CN103980030A - Preparation for reducing accumulation of cadmium in crops and application method thereof - Google Patents
Preparation for reducing accumulation of cadmium in crops and application method thereof Download PDFInfo
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- CN103980030A CN103980030A CN201410056774.6A CN201410056774A CN103980030A CN 103980030 A CN103980030 A CN 103980030A CN 201410056774 A CN201410056774 A CN 201410056774A CN 103980030 A CN103980030 A CN 103980030A
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Abstract
The invention belongs to the fields of soil heavy-metal pollution treatment in environment protection and agricultural product safety, and relates to a preparation for reducing accumulation of cadmium in crops and an application method thereof. The preparation for reducing accumulation of cadmium in crops comprises the compositions with the following concentration: 50-80 g/L of reduced glutathione, 15-30 mg/L of sodium selenite and 2.0-3.0*10<3> mg/L of zinc sulfate. The crops mean paddy rice or barley. The preparation has the advantages of preparing simpliness, usage convenience, no toxic and side effects, low cost and the like. The preparation is used by spraying on leaf surfaces, the operation is similar to operation of spraying pesticides, the application method is simple and easy to operate, and the preparation is also capable of improving the content of zinc, selenium and other nutritional elements in the crops. The preparation is applicable to paddy rice, barley and other crops for producing low-cadmium products, and is suitable for popularization application in moderate/mild cadmium-pollution regions.
Description
Technical field
The invention belongs to improvement and the agricultural product security field of heavy metal pollution of soil in environment protection, relate to a kind of preparation and using method thereof that cadmium accumulates in farm crop that reduce.
Background technology
Cadmium (Cadmium, Cd) is one of the strongest and farmland the most serious contaminated heavy metal of known toxicity.Meanwhile, the transport property of Cd is stronger, is easily absorbed by plants and is transferred to overground part, exceedes certain limit and not only crop growth is damaged, and causes yield and quality to decline, and more likely threatens human health by food chain.Therefore, reduce Cd accumulation and can not only improve the quality of farm crop, the protection eubiosis, also can reduce Cd and bring the mankind's health risk.It is reported, rice cadmium pollution is the subject matter of Japanese heavy metal contamination; The determination and analysis of Japan, the edible Cereal seed in Korea S and China different areas shows, many sample cadmium contents obviously exceed the top limit (0.1 μ g/g) (Watanabe etc., 1996) that FAO/WHO recommends.According to investigations, China is subject to the arable land of cadmium pollution to exceed 1.33 ten thousand hm2, relates to 11 and economizes 25 areas.Nineteen fifty-five is to the beginning of the seventies in last century, in Japan folic hill city, basin, magic river once caused the second nuisance disease-" itai-itai disease " because rice cadmium content exceeds standard, successively cause people's death more than 80, even if government and the common people also turn pale at the mention of the tiger even to this day, government has worked out strict standard-required to the cadmium content of various food for this reason.In a word, cadmium pollution the Sustainable development to agriculture production and the mankind's life quality produced huge threat (Davis, 1984).Control and the cadmium pollution of reduction farm crop are urgently to be resolved hurrily.
Reductive glutathione (GSH) is a kind of a kind of small molecules free mercaptan tripeptides being extensively present in plant and animal, the many vital processes such as removing and amino acid whose cross-film transport that maintain albumen thiol levels, mercaptan and disulfide exchange, hydroperoxide and free radical are played an important role to (Mendoza-Co ' zat etc., 2005).The distinctive redox of GSH and nucleophilic characteristic, make it participate in resisting multiple toxic reaction (Ferguson and the Booth1998 including exogenous material, oxyradical, salt damage, acid evil and heavy metal; Wu etc. 2003).Chen etc. (2010) find can significantly alleviate to adding GSH in Cd solution the accumulation that barley growth that Cd causes suppresses and reduce Cd and cause superoxide anion, hydrogen peroxide and mda.GSH can adjusting and controlling rice in activities of antioxidant enzymes and then resist Cd and Cr poisons (Cai etc., 2011; Zeng etc., 2012).Selenium (Se) is essential trace element in human and animal's body, and its biochemical function is widely studied (Hartikainen, 2005).Although Se is nonessential element for plant, there are some researches show that Se can improve plant growth rate, suppresses film lipid peroxidation and increase resistance of oxidation (Djanaguiraman etc., 2005).Zinc (Zn) is the necessary trace element of plant-growth, has antagonistic action (Aravind etc., 2009) with Cd simultaneously.Wu etc. (2002) find that Zn can significantly reduce the accumulation of Cd in barley.In tomato, also find similar result (Mohammad etc., 2009).Zn can also be alleviated the growth-inhibiting (Chen etc., 2007) that mercury causes.Silicon (Si) is one of element that plant materials intensive amount is higher, and the 0.1%-10% that accounts for plant dry weight is not etc.Paddy rice is typically to like silicon crop, and the absorption to silicon and accumulation ability are very strong; Add silicon and significantly reduce Cd content in rice root, cauline leaf and brown rice (Zeng Fanrong, 2010).
