CN103651361A - Plant wound protection liquid containing chitosan oligosaccharide - Google Patents

Plant wound protection liquid containing chitosan oligosaccharide Download PDF

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Publication number
CN103651361A
CN103651361A CN201410001007.5A CN201410001007A CN103651361A CN 103651361 A CN103651361 A CN 103651361A CN 201410001007 A CN201410001007 A CN 201410001007A CN 103651361 A CN103651361 A CN 103651361A
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China
Prior art keywords
liquor
protection
chitosan oligosaccharide
wound
trees
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CN201410001007.5A
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CN103651361B (en
Inventor
侯华民
张善学
陈坚
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Hainan Zhengye Biotechnology Co ltd
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Hainan Zhengye Zhongnong Hi Tech Co Ltd
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Priority claimed from CN201210058506.9A external-priority patent/CN102617224B/en
Publication of CN103651361A publication Critical patent/CN103651361A/en
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  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention relates to plant wound liquid containing chitosan oligosaccharide, which is characterized by comprising a plant growth regulator, wherein the regulator is one or more of naphthalene acetic acid and humic acid and accounts for 1 to 50 percent of the total weight of the liquid; and the chitosan oligosaccharide accounts for 0.0015 weight percent to 0.03 weight percent of the total weight of the liquid. The liquid can be prepared into any liquid preparation for spraying. One to five days before plants are trimmed, grafted or girdled, the liquid is used for spraying a wound. The liquid is used for apple trees, pear trees, peach trees, jujube trees, grape vines, mango trees, longan trees, lychee trees, mandarin trees, watermelons, gingko trees, lonicera japonica, jasmine, Chinese rose and the like.

