CN105432343B - A kind of application for the method and Genistein for improving ginkgo biloba flavonoid content - Google Patents
A kind of application for the method and Genistein for improving ginkgo biloba flavonoid content Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Abstract
The invention provides a kind of method for improving ginkgo biloba flavonoid content, its step includes：Ginkgo is handled with Genistein.Further, the application present invention also offers Genistein in plant flavonoids content is improved.By applying Genistein to ginkgo, change ginkgo body physiological metaboilic level, promote ginkgo photosynthesis, so as to promote Flavonoids Accumulation, improve ginkgo biloba flavonoid content, improve the medicinal and economic value of ginkgo, and it is easy to operate, cost is cheap.The blade handled through Genistein, flavonoids, anthocyanidin content, soluble sugar content, chlorophyll content significantly improve, activities of antioxidant enzymes, phenylalanine lyase, chalcone synthase and enzyme, namely chalcone isomerase activity are remarkably reinforced, therefore Flavonoid Content is not only improved, also substantially increase the resistance of plant, so as to improve plant products and quality, there is good popularizing application prospect.
Description
Technical field
The invention belongs to agricultural science and technology technical field, more particularly to a kind of method for improving ginkgo biloba flavonoid content and goldspink
The application of different flavine.
Background technology
Ginkgo (Ginkgo biloba L.) originates in China, Ginkgoaceae Ginkgo, deciduous tree, dioecism, is also known as white
Fruit tree, Gong Sunshu.Ginkgo is most ancient Relict Plant in the world at present, there is the title of " living fossil ".Scientist is early in previous generation
The seventies of research of recording has shown that medicinal valency of the ginkgo biloba p.e to the extraordinary therapeutic effect of cardiovascular and cerebrovascular disease, since then ginkgo
Value has become one of focus of international medicinal plants study exploitation by people's extensive concern, nowadays ginkgo.Wherein ginkgo class is yellow
Assimilation compound is the preferred natural drug for treating senile dementia, to hypertension, atherosclerosis, diabetes, angiocarpy
Disease etc. also has very significant therapeutic effect.In addition, flavone compound is distributed widely in plant kingdom, there is many biologies
Function is learned, such as coordinate plant growth, ultraviolet is protected the plants from, antioxidation activity, anti-aging, resists pathogenic bacteria invasion and attack, shadow
Ring the fecundity of plant and the sprouting of pollen.Although ginkgo biloba flavonoid class compound has important pharmacology and physiologically active,
Be low in ginkgo in-vivo content, be not easy to extract, thus improve Ginkgo biloba leaf flavonoids content so as to improve the resistance of ginkgo into
For research emphasis.
Genistein (Genistein, GNT) is also known as genistein or genistein, is day in a kind of soybean body
So existing isoflavone like substance, or the inhibitor of LCK, it is used to study turning for cell G-protein signal
Lead.Siskin isoflavonoid and its glucosides have weak estrogenic activity, have good anticancer, anti-oxidant and suppression hydrogen peroxide life
Into, prevention middle-aged and senile osteoporosis, improve female dimacteric syndrome and angiocardiopathy, increase activities of antioxidant enzymes etc. and make
With being widely used as nutritional food replenisher.
Numerous researchs show that GNT can promote the growth of fowl poultry kind in recent years, improve lactation or egg laying performance, improve breeding
Power and enhancing immunity of organisms, can promote peento fruit color, improve peento sugar-acid ratio, promote Apple coloring and peach fruit
Real coloring, increase grape fruit anthocyanin accumulation etc..But the research for GNT in terms of plant flavonoids, there is not any phase
Close report.
The content of the invention
In view of this, the invention provides it is a kind of improve ginkgo biloba flavonoid content method and Genistein application,
Solve the problems, such as that Folium Ginkgo Flavonoid Content is extremely low, there is provided a kind of easy to operate, cost is low, can efficiently improve ginkgo
Flavonoid Content method.
