CN107278651A - A kind of method for significantly reducing root-mustard Cd accumulation and promoting it to grow - Google Patents
A kind of method for significantly reducing root-mustard Cd accumulation and promoting it to grow Download PDFInfo
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- CN107278651A CN107278651A CN201710474088.4A CN201710474088A CN107278651A CN 107278651 A CN107278651 A CN 107278651A CN 201710474088 A CN201710474088 A CN 201710474088A CN 107278651 A CN107278651 A CN 107278651A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
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Abstract
The invention provides a kind of method for significantly reducing root-mustard Cd accumulation and promoting it to grow, it includes step：When root-mustard seedling grows 3~4 true leaves, foliage-spray is carried out using the epiphysin that concentration is 50~200 μm of olL 1, the amount of spraying is dripped for degree with blade face.The present invention can significantly reduce the cadmium content in root-mustard；Significantly improve the Contents of Photosynthetic Pigments, activities of antioxidant enzymes and biomass of root-mustard；It is effective to alleviate the inhibitory action that cadmium grows for root-mustard.
Description
Technical field
The invention belongs to growing vegetables technical field, and in particular to one kind significantly reduces root-mustard Cd accumulation and promotes it to give birth to
Long method.
Background technology
The quality of soil resource has vital influence on human survival.With the fast development of China's industry, agriculture
The content of field heavy metal is greatly increased.In field crop, the major pollutants of agricultural product are heavy metal classes, wherein with cadmium the most
It is prominent.Cadmium has stronger carcinogenic, teratogenesis and mutagenesis, and those can be made to need the physiological function of the enzyme of zinc to be interfered,
Reduce its bioactivity.The too high risk that can cause to suffer from the diseases such as diabetes of cadmium content in human body is raised significantly, but also meeting
Serious zinc-deficiency is made one, causes the appearance of kinds cancer.Plant is very easy to accumulation harmful heavy metal cadmium, because plant is to cadmium
Both can Passive intake, also metabolizable absorption.Cadmium ion is easily concentrated in the protein particulate of plant, and animal and people are eaten up containing cadmium
Plant, cadmium is accumulated in vivo, so as to produce harm to animal and human body.Cadmium is present in plant with the form combined with protein simultaneously
In object, enzymatic activity is set to be interfered.Damage to crops grows, and causes plant biochemistry process disorderly, so as to influence crop
Yield and quality.The agricultural product such as paddy rice, vegetables seriously threaten human body because being grown in by Quality Down in the exceeded soil of cadmium content
Health, influences agricultural sustainable development.The cadmium content in soil environment is reduced, or it is reduced to crops by external means
The pollution of product, it has also become following field of food safety and the focus and difficult point of horticultural field joint research.
Root-mustard (Brassicajuncea) has very abundant nutrition as resident daily consumption vegetables, there is moistening lung, tune
Stomach, treatment constipation, toxin expelling, removing toxic substances, improving eyesight, the healthcare function for improving immunity are managed, all parts of the country there are different advantages to plant
Kind is trained, production area is very huge.
At present, it is one of main means using exogenous hormone, no in the research field of the drop cadmium content of vegetable crop
Cross because Different Crop is different for the sensitiveness of hormon, cause that the screening process of suitable hormone is complex, gained is ground
Conclusion is studied carefully without universality.At present, the research in terms of the drop cadmium of root-mustard is less, meanwhile, it can also delay while cadmium is dropped
Research of the cadmium to the suppression of root-mustard is solved, more rarely has further investigation.
The content of the invention
In view of the shortcomings of the prior art and demand, root-mustard Cd accumulation is significantly reduced it is an object of the invention to provide one kind
And the method for promoting it to grow, it includes step：
When root-mustard seedling grows 3~4 true leaves, leaf is carried out using the epiphysin that concentration is 50~200 μm of olL-1
Face is sprayed, and the amount of spraying is dripped for degree with blade face.
It is preferred that, the concentration of the melatonin is 50~150 μm of olL-1。
It is furthermore preferred that the concentration of the melatonin is 150 μm of olL-1.
