CN104925957A - Composite water restoration agent containing bacillus - Google Patents
Composite water restoration agent containing bacillus Download PDFInfo
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- CN104925957A CN104925957A CN201510104650.5A CN201510104650A CN104925957A CN 104925957 A CN104925957 A CN 104925957A CN 201510104650 A CN201510104650 A CN 201510104650A CN 104925957 A CN104925957 A CN 104925957A
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Abstract
The invention relates to a composite water restoration agent containing bacillus. The restoration agent comprises the components of, by weight, 1-25 parts of bacillus subtilis, 1-25 parts of bacillus licheniformis, 1-25 parts of bacillus amyloliquefaciens, 1-25 parts of bacillus pumilus, 10-30 parts of sodium hydrogen carbonate, 10-30 parts of sodium percarbonate, 10-30 parts of calcium carbonate, 5-20 parts of citric acid, and 5-20 parts of polymerized aluminum sulfate. The water restoration agent has the following advantages: the four bacillus medicinal viable cell powders and the compounds such as an oxygenating agent are combined according to a certain ratio, such that the four active products and the compounds provide a synergistic effect; with the composite water restoration agent, the cost is low, and production process is simple; with the water restoration agent, culture water COD, nitrite value and ammonia nitrogen value can be effectively reduced, and a significant restoration function is provided for a polluted water body.
Description
Technical field
The invention belongs to field of environment protection, be specifically related to a kind of compound water body renovation agent containing genus bacillus.
Background technology
Water is that human society is depended on for existence the most indispensable natural resources, is also the basic resource of social sustainable development.In recent years, along with the accelerated development of industry and the thin of people's environmental consciousness, exacerbate the transition consumption of world water resources, and Different Waters is polluted in various degree, become the Economic development of restriction China and the bottleneck affecting national health.Therefore, the problem solving Freshwater resources sustainable use is very urgent.Microbial environment repairing method is a kind of green environment administering method developed this year, has repair time short, easy and simple to handle, the features such as expense is low, environmental friendliness.Genus bacillus is a kind of conventional probiotic bacterium utilizing microorganism remediation water body environment, mainly comprise bacillus amyloliquefaciens, the type bacterial strain has improvement water quality, has obviously clear up effect to ammonia nitrogen in aquaculture water and bed mud, nitric nitrogen, nitrite nitrogen, COD equal size; Bacillus pumilus, the type bacterial strain has stronger extracellular protease, lipase, amylase activity, has inhibition widely, and do not have hemolytic activity to vibrios, is a kind of microbial preparation preventing and treating aquatic products bacterial disease; Bacillus licheniformis, the type bacterial strain has biological flocculant, the High Cellulase Production of throwing out, to Cellulose precipitates, there is splendid degradation effect, multiple enzyme can be secreted, the ammonia nitrogen in degradable aquaculture water, remaining dietary protein and starch, purifying aquatic water simultaneously.
Compound water body renovation agent is that a class contains microbial components and the composite novel water body renovation agent of compound component, and carry out the reparation of globality from the biological many-side of physical chemistry, three kinds of reparations are complemented each other, and mutually promotes and reaches the object of water remediation.Wherein bioremediation technology has the advantage of environmental friendliness, ecological energy-saving, is main body recovery technique most with prospects.
Summary of the invention
Object of the present invention just in order to overcome the deficiency of above-mentioned technology, and provides a kind of compound water body renovation agent containing genus bacillus, is a kind of compound water body renovation agent containing genus bacillus and compound.
The object of the invention is to have come by following technical solution.This compound water body renovation agent containing genus bacillus, this renovation agent formula is composed as follows: subtilis 1 ~ 25 part, Bacillus licheniformis 1 ~ 25 part, bacillus amyloliquefaciens 1 ~ 25 part, bacillus pumilus 1 ~ 25 part, sodium bicarbonate 10 ~ 30 parts, SPC-D 10 ~ 30 parts, percarbonic acid calcium 10 ~ 30 parts, Citric Acid 5 ~ 20 parts, polyaluminium sulfate 5 ~ 20 parts.
As preferably, described renovation agent formula is composed as follows: subtilis 7.5 parts, Bacillus licheniformis 7.5 parts, bacillus amyloliquefaciens 7.5 parts, bacillus pumilus 7.5 parts, sodium bicarbonate 20 parts, SPC-D 20 parts, percarbonic acid calcium 10 parts, Citric Acid 15 parts, polyaluminium sulfate 15 parts.