First aspect present invention provides a kind of method for improving ginkgo biloba flavonoid content, and its step includes：It is different with goldspink
Flavine handles ginkgo.
Second aspect of the present invention provides application of the Genistein in plant flavonoids content is improved.
The beneficial effects of the invention are as follows：1. the present invention is changed raw in ginkgo body by applying Genistein to ginkgo
Metaboilic level is managed, promotes ginkgo photosynthesis, so as to promote Flavonoids Accumulation, ginkgo biloba flavonoid content is improved, improves ginkgo
Medicinal and economic value, and it is easy to operate, cost is cheap.
2. the blade handled through Genistein, flavonoids, anthocyanidin content, soluble sugar content, chlorophyll content show
Writing and improve, activities of antioxidant enzymes, phenylalanine lyase, chalcone synthase and enzyme, namely chalcone isomerase activity are remarkably reinforced, because
This not only improves Flavonoid Content, also substantially increases the resistance of plant, so as to improve plant products and quality, has well
Popularizing application prospect.
Embodiment
First aspect present invention provides a kind of method for improving ginkgo biloba flavonoid content, and its step includes：It is different with goldspink
Flavine handles ginkgo.Genistein is applied to ginkgo, ginkgo body physiological metaboilic level is changed, promotes the photosynthetic work of ginkgo
With so as to promote Flavonoids Accumulation, raising ginkgo biloba flavonoid content, and the anthocyanidin content of plant, soluble sugar content, Ye Lv
Cellulose content significantly improves, and activities of antioxidant enzymes, phenylalanine lyase, chalcone synthase and enzyme, namely chalcone isomerase activity are obvious
Enhancing.
Preferably, the Genistein is formulated as the aqueous solution post processing ginkgo of Genistein, the different Huang of goldspink
Plain concentration is 50-150 μm of ol/L.With increasing for genistein concentration is sprayed in certain limit, the class in Folium Ginkgo is yellow
Ketone content is in rising trend, and downward trend after first rising, 50- is presented in ginkgo leaf anthocyanidin, phenylalanine lyase content
Parameters are preferable in 150 μm of ol/L concentration range, are advantageously metabolized in ginkgo photosynthesis and body physiological.
More preferred, contain solubilizer in the Genistein aqueous solution.The solubilizer can increase the different Huang of goldspink
The solubility of element in aqueous, disperse more uniformly, to be advantageous to absorption of the plant to Genistein.
It is further preferred that the solubilizer is tween, the tween accounts for the percent by volume of the Genistein aqueous solution
For 0.05-0.2%.
Preferably, the method for the processing is in Folium Ginkgo table after ginkgo germinates one month by Genistein spraying
Face.It is now more notable to the effect of ginkgo effect after being germinateed one month for ginkgo using the best opportunity of Genistein.
More preferred, the dosage of the Genistein is 1-5 μm of ol.
Second aspect of the present invention provides application of the Genistein in plant flavonoids content is improved.
Preferably, the plant is ginkgo.
It is more preferred, include the step of the application：After ginkgo germinates one month, Genistein is formulated as gold
Spraying is in Folium Ginkgo surface after the aqueous solution of the different flavine of sparrow, and the genistein concentration is 50-150 μm of ol/L, the gold
Containing the tween that percent by volume is 0.05-0.2% in the different flavine aqueous solution of sparrow.
Below in conjunction with embodiment to a kind of method and the different Huang of goldspink for improving ginkgo biloba flavonoid content provided by the invention
The application of element is further described.
Embodiment 1
1 method of testing
Each assay method is as follows in the present embodiment, the experimental method of unreceipted actual conditions in case study on implementation, according to routine
Condition, or according to the condition proposed by manufacturer.
1.1 ginkgo measuring chlorophyll contents
Chlorophyll a, the measure of content of chlorophyll b are carried out in following manner.Take 3g fresh leafs, punching sampling, grinding, addition
80% acetone extracts, and is measured after constant volume under 663nm and 645nm wavelength, according to C (mgL-1The OD 645+8.04 of)=20.31
OD 663, (OD is light splitting value) calculate chlorophyll concentration.Finally use formula：Y(mg·dm-2)=[concentration (mgL-1) × extraction
Liquid total amount (mL)/[leaf area (dm2) × 100] it is converted into chlorophyll content.3 replications are carried out, are averaged.