Epiphysin (melatonin, Melatonin, MT) is a class weight of generally existing in animal and plant body
The Benzazole compounds wanted.It has important function in terms of Physiological effect, enhancing stress resistance of plant.But, make for different
For thing, exogenous hormone (including epiphysin) is how to improve the resistance of crop and which adverse circumstance to have mitigation to,
Do not draw unified conclusion also at present, the situation that normal appearance effect is completely contradicted, such as《Salicylic acid is sprouted to pea seed under Cd stress
The influence of hair and growth of seedling》Salicylic acid (SA) is reported for pea, with the effect that cadmium is injured is alleviated, still《Cadmium
Toxic effect to rape and apply regulating and controlling effect of the exogenous hormone to cadmium poisoning》But reporting SA can not only alleviate on the contrary
Injury of the cadmium to rape can also be aggravated.
The present invention is also, it was found that other conventional exogenous hormones (such as abscisic acid, gibberellin and EBR) are for cadmium in root-mustard
Content has no reduction effect, and can not also alleviate the suppression that cadmium grows for root-mustard, occurs at higher concentrations or even also again
The situation of suppression.
Beneficial effects of the present invention：
1st, the present invention can significantly reduce the cadmium content in root-mustard；
2nd, the method can significantly improve the Contents of Photosynthetic Pigments of root-mustard, activities of antioxidant enzymes and biomass；
3rd, the present invention can effectively alleviate the inhibitory action that cadmium grows for root-mustard.
Embodiment
The present invention is specifically described below by embodiment, it is necessary to it is pointed out here that be following examples be use
It is further detailed in the present invention, it is impossible to be interpreted as limiting the scope of the invention, the field is skilled in technique
Some nonessential modifications and adaptations that personnel are made according to foregoing invention content, still fall within protection scope of the present invention.
Embodiment 1
1 test material and experiment
1.1 material
Root-mustard seed is purchased from market.Soil is derived from Wenjiang district of Chengdu city farmland, and cadmium is not detected.
1.2 experiment
Soil is air-dried, crushed, the cadmium content added into soil is 10mg/kg pure CdCl2·2.5H2O solution, makes
Cadmium is fully mixed with soil, and the soil (moisture soil) of cadmium pollution is weighed 3.0kg loaded on 15 by soil respectively after standing one month or so
In × 18cm (height × diameter) 30 plastic tubs, the processing to soil is completed.
Root-mustard seed is uniformly planted in the plastic tub for having installed soil, 10 are planted per basin, is kept wet
Profit, is positioned in culturing room, keeps the temperature of culturing room at 24 DEG C or so, moves on in rain shade PVC basins after emerging, educate
Seedling, 4 plants consistent of seedling of growth is retained per basin, and water to keep ground moistening in time.
Treat that root-mustard growth of seedling goes out 3-4 piece true leaves, spraying concentration is 0,50,100,150,200 μm of olL-1Take off it is black
Hormone.Treat that root-mustard regeneration is about two weeks, take the consistent mature leaf of growth site to be used to determine root-mustard Contents of Photosynthetic Pigments
And activities of antioxidant enzymes.Afterwards, whole strain harvest, is cleaned up the soil of root system and aerial part with running water, after by root-mustard
Seedling soaks the measurement of morphological index and cadmium content that plant just can be carried out after 12-24h in deionized water.
2 determine project and method
The measure of 2.1 root-mustard morphological indexs
2.1.1 the measure of root-mustard root length
The root system of root-mustard is cleaned out, unfolds stretching, every plant of most long root is found out, uses tape measuring length.
2.1.2 the measure of plant height
The plant height of every plant of root-mustard is determined with tape measure.
2.1.3 the measure of root tuber diameter and length
Using every plant of root-mustard root tuber diameter of vernier caliper measurement and length.
2.1.4 the measure of each position fresh weight
With the fresh weight of each organ of electronic balance weighing root-mustard seedling.
2.1.5 the measurement of root system volume
The root system of every plant of root-mustard is cut with scissors, is then placed in the graduated cylinder for having filled part water, record is not put
Data before and after entering carry out subtraction, draw root system volume[16]。
2.2 physiological index determining
2.2.1 in root-mustard Contents of Photosynthetic Pigments measure
(1 is mixed using acetone-ethanol:1) it is chlorophyll a, chlorophyll b, chlorophyll total amount that extraction, which determines photosynthetic pigments,
And the content of carotenoid.