As preferably, described renovation agent formula is composed as follows: subtilis 25 parts, Bacillus licheniformis 25 parts, bacillus amyloliquefaciens 25 parts, bacillus pumilus 25 parts, sodium bicarbonate 20 parts, SPC-D 20 parts, percarbonic acid calcium 20 parts, Citric Acid 20 parts, polyaluminium sulfate 20 parts.
As preferably, described renovation agent formula is composed as follows: subtilis 10 parts, Bacillus licheniformis 10 parts, bacillus amyloliquefaciens 10 parts, bacillus pumilus 10 parts, sodium bicarbonate 10 parts, SPC-D 15 parts, percarbonic acid calcium 15 parts, Citric Acid 10 parts, polyaluminium sulfate 20 parts.
As preferably, described renovation agent formula is composed as follows: subtilis 12.5 parts, Bacillus licheniformis 12.5 parts, bacillus amyloliquefaciens 12.5 parts, bacillus pumilus 12.5 parts, sodium bicarbonate 5 parts, SPC-D 10 parts, percarbonic acid calcium 10 parts, Citric Acid 5 parts, polyaluminium sulfate 20 parts.
Beneficial effect of the present invention is: the compounds such as four kinds of medicinal viable bacteria powder of genus bacillus and oxygenation agent combine by a certain percentage, plants the collaborative onset of active result and compound; Adopt compound water body of the present invention, with low cost, production technique is simple, and effectively can reduce the COD in aquaculture water, nitrous values of nitrogen might and ammonia nitrogen value, have polluted-water and obviously repair effect.
Embodiment
Below by embodiment, the present invention is further elaborated, and help is understood the present invention by embodiment better, but the present invention is not limited only to following embodiment.
This compound water body renovation agent containing genus bacillus of the present invention, this renovation agent formula is composed as follows: subtilis 1 ~ 25 part, Bacillus licheniformis 1 ~ 25 part, bacillus amyloliquefaciens 1 ~ 25 part, bacillus pumilus 1 ~ 25 part, sodium bicarbonate 10 ~ 30 parts, SPC-D 10 ~ 30 parts, percarbonic acid calcium 10 ~ 30 parts, Citric Acid 5 ~ 20 parts, polyaluminium sulfate 5 ~ 20 parts, by each component by above ratio combination, after mixing, compression molding is compound water body renovation agent.
The use of compound water body renovation agent: be fed in shrimp culture pond by compound for 1 ~ 5kg renovation agent, measures COD every certain hour water sampling, oxygen dissolving value, nitrous values of nitrogen might, ammonia nitrogen value.Method common experimental conditions of the present invention is just easily accomplished, simple to operate, and produce short around, output is high, has wide purposes.
Wherein said bacterial strain is all in the preservation of Chinese Typical Representative culture culture presevation administrative center, address Wuhan University of Wuhan City of Hubei China province, preservation date on October 15th, 2014, comprise subtilis Bacillus subtilis, preserving number HZW1CCTCC M 2014479; Bacillus licheniformis Bacillus licheniformis, preserving number HZW3CCTCC M 2014480; Bacillus amyloliquefaciens Bacillus amyloliquefaciens, preserving number HZW4CCTCC M 2014481; Bacillus pumilus Bacillus pumilus, preserving number HZW5CCTCC M 201482.
Principle: the compounds such as four kinds of medicinal viable bacteria powder of genus bacillus and oxygenation agent are combined by a certain percentage; In the medicinal viable bacteria powder of genus bacillus containing viable bacteria about 30,000,000,000/gram; Compound comprises oxygenation agent SPC-D, percarbonic acid calcium, sodium bicarbonate, Citric Acid, flocculant poly Tai-Ace S 150.The collaborative onset of various active product and compound, after dropping into sewage, first oxygenation agent continues to produce a large amount of miniature oxygen bubbles.Increase water body oxygen level (DO value), meet growth of aerobic microorganisms breeding necessary factor, a large amount of miniature oxygen bubbless can stir mud simultaneously, and microorganism is fully contacted sludge components and nutritive substance.Product drops into after water body, and sink to basin bottom, tablet just gets started releasing microbe, the organism making it be attached to left floating, oil layer and bottom.The effervescent effect of product self stirs the mud at the bottom of pond, and biological restoration is carried out to water body in each corner that microorganism can be made to be distributed in sewage lagoon rapidly and uniformly.This product obviously can repair aquaculture wastewater, and reduce the suspended substance in water body, BOD, COD, phosphoric acid salt, ammonia-nitrogen content, has wide production application prospect.
