CN109704870A - A kind of nucleosides polypeptide amino acid multifunctional bio fertilizer and its preparation method and application - Google Patents
A kind of nucleosides polypeptide amino acid multifunctional bio fertilizer and its preparation method and application Download PDFInfo
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- CN109704870A CN109704870A CN201910162584.5A CN201910162584A CN109704870A CN 109704870 A CN109704870 A CN 109704870A CN 201910162584 A CN201910162584 A CN 201910162584A CN 109704870 A CN109704870 A CN 109704870A
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Abstract
The invention discloses a kind of nucleosides polypeptide amino acid multifunctional bio fertilizer, each components including following mass fraction: 6% ~ 10% compound nucleotide, 6% ~ 10% complex polypeptide, 10% ~ 50% compound amino acid, 1% ~ 5% niacinamide, 2% ~ 5% Zinc vitriol, 2% ~ 15% green vitriol, 1% ~ 6% boric acid, 5% ~ 10% potassium dihydrogen phosphate, 5% ~ 10% surfactant.The invention also discloses the preparation methods of its pulvis and microemulsion.Due to the molecular characterization of its component, with excellent disease-resistant bactericidal activity, it is adjustable growth and development, enhancing photosynthesis and the sorption enhanced function of plant, enhance the disease-resistant, antitoxin of crop, removing toxic substances, degeneration-resistant, cold-resistant, drought resisting, ability resistant to lodging, element needed for supplementing a variety of nutritions and growing promotes crops integrally to grow, strong sprout, healthy tree, precocity, improves single rate, optimize quality, realizes the high yield quality benefit benefit of crops.
Description
Technical field
The present invention relates to bio-fertilizer technical fields, and in particular to a kind of nucleosides polypeptide amino acid multifunctional bio is fertile and its makes
Preparation Method and application.
Background technique
As the improvement of people's living standards, and grain yield be continuously increased, agricultural production is just from single high yield
Change for target to good quality and high output direction.Agricultural product quality especially nutritional quality and people's health quality are increasingly subject to weight
Depending on.It is influenced, is influenced by chemical fertilizer, pesticide Use out of range residual contamination, so that the quality safety of agricultural and sideline product by environmental pollution
It is troubling；Therefore it curbs environmental pollution, improves the resistant to diseases and insects of crop, reduce pesticide, fertilizer amount, become in recent years
One of social hotspots both domestic and external.
There are defects used below for existing agrochemical product: (1) multiselect is with a kind of or several compounded；Dosage
Bigger than normal, utilization rate is relatively low；According to research reports, the effective rate of utilization of agricultural chemicals is lower than 40%, and accelerates the salt of soil
Change, destroys Agricultural Soil Environment；(2) only has amino acid, microelement is applied alone or amino acid adds trace element built；(3)
Plant pest is prevented and treated mostly based on chemical pesticide, it is increasing with the big dosage that pesticide occurs of plant pest, it brings
The remaining potential pollution of chemical pesticide.
High activity organic biological preparation is selected, as high-efficiency multi-function is made in nucleotide, active peptides, amino acid composite preparation
Biological organic fertilizer can be improved crop yield and quality, induce crop anti-adversity, and the harm of disease and pest control reduces pesticide, changes
The dosage of fertilizer, prevents agricultural environment pollution, is able to solve the agricultural product upgrading problem that current agricultural production faces, while ensureing agriculture
The safety of product.
Therefore, studying a kind of can make the fruits such as water fruits and vegetables, the fructification of edible mushroom, the nutrition of wheat, crop kernels
Quality is significantly improved, the new bio-fertilizer that palatability is improved, and to increasing yield and improving quality and ensures that agricultural product security has
Significance, while being also beneficial to making full use of for environmental protection and fertilizer.
Summary of the invention
In view of the deficiency of existing bio-feritlizer, the present invention provides one kind and can supplement multiple nutritional components, have disease-resistant kill
Bacterium activity, regulating crop growth development, the nucleosides polypeptide amino acid multifunctional bio fertilizer for improving crop yield and quality.Specific skill
Art scheme is as follows:
A kind of nucleosides polypeptide amino acid multifunctional bio fertilizer, each component including following mass fraction: 6% ~ 10% compound nucleotide,
6% ~ 10% complex polypeptide, 10% ~ 50% compound amino acid, 1% ~ 5% niacinamide, 2% ~ 5% Zinc vitriol, 2% ~ 15% 7 hydration
Ferrous sulfate, 1% ~ 6% boric acid, 5% ~ 10% potassium dihydrogen phosphate, 5% ~ 10% surfactant.