Although above research can be alleviated cadmium poisoning, are all the modes that adopt relief factor and Cd to cultivate altogether, in practical study and production, also there is no practicable method; Consider production application, this kind of mode faces the series of problems such as amount of application is relatively bigger than normal, high expensive.
Summary of the invention
The invention provides a kind of preparation that cadmium accumulates in farm crop that reduces, this preparation carries out in the mode of foliage-spray, is easy to preparation, simultaneously easy and simple to handle, with low cost, is highly suitable for agriculture Production of Large Fields.
The present invention also provides the using method of the preparation that above-mentioned reduction cadmium accumulates in farm crop.
The technical solution adopted for the present invention to solve the technical problems is:
Reduce the preparation that cadmium accumulates in farm crop, the component that said preparation comprises following concentration: reductive glutathione
50-80mg/L, Sodium Selenite 15-30mg/L, zinc sulfate 2.0-3.0 × 10
3mg/L.Described farm crop are paddy rice or barley.
As preferably, said preparation also comprises the B solution that need deposit separately, and B solution is 2.5-3.5 × 10
3the sodium silicate solution of mg/L.
As preferably, said preparation is made up of the component of following concentration: A solution: reductive glutathione 50mg/L, Sodium Selenite 15mg/L, zinc sulfate 2.0 × 10
3mg/L, B solution: water glass 2.84 × 10
3mg/L.This formula is mainly used in the accumulation that reduces cadmium in paddy rice.Within 2 years, field experiment result shows, in different rice varieties seeds, Cd content all significantly declines.
A using method for described preparation, specifically: with atomizer, said preparation is evenly sprayed in farm crop blade surface wet to evenly hanging, the time of spraying be controlled at before 9 of mornings or at dusk 4 carry out later.The present invention adopts the mode of foliage-spray, simple to operate, with low cost, and amount of application is relatively little.
The invention has the beneficial effects as follows: preparation of the present invention have preparation simple, easy to use, have no side effect and low cost and other advantages.Mode with foliage-spray when use is carried out, and sprays insecticide similarly, simple to operation, and said preparation can improve the nutritive element contents such as crop zinc, selenium simultaneously.Compared with control group, rice grain Cd average content significantly declined 28.5% and 32.1% respectively in 2011 and 2012.Barley sprays GSH+Se+Zn and significantly reduces 39.3% and 24.6% respectively.The present invention is applicable to the farm crop such as paddy rice and barley and produces low cadmium product, is adapted at mild or moderate Cd IA and applies.
Embodiment
Below by specific embodiment, technical scheme of the present invention is described in further detail.Should be appreciated that enforcement of the present invention is not limited to the following examples, any pro forma accommodation that the present invention is made and/or change all will fall into protection domain of the present invention.
In the present invention, if not refer in particular to, all part, per-cents are weight unit, and equipment and the raw material etc. adopting all can be buied from market or this area is conventional.Method in following embodiment, if no special instructions, is the ordinary method of this area.
Embodiment 1, this preparation is for reducing the cadmium in paddy rice
The component and the concentration that reduce Cd accumulation preparation are as follows:
A solution: reductive glutathione 50mg/L, Sodium Selenite 15mg/L, zinc sulfate 2.0 × 10
3mg/L,
B solution: water glass 2.84 × 10
3mg/L.