The measure of 1.2 anthocyanidin contents
The content of blade anthocyanidin is measured using AAS, 3 replications is carried out, averages.
The measure of 1.3 ginkgo soluble sugar contents
Soluble sugar is determined using anthrone colorimetry, four groups of processing weigh the drying of 10g ginkgo leaves respectively, after taking 1g to dry
Blade is ground into homogenate with 10ml ethanol solutions, after standing 30min, 4500rpm centrifugation 15min, takes supernatant 3ml to 25ml to hold
Measuring bottle adds water constant volume.Then take 1ml dilutions to add 5ml anthrone reagents, boil 10min, extinction is determined in 620nm after cooling
Value.
The measure of 1.4 ginkgo PAL activities
With determined by ultraviolet spectrophotometry ginkgo PAL activity, the fresh Folium Ginkgos of 2.0g are taken, add 3ml
Borate buffer and 0.5g PVP, grinding, 10 000rpm centrifugation 15min, take supernatant.0.5mL Phe solutions are taken to add
0.5ml supernatants.Then 10ml distilled water is added, 30min is heated in 40 DEG C of water-bath, then boils 5min in boiling water and terminates instead
Should, spectrophotometric value is determined under 290nm, a unit of enzyme activity (U) is used as using enzyme amount needed for Change of absorption 0.01.Carry out 3
Secondary replication, averages.
1.5 the measure of ginkgo biloba flavonoid content
General flavone content in determined by ultraviolet spectrophotometry extract, dry, add after weighing ginkgo fresh leaf 1.0g
The immersion of 10ml70% ethanol, filter, in triplicate.Merging filtrate, it is to be measured to be settled to 50ml.It is molten to draw 5ml 100ug/L rutins
Liquid, 30% ethanol 10ml, 0.7ml 5%NaNO2, 0.7ml 10%AlCl are added after mixing3.Add 5ml 40%NaOH after 5min,
30% ethanol constant volume 25ml.After 35 DEG C of water-bath 10min, 510nm wavelength measure absorbance.Using rutin as standard specimen, Flavonoid Content
As a percentage.
The measure of 1.6 ginkgo activities of antioxidant enzymes
Using nitroblue tetrazolium method measure superoxide dismutase (SOD) activity, it is to suppress the 50% of NBT photoreductions
One unit of enzyme activity (U)；Using guaiacol determination of color peroxidase (POD) activity, with interior 470nm per minute
Optical density (D470nm) change 0.1 be a unit of enzyme activity (U)；
Using ultraviolet absorption method measure catalase (CAT) activity, with A in 1min240It is an enzymatic activity to reduce by 0.1
Unit (U)；Using APX in H2O2The principle of ascorbic acid amount reduction is set to determine enzymatic activity, ultraviolet-visible point under the conditions of existing
Change of the absorption photometric value in 3 minutes in every 15 seconds at 290nm is read on light photometer.Calculate interior every milligram of albumen per minute
The ascorbic acid amount of conversion, it is a unit of enzyme activity (U).
2 experimentations and result
2.1 experimentation
1) acquisition of vegetable material
The test material of the present invention is 1 year raw ginkgo seedling, and kind is " family Buddha's hand ".Cultivation matrix is river sand and humic
Soil presses 1：2 proportioning mixed-matrixes, flowerpot (diameter 25cm, high 35cm), per one plant of basin, are placed in greenhouse, illumination 12h/d, relatively
Humidity 60-70%.Poured per basin with 100ml liquid fertilizer (containing 0.36g N, 0.12g P, 0.14g K) weekly.