A kind of nucleosides polypeptide amino acid multifunctional bio fertilizer, including two kinds of dosage forms of pulvis and microemulsion, the pulvis
Active constituent content 30%, appearance granular are in emulsion after being watered；The microemulsion active constituent content 15%, appearance is in brown
Color liquid.
Preferably, the purity of the compound nucleotide is greater than 80%, contains 5'-GMP, 5'-inosinic acid, oligonucleotides.
Preferably, the complex polypeptide is the polymer of poly- bran propylhomoserin, and purity is greater than 80%, wherein respectively by oligomerization
Peptide chain (being less than 20 amino acid units) and middle polymerization peptide chain (20 ~ 40 amino acid units) are formed.
Preferably, the purity of the compound amino acid is greater than 50%, contains glutamic acid, serine, arginine, proline, Soviet Union
Propylhomoserin, asparatate, leucine, isoleucine, valine, alanine, glycine, lysine, phenylalanine, cystine,
Histidine, tyrosine, 6 kinds or more in methionine.
Preferably, the purity of the niacinamide is greater than 96%.
Preferably, the purity of the Zinc vitriol is greater than 98%, and zinc content (in terms of zinc oxide) is greater than 30.0%.
Preferably, the purity of the green vitriol is greater than 98%.
Preferably, the surfactant is polyoxyethylene sorbitan monoleate (Tween 80), two in lauryl sodium sulfate and organosilicon
The mixture of kind composition described above.
Preferably, the surfactant is powdery lauryl sodium sulfate and powdery organosilicon, and the two is by air-flow powder
Broken machining obtains, wherein 90% or more partial size is less than 80 microns.
A kind of preparation method of nucleosides polypeptide amino acid multifunctional bio fertilizer pulvis, comprising the following steps:
Step 1: first that compound nucleotide, complex polypeptide, compound amino acid, niacinamide is first solvable with doubling dose respectively
Property starch after mixing, then with surfactant is uniformly mixed；
Step 2: potassium dihydrogen phosphate is uniformly mixed by Zinc vitriol, green vitriol, boric acid；
Step 3: the material that step 1 and step 2 are mixed is remixed uniformly in blender, nucleosides polypeptide ammonia is become
The pulvis of base acid multifunctional bio fertilizer.
A kind of preparation method of nucleosides polypeptide amino acid multifunctional bio fertilizer microemulsion, comprising the following steps:
Step 1: first that compound nucleotide, complex polypeptide, compound amino acid, niacinamide is first solvable with doubling dose respectively
Property starch after mixing, then with surfactant is uniformly mixed；
Step 2: potassium dihydrogen phosphate is uniformly mixed by Zinc vitriol, green vitriol, boric acid；
Step 3: the material that step 1 and step 2 are mixed is remixed uniformly in blender, nucleosides polypeptide ammonia is become
The pulvis of base acid multifunctional bio fertilizer；
Step 4: step 3 is processed into manufactured nucleosides polypeptide amino acid multifunctional bio fertilizer pulvis in enamel reaction still,
It is added to stirring and dissolving in the pure water measured according to 2 times of pulvis, obtaining the stable colloidal solution of half emulsus is microemulsion.
A kind of nucleosides polypeptide amino acid multifunctional bio is fertile, contained active constituent and content in 30% pulvis are as follows: every 100g
In contain 6 g of polynucleotides, 10 g of polypeptide, 4 g of amino acid 1.
A kind of nucleosides polypeptide amino acid multifunctional bio is fertile, contained active constituent and content in 15% microemulsion are as follows: every
Contain 3 g of polynucleotides, 5 g of polypeptide, 7 g of amino acid in 100g.
Preferably, a kind of nucleosides polypeptide amino acid multifunctional bio fertilizer, each component including following mass fraction: complex nucleus
Thuja acid 6% ~ 20%, poly- bran propylhomoserin polypeptide 5% ~ 10%, in compound amino acid total amino acid content in 10-30% (w/w), effective iron
Content at 2-6% (w/w), the content of effective zinc at 1-5% (w/w), the content of effective boron at 1-5% (w/w), niacinamide
Content 1% ~ 5% (w/w), potassium dihydrogen phosphate 1% ~ 10% (w/w).
The nucleosides polypeptide amino acid multifunctional bio fertilizer is in foliage-spray, root pouring, seed pelleting, soil treatment
Application.
Application of the nucleosides polypeptide amino acid multifunctional bio fertilizer on agricultural garden crop.
