.0;The physical features gradient is less than 25 degree, traffic, water source The good place of condition is as planting base;
Described is wholely: using powder ridge machine powder ridge wholely, tilth is 60-80cm;
Described field planting is: according to the density of spacing in the rows 2.5 × line-spacing 3m " cultivation Seedling " dig planting pit, cave each 80cm deep, wide, so Rear pressure fertilizer 50cm is deep, and backfill soil 40cm height is planted;By putting into planting pit central authorities after the root system pruning of nursery stock when planting, unfold Root system, righting, banket while the most upwards carry Seedling, tread, make root system touch with soil;Water foot and determine root water, do around seedling The tree dish of 1m, covers with bran shell, improves soil temperature, keeps loose aeration status;
Described rich water quality management is: purity nitrogen 100-400g is executed in the every strain every year of 1-3 treelet, also needs to apply phosphorus and potassium, nitrogen: phosphorus: potassium Part by weight be 1.0: 0.4-0.5: 1.0;
Tree emphasis of bearing fruit uses sufficient fertile and fruit-promoting fertilizer after adopting, and adopts rear fertilizer and accounts for the 30%~50% of annual dose, based on fertilizer, takes Join compound fertilizer, apply after one week in fruit picking;The weakest for tree vigo(u)r and fertile that use deficiency after fruit picking in the first half of the year, execute a small amount of Bud before fertile, fertile before bud coordinate with fertilizer and inorganic fertilizer, in terms of purity nitrogen, fertilizer is the 50%-70% of fertile total dose before accounting for bud, In inorganic fertilizer, the part by weight of nitrogen, phosphorus and potassium controls 1: 0.8: 0.8;Fruit-promoting fertilizer coordinates with fertilizer and inorganic fertilizer;
Described field management is: control the spring tip, summer shoot of erasing, and protects leaf flower and fruit protecting, flower and fruit thinning after steady fruit, strengthens tree vigo(u)r, Cultivate strong tree;Tanaka is beaten the grass by hay mover, and grass goes back to field and becomes again green fertilizer;Infrared green in fruit is overlapped by 12-1 month Double-ply bag survive the winter, when the 2-3 month to next year plucks, increase fruit sugariness;
The described prevention and control of plant diseases, pest control: use trade name " the green grain husk of Korea S SK " mineral oil 300-400 that SK company of Korea S produces Fruit tree is sprayed by times solution, is mainly used in preventing and treating red spider, makes fruit color more bright-coloured, and nontoxic non agricultural chemical residuum becomes Organic fruits.
The above-described grafting knife through sterilization refers to grafting knife in mass concentration be that 2% sodium hydroxide is dense with quality Degree is any one solution soaking 1-2min in the mixed solution of 2% formalin.
1. above-described culture medium is MS+6-BA0.35mg/L+GA30.10mg/L+NAA0.05mg/L+2% sugar Honey, pH 5.8-6.0.
2. above-described culture medium is 1/4MS+ IBA0.5mg/L+ ABT 0.2mg/L+2% molasses, pH 5.8- 6.0。
Above-described fertilizer is made up of the raw material of following parts by weight: 80 weight portion sheep stool, 60 weight portion pig manures, 20 Weight portion humic acid, 10 weight portion chitosans, 50 weight portion Caulis Sacchari sinensis straws, 20 weight portion plant ash, 30 weight portion tea bran and 2 weights Amount part actinomycetes live bacteria agent.
The preparation method of fertilizer is: by weight, by sheep stool, pig manure, humic acid, chitosan, Caulis Sacchari sinensis straw, vegetation Ash and tea bran mix homogeneously, obtain mixture, adds parts by weight actinomycetes live bacteria agent in the mixture, controls moisture at 35%- 50%, mix homogeneously, build heap, at normal temperatures fermentation 15-20 days.
Above-described adopt in rear fertilizer containing compound fertilizer's percentage by weight be 20-30%, wherein N >=8%, P2O5≥6%、K2O≥ 6%;Fertilizer percentage by weight >=50%.
Above-described fruit-promoting fertilizer is made up of the raw material of following part by weight: 55% fertilizer+16% potash fertilizer+12% phosphate fertilizer+ 12% nitrogenous fertilizer+1% calcareous fertilisers+1% fertiliser containing magnesium+1% boron fertilizer+1% zinc fertilizer+1% iron fertilizer.
