CN106867948A - It is a kind of to prepare the technology that rare-earth tailing improves microbial inoculum by raw material culture plant-growth promoting rhizobacteria of lactose refining waste water - Google Patents
It is a kind of to prepare the technology that rare-earth tailing improves microbial inoculum by raw material culture plant-growth promoting rhizobacteria of lactose refining waste water Download PDFInfo
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- CN106867948A CN106867948A CN201710243550.XA CN201710243550A CN106867948A CN 106867948 A CN106867948 A CN 106867948A CN 201710243550 A CN201710243550 A CN 201710243550A CN 106867948 A CN106867948 A CN 106867948A
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Abstract
The invention provides a kind of the technology that rare-earth tailing improves microbial inoculum is prepared by raw material culture plant-growth promoting rhizobacteria of lactose refining waste water, be characterized in that with lactose refining waste water be raw material culture plant-growth promoting rhizobacteria, use on June 24th, 2013 be stored in the deposit number of China typical culture collection center for：The Bacillus amyloliquefaciens strain of the Bacillus amyloliquefaciens H47 of CCTCC M 2013283, commercially available bacillus licheniformis, commercially available bacillus pumilus are plant growth-promoting microbial inoculum, and preparation prepares rare-earth tailing improvement microbial inoculum.Rare-earth tailing improvement microbial inoculum is prepared by raw material culture plant-growth promoting rhizobacteria of lactose refining waste water, soil fertility is increased using plant growth-promoting fungi degradation ammonium, be alternatively arranged as a kind of water-loss reducer, promote soil granular structure to be formed, kept soil from packing together；Destruction soil capillarity, prevents soil water evaporation.It is raw material culture plant-growth promoting rhizobacteria to use lactose refining waste water, and wide material sources are cheap, and strain growth rate is fast, and is alternatively arranged as fertilizer, for plant provides various nutriments.Process is simple, low production cost, it is easy to promote.So that lactose refining waste water is rationally utilized, turn waste into wealth.
Description
Technical field
Technology the present invention relates to repair ion type rareearth mine tailing, specifically one kind is with lactose refining waste water as raw material
Culture plant-growth promoting rhizobacteria prepares the technology that rare-earth tailing improves microbial inoculum.Belong to biological technical field.
Background technology
The rare earth element concentration in ion type rareearth mineral deposit is relatively low, and usually in tens to hundreds of ppm, this causes that it is difficult
Efficiently separate.According to relevant report, (holed at the top of Rare-earth Mine, then with sodium chloride, ammonium sulfate using leaching technologies in situ
Injected from the hilltop Deng electrolyte solution), 1t rare earth oxides are obtained from ion type rareearth mineral deposit, it is necessary to 300m2Face plant
It is cost, and it has 2000t tailings dischargings to neighbouring mountain valley or river.The exploitation of rare earth resources causes large area vegetation to break
It is bad, easily trigger soil erosion, the destruction to ecological environment is very important, meanwhile, dump leaching, leaching in situ that Rare Earth Mine exploitation is used
The method such as taking can make the activity disappearance of soil, be unfavorable for plant growth.Therefore, the efficient resource utilization of rare-earth tailing is to ecology
The importance of environment is self-evident.
The deposit number that this patent is related to is：The Bacillus amyloliquefaciens of CCTCC M 2013283
The bacillus amyloliquefaciens of H47 have good plant growth-promoting characteristic.Rhizosphere plant-growth promoting rhizobacteria can be colonized in root system of plant,
And the bacterium favourable to growth and development of plants.Commercially available bacillus licheniformis and commercially available bacillus pumilus and Bacillus
Amyloliquefaciens H47 composition mix bacterium agents can assist Bacillus amyloliquefaciens H47 to promote plant
Growth.The cost of material of culture plant-growth promoting rhizobacteria is high at present, is unfavorable for the large-scale application of plant growth-promoting microbial inoculum.Therefore grind
Studying carefully and set up the technology for preparing rare-earth tailing improvement microbial inoculum as raw material culture plant-growth promoting rhizobacteria with lactose refining waste water has important
Meaning.
