CN106337030A - Microbial product for treatment of heavy metal polluted saline-alkaline land and manufacture method thereof - Google Patents
Microbial product for treatment of heavy metal polluted saline-alkaline land and manufacture method thereof Download PDFInfo
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- CN106337030A CN106337030A CN201610713565.3A CN201610713565A CN106337030A CN 106337030 A CN106337030 A CN 106337030A CN 201610713565 A CN201610713565 A CN 201610713565A CN 106337030 A CN106337030 A CN 106337030A
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- heavy metal
- plant
- salt
- base material
- microbial product
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The invention discloses a microbial product for treatment of heavy metal polluted saline-alkaline land and a manufacture method thereof. The product comprises a nutrient medium and a target bacterial strain. The nutrient medium is a powdery nutrient medium and comprises a protein plant, an energy plant and a fiber plant. The target bacterial strain is specifically bacillus thuringiensis, Halorubrum alkaliphilum, Natronococcus and white rot fungi. The manufacture method comprises the following steps: preparing the nutrient medium, culturing the target bacterial strain, preparing a microbial product, and preserving the microbial product. The microbial product for treatment of heavy metal polluted saline-alkaline land is especially suitable for heavy metal polluted saline-alkaline land. By the use of the microbial product, harm of heavy metal pollution can be reduced, fertility of saline-alkaline land is raised, pH value of saline-alkaline land is improved, and normal plantation of crops is not affected during treatment.
Description
Technical field
The present invention relates to heavy metal prevention and control field, more particularly, to a kind of microbial product administering heavy metal pollution salt-soda soil
And its manufacture method.
Background technology
Salt-soda soil is a species of salt aggregation, refers to that contained salinity inside soil has influence on the normal life of crop
Long, according to UNESCO and food and agricultural organization's incomplete statistics, the area in whole world salt-soda soil is 9.5438 hundred million hectares,
Wherein China is 99,130,000 hectares.China's alkaline earth and the formation of alkali-affected soil, most of accumulative relevant with Carbonates In Soil,
Thus basicity is generally higher, serious salt affected soil regional plant can hardly be survived.
Heavy Metal Pollution Control in salt-soda soil has two big difficult points: one be pollutant in soil poor mobility, when being detained
Between long, be unable to natural degradation, dilution, flowing, can prolongation over time be enriched with all the more in salt-soda soil, harm is planted in salt-soda soil
The plant planted；Two is that plant survival rate is very low, and improvement is extremely slow naturally because salt content is high and ph is in alkalescence.People couple in recent years
In the pollution problem pay attention to day by day of soil, for mitigating the pollution to soil for the heavy metal, people have done many researchs, have developed each
Plant different treatment technologies, one is activation, mainly utilize different means to increase the activeness of heavy metal in soil, finally
Heavy metal is excluded from soil；Two is immobilization role, is changed into the heavy metal in soil using different means
More stable component is so as to be difficult to be reached the purpose of cover crop by crop utilization.
Current restorative procedure mainly includes electric repair method, chemical leaching method and plant restoration method etc., this
The advantage of class method is the heavy metal that can remove in soil, but there is power consumption height in application, is difficult to realize in-situ immobilization
The shortcomings of.For activation method, although fixing meanss do not enable the removal of heavy metal, easy and simple to handle, cost
Cheap, it is easy to accomplish in-situ immobilization, traditional process for fixation mainly adds different types of chemical substance in soil,
Adsorb, chelate and precipitation to realize the immobilization of heavy metal with heavy metal.
At home in the Patents of application, it is not specifically designed for the correlation that salinized soil carries out microbial treatment
Technical data.In near field, patent " a kind of repairing method of microorganism of heavy-metal contaminated soil " (application number:
201110122739.6, publication date: 2011-11-16) disclose one kind using Spherical red antibacterial preparation cooperation waterflooding improvement soil
Method, its operation principle predominantly utilizes Adsorption of Heavy Metals after Spherical red antibacterial amount reproduction under rich water anaerobic environment, then
Permeate the ground the heavy metal concentration reducing in soil via water, but its soil in governance process cannot be used for planting operation, warp
Ji high cost, on the other hand, is not suitable for the region of salinization of soil yet, and heavy metal flows into subsoil water and will also result in secondary dirt
Dye；Patent " heavy-metal contaminated soil microorganism renovation agent and its application " (application number: 201410682558.2, publication date:
2015-04-22) disclose a kind of employing bacillus thuringiensiss and candida tropicalises as the heavy metal pollution of active ingredient
Soil remediation agent, the bioremediation agents of this invention can adsorb a certain proportion of heavy metal, and when can retain one section in soil
Between, but candida tropicalises belong to anaerobism mushroom, in the soil that ventilation is dried cannot long-term surviving, on the other hand, effective strain
Without the domestication of targetedly heavy metal, the tolerance to corresponding heavy metal is poor, and the rich effect of collection is also bad, and due to every kind of microorganism
Have its limitation, therefore its to administer the effect of soil neither long-acting, nor comprehensive.