Description

The plants wound protection liquor that contains chitosan oligosaccharide
The application is dividing an application of " a kind of plants wound protectant that contains chitosan oligosaccharide " application for a patent for invention, original application day: on March 7th, 2012, application number: 2012100585069.
Technical field
The invention belongs to plant protection field, particularly relate to a kind of plants wound protection liquor that contains chitosan oligosaccharide.
Background technology
For regulating plant structure, strengthen landscape effect, growth regulation and solid, adjusting nutrient and moisture running and distribution, improve ventilation and penetrating light etc., reach diseases prevention, increasing both production and income, need to fruit tree, flowers, view trees or vegetables be pruned, grafting or band girdle.Plant is being pruned, after the wound such as grafting or band girdle, plants wound position cannot form the environment isolated with the external world immediately, owing to may causing serious dehydration, and various plants pathogen may be invaded from wound, and cause that plant falls ill, wilt, cause tree vigo(u)r growth weak, even dead, to orchard worker, flower-grower or greening cause heavy economic losses, as apple tree is pruned the apple valsa canker causing, mango is pruned the mango gummosis causing, the Peach canker disease that peach pruning causes etc., so impelling plants wound protection is one of important content of the effective management of plant.Because chitosan oligosaccharide is a class plant immune class material, disease-resistant, the anti-low temperature of plant, volume increase, raising quality etc. are produced effect.At present, on market, also containing the plants wound of chitosan oligosaccharide, do not protect liquor.The present invention, in line with the thought of preventing and treating of putting prevention first, is intended to propose a kind of plants wound protection liquor that meets environmental requirement, is easy to reduce wound morbidity, promotion wound healing.
Summary of the invention
For solving, reduce wound morbidity, impel wound healing, the invention provides a kind of plants wound protection liquor that contains chitosan oligosaccharide.
Concrete, this law is bright provides a kind of plants wound that contains chitosan oligosaccharide to protect liquor, and the percentage by weight that chitosan oligosaccharide accounts for this protection liquor is 0.0015%-0.03%.Preferably, to account for the percentage by weight of this protection liquor be 0.005%-0.02% to chitosan oligosaccharide.
Especially concrete, the invention provides a kind of plants wound protection liquor that contains chitosan oligosaccharide, it is characterized in that the percentage by weight that chitosan oligosaccharide accounts for this protection liquor is 0.0015%-0.03%.Preferably, the percentage by weight that chitosan oligosaccharide accounts for this protection liquor is 0.005%-0.02%, also contains one or more in nutritional agents, plant growth regulator, bactericide.
In above-mentioned protection liquor, nutritional agents is preferably one or more in glycine, potassium dihydrogen phosphate, magnesium sulfate, boric acid, iron sulfate, calcium chloride, urea.
In above-mentioned protection liquor, conditioning agent is one or more in methyl α-naphthyl acetate, indolebutyric acid, humic acid.
In above-mentioned protection liquor; bactericide is chemical bactericide or botanical fungicide; chemical bactericide is one or more in Fluoxastrobin, thiophanate-methyl, tpn, copper rosinate, lime sulfur preferably, and botanical fungicide is one or more in the tissues such as Radix Isatidis, Chinese prickly ash, kuh-seng, China tree or extract preferably.
In above-mentioned protection liquor, preferred, 1-50%, the bactericide that 1-50%, the plant growth regulator that nutritional agents accounts for protection liquor gross weight accounts for protection liquor gross weight accounts for the 1-50% of protection liquor gross weight.
Above-mentioned protection liquor can also contain film forming agent, and preferred, binder for film formation is selected from one or more in polyvinylpyrrolidone, polyacrylamide, polypropylene, polyvinyl acetate.Preferably, film forming agent accounts for the 60-99% of protection liquor gross weight.
Above-mentioned protection liquor of the present invention can be made into any ointment preparation and liquid preparation, for spraying or smearing.Liquid preparation is preferably aqua, microemulsion, suspending agent or water dispersible granules, and ointment preparation is preferably paste.
In above-mentioned protection liquor, plant is the plant of any easy generation wound, preferably, is selected from apple tree, pear tree, peach, jujube tree, vine, mango, longan, litchi, mandarin tree, watermelon, ginkgo, honeysuckle, Jasmine or Chinese rose.Wherein said wound refers to the wound that plant is caused when pruning, grafting or band girdle.
The present invention also provides the purposes of above-mentioned protection for the protection of plants wound.
The present invention also provides the using method of above-mentioned plants wound protection liquor; it is characterized in that 1-5 days before vegetation pruning, grafting or band girdle; with described protection liquor, wound is sprayed, or after vegetation pruning, grafting or band girdle 1-5 days, on wound, smear above-mentioned protection liquor.Preferably, the described protection liquor of spraying for 2-3 days before vegetation pruning, grafting or band girdle, or within 1-2 days after vegetation pruning, band girdle or grafting, smear described protection liquor.
