CN102613198B - Chitosan oligosaccharide agricultural composition for improving coloration of plants and application thereof and method by utilizing same to improve coloration of plants - Google Patents

Chitosan oligosaccharide agricultural composition for improving coloration of plants and application thereof and method by utilizing same to improve coloration of plants Download PDF

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CN102613198B
CN102613198B CN201210058562.2A CN201210058562A CN102613198B CN 102613198 B CN102613198 B CN 102613198B CN 201210058562 A CN201210058562 A CN 201210058562A CN 102613198 B CN102613198 B CN 102613198B
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chitosan oligosaccharide
plant
plants
coloration
improving
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CN102613198A (en
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侯华民
张善学
陈坚
陈泰龙
陈其确
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Hainan Zhengye Biotechnology Co ltd
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Hainan Zhengye Zhongnong Hi Tech Co Ltd
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Abstract

The invention relates to a chitosan oligosaccharide agricultural composition for improving coloration of plants and an application thereof and a method by utilizing the same to improve coloration of plants. The invention particularly provides a chitosan oligosaccharide agricultural composition for improving coloration of plants. The weight percentage of the chitosan oligosaccharide in the agricultural composition is 0.0015 to 0.015 percent and is preferentially 0.0025 percent to 0.01 percent. The chitosan oligosaccharide agricultural composition also comprises other ingredients such as one or multiple varieties of glycine, levulinic acid, glutamic acid, phenylalanine, tyrosine, acetylsalicylic acid, genistein, uniconazole, potassium sulphate, monobasic potassium phosphate, dipotassium phosphate, potassium phosphate, calcium chloride and boric acid. The plants include crops and flowers. An evaluation target of the plant coloration is a harvesting target or an ornamental target of the plant such as seeds, fruits, roots, stems, leaves and flowers.

