CN106947718A - It is a kind of that the technology that rare-earth tailing improves microbial inoculum is prepared by raw material culture plant-growth promoting rhizobacteria of piggery wastewater - Google Patents
It is a kind of that the technology that rare-earth tailing improves microbial inoculum is prepared by raw material culture plant-growth promoting rhizobacteria of piggery wastewater Download PDFInfo
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- CN106947718A CN106947718A CN201710243566.0A CN201710243566A CN106947718A CN 106947718 A CN106947718 A CN 106947718A CN 201710243566 A CN201710243566 A CN 201710243566A CN 106947718 A CN106947718 A CN 106947718A
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- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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Abstract
The technology that rare-earth tailing improves microbial inoculum is prepared by raw material culture plant-growth promoting rhizobacteria of piggery wastewater the invention provides a kind of, be characterized in using agricultural wastes piggery wastewater as raw material culture plant-growth promoting rhizobacteria, use be stored on June 24th, 2013 deposit number of China typical culture collection center for：CCTCC M 2013283 Bacillus amyloliquefaciens H47 Bacillus amyloliquefaciens strain, commercially available bacillus licheniformis, commercially available bacillus pumilus are plant growth-promoting microbial inoculum, and preparation prepares rare-earth tailing improvement microbial inoculum.Rare-earth tailing improvement microbial inoculum is prepared by raw material culture plant-growth promoting rhizobacteria of piggery wastewater, increases soil fertility using plant growth-promoting fungi degradation ammonium, is alternatively arranged as a kind of water-loss reducer, promotes soil granular structure to be formed, keeps soil from packing together；Soil capillarity is destroyed, soil water evaporation is prevented.Use piggery wastewater for raw material culture plant-growth promoting rhizobacteria, wide material sources are cheap, strain growth rate is fast, and is alternatively arranged as fertilizer, and various nutriments are provided for plant.Technique is simple, and production cost is low, it is easy to promote.So that piggery wastewater is rationally utilized, turn waste into wealth.
Description
Technical field
The present invention relates to the technology for repairing ion type rareearth mine tailing, it is specifically a kind of using agricultural wastes piggery wastewater as
Raw material culture plant-growth promoting rhizobacteria prepares the technology that rare-earth tailing improves microbial inoculum.Belong to biological technical field.
Background technology
The heavy metal pollution of soil is caused by the activity of the mankind mostly.Wherein, the exploitation and smelting of heavy metal are to week
The harm that side soil environment is produced is larger.And the mine tailing produced in the exploitation smelting process of heavy metal, due to processing not in time, it is long
Phase accumulates and rainwater immersion, causes heavy metal to be migrated to Soil Surrounding, can cause extremely serious to the ecological environment residing for it
Heavy metal pollution.Because contaminated soil erosion is big, traditional physics and the restorative procedure of chemistry are because of economic or technical reason
It is difficult to carry out.In recent years, phytoremediation technology is generally believed that low with the incomparable expense of physical chemistry restorative procedure
The honest and clean, secondary pollution that does not destroy treatment of the earth, do not cause underground water, the advantages of beautify the environment, by the extensive weight of the parties concerned
Depending on.So, the screening of hyperaccumulative plant is the Research foundation of phytoremediation technology, with realistic meaning.
The deposit number that this patent is related to is：CCTCC M 2013283 Bacillus amyloliquefaciens
H47 bacillus amyloliquefaciens have good plant growth-promoting characteristic.Rhizosphere plant-growth promoting rhizobacteria can be colonized in root system of plant,
And the bacterium favourable to growth and development of plants.Commercially available bacillus licheniformis and commercially available bacillus pumilus and Bacillus
Amyloliquefaciens H47 composition mix bacterium agents can assist Bacillus amyloliquefaciens H47 to promote plant
Growth.The cost of material of culture plant-growth promoting rhizobacteria is high at present, is unfavorable for the large-scale application of plant growth-promoting microbial inoculum.Therefore grind
Study carefully and set up prepared by raw material culture plant-growth promoting rhizobacteria of piggery wastewater rare-earth tailing improvement microbial inoculum technology it is significant.