2.2.2 the measure of soluble protein
Soluble protein content is determined with coomassie brilliant white G250 methods, with the milli of soluble protein contained by every gram of fresh weight sample
Grams represents (mgg-1)。
2.2.3 the measure of several activities of antioxidant enzymes
Peroxidase (POD), superoxide dismutase (SOD), catalase (CAT) activity according to《Plant physiology
Biochemical test principle and technology》Method be measured.
2.2.4 the measure of cadmium content
It is 4 with volume ratio by sample comminution:Surveyed after 1 nitric acid-perchloric acid nitrification with the type ICP spectrometers of iCAP 6300
It is fixed.
2.3 data statistics and analysis
All data carry out data analysis with Excel, SPSS, calculate and map.
3 results and analysis
Influence of the 3.1 various concentrations epiphysins processing to root-mustard seedling morphology index
As shown in Table 1, with comparing, after epiphysin processing, when epiphysin concentration is in 150 μm of olL-1Within when, greatly
Root length, plant height, root tuber diameter and the root tuber length of head dish seedling all increase, when epiphysin concentration is 100 μm of olL-1When
Improve most, root length and plant height have been respectively increased 47.20% (P ＜ 0.05) and 8.47% (P ＜ 0.05), the raising of root tuber diameter compared with
Greatly, 32.86% (P ＜ 0.05) has been reached, root tuber length also improves 26.68% (P ＜ 0.05).When epiphysin concentration is 200 μ
mol·L-1When, the root length of root-mustard seedling, plant height, root tuber diameter are significantly lower than with root tuber length and compareed.As can be seen here, with
Control is compared, and is less than 150 μm of olL to root-mustard seedling spraying concentration-1Epiphysin can improve, improve the outer of root-mustard seedling
Portion's morphological index, and when concentration reaches 100 μm of olL-1When, most pronounced effects；Excessive concentration is 200 μm of olL-1Epiphysin
Growth to root-mustard seedling plays inhibitory action.
The various concentrations epiphysin of table 1 processing to root-mustard Seedling Height, root length, root tuber diameter, root tuber length influence
Note：Difference reaches the level of signifiance (P ＜ 0.05) between different lowercase letters are respectively handled
Influence of the 3.2 various concentrations epiphysins processing to root-mustard seedling each several part fresh weight and root system volume
As shown in table 2, it is compared with a control, spraying concentration is 50,100,150 μm of olL-1Epiphysin when, root-mustard
Fresh weight and root system volume be improved.When epiphysin concentration is 100 μm of olL-1At most, weight reaches maximum to Shi Tigao
Value, 79.78% (P ＜ 0.05), 23.27% (P has been respectively increased compared with control in root system, root tuber and the aerial part fresh weight of root-mustard
＜ 0.05) and 57.16% (P ＜ 0.05)；Moreover, spray after epiphysin, the root system volume of root-mustard seedling is also carried
Height, epiphysin concentration is 50,100 and 150 μm of olL-1When, 13.31%, 120.42% and has been respectively increased compared with control
34.74%, and significant difference (P ＜ 0.05).When epiphysin concentration is 200 μm of olL-1When, the fresh weight and root system body of root-mustard
Product is significantly lower than control.As can be seen here, using the epiphysin in the range of finite concentration can improve root-mustard seedling fresh weight and
Root system volume, when concentration reaches 100 μm of olL-1When, best results；If epiphysin excessive concentration is 200 μm of olL-1When, take off
Melanocyte starts the growth to root-mustard seedling and plays inhibitory action, declines the fresh weight and root system volume of root-mustard seedling.