Application method: select 30% agricultural nucleosides polypeptide soluble pulvis (15% microemulsion), with clean river water, spring,
Well water etc. dilutes 800 ~ 1200 times (15% 400 ~ 600 times of microemulsions) and carries out foliar spray in crop growth period, can also be using punching
It applies, cave is applied and irrigates use.
Nucleosides polypeptide amino acid multifunctional bio fertilizer provided by the invention has excellent due to the molecular characterization of its component
Disease-resistant bactericidal activity, while also tool induction and the intracorporal growth of activated plant and system of defense strengthen cell and increase, diseases prevention is dispelled
Malicious toxin expelling adjusts growth and development, enhancing photosynthesis and the sorption enhanced function of plant, enhances disease-resistant, antitoxin, the solution of crop
Malicious, degeneration-resistant, cold-resistant, drought resisting, ability resistant to lodging, element needed for supplementing a variety of nutritions and growing promote crops whole
Growth, strong sprout, healthy tree, precocity, improve single rate, and enhancing optimization quality realizes the purpose of the high yield quality benefit benefit of crops.
Compound nucleotide, complex polypeptide used in the present invention (calling agricultural nucleosides polypeptide in the following text) have effects that following:
1, inhibit gene expression viral in crop body, induction plant generates multiple defense reaction, improves the disease resistance of itself
Both it can inhibit gene expression viral in crop body, and controlled its breeding, can also have been acted on by cell activation, and repaired aggrieved plant
Strain damage, while the intracorporal gene expression of plant can be excited again, so that it is generated the chitinase etc. with resistant effect, induces plant
Multiple defense reaction is generated, the disease resistance of itself is improved, plays the role of disease-resistant, insect-prevention.In agricultural nucleosides polypeptide containing carboxyl,
Phenolic hydroxyl group etc. and benzene carboxylic acid's structure, this substance have certain inhibitory anti-virus effect.One of indices of plant: it plants
A kind of inhibition growing plants hormone of ABA (abscisic acid) in object.Under any adverse environmental factor, ABA content can all increase plant.
Abscisic acid is that adversity gene is expressed " first messenger " in starting plant, degeneration-resistant immune system in effective activated plant body.Agriculture
It can be activated the intracorporal synzyme of plant (aldolase, invertase etc.) with nucleosides polypeptide, adjust oxidase active, protect plant growth
Element, this is the fundamental cause for improving stress resistance of plant.
2, to Plant-stimulating activity
(1) stimulation mechanism study
Some agrochemicals men think, more conjugation aromatic structures of agricultural nucleosides polypeptide, the ion exchange knot to-electron acceptor matter
Structure, functional group hydrogen bond association system, all biological effect is had a major impact.It provides part organic nutrition for crop, and adjusts
Nutrient reaches relative balance state in section crop body
(2) agricultural nucleosides polypeptide has the function of auxin
Typical example is compared by the plant hormone of the known actions mechanism such as agricultural nucleosides polypeptide sodium and heteroauxin
What research obtained；With the agricultural nucleosides polypeptide of appropriate amount, the growth of wheat, cotton, tomato, radish can be promoted, there is similar growth
The effect of element, also there is the basic element of cell division.Due to the inhibiting effect to external indoleacetic acid oxidase, so that plant exists
It grows up strong and sturdy under the action of tumor growth element.
(3) agricultural nucleosides polypeptide has the function of similar plants growth hormone, is a kind of natural plants hormone, Neng Gouyou
Effect promotes plant growth.Agricultural nucleosides polypeptide is improved by the activity of some enzymes in stimulation and raising plant by enzymatic activity
The respiratory intensity of plant and the physiological action of root system, achieve the effect that stimulate plant growth: so that blade increased, thickened, protect it is green, under
Portion's leaf senile is postponed, the effect of similar cell mitogen, reduces stomata, transpiration reduces, the effect of similar abscisic acid.
(4) agricultural nucleosides polypeptide can promote seed sprouting, root growth, fruit color early similar to plant endogenous hormones
It is ripe.It is in particular in: promoting the growth and activity of root, similar auxin effect；Seed is promoted to sprout, emergence is neat and seedling is raw
It is long, the effect of similar gibberellin.Promote the effect of cell division and cell elongation, differentiation etc., similar two or more plants
Functions of hormones colours fruit in advance, is mature, similar ethylene dematuration.
