CN115152542A - Application method of brassinolide leaf surface blocking and controlling agent in Chinese cabbage planting - Google Patents
Application method of brassinolide leaf surface blocking and controlling agent in Chinese cabbage planting Download PDFInfo
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- CN115152542A CN115152542A CN202210576096.0A CN202210576096A CN115152542A CN 115152542 A CN115152542 A CN 115152542A CN 202210576096 A CN202210576096 A CN 202210576096A CN 115152542 A CN115152542 A CN 115152542A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/15—Leaf crops, e.g. lettuce or spinach
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
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Abstract
The invention discloses a method for using a brassinolide foliage resistance and control agent in Chinese cabbage planting, which comprises the following steps: step one, adding brassinolide into ultrapure water, stirring at a certain temperature until the brassinolide is completely dissolved to obtain a brassinolide solution with the concentration of 5mg/L; step two, taking the brassinolide solution obtained in the step one, transferring the brassinolide solution into a beaker, adding ultrapure water to obtain brassinolide solutions with the concentrations of 0.1mg/L, 0.3mg/L and 0.5mg/L respectively, and collecting and storing the brassinolide solutions to obtain the leaf surface resistance and control agent; step three, spraying the first foliage resistance control agent when the Chinese cabbages grow to the three-leaf one-heart leaves, wherein the spraying concentration is 0.5mg/L; and step four, spraying is also carried out on days 3,6 and 9 after the first spraying, and the spraying concentrations are 0.3mg/L,0.1mg/L and 0.1mg/L respectively for four times. After the foliage resistance and control agent is sprayed on the leaves of the pakchoi, the plant biomass can be effectively increased, and the transfer of cadmium to the edible parts of the pakchoi is prevented and controlled.
Description
Technical Field
The invention relates to the technical field of heavy metal pollution farmland treatment, in particular to a method for using a brassinolide foliage resistance and control agent in Chinese cabbage planting.
Background
Arable soil is a natural resource on which humans rely for survival. Cadmium in the soil has the characteristics of long decomposition period, high mobility, difficult degradation and the like, can generate toxic action on plants, can be concentrated and amplified along with food chains, and finally generates harm to human health. Therefore, the method effectively prevents and controls the accumulation of cadmium in agricultural products and is an important technical measure for ensuring the food safety.
The cadmium enrichment effect of leaf vegetables is obviously higher than that of other vegetables. The pakchoi is one of leaf vegetables with high enrichment on cadmium. Even in the soil with slight cadmium pollution, the pakchoi still has higher cadmium enrichment amount. Therefore, the safe production of pakchoi is not negligible.
Among numerous heavy metal resistance control technologies, passivation remediation has the characteristics of good resistance control effect, simplicity in operation and the like, and is suitable for remediation of large-area polluted farmlands, but long-term application of the passivator can destroy the granular structure of soil, cause soil hardening, and further influence the growth of crops.
The leaf surface resistance control technology is considered as an effective means for reducing the cadmium accumulation of crops, has the characteristics of high efficiency, rapidness and safety, and has the potential of large-area popularization and application. Many non-metal, metal and organic materials used as the foliage resistance and control agent show good cadmium reduction effect. However, some of these inhibitors are not effective and even present an environmental risk. For example, sodium selenite is used as a foliage spray of grapes, the content of organic selenium in plant autolysate is low, the biological activity is low, and the toxic and side effects are great. The brassinolide is used as an environment-degradable and safe plant growth regulator, and has the effects of increasing plant biomass and promoting plant growth. Chinese patents with publication numbers CN108812112A respectively disclose a method for improving cadmium enrichment of grafted shepherd's purse progeny by combined application of hymexazol and brassinolide, but the improvement of cadmium enrichment in plants applied by the above patents can be used for increasing the absorption of heavy metals in soil by plants. It is not clear whether brassinolide has the effect of reducing the cadmium content of pakchoi. In summary, a leaf surface resistance control agent for reducing the cadmium content of the pakchoi by using brassinolide and a using method thereof need to be designed.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a method for using a brassinolide foliage resistance and control agent in planting of pakchoi.
