CN105149339A - Method for preparing ionic rare earth mine tailing in-situ remediation plant nursing system - Google Patents
Method for preparing ionic rare earth mine tailing in-situ remediation plant nursing system Download PDFInfo
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- CN105149339A CN105149339A CN201510125409.0A CN201510125409A CN105149339A CN 105149339 A CN105149339 A CN 105149339A CN 201510125409 A CN201510125409 A CN 201510125409A CN 105149339 A CN105149339 A CN 105149339A
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Abstract
The invention provides a method for preparing an ionic rare earth mine tailing in-situ remediation plant nursing system. The method utilizes agricultural waste as a biomass carrier and a Bacillus amyloliquefaciens H47 strain as a plant growth promoting inocula which is preserved in the China center for type culture collection on June 24, 2013 and has a preservation number of CCTCC NO: M2013283 to prepare the ionic rare earth mine tailing in-situ remediation plant nursing system. Through immobilization of the biomass carrier, activity of the plant growth promoting inocula can be kept for a long time and the plant growth promoting inocula can produce stable and efficient action in a complex environment. The system can improve soil fertility and prevents soil hardening and fast soil moisture evaporation. The system utilizes the agricultural waste biomass carrier and has a wide source, a low cost and simple processes. The system realizes waste utilization, can be used as bio-fertilizer, and provides various nutrients for a plant. Through the system, the restored soil is suitable for plant growth.
Description
Technical field
The present invention relates to the technology of repairing ion type rareearth mine tailing, a kind of technology taking agricultural wastes as biomass carrier immobilization plant growth-promoting microbial inoculum and prepare plant-nursing system in-situ immobilization rare-earth tailing specifically.Belong to biological technical field.
Background technology
Pond leaching process and dump leaching method technique and the lighter in_situ leaching method of uncontamination is employed on a large scale in ion type rareearth exploitation.A large amount of NH residual in rare-earth tailing
4+, SO
4 2-cause soil acidification and harden, and residual rare earth element, all may produce harm to the growth of plant, cause vegetation on rare-earth tailing to lack.Due to the most air storage of rare-earth tailing, vegetation lacks in addition, severe water and soil erosion, thus Agro-ecology in heavy damage region.Research shows, one of best mode that mine tailing is administered is exactly revegetation, and the plant of normal selection high-biomass is used for revegetation to repair mine tailing soil.For can to realize reclaiming, often utilize microorganism remediation technology to improve mining soil fertility and biological activity of soil by Planting Crops on mine tailing.
Plant-growth promoting rhizobacteria generally screens from the rhizosphere of resistance to adversity plant, and is used for the growth-promoting bacterium of screening improving the ability that other plant adapts to similar adverse circumstance.And the rare-earth tailing soil physico-chemical property exploited for a long time has gone to pot, vegetation is rare, is difficult to select suitable plant rhizosphere for screening growth-promoting bacterium from it.Therefore, from microbial resources widely, growth-promoting bacterium is screened very necessary.Because rare-earth tailing is to the harm of plant mainly drought stress, soil acidification and high NH
4+coerce the biological effectiveness reduction that the iron ion indirectly caused with rare earth element absorbs suppression and phosphorus.The ability should for the growth-promoting bacterium of repairing rare-earth tailing with stronger osmophilic strain, low pH environment of therefore screening, and can promote that plant is to the absorption of iron ion and P elements.
Honey is a kind of oversaturated acid sugar solution, and water activity is low, and osmotic pressure is high, and containing a small amount of H
2o
2.Bacillus in honey generally has the ability that stronger osmophilic strain, low pH and high oxidation are coerced.Bacillus in more existing honey is applied to the control of phytopathogen and the bibliographical information as probio.The ability of the resistance to adverse circumstance of the height that bacillus has will improve its survival ability in the adverse circumstance environment that rare-earth tailing is similar, and the deposit number that this patent relates to is: the bacillus amyloliquefaciens of the BacillusamyloliquefaciensH47 of CCTCCNO:M2013283 has good plant growth-promoting characteristic.But bacterial strain fixation support general at present is with high costs, is unfavorable for the large-scale application of plant growth-promoting microbial inoculum.Therefore study and set up with agricultural wastes is that the biomass carrier of plant growth-promoting microbial inoculum is significant.