Compound method: between seedling stage/tillering phase and heading stage/full heading time foliage-spray A solution first respectively, spray again A solution after 10 days, spray B solution heading stage.In addition, control group and treatment group are respectively taking ammonium nitrate and ammonium sulfate as nitrogenous source.Ripening stage gets rice grain and measures heavy metal content.The results are shown in Table 1.
Table 1 is processed and control group rice grain heavy metal content (mg kg
-1) and output (kg ha
-1)
*, process the percentage reducing compared with the control.2011 and the rice varieties of participating in the experiment in 2012 are respectively 7 and 4.A, b represents 0.05 level difference significance.
Table 2 is processed and control group rice kernel amino acid content (g100g
-1)
Asp, Thr, Glu, Ser, Gly, Ala, Cys, Val, Met, Ile, Leu, Tyr, Phe, Lys, His, Arg, Pro and Total represent respectively aspartic acid, Threonine, L-glutamic acid, Serine, glycine, L-Ala, halfcystine, α-amino-isovaleric acid, methionine(Met), Isoleucine, leucine, tyrosine, phenylalanine, Methionin, Histidine, arginine, proline(Pro) and total amino acid.A, b represents 0.05 level difference significance.
Table 3 is processed and control group rice grain long-width ratio, glue denseness, amylose starch and protein content
A, b represents 0.05 level difference significance.
Embodiment 2, this preparation is for reducing the cadmium in barley
The component and the concentration that reduce Cd accumulation preparation are as follows: reductive glutathione 50mg/L, Sodium Selenite 15mg/L, zinc sulfate 2.0 × 10
3mg/L.
Compound method: be uniformly mixed into after solution by above-mentioned each component, till two leaf one heart stage foliage-sprays are extremely moistening, spray in every 5 days once, is sprayed 2 times altogether; Spray for the first time sampling in latter 10 days, repeat every basin kind 12 strains for 8 times.Different treatment Cd content is in table 4.
Table 4 foliage-spray GSH+Se+Zn coerces the impact of lower barley Cd content and biomass on Cd
*, the percentage of Cd+GSH+Se+Zn treatment group reduction compared with Cd treatment group.A, b and c represent 0.05 level difference significance.
Compared with control group, rice grain Cd average content significantly declined 28.5% and 32.1% respectively in 2011 and 2012.Barley sprays GSH+Se+Zn makes overground part and underground part Cd content significantly reduce 39.3% and 24.6% respectively.The present invention is applicable to paddy rice and barley, is adapted at mild or moderate Cd IA and applies.At present, the report of foliage-spray reduction paddy rice Cd accumulation is fewer, and is all to carry out in greenhouse.And compared with the control, after foliage-spray, in rice yield and seed, aminoacids content, amylose content, protein content and glue denseness significantly rise or remain unchanged.Therefore, said preparation, for actual Rice Production, has more using value.And on barley, the document that foliage-spray reduces Cd accumulation does not almost have.
Embodiment 3, this preparation is for reducing the cadmium in paddy rice
The component and the concentration that reduce Cd accumulation preparation are as follows: A solution: reductive glutathione 65mg/L, Sodium Selenite 22mg/L, zinc sulfate 2.5 × 10
3mg/L, B solution: water glass 2.5 × 10
3mg/L.Using method is with embodiment 1.
Embodiment 4, this preparation is for reducing the cadmium in paddy rice
The component and the concentration that reduce Cd accumulation preparation are as follows: A solution: reductive glutathione 85mg/L, Sodium Selenite 28mg/L, zinc sulfate 3.0 × 10
3mg/L, B solution: water glass 3.5 × 10
3mg/L.Using method is with embodiment 1.
Embodiment 5, this preparation is for reducing the cadmium in barley
The component and the concentration that reduce Cd accumulation preparation are as follows: reductive glutathione 80mg/L, Sodium Selenite 30mg/L, zinc sulfate 3.0 × 10
3mg/L.
Embodiment 6, this preparation is for reducing the cadmium in barley
The component and the concentration that reduce Cd accumulation preparation are as follows: reductive glutathione 60mg/L, Sodium Selenite 25mg/L, zinc sulfate 2.5 × 10
3mg/L.