Embodiment 1:
1, microbial inoculum: described microbial inoculum formula is composed as follows: subtilis 25 parts, Bacillus licheniformis 25 parts, bacillus amyloliquefaciens 25 parts, bacillus pumilus 25 parts, by each component by above ratio combination, compression molding after mixing.
Adjuvant: described microbial inoculum formula is composed as follows: sodium bicarbonate 20 parts, SPC-D 20 parts, percarbonic acid calcium 20 parts, Citric Acid 20 parts, polyaluminium sulfate 20 parts.By each component by above ratio combination, after mixing, compression molding is compound water body renovation agent.
Compound water body renovation agent preparation: described renovation agent formula is pressed microbial inoculum and mixed rear compression molding with adjuvant 1:1.
2, above three types preparation is dosed in the corresponding 10L ratio of 1g Wastewater Sample that city inland river extracts respectively, measures suspended substance (SS) every certain hour water sampling, five-day BOD (BOD
5), chemical oxygen demand (COD) (COD), total nitrogen (TN), total phosphorus (TP) and pH value.Its experimental result is as shown in the table, and compound water body renovation agent obviously can improve Water quality fast, and comparatively speaking, single adjuvant only has an impact to water body in the early stage, the not zoic long-lasting reparation of tool; And for want of adjuvant repair of early stage to water body of single microbial inoculum, show certain lag phase.Above example shows, only has when adjuvant and microbial inoculum carry out reasonably compositely could arriving the repair fast long-acting again to water body.
The effect of compound water body renovation agent
Index | Before feeding intake | Feed intake 48 hours | Feed intake 96 hours | Feed intake 144 hours |
SS | 208mg/L | 15.12mg/L | 10.31mg/L | 6.19mg/L |
BOD 5 | 65.36mg/L | 32.75mg/L | 23.5mg/L | 7.12mg/L |
CODcr | 156.47mg/L | 36.38mg/L | 16.72mg/L | 12.13mg/L |
TN | 28.11mg/L | 13.22mg/L | 5.15mg/L | 1.05mg/L |
TP | 8.36mg/L | 3.15mg/L | 1.51mg/L | 0.31mg/L |
pH | 7.19 | 7.32 | 7.35 | 7.27 |
Microbial inoculum effect
Index | Before feeding intake | Feed intake 48 hours | Feed intake 96 hours | Feed intake 144 hours |
SS | 210mg/L | 209mg/L | 180mg/L | 133mg/L |
BOD 5 | 68.71mg/L | 60.75mg/L | 30.5mg/L | 11.12mg/L |
CODcr | 150.33mg/L | 136.62mg/L | 66.72mg/L | 15.13mg/L |
TN | 25.81mg/L | 23.22mg/L | 6.73mg/L | 0.75mg/L |
TP | 8.24mg/L | 7.33mg/L | 3.11mg/L | 0.56mg/L |
pH | 7.23 | 7.42 | 7.64 | 7.58 |
Adjuvant effect
Index | Before feeding intake | Feed intake 48 hours | Feed intake 96 hours | Feed intake 144 hours |
[0027]
SS | 201mg/L | 33.13mg/L | 25.19mg/L | 20.83mg/L |
BOD 5 | 70.83mg/L | 60.75mg/L | 50.25mg/L | 47.52mg/L |
CODcr | 140.63mg/L | 76.23mg/L | 68.72mg/L | 65.13mg/L |
TN | 26.65mg/L | 18.22mg/L | 10.15mg/L | 8.78mg/L |
TP | 7.95mg/L | 6.33mg/L | 4.11mg/L | 2.31mg/L |
pH | 7.29 | 7.35 | 7.53 | 7.57 |
Embodiment 2:
1, compound water body renovation agent preparation: described renovation agent formula is composed as follows: subtilis 7.5 parts, Bacillus licheniformis 7.5 parts, bacillus amyloliquefaciens 7.5 parts, bacillus pumilus 7.5 parts, sodium bicarbonate 20 parts, SPC-D 20 parts, percarbonic acid calcium 10 parts, Citric Acid 15 parts, polyaluminium sulfate 15 parts.By each component by above ratio combination, after mixing, compression molding is compound water body renovation agent.
2, the use of compound water body renovation agent: compound for 5kg renovation agent is fed into 5000m
3in shrimp culture pond, test every certain hour water sampling, data are as following table.