In the present invention, chitosan oligosaccharide is the mixture of the degree of polymerization that Glucosamine is formed by connecting by the Isosorbide-5-Nitrae-glycosidic bond high molecular polymer that is 2-30, and mean molecule quantity is 1000-3000 dalton.Chitosan oligosaccharide can be prepared by any means, such as following method: the mixture of the chitosan oligomer that the degree of polymerization that deacetylation prepares by methods such as mechanical degradation method, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation methods at more than 70% shitosan is 2-30.Or, by extract the mixture of the chitosan oligomer that the degree of polymerization obtain chitin and to prepare by methods such as mechanical degradation method, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation methods is 2-30 from fungal cell wall.Or, the mixture of the polymer that is 2-30 by the degree of polymerization that is polymerized by the chemosynthesis of Isosorbide-5-Nitrae-glycosidic bond by Glucosamine.Chitosan oligosaccharide is the mixture of the high molecular polymer of different polymer at present, that is to say in chitosan oligosaccharide to be not the oligosaccharides form of the single degree of polymerization, but the chitosan oligosaccharide monose of every kind of degree of polymerization all may exist.So no matter by which kind of method prepare; or the commercially available prod preparing by above-mentioned preparation method; as long as it is that Glucosamine passes through 1; the degree of polymerization that 4-glycosidic bond is formed by connecting is that 2-30, mean molecule quantity are that the mixture of the daltonian high molecular polymer of 1000-3000 is exactly chitosan oligosaccharide of the present invention; it can contain acetyl group; but the sugar unit of bonding acetyl group is no more than 40% amount, be preferably no more than 30% amount, be especially preferably no more than 20% amount.Commercially available chitosan oligosaccharide is all the chitosan oligosaccharide product that above-mentioned chitosan oligosaccharide term is set forth substantially at present.
Beneficial effect of the present invention:
The excellent effect of the plants wound protection liquor that the present invention has found to contain chitosan oligosaccharide, especially with the plant protection liquor that contains chitosan oligosaccharide after processing plant, plant morbidity is few, wound healing is fast, growing way is prosperous.Solved that crop is high at pruning, grafting or the band girdle incidence of disease, healing is slow, the problem a little less than tree vigo(u)r, improved ecology and the economic benefit of plant.
Embodiment
In order to understand the present invention, with embodiment, further illustrate the present invention below, but do not limit the present invention.
FORMULATION EXAMPLE 1:
Chitosan oligosaccharide 0.02g, binder for film formation polyacrylamide 400g, nutritional agents potassium dihydrogen phosphate 20g, conditioning agent indolebutyric acid 5g, fungicide chlorothalonil 100g, add water to 1000g, and stirring and evenly mixing can be smeared application.
FORMULATION EXAMPLE 2:
Chitosan oligosaccharide 0.03g, binder for film formation polyvinylpyrrolidone 500g, nutritional agents magnesium sulfate 50g, conditioning agent methyl α-naphthyl acetate 10g, bactericide thiophanate-methyl 150g, add water to 1000g, and stirring and evenly mixing can be smeared application.
FORMULATION EXAMPLE 3:
Chitosan oligosaccharide 0.05g, binder for film formation polyethylene 600g, nutritional agents iron sulfate 30g, conditioning agent humic acid 8g, bactericide thiophanate-methyl 80g, add water to 1000g, and stirring and evenly mixing can be smeared application.
FORMULATION EXAMPLE 4:
Chitosan oligosaccharide 0.03g, nutritional agents boric acid 30g, conditioning agent methyl α-naphthyl acetate 8g, bactericide Fluoxastrobin 80g, add water to 1000g, and stirring and evenly mixing gets final product spray applications.
FORMULATION EXAMPLE 5
Chitosan oligosaccharide 0.03g, bactericide Fluoxastrobin 80g, adds water to 1000g, and stirring and evenly mixing gets final product spray applications.
FORMULATION EXAMPLE 6
Chitosan oligosaccharide 0.05g, binder for film formation polyethylene 600g, nutritional agents iron sulfate 30g, adds water to 1000g, and stirring and evenly mixing can be smeared application.
FORMULATION EXAMPLE 7
Chitosan oligosaccharide 0.03g, conditioning agent methyl α-naphthyl acetate 10g, bactericide thiophanate methyl 150g, add water to 1000g, and stirring and evenly mixing can get final product spray applications.
FORMULATION EXAMPLE 8
Chitosan oligosaccharide 0.02g, nutritional agents potassium dihydrogen phosphate 20g, adds water to 1000g, and stirring and evenly mixing gets final product spray applications.
FORMULATION EXAMPLE 9
Chitosan oligosaccharide 0.05g, conditioning agent humic acid 8g, adds water to 1000g, and stirring and evenly mixing gets final product spray applications.
FORMULATION EXAMPLE 10
Chitosan oligosaccharide 0.08g, binder for film formation polyethylene 600g, adds water to 1000g, and stirring and evenly mixing can be smeared application.
Biological Examples 1:
Select apple tree, pear tree, peach, jujube tree, vine, mango, longan, litchi, mandarin tree, watermelon, ginkgo, honeysuckle, Jasmine, Chinese rose, same crop is divided into 2 groups, one group of processing of spraying, smears processing for one group.In the pruning processing of spraying in first 2 days, in pruning, within latter 2 days, smear processing, above processing is not all to be treated to contrast, the random listing mark wound of every processing number containing chitosan oligosaccharide.The different plant sprayings of " Invest, Then Investigate " in 30 days are processed wound morbidity number and smear and process wound healing number, and spraying result, in Table 1, is smeared result in Table 2.
As seen from Table 1; for different plants; melon and fruit, medicinal material or flowers; in wound spraying in first 2 days; after 30 days, the wound incidence of disease that contains chitosan oligosaccharide compositions-treated is all starkly lower than contrast, both lower than the wound incidence of disease of processing without chitosan oligosaccharide; visible, the control successful containing the Wound protection liquor of chitosan oligosaccharide to disease.As seen from Table 2; for the different plants for examination; within 2 days after wound, smear processing; after 30 days; the wound healing rate that contains chitosan oligosaccharide compositions-treated is all apparently higher than contrast; both higher than the wound healing rate of processing without chitosan oligosaccharide, visible, containing the Wound protection liquor of chitosan oligosaccharide, obviously promote the healing of wound.
Find in addition, no matter the compositions-treated that contains chitosan oligosaccharide, spray or smear processing, and plant growing way is vigorous.To sum up, the Wound protection liquor that contains chitosan oligosaccharide is sprayed and is smeared processing, can obviously reduce the morbidity of wound and promote wound healing.If agricultural, forestry pruning, grafting or band girdle during this chitosan oligosaccharide composition of extensive use, will bring obvious ecology and economic benefit.
The wound incidence that before the different vegetation prunings of table 1, spraying is processed
After the different vegetation prunings of table 2, smear the wound healing situation after Wound protection liquor

