CN106238445A - A kind of method utilizing EDTA and brassin lactone combined induction rye grass remediating lead-contaminated soil - Google Patents
A kind of method utilizing EDTA and brassin lactone combined induction rye grass remediating lead-contaminated soil Download PDFInfo
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- CN106238445A CN106238445A CN201610540178.4A CN201610540178A CN106238445A CN 106238445 A CN106238445 A CN 106238445A CN 201610540178 A CN201610540178 A CN 201610540178A CN 106238445 A CN106238445 A CN 106238445A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/08—Reclamation of contaminated soil chemically
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
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- Engineering & Computer Science (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
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Abstract
The invention belongs to heavy metal-polluted soil and repair field, disclose a kind of method utilizing EDTA and brassin lactone combined induction rye grass remediating lead-contaminated soil.The method is by planting after English ryegrass in by the soil of lead contamination, periodically applies EDTA and the nutritional solution of periodically foliage spray plant growth regulation brassin lactones in soil, after trophophase terminates, cradles rye grass overground part.Hereafter, according to actual soil regime needs, utilize the feature that English ryegrass can repeatedly cradle, carry out taking turns cultivation plantation more, thus reach the purpose of remediating heavy metal lead-contaminated soil.The method promotes the growth of rye grass, solves the problems such as the biomass caused with abandoned mine lead-contaminated soil suppression Ryegrass Growth reduces, blade is tiny, has the advantages such as absorption efficiency is high, enrichment degree is high, repairing effect is notable.
Description
Technical field
The invention belongs to heavy metal-polluted soil and repair field, be specifically related to one and utilize chelating agen and plant growth regulator connection
The method closing induction rye grass remediating lead-contaminated soil.
Background technology
" the whole nation Soil Pollution Investigation publication " display issued according to Environmental Protection Departments in 2014 and Ministry of Land and Resources:
In 1672 soil point positions in 70 mining areas of investigation, super punctuation bit accounts for 33.4%, and major pollutants are cadmium, lead, arsenic and multi-ring
Aromatic hydrocarbons；Non-ferrous metal mining area Soil Surrounding cadmium, arsenic, lead etc. pollute the most serious；At 81 pieces of Industrial Wasteland of investigation 775
In soil point position, super punctuation bit accounts for 34.9%, and major pollutants are zinc, hydrargyrum, lead, arsenic and polycyclic aromatic hydrocarbon, relate generally to chemical industry,
The industry such as mining industry, Metallurgy Industry.Whole nation soil environment situation totally allows of no optimist, and discards ground soil environment outstanding problem industrial and mineral.
Find economy, environmental protection and effective measures restoration of soil polluted by heavy metal and become hot research problem.
Lead (Pb), as the stable element that quality is maximum, once entering environment cannot degrade again, can keep it for a long time
Toxicity.Research shows, the excess Pb reproductive system etc. to nervus centralis, red blood cell, kidney, cardiovascular and the men and women of human body
The toxic effect of multiple organ.For child, even if lead concentration keeps acceptable level in blood, still have influence on child
Intelligent development and expression behaviour abnormal.Additionally, lead can also make people produce and have a stomach-ache by affecting enzyme and cellular metabolism, have a headache, quiver
Tremble irritated with nerve, under serious conditions, until dead.Industry and mining to be developed are discarded in the middle of ground, and lead contamination is the tightest
Weight, to make these discarded becomes utilizable commercial land, the residential land that even suitable people lives, needs low one-tenth badly
Originally, efficient soil remediation measure.
Ethylenediaminetetraacetic acid (EDTA), is a kind of important chelating agent, can be with complexation heavy metal ion and separation metal.By
In it, there is the performance of activation heavy metal, stable state metal can be converted into the metal of free state and EDTA complex, therefore, it
It is commonly used in the fields such as soil chemistry drip washing reparation and phytoremediation contaminated soil.