3, improve quality of agricultural product
(1) regulate and control enzymatic reaction, enhance plant vital activity
Enzyme is the biocatalyst of plant vital activity, and the effect size of enzyme is showed with the activity of enzyme.Agricultural nucleosides polypeptide energy
The activity for enough stimulating polysaccharase, hydrolyzes young cell wall pectic substance, and softening of the cell wall makes cell be easy to extend division, longitudinal
Cross growth, thus have obvious effect to the growth for promoting the tender cell of cambium children, it is especially aobvious to the effect of the growing point of root
It writes.
Agricultural nucleosides polypeptide can inhibit the activity of auxin oxidase containing a large amount of carboxyls, make the reduction of auxin decomposition rate,
Content improves, to be conducive to root, Stem nematode, keeps crop root of hair fast, and secondary root is more, and root amount increases, and root elongation absorbs crop
Moisture, nutrient ability increase；Cell leakage is improved, nutrient absorption is promoted.Agricultural nucleosides polypeptide can promote sugared invertase, starch
The activity of phosphorylase and some enzymes related with protein, Fatty synthesis, makes sugar, starch, protein, fat, nucleic acid, dimension
Synthesis, cumulative rises of the substances such as raw element, and promote the activity of transferase accelerate various metabolites from cauline leaf or root to fruit
The operating of real and seed, has a direct impact the yield and quality for improving and improve crops；The activity that polysaccharase can also be stimulated, makes
Polysaccharide conversion is Soluble Monosaccharide, to improve fruit sweetness；The ratio of total reducing sugar and nicotine and potassium and chlorine in tobacco leaf is improved, is improved
Quality of tobacco；Improve total sugar content and the content of vitamin in the melons and fruits such as watermelon, apple, citrus.
(2) chlorophyll content is improved, photosynthesis is promoted
Agricultural nucleosides polypeptide can promote the absorption and operating of plant centering microelement, increase the content of leaf chlorophyll significantly
Add, and the activity of protease can be inhibited, slows down chlorophyll decomposition.Agricultural nucleosides polypeptide can improve the work of antioxidase
Property, excessive free radical in Antioxidant Enzyme Systems energy scavenger-cell mitigates destruction of the free radical to chlorophyll.These are all conducive to
Maintenance and raising chlorophyll content, promote photosynthesis, accelerate the accumulation of sugar, promote nucleic acid, chlorophyll, vitamin, amino acid
Synthesis, improve plant quality and trophic level.
(3) enhance respiration
Since agricultural nucleosides peptide molecule is smaller, it is easy to enter plant cell, as the donor of plant polyphenol or the receptor of hydrogen,
The oxidation-reduction process of plant is directly affected, promotes the synthesis of adenosine tripho hate (ATP), plays respiratory catalyst；Agricultural core
Glycosides polypeptide can also enhance the activity of respiratory enzyme, especially promote the activity of terminal oxidase, and enhance respiration.Respiration
Qiang Liao, to promote absorption function and the substance synthesis of root.
(4) promote the absorption and transport of mineral element
Many trace quantity mineral substance elements such as Fe, Cu, Zn, Mn, B, Mo etc. are the composition portions for participating in the movable enzyme of plant metabolism or coenzyme
Point, can activity to a variety of enzymes and stress resistance of plant have a major impact, have plenty of the constituent of eucaryotic cell structure substance.Agricultural core
The active group (including carboxyl, hydroxyl and certain groups containing P, O, N, S) of glycosides polypeptide is typically all electronq donor, is held very much
Easily complex, referred to as complex compound or chela are constituted with many electron acceptors (polyvalent metal ion, organic group or ion)
Close object.For example, agricultural nucleosides polypeptide-Zn, agricultural nucleosides polypeptide-Mn, agricultural nucleosides polypeptide-Fe, etc., practical is largely complexing
(chelating) object.Furthermore agricultural nucleosides polypeptide also has ion exchange property, the active hydrogen ion (H on carboxyl and phenolic hydroxyl group+) very
It is easy by monovalent cation (K+、Na+、Li+、NH4 +) and part bivalent metal ion (Ca2+、Mg2+、Fe2+Deng) displacement, form agriculture
With nucleosides polypeptide salt etc..This effect of agricultural nucleosides polypeptide improves absorption of the crop to many microelements.
4, enhance crop anti-adversity
(1) cold-resistant mechanism
The content of abscisic acid in plant can be improved in agricultural nucleosides polypeptide, and abscisic acid can be such that proline content increases, and proline is made
Stablize structure of biological macromolecule for important osmotic field mass-energy, so that freeze point depression is had frost protective effect, to improve plant
The cold hardiness of object.
