CN106947717A - It is a kind of that the technology that rare-earth tailing improves microbial inoculum is prepared by raw material culture plant-growth promoting rhizobacteria of pig manure - Google Patents
It is a kind of that the technology that rare-earth tailing improves microbial inoculum is prepared by raw material culture plant-growth promoting rhizobacteria of pig manure Download PDFInfo
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- CN106947717A CN106947717A CN201710243533.6A CN201710243533A CN106947717A CN 106947717 A CN106947717 A CN 106947717A CN 201710243533 A CN201710243533 A CN 201710243533A CN 106947717 A CN106947717 A CN 106947717A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
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Abstract
The technology that rare-earth tailing improves microbial inoculum is prepared by raw material culture plant-growth promoting rhizobacteria of pig manure the invention provides a kind of, be characterized in using agricultural wastes pig manure as raw material culture plant-growth promoting rhizobacteria, use be stored on June 24th, 2013 deposit number of China typical culture collection center for：CCTCC M 2013283 Bacillus amyloliquefaciens H47 Bacillus amyloliquefaciens strain, commercially available bacillus licheniformis, commercially available bacillus pumilus are plant growth-promoting microbial inoculum, and preparation prepares rare-earth tailing improvement microbial inoculum.Rare-earth tailing improvement microbial inoculum is prepared by raw material culture plant-growth promoting rhizobacteria of pig manure, increases soil fertility using plant growth-promoting fungi degradation ammonium, is alternatively arranged as a kind of water-loss reducer, promotes soil granular structure to be formed, keeps soil from packing together；Soil capillarity is destroyed, soil water evaporation is prevented.Use pig manure for raw material culture plant-growth promoting rhizobacteria, wide material sources are cheap, strain growth rate is fast, and is alternatively arranged as fertilizer, and various nutriments are provided for plant.Technique is simple, and production cost is low, it is easy to promote.So that pig manure is rationally utilized, turn waste into wealth.
Description
Technical field
The present invention relates to the technology for repairing ion type rareearth mine tailing, specifically one kind is using agricultural wastes pig manure as raw material
Culture plant-growth promoting rhizobacteria prepares the technology that rare-earth tailing improves microbial inoculum.Belong to biological technical field.
Background technology
In recent years, to the illegal excessive coyoting of rare-earth ore resource, the ecological environment problem in mining area is made to become increasingly conspicuous, in rare earth
In mining process, surface soil and vegetation are stripped, and soil washes pouring by the serious of strong acid so that nutrient significantly drops in soil
Low, some vegetation are difficult to the long-term soil environment for adapting to strong acid and low nutrient.The accumulation of Rare Earthelements In The Soils also causes soil
Fertility decline, soil desertification, the ecosystem are fragile, and revegetation and the recovery of rare earth abandoned mine land are particularly important.
The deposit number that this patent is related to is：CCTCC M 2013283 Bacillus amyloliquefaciens
H47 bacillus amyloliquefaciens have good plant growth-promoting characteristic.Rhizosphere plant-growth promoting rhizobacteria can be colonized in root system of plant,
And the bacterium favourable to growth and development of plants.Commercially available bacillus licheniformis and commercially available bacillus pumilus and Bacillus
Amyloliquefaciens H47 composition mix bacterium agents can assist Bacillus amyloliquefaciens H47 to promote plant
Growth.The cost of material of culture plant-growth promoting rhizobacteria is high at present, is unfavorable for the large-scale application of plant growth-promoting microbial inoculum.Therefore grind
Study carefully and set up prepared by raw material culture plant-growth promoting rhizobacteria of pig manure rare-earth tailing improvement microbial inoculum technology it is significant.