2) experiment process
The present invention sets 0 (control group), 50,100 and 150 μm of ol/L, tetra- concentration when ginkgo seedling grows four blades
The GNT aqueous solution of gradient, and contain the tween that volume ratio is 0.1% in the aqueous solution.Ginkgo seedling is sprayed, each processing sets 3 small
Area, each cell set 3 repetitions, choose growing way 36 plants of ginkgo seedlings of identical altogether.Monthly, each 20ml, continuously sprays three
Month.When spraying, all blades and its positive and negative will be sprayed onto.GNT starts sampling test after having handled 4 weeks.
2.2 experimental result
2.2.1GNT to the influence of chlorophyll content
Using testing procedure described in the part of the present embodiment 1.1, influences of the GNT measured to chlorophyll content the results are shown in Table 1.
Influences of the GNT of table 1 to ginkgo leaf chlorophyll content
Note：Different letters are represented in p in same row<5% horizontal upper significant difference.
As shown in Table 1,100,150 μm of ol/L GNT processing can improve the content of chlorophyll a, chlorophyll b.100μmol/
Chlorophyll a, the content of chlorophyll b of L GNT processing are peak, and respectively 1.386,0.647mg/g FW, it is significantly high respectively
In control group 45.58%, 34.51%, and P<0.05 has statistical significance.Chlorophyll a+b amplitude of variation and chlorophyll a,
The change of the content of chlorophyll b is consistent.The content of chlorophyll a/b is all higher than 1, and there was no significant difference.
2.2.2 influences of the GNT to soluble sugar content
Using testing procedure described in the part of the present embodiment 1.3, influences of the GNT measured to soluble sugar content the results are shown in Table
2。
The GNT of table 2 is to ginkgo leaf soluble sugar, the titratable influence for calculating content
Note：Different letters are represented in p in same row<5% horizontal upper significant difference.
Soluble sugar content measurement result shows shown in table 2, and the soluble sugar of 50,100,150 μm of ol/L GNT processing contains
Amount is above control group, is not higher by than control component：20.04%th, 10.11%, 15.27%.Show to spray GNT to Folium Ginkgo
Accumulation carbohydrate has certain facilitation.
2.2.3 influences of the GNT to Flavonoid Content and anthocyanidin content
Using testing procedure described in the present embodiment 1.2,1.5 parts, the GNT measured is to Flavonoid Content and anthocyanidin content
Influence the results are shown in Table 3.
Influences of the GNT of table 3 to Ginkgo biloba leaf flavonoids, anthocyanidin content
Note：Different letters are represented in p in same project<5% horizontal upper significant difference.
Result is visible as shown in Table 3, and with increasing for GNT concentration is sprayed, the Flavonoid Content in Folium Ginkgo is in rising
Trend.It is 159.74mg to spray 150 μm of ol/L GNT and measure Flavonoid Content average value.100.g-1FW, significantly it is higher by control group
20.88%, and P<0.05 has statistical significance, it follows that conclusion, is advantageous to ginkgo leaf class Huang to ginkgo foliage-spray GNT
The increase of ketone content.100th, the anthocyanidin content of 150 μm of ol/L GNT processing is respectively 0.395,0.350mg 100g-1FW, than
Control group is higher by 27.01%, 12.54% respectively.GNT is advantageous to ginkgo leaf anthocyanidin in certain concentration range and contained as can be seen here
The increase of amount.2.2.4GNT to the influence of phenylalanine lyase, chalcone synthase and enzyme, namely chalcone isomerase activity
Using testing procedure described in the part of the present embodiment 1.4, the GNT measured synthesizes to phenylalanine lyase, chalcone
The influence of enzyme and enzyme, namely chalcone isomerase activity the results are shown in Table 4.
Influences of the GNT of table 4 to ginkgo leaf phenylalanine lyase, chalcone synthase and enzyme, namely chalcone isomerase activity
Note：Different letters are represented in p in same row<5% horizontal upper significant difference.