Wastewater from pig farm mainly includes pig urine, part pig manure and pig house washing water, belongs to high concentration organic sewage, and suspension
It is big with ammonia-nitrogen content.This raw sewerage enters after natural water, makes solid suspension in water, organic matter and microorganism
Content is raised, and is changed the physics, chemistry and biocoene composition of water body, water quality is degenerated.Also contain substantial amounts of cause of disease in sewage
Microorganism will be diffused propagation by water body or by aquatic animals and plants, endanger human and livestock health.
Most Large-scale pig farms of China are to use flush-type technique.This technics comparing is convenient and swift, still
Simultaneously can be being flushed in waste water together with pig manure, food-residue and pig excrements etc., therefore hanged in waste water containing substantial amounts of solid
Float etc., and other pollutant concentrations are also very high.Pig raising wastewater is a kind of high ammonia nitrogen, high organic concentration, high solid suspension
The organic wastewater of thing concentration, the processing of large-scale pig farm waste water is significant for energy-saving and emission-reduction.
The content of the invention
The present invention prepares the technology that rare-earth tailing improves microbial inoculum by raw material culture plant-growth promoting rhizobacteria of piggery wastewater.To protect
Hiding numbering is：It is CCTCC M 2013283 Bacillus amyloliquefaciens H47 bacillus amyloliquefaciens, commercially available
Bacillus licheniformis and commercially available bacillus pumilus are plant growth-promoting microbial inoculum, are promoted by adding plant after being pre-processed to piggery wastewater
Raw bacterium is enlarged culture, that is, obtains a large amount of ion type rareearth mine tailings for having and improving soil physical chemistry situation, promoting plant growth
The plant growth-promoting microbial inoculum of in-situ immobilization.This method has obvious facilitation to plant in the growth of ionic mine tailing.Give up on pig farm
Water is rationally utilized, and is turned waste into wealth, cheap and simple and easy to do.
Technical scheme：
The deposit number for being stored in China typical culture collection center with June 24th, 2013 is：CCTCC M
2013283 Bacillus amyloliquefaciens H47 bacillus amyloliquefaciens, commercially available bacillus licheniformis is commercially available
Bacillus pumilus mixed plant growth promoting bacteria agent, using piggery wastewater as raw material culture plant-growth promoting rhizobacteria, prepares rare-earth tailing and changes
The technology of good microbial inoculum.Technique is：
1) by Bacillus amyloliquefaciens H47, commercially available of the deposit number for CCTCC M 2013283
The actication of culture such as clothing bacillus, commercially available bacillus pumilus are simultaneously prepared into plant growth-promoting microbial inoculum：
(1) take a ring to be rule on PDA plate respectively in three kinds of strain inclined planes and connect mycelia, 30 DEG C of cultures 18 in incubator
Hour；
(2) three kinds of strain single bacterium colonies of picking are respectively connected in the triangular flask equipped with LB fluid nutrient mediums on PDA plate, accurate
Standby several pieces, shaking table culture, condition of culture：Rotating speed 200r/min, 30 DEG C of temperature incubated 30 hours, that is, obtains plant rush
Bacteria-promoting agent expands the seed of culture.
2) piggery wastewater is pre-processed：Piggery wastewater water content is adjusted to 50-70%, PH to 7.0 is adjusted, mounted in high temperature resistant
Polybag in, 121 DEG C sterilize 30 minutes.
3) in proportion 1 after above-mentioned zymocyte liquid is mixed:In the piggery wastewater culture medium that 10 (v/w) accesses are handled well, protect
Water holding point 50-60%, rises in value 2-3 days under the conditions of 25-30 DEG C, produces the plant-growth promoting rhizobacteria improvement microbial inoculum for expanding culture.
