CN103865545A - Method for concentrating soil heavy metals of sewage irrigation area by coalition of composite restoring agent and festuca arundinacea - Google Patents
Method for concentrating soil heavy metals of sewage irrigation area by coalition of composite restoring agent and festuca arundinacea Download PDFInfo
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- CN103865545A CN103865545A CN201410122693.1A CN201410122693A CN103865545A CN 103865545 A CN103865545 A CN 103865545A CN 201410122693 A CN201410122693 A CN 201410122693A CN 103865545 A CN103865545 A CN 103865545A
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Abstract
The invention discloses a method for concentrating soil heavy metals of a sewage irrigation area by coalition of a composite restoring agent and festuca arundinacea, wherein the composite restoring agent consists of a chelating agent nitrilotriacetic acid (NTA) and a surfactant sodium dodecyl sulfate, wherein the mol ratio of the chelating agent nitrilotriacetic acid to the surfactant sodium dodecyl sulfate is (5-15) mmol.kg<-1>:1mmol.kg<-1>. Experimental results show that a most remarkable effect of increasing the content of Cd in the festuca arundinacea is achieved due to the addition of the surfactant and the chelating agent, the concentration of the heavy metals can be promoted through co-addition of the surfactant and the chelating agent, and especially the effect is more remarkable when 5 mmol.kg<-1> of the NTA and 1 mmol.kg<-1> of the chelating agent or 10 mmol.kg<-1> of the NTA and 1 mmol.kg<-1> of the chelating agent are used for restoring sewage irrigation soil.
Description
Technical field
The invention belongs to environmental protection technical field, relate to the method for the dirty Irrigated Area Soils heavy metal of complex repairation agent associating Festuca Arundinacea enrichment.
Background technology
China's gross amount of water resources position is at the forefront in the world, but occupancy volume per person is considerably less, is one of 13 poor-water states, and China's water resources is also faced with various pollution problems in addition, and water resources lacks has become one of important factor of restriction China Economic development.Reasonably use sewage irrigation can alleviate the problem of water resources anxiety, can also saving water resource, alleviate the pollution to environment.Dirty filling refers to be carried out after harmless treatment city domestic sewage and trade effluent, directly or indirectly recharges for Sewage Irrigation in Farmland, gardens sewage irrigation and groundwater reservoir.China starts to build sewage irrigation engineering in nineteen fifty-seven after the founding of the state, lasts so far over half a century, and China has also passed through relevant dirt filling principle, policy and standard during this period.
The shortage of water resources has been alleviated in sewage irrigation to a certain extent, still fills with the expansion of scale along with dirt, more and more obvious on the impact of soil.Change physico-chemical property, Organic pollutants and the heavy metal contamination of soil.Sewage irrigation is one of important sources of heavy metal-polluted soil, has in 7 heavy metal higher than background value, and have the trend increasing gradually in the dirty Irrigated Area Soils of the cool phoenix in Beijing; The dirty Irrigated Area Soils in Baiyin city is subject to heavy metal ion severe contamination, need to be through many kinds of measures comprehensive regulation ability pollution remission situation.
In sewage, contain a large amount of heavy metal elements, in soil, accumulation can affect absorption and the utilization of plant to nutrient, also the metabolic balance that can upset plant carries out field experiment in Beijing suburb, result shows the dirty plant height, the leaf area index that have affected summer corn of filling with, and its output and dry-matter are obviously reduced.The pollution main manifestations of dirty irrigated area crop quality is that in agricultural-food, heavy metal content exceeds standard and the change of nutritive ingredient.There are some researches show, use each quality parameter of sewage irrigation mud field rice all to irrigate the obvious decline that has in mud field than clear water.Also have research to detect the agricultural-food such as the terraneous potato of soil, beet of sewage irrigation, result shows that wherein in Cd content and the soil solution, Cd content is linear dependence.
Agriculture environmental protection place is investigated the agricultural-food of more than 300 zone of pollution of 24 provinces and cities in the whole nation for 1997, and result shows that wheat, corn heavy metals exceeding standard rate are 15.5%, 14.0%, and heavy metal contamination is mainly take mercury, chromium, cadmium, arsenic etc. as main.Sewage irrigation also can make the protein content of wheat and paddy rice reduce, and along with the prolongation of sewage irrigation duration, the quality of crop can decline year by year.Dirty filling also can significantly be reduced Vitamin C content in vegetables, and the content of other nutritive ingredients is to have to increase to have to subtract.