(2) anti-disease mechanism
The agricultural antibacterial anti-disease mechanism research of nucleosides polypeptide: containing in agricultural nucleosides polypeptide there are many nucleotide and amino acids substance,
It can generate effective immunity to the certain diseases of plant, contain 3 kinds of nucleotide, 17 kinds of amino acid in agricultural nucleosides polypeptide, this
A little substances are the Plant elicitors with different inductive effects.Agricultural nucleosides polypeptide make aerophile bacterium in soil, actinomyces,
The quantity increase of cellulose-decomposing bacteria is more, significantly reduces the quantity of fungi, the ratio of bacterium and fungi number significantly improves, and can prevent
It controls much by fungus-caused disease.To the rot disease of various crop, soft rot, root rot, canker, brown spot, shot hole,
It is downy mildew, gray mold, wilt disease, verticillium wilt, leaf blight, damping-off, leaf spot, banded sclerotial blight, blight dis-ease, anthracnose, epidemic disease, white
The diseases such as powder disease, sclerotiniose, virosis and rust, samping off, rice blast, seedling rot have excellent control efficiency.
Nucleosides polypeptide amino acid multifunctional bio fertilizer provided by the invention, has below compared with prior art beneficial to effect
Fruit:
1. of the invention by micromolecule active polypeptide, three kinds of nucleotide, six kinds of poly- propylhomoserin peptides and 16 kinds of organic knots of amino acid science
It closes, each functional component interaction, cooperative compensating, scientific compatibility enhance this product effect rapidly, have the stimulation healthy and strong life of plant
Long function supplements plant zinc, iron, boron microelement function, changes and improves crop protein, sugar, amino acid and edible flavor
Function, induction plant generate cold-resistant, disease-resistant, pest-resistant, mitigation phytotoxicity function；
2. advanced synthesis processing technology provided by the invention ensures that the bioactivity of agricultural nucleosides polypeptide, absorption are more preferable, rises
Effect is faster, the lasting period is longer, better effect；
3. nucleosides polypeptide amino acid multifunctional bio fertilizer provided by the invention, is adjusted induction Genes For Plant Tolerance disease pest ability, improves and plant
The immune function of object enhances photosynthesis, improves crop anti-adversity, improves fruit-setting rate, reduces fruit drop, improves the product of crop
Matter, the flavor for improving fruit, increase the nutritive value of fruit, keep fruit colour bright, increasing both production and income can be in apple, grape, sweet tea
Promoting on the crops such as orange, citrus, cucumber, watermelon, muskmelon, cucurbita pepo, tomato, capsicum, edible mushroom, Chinese cabbage, radish, tobacco makes
With.
Specific embodiment
Further to illustrate technological means and its effect adopted by the present invention, below in conjunction with preferred implementation of the invention
Example is described in detail.
Embodiment 1
A kind of nucleosides polypeptide amino acid multifunctional bio fertilizer, each component including following mass fraction: 6% ~ 10% compound nucleotide,
6% ~ 10% complex polypeptide, 10% ~ 50% compound amino acid, 1% ~ 5% niacinamide, 2% ~ 5% Zinc vitriol, 2% ~ 15% 7 hydration
Ferrous sulfate, 1% ~ 6% boric acid, 5% ~ 10% potassium dihydrogen phosphate, 5% ~ 10% surfactant.
Preferably, the purity of the compound nucleotide is greater than 80%, contains 5'-GMP, 5'-inosinic acid, oligonucleotides.
Preferably, the complex polypeptide is the polymer of poly- bran propylhomoserin, and purity is greater than 80%, wherein respectively by oligomerization
Peptide chain (being less than 20 amino acid units) and middle polymerization peptide chain (20 ~ 40 amino acid units) are formed.
Preferably, the purity of the compound amino acid is greater than 50%, contains glutamic acid, serine, arginine, proline, Soviet Union
Propylhomoserin, asparatate.
Preferably, the purity of the niacinamide is greater than 96%.
Preferably, the purity of the Zinc vitriol is greater than 98%, and zinc content (in terms of zinc oxide) is greater than 30.0%.
Preferably, the purity of the green vitriol is greater than 98%.
Preferably, the surfactant is two or more groups in polyoxyethylene sorbitan monoleate, lauryl sodium sulfate and organosilicon
At mixture.
Preferably, the surfactant is powdery lauryl sodium sulfate and powdery organosilicon, and the two is by air-flow powder
Broken machining obtains, wherein 90% or more partial size is less than 80 microns.