Index | Before feeding intake | After feeding intake |
SS | 47.83mg/L | 13.56mg/L |
BOD 5 | 23.93mg/L | 5.32mg/L |
CODcr | 39.51mg/L | 10.52mg/L |
TN | 6.72mg/L | 0.55mg/L |
TP | 2.75mg/L | 0.25mg/L |
pH | 7.37 | 7.38 |
Embodiment 3:
1, compound water body renovation agent preparation: described renovation agent formula is composed as follows: subtilis 12.5 parts, Bacillus licheniformis 12.5 parts, bacillus amyloliquefaciens 12.5 parts, bacillus pumilus 12.5 parts, sodium bicarbonate 5 parts, SPC-D 10 parts, percarbonic acid calcium 10 parts, Citric Acid 5 parts, polyaluminium sulfate 20 parts.By each component by above ratio combination, after mixing, compression molding is compound water body renovation agent.
2, the use of compound water body renovation agent: compound renovation agent is fed into 500m by the charging capacity of 5kg/ days
3wastewater from Pig Farm collecting tank, throws in 10 days continuously.Test data before and after throwing in is as following table.
Index | Before feeding intake | After feeding intake |
SS | 2300mg/L | 190mg/L |
[0036]
BOD 5 | 1300mg/L | 120mg/L |
CODcr | 2700mg/L | 300mg/L |
TN | 780mg/L | 10.95mg/L |
TP | 35mg/L | 0.88mg/L |
pH | 7.65 | 7.31 |
Embodiment 4:
1, compound water body renovation agent preparation: described renovation agent formula is composed as follows: subtilis 10 parts, Bacillus licheniformis 10 parts, bacillus amyloliquefaciens 10 parts, bacillus pumilus 10 parts, sodium bicarbonate 10 parts, SPC-D 15 parts, percarbonic acid calcium 15 parts, Citric Acid 10 parts, polyaluminium sulfate 20 parts.By each component by above ratio combination, after mixing, compression molding is compound water body renovation agent.
2, the use of compound water body renovation agent: compound renovation agent is fed into 1000m by the charging capacity of 1kg/ days
3municipal wastewater collecting tank, throws in 10 days continuously.Test data before and after throwing in is as following table.
Index | Before feeding intake | After feeding intake |
SS | 580mg/L | 10.82mg/L |
BOD 5 | 130mg/L | 12.53mg/L |
CODcr | 380mg/L | 25.2mg/L |
TN | 48.11mg/L | 2.95mg/L |
TP | 9.35mg/L | 0.35mg/L |
pH | 6.87 | 7.23 |
In addition to the implementation, the present invention can also have other embodiments.All employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop on the protection domain of application claims.
Claims (5)
1. the compound water body renovation agent containing genus bacillus, it is characterized in that: this renovation agent formula is composed as follows: subtilis 1 ~ 25 part, Bacillus licheniformis 1 ~ 25 part, bacillus amyloliquefaciens 1 ~ 25 part, bacillus pumilus 1 ~ 25 part, sodium bicarbonate 10 ~ 30 parts, SPC-D 10 ~ 30 parts, percarbonic acid calcium 10 ~ 30 parts, Citric Acid 5 ~ 20 parts, polyaluminium sulfate 5 ~ 20 parts.
2. the compound water body renovation agent containing genus bacillus according to claim 1, it is characterized in that: described renovation agent formula is composed as follows: subtilis 7.5 parts, Bacillus licheniformis 7.5 parts, bacillus amyloliquefaciens 7.5 parts, bacillus pumilus 7.5 parts, sodium bicarbonate 20 parts, SPC-D 20 parts, percarbonic acid calcium 10 parts, Citric Acid 15 parts, polyaluminium sulfate 15 parts.
3. the compound water body renovation agent containing genus bacillus according to claim 1, it is characterized in that: described renovation agent formula is composed as follows: subtilis 25 parts, Bacillus licheniformis 25 parts, bacillus amyloliquefaciens 25 parts, bacillus pumilus 25 parts, sodium bicarbonate 20 parts, SPC-D 20 parts, percarbonic acid calcium 20 parts, Citric Acid 20 parts, polyaluminium sulfate 20 parts.
4. the compound water body renovation agent containing genus bacillus according to claim 1, it is characterized in that: described renovation agent formula is composed as follows: subtilis 10 parts, Bacillus licheniformis 10 parts, bacillus amyloliquefaciens 10 parts, bacillus pumilus 10 parts, sodium bicarbonate 10 parts, SPC-D 15 parts, percarbonic acid calcium 15 parts, Citric Acid 10 parts, polyaluminium sulfate 20 parts.