In order to achieve the purpose, the invention provides the following technical scheme: a method for using a brassinolide foliage resistance and control agent in Chinese cabbage planting comprises the following steps:
step one, adding brassinolide into ultrapure water, stirring at a certain temperature until the brassinolide is completely dissolved to obtain a brassinolide solution with the concentration of 5mg/L;
step two, taking the brassinolide solution obtained in the step one, transferring the brassinolide solution into a beaker, adding ultrapure water to obtain brassinolide solutions with the concentrations of 0.1mg/L, 0.3mg/L and 0.5mg/L respectively, and collecting and storing the brassinolide solutions to obtain the leaf surface resistance and control agent;
step three, spraying a first foliage resistance and control agent after the Chinese cabbages grow to three leaves and one heart;
step four, spraying is also carried out on the 3 rd, 6 th and 9 th days after the first spraying, and the spraying is carried out for four times.
Further, in the first step, the certain temperature is specifically 40-50 ℃.
Further, in the third step, the first spraying of the foliage resistance and control agent refers to spraying the foliage resistance and control agent with the concentration of 0.5 mg/L.
Further, in the fourth step, the spraying of the foliar resistance control agent on the 3 rd, 6 th and 9 th days after the first spraying refers to the spraying concentrations of 0.3mg/L,0.1mg/L and 0.1mg/L respectively.
Further, in the third step or the fourth step, the spraying is carried out until the foliage drops.
Further, in the spraying of the third step or the fourth step, the spraying time is in the evening, and the spraying parts are the surfaces and the back surfaces of the leaves.
Furthermore, after the leaf surface control agent adopting brassinolide is sprayed on the pakchoi, the transfer of cadmium from soil to the overground part can be well inhibited, and the transfer coefficient is as low as 0.76.
Furthermore, after the brassinolide foliage resistance and control agent is sprayed on the pakchoi, the biomass of the stem leaf part and the root part of the pakchoi is increased, the dry weight of the stem leaf part of the pakchoi can reach 25.96g respectively, and the dry weight of the root part of the pakchoi can reach 1.01g respectively.
The beneficial effects of the invention are: after the foliage resistance and control agent is sprayed on the leaves of the pakchoi, the plant biomass can be effectively increased, and the transfer of cadmium to the edible parts of the pakchoi can be prevented and controlled. 0.5mg L of first spraying in one heart period of three leaves -1 Then 0.3mg/L,0.1mg/L and 0.1mg/L leaf surface resistance control agents are respectively sprayed on the 3 rd, 6 th and 9 th days, so that the transfer of cadmium from soil to the overground part can be well inhibited, and the transfer coefficient is also minimum under secondary concentration and is 0.76; the dry weight of the stem and leaf parts and the root parts of the pakchoi respectively reach the maximum value of 25.96g and 1.01g.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
The invention provides a preparation method of a leaf surface resistance control agent, the main raw material of the preparation is brassinolide, and the preparation method comprises the following steps:
(1) The purchased brassinolide is 5mg, and the solubility of the brassinolide at normal temperature is about 5mg/kg, so that the brassinolide is prepared by firstly adding 1L of UP water into a beaker which is cleaned by distilled water and rinsed by ultrapure water, then pouring 5mg of brassinolide into water, and stirring at the temperature of 40-50 ℃ until the solid is completely dissolved to prepare 5mg/L of brassinolide solution.
(2) And (2) taking 100mL (containing 0.5mg of brassinolide) of the brassinolide solution with the concentration of 5mg/L obtained in the step (1), transferring the brassinolide solution into a beaker which is cleaned by distilled water and is rinsed by UP water, adding water to 1L to prepare the leaf surface inhibition and control agent with the concentration of 0.5mg/L, and bottling, capping and storing.