Description

A kind of painted chitosan oligosaccharide agriculturally useful compositions of plant and uses thereof and method improved
Technical field
The invention belongs to agrochemical field, particularly relate to a kind of painted chitosan oligosaccharide agriculturally useful compositions of plant and uses thereof and method improved.
Background technology
Anthocyan is one of main pigment forming plant color, is plant secondary metabolism product, is that a class is extensively present in the water-soluble natural pigment in plant, and the multicoloured color major parts such as fruit, vegetables, flowers are relevant with it.Anthocyan forms more, and the color of plant is just more beautiful.In addition, anthocyan is the antidotal nutritious supplementary pharmaceutical of pure natural, is that mankind nowadays is found the most effective antioxidant, and in food industry at present, pigment used mostly is synthetic dyestuff, the long-term health of using meeting harm people, so natural colouring matter just more and more causes the concern of researcher.Increasing of Anthocyanin, quality of agricultural product improvement, sightseeing tour etc. are all had to effective facilitation, so gorgeous fruit, the vegetables and flowers of appearance luster is the main cause of the pleasing and persistent pursuit of consumer, also directly embodies its commodity performance always.
Improve at present the painted method of plant and mainly contain the plant illumination condition of improving, bagging, priming leaf, paving reflective membrane etc., but these methods or be subject to illumination effect, otherwise workload is large.In addition, spray methyl α-naphthyl acetate, ethrel, abscisic acid etc. and also can promote the painted of apple, but the arriving of crop respiratory climacteric cause reducing enduring store property because these plant growth substances can cause, shorten shelf life.The present invention is by the property research of chitosan oligosaccharide and composition thereof is found, chitosan oligosaccharide has special effect to the painted improvement of plant, and not only method is simple, and effect is remarkable.
Summary of the invention
For solving, improve that plant is paintedly limited to illumination condition, workload is large or the reason such as enduring store property is low, to the invention provides a kind ofly for improving painted agriculturally useful compositions of plant and uses thereof and method, in this agriculturally useful compositions, to comprise chitosan oligosaccharide.
Concrete, the invention provides a kind of for improving the painted chitosan oligosaccharide agriculturally useful compositions of plant, the percentage by weight that contains chitosan oligosaccharide in this agriculturally useful compositions is 0.0015%-0.015%, and preferred, the percentage by weight that chitosan oligosaccharide accounts for composition is 0.0025%-0.01%.
Concrete, the invention provides a kind of for improving the painted chitosan oligosaccharide agriculturally useful compositions of plant, the percentage by weight that contains chitosan oligosaccharide in this agriculturally useful compositions is 0.0015%-0.015%, preferably, the percentage by weight that chitosan oligosaccharide accounts for composition is 0.0025%-0.01%, also contain other compositions, other compositions are one or more in glycine, levulinic acid, glutamic acid, phenyl alanine, TYR, acetylsalicylic acid, Genistein, uniconazole P, potassium sulphate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, potassium phosphate, calcium chloride and boric acid.Preferably, other compositions are two or more in glycine, levulinic acid, glutamic acid, phenyl alanine, TYR, acetylsalicylic acid, Genistein, uniconazole P, potassium sulphate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, potassium phosphate, calcium chloride and boric acid.
Preferably, the gross weight of other compositions accounts for the 0.05-5% of composition total weight, preferred, accounts for 0.05-1%.
Above-mentioned this agriculturally useful compositions can be made any liquid preparation and any solid pharmaceutical preparation of agriculture use.Preferably, liquid preparation is aqua, microemulsion or suspending agent; Solid pharmaceutical preparation is preferably wetting powder or water dispersion granule.
The present invention also provides above-mentioned composition for improving the painted purposes of plant.
Especially concrete, the present invention also provides above-mentioned composition for improving the painted purposes of plant being caused by anthocyan.
The present invention also provides the above-mentioned agriculturally useful compositions of application for improving plant colorize method, it is characterized in that every 7-14 days, in seedling stage, the vegetative period of plant, bloom before and/or bloom after use this agriculturally useful compositions.Preferably every 10 days, then use 0-3 time, then the concentration of the concentration of using and front applied once is identical or different
In said method, this plant is any crop that need to improve plant outward appearance, comprises crop and flowers, preferably from plants such as apple, mango, grape, lichee, cherry, strawberry, red French beans, tomato, orchid, kaffir lilies.
In said method, the painted evaluation object of described plant is plant organ painted of any one-phase improve vegetation period, and these organs are seed, fruit, root, stem, leaf, flower.
In said method, the method for using refers to any effective common method when agricultural product are used, and includes but not limited to spray-on process, seed dressing method, soil treatment method etc.