5. the compound water body renovation agent containing genus bacillus according to claim 1, it is characterized in that: described renovation agent formula is composed as follows: subtilis 12.5 parts, Bacillus licheniformis 12.5 parts, bacillus amyloliquefaciens 12.5 parts, bacillus pumilus 12.5 parts, sodium bicarbonate 5 parts, SPC-D 10 parts, percarbonic acid calcium 10 parts, Citric Acid 5 parts, polyaluminium sulfate 20 parts.
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Cited By (11)
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CN105855287A (en) * | 2016-05-30 | 2016-08-17 | 湖南恒凯环保科技投资有限公司 | Method for ex-situ remediation of organic contaminated soil by virtue of sodium percarbonate-biological pile technique |
CN106277354A (en) * | 2016-08-26 | 2017-01-04 | 夏百庆 | A kind of duplicate removal metal water quality renovation agent |
CN106947718A (en) * | 2017-04-14 | 2017-07-14 | 江南大学 | It is a kind of that the technology that rare-earth tailing improves microbial inoculum is prepared by raw material culture plant-growth promoting rhizobacteria of piggery wastewater |
CN106987535A (en) * | 2017-03-01 | 2017-07-28 | 沃邦环保有限公司 | It is a kind of for complex micro organism fungicide of river regulation and preparation method thereof, river sewage processing method |
CN106987536A (en) * | 2017-03-01 | 2017-07-28 | 沃邦环保有限公司 | Complex micro organism fungicide for river regulation and its preparation method and application |
CN108148791A (en) * | 2018-03-07 | 2018-06-12 | 天津科技大学 | A kind of probiotics preparation for improving water quality in aquaculture and preparation method thereof |
CN108504593A (en) * | 2018-03-27 | 2018-09-07 | 丽水学院 | A kind of compound microbial preparation, preparation method and its application in sewage disposal |
CN108517304A (en) * | 2018-03-27 | 2018-09-11 | 丽水学院 | A kind of composite microbial Tetramune, preparation method and its application in sewage disposal |
CN108546662A (en) * | 2018-05-07 | 2018-09-18 | 中国海洋大学 | Using the method for nitrifying bacteria community-bacillus Combined Treatment breeding wastewater of immobilization respectively |
CN109650556A (en) * | 2019-01-23 | 2019-04-19 | 武汉丰甜生物科技有限公司 | The composite bacillus microbial inoculum and its application of ammonia nitrogen and nitrite in degradation water body |
CN111100830A (en) * | 2020-03-09 | 2020-05-05 | 闽江学院 | Composite microecological preparation for aquaculture water purification and application thereof |
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CN106277354A (en) * | 2016-08-26 | 2017-01-04 | 夏百庆 | A kind of duplicate removal metal water quality renovation agent |
CN106987535A (en) * | 2017-03-01 | 2017-07-28 | 沃邦环保有限公司 | It is a kind of for complex micro organism fungicide of river regulation and preparation method thereof, river sewage processing method |
CN106987536A (en) * | 2017-03-01 | 2017-07-28 | 沃邦环保有限公司 | Complex micro organism fungicide for river regulation and its preparation method and application |
CN106947718A (en) * | 2017-04-14 | 2017-07-14 | 江南大学 | It is a kind of that the technology that rare-earth tailing improves microbial inoculum is prepared by raw material culture plant-growth promoting rhizobacteria of piggery wastewater |
CN108148791A (en) * | 2018-03-07 | 2018-06-12 | 天津科技大学 | A kind of probiotics preparation for improving water quality in aquaculture and preparation method thereof |
CN108504593A (en) * | 2018-03-27 | 2018-09-07 | 丽水学院 | A kind of compound microbial preparation, preparation method and its application in sewage disposal |
CN108517304A (en) * | 2018-03-27 | 2018-09-11 | 丽水学院 | A kind of composite microbial Tetramune, preparation method and its application in sewage disposal |
CN108546662A (en) * | 2018-05-07 | 2018-09-18 | 中国海洋大学 | Using the method for nitrifying bacteria community-bacillus Combined Treatment breeding wastewater of immobilization respectively |
CN108546662B (en) * | 2018-05-07 | 2021-01-29 | 中国海洋大学 | Method for jointly treating aquaculture wastewater by respectively immobilized nitrifying bacteria group-bacillus |
CN109650556A (en) * | 2019-01-23 | 2019-04-19 | 武汉丰甜生物科技有限公司 | The composite bacillus microbial inoculum and its application of ammonia nitrogen and nitrite in degradation water body |
CN111100830A (en) * | 2020-03-09 | 2020-05-05 | 闽江学院 | Composite microecological preparation for aquaculture water purification and application thereof |
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