The food-safe attention of increase and people with the market demand, pig industry is gradually from free-ranging to intensive
Development, total size has also obtained rapid increase, and then increases pig industry excreta rapidly.Swine manure etc. is to water
Body, air and Environmental Health bring immense pressure.The foul gas of pig manure mainly includes ammonia and hydrogen sulfide, and it is mainly because of pig pair
Protein feeds utilization rate is low to be caused, remaining anaerobic fermentation of the nutriment through microorganism in excrement and produce ammonia and vulcanization
Hydrogen, and it is harmful further can to produce phenol, scatol, ethanol, mercaptan, acetaldehyde, volatile fatty acid etc. with the effect of other materials
Material, reduces air quality, is detrimental to health.The sewage that generation is washed away on pig manure and pig farm contains N, P, K and large amount of organic,
The harmful substances such as compound, antibiotic medicine, pathogenic microorganism containing substantial amounts of copper, zinc, arsenic etc., if directly discharging, easily
It direct polluted surface water, can also permeate the ground, cause body eutrophication, influence crops, soil to be dwelt biological growth and ecological peace
Entirely.Pig manure contains the pathogenic microorganisms such as a certain amount of campylobacter, salmonella, Escherichia coli, parasitic ovum, the mosquitos and flies of evil life
Deng, discharged if unprocessed, and use, ecological environment can be entered, or even can enter in crop body, finally give the mankind and livestock and poultry
Bring more harm.
Influence of the known pig manure to environment is mainly acted as because of organic matter, heavy metal ion, mosquito ovum and pathogenic microorganism
With.Many of which is the nutriment required for rhizosphere growth-promoting endophytic bacteria cell growth, and other pollutants can then be turned by growth-promoting bacterium
Change.Therefore, it can with pig manure is raw material culture plant-growth promoting rhizobacteria, for improveing rare-earth tailing, can both remove pig manure pollution,
Heavy metals soil can be repaired again, is improved the ecological environment, and promoted the well-being of mankind.
The content of the invention
The present invention prepares the technology that rare-earth tailing improves microbial inoculum by raw material culture plant-growth promoting rhizobacteria of pig manure.Compiled with preservation
Number it is：CCTCC M 2013283 Bacillus amyloliquefaciens H47 bacillus amyloliquefaciens, commercially available lichens
Bacillus and commercially available bacillus pumilus are plant growth-promoting microbial inoculum, are carried out by adding plant growth-promoting bacteria after being pre-processed to pig manure
Expand culture, that is, obtain a large amount of ion type rareearth mine tailing in-situ immobilizations for having and improving soil physical chemistry situation, promoting plant growth
Plant growth-promoting microbial inoculum.This method has obvious facilitation to plant in the growth of ionic mine tailing.Pig manure obtains reasonable profit
With, turn waste into wealth, it is cheap and simple and easy to do.
Technical scheme：
The deposit number for being stored in China typical culture collection center with June 24th, 2013 is：CCTCC M
2013283 Bacillus amyloliquefaciens H47 bacillus amyloliquefaciens, commercially available bacillus licheniformis is commercially available
Bacillus pumilus mixed plant growth promoting bacteria agent, using pig manure as raw material culture plant-growth promoting rhizobacteria, prepares rare-earth tailing improvement bacterium
The technology of agent.Technique is：
1) by Bacillus amyloliquefaciens H47, commercially available of the deposit number for CCTCC M 2013283
The actication of culture such as clothing bacillus, commercially available bacillus pumilus are simultaneously prepared into plant growth-promoting microbial inoculum：
(1) take a ring to be rule on PDA plate respectively in three kinds of strain inclined planes and connect mycelia, 30 DEG C of cultures 20 in incubator
Hour；
(2) three kinds of strain single bacterium colonies of picking are respectively connected in the triangular flask equipped with LB fluid nutrient mediums on PDA plate, accurate
Standby several pieces, shaking table culture, condition of culture：Rotating speed 200r/min, 30 DEG C of temperature incubated 36 hours, that is, obtains plant rush
Bacteria-promoting agent expands the seed of culture.
2) pig manure is pre-processed：Pig manure adds water to water content 40-60%, PH to 7.0 is adjusted, mounted in resistant to elevated temperatures polybag
In, 121 DEG C sterilize 30 minutes.