Beneficial effects of the present invention：
1) the rare-earth tailing improvement microbial inoculum that the present invention is prepared using piggery wastewater as raw material culture plant-growth promoting rhizobacteria, Ke Yili
Increase soil fertility with plant growth-promoting fungi degradation ammonium.A kind of water-loss reducer is alternatively arranged as, promotes soil granular structure to be formed, prevents soil
It is hardened；Soil capillarity is destroyed, soil water evaporation is prevented.
2) present invention is raw material culture plant-growth promoting rhizobacteria with piggery wastewater, and wide material sources are cheap, strain propagation speed
Degree is fast, and is alternatively arranged as fertilizer, and various nutriments are provided for plant.
3) rationally utilized, turned waste into wealth the invention enables piggery wastewater.
4) technique of the invention is simple, and production cost is low, it is easy to promote.
Embodiment
Below in conjunction with the embodiment of the present invention, technical scheme is carried out to describe clear, perfectly, it is clear that institute
The embodiment of description is only a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention,
The other embodiment that those of ordinary skill in the art are obtained on the premise of creative work is not made, belongs to the present invention
The scope of protection.
Embodiment 1
1) by Bacillus amyloliquefaciens H47, commercially available of the deposit number for CCTCC M 2013283
The actication of culture such as clothing bacillus, commercially available bacillus pumilus are simultaneously prepared into plant growth-promoting microbial inoculum：
(1) take a ring to be rule on PDA plate respectively in three kinds of strain inclined planes and connect mycelia, 30 DEG C of cultures 18 in incubator
Hour；
(2) three kinds of strain single bacterium colonies of picking are respectively connected to the 250ml equipped with 50ml LB liquid mediums on PDA plate
In triangular flask, each bacterium is inoculated with 12, shaking table culture, condition of culture：Rotating speed 180r/min, 28 DEG C of temperature, incubated 36 is small
When, that is, obtain the seed that plant growth-promoting bacteria agent expands culture.
2) piggery wastewater is pre-processed：Weigh 3 kilograms of piggery wastewaters and adjust water content to 60%, PH to 7.0 is adjusted, mounted in resistance to
In the polybag of high temperature, 121 DEG C sterilize 30 minutes.
3) above-mentioned zymocyte liquid is pressed 1:1:In proportion 1 after 1 mixing:The piggery wastewater culture that 10 (v/w) accesses are handled well
In base, moisture 65% is kept, is rised in value 2-3 days under the conditions of 30 DEG C, the plant-growth promoting rhizobacteria improvement microbial inoculum for expanding culture is produced.
Embodiment 2
1) by Bacillus amyloliquefaciens H47, commercially available of the deposit number for CCTCC M 2013283
The actication of culture such as clothing bacillus, commercially available bacillus pumilus are simultaneously prepared into plant growth-promoting microbial inoculum：
(1) take a ring to be rule on PDA plate respectively in three kinds of strain inclined planes and connect mycelia, 30 DEG C of cultures 20 in incubator
Hour；
(2) three kinds of strain single bacterium colonies of picking are respectively connected to the 250ml equipped with 50ml LB liquid mediums on PDA plate
In triangular flask, each bacterium is inoculated with 12, shaking table culture, condition of culture：Rotating speed 200r/min, 26 DEG C of temperature, incubated 32 is small
When, that is, obtain the seed that plant growth-promoting bacteria agent expands culture.
2) piggery wastewater is pre-processed：Weigh 3 kilograms of piggery wastewaters and adjust water content to 70%, PH to 7.0 is adjusted, mounted in resistance to
In the polybag of high temperature, 121 DEG C sterilize 30 minutes.
3) above-mentioned zymocyte liquid is pressed 1:2:In proportion 1 after 1 mixing:The piggery wastewater culture that 10 (v/w) accesses are handled well
In base, moisture 65% is kept, is rised in value 2-3 days under the conditions of 30 DEG C, the plant-growth promoting rhizobacteria improvement microbial inoculum for expanding culture is produced.