From table 4, with spraying increasing for GNT concentration, the phenylalanine lyase content in Folium Ginkgo is on first
Downward trend after rising.The phenylalanine lyase content highest of 100 μm of ol/L GNT processing, is 344.592U mg-1, it is significantly super
Go out control group (267.15) 28.99% and P<0.05 has statistical significance.The phenylalanine solution of 150 μm of ol/L GNT processing
Ammonia enzymatic activity is obviously reduced, and the GNT with spraying 100 μm of ol/L reduces 3.44%.As a result show, in the range of finite concentration,
The activity of ginkgo leaf phenylalanine lyase can be improved to ginkgo foliage-spray GNT.And with spraying increasing for GNT concentration,
Chalcone synthase, enzyme, namely chalcone isomerase content in Folium Ginkgo is in rising trend.
2.2.5 influences of the GNT to activities of antioxidant enzymes
Using testing procedure described in the part of the present embodiment 1.6, influences of the GNT measured to activities of antioxidant enzymes the results are shown in Table
5。
Influences of the GNT of table 5 to ginkgo leaf activities of antioxidant enzymes
Note：Different letters are represented in p in same row<5% horizontal upper significant difference.
From table 5, with spraying increasing for GNT concentration, superoxide dismutase SOD and peroxidating in Folium Ginkgo
Hydrogen enzyme CAT activity is in rising trend.It is 4.37U/mg to spray 150 μm of ol/L GNT and measure SOD activity average value, is significantly higher by pair
According to 89.17% (P of group<0.05) it is 84.42U/mg, to measure CAT activity average value, is significantly higher by the (P of control group 33.76%<
0.05), it follows that conclusion, be advantageous to improve SOD and CAT activity to ginkgo foliage-spray GNT.With spraying GNT concentration
Increase, ascorbic acid peroxidase APX and peroxidase POD activity first rises and declined afterwards in Folium Ginkgo.100μ
The active highest of mol/L GNT processing, is 70.46U/mg and 1.61U/mg, significantly exceeds control group 54.17% and 75.0%
(P<0.05).APX the and POD activity of 150 μm of ol/L GNT processing is obviously reduced, and the GNT with spraying 100 μm of ol/L is reduced
3.0% and 8.69%.As a result show, in the range of finite concentration, ginkgo foliage-spray GNT can be improved ginkgo leaf APX and
The activity of POD activity.
Embodiment 2
1 method of testing
The present embodiment used test method is identical with implementing 1.
2 experimentations and result
2.1 experimentation
1) acquisition of vegetable material
The test material of the present invention is 1 year raw ginkgo seedling, and kind is " family Buddha's hand ".Cultivation matrix is river sand and humic
Soil presses 1：2 proportioning mixed-matrixes, flowerpot (diameter 25cm, high 35cm), per one plant of basin, are placed in greenhouse, illumination 12h/d, relatively
Humidity 60-70%.Poured per basin with 100ml liquid fertilizer (containing 0.36g N, 0.12g P, 0.14g K) weekly.
3) experiment process
The present invention sets 0 (control), 50,100 and 150 μm of ol/L, tetra- concentration ladders when ginkgo seedling grows four blades
The GNT aqueous solution sprinkling ginkgo seedling of degree, and contain the tween that volume ratio is 0.2% in the aqueous solution.Each processing sets 3 cells,
Each cell sets 3 repetitions, chooses growing way 36 plants of ginkgo seedlings of identical altogether.Monthly, each 20ml, continuously sprays March.
When spraying, all blades and its positive and negative will be sprayed onto.
GNT starts sampling test after having handled 4 weeks.
2.2 experimental result
2.2.1 influences of the GNT to Flavonoid Content and anthocyanidin content
Using method of testing described in the part of the present embodiment 1, influences of the GNT measured to Flavonoid Content and anthocyanidin content
It the results are shown in Table 6.
Influences of the GNT of table 6 to Ginkgo biloba leaf flavonoids, anthocyanidin content
Note：Different letters are represented in p in same row<5% horizontal upper significant difference.