3) in proportion 1 after above-mentioned zymocyte liquid is mixed:In the pig manure culture medium that 10 (v/w) accesses are handled well, water is kept
Divide 40-60%, rise in value 2-3 days under the conditions of 25-30 DEG C, produce the plant-growth promoting rhizobacteria improvement microbial inoculum for expanding culture.
Beneficial effects of the present invention：
1) the rare-earth tailing improvement microbial inoculum that the present invention is prepared using pig manure as raw material culture plant-growth promoting rhizobacteria, it is possible to use plant
Thing growth-promoting fungi degradation ammonium increases soil fertility.A kind of water-loss reducer is alternatively arranged as, promotes soil granular structure to be formed, prevents soil plate
Knot；Soil capillarity is destroyed, soil water evaporation is prevented.
2) present invention is raw material culture plant-growth promoting rhizobacteria with pig manure, and wide material sources are cheap, and strain growth rate is fast,
And fertilizer is alternatively arranged as, provide various nutriments for plant.
3) rationally utilized, turned waste into wealth the invention enables pig manure.
4) technique of the invention is simple, and production cost is low, it is easy to promote.
Embodiment
Below in conjunction with the embodiment of the present invention, technical scheme is carried out to describe clear, perfectly, it is clear that institute
The embodiment of description is only a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention,
The other embodiment that those of ordinary skill in the art are obtained on the premise of creative work is not made, belongs to the present invention
The scope of protection.
Embodiment 1
1) by Bacillus amyloliquefaciens H47, commercially available of the deposit number for CCTCC M 2013283
The actication of culture such as clothing bacillus, commercially available bacillus pumilus are simultaneously prepared into plant growth-promoting microbial inoculum：
(1) take a ring to be rule on PDA plate respectively in three kinds of strain inclined planes and connect mycelia, 30 DEG C of cultures 18 in incubator
Hour；
(2) three kinds of strain single bacterium colonies of picking are respectively connected to the 250ml equipped with 50ml LB liquid mediums on PDA plate
In triangular flask, each bacterium is inoculated with 12, shaking table culture, condition of culture：Rotating speed 180r/min, 30 DEG C of temperature, incubated 30 is small
When, that is, obtain the seed that plant growth-promoting bacteria agent expands culture.
2) pig manure is pre-processed：Weigh 3 kilograms of pig manures and add water to water content 60%, PH to 7.0 is adjusted, mounted in resistant to elevated temperatures modeling
In pocket, 121 DEG C sterilize 30 minutes.
3) above-mentioned zymocyte liquid is pressed 1:1:In proportion 1 after 1 mixing:In the pig manure culture medium that 10 (v/w) accesses are handled well,
Moisture 55% is kept, is rised in value 2-3 days under the conditions of 30 DEG C, the plant-growth promoting rhizobacteria improvement microbial inoculum for expanding culture is produced.
Embodiment 2
1) by Bacillus amyloliquefaciens H47, commercially available of the deposit number for CCTCC M 2013283
The actication of culture such as clothing bacillus, commercially available bacillus pumilus are simultaneously prepared into plant growth-promoting microbial inoculum：
(1) take a ring to be rule on PDA plate respectively in three kinds of strain inclined planes and connect mycelia, 28 DEG C of cultures 20 in incubator
Hour；
(2) three kinds of strain single bacterium colonies of picking are respectively connected to the 250ml equipped with 50ml LB liquid mediums on PDA plate
In triangular flask, each bacterium is inoculated with 12, shaking table culture, condition of culture：Rotating speed 200r/min, 32 DEG C of temperature, incubated 48 is small
When, that is, obtain the seed that plant growth-promoting bacteria agent expands culture.
2) pig manure is pre-processed：Weigh 3 kilograms of pig manures and add water to water content 50%, PH to 7.0 is adjusted, mounted in resistant to elevated temperatures modeling
In pocket, 121 DEG C sterilize 30 minutes.