In recent years, many researchers utilize plant growth regulator, respectively by sides such as plant seed soaking, foliage-sprays
Formula repairing polluted soil.Plant growth regulator is broadly divided into 5 big classes: auxin, gibberellins, the basic element of cell division, abscisic acid and
Ethylene.And brassin lactones (Brassinolide, BR) was studied through more than ten years of scientist, it was demonstrated that it is different from known five big
One Plant Hormone of hormone, as far back as 1987, just is known as being the 6th class phytohormone by academia.BR is efficient as one
Phytohormone, mainly has the effect of the following aspects: (1) promotes cell division, increases fruit；(2) Delaying Leaf-Senescence, promotees
Enter paddle permanent and protect green；(3) promote lateral bud growth, improve crop yield；(4) improve crop quality, increase commercial value.
Rye grass is due to features such as itself growth weather conditions are broad and can repeatedly cradle, thus is extensively introduced a fine variety as excellent
The aspects such as good herbage, feedstuff and lawn.Under the conditions of the different Lead sweet of Yang Mingyan research, the enriched character of lead is sent out by rye grass
Existing, rye grass is time in contaminated soil, lead content is less than 1000mg/kg, and its overground part dry weight does not has significant change, rye (Secale cereale L.) is described
Grass has preferable patience to lead.Thus, rye grass is widely used in heavy-metal contaminated soil phytoremediation field.
Plant to the accumulation ability of heavy metal-polluted soil except with plant itself to the enrichment of heavy metal-polluted soil and transfer ability
Outside the Pass having, also it is heavily dependent on the biological effectiveness of heavy metal-polluted soil.Many researchs are by applying chelating agen toward soil
Improving the activity of heavy metal, improve the utilizability of heavy metal-polluted soil, the fortification of plants absorption to heavy metal-polluted soil, raising is planted
Especially overground part heavy metal concentration in object.But, chelation evoked under the conditions of a large amount of accumulation of plant heavy metal of body also carry
The high coercion of heavy metal on plants growth, may affect the normal growth of plant, cause the reduction of phytomass, support
The part that disappeared increases, because of heavy metal concentration, the strengthening effect produced, thus defines plant heavy metal of body concentration and plant biological
Growth and decline relation between amount.Based on chelating agen to the activation of heavy metal-polluted soil and phytohormone to plant Heavy Metal Tolerance with
The facilitation of accumulation, uses exogenous heavy metal chelating agen associating phytohormone jointly to induce phytoremediation heavy metal polluted soil
Earth, growth and decline relation between heavy metal concentration in regulation and control phytomass and plant, thus improve phytoremediation efficiency, it is one
The research direction of individual worth exploration.
The present invention, on the basis of applicant studies for many years, discloses one and utilizes EDTA and brassin lactone combined induction
The method of rye grass remediating lead-contaminated soil.
Summary of the invention
The invention provides EDTA and the plant of brassin lactone combined induction rye grass of a kind of mining area lead-contaminated soil
Restorative procedure, solves the plant caused with the high lead content soil suppression Ryegrass Growth of lead ore Qu Wei representative short and small, raw
Thing amount is low, yellow leaf and the speed of growth problem such as slowly.Have remediation efficiency height, repairing efficiency is short, repairing effect is notable
And the feature such as can repeatedly cradle.
The present invention is a kind of method improving lead-contaminated soil phytoremediation efficiency, and concrete technical scheme is as follows: first exist
The soil of lead contamination is planted rye grass, manages rye grass, regular foliage spray during Ryegrass Growth in conjunction with agronomic measures
External source brassin lactones and to plantation soil in apply a small amount of chelating agen EDTA, disposably cut after rye grass maturation
Cut and focus on, such as, as solid waste burning or carry out the heavy metal lead of specialty and reclaim, completing the phytoremediation cycle is 2
Month.After plant harvest, next round rye grass cultivation can be carried out as required.
Described rye grass selects English ryegrass.