Heavy metal in sewage is mainly enriched between the 0-20 cm of dirty Irrigated Area Soils top layer.Soil has reduced heavy metal to underground migration to the absorption of heavy metal.There are some researches show long-term dirty area of filling with, local underground water is not subject to the pollution of heavy metal, and in most cases, the heavy metal of dirt in pouring water can't significantly affect underground water generates.Also there are some researches show, the heavy metal in soil especially Cd can show with the increase of leaching time the trend of downward migration, thereby underground water is threatened.In a word, in dirty sewage of filling with, some pollutent is by adsorption by soil, and another part can move to soil lower floor, finally shallow ground water is affected.
Sewage irrigation causes soil and pollution of agricultural products on the one hand, makes pollutent in crop or the middle accumulation of winning, and then enters people by food chain, then causes the sickness rate of various chronic diseases to rise; Long-term dirt filling can cause Groundwater body pollution on the other hand, also can endanger human and livestock health after the daily drinking-water of people or livestock consumption; Also have long sewage irrigation, peasant's physical appearance of direct contact is also had to impact.Edible such agricultural-food for a long time as having indivedual areas, 19 areas such as the Shaoguan in the Ganzhou in Jiangxi and Dayu and Guangdong and the Qujiang River, Hunan, Shaanxi produce the situations of " cadmium rice ", if can work the mischief to the healthy of people.
Owing to directly irrigating with a large amount of raw sewerages, overproof water quality, the dirty area of filling with blindly expands again, causes the pollution of soil and crop and underground water, and sewage irrigation has been the one of the main reasons that causes China's Rural Water Environment to worsen.Sewage irrigation major part is that peasant is spontaneous, and their majority is in the situation that can not get clear water, and spontaneous introducing city life and industry waste water is as water source for irrigation, and this suburb nearby in northern China big and medium-sized cities is very general.
Traditional heavy metal pollution of soil recovery technique has casting landfill method, dilution method, drip-rinsing process, physical partition method and chemical method etc.Compared with traditional processing mode, the major advantage of phytoremediation is that cost is low, and treatment facility is simple, is applicable to large-scale application, is beneficial to the maintenance of soil ecosystem, little to environmental perturbation, and the feature such as possess aesthetic value.
In the biological restoration of heavy metal pollution of soil, application is phytoremediation technology early, more widely.Phytoremediation is exactly the heavy metal contaminants utilizing in plant absorption, accumulation and fixing soil, is transferred to root or over-ground part, then gathers in the crops at suitable growth phase, and after treatment, ashing is reclaimed heavy metal or carried out landfill.The result of study that Herba pteridis vittatae can a large amount of enrichment As of having reported for work first such as Chen Tongbin, analyzed the enriching quantity of this plant Different Organs to heavy metal simultaneously, the content of finding As in Herba pteridis vittatae Different Organs tissue is accessory pinna > petiole > root system, As easily upwards transportation and enrichment in this plant materials is described, demonstrating Herba pteridis vittatae has extremely strong patience and unique accumulation ability to As.Sun etc. study discovery, be grown in containing Cd content in the Sedum alfredii Hance plants stems in Cd slag soil and leaf apparently higher than root, the Sedum alfredii Hance plant root Cd content that is grown in non-Cd mining area is apparently higher than the content in cauline leaf, thereby confirms that Sedum alfredii Hance also has stronger inrichment to Cd.Tian Shengni etc. are by being preced with relatively thinking of grass with goose, and Caulis Miscanthis floriduli is that Cu, Pb, Zn Single Pollution or combined pollution are all had to good repairing effect.Ye Chun and studied reparation and the activating mechanism of alfalfa to Pb contaminated soil, finds out from X-ray micro-zone analysis result, intercellular substance Pb content is the highest, and cell walls and vacuole take second place, minimum in kytoplasm; Pb mainly exists with the form of indissoluble in alfalfa body, and alfalfa is relevant with the formation of plant complexing element to the tolerance of Pb.Because biomass of alfalfa is high, so it is the desirable rehabilitation plant of one that P in soil b pollutes that alfalfa can be used as.Secretions from plant roots can change rhizosphere soil environment, can make Cr, the Hg of multivalence state, valence state and the form of As change, and affects its toxic effect.The root hair of plant can directly increase root table from soil exchange adsorption heavy metal to be fixed.But the growth cycle of the super enriching plant for restoration of soil polluted by heavy metal having is grown, biomass is relatively little, and low for the bioaccumulation efficiency of the low heavy metal of some biological effectiveness, this is restricted single use phytoremediation.
