CN108207960A - Application of the external source epiphysin to Strawberry seedlings Cd stress relieving effect - Google Patents
Application of the external source epiphysin to Strawberry seedlings Cd stress relieving effect Download PDFInfo
- Publication number
- CN108207960A CN108207960A CN201810008533.2A CN201810008533A CN108207960A CN 108207960 A CN108207960 A CN 108207960A CN 201810008533 A CN201810008533 A CN 201810008533A CN 108207960 A CN108207960 A CN 108207960A
- Authority
- CN
- China
- Prior art keywords
- strawberry
- stress
- epiphysin
- improved
- under
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Abstract
The invention discloses a kind of application of external source epiphysin to Strawberry seedlings Cd stress relieving effect.Application of the present invention protection epiphysin in the damage of strawberry Cd stress is alleviated.The present invention is using wonderful fragrant No. 7 strawberries as test material, study the blade injury of strawberry under epiphysin and Cd stress, Antioxidant Enzyme Systems, cell membrane system relationship, the mechanism of action that epiphysin resists heavy metal stress in plant is inquired into, foundation is provided for its safety in production in strawberry.
Description
Technical field
The present invention relates to a kind of application of external source epiphysin to Strawberry seedlings Cd stress relieving effect.
Background technology
Cadmium is the nonessential nutrient of plant.Low concentration cadmium plays plant growth certain facilitation, but high concentration
Cadmium have larger toxic action to plant.With the development of industry, industrial wastewater, pollutant enter soil pollution environment, I
The problem of state's Cadmium Pollution in Soils, is serious.Research shows that excessive cadmium can also make plant generate stress, inhibit chlorophyll synthesis and
Photosynthetic progress, plant there is also the symptoms such as growth retardation, plant are short and small, it is green to move back, yield declines under Cd stress, high concentration
Cadmium can destroy the active oxygen metabolism balance in plant, and the accumulation increase of active oxygen leads to Lipid peroxidation metabolism, anti-in plant
The activity of oxidizing ferment reduces, and strawberry is one of important fruit in China, ends 2015, China's strawberry cultivating area is up to 220
Ten thousand mu.Strawberry is more sensitive to the injury of Cd stress, research shows that, the growth of Strawberry Leaves and anti-oxidant enzyme activity under Cd stress
Property be suppressed, the fruit quality of strawberry declines under Cd stress, and cadmium accumulates the health for also influencing whether the mankind in fruit.
Therefore it is most important to the injury of strawberry to alleviate Cd stress.
Epiphysin (melatonin, abbreviation MT) chemical name is n-acetyl-5-methoxytryptamine, is a kind of indoles
Compound is prevalent in animal body.Recent study finds that epiphysin is also widely present in higher plant body.Study table
Bright, epiphysin has various physiological functions in plant, can adjust photoperiod, coordinate plant growth development.
Invention content
The object of the present invention is to provide a kind of application of external source epiphysin to Strawberry seedlings Cd stress relieving effect.
The present invention provides application of the epiphysin in the damage of strawberry Cd stress is alleviated.
The present invention also protects the application of epiphysin, at least one of following (a1)-(a5)：
(a1) strawberry Determination of Chlorophyll content under Cd stress is improved；
(a2) activities of antioxidant enzymes in strawberry is improved under Cd stress；
(a3) mda content in strawberry is reduced under Cd stress；
(a4) soluble protein content in strawberry is improved under Cd stress；
(a5) strawberry Cd stress resistance is improved.
The present invention also protects a kind of product, and active constituent is epiphysin；The purposes of the product is following (b1)-(b6)
At least one of：
(b1) alleviate the damage of strawberry Cd stress；
(b2) strawberry Determination of Chlorophyll content under Cd stress is improved；
(b3) activities of antioxidant enzymes in strawberry is improved under Cd stress；
(b4) mda content in strawberry is reduced under Cd stress；
(b5) soluble protein content in strawberry is improved under Cd stress；
(b6) strawberry Cd stress resistance is improved.