Described agronomic measures refers to water, illumination, temperature control etc..
Described regular foliage spray external source brassin lactones is to spray once every 10 days after rye grass germinates.
Described regular apply in soil a small amount of chelating agen EDTA be after rye grass germinates the 30th day and the 45th day to soil
Earth applies low-concentration EDTA solution.
Preferably, the consumption of brassin lactones is 5～10mL 150 strains, and content is 0.5～1mg/L.
Preferably, the EDTA consumption of low concentration is 100～200mL kg-1, content is 1.25～2.5mmol kg-1。
In the present invention, the Main Function of rye grass is can be with the higher lead-contaminated soil of resistance to concentration with can repeatedly cut
Cut.
In the present invention, the Main Function of brassin lactones is to delay ryegrass leaves old and feeble, promotes paddle permanent to protect green；Promote
Enter plant cell division, improve the upperground part biomass；Strengthening the rye grass patience to mining area high-concentration lead, promote underground part lead to
Overground part shifts, thus improves rye grass and repair the efficiency of lead.
In the present invention, the Main Function of low-concentration EDTA is to enter lead-contaminated soil by the form of solution, promotes stable
State lead changes to free state and complex state, improves the activity of heavy metal so that lead is more conducive to rye grass and absorbs.
Beneficial effects of the present invention:
The plant growth regulator brassin lactones of foliage spray after plantation can promote Ryegrass Growth, alleviates rye grass
The toxicity of heavy metal of body lead, strengthens the rye grass patience to high-concentration lead, thus improves rye grass within the short cycle to lead
Absorption efficiency, possess good application prospect in phytoremediation field；The EDTA of low dosage coordinates appropriate nonhazardous effect
Growth regulator brassin lactones foliage-spray is under conditions of ensureing that lead-contaminated soil area rye grass grows the most for a long time, same
Step improves the rye grass overground part extraction efficiency to lead；Additionally, utilize the feature that rye grass is gathered in continuously, it is achieved that to mining area
The continuous repair of lead-contaminated soil, also avoids EDTA to be excessively used the potential secondary pollution risk brought simultaneously.
Accompanying drawing explanation
Fig. 1 different disposal is on rye grass overground part and the impact of underground part dry weight
In figure: M 500mg/kgPb contaminated soil；B1 foliage-spray 0.025mg/LBR；B2 foliage-spray
0.05mg/L BR；B3 foliage-spray 0.1mg/L BR；E individually applies 2.5mmol/kg EDTA in soil；EB1 soil
Execute 2.5mmol/kg EDTA+ foliage-spray 0.025mg/L BR；EB2 soil executes 2.5mmol/kg EDTA+ foliage-spray
0.05mg/LBR；EB3 soil executes 2.5mmol/kgEDTA+ foliage-spray 0.1mg/LBR.
Fig. 2: different disposal is on rye grass overground part and the impact of underground part lead content
Detailed description of the invention
Below in conjunction with instantiation, the present invention is further illustrated.
Embodiment 1
1 test plant
Select English ryegrass.
2 for examination soil
For examination soil from periphery vegetable plot, science and education city, Changzhou, its basic physical and chemical (background value) it is: pH value is 7.2, has
Machine matter content is 7.48g/kg, available nitrogen, rapid available phosphorus, available potassium and soil total P b content is respectively as follows: 35.54,24.77,
121.42 and 30.62mg/kg.
3 pot experiments
Heavy metal Pb is with analytical pure Pb (NO3)2Solution form sprays application to soil, fully mixes through blender, balances 6
Month, simulation mining area Pb contaminated soil (Pb content is 500mg/kg), with original clean soil as comparison.Each plastic tub
25cm (diameter) × 17cm (high) dress soil 2.5kg.Seed is sterilized 30min through 4%NaClO, and sows after cleaning with deionized water,
Every 250, basin (to ensure that after germinateing, every basin is more than 150 strains).Greenhouse day/night temperatures is 25/15 DEG C, and every day waters
200mL, to keep soil moisture content 70% as maximum water-holding capacity.According to following scheme, every 10 days leaves after germination
Face sprays 5mL brassin lactones, within the 30th day and the 45th day, applies 100mL EDTA in soil, gathers in the crops rye grass after 60 days.