A kind of preparation method of nucleosides polypeptide amino acid multifunctional bio fertilizer pulvis, comprising the following steps:
Step 1: first that compound nucleotide, complex polypeptide, compound amino acid, niacinamide is first solvable with doubling dose respectively
Property starch after mixing, then with surfactant is uniformly mixed；
Step 2: potassium dihydrogen phosphate is uniformly mixed by Zinc vitriol, green vitriol, boric acid；
Step 3: the material that step 1 and step 2 are mixed is remixed uniformly in blender, nucleosides polypeptide ammonia is become
The pulvis of base acid multifunctional bio fertilizer.
The nucleosides polypeptide amino acid multifunctional bio fertilizer pulvis well water being prepared according to the above method is diluted 1000 times
Periodical foliage-spray is carried out in the apple development phase, not spray the apple tree of foliar fertilizer as compareing, the results showed that spray this
Compared with the control, the chlorophyll content of leaf increases by 25 ~ 35%, rate of photosynthisis and improves the increasing of 15 ~ 25%, yield the apple tree of bio-fertilizer
Total sugar content in 20 ~ 30%, fruit is added to improve 10 ~ 25%, Vitamin C content increases by 25 ~ 40%, malic acid content and declines, fruit
Color area is big, and color is also more bright-coloured.
Embodiment 2
A kind of nucleosides polypeptide amino acid multifunctional bio fertilizer, each component including following mass fraction: 6% ~ 10% compound nucleotide,
6% ~ 10% complex polypeptide, 10% ~ 50% compound amino acid, 1% ~ 5% niacinamide, 2% ~ 5% Zinc vitriol, 2% ~ 15% 7 hydration
Ferrous sulfate, 1% ~ 6% boric acid, 5% ~ 10% potassium dihydrogen phosphate, 5% ~ 10% surfactant.
Preferably, the purity of the compound nucleotide is greater than 80%, contains 5'-GMP, 5'-inosinic acid, oligonucleotides.
Preferably, the complex polypeptide is the polymer of poly- bran propylhomoserin, and purity is greater than 80%, wherein respectively by oligomerization
Peptide chain (being less than 20 amino acid units) and middle polymerization peptide chain (20 ~ 40 amino acid units) are formed.
Preferably, the purity of the compound amino acid is greater than 50%, contains glutamic acid, serine, arginine, proline, Soviet Union
Propylhomoserin, asparatate, leucine, isoleucine, valine, alanine, glycine, lysine.
Preferably, the purity of the niacinamide is greater than 96%.
Preferably, the purity of the Zinc vitriol is greater than 98%, and zinc content (in terms of zinc oxide) is greater than 30.0%.
Preferably, the purity of the green vitriol is greater than 98%.
Preferably, the surfactant is two or more groups in polyoxyethylene sorbitan monoleate, lauryl sodium sulfate and organosilicon
At mixture.
Preferably, the surfactant is powdery lauryl sodium sulfate and powdery organosilicon, and the two is by air-flow powder
Broken machining obtains, wherein 90% or more partial size is less than 80 microns.
A kind of preparation method of nucleosides polypeptide amino acid multifunctional bio fertilizer pulvis, comprising the following steps:
Step 1: first that compound nucleotide, complex polypeptide, compound amino acid, niacinamide is first solvable with doubling dose respectively
Property starch after mixing, then with surfactant is uniformly mixed；
Step 2: potassium dihydrogen phosphate is uniformly mixed by Zinc vitriol, green vitriol, boric acid；
Step 3: the material that step 1 and step 2 are mixed is remixed uniformly in blender, nucleosides polypeptide ammonia is become
The pulvis of base acid multifunctional bio fertilizer.
A kind of preparation method of nucleosides polypeptide amino acid multifunctional bio fertilizer microemulsion, comprising the following steps:
Step 1: first that compound nucleotide, complex polypeptide, compound amino acid, niacinamide is first solvable with doubling dose respectively
Property starch after mixing, then with surfactant is uniformly mixed；
Step 2: potassium dihydrogen phosphate is uniformly mixed by Zinc vitriol, green vitriol, boric acid；
Step 3: the material that step 1 and step 2 are mixed is remixed uniformly in blender, nucleosides polypeptide ammonia is become
The pulvis of base acid multifunctional bio fertilizer；
Step 4: step 3 is processed into manufactured nucleosides polypeptide amino acid multifunctional bio fertilizer pulvis in enamel reaction still,
It is added to stirring and dissolving in the pure water measured according to 2 times of pulvis, obtaining the stable colloidal solution of half emulsus is microemulsion.