Embodiment 3
1) by Bacillus amyloliquefaciens H47, commercially available of the deposit number for CCTCC M 2013283
The actication of culture such as clothing bacillus, commercially available bacillus pumilus are simultaneously prepared into plant growth-promoting microbial inoculum：
(1) take a ring to be rule on PDA plate respectively in three kinds of strain inclined planes and connect mycelia, 30 DEG C of cultures 22 in incubator
Hour；
(2) three kinds of strain single bacterium colonies of picking are respectively connected to the 250ml equipped with 50ml LB liquid mediums on PDA plate
In triangular flask, each bacterium is inoculated with 12, shaking table culture, condition of culture：Rotating speed 160r/min, 32 DEG C of temperature, incubated 28 is small
When, that is, obtain the seed that plant growth-promoting bacteria agent expands culture.
2) piggery wastewater is pre-processed：Weigh 3 kilograms of piggery wastewaters and adjust water content to 65%, PH to 7.0 is adjusted, mounted in resistance to
In the polybag of high temperature, 121 DEG C sterilize 30 minutes.
3) above-mentioned zymocyte liquid is pressed 1:1:In proportion 1 after 2 mixing:The piggery wastewater culture that 10 (v/w) accesses are handled well
In base, moisture 60% is kept, is rised in value 2-3 days under the conditions of 30 DEG C, the plant-growth promoting rhizobacteria improvement microbial inoculum for expanding culture is produced.
Embodiment 4
1) by Bacillus amyloliquefaciens H47, commercially available of the deposit number for CCTCC M 2013283
The actication of culture such as clothing bacillus, commercially available bacillus pumilus are simultaneously prepared into plant growth-promoting microbial inoculum：
(1) take a ring to be rule on PDA plate respectively in three kinds of strain inclined planes and connect mycelia, 30 DEG C of cultures 16 in incubator
Hour；
(2) three kinds of strain single bacterium colonies of picking are respectively connected to the 250ml equipped with 50ml LB liquid mediums on PDA plate
In triangular flask, each bacterium is inoculated with 12, shaking table culture, condition of culture：Rotating speed 200r/min, 25 DEG C of temperature, incubated 36 is small
When, that is, obtain the seed that plant growth-promoting bacteria agent expands culture.
2) piggery wastewater is pre-processed：Weigh 3 kilograms of piggery wastewaters and adjust water content to 65%, PH to 7.0 is adjusted, mounted in resistance to
In the polybag of high temperature, 121 DEG C sterilize 30 minutes.
3) above-mentioned zymocyte liquid is pressed 2:1:In proportion 1 after 1 mixing:The piggery wastewater culture that 10 (v/w) accesses are handled well
In base, moisture 55% is kept, is rised in value 2-3 days under the conditions of 30 DEG C, the plant-growth promoting rhizobacteria improvement microbial inoculum for expanding culture is produced.
In the present invention, the ratios of Mixed Microbes, inoculum concentration, piggery wastewater moisture content in medium etc. are not limited to embodiment and lifted
Combination, different combinations can be selected according to actual conditions.
The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention, all thinkings in the present invention
Within principle, any modifications, equivalent substitutions and improvements done etc. should be included within the scope of the present invention.
