A kind of water remediation microbial inoculum and preparation method thereof
Technical field
The present invention relates to water protection field, in particular to a kind of water remediation microbial inoculum and preparation method thereof.
Background technology
With the development of society, problem of environmental pollution is increasingly serious, wherein, water pollution problem is increasingly severe
Have influence on the every aspect of human lives' production.At present, the lake in the whole nation 75% occurs in that different degrees of eutrophication, 90%
Urban waters it is seriously polluted, especially in southern city, the 60%-70% of total water deficit is caused due to water pollution,
Investigation according to official's data shows that 118 underground water of big and medium-sized cities of China have 115 urban groundwaters to be subject to different journeys
The pollution of degree, wherein, serious pollution accounts for 40%.
For above-mentioned water pollution problem, although have taking common physical method and chemical method to be administered at present
And reparation, but its high cost, produce effects undesirable, but also easily cause secondary pollution.
The content of the invention
It is an object of the invention to provide a kind of water remediation agent, it effectively can be repaired to polluted-water, and
Secondary pollution is not resulted in.
Another object of the present invention is to provide a kind of preparation method of water remediation microbial inoculum, its process is simple, low cost,
Prepared water remediation microbial inoculum effect is significant, is conducive to it to carry out popularization and application in water protection field.
The present invention solves its technical problem to be realized using following technical scheme.
The present invention proposes a kind of water remediation microbial inoculum, and it includes microbe carrier and microbial bacterial agent, wherein, microbial bacteria
Agent includes photosynthetic bacteria group, lactobacillus and yeast flora.
The present invention also proposes a kind of preparation method of water remediation microbial inoculum, and it is comprised the following steps:
Photosynthetic bacteria group, lactobacillus and yeast flora are each configured to bacteria suspension, are well mixed, obtain composite bacteria liquid;
Composite bacteria liquid is mixed with microbe carrier.
The beneficial effect of the water remediation microbial inoculum of the embodiment of the present invention and preparation method thereof is:By in water remediation microbial inoculum
Middle use photosynthetic bacteria group, lactobacillus and yeast flora, harmful substance that can be in Degradation and Transformation polluted-water simultaneously suppress harmful
The growth of microorganism and breeding, so as to reach the purpose repaired to polluted-water;In addition, by water remediation microbial inoculum
The microbe carrier added for the growth of photosynthetic bacteria group, lactobacillus and yeast flora can further improve each in water remediation microbial inoculum
The activity of microorganism is planted, so as to improve the quality of water remediation microbial inoculum on the whole.
Specific embodiment
To make the purpose, technical scheme and advantage of the embodiment of the present invention clearer, below will be in the embodiment of the present invention
Technical scheme be clearly and completely described.Unreceipted actual conditions person, builds according to normal condition or manufacturer in embodiment
The condition of view is carried out.Agents useful for same or the unreceipted production firm person of instrument, are the conventional product that can be obtained by commercially available purchase
Product.
Water remediation microbial inoculum of the embodiment of the present invention and preparation method thereof is specifically described below.
A kind of water remediation microbial inoculum provided in an embodiment of the present invention, it is mainly used in making when repairing contaminated water body
With.This water remediation microbial inoculum includes microbe carrier and microbial bacterial agent, wherein, microbial bacterial agent includes photosynthetic bacteria group, lactic acid
Flora and yeast flora.
Further, why photosynthetic bacteria group is added in above-mentioned microbial bacterial agent, its main cause there are two aspects:First
Aspect, be also it is mostly important a bit, i.e. photosynthetic bacteria group can degrade organic matter, nitrite, the sulfide being harmful in water body
The purpose purified water with the material such as ammonia, realization;Second aspect, adaptability is extremely strong in itself for photosynthetic bacteria group, can restrain oneself high concentration
Organic wastewater, have to noxious materials such as phenol, cyanogen it is very strong stand and decomposition and inversion ability, and because it belongs to independent nutrition
Microorganism, thalline contain more than 60% protein in itself, rich in multivitamin particularly microorganism B12, folic acid, biotin
Content is thousands of times of yeast, also contains Co-Q10, antiviral substance and promotion growth factor and physiological activator etc., institute
Itself acting as bait with photosynthetic bacteria group just has the effect for promoting biological growth and prevention from suffering from the diseases in water body, and this is for maintaining water body
The stabilization of microenvironment has important facilitation.
Further, lactobacillus are also included in microbial bacterial agent.It should be noted that the addition of lactobacillus is mainly
By the important biomolecule that it has is worth:Lactobacillus in the water body can by water body in the normal life of aquatic animal live
It is dynamic to enter into the enteron aisle of aquatic animal, and then lactobacillus can be adjusted to the flora Stable State Environment in aquatic animal enteron aisle
Section, improves the digestibility and biological value of aquatic animal successively;In addition, lactobacillus can also control the toxin in aquatic animal,
Suppress the generation of the growth and breeding and spoilage product of spoilage organisms in its enteron aisle.
Further, yeast flora is added in microbial bacterial agent to be mainly used for being supplied to other effective biological floras to increase
Required food is grown, so as to promote the normal growth of other biological bacterias to breed.In addition, it is necessary to explanation, yeast flora is also
Effective nutriment of the single celled albumen as other microorganisms or aquatic livestock needed for daily can be produced.