The clean river water of nucleosides polypeptide amino acid multifunctional bio fertilizer microemulsion being prepared according to the above method is diluted
500 times carry out periodical dropper rhizosphere in the chili growth phase, to apply the capsicum of common fertilizer as control, the results showed that using being somebody's turn to do
The capsicum of bio-fertilizer dropper rhizosphere compared with the control, the single plant fruiting number of capsicum increase by 16.7 ~ 33.3%, single fruit weight increase by 2.2 ~
14.7%, yield increases by 20.1 ~ 34.5%, and capsicum pest and disease damage reduces 23 ~ 36%, and color is vivider.
Embodiment 3
A kind of nucleosides polypeptide amino acid multifunctional bio fertilizer, each component including following mass fraction: 6% ~ 10% compound nucleotide,
6% ~ 10% complex polypeptide, 10% ~ 50% compound amino acid, 1% ~ 5% niacinamide, 2% ~ 5% Zinc vitriol, 2% ~ 15% 7 hydration
Ferrous sulfate, 1% ~ 6% boric acid, 5% ~ 10% potassium dihydrogen phosphate, 5% ~ 10% surfactant.
Preferably, the purity of the compound nucleotide is greater than 80%, contains 5'-GMP, 5'-inosinic acid, oligonucleotides.
Preferably, the complex polypeptide is the polymer of poly- bran propylhomoserin, and purity is greater than 80%, wherein respectively by oligomerization
Peptide chain (being less than 20 amino acid units) and middle polymerization peptide chain (20 ~ 40 amino acid units) are formed.
Preferably, the purity of the compound amino acid is greater than 50%, contains glutamic acid, serine, arginine, proline, Soviet Union
Propylhomoserin, asparatate, leucine, isoleucine, valine, alanine, glycine, lysine, phenylalanine, cystine,
Histidine, tyrosine, methionine.
Preferably, the purity of the niacinamide is greater than 96%.
Preferably, the purity of the Zinc vitriol is greater than 98%, and zinc content (in terms of zinc oxide) is greater than 30.0%.
Preferably, the purity of the green vitriol is greater than 98%.
Preferably, the surfactant is two or more groups in polyoxyethylene sorbitan monoleate, lauryl sodium sulfate and organosilicon
At mixture.
Preferably, the surfactant is powdery lauryl sodium sulfate and powdery organosilicon, and the two is by air-flow powder
Broken machining obtains, wherein 90% or more partial size is less than 80 microns.
A kind of preparation method of nucleosides polypeptide amino acid multifunctional bio fertilizer pulvis, comprising the following steps:
Step 1: first that compound nucleotide, complex polypeptide, compound amino acid, niacinamide is first solvable with doubling dose respectively
Property starch after mixing, then with surfactant is uniformly mixed；
Step 2: potassium dihydrogen phosphate is uniformly mixed by Zinc vitriol, green vitriol, boric acid；
Step 3: the material that step 1 and step 2 are mixed is remixed uniformly in blender, nucleosides polypeptide ammonia is become
The pulvis of base acid multifunctional bio fertilizer.
The nucleosides polypeptide amino acid multifunctional bio fertilizer microemulsion spring being prepared according to the above method is diluted 500 times
Be added in tobacco seed coating agent and tobacco seed be coated, using be not added with the coating tobacco seed of the bio-fertilizer as pair
According to, the results showed that add the bio-fertilizer coating tobacco seed emergence rate improve 12.3 ~ 29.4%, radical increase by 15.4 ~ 26.7%,
Yield of tobacco increases by 10.4 ~ 18.2%, and perfume quantity increases by 12.9 ~ 21.8%, and disease incidence reduces by 3.2 ~ 21.5%.
Long-term test results show nucleosides polypeptide amino acid multifunctional bio fertilizer provided by the invention using foliage-spray,
The application of the various ways such as root pouring, seed pelleting, soil treatment can obtain good effect, not only prevent and treat virosis effect
It protrudes and also has preferable control effect to some fungal diseases often sent out, while the quality of crop can also be improved, changed
The flavor of good fruit reality, have additional nutrients value, can make the fruits such as water fruits and vegetables, the fructification of edible mushroom, wheat, crop kernels
Zinc, iron obtain high efficiency bio-augmentation, and nutritional quality is significantly improved, and palatability is improved.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, ability
Other modifications or equivalent replacement that domain those of ordinary skill makes technical solution of the present invention, without departing from skill of the present invention
The spirit and scope of art scheme, are intended to be within the scope of the claims of the invention.