Influence of the various concentrations epiphysin of table 2 processing to root-mustard seedling fresh weight and root system volume
Note：Difference reaches the level of signifiance (P ＜ 0.05) between different lowercase letters are respectively handled
Influence of the 3.3 various concentrations epiphysins processing to root-mustard seedling Contents of Photosynthetic Pigments
It can be obtained, be sprayed using epiphysin after processing by table 3, the chlorophyll a of root-mustard seedling, chlorophyll b, chlorophyll total amount
And carotenoid is improved compared with control.With the increase of epiphysin concentration, the degree that its content is improved is first big after small, explanation
Epiphysin can improve the chlorophyll content of root-mustard seedling.From the point of view of difference analysis result, chlorophyll b and contrast difference are not
Significantly (P ＞ 0.05), but its content is being accordingly increased, when concentration is 150 μm of olL-1, 23.14% is improved compared with control.
Epiphysin concentration is 50,100,150 and 200 μm of olL-1When, 12.23% (P has been respectively increased in chlorophyll a<0.05)、
11.66% (P<0.05), 37.38% (P ＜ 0.05) and 9.86% (P>0.05), chlorophyll total amount has been respectively increased 12.91%
(P<0.05), 13.18% (P<0.05), 34.51% (P ＜ 0.05) and 9.71% (P ＜ 0.05)；Carotenoid content is distinguished
Improve 13.57% (P ＞ 0.05), 13.57% (P>0.05), 50.36% (P ＜ 0.05) and 10.80% (P>0.05).From leaf
From the point of view of green plain a/b, while epiphysin concentration increase, ratio first reduces and increased afterwards, when concentration is 150 μm of olL-1When, improve
Value it is maximum, be 11.53%.
Influence of the various concentrations epiphysin of table 3 processing to root-mustard seedling Contents of Photosynthetic Pigments
Note：Difference reaches the level of signifiance (P ＜ 0.05) between different lowercase letters are respectively handled
Influence of the 3.4 various concentrations epiphysins processing to root-mustard seedling antioxidase enzymatic activity and soluble protein
As known from Table 4, with being found after comparing, after being sprayed using epiphysin, POD, SOD, CAT of root-mustard seedling live
Property and soluble protein content show increased situation with the increase of epiphysin concentration.Epiphysin concentration be 50,100,
150 and 200 μm of olL-1When, POD activity respectively compared with control improve 1.28% (P ＞ 0.05), 20.46% (P ＜ 0.05),
26.44% (P ＜ 0.05) and 39.16% (P ＜ 0.05)；SOD, CAT activity increase are relatively large, are 200 μm of ol in concentration
L-1When improve 57.78% (P ＜ 0.05) and 33.33% (P ＜ 0.05) compared with control respectively；Soluble protein content obtains content
It is improved, is 200 μm of olL in epiphysin concentration-1When reach maximum, improve 150% (P ＜ 0.05) compared with control.Thus may be used
See, epiphysin can improve the activities of antioxidant enzymes and soluble protein content of root-mustard seedling, and concentration is 200 μm of olL-1
When best results.
Influence of the various concentrations epiphysin of table 4 processing to root-mustard seedling peroxidase activity and soluble protein
Note：Difference reaches the level of signifiance (P ＜ 0.05) between different lowercase letters are respectively handled
Influence of the 3.5 various concentrations epiphysins processing to root-mustard seedling dry weight cadmium content
As shown in Table 5, compared with the processing that unused epiphysin is sprayed, epiphysin is sprayed after processing, root-mustard seedling
The cadmium content of each organ is decreased, and when epiphysin concentration is stepped up, the cadmium content of root-mustard seedling is being gradually decreased,
Illustrate that epiphysin can reduce the cadmium content of root-mustard seedling.When epiphysin concentration reaches 200 μm of olL-1When, root system, root tuber
Relatively compareed with aerial part cadmium content and reduce 69.23% (P ＜ 0.05), 46.31% (P ＜ 0.05) and 27.81% (P respectively
＜ 0.05).Epiphysin concentration is 50,100,150 and 200 μm of olL-1When, root-mustard edible portion is relatively compareed and reduced respectively
15.10% (P ＜ 0.05), 18.68% (P ＜ 0.05), 18.86% (P ＜ 0.05) and 33.04% (P ＜ 0.05).Thus may be used
See, epiphysin, which is sprayed, can reduce the cadmium content of root-mustard seedling, when concentration is 200 μm of olL-1When,
Effect is best.