(3) And (2) taking 60mL (containing 0.3mg of brassinolide) of the brassinolide solution with the concentration of 5mg/L obtained in the step (1), transferring the brassinolide solution into a beaker which is cleaned by distilled water and is rinsed by UP water, adding water to 1L to prepare a leaf surface inhibition and control agent with the concentration of 0.3mg/L, and bottling, capping and storing.
(4) And (2) taking 20mL (containing 0.1mg of brassinolide) of the brassinolide solution with the concentration of 5mg/L obtained in the step (1), transferring the brassinolide solution into a beaker which is cleaned by distilled water and is rinsed by UP water, adding water to 1L to prepare a leaf surface inhibition and control agent with the concentration of 0.1mg/L, and bottling, capping and storing.
The invention provides a use method for reducing the cadmium content of pakchoi in medium and light cadmium polluted soil by using the preparation, which comprises the following steps:
(1) Spraying for the first time when the Chinese cabbage grows to three leaves and one heart;
(2) Spraying is carried out 3,6 and 9 days after the first spraying, and four times of spraying is carried out;
(3) The spraying time is clear and in the evening, and the parts are the surfaces and the back surfaces of the leaves of the pakchoi. The liquid is sprayed to each leaf for dripping each time.
According to the preparation method, the prepared concentrations are respectively 0.1, 0.3 and 0.5mg L -1 The foliage retardant solution is sprayed on the foliage of the pakchoi in the period from growing to three-leaf one-heart, and clear water is sprayed to serve as a control CK. Continuously spraying the growth of the pakchoi in 3 rd, 6 th and 9 th days after the first spraying, wherein the spraying amount is the same for four times. The spraying scheme is divided into three schemes A, B and C, and the specific spraying methodThe methods and concentrations are shown in Table 1. The spraying time is evening, the parts are the surface and the back of the leaf, and the spraying is carried out until the liquid drops on the leaf surface.
Selecting a fine morning 9:00 to 11: and measuring the light and parameters of the pakchoi about 00. Harvesting pakchoi after the tenth day, and measuring fresh dry weight and cadmium content.
Table 1 three spray application protocols and spray application methods
Results of the experiment
After the foliage resistance and control agent with different concentrations is sprayed, the concentration of the cadmium content in the stem leaves of the pakchoi promotes the cadmium accumulation, and the cadmium accumulation is inhibited by high concentration; the cadmium content of the roots of the pakchoi increases along with the increase of the spraying concentration of the foliage resistance and control agent. As shown in Table 2, when the spraying scheme of the leaf surface control agent is A, the cadmium content of the stem and leaf parts of the pakchoi is increased by 57.64 percent compared with the CK group, and the cadmium content of the root parts of the pakchoi is increased by 102.40 percent compared with the blank group; when the foliage resistance and control agent is sprayed to the scheme B, the cadmium content of the stem leaves of the pakchoi is increased by 90.00 percent compared with that of a CK group, and the cadmium content of the roots of the pakchoi is increased by 27.90 percent compared with that of a blank group; when the foliage resistance control agent is sprayed on the scheme C, the cadmium content of the stem leaves of the pakchoi is reduced by 20.52 percent compared with that of the CK group, and the cadmium content of the roots of the pakchoi is increased by 154.16 percent compared with that of the blank group. The method shows that the foliage control agent sprayed according to the scheme C can better inhibit the cadmium from being transported from the soil to the overground part, and the transport coefficient of the foliage control agent is minimum under the scheme and is 0.76.
TABLE 2 influence of EBL spraying at different concentrations on the cadmium content of pakchoi
Note: the lower case letters in the same column indicate significant differences between treatments (P < 0.05).
Compared with CK, the three schemes of spraying the leaf surface control agent all increase the biomass of the stem leaf part and the root part of the pakchoi, and are shown in the table 3. When the spraying scheme is C, the dry weights of the stem leaves and the roots of the pakchoi respectively reach the maximum values of 25.96g and 1.01g.
TABLE 3 influence of EBL spraying at different concentrations on the dry weight of pakchoi
After the foliage resistance and control agent is sprayed on the leaves of the pakchoi, the plant biomass can be effectively increased, and the transfer of cadmium to the edible parts of the pakchoi is prevented and controlled.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some features of the invention can be made without departing from the spirit and scope of the invention.