Claims (10)
1. a kind of nucleosides polypeptide amino acid multifunctional bio fertilizer, which is characterized in that each component including following mass fraction: 6% ~
10% compound nucleotide, 6% ~ 10% complex polypeptide, 10% ~ 50% compound amino acid, 1% ~ 5% niacinamide, 2% ~ 5% 7 hydrated sulfuric acid
Zinc, 2% ~ 15% green vitriol, 1% ~ 6% boric acid, 5% ~ 10% potassium dihydrogen phosphate, 5% ~ 10% surfactant.
2. nucleosides polypeptide amino acid multifunctional bio fertilizer according to claim 1, which is characterized in that including pulvis and micro emulsion
Agent, the pulvis active constituent content 30%, appearance granular are in emulsion after being watered；The microemulsion active constituent content
15%, the brown liquid of appearance.
3. nucleosides polypeptide amino acid multifunctional bio fertilizer according to claim 1, which is characterized in that the compound nucleotide
Purity be greater than 80%, contain 5'-GMP, 5'-inosinic acid, oligonucleotides.
4. nucleosides polypeptide amino acid multifunctional bio according to claim 1 fertilizer, which is characterized in that the complex polypeptide is
The polymer of poly- bran propylhomoserin, purity are greater than 80%, wherein respectively by less than 20 amino acid units oligomerization peptide chain and 20 ~
The middle polymerization peptide chain of 40 amino acid units is formed.
5. nucleosides polypeptide amino acid multifunctional bio fertilizer according to claim 1, which is characterized in that the compound amino acid
Purity be greater than 50%, contain glutamic acid, serine, arginine, proline, threonine, asparatate, leucine, different bright ammonia
Acid, valine, alanine, glycine, lysine, phenylalanine, cystine, histidine, tyrosine, 6 kinds in methionine with
On.
6. nucleosides polypeptide amino acid multifunctional bio fertilizer according to claim 1, which is characterized in that the niacinamide
Purity is greater than 96%；The purity of the Zinc vitriol is greater than 98%, and zinc content (in terms of zinc oxide) is greater than 30.0%；Described seven
The purity of ferrous sulfate hydrate is greater than 98%.
7. nucleosides polypeptide amino acid multifunctional bio fertilizer according to claim 1, which is characterized in that the surfactant
For the mixture of two or more compositions in polyoxyethylene sorbitan monoleate, lauryl sodium sulfate and organosilicon；The surfactant is powder
Shape lauryl sodium sulfate and powdery organosilicon, the two are machined to obtain by air-flow crushing, wherein 90% or more partial size is less than 80
Micron.
8. the preparation method of nucleosides polypeptide amino acid multifunctional bio fertilizer pulvis according to claim 2, which is characterized in that
The following steps are included:
Step 1: first that compound nucleotide, complex polypeptide, compound amino acid, niacinamide is first solvable with doubling dose respectively
Property starch after mixing, then with surfactant is uniformly mixed；
Step 2: potassium dihydrogen phosphate is uniformly mixed by Zinc vitriol, green vitriol, boric acid；
Step 3: the material that step 1 and step 2 are mixed is remixed uniformly in blender, nucleosides polypeptide ammonia is become
The pulvis of base acid multifunctional bio fertilizer.
9. the preparation method of nucleosides polypeptide amino acid multifunctional bio fertilizer microemulsion according to claim 2, feature exist
In, comprising the following steps:
Step 1: first that compound nucleotide, complex polypeptide, compound amino acid, niacinamide is first solvable with doubling dose respectively
Property starch after mixing, then with surfactant is uniformly mixed；
Step 2: potassium dihydrogen phosphate is uniformly mixed by Zinc vitriol, green vitriol, boric acid；
Step 3: the material that step 1 and step 2 are mixed is remixed uniformly in blender, nucleosides polypeptide ammonia is become
The pulvis of base acid multifunctional bio fertilizer；
Step 4: step 3 is processed into manufactured nucleosides polypeptide amino acid multifunctional bio fertilizer pulvis in enamel reaction still,
It is added to stirring and dissolving in the pure water measured according to 2 times of pulvis, obtaining the stable colloidal solution of half emulsus is microemulsion.
10. application of any nucleosides polypeptide amino acid multifunctional bio fertilizer on agricultural garden crop according to claim 1 ~ 9.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111943754A (en) * | 2020-08-18 | 2020-11-17 | 山东农业大学 | Crop straw biochar composite microbial organic fertilizer and preparation method and application thereof |
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