The present invention also application of the protection epiphysin in product is produced；The purposes of the product is in following (b1)-(b6)
At least one：
(b1) alleviate the damage of strawberry Cd stress；
(b2) strawberry Determination of Chlorophyll content under Cd stress is improved；
(b3) activities of antioxidant enzymes in strawberry is improved under Cd stress；
(b4) mda content in strawberry is reduced under Cd stress；
(b5) soluble protein content in strawberry is improved under Cd stress；
(b6) strawberry Cd stress resistance is improved.
The present invention also protects a kind of strawberry breeding method, includes the following steps：Using a concentration of 0.1-200 μm of olL-1's
Epiphysin carries out foliage-spray to the strawberry.
Described method includes following steps：Using a concentration of 200 μm of olL-1Epiphysin to the strawberry carry out blade face
It sprays.
The present invention also protects a kind of method for alleviating the damage of strawberry Cd stress, includes the following steps：Using concentration 0.1-200
μmol·L-1Epiphysin to the strawberry carry out foliage-spray.
Described method includes following steps：Using a concentration of 200 μm of olL-1Epiphysin to the strawberry carry out blade face
It sprays.
In more than people's any description above method, the strawberry of the strawberry concretely Seedling Stage.
Application of the present invention also protection epiphysin in Cadmium resistance coerces strawberry selection and breeding.
Application of the method for the present invention also protection any description above in Cadmium resistance coerces strawberry selection and breeding.
Any description above strawberry can be wonderful fragrant No. 7 strawberries.
The present invention is using wonderful fragrant No. 7 strawberries as test material, research epiphysin and blade injury, the antioxygen of strawberry under Cd stress
Change enzyme system, the relationship of cell membrane system, inquire into epiphysin and the mechanism of action of heavy metal stress is resisted in plant, be it in strawberry
Safety in production provide foundation.
Description of the drawings
Fig. 1 is variation of the various concentration MT root irrigations to the Cd stress Strawberry Leaves Visible injury of 10 days.
Fig. 2 is MDA assay results.
Fig. 3 is SOD determination of activity results.
Fig. 4 is POD Activity determination results.
Fig. 5 is CAT Activity determination results.
Fig. 6 is soluble protein content measurement result.
Specific embodiment
Following embodiment facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiments
Method is conventional method unless otherwise specified.Test material used in following embodiments is certainly unless otherwise specified
What routine biochemistry reagent shop was commercially available.Quantitative test in following embodiment is respectively provided with three repeated experiments, as a result makes even
Mean value.
Using wonderful fragrant No. 7 strawberries as test material in embodiment.Wonderful fragrant No. 7 strawberries：Bibliography：Luo Jingjing, Zhang Ya
Fly, wonderful fragrant No. 7 of Peng Futian ' ' strawberry stem tip culture and its effect assessment [J] fruit trees, 2016 (5):57-60.；The public can
To be obtained from Shandong Agricultural University.
Epiphysin (MT)：It buys in sigma companies, article No. M5250.
The suitable epiphysin concentration and Determination of Physiological And Biochemical Indices of embodiment 1, Strawberry seedlings Cadmium resistance stress
First, test process
Long wonderful fragrant No. 7 strawberries to 5-7 piece tender leafs are taken, various concentration is contained to strawberry foliage-spray at the 2nd, 4,6 day and is taken off
Melanocyte (0.1 μm of olL-1、10μmol·L-1、50μmol·L-1、100μmol·L-1、150μmol·L-1With 200 μm of olL-1) water, Application is dripped with blade to be advisable, and control group (CK), which pours, does not contain the water of epiphysin, and each concentration handles 3 plants,
It sprays 3 times daily；It is applied in the 8th day pouring root and contains 1mmol/l CdCl21/2Hoagland nutrient solution, carry out Cd stress
Processing, Cd stress treated the 0th day, the 5th day and the 10th day receive sample be detected.
2nd, physiological index determining
To step 1 processing each group Strawberry Leaves carry out physical signs (chlorophyll content, superoxide dismutase activity,
Peroxidase activity, catalase activity, mda content and soluble protein content) it measures.