Content of the invention
For drawbacks described above present in prior art, the present invention is intended to provide a kind of be applied to the high alkaline soil of salinity
Earth, has degraded heavy metal and promoting growth of plants effect, permanently effective, active ingredient more targetedly tolerates target weight simultaneously
The microbial product in the improvement heavy metal pollution salt-soda soil of metal.
To achieve these goals, the present invention employs the following technical solutions: a kind of administer heavy metal pollution salt-soda soil micro-
Biological product, including Nutrient medium and objective microbe, wherein: described Nutrient medium is Powdered nutritional base, compare 5 including with weight:
The protide low cost plant powder of the ratio mechanical mixture of 2:3, energy class low cost plant base material powder, fiber-like low cost
Plant powder；Described objective microbe is aerobe, including bacillus thuringiensiss, the red bacterium of basophilic salt, thermophilic saline and alkaline coccus
And white rot fungi.
The microbial product in above-mentioned improvement heavy metal pollution salt-soda soil, wherein: protide low cost plant base material is preferably
Semen Glyciness, energy class low cost plant base material is preferably Semen Maydiss or Semen arachidis hypogaeae, and fiber-like low cost plant base material is preferably Testa oryzae or plant
Thing straw stalk.
The manufacture method of the above-mentioned microbial product in improvement heavy metal pollution salt-soda soil, its step includes producing nutrition
Base, training objective bacterial strain, produce microbial product, conserving microorganism product, wherein:
1) described Nutrient medium forming steps are:
1. prepare base material: from protide low cost plant base material, energy class low cost plant base material, fiber-like low cost plant
The each at least one of base material；
2. adopt the oils and fatss that mechanical expression and/or Chemical Leaching extract in base material, the plant residue after deoiling is dried stand-by；
3. using mechanical system, plant residue is clayed into power shape；
4. carry out using the powdery plant residue that 150 DEG C -200 DEG C of temperature previous step obtains that high temperature is degerming and baking removes
Water, you can obtain described Powdered nutritional base.
2) described aimed strain is specially the resistant strain polluting corresponding heavy metal, and its forming steps is:
1. contaminated salt-soda soil is analyzed detecting, specifies overproof heavy metal species in salt-soda soil；
2. the heavy metal species 1. being detected according to step, from entering to bacterial strain of corresponding waste and old heavy metal or heavy metal slag
Row domestication, then using standard culture procedures, purification and separation are carried out to the bacterial strain after domestication；Described purification process choosing is preferably flat
Plate method of scoring, at least divides 4 areas, and purification cycle is 3-4 week, at least purification 2 times；By all kinds of bacterial strains obtaining after purification respectively
Separate from each self-corresponding culture medium, that is, obtain described aimed strain.
The manufacture method of the microbial product in above-mentioned improvement heavy metal pollution salt-soda soil it is characterised in that: described employing machine
The method of the oils and fatss in tool squeezing and/or Chemical Leaching extraction base material, preferably first extracts high-quality oils and fatss using mechanical expression method
Adopt Chemical Leaching method second extraction afterwards again.