In reality, pollutant in soil is of a great variety, combined pollution is general, and pollution level and difference in thickness are large, and epidermic soil type is many simultaneously, the area differentiation of its composition, character, condition is obvious, and after repairing, the space planning of soil recycling formula requires different.Therefore, individual event recovery technique is toward being difficult to reach reparation target, and exploitation complex repairation pattern just becomes the main direction of studying that soil pollution is repaired.
The main Types that starts now the complex repairation technology that drops into application has the reparation of plant/microbial association, animal/plant combined reparation and chemistry/materialization-Co-bioremediation.The mechanism of plant/microbial association reparation is that higher plant and soil microorganisms often exist synergy in process of growth, on the one hand plant can provide the soil microorganisms required carbon source of growing, simultaneously again through base of leaf to root delivering oxygen, be formed with the microenvironment that is beneficial to oxidation, promote the Decomposition of aerobic microbiological to pollutent.Chemistry/materialization-Co-bioremediation can be brought into play the advantage of short, instant effect of the cycle that chemistry or physics repairs, and in conjunction with nondestructive biological restoration feature, development is contaminated soil restorative procedure the most with potential applications based on chemical-biological recovery technique.As utilize the coordination stripping of organic complexing agent, and increase heavy metal concentration in the soil solution, improve the assimilated efficiency of plant, thereby realize the reparation of strengthening Enhanced phytoextraction.Therefore, improve the method that plant absorbs enriching heavy metal, except using and continuing to find accumulation ability is strong and biomass is large super enriching plant, we can add artificial or natural sequestrant and other enhancements and improve the ability of plant absorption enriching heavy metal in phytoremediation process.
Sequestrant inducing plant extracts remediating heavy metal, and it can increase the solubleness of heavy metal in soil on the one hand, can improve on the other hand heavy metal rhizosphere diffusibility, can also promote heavy metal to transport to overground part from root system.Pealosa etc. have studied the factor that several promotion lupine repairing heavy metal in soil pollute, and found that, sequestrant NTA can promote metal ion transport, impel the contained concentration of metal ions of lupine to raise, and especially As, Cd and the increase of Pb concentration are more obvious.Qian Meng etc. plant elsholtzia splendens on Cu, Zn, Pb, Cd combined contamination soil, use 5 mmolkg
-1after EDTA, the concentration of Cu, Zn, Pb, Cd improves 27.4,3.2,17.6 and 89.5 times than contrast respectively.The researchs such as Stanhope show, along with the increase of EDTA concentration, the ratio that in soil, the content of Cu, Zn, Cd, Pb, Ni soluble state heavy metal accounts for total heavy metal content is linear raising.In chelation evoked phytoremediation process, the duration of service of sequestrant is very important, generally in 1-2 week, to add before plant results, now growth and development of plants maturation, less on increment impact after processing in the short period of time, the total metals of plant extract can significantly increase.
By warmhouse booth potted plant experiment, research sequestrant and tensio-active agent is independent or the effect of the auxiliary golden good fortune mushroom restoration of soil polluted by heavy metal of Combined Processing.Result shows, the EDTA of high density makes the biomass of Jin Fugu reduce than contrast, the concentration of sporophore Pb, Cu and Cd respectively than contrast improve 15-88 doubly, 0.8-3.3 doubly and 0.5-0.6 doubly.But the toxicity of EDTA and leaching risk make it be subject to application limitations.Tensio-active agent also may increase the absorption of heavy metal relevant with the saturating property of its increase cytolemma outside the Pass promoting that thereby the plant absorption mechanism of action of heavy metal and the desorb of its promotion heavy metal in soil, solubilising raising bioavailability of heavy metals have.These researchs are take edible crop as research object mostly, promote plant accumulation heavy metal by adding sequestrant, but some sequestrants have reduced the biomass of plant in raising plant materials in heavy metal content, and sequestrant itself is not easy degraded, this may cause certain hidden danger to the seepage migration of the plant treatment in later stage and sequestrant so.