Chlorophyll content is measured using acetone method；Superoxide dismutase (SOD) activity is surveyed using superoxide dismutase
It is fixed；The measure of peroxidase (POD) activity is measured using guaiacol method；Catalase (CAT) activity uses ultraviolet point
Light photometry measures；Malonaldehyde (MDA) content uses thiobarbituricacidα- (TBA) determination of color；Soluble protein content is adopted
It is measured with dying method with coomassie brilliant blue.
3rd, measurement result
Various concentration MT root irrigations are shown in Fig. 1 to the variation of the Cd stress Strawberry Leaves Visible injury of 10 days.Each group leaf is green
Cellulose content measurement result is shown in Table 1.Each group MDA assay results are shown in Fig. 2.Each group SOD determination of activity results are shown in Fig. 3.Each group POD
Activity determination result is shown in Fig. 4.Each group CAT Activity determination results are shown in Fig. 5.Each group soluble protein content measurement result is shown in Fig. 6.
Influences of the external source MT to Strawberry Leaves chlorophyll content under 1 Cd stress of table
The above results show that external source epiphysin can significantly improve the chlorophyll content of the Strawberry Leaves under Cd stress；Increase
The activity of SOD (superoxide dismutase), POD (peroxidase), CAT (catalase) by force；Reduce malonaldehyde (MDA)
Content；Soluble protein content is improved, so as to alleviate the adverse reaction that cadmium comes vegetational zone, enhances resistance.MT pouring roots are applied
When Valid concentration be 0.1-200 μm of olL-1, optium concentration is 200 μm of olL-1。
Claims (10)
1. application of the epiphysin in the damage of strawberry Cd stress is alleviated.
2. the application of epiphysin, at least one of following (a1)-(a5)：
(a1) strawberry Determination of Chlorophyll content under Cd stress is improved；
(a2) activities of antioxidant enzymes in strawberry is improved under Cd stress；
(a3) mda content in strawberry is reduced under Cd stress；
(a4) soluble protein content in strawberry is improved under Cd stress；
(a5) strawberry Cd stress resistance is improved.
3. a kind of product, active constituent is epiphysin；The purposes of the product is at least one of following (b1)-(b6)：
(b1) alleviate the damage of strawberry Cd stress；
(b2) strawberry Determination of Chlorophyll content under Cd stress is improved；
(b3) activities of antioxidant enzymes in strawberry is improved under Cd stress；
(b4) mda content in strawberry is reduced under Cd stress；
(b5) soluble protein content in strawberry is improved under Cd stress；
(b6) strawberry Cd stress resistance is improved.
4. application of the epiphysin in product is produced；The purposes of the product is at least one of following (b1)-(b6)：
(b1) alleviate the damage of strawberry Cd stress；
(b2) strawberry Determination of Chlorophyll content under Cd stress is improved；
(b3) activities of antioxidant enzymes in strawberry is improved under Cd stress；
(b4) mda content in strawberry is reduced under Cd stress；
(b5) soluble protein content in strawberry is improved under Cd stress；
(b6) strawberry Cd stress resistance is improved.
5. a kind of strawberry breeding method, includes the following steps：Using a concentration of 0.1-200 μm of olL-1Epiphysin to the grass
The certain kind of berries carries out foliage-spray.
6. method as claimed in claim 6, it is characterised in that：Using a concentration of 200 μm of olL-1Epiphysin to the grass
The certain kind of berries carries out foliage-spray.
7. a kind of method for alleviating the damage of strawberry Cd stress, includes the following steps：Using 0.1-200 μm of olL of concentration-1Take off it is black
Element carries out foliage-spray to the strawberry.
8. the method for claim 7, it is characterised in that：Using a concentration of 200 μm of olL-1Epiphysin to the grass
The certain kind of berries carries out foliage-spray.
9. application of the epiphysin in Cadmium resistance coerces strawberry selection and breeding.