Lactose waste water be the waste water produced during lactose refining from Zhenjiang Kang Fu bioengineering Co., Ltd, its pH exists
3.8 or so, protein content 1.2%, the content of lactose is about 3%.If be directly discharged in environment, cause environment dirty
Dye.
The content of the invention
The present invention prepares the technology that rare-earth tailing improves microbial inoculum by raw material culture plant-growth promoting rhizobacteria of lactose refining waste water.
It is with deposit number：The bacillus amyloliquefaciens of the Bacillus amyloliquefaciens H47 of CCTCC M 2013283,
Commercially available bacillus licheniformis and commercially available bacillus pumilus are plant growth-promoting microbial inoculum, are added after to lactose refining Wastewater Pretreatment
Enter plant growth-promoting bacteria and be enlarged culture, that is, obtain a large amount of ionics for having and improving soil physical chemistry situation, promote plant growth
The plant growth-promoting microbial inoculum of rare-earth tailing in-situ immobilization.The method has obvious promotion to make plant in the growth of ionic mine tailing
With.Lactose refining waste water is rationally utilized, and is turned waste into wealth, cheap and simple and easy to do.
Technical scheme：
The deposit number for being stored in China typical culture collection center with June 24th, 2013 is：CCTCC M
The bacillus amyloliquefaciens of 2013283 Bacillus amyloliquefaciens H47, commercially available bacillus licheniformis is commercially available
Bacillus pumilus mixed plant growth promoting bacteria agent, is raw material culture plant-growth promoting rhizobacteria with lactose refining waste water, prepares rare earth tail
Ore deposit improves the technology of microbial inoculum.Technique is：
1) it is the Bacillus amyloliquefaciens H47, commercially available of CCTCC M 2013283 by deposit number
The actication of culture such as clothing bacillus, commercially available bacillus pumilus are simultaneously prepared into plant growth-promoting microbial inoculum：
(1) ring is taken respectively rule on PDA plate connect mycelia in three kinds of strain inclined planes, 30 DEG C of cultures 20 in incubator
Hour；
(2) three kinds of strain single bacterium colonies of picking are respectively connected in the triangular flask equipped with LB fluid nutrient mediums on PDA plate, accurate
Standby several pieces, shaking table culture, condition of culture：Rotating speed 200r/min, 30 DEG C of temperature incubated 20 hours, that is, obtains plant rush
The seed of bacteria-promoting agent Amplification Culture.
2) lactose refining Wastewater Pretreatment：Regulation lactose refining waste water water content adjusts PH to 7.0, dress to 60-75%
In resistant to elevated temperatures polybag, 121 DEG C sterilize 30 minutes.
3) in proportion 1 after above-mentioned zymocyte liquid is mixed:10 (v/w) access the lactose refining wastewater medium handled well
In, moisture 50-70% is kept, rise in value 2-3 days under the conditions of 25-30 DEG C, obtain final product the plant-growth promoting rhizobacteria improvement of Amplification Culture
Microbial inoculum.
Beneficial effects of the present invention：
1) present invention improves microbial inoculum with the rare-earth tailing that lactose refining waste water is prepared as raw material culture plant-growth promoting rhizobacteria, can
Increase soil fertility with using plant growth-promoting fungi degradation ammonium.A kind of water-loss reducer is alternatively arranged as, promotes soil granular structure to be formed, prevented
Soil hardening；Destruction soil capillarity, prevents soil water evaporation.
2) present invention lactose refining waste water is raw material culture plant-growth promoting rhizobacteria, and wide material sources are cheap, and strain increases
Grow speed fast, and be alternatively arranged as fertilizer, for plant provides various nutriments.
3) rationally utilized the invention enables lactose refining waste water, turned waste into wealth.
4) process is simple of the invention, low production cost, it is easy to promote.