Above-described embodiment is preferably scheme of one of the present invention, not the present invention is done to any pro forma restriction, also has other variant and remodeling under the prerequisite that does not exceed the technical scheme that claim records.
Claims (5)
1. reduce the preparation that cadmium accumulates in farm crop, it is characterized in that the component that said preparation comprises following concentration:
Reductive glutathione 50-80 mg/ L,
Sodium Selenite 15-30mg/ L,
Zinc sulfate 2.0-3.0 × 10
3mg/ L.
2. the preparation that reduction cadmium according to claim 1 accumulates in farm crop, is characterized in that: described farm crop are paddy rice or barley.
3. the preparation that reduction cadmium according to claim 1 and 2 accumulates in farm crop, is characterized in that: said preparation also comprises the B solution that need deposit separately, and B solution is 2.5-3.5 × 10
3the sodium silicate solution of mg/ L.
4. the preparation that reduction cadmium according to claim 1 accumulates in farm crop, is characterized in that said preparation is made up of the component of following concentration:
A solution: reductive glutathione 50mg/ L, Sodium Selenite 15mg/ L, zinc sulfate 2.0 × 10
3mg/ L,
B solution: water glass 2.84 × 10
3mg/ L.
5. a using method for preparation claimed in claim 1, is characterized in that: with atomizer, said preparation is evenly sprayed in farm crop blade surface wet to evenly hanging, the time of spraying be controlled at before 9 of mornings or at dusk 4 carry out later.
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Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104350988A (en) * | 2014-09-05 | 2015-02-18 | 常州大学 | Rice field selenium application method for reducing Cd concentration in rice |
| CN104402606A (en) * | 2014-11-20 | 2015-03-11 | 长沙健硒保生物科技有限公司 | Cadmium inhibiting rice nutritional agent as well as preparation method and application method thereof |
| CN104478555A (en) * | 2014-11-17 | 2015-04-01 | 浙江大学 | Method for guaranteeing safe output of rice planted in moderate and mild cadmium contaminated soil and preparation used in method |
| CN104488965A (en) * | 2014-11-17 | 2015-04-08 | 浙江大学 | Preparation capable of reducing mercury content in paddy rice grains and usage method thereof |
| CN105010391A (en) * | 2015-06-30 | 2015-11-04 | 湖南农业大学 | Rice cadmium reduction agent |
| CN105613578A (en) * | 2016-02-04 | 2016-06-01 | 太原科技大学 | Composition, preparation and method for lowering cadmium accumulation in cucumber |
| CN106259329A (en) * | 2016-07-20 | 2017-01-04 | 湖南农业大学 | A kind of Oryza sativa L. resistance cadmium agent |
| CN107306952A (en) * | 2017-06-02 | 2017-11-03 | 杭州师范大学 | Glutamine suppresses the method that pakchoi Pb absorbs and improves anti-Pb pollution capacities |
| CN107986863A (en) * | 2017-12-07 | 2018-05-04 | 湖南省农业环境生态研究所 | A kind of water-soluble fertilizer of blade face resistance control rice Cd uptake and its preparation method and application |
| CN109824438A (en) * | 2019-03-26 | 2019-05-31 | 江西省农业科学院土壤肥料与资源环境研究所 | Rice cadmium is enriched with inhibitor and its application technology |
| CN110066202A (en) * | 2019-05-27 | 2019-07-30 | 农业农村部环境保护科研监测所 | Reduce the foliage spraying agent and method of Cd concentration of brown rice |
| CN110149869A (en) * | 2019-05-28 | 2019-08-23 | 农业农村部环境保护科研监测所 | Inhibit the preparation and method of cadmium enrichment in rice |
| CN110934152A (en) * | 2019-12-11 | 2020-03-31 | 浙江大学 | Seed soaking agent and seed soaking method for reducing cadmium and arsenic content in rice grains |
| CN111316805A (en) * | 2020-04-14 | 2020-06-23 | 株洲香之优农业科技发展有限责任公司 | Production method of selenium-rich, zinc-rich, cadmium-reducing and yield-increasing high-quality rice and selenium-rich, zinc-rich and high-quality nutritional rice |