Summary of the invention
The present invention is using agricultural wastes as the immobilized biomass carrier of plant growth-promoting microbial inoculum.With deposit number be: the bacillus amyloliquefaciens of the BacillusamyloliquefaciensH47 of CCTCCNO:M2013283 is plant growth-promoting microbial inoculum, by namely obtaining a kind of only there is the plant-nursing system of ion type rareearth mine tailing in-situ immobilization improving soil physical chemistry situation, Promoting plant growth to adding the plant growth-promoting microbial inoculum mixing spread cultivation after the pretreatment of agricultural wastes.The method has obvious facilitation to plant in the growth of ionic mine tailing.Appropriate application is obtained in agricultural wastes, cheap and can turn waste into wealth, simple and easy to do.
Technical scheme of the present invention:
With the deposit number that on June 24th, 2013 is stored in Wuhan, China Wuhan University China typical culture collection center be: the bacillus amyloliquefaciens plant growth-promoting microbial inoculum main body of the BacillusamyloliquefaciensH47 of CCTCCNO:M2013283, with through pretreated agricultural wastes for biomass carrier, prepare ion type rareearth mine tailing in-situ immobilization plant-nursing system.Technique is:
1) by deposit number be CCTCCNO:M2013283 BacillusamyloliquefaciensH47 actication of culture and be prepared into plant growth-promoting microbial inoculum:
(1) get slant strains one ring and connect mycelia at the flat lining out of PDA, cultivate 48 hours for 30 DEG C in incubator;
(2) on picking PDA flat board, single bacterium colony access is equipped with in the triangular flask of nutrient solution, and prepare some parts, be placed in shaking table, condition of culture: rotating speed 200rpm, temperature 30 DEG C, constant temperature culture 3-5 days, namely obtains plant growth-promoting microbial inoculum.
2) biomass carrier pretreatment: according to mass ratio: add water to water content 30-45% after the mixing of 60-80% agricultural waste material, 10-25% blackstrap, 2-3% beancake powder, regulate PH to 7.0, be contained in resistant to elevated temperatures polybag, 121 DEG C of sterilizings 30 minutes.
3) by above-mentioned zymocyte liquid in proportion 1: 10 (v/w) access in the biomass carrier handled well, keep moisture 40-60%, rise in value 4-7 days under 25-30 DEG C of condition, obtains plant-nursing system.
Beneficial effect of the present invention:
1) what prepared by the present invention utilizes agricultural wastes as plant growth-promoting microbial inoculum biomass carrier plant-nursing system, and plant growth-promoting fungi degradation ammonium can be utilized to increase soil fertility.Also can be used as a kind of water-loss reducer, promote that soil aggregate is formed, keep soil from packing together; Spoiled soil capillarity, prevents soil water evaporation.
2) the present invention selects agricultural wastes as the biomass carrier of plant growth-promoting microbial inoculum, makes bacterial classification stay active for long periods, can play consistently high efficiency under complicated environmental condition.
3) the present invention agricultural wastes are as fixation support, and wide material sources are cheap, advantages of good adsorption effect, and bacterial classification growth rate is fast.But also can be used as fertilizer, for plant provides various nutriment.
4) the present invention is as the technical support of phytoremediation rare-earth tailing, lower than physical-chemical process disposal cost, little to ambient influnence, efficiency is high, and can not cause secondary pollution
5) technique of the present invention is simple, and production cost is low.