Claims (4)
1. a kind of prepare the technology that rare-earth tailing improves microbial inoculum by raw material culture plant-growth promoting rhizobacteria of piggery wastewater, it is characterized in
Using agricultural wastes piggery wastewater as raw material culture plant-growth promoting rhizobacteria, Chinese Typical Representative culture was stored in using on June 24th, 2013
The deposit number of thing collection is：CCTCC M 2013283 Bacillus amyloliquefaciens H47 Xie Dian
Afnyloliquefaciens bacterial strain, commercially available bacillus licheniformis, commercially available bacillus pumilus are plant growth-promoting microbial inoculum, and preparation prepares rare earth tail
Ore deposit improves microbial inoculum, and its technique is：
1) Bacillus amyloliquefaciens H47 by deposit number for CCTCC M 2013283, commercially available lichens bud
The actication of culture such as spore bacillus, commercially available bacillus pumilus are simultaneously prepared into plant growth-promoting microbial inoculum：
(1) take a ring to be rule on PDA plate respectively in three kinds of strain inclined planes and connect mycelia, 30 DEG C of cultures 18 are small in incubator
When；
(2) three kinds of strain single bacterium colonies of picking are respectively connected in the triangular flask equipped with LB fluid nutrient mediums on PDA plate, if preparing
Dry part, shaking table culture, condition of culture：Rotating speed 200r/min, 30 DEG C of temperature incubated 30 hours, that is, obtains plant growth-promoting bacteria
Agent expands the seed of culture.
2) piggery wastewater is pre-processed：Piggery wastewater water content is adjusted to 50-70%, PH to 7.0 is adjusted, mounted in resistant to elevated temperatures modeling
In pocket, 121 DEG C sterilize 30 minutes.
3) in proportion 1 after above-mentioned zymocyte liquid is mixed:In the piggery wastewater culture medium that 10 (v/w) accesses are handled well, water is kept
Divide 50-60%, rise in value 2-3 days under the conditions of 25-30 DEG C, produce the plant-growth promoting rhizobacteria improvement microbial inoculum for expanding culture.
2. a kind of according to claim 1 prepare rare-earth tailing improvement bacterium by raw material culture plant-growth promoting rhizobacteria of piggery wastewater
The technology of agent, it is characterised in that：Plant growth-promoting bacteria deposit number is：CCTCC M 2013283.
3. a kind of according to claim 1 prepare rare-earth tailing improvement bacterium by raw material culture plant-growth promoting rhizobacteria of piggery wastewater
The technology of agent, it is characterised in that：Using agricultural wastes piggery wastewater as raw material culture plant-growth promoting rhizobacteria.
4. a kind of according to claim 1 prepare rare-earth tailing improvement bacterium by raw material culture plant-growth promoting rhizobacteria of piggery wastewater
The technology of agent, it is characterised in that：The plant growth-promoting microbial inoculum is bacillus amyloliquefaciens, commercially available bacillus licheniformis, commercially available short and small
The mix bacterium agent of bacillus composition.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109020644A (en) * | 2018-09-05 | 2018-12-18 | 河北大学 | Utilize the method and application of Pig raising wastewater fermenting and producing microbial manure |
| CN109456091A (en) * | 2018-09-05 | 2019-03-12 | 河北大学 | Utilize the method and application of cowboying wastewater fermentation production microbial manure |
| CN109526536A (en) * | 2017-07-31 | 2019-03-29 | 张家港爱尔乐信息科技有限公司 | A kind of stalk fermentation bed Inoculant and preparation method thereof and application method |
| CN115232771A (en) * | 2022-07-29 | 2022-10-25 | 福州文泽生物科技有限公司 | Rare earth ammonia nitrogen treatment microbial agent and culture method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109526536A (en) * | 2017-07-31 | 2019-03-29 | 张家港爱尔乐信息科技有限公司 | A kind of stalk fermentation bed Inoculant and preparation method thereof and application method |
| CN109020644A (en) * | 2018-09-05 | 2018-12-18 | 河北大学 | Utilize the method and application of Pig raising wastewater fermenting and producing microbial manure |
| CN109456091A (en) * | 2018-09-05 | 2019-03-12 | 河北大学 | Utilize the method and application of cowboying wastewater fermentation production microbial manure |
| CN115232771A (en) * | 2022-07-29 | 2022-10-25 | 福州文泽生物科技有限公司 | Rare earth ammonia nitrogen treatment microbial agent and culture method |
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