Compared with the prior art, the present invention, due to employing such scheme, has the advantage that the Nutrient medium of selection at least
Including cellulose, protein and saccharide nutrient, for preferred aerobic-type chemoheterotrophic bacteria (bacillus thuringiensiss, basophilic salt
Red bacterium, thermophilic saline and alkaline coccus) and white rot fungi provide the excellent environment of growth and breeding, can make effective strain be dried ventilation salt
Fast-propagation in alkali ground；Thermophilic saline and alkaline coccus and basophilic salt red bacterium suitable growth ph value are 8-10, and salt-soda soil are typically being dried just
Often ph value quite, can muchly be survived, simultaneously because it belongs to aerobe, in the salt-soda soil after turning over plough in salt-soda soil is dried
Regulation effect will be made more efficient due to aerobic environment quick death；After specific domestication, effective strain can be more targetedly
Tolerance need to administer the heavy metal species in salt-soda soil, and can improve enrichment makes antibacterial itself more muchly survive simultaneously；Due to white
Rotten funguses can quickly be decomposed to crop extremely bad rhizome, can increase salt-soda soil fertilizer degree, simultaneously while reducing and consume
Its secretions can by way of extracellular precipitation curing heavy metal, reduce its harm to plant, and make saline and alkaline soil be difficult plate
Knot, simultaneously its in decomposition of cellulose Nutrient medium or crop extremely bad rhizome when can produce acidic materials, can improved soil acid-base value,
It is allowed to gradually go salinization of soil；, only with the residue substantially useless to the mankind, Financial cost is low for Nutrient medium.
Specific embodiment
Embodiment 1:
A kind of microbial product administering heavy metal pollution salt-soda soil, including Nutrient medium and objective microbe, wherein: described nutrition
Base is Powdered nutritional base, including the Semen Glyciness powder of the ratio mechanical mixture than 5:2:3, Semen Maydiss or Semen arachidis hypogaeae powder, rice with weight
Bran or plant straw powder；Described objective microbe is aerobe, including bacillus thuringiensiss, the red bacterium of basophilic salt, thermophilic
Saline and alkaline coccus and white rot fungi.
The manufacture method of mentioned microorganism product, its step includes producing Nutrient medium, training objective bacterial strain, produces microorganism
Product, conserving microorganism product, wherein:
1) described Nutrient medium forming steps are:
1. prepare base material: from Semen Glyciness, Semen Maydiss or Semen arachidis hypogaeae, Testa oryzae or plant straw as base material；
2. first extract, using using mechanical expression method, the oils and fatss adopting again after high-quality oils and fatss in Chemical Leaching method second extraction base material,
Plant residue after deoiling is dried stand-by；
3. using mechanical system, plant residue is clayed into power shape；
4. carry out using the powdery plant residue that 150 DEG C -200 DEG C of temperature previous step obtains that high temperature is degerming and baking removes
Water, you can obtain described Powdered nutritional base.
2) described aimed strain is specially the resistant strain polluting corresponding heavy metal, and its forming steps is:
1. contaminated salt-soda soil is analyzed detecting, specifies overproof heavy metal species, the present embodiment in salt-soda soil
In the exceeded heavy metal element that detects have pb, cr, hg and Toxic as；
2. the heavy metal species 1. being detected according to step, from corresponding four kinds of slags containing pb, cr, hg, as respectively, to Soviet Union
Cloud gold bacillus cereuss, the red bacterium of basophilic salt, thermophilic saline and alkaline coccus and white rot fungi are tamed, then using standard culture procedures to domestication
Bacterial strain afterwards carries out purification and separation；Described purification process choosing is preferably plate streak, divides 4 areas, and purification cycle is 4
Week, purification 2 times；All kinds of bacterial strains obtaining after purification are separated respectively from each self-corresponding culture medium, that is, obtains described
Aimed strain.
3) apply concretely comprising the following steps of this microbial product:
1. the ratio uniform pressing 10-20kg/ mu in contaminated by heavy metals saline and alkaline ground surface applies microbial product；
2. salt-soda soil is carried out ploughing plough, turn over mixed, and to keep conventional aqueous amount constant irrigation speed in soil periodically to apply water；
3. every 2 months repeat steps 1. -2. once, it is relatively optimum soil that contaminated by heavy metals salt-soda soil is repaired.
The present embodiment carries out compliance test result on the spot in experimental field, and situation is as follows: takes Xinjiang city of province heavy metal pollution salt
Alkali ground as sample plot, takes the soil of more than ten meters of spacing to average after measuring, the ph value in wherein soil is respectively
9.5, as(arsenic) content by weight be 44.25mg/kg, hg(hydrargyrum) content by weight be 0.487mg/kg, pb(lead) content
By weight be 82.46mg/kg, cr(chromium) content by weight be 142.12mg/kg；Through the present embodiment in a manner described should
On target sandy soil, detected in the same fashion again after one month, result is: the ph value in soil is 9, as(arsenic) content with
Weight is calculated as 17.86mg/kg, hg(hydrargyrum) content is 0.178mg/kg by weight, pb(lead) content is by weight
38.16mg/kg, cr(chromium) content by weight be 98.08mg/kg；Again with same after continuing in the same way to administer one month
Sample loading mode is detected, result is: the ph value in soil is 8.5, as(arsenic) content by weight be 11.52mg/kg, hg(hydrargyrum) contain
Amount is 0.12mg/kg by weight, pb(lead) content is 19.99mg/kg by weight, cr(chromium) content is by weight
52.87mg/kg.