Process:
M processes 500mg/kg Pb contaminated soil (comparison).
B1 processes foliage-spray 0.025mg/L BR.
B2 processes foliage-spray 0.05mg/L BR.
B3 processes foliage-spray 0.1mg/L BR.
E processes and individually applies 2.5mmol/kg EDTA in soil.
EB1 processes soil and executes 2.5mmol/kg EDTA+ foliage-spray 0.025mg/L BR.
EB2 processes soil and executes 2.5mmol/kg EDTA+ foliage-spray 0.05mg/L BR.
EB3 processes soil and executes 2.5mmol/kg EDTA+ foliage-spray 0.1mg/L BR.
4 rye grass each organ dry weight measures
After plant being rinsed well with tap water, with the EDTA-Na of 20mmol/L2Soak 30min, remove root system surface and inhale
Attached Pb, then rinse 3～4 times with tap water, finally use deionized water rinse, blot surface moisture, be divided into overground part and underground
Portion, complete at 105 DEG C 0.5h, dries to constant weight, weighs overground part and underground part dry weight with electronic balance for 75 DEG C.
5 rye grass overground parts and underground part Determination of Pb
When measuring heavy metal Pb, weigh 200mg and pulverize drying sample, add 65%HNO38mL and 30%H2O21mL, uses
Microwave dissolver (the new instrument in Shanghai, MDS-8G) is cleared up, and the various tenor of digestion solution uses atomic absorption spectrophotometer
(Jena, Germany novAA300) measures and is converted into plant shoot and the content of underground part per dry wt.
Test 8 process of design altogether, each process repeats for 3 times, and its result is as shown in Table 1 and Table 2.
Table 1 different disposal is on rye grass overground part and the impact of underground part dry weight
This example is implemented to show, relative M process, independent foliage-spray brassin lactones improves rye grass in varying degrees
Overground part and underground part dry weight；And individually applying EDTA, rye grass overground part and underground part dry weight do not reduce；And brassin
Lactone is mixed with EDTA to be executed, and rye grass each organ dry weight does not has significant change.
Table 2 different disposal is on rye grass overground part and the impact of underground part lead content
This example is implemented to show, singly executes certain density brassin lactones and is promoting rye grass overground part and underground part lead
While content, promote lead and shift to overground part, but the most notable；Individually apply EDTA, significantly improve rye grass overground part
With underground part lead content, 4.70 and 1.89 times of respectively M process；Along with the rising of brassin lactones concentration, it is joined with EDTA
Execute and significantly improve overground part lead content, promote lead and shifted to overground part by underground part, substantially increase the repairing effect of lead.
To sum up, this example is implemented to show, after EDTA and brassin lactones are joined and executed, is ensureing because EDTA is manured into soil rye (Secale cereale L.)
Under conditions of grass dry weight does not has significant change, EB1, EB2 and EB3 overground part lead content significantly improves 171%, 528% respectively
With 655% (relative M).Therefore, in conjunction with above feature, the present invention utilizes the feature that rye grass can repeatedly cradle simultaneously, it is achieved that
Environment-friendly type reparation continuously to mining area lead-contaminated soil.
Claims (6)
1. the method utilizing EDTA and brassin lactone combined induction rye grass remediating lead-contaminated soil, it is characterised in that bag
Include following steps: in by the soil of lead contamination, first plant English ryegrass, in combination with multiple agronomic measures to for many years
Raw rye grass carries out Cultivate administration, then periodically applies ethylenediaminetetraacetic acid (EDTA) and foliage-spray brassin in soil
The nutritional solution of lactone, cradles its overground part after English ryegrass maturation, and the rye grass of results is by focusing on
Or as Renewable resource extraction and application.