From table 6, with increasing for GNT concentration is sprayed, the Flavonoid Content in Folium Ginkgo is in rising trend.Spray
It is 165.57mg that 150 μm of ol/L GNT, which measure Flavonoid Content average value,.100.g-1FW, significantly it is higher by (the P of control group 30.14%<
0.05), it follows that conclusion, be advantageous to the increase of Ginkgo biloba leaf flavonoids content to ginkgo foliage-spray GNT.100、150μmol/
The anthocyanidin content of L GNT processing is respectively 0.409,0.428mg 100g-1FW, be not higher by 37.25% than control component,
43.62%.GNT is advantageous to the increase of ginkgo leaf anthocyanidin content in certain concentration range as can be seen here.
2.2.2 influences of the GNT to phenylalanine lyase, chalcone synthase and enzyme, namely chalcone isomerase activity
Using method of testing described in the part of the present embodiment 1, the GNT measured is to phenylalanine lyase, chalcone synthase
And the influence of enzyme, namely chalcone isomerase activity the results are shown in Table 7.
Influences of the GNT of table 7 to ginkgo leaf phenylalanine lyase, chalcone synthase and enzyme, namely chalcone isomerase activity
Note：Different letters are represented in p in same row<5% horizontal upper significant difference.
From table 7, with spraying increasing for GNT concentration, the phenylalanine lyase content in Folium Ginkgo is on first
Downward trend after rising.The phenylalanine lyase content highest of 100 μm of ol/L GNT processing, is 350.112U/mg, significantly super
Go out (the P of control group 35.07%<0.05).The PAL activity of 150 μm of ol/L GNT processing is obviously reduced, with spraying
100 μm of ol/L GNT reduces 6.06%.The activity of chalcone synthase and enzyme, namely chalcone isomerase is with the increase of GNT concentration
And increase, the chalcone synthase and enzyme, namely chalcone isomerase activity of 150 μm of ol/L GNT processing are higher than control group respectively
109.15% and 118.17% (P<0.05).As a result show, in the range of finite concentration, ginkgo foliage-spray GNT can be carried
The activity of high ginkgo leaf phenylalanine lyase, chalcone synthase and enzyme, namely chalcone isomerase.
2.2.3 influences of the GNT to activities of antioxidant enzymes
Using method of testing described in the part of the present embodiment 1, influences of the GNT measured to activities of antioxidant enzymes the results are shown in Table 8.
Influences of the GNT of table 8 to ginkgo leaf activities of antioxidant enzymes
Note：Different letters are represented in p in same row<5% horizontal upper significant difference.
From table 8, with increasing for GNT concentration is sprayed, SOD the and CAT activity in Folium Ginkgo is in rising trend.Spray
It is 4.19U/mg to apply 150 μm of ol/L GNT and measure SOD activity average value, is significantly higher by the (P of control group 90.76%<0.05), measure
CAT activity average value is 86.15U/mg, is significantly higher by the (P of control group 41.38%<0.05), it follows that conclusion, to ginkgo leaf
Face sprays the activity that GNT is advantageous to improve SOD and CAT.With spraying increasing for GNT concentration, APX and POD in Folium Ginkgo
Activity first rises to be declined afterwards.The active highest of 100 μm of ol/L GNT processing, is 69.912U/mg and 1.79U/mg, significantly exceeds
(the P of control group 70.96% and 110.81%<0.05).APX the and POD activity of 150 μm of ol/L GNT processing is obviously reduced, with spray
Applying 100 μm of ol/L GNT reduces 4.25% and 11.2%.As a result show, in the range of finite concentration, to ginkgo foliage-spray
GNT can improve ginkgo leaf APX and POD activity.
From embodiment 1, the measurement result of embodiment 2, GNT processing, which can stablize, improves flavonoids, leaf in ginkgo leaf
Green element, soluble sugar, the content of anthocyanidin and phenylalanine lyase and the activity of antioxidase.By analyze chlorophyll,
Soluble sugar, the content of anthocyanidin and phenylalanine lyase and the activity of antioxidase are related between Flavonoid Content
Property, ginkgo active component class can be improved by promoting ginkgo photosynthesis by further demonstrating Genistein (GNT)
Flavones content.