In the present invention, chitosan oligosaccharide is the mixture of the degree of polymerization that Glucosamine is formed by connecting by the Isosorbide-5-Nitrae-glycosidic bond high molecular polymer that is 2-30, and mean molecule quantity is 1000-3000 dalton.Chitosan oligosaccharide can be prepared by any means, such as following method: the mixture of the chitosan oligomer that the degree of polymerization that deacetylation prepares by methods such as mechanical degradation method, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation methods at more than 70% shitosan is 2-30.Or, by extract the mixture of the chitosan oligomer that the degree of polymerization obtain chitin and to prepare by methods such as mechanical degradation method, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation methods is 2-30 from fungal cell wall.Or, the mixture of the polymer that is 2-30 by the degree of polymerization that is polymerized by the chemosynthesis of Isosorbide-5-Nitrae-glycosidic bond by Glucosamine.Chitosan oligosaccharide is the mixture of the high molecular polymer of different polymer at present, that is to say in chitosan oligosaccharide to be not the oligosaccharides form of the single degree of polymerization, but the chitosan oligosaccharide monose of every kind of degree of polymerization all may exist.So no matter by which kind of method prepare; or the commercially available prod preparing by above-mentioned preparation method; as long as it is that Glucosamine passes through 1; the degree of polymerization that 4-glycosidic bond is formed by connecting is that 2-30, mean molecule quantity are that the mixture of the daltonian high molecular polymer of 1000-3000 is exactly chitosan oligosaccharide of the present invention; it can contain acetyl group; but the sugar unit of bonding acetyl group is no more than 40% amount, be preferably no more than 30% amount, be especially preferably no more than 20% amount.Commercially available chitosan oligosaccharide is all the chitosan oligosaccharide product that above-mentioned chitosan oligosaccharide term is set forth substantially at present.
The painted variation that refers to that plant was occurred by object in color described in the present invention, relate to the reason that expires and change, the influence factor that has anthocyan, the influence that also has other compositions, the present invention finds by measuring the content of anthocyan the content that basic reason that chitosan oligosaccharide changes color has been to change the anthocyan in plant results object or required object.
Beneficial effect of the present invention:
The present invention has found for improving the painted agriculturally useful compositions that comprises chitosan oligosaccharide of plant, the chitosan oligosaccharide of particularly having found certain concentration is improving the painted special effect of plant, especially use the agriculturally useful compositions that comprises chitosan oligosaccharide after processing plant, not only improve coloring effect, commodity performance improves, and can not produce poisoning, non agricultural chemical residuum.The special contribution of the present invention is also to have found application chitosan oligosaccharide and painted good chance and the concentration of composition for improved plant by research, has greatly improved the commodity performance of plant.
Embodiment
In order to understand the present invention, with embodiment, further illustrate the present invention below, but do not limit the present invention.
Example of formulations 1
Get 0.05 gram of chitosan oligosaccharide, be diluted with water to 1000g, spray application.
Example of formulations 2
Get 0.15 gram of chitosan oligosaccharide, be diluted with water to 1000g, spray application.
Example of formulations 3
Get 0.09 gram of chitosan oligosaccharide, glycine 2g, boric acid 1g, is diluted with water to 1000g, spray application.
Example of formulations 4
Get 0.075 gram of chitosan oligosaccharide, levulinic acid 3g, TYR 0.5g, is diluted with water to 1000g, spray application.
Example of formulations 5
Get 0.075 gram of chitosan oligosaccharide, glutamic acid 1.5g, acetylsalicylic acid 1.5g, is diluted with water to 1000g, spray application.
Example of formulations 6
Get 0.095 gram of chitosan oligosaccharide, phenyl alanine 2g, Genistein 2.5g, is diluted with water to 1000g, spray application.
Example of formulations 7
Get 0.08 gram of chitosan oligosaccharide, potassium sulphate 0.5g, potassium dihydrogen phosphate 0.5g, calcium chloride 1.5g is diluted with water to 1000g, spray application.
Example of formulations 8
Get 0.12 gram of chitosan oligosaccharide, uniconazole P 0.2g, potassium phosphate 3.5g, is diluted with water to 1000g, spray application.
Example of formulations 9
Get 0.15 gram of chitosan oligosaccharide, dipotassium hydrogen phosphate 3.0g, calcium chloride 1.5g, is diluted with water to 1000g, spray application.
Example of formulations 10
Get 0.15 gram of chitosan oligosaccharide, dipotassium hydrogen phosphate 3.0g, is diluted with water to 1000g, spray application.
Effect embodiment 1:
Select the plants such as apple, mango, grape, lichee, cherry, strawberry, red French beans, tomato, orchid, kaffir lily, by the preparation dilution of example of formulations 1-10, what wherein concentration represented is the valid density of chitosan oligosaccharide, with clear water, processes in contrast respectively.By the solution of above-mentioned preparation according to demand treatment by stages the crop in each vegetative stage, more than 20 strains are at least processed in every processing.The time interval of processing is repeatedly 10 days.Contrast adopts clear water to process, and rinse dosage is identical with the consumption of preparation.In processing plant moment, take at random the different plants organ of chitosan oligosaccharide and compositions-treated, spectrophotometric method is measured the anthocyanidin content of more different plant organs, the detected value of processing with clear water is defined as 100, and the percentage that the value that the value of processing contrasts with clear water compares the results are shown in Table 1.
The anthocyanidin content of the different plants of table 1 after chitosan oligosaccharide and compositions-treated thereof
Figure BDA0000141376530000041
Figure BDA0000141376530000051
No matter by test, estimated and shown, be single or continuous administration chitosan oligosaccharide and chitosan oligosaccharide composition, and plant coloring effect improves.Through spectrophotometric method colorimetric estimation, find (in Table 1), with respect to blank, chitosan oligosaccharide and chitosan oligosaccharide composition different disposal plant, in plant organ, the content of anthocyan all improves, visible, chitosan oligosaccharide and chitosan oligosaccharide composition obviously improve the coloring effect of plant.