Claims (8)
1. A use method of a brassinolide foliage resistance and control agent in Chinese cabbage planting is characterized by comprising the following steps:
step one, adding brassinolide into ultrapure water, stirring at a certain temperature until the brassinolide is completely dissolved to obtain a brassinolide solution with the concentration of 5mg/L;
step two, taking the brassinolide solution obtained in the step one, transferring the brassinolide solution into a beaker, adding ultrapure water to obtain brassinolide solutions with the concentrations of 0.1mg/L, 0.3mg/L and 0.5mg/L respectively, and collecting and storing the brassinolide solutions to obtain the leaf surface resistance and control agent;
step three, spraying a first foliage resistance and control agent after the Chinese cabbages grow to three leaves and one heart;
and step four, spraying the mixture on 3,6 and 9 days after the first spraying, wherein the spraying is carried out for four times.
2. The use method of the brassinolide foliage resistance and control agent in the planting of the pakchoi as claimed in claim 1, characterized in that: in the first step, the certain temperature is 40-50 ℃.
3. The use method of the brassinolide foliage resistance and control agent in the planting of the pakchoi as claimed in claim 1, characterized in that: in the third step, the first spraying of the foliar retarding and controlling agent refers to spraying of the foliar retarding and controlling agent with the concentration of 0.5 mg/L.
4. The use method of the brassinolide foliage resistance and control agent in the planting of the pakchoi as claimed in claim 1, characterized in that: in the fourth step, the spraying of the foliar resistance-controlling agent on the 3,6 and 9 th days after the first spraying refers to the spraying concentrations of 0.3mg/L,0.1mg/L and 0.1mg/L respectively.
5. The use method of the brassinolide foliage resistance and control agent in the planting of the pakchoi as claimed in claim 1, characterized in that: in the third step or the fourth step, the spraying is carried out until the spraying is carried out on the leaf surface drops.
6. The use method of the brassinolide foliage resistance and control agent in pakchoi planting according to claim 1 or 5, characterized by comprising the following steps: in the spraying in the third step or the fourth step, the spraying time is in the evening, and the spraying parts are the surfaces and the back surfaces of the leaves.
7. The use method of the brassinolide foliar-controlling agent in the planting of pakchoi as claimed in any one of claims 1 to 6, wherein: after the leaf surface control agent adopting the brassinolide is sprayed on the pakchoi, the transfer of cadmium from soil to the overground part can be well inhibited, and the transfer coefficient is as low as 0.76.
8. The use method of the brassinolide foliage resistance and control agent in the planting of the pakchoi as claimed in claim 7, characterized in that: after the brassinolide foliage resistance and control agent is sprayed on the pakchoi, the biomass of the stem leaf part and the root part of the pakchoi is increased, the dry weight of the stem leaf part of the pakchoi can reach 25.96g respectively, and the dry weight of the root part of the pakchoi can reach 1.01g respectively.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210576096.0A CN115152542A (en) | 2022-05-24 | 2022-05-24 | Application method of brassinolide leaf surface blocking and controlling agent in Chinese cabbage planting |
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| CN202210576096.0A CN115152542A (en) | 2022-05-24 | 2022-05-24 | Application method of brassinolide leaf surface blocking and controlling agent in Chinese cabbage planting |
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Cited By (1)
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|---|---|---|---|---|
| CN116548260A (en) * | 2023-05-09 | 2023-08-08 | 安徽农业大学 | Method for promoting conversion of flat leaf surfaces of cabbages into wrinkled leaf surfaces |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116548260A (en) * | 2023-05-09 | 2023-08-08 | 安徽农业大学 | Method for promoting conversion of flat leaf surfaces of cabbages into wrinkled leaf surfaces |
| CN116548260B (en) * | 2023-05-09 | 2024-02-09 | 安徽农业大学 | Method for promoting conversion of flat leaf surfaces of cabbages into wrinkled leaf surfaces |
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