Specific embodiment
Below in conjunction with the embodiment of the present invention, technical scheme is carried out to describe clear, perfectly, it is clear that institute
The embodiment of description is only a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention,
The other embodiment that those of ordinary skill in the art are obtained on the premise of creative work is not made, belongs to the present invention
The scope of protection.
Embodiment 1
1) it is the Bacillus amyloliquefaciens H47, commercially available of CCTCC M 2013283 by deposit number
The actication of culture such as clothing bacillus, commercially available bacillus pumilus are simultaneously prepared into plant growth-promoting microbial inoculum：
(1) ring is taken respectively rule on PDA plate connect mycelia in three kinds of strain inclined planes, 30 DEG C of cultures 20 in incubator
Hour；
(2) three kinds of strain single bacterium colonies of picking are respectively connected to the 250ml equipped with 50ml LB liquid mediums on PDA plate
In triangular flask, each bacterium is inoculated with 12, shaking table culture, condition of culture：Rotating speed 175r/min, 30 DEG C of temperature, incubated 35 is small
When, that is, obtain the seed of plant growth-promoting bacteria agent Amplification Culture.
2) lactose refining Wastewater Pretreatment：Weigh 3 kg lactose refined wastewaters and adjust water content to 60%, regulation PH to
7.0, in resistant to elevated temperatures polybag, 121 DEG C sterilize 30 minutes.
3) above-mentioned zymocyte liquid is pressed 1:1:In proportion 1 after 1 mixing:10 (v/w) access the lactose refining waste water handled well
In culture medium, moisture 50% is kept, rised in value 2-3 days under the conditions of 30 DEG C, obtain final product the plant-growth promoting rhizobacteria improvement bacterium of Amplification Culture
Agent.
Embodiment 2
1) it is the Bacillus amyloliquefaciens H47, commercially available of CCTCC M 2013283 by deposit number
The actication of culture such as clothing bacillus, commercially available bacillus pumilus are simultaneously prepared into plant growth-promoting microbial inoculum：
(1) ring is taken respectively rule on PDA plate connect mycelia in three kinds of strain inclined planes, 30 DEG C of cultures 18 in incubator
Hour；
(2) three kinds of strain single bacterium colonies of picking are respectively connected to the 250ml equipped with 50ml LB liquid mediums on PDA plate
In triangular flask, each bacterium is inoculated with 12, shaking table culture, condition of culture：Rotating speed 220r/min, 32 DEG C of temperature, incubated 28 is small
When, that is, obtain the seed of plant growth-promoting bacteria agent Amplification Culture.
2) lactose refining Wastewater Pretreatment：Weigh 3 kg lactose refined wastewaters and adjust water content to 70%, regulation PH to
7.0, in resistant to elevated temperatures polybag, 121 DEG C sterilize 30 minutes.
3) above-mentioned zymocyte liquid is pressed 1:2:In proportion 1 after 1 mixing:10 (v/w) access the lactose refining waste water handled well
In culture medium, moisture 60% is kept, rised in value 2-3 days under the conditions of 30 DEG C, obtain final product the plant-growth promoting rhizobacteria improvement bacterium of Amplification Culture
Agent.
Embodiment 3
1) it is the Bacillus amyloliquefaciens H47, commercially available of CCTCC M 2013283 by deposit number
The actication of culture such as clothing bacillus, commercially available bacillus pumilus are simultaneously prepared into plant growth-promoting microbial inoculum：
(1) ring is taken respectively rule on PDA plate connect mycelia in three kinds of strain inclined planes, 30 DEG C of cultures 22 in incubator
Hour；
(2) three kinds of strain single bacterium colonies of picking are respectively connected to the 250ml equipped with 50ml LB liquid mediums on PDA plate
In triangular flask, each bacterium is inoculated with 12, shaking table culture, condition of culture：Rotating speed 200r/min, 26 DEG C of temperature, incubated 30 is small
When, that is, obtain the seed of plant growth-promoting bacteria agent Amplification Culture.