10. application of any methods of claim 5-8 in Cadmium resistance coerces strawberry selection and breeding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810008533.2A CN108207960A (en) | 2018-01-04 | 2018-01-04 | Application of the external source epiphysin to Strawberry seedlings Cd stress relieving effect |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810008533.2A CN108207960A (en) | 2018-01-04 | 2018-01-04 | Application of the external source epiphysin to Strawberry seedlings Cd stress relieving effect |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108207960A true CN108207960A (en) | 2018-06-29 |
Family
ID=62642808
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810008533.2A Pending CN108207960A (en) | 2018-01-04 | 2018-01-04 | Application of the external source epiphysin to Strawberry seedlings Cd stress relieving effect |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108207960A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108660104A (en) * | 2018-04-27 | 2018-10-16 | 西北大学 | Application of the external source epiphysin in improving Chlamydomonas reinhardtii saline-alkaline tolerance |
| CN110679364A (en) * | 2019-09-27 | 2020-01-14 | 昆明理工大学 | Method for improving leaf disease resistance of panax notoginseng and application of method |
| CN110839618A (en) * | 2019-11-18 | 2020-02-28 | 浙江中环农业开发有限公司 | Nano protective agent for improving stress resistance of crops and preparation method thereof |
| CN114467541A (en) * | 2022-01-26 | 2022-05-13 | 西北农林科技大学 | Application of dopamine in improving heavy metal stress resistance of plants |
| CN114711044A (en) * | 2022-03-31 | 2022-07-08 | 江西农业大学 | Application of puerarin in relieving cadmium stress of plants and/or improving plant yield |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080305096A1 (en) * | 2007-06-07 | 2008-12-11 | Unicity International, Inc. | Method and composition for providing controlled delivery of biologically active substances |
| CN104604864A (en) * | 2015-02-13 | 2015-05-13 | 中国农业大学 | Melatonin solution capable of improving anti-stress capability of plant |
| CN104823738B (en) * | 2015-04-13 | 2017-06-16 | 浙江大学 | Epiphysin is reducing the application in plant in Cd accumulation |
| CN107041181A (en) * | 2017-04-14 | 2017-08-15 | 四川农业大学 | It is a kind of to promote pea seedling growth, the method for reduction Cd accumulation |
| CN107278652A (en) * | 2017-06-21 | 2017-10-24 | 四川农业大学 | A kind of method for significantly reducing radish Cd accumulation and promoting it to grow |
| CN107278651A (en) * | 2017-06-21 | 2017-10-24 | 四川农业大学 | A kind of method for significantly reducing root-mustard Cd accumulation and promoting it to grow |
-
2018
- 2018-01-04 CN CN201810008533.2A patent/CN108207960A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080305096A1 (en) * | 2007-06-07 | 2008-12-11 | Unicity International, Inc. | Method and composition for providing controlled delivery of biologically active substances |
| CN104604864A (en) * | 2015-02-13 | 2015-05-13 | 中国农业大学 | Melatonin solution capable of improving anti-stress capability of plant |
| CN104823738B (en) * | 2015-04-13 | 2017-06-16 | 浙江大学 | Epiphysin is reducing the application in plant in Cd accumulation |
| CN107041181A (en) * | 2017-04-14 | 2017-08-15 | 四川农业大学 | It is a kind of to promote pea seedling growth, the method for reduction Cd accumulation |
| CN107278652A (en) * | 2017-06-21 | 2017-10-24 | 四川农业大学 | A kind of method for significantly reducing radish Cd accumulation and promoting it to grow |
| CN107278651A (en) * | 2017-06-21 | 2017-10-24 | 四川农业大学 | A kind of method for significantly reducing root-mustard Cd accumulation and promoting it to grow |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108660104A (en) * | 2018-04-27 | 2018-10-16 | 西北大学 | Application of the external source epiphysin in improving Chlamydomonas reinhardtii saline-alkaline tolerance |
| CN110679364A (en) * | 2019-09-27 | 2020-01-14 | 昆明理工大学 | Method for improving leaf disease resistance of panax notoginseng and application of method |
| CN110679364B (en) * | 2019-09-27 | 2022-03-25 | 昆明理工大学 | Method for improving leaf disease resistance of panax notoginseng and application of method |
| CN110839618A (en) * | 2019-11-18 | 2020-02-28 | 浙江中环农业开发有限公司 | Nano protective agent for improving stress resistance of crops and preparation method thereof |