By said method in the advantage that ugly Citrus chachiensis Hort. has quality better, yield is high of Guangxi mountain planting, per mu yield in the 3rd year More than 2000kg, the 5th year more than per mu yield 2200kg, the 6th year more than per mu yield 2600kg, the 7th year more than per mu yield 2900kg, give Guangxi peasant household creates good harvest.

Claims (7)

1. an implantation methods for ugly Citrus chachiensis Hort., including the cultivation of nursery stock, selection of land, whole, definite value, rich water quality management, field management, disease pest The step of evil preventing and treating;It is characterized in that: the cultivation of nursery stock includes cultivation and two steps of Shoot-tip Grafting of rootstock seedling;
The cultivation of described rootstock seedling is: selecting the Guangxi Citrus chachiensis Hort. 4-5 age of tree, robust growth, trunk are thick, without pest and disease damage The seed of Gan Shu, for reducing material contamination source, when selecting seed, should select within continuous more than three days, carry out after fair weather;With certainly After water cleans up, first with the alcoholic solution sterilization 6-8min that mass concentration is 50%, it is the detergent of 10% by mass concentration Soak 10 min, with aseptic water washing 3-5 time, and constantly vibrate, peel off interior episperm, be then seeded in ready basic training Support in base MS, after 27 ± 0.5 DEG C of light culture 13-16 days, obtain etiolated seedling, after etiolated seedling is placed in next sky of nature light, use Make stock;
Described Shoot-tip Grafting is: takes the long stem apex to 1~2cm Sichuan Pujiang ugly Citrus chachiensis Hort. kind of new extraction, rinses with tap water dry Only, carrying out disinfection with the detergent that mass concentration is 5%, immersion limit, limit is stirred, aseptic water washing 3~5 times;Use 0.5 mg/L NAA pretreatment 5 min, then with 1.2mg/L6-BA min pretreatment 12 min;By pretreated stem apex distillation washing 2 times, With the 9min that sterilizes in the mercuric chloride solution of 0.1% before grafting, immersion limit, limit is stirred, the most again with aseptic water washing 3~5 times;? Under aseptic condition, first cut part stem end and the root system of stock with the grafting knife through sterilization, stay stem length 2 cm, root length 3~4 Cm: at the mm of top 2~4, cut out a groove face on stock, as deep as center pillar, takes 0.14~0.18mm size, containing 2~3 The Shoot-tip Grafting of phyllopodium is on stock;The test tube Seedling grafted is put into off-the-shelf culture medium 1. in;Spike length waiting goes out Carry out except sprouting after 2~4 leaves, be transferred to culture medium and 2. continue to cultivate;Nursery stock to be grafted moves on to warmhouse booth when growing 5 blades After seedling exercising 2d, wash down the grains of sand with sterilized water during removal seedling, plant in non-toxic base;When nursery stock length to 12-15 cm, claim it For " cultivation Seedling ".
The implantation methods of ugly Citrus chachiensis Hort. the most according to claim 1, it is characterised in that:
Described selection of land is: select fertile, and soil pH value is at 5.5-7.0;The physical features gradient is less than 25 degree, traffic, water condition Good place is as planting base;
Described is wholely: using powder ridge machine powder ridge wholely, tilth is 60-80cm;
Described field planting is: according to the density of spacing in the rows 2.5 × line-spacing 3m, above-mentioned " cultivation Seedling " is dug planting pit, and cave is deep, wide each 80cm, then pressure fertilizer 50cm is deep, and backfill soil 40cm height is planted;Put into after the root system pruning of nursery stock in planting pit when planting Centre, unfolds root system, righting, bankets while the most upwards carry Seedling, tread, and makes root system touch with soil;Water foot and determine root water, seedling Around make the tree dish of 1m, cover with bran shell;
Described rich water quality management is: purity nitrogen 100-400g is executed in the every strain every year of 1-3 treelet, also needs to apply phosphorus and potassium, nitrogen: phosphorus: potassium Part by weight be 1.0: 0.4-0.5: 1.0;
Tree emphasis of bearing fruit uses sufficient fertile and fruit-promoting fertilizer after adopting, and adopts rear fertilizer and accounts for the 30%~50% of annual dose, based on fertilizer, takes Join compound fertilizer, apply after one week in fruit picking;The weakest for tree vigo(u)r and fertile that use deficiency after fruit picking in the first half of the year, execute a small amount of Bud before fertile, fertile before bud coordinate with fertilizer and inorganic fertilizer, in terms of purity nitrogen, fertilizer is the 50%-70% of fertile total dose before accounting for bud, In inorganic fertilizer, the part by weight of nitrogen, phosphorus and potassium controls 1: 0.8: 0.8;Fruit-promoting fertilizer coordinates with fertilizer and inorganic fertilizer;
Described field management is: control the spring tip, summer shoot of erasing, and protects leaf flower and fruit protecting, flower and fruit thinning after steady fruit, strengthens tree vigo(u)r, Cultivate strong tree;Tanaka is beaten the grass by hay mover;In fruit is overlapped by 12-1 month, the double-ply bag of infrared green is survived the winter;
The described prevention and control of plant diseases, pest control: use trade name " the green grain husk of Korea S SK " mineral oil 300-400 that SK company of Korea S produces Fruit tree is sprayed by times solution, is mainly used in preventing and treating red spider.