2) lactose refining Wastewater Pretreatment：3 kg lactose refined wastewaters regulation water content 65% is weighed, PH is to 7.0 for regulation,
In resistant to elevated temperatures polybag, 121 DEG C sterilize 30 minutes.
3) above-mentioned zymocyte liquid is pressed 1:1:In proportion 1 after 2 mixing:10 (v/w) access the lactose refining waste water handled well
In culture medium, moisture 55% is kept, rised in value 2-3 days under the conditions of 30 DEG C, obtain final product the plant-growth promoting rhizobacteria improvement bacterium of Amplification Culture
Agent.
Embodiment 4
1) it is the Bacillus amyloliquefaciens H47, commercially available of CCTCC M 2013283 by deposit number
The actication of culture such as clothing bacillus, commercially available bacillus pumilus are simultaneously prepared into plant growth-promoting microbial inoculum：
(1) ring is taken respectively rule on PDA plate connect mycelia in three kinds of strain inclined planes, 30 DEG C of cultures 21 in incubator
Hour；
(2) three kinds of strain single bacterium colonies of picking are respectively connected to the 250ml equipped with 50ml LB liquid mediums on PDA plate
In triangular flask, each bacterium is inoculated with 12, shaking table culture, condition of culture：Rotating speed 180r/min, 26 DEG C of temperature, incubated 30 is small
When, that is, obtain the seed of plant growth-promoting bacteria agent Amplification Culture.
2) lactose refining Wastewater Pretreatment：Weigh 3 kg lactose refined wastewaters and adjust water content to 60%, regulation PH to
7.0, in resistant to elevated temperatures polybag, 121 DEG C sterilize 30 minutes.
3) above-mentioned zymocyte liquid is pressed 2:1:In proportion 1 after 1 mixing:10 (v/w) access the lactose refining waste water handled well
In culture medium, moisture 50% is kept, rised in value 2-3 days under the conditions of 30 DEG C, obtain final product the plant-growth promoting rhizobacteria improvement bacterium of Amplification Culture
Agent.
In the present invention, ratio, inoculum concentration, lactose refining wastewater medium water content of Mixed Microbes etc. are not limited to embodiment
The combination lifted, can select different combinations according to actual conditions.
Preferred embodiments of the invention are the foregoing is only, is not intended to limit the invention, it is all in thinking of the invention
Within principle, any modification, equivalent substitution and improvements done etc. should be included within the scope of the present invention.
Claims (4)
1. a kind of to prepare the technology that rare-earth tailing improves microbial inoculum by raw material culture plant-growth promoting rhizobacteria of lactose refining waste water, it is special
Point is, with lactose refining waste water as raw material culture plant-growth promoting rhizobacteria, Chinese Typical Representative culture to be stored in using on June 24th, 2013
The deposit number of thing collection is：The Xie Dian of the Bacillus amyloliquefaciens H47 of CCTCC M 2013283
Afnyloliquefaciens bacterial strain, commercially available bacillus licheniformis, commercially available bacillus pumilus are plant growth-promoting microbial inoculum, and preparation prepares rare earth tail
Ore deposit improves microbial inoculum, and its technique is：
1) it is Bacillus amyloliquefaciens H47, the commercially available lichens bud of CCTCC M 2013283 by deposit number
The actication of culture such as spore bacillus, commercially available bacillus pumilus are simultaneously prepared into plant growth-promoting microbial inoculum：
(1) ring is taken respectively being rule on PDA plate and connect mycelia, 30 DEG C of cultures 20 are small in incubator in three kinds of strain inclined planes
When；
(2) three kinds of strain single bacterium colonies of picking are respectively connected in the triangular flask equipped with LB fluid nutrient mediums on PDA plate, if preparing
Dry part, shaking table culture, condition of culture：Rotating speed 200r/min, 30 DEG C of temperature incubated 20 hours, that is, obtains plant growth-promoting bacteria
The seed of agent Amplification Culture.