Detailed description of the invention
Embodiment 1
1) by deposit number be CCTCCNO:M2013283 BacillusamyloliquefaciensH47 actication of culture and be prepared into plant growth-promoting microbial inoculum:
(1) get slant strains one ring and connect mycelia at the flat lining out of PDA, cultivate 48 hours for 30 DEG C in incubator;
(2) on picking PDA flat board, the dull and stereotyped single bacterium colony access of single bacterium colony access is equipped with in the 250ml triangular flask of 50ml nutrient solution, and prepare 9 with rotating speed 200rpm on shaking table, the condition constant temperature culture of temperature 30 DEG C 5 days, obtains plant growth-promoting microbial inoculum.
2) biomass carrier pretreatment: get 3 kilograms of rice husks, according to mass ratio: add water to water content 40% after 60% rice husk, 20% blackstrap, 3% beancake powder mixing, regulate PH to 7.0, be contained in resistant to elevated temperatures polybag, 121 DEG C of sterilizings 30 minutes.
3) by above-mentioned zymocyte liquid in proportion 1: 10 (v/w) access in the biomass carrier handled well, keep moisture 45%, rise in value 4-7 days under 30 DEG C of conditions, obtains plant-nursing system.
Claims (3)
1. prepare the method for ion type rareearth mine tailing in-situ immobilization plant-nursing system for one kind, be characterized in taking agricultural wastes as biomass carrier, the deposit number adopting on June 24th, 2013 to be stored in Wuhan University's China typical culture collection center is: the Bacillus amyloliquefaciens strain of the BacillusamyloliquefaciensH47 of CCTCCNO:M2013283 is plant growth-promoting microbial inoculum, prepare ion type rareearth mine tailing in-situ immobilization plant-nursing system
Its technique is:
1) by deposit number be CCTCCNO:M2013283 BacillusamyloliquefaciensH47 actication of culture and be prepared into plant growth-promoting microbial inoculum:
(1) get slant strains one ring and connect mycelia at the flat lining out of PDA, cultivate 48 hours for 30 DEG C in incubator;
(2) on picking PDA flat board, single bacterium colony access is equipped with in the triangular flask of nutrient solution, and be placed in shaking table, condition of culture: rotating speed 200rpm, temperature 30 DEG C, constant temperature culture 3-5 days, namely obtains plant growth-promoting microbial inoculum;
2) biomass carrier pretreatment: according to mass ratio: add water to water content 40-55% after the mixing of 60-80% agricultural waste material, 10-25% blackstrap, 2-3% beancake powder, regulate PH to 7.0, be contained in resistant to elevated temperatures polybag, 121 DEG C of sterilizings 30 minutes;
3) by above-mentioned zymocyte liquid in proportion 1: 10 (v/w) access in the biomass carrier handled well, keep moisture 40-55%, rise in value 4-7 days under 25-30 DEG C of condition, obtains plant-nursing system.
2. a kind of method preparing ion type rareearth mine tailing in-situ immobilization plant-nursing system according to claim 1, is characterized in that: plant growth-promoting bacteria deposit number is: CCTCCNO:M2013283.
3. a kind of method preparing ion type rareearth mine tailing in-situ immobilization plant-nursing system according to claim 1, is characterized in that: agricultural wastes comprise the plant fibrous discarded object such as stalk, rice husk, straw, wheat bran, corncob, peanut shell and the waste material of animality such as feces of livestock and poultry, feed manufacturing leftover bits and pieces.