Most reports and research at present all shows that China's sewage irrigation soil has heavy metal contamination in various degree, this also has the mankind's harm to environment is apparent, and at present the achievement in research in laboratory also temporarily can not be applied to land for growing field crops, find feasible, to be applicable to repair dirty method or technology of filling with native heavy metal very necessary.Heavy metal pollution of soil has the features such as the poly-property of complicacy, non-reversibility and table, single improvement method is difficult to be removed clean, the recovery technique that two or more recovery technique combines, can better bring into play advantage separately, is more beneficial to the reparation of heavy metal pollution of soil.Combination recovery technique is the burning hoter recovery technique of Recent study.
The collaborative phytoremediation heavy metal contamination of sequestrant in the past using, as EDTA, the mixture that sequestrant itself and it and heavy metal form has very strong bio-toxicity and solvability, and in soil, be difficult to be degraded by light, biology or other chemical substance, be easy to pollute deep soil and underground water, and quantity and the species diversity of EDTA to soil microorganisms and microfauna can exert an influence, and then may exert an influence to soil ecosystem.Therefore, the utilisation technology of novel renovation agent is especially paid close attention to.
Repair dirt with lawn plant and fill with soil, add degradable sequestrant, respectively again using tensio-active agent, microorganism, growth hormone as auxiliary, make lawn plant accumulate more heavy metal, this not only can be avoided heavy metal to enter food chain harm humans, also turf volume can be applied to urban afforestation, the stronger reproducibility of lawn plant is also suitable for repairing in addition simultaneously.Administer heavy metal pollution of soil, phytoremediation, because cheapness, environmental friendliness etc. are widely used, also has many research, activates heavy metal, to promote absorption and transport and the accumulation of plant by adding sequestrant.Tensio-active agent, because it has solubilising, the characteristic such as incremental, can cement out heavy metal and be present in the soil solution with the form of complexing, inner complex from soil, increases bioavailability, promotes absorption and the extraction of plant to heavy metal.Tensio-active agent is because solubilizing effect is good, the cycle is short, currently become heavy metal and one of recovery technique of tool potentiality is repaired in organic contamination.In single use tensio-active agent or sequestrant rehabilitating soil, the effect of heavy metal is limited, some scholar begins one's study and utilizes heavy metal contamination in tensio-active agent and the compound removal of sequestrant and isolating environment, there are some researches show that sequestrant and tensio-active agent are compound rear better than independent use tensio-active agent to the removal effect of heavy metal.In recent years, also having research that sequestrant is combined with tensio-active agent is applied in heavy metal reparation.
Summary of the invention
The object of the present invention is to provide the method for the dirty Irrigated Area Soils heavy metal of a kind of complex repairation agent associating Festuca Arundinacea enrichment.The present invention, on the basis of optimization experiment, attempts the strengthening Festuca Arundinacea heavy metal of drawing accumulation fund by adding NTA and sodium laurylsulfonate, to reach good repairing effect.For realizing this object, the invention provides following technical scheme:
A kind of complex repairation agent, is characterized in that it is made up of sequestrant nitrilotriacetic acid(NTA) and tensio-active agent sodium laurylsulfonate, and wherein the mol ratio of sequestrant nitrilotriacetic acid(NTA) and tensio-active agent sodium laurylsulfonate is 5-15mmolkg
-1nTA:1 mmolkg
-1sodium laurylsulfonate.
The method that the present invention further discloses the dirty Irrigated Area Soils heavy metal of complex repairation agent associating Festuca Arundinacea enrichment, is characterized in that being undertaken by following step:
(1) processing of material:
Select the more common perennial Festuca Arundinacea of northern China (
festuca arundinaceal
.) be experiment material;
Sequestrant is selected nitrilotriacetic acid(NTA) (NTA), and tensio-active agent is selected sodium laurylsulfonate;
After the soil of collection is removed to grass roots, stone, divide on transparent plastic cloth, be positioned over ventilation, after natural air drying 2 ~ 3 d, cross 2 mm sieves for subsequent use; The basic physical and chemical of soil: soil organic matter content 3.62%, total nitrogen content 0.19%, full phosphorus amount 5.4 gkg
-1, full potassium amount 787.3 mgkg
-1, pH7.28, soil moisture content 4.13%, specific conductivity 0.44 mscm
-1.In soil, the content of Cd, Cu and Zn is respectively 7.13,146.31 and 795.56 mgkg
-1, be respectively 23.8,1.5 and 3.2 times of soil environment quality secondary standard (GB156182-1995);
(2) experimental technique:
In being the culture dish of 9 cm, diameter add 90g for examination soil respectively, each culture dish sowing 0.5g Festuca Arundinacea, sooner or later water on time, plant-growth 40d, respectively the NTA of respective concentration or sodium laurylsulfonate are pressed to required dosage according to experimental design, the disposable stromal surface that puts on respective handling group; After processing 10d, gather in the crops lawn plant, be 50 days experimental period, and lawn plant is cultivated and carries out in laboratory, and often reversing of position is consistent to guarantee illumination, room temp 14-28 ℃ between incubation period, relative humidity 33 ~ 62%; Wherein the NTA of respective concentration or sodium laurylsulfonate refer to wherein one below:
5-15?mmol·kg
-1NTA；
5 mmolkg
-1nTA+1 mmolkg
-1sodium laurylsulfonate;
10 mmolkg
-1nTA+1 mmolkg
-1sodium laurylsulfonate;
15 mmolkg
-1nTA+1 mmolkg
-1sodium laurylsulfonate;
(3) mensuration of biomass: plant and gather in the crops lawn plant after 50 days, be divided into ground and underground part, after deionized water rinses repeatedly, put it in baking oven, under 80 ℃ of conditions, dry to constant weight, survey its dry weight, finally measure heavy metal content in Digestive system with TAS-990 atomic absorption spectrophotometer.