Method the most according to claim 1, the plant repaired is: English ryegrass.
Method the most according to claim 1, the chelating agen repaired is combined as with growth regulator: EDTA and brassin
Lactone.
Method the most according to claim 1, in soil apply every kilogram of soil of external source chelating agen in apply 1.25～
The every 1000mL of external source plant growth regulator of 2.5mmol EDTA and foliage-spray contains the brassin of 0.5～0.1mg
Lactone.
Method the most according to claim 1, after plant germination, carried out periodically sprinkle external source every 10 days to plant leaf surface
Brassin lactones, sprays 5～10mL/150 strains every time；The 30th day and the 45th day after germination uniformly applies 100 in soil
～200mL external source chelating agen EDTA.
Method the most according to claim 1, disposably cradles English ryegrass overground part and focuses on, example
As solid waste burning or carry out specialty heavy metal lead reclaim.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107671112A (en) * | 2017-09-11 | 2018-02-09 | 甘肃省地质矿产勘查开发局第三地质矿产勘查院 | Wild plant restoration method for heavy metal contaminated soil |
| CN108393338A (en) * | 2017-09-22 | 2018-08-14 | 河南科技大学 | A kind of method and application strengthened rye grass and repair metal strontium polluted soil |
| CN108636994A (en) * | 2016-11-07 | 2018-10-12 | 保定学院 | A kind of Copper Mine Abandoned Place Pb in Soil zinc heavy metal pollution restorative procedure |
| CN109317512A (en) * | 2018-09-29 | 2019-02-12 | 常州大学 | A kind of method that EDTA strengthens asparagus remediating lead-contaminated soil |
| CN110523774A (en) * | 2019-09-30 | 2019-12-03 | 武汉工程大学 | The method for discarding lead contamination in ground using the plant combined removal phosphorus ore of indigenous microorganism- |
| CN113909282A (en) * | 2021-10-29 | 2022-01-11 | 南京大学 | Plant management method for heavy metal contaminated soil |
| CN115152542A (en) * | 2022-05-24 | 2022-10-11 | 四川农业大学 | Application method of brassinolide leaf surface blocking and controlling agent in Chinese cabbage planting |
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Cited By (8)
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|---|---|---|---|---|
| CN108636994A (en) * | 2016-11-07 | 2018-10-12 | 保定学院 | A kind of Copper Mine Abandoned Place Pb in Soil zinc heavy metal pollution restorative procedure |
| CN107671112A (en) * | 2017-09-11 | 2018-02-09 | 甘肃省地质矿产勘查开发局第三地质矿产勘查院 | Wild plant restoration method for heavy metal contaminated soil |
| CN108393338A (en) * | 2017-09-22 | 2018-08-14 | 河南科技大学 | A kind of method and application strengthened rye grass and repair metal strontium polluted soil |
| CN109317512A (en) * | 2018-09-29 | 2019-02-12 | 常州大学 | A kind of method that EDTA strengthens asparagus remediating lead-contaminated soil |
| CN110523774A (en) * | 2019-09-30 | 2019-12-03 | 武汉工程大学 | The method for discarding lead contamination in ground using the plant combined removal phosphorus ore of indigenous microorganism- |
| CN110523774B (en) * | 2019-09-30 | 2022-01-11 | 武汉工程大学 | Method for removing lead pollution in phosphorite waste land by utilizing indigenous microorganism-plant combination |
| CN113909282A (en) * | 2021-10-29 | 2022-01-11 | 南京大学 | Plant management method for heavy metal contaminated soil |
| CN115152542A (en) * | 2022-05-24 | 2022-10-11 | 四川农业大学 | Application method of brassinolide leaf surface blocking and controlling agent in Chinese cabbage planting |
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