3) above-mentioned zymocyte liquid is pressed 1:2:In proportion 1 after 1 mixing:In the pig manure culture medium that 10 (v/w) accesses are handled well,
Moisture 45% is kept, is rised in value 2-3 days under the conditions of 30 DEG C, the plant-growth promoting rhizobacteria improvement microbial inoculum for expanding culture is produced.
Embodiment 3
1) by Bacillus amyloliquefaciens H47, commercially available of the deposit number for CCTCC M 2013283
The actication of culture such as clothing bacillus, commercially available bacillus pumilus are simultaneously prepared into plant growth-promoting microbial inoculum：
(1) take a ring to be rule on PDA plate respectively in three kinds of strain inclined planes and connect mycelia, 30 DEG C of cultures 24 in incubator
Hour；
(2) three kinds of strain single bacterium colonies of picking are respectively connected to the 250ml equipped with 50ml LB liquid mediums on PDA plate
In triangular flask, each bacterium is inoculated with 12, shaking table culture, condition of culture：Rotating speed 240r/min, 32 DEG C of temperature, incubated 28 is small
When, that is, obtain the seed that plant growth-promoting bacteria agent expands culture.
2) pig manure is pre-processed：Weigh 3 kilograms of pig manures and add water to water content 55%, PH to 7.0 is adjusted, mounted in resistant to elevated temperatures modeling
In pocket, 121 DEG C sterilize 30 minutes.
3) above-mentioned zymocyte liquid is pressed 1:1:In proportion 1 after 2 mixing:In the pig manure culture medium that 10 (v/w) accesses are handled well,
Moisture 50% is kept, is rised in value 2-3 days under the conditions of 30 DEG C, the plant-growth promoting rhizobacteria improvement microbial inoculum for expanding culture is produced.
Embodiment 4
1) by Bacillus amyloliquefaciens H47, commercially available of the deposit number for CCTCC M 2013283
The actication of culture such as clothing bacillus, commercially available bacillus pumilus are simultaneously prepared into plant growth-promoting microbial inoculum：
(1) take a ring to be rule on PDA plate respectively in three kinds of strain inclined planes and connect mycelia, 30 DEG C of cultures 16 in incubator
Hour；
(2) three kinds of strain single bacterium colonies of picking are respectively connected to the 250ml equipped with 50ml LB liquid mediums on PDA plate
In triangular flask, each bacterium is inoculated with 12, shaking table culture, condition of culture：Rotating speed 200r/min, 26 DEG C of temperature, incubated 38 is small
When, that is, obtain the seed that plant growth-promoting bacteria agent expands culture.
2) pig manure is pre-processed：Weigh 3 kilograms of pig manures and add water to water content 70%, PH to 7.0 is adjusted, mounted in resistant to elevated temperatures modeling
In pocket, 121 DEG C sterilize 30 minutes.
3) above-mentioned zymocyte liquid is pressed 2:1:In proportion 1 after 1 mixing:In the pig manure culture medium that 10 (v/w) accesses are handled well,
Moisture 60% is kept, is rised in value 2-3 days under the conditions of 30 DEG C, the plant-growth promoting rhizobacteria improvement microbial inoculum for expanding culture is produced.
In the present invention, the ratios of Mixed Microbes, inoculum concentration, pig manure moisture content in medium etc. are not limited to the group that embodiment is lifted
Close, different combinations can be selected according to actual conditions.
The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention, all thinkings in the present invention
Within principle, any modifications, equivalent substitutions and improvements done etc. should be included within the scope of the present invention.