| CN110839618B (en) * | 2019-11-18 | 2022-09-13 | 浙江中环农业开发有限公司 | Nano protective agent for improving stress resistance of crops and preparation method thereof |
| CN114467541A (en) * | 2022-01-26 | 2022-05-13 | 西北农林科技大学 | Application of dopamine in improving heavy metal stress resistance of plants |
| CN114711044A (en) * | 2022-03-31 | 2022-07-08 | 江西农业大学 | Application of puerarin in relieving cadmium stress of plants and/or improving plant yield |
| CN114711044B (en) * | 2022-03-31 | 2023-03-07 | 江西农业大学 | Application of puerarin in relieving cadmium stress of plants and/or improving plant yield |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108207960A (en) | Application of the external source epiphysin to Strawberry seedlings Cd stress relieving effect | |
| Nigam et al. | Protective role of exogenously supplied salicylic acid and PGPB (Stenotrophomonas sp.) on spinach and soybean cultivars grown under salt stress | |
| Egert et al. | Influence of drought on some physiological parameters symptomatic for oxidative stress in leaves of chives (Allium schoenoprasum) | |
| Sorkheh et al. | Salt stress induction of some key antioxidant enzymes and metabolites in eight Iranian wild almond species | |
| Jaleel et al. | Antioxidant potentials and ajmalicine accumulation in Catharanthus roseus after treatment with giberellic acid | |
| Baque et al. | Production of biomass and bioactive compounds by adventitious root suspension cultures of Morinda citrifolia (L.) in a liquid-phase airlift balloon-type bioreactor | |
| Wada et al. | Involvement of peroxidase in differential sensitivity to γ-radiation in seedlings of two Nicotiana species | |
| Aganchich et al. | Partial root zone drying: regulation of photosynthetic limitations and antioxidant enzymatic activities in young olive (Olea europaea) saplings | |
| Qi-Hua et al. | Effects of elevated air temperature on physiological characteristics of flag leaves and grain yield in rice | |
| Gaburro et al. | Physiological variables related to photosynthesis are more plastic than the morphological and biochemistry in non-pioneer tropical trees under contrasting irradiance | |
| Xiang et al. | Effect of drought stress on yield, chlorophyll contents and photosynthesis in tartary buckwheat (Fagopyrum tataricum) | |
| Asadi Karam et al. | Foliar spray of triacontanol improves growth by alleviating oxidative damage in coriander under salinity | |
| Ahmad et al. | Differential pH-induced biosynthesis of steviol glycosides and biochemical parameters in submerge root cultures of Stevia rebaudiana (Bert.) | |
| Farahbakhsh et al. | Physiological response of henna (Lawsonia inermise L.) to salicylic acid and salinity | |
| Liao et al. | Antioxidant enzyme activity and growth responses of Huangguogan citrus cultivar to nitrogen supplementation | |
| Vijayalakshmi et al. | Salt stress induced modulations in growth, compatible solutes and antioxidant enzymes response in two cultivars of safflower (Carthamus tinctorius L. cultivar TSF1 and cultivar SM) differing in salt tolerance | |
| Ramadan et al. | The combined effect of water deficit stress and TiO2 nanoparticles on cell membrane and antioxidant enzymes in Helianthus annuus L. | |
| CN105432343B (en) | A kind of application for the method and Genistein for improving ginkgo biloba flavonoid content | |
| Chakrabarty et al. | The dynamics of nutrient utilization and growth of apple root stock ‘M9 EMLA’in temporary versus continuous immersion bioreactors | |
| Ivanishchev et al. | Accumulation of nickel ions in seedlings of Vicia sativa L. and manifestations of oxidative stress | |
| Pandey et al. | Analysis of biochemical responses in Vigna mungo varieties subjected to drought stress and possible amelioration | |
| Lin et al. | Antioxidant system of tea (Camellia sinensis) leaves in response to phosphorus supply | |
| Vashishth et al. | Allelopathic effect of Callistemon viminalis’s leaves extract on weeds, soil features, and growth performance of wheat and chickpea plants | |
| Ma et al. | Contrasting growth, physiological and gene expression responses of Clematis crassifolia and Clematis cadmia to different irradiance conditions | |
| Chen et al. | Physiological responses of Agriophyllum squarrosum and Setaria viridis to drought and re-watering |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180629 |