The implantation methods of ugly Citrus chachiensis Hort. the most according to claim 1, it is characterised in that: the described grafting knife through sterilization refers to By grafting knife mass concentration be the Eusol of 10-20%, mass concentration be 5.25% liquor natrii hypochloritis and quality dense Any one solution soaking 1-2min in the mixed solution that degree is 2% sodium hydroxide and mass concentration is 2% formalin.
The implantation methods of ugly Citrus chachiensis Hort. the most according to claim 1, it is characterised in that: 1. described culture medium is MS+6- BA0.35mg/L+GA30.10mg/L+NAA0.05mg/L+2% molasses, pH 5.8-6.0;
2. described culture medium is 1/4MS+ IBA0.5mg/L+ ABT 0.2mg/L+2% molasses, pH 5.8-6.0.
The implantation methods of ugly Citrus chachiensis Hort. the most according to claim 2, it is characterised in that: described fertilizer is by following parts by weight Raw material composition: 70-80 weight portion sheep stool, 50-60 weight portion pig manure, 15-20 weight portion humic acid, 5-10 weight portion shell are poly- Sugar, 40-50 weight portion Caulis Sacchari sinensis straw, 15-20 weight portion plant ash, 25-30 weight portion tea bran and 1-2 weight portion actinomycetes viable bacteria Agent;
The preparation method of fertilizer is: by weight, by sheep stool, pig manure, humic acid, chitosan, Caulis Sacchari sinensis straw, plant ash and Tea bran mix homogeneously, obtains mixture, in the mixture add parts by weight actinomycetes live bacteria agent, control moisture at 35%-50%, Mix homogeneously, builds heap, at normal temperatures fermentation 15-20 days.
The implantation methods of ugly Citrus chachiensis Hort. the most according to claim 2, it is characterised in that: described adopts the weight Han compound fertilizer in rear fertilizer Percentage ratio is 20-30%, wherein N >=8%, P2O5≥6%、K2O≥6%;Fertilizer percentage by weight >=50%.
The implantation methods of ugly Citrus chachiensis Hort. the most according to claim 2, it is characterised in that: described fruit-promoting fertilizer is by following part by weight Raw material composition: 55% fertilizer+16% potash fertilizer+12% phosphate fertilizer+12% nitrogenous fertilizer+1% calcareous fertilisers+1% fertiliser containing magnesium+1% boron fertilizer+1% zinc fertilizer+1% ferrum Fertile.
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CN107750695A (en) * 2017-11-23 2018-03-06 荔浦县万家兴果蔬专业合作社 A kind of engrafting method of Ponkan
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陈世林等: "‘不知火’的主要特征及栽培技术要点", 《中国园艺文摘》 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106561374A (en) * 2016-10-09 2017-04-19 庞海宁 Cultivating method for selenium-enrichment granulated sugar orange
CN106613665A (en) * 2016-12-20 2017-05-10 广西金神农生态农业科技开发有限公司 Citrus planting method
CN107750695A (en) * 2017-11-23 2018-03-06 荔浦县万家兴果蔬专业合作社 A kind of engrafting method of Ponkan
CN108040788A (en) * 2017-12-26 2018-05-18 王静婵 A kind of implantation methods of beautiful millettia root
CN108314487A (en) * 2018-04-03 2018-07-24 广东丰康生物科技有限公司 A kind of preparation method of composite biological fertilizer for soil remediation
CN110999702A (en) * 2019-12-12 2020-04-14 江门绿壮生物科技有限公司 Ecological and friendly planting method of Xinhui oranges

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Application publication date: 20161012