Claims (4)
1. a kind of prepare the technology that rare-earth tailing improves microbial inoculum by raw material culture plant-growth promoting rhizobacteria of pig manure, it is characterized in agriculture
Industry waste pig manure is raw material culture plant-growth promoting rhizobacteria, is stored in using on June 24th, 2013 in China typical culture collection
The deposit number of the heart is：CCTCC M 2013283 Bacillus amyloliquefaciens H47 solution starch gemma bar
Bacteria strain, commercially available bacillus licheniformis, commercially available bacillus pumilus are plant growth-promoting microbial inoculum, and preparation prepares rare-earth tailing improvement bacterium
Agent, its technique is：
1) Bacillus amyloliquefaciens H47 by deposit number for CCTCC M 2013283, commercially available lichens bud
The actication of culture such as spore bacillus, commercially available bacillus pumilus are simultaneously prepared into plant growth-promoting microbial inoculum：
(1) take a ring to be rule on PDA plate respectively in three kinds of strain inclined planes and connect mycelia, 30 DEG C of cultures 20 are small in incubator
When；
(2) three kinds of strain single bacterium colonies of picking are respectively connected in the triangular flask equipped with LB fluid nutrient mediums on PDA plate, if preparing
Dry part, shaking table culture, condition of culture：Rotating speed 200r/min, 30 DEG C of temperature incubated 36 hours, that is, obtains plant growth-promoting bacteria
Agent expands the seed of culture.
2) pig manure is pre-processed：Pig manure adds water to water content 40-60%, adjusts PH to 7.0, in resistant to elevated temperatures polybag,
121 DEG C sterilize 30 minutes.
3) in proportion 1 after above-mentioned zymocyte liquid is mixed:In the pig manure culture medium that 10 (v/w) accesses are handled well, moisture is kept
40-60%, rise in value 2-3 days under the conditions of 25-30 DEG C, produce the plant-growth promoting rhizobacteria improvement microbial inoculum for expanding culture.
2. a kind of rare-earth tailing that prepared by raw material culture plant-growth promoting rhizobacteria of pig manure improves microbial inoculum according to claim 1
Technology, it is characterised in that：Plant growth-promoting bacteria deposit number is：CCTCC M 2013283.
3. a kind of rare-earth tailing that prepared by raw material culture plant-growth promoting rhizobacteria of pig manure improves microbial inoculum according to claim 1
Technology, it is characterised in that：Using agricultural wastes pig manure as raw material culture plant-growth promoting rhizobacteria.
4. a kind of rare-earth tailing that prepared by raw material culture plant-growth promoting rhizobacteria of pig manure improves microbial inoculum according to claim 1
Technology, it is characterised in that：The plant growth-promoting microbial inoculum is bacillus amyloliquefaciens, commercially available bacillus licheniformis, commercially available short and small gemma
The mix bacterium agent of bacillus composition.
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| CN201710243533.6A CN106947717A (en) | 2017-04-14 | 2017-04-14 | It is a kind of that the technology that rare-earth tailing improves microbial inoculum is prepared by raw material culture plant-growth promoting rhizobacteria of pig manure |
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| CN201710243533.6A CN106947717A (en) | 2017-04-14 | 2017-04-14 | It is a kind of that the technology that rare-earth tailing improves microbial inoculum is prepared by raw material culture plant-growth promoting rhizobacteria of pig manure |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110423175A (en) * | 2019-08-08 | 2019-11-08 | 中国恩菲工程技术有限公司 | For improveing the modifying agent composition and modifying agent of rare-earth tailing sand |
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| CN104263677A (en) * | 2014-09-02 | 2015-01-07 | 华中农业大学 | Composite microbial seed dressing agent and preparation method and application of composite microbial seed dressing agent |
| CN105149339A (en) * | 2015-03-23 | 2015-12-16 | 江南大学 | Method for preparing ionic rare earth mine tailing in-situ remediation plant nursing system |
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2017
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Patent Citations (3)
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| WO2009128644A2 (en) * | 2008-04-14 | 2009-10-22 | 한국과학기술원 | Genome-scale butanol-producing microorganism metabolic network model and methods using the same for analysis of metabolic characteristics of butanol-producing microorganisms and for screening deletion targets |
| CN104263677A (en) * | 2014-09-02 | 2015-01-07 | 华中农业大学 | Composite microbial seed dressing agent and preparation method and application of composite microbial seed dressing agent |
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| CN110423175A (en) * | 2019-08-08 | 2019-11-08 | 中国恩菲工程技术有限公司 | For improveing the modifying agent composition and modifying agent of rare-earth tailing sand |
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Application publication date: 20170714 |