Claims (8)

1. the plants wound protection liquor that contains chitosan oligosaccharide; it is characterized in that protecting liquor to contain plant growth regulator; conditioning agent is one or more in methyl α-naphthyl acetate, humic acid, accounts for the 1-50% of protection liquor gross weight, and the percentage by weight that chitosan oligosaccharide accounts for protection liquor is 0.0015%-0.03%.
2. protection liquor according to claim 1, the percentage by weight that chitosan oligosaccharide accounts for this protection liquor is 0.005%-0.02%.
3. protection liquor according to claim 2, also contain nutritional agents, one or both in bactericide, wherein nutritional agents accounts for the 1-50% of protection liquor gross weight, bactericide accounts for the 1-50% of protection liquor gross weight, nutritional agents is glycine, potassium dihydrogen phosphate, magnesium sulfate, boric acid, iron sulfate, calcium chloride, one or more in urea, bactericide is chemical bactericide or botanical fungicide, chemical bactericide is selected from Fluoxastrobin, thiophanate methyl, tpn, copper rosinate, one or more in lime sulfur, botanical fungicide is selected from Radix Isatidis, Chinese prickly ash, kuh-seng, one or more in the tissues such as China tree or extract.
4. protection liquor according to claim 3, wherein protects liquor to can be made into any liquid preparation, for spraying.
5. protection liquor according to claim 4, wherein liquor is aqua, microemulsion, suspending agent or water dispersible granules.
6. the purposes for the protection of plants wound according to the protection liquor described in claim 1-5 any one.
7. the using method of protection liquor according to claim 6, is characterized in that 1-5 days before vegetation pruning, grafting or band girdle, with described protection liquor, wound is sprayed.
8. according to the using method of claim 7, the described protection liquor of spraying for 2-3 days before vegetation pruning, grafting or band girdle.
CN201410001007.5A 2012-03-07 2012-03-07 Plants wound protection liquor containing chitosan oligosaccharide Active CN103651361B (en)

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Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104211537A (en) * 2014-08-27 2014-12-17 武汉瑞泽园生物环保科技有限公司 Environment-friendly fertilizer for lotus and preparation method thereof
CN104230573A (en) * 2014-09-28 2014-12-24 淮南市业鹏苗木种植农民专业合作社 Nutrient solution for increasing grafting survival rate and preparation method thereof
CN104557189A (en) * 2014-09-12 2015-04-29 襄阳市风雅月季开发有限公司 Flower/tree grafting wound healing nutrient solution
CN104886157A (en) * 2015-05-08 2015-09-09 长阳勤劳农夫农产品有限公司 Grafting treatment agent and application thereof on grafting stock for solanaceae crops
CN105967890A (en) * 2016-06-02 2016-09-28 麻考 Plastic winding strip treating agent for grape grafting propagation
CN106467433A (en) * 2016-08-31 2017-03-01 蒋石林 Fruit tree diseases prevention promotees bud and keeps a full stand of seedings accelerator and its application
CN106576933A (en) * 2016-12-23 2017-04-26 庆阳敦博科技发展有限公司 Lichee top grafting method
CN106804605A (en) * 2015-12-02 2017-06-09 中国科学院大连化学物理研究所 A kind of wound healing agent for plants containing sodium alginate oligosaccharide and application thereof
CN107141058A (en) * 2017-04-27 2017-09-08 广西棕海园林工程有限公司 Plants wound renovation agent
CN107567929A (en) * 2017-09-30 2018-01-12 东兰县旺达板栗茶油种植专业合作社 A kind of tea oil tree implantation methods of high yield tea oil
CN107568225A (en) * 2017-09-30 2018-01-12 京博农化科技股份有限公司 One plant growth additive
CN107646576A (en) * 2017-10-09 2018-02-02 潍坊郭牌农业科技有限公司 A kind of Grafting watermelon method for culturing seedlings
CN108409442A (en) * 2018-05-10 2018-08-17 广德县东亭乡绘绿苗木种植家庭农场 A kind of Wound healing nutrient solution and preparation method thereof for grafting
CN109076870A (en) * 2018-11-07 2018-12-25 广西金花茶业有限公司 A kind of implantation methods of liana Jasmine
CN109197895A (en) * 2018-08-07 2019-01-15 广西田东县东辰农业开发有限公司 A kind of method of biocontrol of mango bleeding disease
CN109729861A (en) * 2019-03-25 2019-05-10 湖南省林大油茶有限公司 A kind of oil tea engrafting method
CN109743999A (en) * 2019-02-21 2019-05-14 安徽泓森高科林业股份有限公司 A kind of plant graft renovation agent
CN110149933A (en) * 2019-07-03 2019-08-23 潘文鋆 The grafting and breeding method of bergamot pear