The application of the method that the present invention further discloses the dirty Irrigated Area Soils heavy metal of complex repairation agent associating Festuca Arundinacea enrichment aspect the dirty Irrigated Area Soils heavy metal of enrichment.Described Festuca Arundinacea enriching heavy metal refers to the content of Cd in enrichment Festuca Arundinacea.
The present invention further discloses complex repairation agent and has referred to 5 mmolkg
-1nTA:1 mmolkg
-1sodium laurylsulfonate or 10 mmolkg
-1nTA:1 mmolkg
-1sodium laurylsulfonate.
The conclusion that the present invention reaches by test is as follows:
The raising effect of the content of the interpolation of promoting agent (tensio-active agent) and sequestrant to Cd in Festuca Arundinacea is the most remarkable, and common interpolation can promote the enrichment of heavy metal, particularly uses 5 mmolkg
-1nTA+1 mmolkg
-1sodium laurylsulfonate or 10 mmolkg
-1nTA+1 mmolkg
-1dirt is filled with to soil, and to carry out repairing effect more remarkable.
The more detailed preparation method of the present invention is as follows:
1 materials and methods
1.1 experiment material
Select the more common perennial Festuca Arundinacea of northern China (
festuca arundinaceal
.) be experiment material.
Sequestrant is selected nitrilotriacetic acid(NTA) (NTA), and molecular formula is N (CH
2cOOH)
3, be purchased from Chemical Reagent Co., Ltd., Sinopharm Group, be analytical pure.
Tensio-active agent is selected sodium laurylsulfonate, and molecular formula is C
12h
25naO
3s, is purchased from Beijing chemical reagents corporation, is analytical pure.
Take from dirty irrigated area, Xiqing District, Tianjin for examination soil, by dividing on transparent plastic cloth after the soil removal grass roots of collection, stone, be positioned over ventilation, after natural air drying 2 ~ 3 d, cross 2 mm sieves for subsequent use.Soil organic matter content 3.62%, total nitrogen content 0.19%, full phosphorus amount 5.4 gkg
-1, full potassium amount 787.3 mgkg
-1, pH7.28, soil moisture content 4.13%, specific conductivity 0.44 mscm
-1.In soil, the content of Cd, Cu and Zn is respectively 7.13,146.31 and 795.56 mgkg
-1, be respectively 23.8,1.5 and 3.2 times of soil environment quality secondary standard (GB156182-1995).
1.2 experimental technique
Experimental design: not adding NTA and sodium laurylsulfonate is contrast (I); 5 mmolkg
-1nTA(II); 10 mmolkg
-1nTA(III); 15 mmolkg
-1nTA(IV); 5 mmolkg
-1nTA+1 mmolkg
-1sodium laurylsulfonate (V); 10 mmolkg
-1nTA+1 mmolkg
-1sodium laurylsulfonate (VI); 15 mmolkg
-1nTA+1 mmolkg
-1sodium laurylsulfonate (VII).Each processing repeats for 3 times.