| CN111887250A (en) * | 2020-07-31 | 2020-11-06 | 农业农村部环境保护科研监测所 | Rice leaf surface conditioner and preparation and application methods thereof |
| CN112961003A (en) * | 2021-02-26 | 2021-06-15 | 四川嘉道博文生态科技有限公司 | Base material for reducing cadmium in ligusticum wallichii planting, fertilizer and application thereof |
| CN113951254A (en) * | 2021-11-17 | 2022-01-21 | 浙江大学 | Nano-type leaf surface regulating agent capable of improving cadmium tolerance of rice and preparation method thereof |
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| CN104350988A (en) * | 2014-09-05 | 2015-02-18 | 常州大学 | Rice field selenium application method for reducing Cd concentration in rice |
| CN104478555A (en) * | 2014-11-17 | 2015-04-01 | 浙江大学 | Method for guaranteeing safe output of rice planted in moderate and mild cadmium contaminated soil and preparation used in method |
| CN104488965A (en) * | 2014-11-17 | 2015-04-08 | 浙江大学 | Preparation capable of reducing mercury content in paddy rice grains and usage method thereof |
| CN104478555B (en) * | 2014-11-17 | 2017-03-08 | 浙江大学 | Ensure the safe rice growth output method of cadmium mild or moderate contaminated soil plantation and preparation used |
| CN104488965B (en) * | 2014-11-17 | 2017-03-08 | 浙江大学 | Reduce the preparation of mercury content and its preparation method and usage in rice grain |
| CN104402606A (en) * | 2014-11-20 | 2015-03-11 | 长沙健硒保生物科技有限公司 | Cadmium inhibiting rice nutritional agent as well as preparation method and application method thereof |
| CN105010391B (en) * | 2015-06-30 | 2018-01-19 | 佛山金葵子植物营养有限公司 | Cadmium agent drops in rice |
| CN105010391A (en) * | 2015-06-30 | 2015-11-04 | 湖南农业大学 | Rice cadmium reduction agent |
| CN105613578A (en) * | 2016-02-04 | 2016-06-01 | 太原科技大学 | Composition, preparation and method for lowering cadmium accumulation in cucumber |
| CN106259329A (en) * | 2016-07-20 | 2017-01-04 | 湖南农业大学 | A kind of Oryza sativa L. resistance cadmium agent |
| CN107306952B (en) * | 2017-06-02 | 2021-04-13 | 杭州师范大学 | Method for inhibiting Pb absorption of pakchoi and improving Pb pollution resistance by glutamine |
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| CN107986863A (en) * | 2017-12-07 | 2018-05-04 | 湖南省农业环境生态研究所 | A kind of water-soluble fertilizer of blade face resistance control rice Cd uptake and its preparation method and application |
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| CN110934152A (en) * | 2019-12-11 | 2020-03-31 | 浙江大学 | Seed soaking agent and seed soaking method for reducing cadmium and arsenic content in rice grains |
| CN111316805A (en) * | 2020-04-14 | 2020-06-23 | 株洲香之优农业科技发展有限责任公司 | Production method of selenium-rich, zinc-rich, cadmium-reducing and yield-increasing high-quality rice and selenium-rich, zinc-rich and high-quality nutritional rice |
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| CN111887250A (en) * | 2020-07-31 | 2020-11-06 | 农业农村部环境保护科研监测所 | Rice leaf surface conditioner and preparation and application methods thereof |
| CN111887250B (en) * | 2020-07-31 | 2021-08-31 | 农业农村部环境保护科研监测所 | Rice leaf surface conditioner and preparation and application methods thereof |
| CN112961003A (en) * | 2021-02-26 | 2021-06-15 | 四川嘉道博文生态科技有限公司 | Base material for reducing cadmium in ligusticum wallichii planting, fertilizer and application thereof |
| CN112961003B (en) * | 2021-02-26 | 2022-07-05 | 四川嘉道博文生态科技有限公司 | Base material for reducing cadmium in ligusticum wallichii planting, fertilizer and application thereof |
| CN113951254A (en) * | 2021-11-17 | 2022-01-21 | 浙江大学 | Nano-type leaf surface regulating agent capable of improving cadmium tolerance of rice and preparation method thereof |
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