Claims (7)

1. one kind for improving the painted chitosan oligosaccharide agriculturally useful compositions of plant, the percentage by weight that contains chitosan oligosaccharide in this agriculturally useful compositions is 0.0015%-0.015%, also containing other compositions, other compositions are one or more in glycine, levulinic acid, glutamic acid, phenyl alanine, TYR, acetylsalicylic acid, Genistein, uniconazole P, and wherein the gross weight of other compositions accounts for the 0.05-5% of composition total weight.
2. composition according to claim 1, wherein to account for the percentage by weight of composition be 0.0025%-0.01% to chitosan oligosaccharide.
3. composition according to claim 1, wherein other compositions are two or more in glycine, levulinic acid, glutamic acid, phenyl alanine, TYR, acetylsalicylic acid, Genistein, uniconazole P.
4. composition according to claim 1, wherein the gross weight of other compositions accounts for the 0.05-1% of composition total weight.
5. the composition described in claim 1-4 any one is for improving the painted purposes of plant.
6. the composition described in claim 1-4 any one is used for improving plant colorize method, it is characterized in that every 7-14 days, in seedling stage, the vegetative period of plant, bloom before and/or bloom after use described agriculturally useful compositions.
7. method according to claim 6, it is characterized in that every 10 days, then use 0-3 time, then the concentration of the concentration of using and front applied once is identical or different.
CN201210058562.2A 2012-03-07 2012-03-07 Chitosan oligosaccharide agricultural composition for improving coloration of plants and application thereof and method by utilizing same to improve coloration of plants Active CN102613198B (en)

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CN104649807A (en) * 2013-11-21 2015-05-27 中国科学院大连化学物理研究所 Chitosan oligosaccharide compound and application of chitosan oligosaccharide compound in relieving albino seedling of graminaceous crops
CN105432343B (en) * 2015-11-11 2018-03-23 长江大学 A kind of application for the method and Genistein for improving ginkgo biloba flavonoid content
CN105985179A (en) * 2015-12-29 2016-10-05 芜湖市创源新材料有限公司 Coloring promotion type special fertilizer for grapes
CN107739250A (en) * 2017-11-16 2018-02-27 潍坊友容实业有限公司 Saline and alkaline region orchard functional form liquid fertilizer and preparation method thereof
CN111018600A (en) * 2019-12-15 2020-04-17 惠州学院 Foliar fertilizer for improving anthocyanin content of litchi peels and preparation and use methods thereof
CN111559937A (en) * 2020-05-21 2020-08-21 潍坊真维农生物工程有限公司 Biological activator for promoting fruit coloring and preparation method and application thereof
CN114437250B (en) * 2022-03-15 2023-07-04 内蒙古农业大学 Chitosan oligosaccharide amino hydroxy propionamide derivative and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101305738A (en) * 2007-05-18 2008-11-19 叶青 Agricultural mixed formulation constituted by chitosan oligosaccharide or the like
CN101462896A (en) * 2008-11-06 2009-06-24 常大勇 Leaf surface calcareous fertilizer
CN102057906A (en) * 2010-12-10 2011-05-18 常大勇 Fruit coloring adjunct and method for promoting fruit coloring with same
CN102144637A (en) * 2010-12-23 2011-08-10 海南正业中农高科股份有限公司 Chitosan oligosaccharide composite with cold-resistant function and application of composite

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101305738A (en) * 2007-05-18 2008-11-19 叶青 Agricultural mixed formulation constituted by chitosan oligosaccharide or the like
CN101462896A (en) * 2008-11-06 2009-06-24 常大勇 Leaf surface calcareous fertilizer
CN102057906A (en) * 2010-12-10 2011-05-18 常大勇 Fruit coloring adjunct and method for promoting fruit coloring with same
CN102144637A (en) * 2010-12-23 2011-08-10 海南正业中农高科股份有限公司 Chitosan oligosaccharide composite with cold-resistant function and application of composite

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
具有农业生物活性壳寡糖的研究进展;刘幸海等;《农药学学报》;20060330;第8卷(第01期);1-7 *
刘幸海等.具有农业生物活性壳寡糖的研究进展.《农药学学报》.2006,第8卷(第01期),1-7.
花青素的生物合成和成色机理及提高其稳定性的途径;顾林等;《食品工业科技》;20071125;第28卷(第11期);240-243 *
顾林等.花青素的生物合成和成色机理及提高其稳定性的途径.《食品工业科技》.2007,第28卷(第11期),240-243.

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