National standard is: as(arsenic) content is not more than 15mg/kg, hg(hydrargyrum by weight) content is not more than by weight
0.15mg/kg, pb content is not more than 35mg/kg, cr(chromium by weight) content no more than 90mg/kg by weight.
The operation principle of the present invention is:
Thermophilic saline and alkaline coccus and basophilic salt red bacterium suitable growth ph value are 8-10, suitable with the normal ph value that salt-soda soil is typically dried,
Can muchly survive in salt-soda soil is dried, simultaneously because it belongs to aerobe, will not be due to having in the salt-soda soil after turning over plough
Oxygen environment quick death, makes regulation effect more efficient；After specific domestication, effective strain can more targetedly tolerate to be needed to administer
Heavy metal species in salt-soda soil, can improve enrichment makes antibacterial itself more muchly survive simultaneously；Because white rot fungi is permissible
Quickly crop extremely bad rhizome is decomposed, salt-soda soil fertilizer degree can be increased while reducing and consume, its secretions can simultaneously
Curing heavy metal by way of extracellular precipitation, reduces its harm to plant, and it is hardened so that saline and alkaline soil is difficult, simultaneously its
In decomposition of cellulose Nutrient medium or crop extremely bad rhizome when can produce acidic materials, can improved soil acid-base value, be allowed to gradually go
Salinization of soil；, only with the residue substantially useless to the mankind, Financial cost is low for Nutrient medium.The Nutrient medium of comprehensive nutrition includes to be needed
All nutrients described in oxygen type chmosynthetic heterotrophs antibacterial, simultaneously using be the residue substantially useless to the mankind, for antibacterial
The fertile degree in salt-soda soil, the more conducively improvement on salt-soda soil ground is also add while environment needed for growth is provided；Often the 2-3 month repeat to apply
Plus a microbial product can make the flora sum in salt-soda soil be in higher level all the time, make governance efficiency higher.
The present invention is applied to hydropenia, the salt-soda soil of arid, especially moderate, heavy salinized (ph value 8-10).
Described above to the disclosed embodiments, only for making professional and technical personnel in the field be capable of or using this
Invention.Multiple modifications to these embodiments will be apparent from for those skilled in the art, institute herein
The General Principle of definition can be realized without departing from the spirit or scope of the present invention in other embodiments.Therefore,
The present invention is not intended to be limited to the embodiments shown herein, and is to fit to special with principles disclosed herein and novelty
The consistent scope the widest of point.
Claims (4)
1. a kind of administer heavy metal pollution salt-soda soil microbial product, including Nutrient medium and objective microbe it is characterised in that:
Described Nutrient medium is Powdered nutritional base, including the protide low cost Plant Powder of the ratio mechanical mixture than 5:2:3 with weight
End, energy class low cost plant base material powder, fiber-like low cost plant powder；Described objective microbe is aerobe,
Including bacillus thuringiensiss, the red bacterium of basophilic salt, thermophilic saline and alkaline coccus and white rot fungi.
2. according to claim 1 administer heavy metal pollution salt-soda soil microbial product it is characterised in that: the low one-tenth of protide
This plant base material is preferably Semen Glyciness, and energy class low cost plant base material is preferably Semen Maydiss or Semen arachidis hypogaeae, fiber-like low cost plant base
Material is preferably Testa oryzae or plant straw.