Influence of the various concentrations epiphysin of table 5 processing to root-mustard seedling dry weight cadmium content
Note：Difference reaches the level of signifiance (P ＜ 0.05) between different lowercase letters are respectively handled
Comparative example 1
It is 1~200 μm of olL that epiphysin in embodiment 1 is replaced with into concentration-1Abscisic acid, remaining and embodiment 1 one
Cause.
Testing result is：
Suppression of the cadmium for root-mustard can not effectively be alleviated.When concentration is 1~150 μm of olL-1When, plant height, root length, block
Root diameter, root tuber length and for not applying abscisic acid, are almost not different；When concentration is 200 μm of olL-1When, plant height, root
There is 15.2~18.7% decline in length, root tuber diameter, root tuber length.
For the reduction of cadmium content, only up to 4.3%.
Comparative example 2
It is 1~200 μm of olL that epiphysin in embodiment 1 is replaced with into concentration-1Gibberellin, remaining and embodiment 1 one
Cause.
Testing result is：Suppression of the cadmium for root-mustard can not effectively be alleviated.When concentration is 1~100 μm of olL-1When, strain
Height, root length, root tuber diameter, root tuber length and for not applying gibberellin, are almost not different；When concentration is 150~200 μ
mol·L-1When, there is 12.0~25.6% decline in plant height, root length, root tuber diameter, root tuber length.
For the reduction of cadmium content, only up to 2.7%.
Comparative example 3
It is 1~200 μm of olL that epiphysin in embodiment 1 is replaced with into concentration-1EBR, remaining is consistent with embodiment 1.
Testing result is：Suppression of the cadmium for root-mustard can not effectively be alleviated.When concentration is 1~200 μm of olL-1When, strain
Height, root length, root tuber diameter, root tuber length and for not applying EBR, are almost not different.
For the reduction of cadmium content, only up to 3.1%.
Claims (3)
1. a kind of method for significantly reducing root-mustard Cd accumulation and promoting it to grow, it is characterised in that methods described includes step：
It is 50~200 μm of olL using concentration when root-mustard seedling grows 3~4 true leaves-1Epiphysin carry out blade face spray
Apply, the amount of spraying is dripped for degree with blade face.
2. according to the method described in claim 1, it is characterised in that the concentration of the melatonin is 50~150 μm of olL-1。
3. according to the method described in claim 1, it is characterised in that the concentration of the melatonin is 150 μm of olL-1。
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Cited By (6)
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| CN107041181A (en) * | 2017-04-14 | 2017-08-15 | 四川农业大学 | It is a kind of to promote pea seedling growth, the method for reduction Cd accumulation |
| CN108207960A (en) * | 2018-01-04 | 2018-06-29 | 山东农业大学 | Application of the external source epiphysin to Strawberry seedlings Cd stress relieving effect |
| CN108660104A (en) * | 2018-04-27 | 2018-10-16 | 西北大学 | Application of the external source epiphysin in improving Chlamydomonas reinhardtii saline-alkaline tolerance |
| CN110839618A (en) * | 2019-11-18 | 2020-02-28 | 浙江中环农业开发有限公司 | Nano protective agent for improving stress resistance of crops and preparation method thereof |
| CN112335662A (en) * | 2020-10-22 | 2021-02-09 | 南京农业大学 | Separating agent for heavy metal cadmium in pakchoi and using method of separating agent |
| CN115152542A (en) * | 2022-05-24 | 2022-10-11 | 四川农业大学 | Application method of brassinolide leaf surface blocking and controlling agent in Chinese cabbage planting |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107041181A (en) * | 2017-04-14 | 2017-08-15 | 四川农业大学 | It is a kind of to promote pea seedling growth, the method for reduction Cd accumulation |
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| CN108660104A (en) * | 2018-04-27 | 2018-10-16 | 西北大学 | Application of the external source epiphysin in improving Chlamydomonas reinhardtii saline-alkaline tolerance |
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| CN112335662A (en) * | 2020-10-22 | 2021-02-09 | 南京农业大学 | Separating agent for heavy metal cadmium in pakchoi and using method of separating agent |
| CN115152542A (en) * | 2022-05-24 | 2022-10-11 | 四川农业大学 | Application method of brassinolide leaf surface blocking and controlling agent in Chinese cabbage planting |
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