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CN101161075A (en) * 2007-11-27 2008-04-16 李开森 Fruit tree wound healing protective agent
CN102187879A (en) * 2011-01-27 2011-09-21 刘福京 Method for preventing and treating fruit tree meloidogynosis by adopting carboxymethyl chitosan protective film

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
CN101161075A (en) * 2007-11-27 2008-04-16 李开森 Fruit tree wound healing protective agent
CN102187879A (en) * 2011-01-27 2011-09-21 刘福京 Method for preventing and treating fruit tree meloidogynosis by adopting carboxymethyl chitosan protective film

Cited By (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104211537B (en) * 2014-08-27 2016-06-08 武汉瑞泽园生物环保科技有限公司 Rhizoma Nelumbinis environment-friendly fertilizer and preparation method thereof
CN104211537A (en) * 2014-08-27 2014-12-17 武汉瑞泽园生物环保科技有限公司 Environment-friendly fertilizer for lotus and preparation method thereof
CN104557189A (en) * 2014-09-12 2015-04-29 襄阳市风雅月季开发有限公司 Flower/tree grafting wound healing nutrient solution
CN104230573A (en) * 2014-09-28 2014-12-24 淮南市业鹏苗木种植农民专业合作社 Nutrient solution for increasing grafting survival rate and preparation method thereof
CN104886157A (en) * 2015-05-08 2015-09-09 长阳勤劳农夫农产品有限公司 Grafting treatment agent and application thereof on grafting stock for solanaceae crops
CN104886157B (en) * 2015-05-08 2017-10-10 湖北勤劳农夫生态农业股份有限公司 A kind of grafting inorganic agent and the application on solanaceous crops graft stock
CN106804605A (en) * 2015-12-02 2017-06-09 中国科学院大连化学物理研究所 A kind of wound healing agent for plants containing sodium alginate oligosaccharide and application thereof
CN106804605B (en) * 2015-12-02 2019-10-25 中国科学院大连化学物理研究所 A kind of wound healing agent for plants and application thereof containing sodium alginate oligosaccharide
CN105967890A (en) * 2016-06-02 2016-09-28 麻考 Plastic winding strip treating agent for grape grafting propagation
CN106467433A (en) * 2016-08-31 2017-03-01 蒋石林 Fruit tree diseases prevention promotees bud and keeps a full stand of seedings accelerator and its application
CN106576933A (en) * 2016-12-23 2017-04-26 庆阳敦博科技发展有限公司 Lichee top grafting method
CN107141058A (en) * 2017-04-27 2017-09-08 广西棕海园林工程有限公司 Plants wound renovation agent
CN107567929A (en) * 2017-09-30 2018-01-12 东兰县旺达板栗茶油种植专业合作社 A kind of tea oil tree implantation methods of high yield tea oil
CN107568225A (en) * 2017-09-30 2018-01-12 京博农化科技股份有限公司 One plant growth additive
CN107646576A (en) * 2017-10-09 2018-02-02 潍坊郭牌农业科技有限公司 A kind of Grafting watermelon method for culturing seedlings
CN108409442A (en) * 2018-05-10 2018-08-17 广德县东亭乡绘绿苗木种植家庭农场 A kind of Wound healing nutrient solution and preparation method thereof for grafting
CN109197895A (en) * 2018-08-07 2019-01-15 广西田东县东辰农业开发有限公司 A kind of method of biocontrol of mango bleeding disease
CN109076870A (en) * 2018-11-07 2018-12-25 广西金花茶业有限公司 A kind of implantation methods of liana Jasmine
CN109076870B (en) * 2018-11-07 2020-11-20 广西金花茶业有限公司 Planting method of vine jasmine
CN109743999A (en) * 2019-02-21 2019-05-14 安徽泓森高科林业股份有限公司 A kind of plant graft renovation agent
CN109729861A (en) * 2019-03-25 2019-05-10 湖南省林大油茶有限公司 A kind of oil tea engrafting method
CN110149933A (en) * 2019-07-03 2019-08-23 潘文鋆 The grafting and breeding method of bergamot pear

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Address before: 571924 Fuchang village, Laocheng Development Zone, Chengmai County, Hainan Province

Patentee before: HAINAN ZHENGYE ZHONGNONG HIGH TECHNOLOGY Co.,Ltd.

CP03 Change of name, title or address