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| CN201510125409.0A CN105149339A (en) | 2015-03-23 | 2015-03-23 | Method for preparing ionic rare earth mine tailing in-situ remediation plant nursing system |
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| CN201510125409.0A CN105149339A (en) | 2015-03-23 | 2015-03-23 | Method for preparing ionic rare earth mine tailing in-situ remediation plant nursing system |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106867948A (en) * | 2017-04-14 | 2017-06-20 | 江南大学 | It is a kind of to prepare the technology that rare-earth tailing improves microbial inoculum by raw material culture plant-growth promoting rhizobacteria of lactose refining waste water |
| CN106947717A (en) * | 2017-04-14 | 2017-07-14 | 江南大学 | It is a kind of that the technology that rare-earth tailing improves microbial inoculum is prepared by raw material culture plant-growth promoting rhizobacteria of pig manure |
| CN107790490A (en) * | 2016-08-31 | 2018-03-13 | 河北施可丰生物科技有限公司 | A kind of microbial method ion exchange soil restoring technology |
| CN108114976A (en) * | 2017-12-19 | 2018-06-05 | 轻工业环境保护研究所 | A kind of method of repairing polluted soil |
| CN110512619A (en) * | 2019-08-08 | 2019-11-29 | 中国恩菲工程技术有限公司 | Ecological remediation type rare-earth tailing and reparation rare-earth tailing method |
| CN110540841A (en) * | 2019-05-21 | 2019-12-06 | 中节能大地环境修复有限公司 | Surface curing agent and curing method for ionic rare earth in-situ leaching abandoned mine |
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| CN103451128A (en) * | 2013-07-12 | 2013-12-18 | 江南大学 | Bacillus amyloliquefaciens and application thereof in preventing and controlling peach bleeding disease |
| CN103920706A (en) * | 2014-04-16 | 2014-07-16 | 内蒙古科技大学 | Method for repairing rare earth polluted soil by immobilized mixed fungicide |
| CN103966125A (en) * | 2014-04-25 | 2014-08-06 | 王夕刚 | Bacillus licheniformis, microbial agent and application of bacillus licheniformis or microbial agent in mine ecological remediation |
| WO2014152350A1 (en) * | 2013-03-15 | 2014-09-25 | Angel Janet | Composition and methods of use |
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| CN102839142A (en) * | 2012-09-14 | 2012-12-26 | 杨凌绿都生物科技有限公司 | Bacillus amyloliquefaciens (Ba 168) and fermenting culture method and application of bacillus amyloliquefaciens |
| WO2014152350A1 (en) * | 2013-03-15 | 2014-09-25 | Angel Janet | Composition and methods of use |
| CN103451128A (en) * | 2013-07-12 | 2013-12-18 | 江南大学 | Bacillus amyloliquefaciens and application thereof in preventing and controlling peach bleeding disease |
| CN103920706A (en) * | 2014-04-16 | 2014-07-16 | 内蒙古科技大学 | Method for repairing rare earth polluted soil by immobilized mixed fungicide |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107790490A (en) * | 2016-08-31 | 2018-03-13 | 河北施可丰生物科技有限公司 | A kind of microbial method ion exchange soil restoring technology |
| CN107790490B (en) * | 2016-08-31 | 2020-09-15 | 山东金赛生态农业有限公司 | Microbial method ion exchange soil remediation technology |
| CN106867948A (en) * | 2017-04-14 | 2017-06-20 | 江南大学 | It is a kind of to prepare the technology that rare-earth tailing improves microbial inoculum by raw material culture plant-growth promoting rhizobacteria of lactose refining waste water |
| CN106947717A (en) * | 2017-04-14 | 2017-07-14 | 江南大学 | It is a kind of that the technology that rare-earth tailing improves microbial inoculum is prepared by raw material culture plant-growth promoting rhizobacteria of pig manure |
| CN108114976A (en) * | 2017-12-19 | 2018-06-05 | 轻工业环境保护研究所 | A kind of method of repairing polluted soil |
| CN108114976B (en) * | 2017-12-19 | 2021-10-15 | 轻工业环境保护研究所 | Method for restoring polluted soil |
| CN110540841A (en) * | 2019-05-21 | 2019-12-06 | 中节能大地环境修复有限公司 | Surface curing agent and curing method for ionic rare earth in-situ leaching abandoned mine |
| CN110512619A (en) * | 2019-08-08 | 2019-11-29 | 中国恩菲工程技术有限公司 | Ecological remediation type rare-earth tailing and reparation rare-earth tailing method |
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