2) lactose refining Wastewater Pretreatment：Regulation lactose refining waste water water content adjusts PH to 7.0, mounted in resistance to 60-75%
In the polybag of high temperature, 121 DEG C sterilize 30 minutes.
3) in proportion 1 after above-mentioned zymocyte liquid is mixed:10 (v/w) are accessed in the lactose refining wastewater medium handled well, are protected
Water holding point 50-70%, rises in value 2-3 days under the conditions of 25-30 DEG C, obtains final product the plant-growth promoting rhizobacteria improvement microbial inoculum of Amplification Culture.
2. it is a kind of according to claim 1 rare-earth tailing is prepared as raw material culture plant-growth promoting rhizobacteria with lactose refining waste water to change
The technology of good microbial inoculum, it is characterised in that：Plant growth-promoting bacteria deposit number is：CCTCC M 2013283.
3. it is a kind of according to claim 1 rare-earth tailing is prepared as raw material culture plant-growth promoting rhizobacteria with lactose refining waste water to change
The technology of good microbial inoculum, it is characterised in that：It is raw material culture plant-growth promoting rhizobacteria with lactose refining waste water.
4. it is a kind of according to claim 1 rare-earth tailing is prepared as raw material culture plant-growth promoting rhizobacteria with lactose refining waste water to change
The technology of good microbial inoculum, it is characterised in that：The plant growth-promoting microbial inoculum is bacillus amyloliquefaciens, commercially available bacillus licheniformis, commercially available
The mix bacterium agent of bacillus pumilus composition.
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| CN201710243550.XA CN106867948A (en) | 2017-04-14 | 2017-04-14 | It is a kind of to prepare the technology that rare-earth tailing improves microbial inoculum by raw material culture plant-growth promoting rhizobacteria of lactose refining waste water |
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| CN201710243550.XA CN106867948A (en) | 2017-04-14 | 2017-04-14 | It is a kind of to prepare the technology that rare-earth tailing improves microbial inoculum by raw material culture plant-growth promoting rhizobacteria of lactose refining waste water |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112877252A (en) * | 2021-03-09 | 2021-06-01 | 中南大学 | Composite microbial agent for leaching weathering crust leaching type rare earth ore and preparation method thereof |
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|---|---|---|---|---|
| CN103820349A (en) * | 2012-11-16 | 2014-05-28 | 中国科学院生态环境研究中心 | Bacillus licheniformis and preparation method and application of fungicide |
| CN104663726A (en) * | 2014-11-14 | 2015-06-03 | 江南大学 | Method for preparing bactericide for preventing and treating plant blight by fermenting lactose refined wastewater |
| CN105149339A (en) * | 2015-03-23 | 2015-12-16 | 江南大学 | Method for preparing ionic rare earth mine tailing in-situ remediation plant nursing system |
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2017
- 2017-04-14 CN CN201710243550.XA patent/CN106867948A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103820349A (en) * | 2012-11-16 | 2014-05-28 | 中国科学院生态环境研究中心 | Bacillus licheniformis and preparation method and application of fungicide |
| CN104663726A (en) * | 2014-11-14 | 2015-06-03 | 江南大学 | Method for preparing bactericide for preventing and treating plant blight by fermenting lactose refined wastewater |
| CN105149339A (en) * | 2015-03-23 | 2015-12-16 | 江南大学 | Method for preparing ionic rare earth mine tailing in-situ remediation plant nursing system |
Non-Patent Citations (1)
| Title |
|---|
| 杜春梅: "《芽孢杆菌在农业中的研究与应用》", 31 May 2013, 黑龙江大学出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112877252A (en) * | 2021-03-09 | 2021-06-01 | 中南大学 | Composite microbial agent for leaching weathering crust leaching type rare earth ore and preparation method thereof |
| CN112877252B (en) * | 2021-03-09 | 2022-05-10 | 中南大学 | Composite microbial agent for leaching weathering crust leaching type rare earth ore and preparation method thereof |
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Application publication date: 20170620 |