Be in the culture dish of 9 cm, to add 90g for examination soil to diameter, each culture dish is sowed 0.5 g Festuca Arundinacea, sooner or later waters on time.Plant-growth 40d, is dissolved in distilled water by the NTA of respective concentration or sodium laurylsulfonate by required dosage respectively according to experimental design, the disposable stromal surface that puts on respective handling group; The distilled water pouring of equivalent for contrast.After processing 10d, gather in the crops lawn plant, be 50 days experimental period.Lawn plant is cultivated and carries out in laboratory, and often reversing of position is consistent to guarantee illumination, 14 ~ 28 ℃ of room temps between incubation period, relative humidity 33 ~ 62%.
1.3 index determining
The mensuration of biomass: plant the lawn plant of receiving after 50 days, be divided on the ground and underground part, after deionized water rinses repeatedly, put it in baking oven, dry to constant weight under 80 ℃ of conditions, survey its dry weight.
The mensuration of heavy metal content: weigh plant sample 0.1 g, with nitric acid: perchloric acid: after sulfuric acid (8:1:1) is cleared up, gained solution is settled to 25 mL with distilled water, finally measures heavy metal (Cd, Cu, Zn) content in Digestive system with TAS-990 atomic absorption spectrophotometer.
1.4 data analysis processing
Adopt SPSS17.0 software to carry out statistical study to data.
2 development results analyses
The impact on Festuca Arundinacea biomass of 2.1 NTA and sodium laurylsulfonate
as shown in Table 1, add each treatment group ground biomass that NTA and NTA and sodium laurylsulfonate combine interpolation and the difference that contrasts that there are no significant (
p> 0.05).Add 10 mmolkg
-1it is 1.12 times of contrast that the ground biomass of NTA treatment group reaches maximum value, and is significantly higher than 15 mmolkg
-1nTA group, 5 mmolkg
-1nTA+1 mmolkg
-1sodium laurylsulfonate group, 15 mmolkg
-1nTA+1 mmolkg
-1sodium laurylsulfonate group.Combine interpolation NTA and sodium laurylsulfonate treatment group underground biomass and all increase than contrast, particularly 5 mmolkg
-1nTA+1 mmolkg
-1sodium laurylsulfonate group and 10 mmolkg
-1nTA+1 mmolkg
-1sodium laurylsulfonate group is significantly higher than control group.
The impact of table 1 different treatment on Festuca Arundinacea biomass
With the different letter representation significant differences of column data (
p<0.05)
The impact on Festuca Arundinacea overground part enriching heavy metal of 2.2 NTA and sodium laurylsulfonate
As can be seen from Table 2, NTA combines the raising that use is conducive to Festuca Arundinacea overground part Cd, Cu, Zn content with sodium laurylsulfonate.15 mmolkg
-1nTA+1 mmolkg
-1sodium laurylsulfonate group Festuca Arundinacea overground part Cd, Cu content are significantly higher than 15 mmolkg
-1nTA treatment group, has improved respectively 40.5%, 36%.Add and compare with concentration NTA group but NTA and sodium laurylsulfonate are common, the content of Zn improves not obvious.Each processing Festuca Arundinacea overground part Accumulation of heavy metals amount is relevant with overground part heavy metal concentration and biomass, as can be seen from the table, and 10 mmolkg
-1nTA+1 mmolkg
-1sodium laurylsulfonate group Festuca Arundinacea overground part Cd, Cu total amount reach maximum value, and be significantly higher than contrast (
p< 0.05).No matter be to add separately the common content and the accumulation volume that have all improved significantly Festuca Arundinacea overground part Zn of adding of NTA or NTA and sodium laurylsulfonate.
Table 2 different treatment absorbs the impact of heavy metal on Festuca Arundinacea overground part
With the different letter representation significant differences of column data (
p<0.05)
The impact on Festuca Arundinacea root enriching heavy metal of 2.3 NTA and sodium laurylsulfonate
Table 3 is the impacts on Festuca Arundinacea root absorption heavy metal about different treatment.NTA and sodium laurylsulfonate all can make the content of Festuca Arundinacea root Cd and accumulation total amount be significantly higher than other treatment group after combining interpolation.15 mmolkg
-1nTA+1 mmolkg
-1sodium laurylsulfonate group root Cu content is significantly higher than other groups (10 mmolkg
-1except NTA group).In control group, the content of Festuca Arundinacea root Zn is significantly less than other each treatment group, but NTA and the content difference that adds Festuca Arundinacea root Zn in the treatment group of sodium laurylsulfonate with concentration NTA not significantly (
p> 0.05).Festuca Arundinacea root Cu, the Zn gross accumulation amount of each processing are all significantly higher than control group.