3. the manufacture method of the microbial product in improvement heavy metal pollution salt-soda soil according to any of the above-described claim, its
Step include producing Nutrient medium, training objective bacterial strain, produce microbial product, conserving microorganism product it is characterised in that:
1) described Nutrient medium forming steps are:
1. prepare base material: from protide low cost plant base material, energy class low cost plant base material, fiber-like low cost plant
The each at least one of base material；
2. adopt the oils and fatss that mechanical expression and/or Chemical Leaching extract in base material, the plant residue after deoiling is dried stand-by；
3. using mechanical system, plant residue is clayed into power shape；
4. carry out using the powdery plant residue that 150 DEG C -200 DEG C of temperature previous step obtains that high temperature is degerming and baking removes
Water, you can obtain described Powdered nutritional base；
2) described aimed strain is specially the resistant strain polluting corresponding heavy metal, and its forming steps is:
1. contaminated salt-soda soil is analyzed detecting, specifies overproof heavy metal species in salt-soda soil；
2. the heavy metal species 1. being detected according to step, from entering to bacterial strain of corresponding waste and old heavy metal or heavy metal slag
Row domestication, then using standard culture procedures, purification and separation are carried out to the bacterial strain after domestication；Described purification process choosing is preferably flat
Plate method of scoring, at least divides 4 areas, and purification cycle is 3-4 week, at least purification 2 times；By all kinds of bacterial strains obtaining after purification respectively
Separate from each self-corresponding culture medium, that is, obtain described aimed strain.
4. according to claim 3 administer heavy metal pollution salt-soda soil microbial product manufacture method it is characterised in that:
The method of the oils and fatss in described employing mechanical expression and/or Chemical Leaching extraction base material, is preferably first carried using mechanical expression method
Chemical Leaching method second extraction is adopted again after taking high-quality oils and fatss.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106748531A (en) * | 2017-01-20 | 2017-05-31 | 上海鲲鹏环保科技有限公司 | Alkaline land modifying agent and saline and alkali land improvement method based on spent bleaching clay and plant ash |
| CN106831266A (en) * | 2017-03-31 | 2017-06-13 | 上海立昌环境工程股份有限公司 | Heavy metal pollution alkaline land modifying agent and saline and alkali land improvement method based on spent bleaching clay |
| CN106905052A (en) * | 2017-03-31 | 2017-06-30 | 上海立昌环境工程股份有限公司 | Heavy metal pollution alkaline land modifying agent and saline and alkali land improvement method comprising spent bleaching clay |
| CN106947716A (en) * | 2017-03-31 | 2017-07-14 | 上海立昌环境工程股份有限公司 | The agent of heavy metal pollution Saline-alkali Field Control and saline-alkali soil treatment method comprising spent bleaching clay |
| CN108893423A (en) * | 2018-07-04 | 2018-11-27 | 蒋君怡 | A kind of microbial product and its manufacturing method for administering heavy metal pollution salt-soda soil |
| CN109370598A (en) * | 2018-09-06 | 2019-02-22 | 中海新世纪量子生物科技（深圳）有限责任公司 | Administer leavening, composite bacteria agent, the Preparation method and use in salt-soda soil |
| CN110467924A (en) * | 2019-09-09 | 2019-11-19 | 山东胜伟盐碱地科技有限公司 | A kind of alkaline land soil mercury, cadmium ion remover and preparation method thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106748531A (en) * | 2017-01-20 | 2017-05-31 | 上海鲲鹏环保科技有限公司 | Alkaline land modifying agent and saline and alkali land improvement method based on spent bleaching clay and plant ash |
| CN106831266A (en) * | 2017-03-31 | 2017-06-13 | 上海立昌环境工程股份有限公司 | Heavy metal pollution alkaline land modifying agent and saline and alkali land improvement method based on spent bleaching clay |
| CN106905052A (en) * | 2017-03-31 | 2017-06-30 | 上海立昌环境工程股份有限公司 | Heavy metal pollution alkaline land modifying agent and saline and alkali land improvement method comprising spent bleaching clay |
| CN106947716A (en) * | 2017-03-31 | 2017-07-14 | 上海立昌环境工程股份有限公司 | The agent of heavy metal pollution Saline-alkali Field Control and saline-alkali soil treatment method comprising spent bleaching clay |
| CN108893423A (en) * | 2018-07-04 | 2018-11-27 | 蒋君怡 | A kind of microbial product and its manufacturing method for administering heavy metal pollution salt-soda soil |
| CN109370598A (en) * | 2018-09-06 | 2019-02-22 | 中海新世纪量子生物科技（深圳）有限责任公司 | Administer leavening, composite bacteria agent, the Preparation method and use in salt-soda soil |
| CN110467924A (en) * | 2019-09-09 | 2019-11-19 | 山东胜伟盐碱地科技有限公司 | A kind of alkaline land soil mercury, cadmium ion remover and preparation method thereof |
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Application publication date: 20170118 |