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent substitution and improvements made etc., it should be included in the scope of the protection.
Claims (8)
1. a kind of method for improving ginkgo biloba flavonoid content, its step include：Ginkgo is handled with Genistein.
2. the method for ginkgo biloba flavonoid content is improved as claimed in claim 1, it is characterised in that：The Genistein is prepared
Ginkgo is post-processed for the aqueous solution of Genistein, the genistein concentration is 50-150 μm of ol/L.
3. the method for ginkgo biloba flavonoid content is improved as claimed in claim 2, it is characterised in that：The Genistein is water-soluble
Contain solubilizer in liquid.
4. the method for ginkgo biloba flavonoid content is improved as claimed in claim 3, it is characterised in that：The solubilizer is tween,
The percent by volume that the tween accounts for the Genistein aqueous solution is 0.05-0.2%.
5. the method for ginkgo biloba flavonoid content is improved as claimed in claim 1, it is characterised in that：The method of the processing be
Ginkgo germinate one month after by Genistein spraying in Folium Ginkgo surface.
6. the method for the raising ginkgo biloba flavonoid content as described in any one of claim 1 to 5 claim, it is characterised in that：
The dosage of the Genistein is 1-5 μm of ol/ strain.
7. application of the Genistein in plant flavonoids content is improved, the plant are ginkgo.
8. application of the Genistein as claimed in claim 7 in plant flavonoids content is improved, it is characterised in that：It is described
Using the step of include：After ginkgo germinates one month, Genistein is formulated as to spraying after the aqueous solution of Genistein
In Folium Ginkgo surface, the genistein concentration is 50-150 μm of ol/L, contains volume in the Genistein aqueous solution
Percentage is 0.05-0.2% tween.
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| CN107548831A (en) * | 2017-08-01 | 2018-01-09 | 六安玫瑰红茶品有限公司 | A kind of mulberry tree management method for improving flavones in mulberry leaves content |
| CN108012698A (en) * | 2017-12-06 | 2018-05-11 | 中国药科大学 | A kind of method for simulating biological stress reaction induction ginkgo Rapid Accumulation ginkgolides |
| CN111543235A (en) * | 2020-05-11 | 2020-08-18 | 扬州大学 | Method for improving total flavonoids in ginkgo leaves |
| CN117625422A (en) * | 2024-01-26 | 2024-03-01 | 龙牌食品股份有限公司 | Pichia burton strain L9-1 and application thereof |
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| CN103314093A (en) * | 2010-10-29 | 2013-09-18 | 施韦特-莫迪国际公司 | Method for producing articles of plant origin impregnated with a liquid plant substance |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1513986A (en) * | 2003-08-08 | 2004-07-21 | 浙江省农业科学院 | Gene engineering method for raising plant useful secondary substance content |
| WO2012006750A1 (en) * | 2010-07-12 | 2012-01-19 | Frutarom Switzerland Ltd. | Nutraceutical composition obtained from fungus-challenged soy seedlings |
| CN103314093A (en) * | 2010-10-29 | 2013-09-18 | 施韦特-莫迪国际公司 | Method for producing articles of plant origin impregnated with a liquid plant substance |
| CN102318486A (en) * | 2011-08-09 | 2012-01-18 | 湖南麓山天然植物制药有限公司 | GAP (good agricultural practices) cultivation technique for leaf-harvesting ginkgo garden with red soil |
| CN102613198A (en) * | 2012-03-07 | 2012-08-01 | 海南正业中农高科股份有限公司 | Chitosan oligosaccharide agricultural composition for improving coloration of plants and application thereof and method by utilizing same to improve coloration of plants |
| CN102939846A (en) * | 2012-11-28 | 2013-02-27 | 西安福安创意咨询有限责任公司 | Planting method for producing ginkgo leaf agricultural products |
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