The impact of table 3 different treatment on Festuca Arundinacea root absorption heavy metal
With the different letter representation significant differences of column data (
p<0.05)
3 development conclusions
The raising effect of the content of the interpolation of promoting agent and sequestrant to Cd in Festuca Arundinacea is the most remarkable, and the raising of Cu, Zn content in Festuca Arundinacea is had to certain limitation.And Festuca Arundinacea is also to need sequestrant and tensio-active agent just can reach peaked under certain condition to the accumulation volume of heavy metal.All in all, both common interpolations can promote the enrichment of heavy metal, consider biomass, heavy metal accumulation and cost situation, can use 5 mmolkg
-1nTA+1 mmolkg
-1sodium laurylsulfonate or 10 mmolkg
-1nTA+1 mmolkg
-1dirt is filled with to soil repairs.
Embodiment:
Below in conjunction with specific embodiment, the present invention will be further described, and following each embodiment is not only limitation of the present invention for the present invention is described.Wherein chemical reagent used all has commercially available.
Embodiment 1
A method for the dirty Irrigated Area Soils heavy metal of complex repairation agent associating Festuca Arundinacea enrichment, is characterized in that being undertaken by following step:
(1) processing of material:
Select the more common perennial Festuca Arundinacea of northern China (
festuca arundinaceal
.) be experiment material;
Sequestrant is selected nitrilotriacetic acid(NTA) (NTA), and tensio-active agent is selected sodium laurylsulfonate;
After the soil of collection is removed to grass roots, stone, divide on transparent plastic cloth, be positioned over ventilation, after natural air drying 2 ~ 3 d, cross 2 mm sieves for subsequent use; The basic physical and chemical of soil: soil organic matter content 3.62%, total nitrogen content 0.19%, full phosphorus amount 5.4 gkg
-1, full potassium amount 787.3 mgkg
-1, pH7.28, soil moisture content 4.13%, specific conductivity 0.44 mscm
-1.In soil, the content of Cd, Cu and Zn is respectively 7.13,146.31 and 795.56 mgkg
-1, be respectively 23.8,1.5 and 3.2 times of soil environment quality secondary standard (GB156182-1995);
(2) experimental technique:
In being the culture dish of 9 cm, diameter add 90g for examination soil respectively, each culture dish sowing 0.5g Festuca Arundinacea, sooner or later water on time, plant-growth 40d, respectively the NTA of respective concentration or sodium laurylsulfonate are pressed to required dosage according to experimental design, the disposable stromal surface that puts on respective handling group; After processing 10d, gather in the crops lawn plant, be 50 days experimental period, and lawn plant is cultivated and carries out in laboratory, and often reversing of position is consistent to guarantee illumination, 14 ℃ of room temps between incubation period, relative humidity 33%; Wherein respective concentration: 5 mmolkg
-1nTA+1 mmolkg
-1sodium laurylsulfonate;
(3) mensuration of biomass: plant and gather in the crops lawn plant after 50 days, be divided into ground and underground part, after deionized water rinses repeatedly, put it in baking oven, under 80 ℃ of conditions, dry to constant weight, survey its dry weight, finally measure heavy metal Cd, Cu and Zn content in Digestive system with TAS-990 atomic absorption spectrophotometer.
Embodiment 2
(1) processing of material:
Select the more common perennial Festuca Arundinacea of northern China (
festuca arundinaceal
.) be experiment material;
Sequestrant is selected nitrilotriacetic acid(NTA) (NTA), and tensio-active agent is selected sodium laurylsulfonate;
After the soil of collection is removed to grass roots, stone, divide on transparent plastic cloth, be positioned over ventilation, after natural air drying 2 ~ 3 d, cross 2 mm sieves for subsequent use; The basic physical and chemical of soil: soil organic matter content 3.62%, total nitrogen content 0.19%, full phosphorus amount 5.4 gkg
-1, full potassium amount 787.3 mgkg
-1, pH7.28, soil moisture content 4.13%, specific conductivity 0.44 mscm
-1.In soil, the content of Cd, Cu and Zn is respectively 7.13,146.31 and 795.56 mgkg
-1, be respectively 23.8,1.5 and 3.2 times of soil environment quality secondary standard (GB156182-1995);
(2) experimental technique:
In being the culture dish of 9 cm, diameter add 90g for examination soil respectively, each culture dish sowing 0.5g Festuca Arundinacea, sooner or later water on time, plant-growth 40d, respectively the NTA of respective concentration or sodium laurylsulfonate are pressed to required dosage according to experimental design, the disposable stromal surface that puts on respective handling group; After processing 10d, gather in the crops lawn plant, be 50 days experimental period, and lawn plant is cultivated and carries out in laboratory, and often reversing of position is consistent to guarantee illumination, 28 ℃ of room temps between incubation period, relative humidity 50%; Wherein respective concentration: 10 mmolkg
-1nTA+1 mmolkg
-1sodium laurylsulfonate;
(3) mensuration of biomass: plant and gather in the crops lawn plant after 50 days, be divided into ground and underground part, after deionized water rinses repeatedly, put it in baking oven, under 80 ℃ of conditions, dry to constant weight, survey its dry weight, finally measure heavy metal Cd, Cu and Zn content in Digestive system with TAS-990 atomic absorption spectrophotometer.
Claims (6)
1. a complex repairation agent, is characterized in that it is made up of sequestrant nitrilotriacetic acid(NTA) and tensio-active agent sodium laurylsulfonate, and wherein the mol ratio of sequestrant nitrilotriacetic acid(NTA) and tensio-active agent sodium laurylsulfonate is 5-15mmolkg
-1nTA:1 mmolkg
-1sodium laurylsulfonate.
2. a method that adopts the dirty Irrigated Area Soils heavy metal of complex repairation agent associating Festuca Arundinacea enrichment claimed in claim 1, is characterized in that being undertaken by following step:
(1) processing of material:
Select the more common perennial Festuca Arundinacea of northern China (
festuca arundinaceal
.) be experiment material;
Sequestrant is selected nitrilotriacetic acid(NTA) (NTA), and tensio-active agent is selected sodium laurylsulfonate;
After the soil of collection is removed to grass roots, stone, divide on transparent plastic cloth, be positioned over ventilation, after natural air drying 2-3 d, cross 2 mm sieves for subsequent use;
(2) experimental technique:
In being the culture dish of 9 cm, diameter add 90g for examination soil respectively, each culture dish sowing 0.5g Festuca Arundinacea, sooner or later water on time, plant-growth 40d, respectively the NTA of respective concentration or sodium laurylsulfonate are pressed to required dosage according to experimental design, the disposable stromal surface that puts on respective handling group; After processing 10d, gather in the crops lawn plant, be 50 days experimental period, and lawn plant is cultivated and carries out in laboratory, and often reversing of position is consistent to guarantee illumination, room temp 14-28 ℃ between incubation period, relative humidity 33 ~ 62%; Wherein the NTA of respective concentration or sodium laurylsulfonate refer to wherein one below:
5-15?mmol·kg
-1NTA；
5 mmolkg
-1nTA+1 mmolkg
-1sodium laurylsulfonate;
10 mmolkg
-1nTA+1 mmolkg
-1sodium laurylsulfonate;
15 mmolkg
-1nTA+1 mmolkg
-1sodium laurylsulfonate;
(3) mensuration of biomass: plant and gather in the crops lawn plant after 50 days, be divided into ground and underground part, after deionized water rinses repeatedly, put it in baking oven, under 80 ℃ of conditions, dry to constant weight, survey its dry weight, finally measure heavy metal content in Digestive system with TAS-990 atomic absorption spectrophotometer.
3. the method for the dirty Irrigated Area Soils heavy metal of complex repairation agent associating Festuca Arundinacea enrichment claimed in claim 2, heavy metal wherein refers to Cd, Cu, Zn content.
4. the application of the method for the dirty Irrigated Area Soils heavy metal of complex repairation agent associating Festuca Arundinacea enrichment aspect the dirty Irrigated Area Soils heavy metal of enrichment described in claim 2.
5. application claimed in claim 4, wherein said Festuca Arundinacea enriching heavy metal refers to the content of Cd in enrichment Festuca Arundinacea.
6. application claimed in claim 4, wherein said complex repairation agent refers to 5 mmolkg
-1nTA:1 mmolkg
-1sodium laurylsulfonate or 10 mmolkg
-1nTA:1 mmolkg
-1sodium laurylsulfonate.
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| CN112574751A (en) * | 2019-09-29 | 2021-03-30 | 中国石油化工股份有限公司 | Ferric-iron-containing heavy metal leaching composition, application thereof and method for repairing heavy metal soil |
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