In addition, it is necessary to, it is emphasized that in order that the multiple-microorganism flora in mentioned microorganism microbial inoculum maintains one relatively
The state of stabilization, allows also for the special role of every kind of flora and the interaction relationship between them, and the present invention is implemented
Part by weight in the microbial bacterial agent that example is provided to several floras is limited, in particular:Photosynthetic bacteria group, lactobacillus
It is 180-260 with the mass ratio of yeast flora:65-86:22-33, preferably proportionate relationship are 220:75:28, therefore by many
The proportionate relationship for planting microorganism species is limited, it is possible to achieve the efficiency of microbial bacterial agent is maximized, so that preferably to water
Body is repaired.
Further, in order to improve various microorganism species activity, so as to the growth and breeding that they are preferably carried out, this hair
Various microorganism species are returned in the water remediation microbial inoculum that bright embodiment is provided there is provided microbe carrier.Need explanation
It is, the characteristics of in order to better adapt to microflora organisms small volume, it is preferable that the microbe carrier of the embodiment of the present invention is nanometer
Powder shaped carrier, specifically, can be selected from common graphene nano powder and nano TiO 2 powder.Need strong
Adjust, in order to keep the balanced relation of microbial bacterial agent and microbe carrier in water remediation microbial inoculum, the embodiment of the present invention pair
Both proportionate relationships are limited, specifically in parts by weight, 60-120 parts of microbial bacterial agent in water remediation microbial inoculum
30-60 parts of microbe carrier of collocation.
Further, in the middle of present pre-ferred embodiments, in order to improve the antibacterial ability of water remediation microbial inoculum, water body is repaiied
12-16 parts of organic acid is also added with multiple microbial inoculum.Preferably, the organic acid for being added is included in chlorogenic acid and corter pseudolaricis acid
It is at least one.It should be noted that chlorogenic acid and corter pseudolaricis acid are the organic acids with certain bioactivity, wherein, the native rose of Sharon
Skin acid can resist suppression harmful fungoid, and chlorogenic acid not only has antibacterial action, the also effect of cholagogic and rising white blood cell,
Therefore, a certain amount of organic acid is added in water remediation microbial inoculum, both can further improves water quality environment, it is also possible to improve water
The constitution of lively thing, maintains the ecological balance of water body environment.
Further, in the middle of preferred embodiments of the present invention, 6-8 parts of biology is also added in water remediation microbial inoculum
Peptide.It should be noted that the biological peptide for being added includes at least one in antibacterial peptide and complex peptides.Wherein, antibacterial peptide master
Will or in order to further improve the antibiotic and sterilizing ability of water remediation microbial inoculum, water quality environment is improved with this, and complex peptides master
Refer to the multiple proteins such as animals and plants, aquatic products, livestock products mixture through the obtained complex peptides of enzymolysis, it is added to water remediation bacterium
It is mainly used for improving the lipid-metabolism function of aquatic animal in agent, helps it to improve intestinal environment, recovers and improve its digestion
Absorbability of the road for nutriment.
It should be strongly noted that because water remediation microbial inoculum provided in an embodiment of the present invention is carried out as market product
Sale is used, therefore, in order to meet its quality stability to carry out marketing, water body provided in an embodiment of the present invention is repaiied
Stabilizer is also added in multiple microbial inoculum, and this stabilizer is selected from potassium sorbate or Sodium Benzoate.
The embodiment of the present invention additionally provides a kind of preparation method of water remediation microbial inoculum, and it is comprised the following steps:
After photosynthetic bacteria group, lactobacillus and yeast flora are each configured into bacteria suspension, pour into slowly together and carry out
It is slowly stirred, after it is well mixed, is mixed with microbe carrier.It should be noted that during being slowly stirred,
Can be slowly stirred using glass bar, also can use the mode of magnetic agitation, magnetic agitation rotating speed is controlled at 60-80 revs/min.
Further, in order to lift the shelf characteric of prepared water remediation agent, it is preferable that in above-mentioned preparation process
In, also added stabilizer.Specifically, stabilizer selection potassium sorbate or Sodium Benzoate, addition the time be composite bacteria liquid with it is micro-
After bio-carrier is mixed.It should be noted that the addition of stabilizer causes that the holding time of water remediation microbial inoculum is prolonged
It is long, be conducive to the sales promotion of water remediation microbial inoculum.
Feature of the invention and performance are described in further detail with reference to embodiments.
Embodiment 1
The water remediation microbial inoculum that the present embodiment is provided, it is mainly used in repairing polluted-water, and it includes 3kg graphite
Alkene nano-powder, 6kg microbial bacterial agents, wherein, microbial bacterial agent includes photosynthetic bacteria group, lactobacillus and yeast flora, and
The mass ratio of three is 180:65:22.
The present embodiment additionally provides a kind of preparation method of water remediation microbial inoculum, and it is comprised the following steps:
First, 4.045kg photosynthetic bacteria groups, 1.461kg lactobacillus and 0.494kg yeast floras are each configured into bacterium to hang
After liquid, then, three kinds of bacteria suspensions is poured on and carry out magnetic agitation half an hour together and with 60 revs/min of rotating speed, finally, then
Mixed with 3kg graphene nano powders.
Embodiment 2
The water remediation microbial inoculum that the present embodiment is provided, it is mainly used in repairing polluted-water, and it includes 4.5kg stones
Black alkene nano-powder, 12kg microbial bacterial agents, wherein, microbial bacterial agent includes photosynthetic bacteria group, lactobacillus and yeast flora,
And the mass ratio of three is 180:65:22.
Embodiment 3
The water remediation microbial inoculum that the present embodiment is provided, it is mainly used in repairing polluted-water, and it includes 6kg graphite
Alkene nano-powder, 9kg microbial bacterial agents, wherein, microbial bacterial agent includes photosynthetic bacteria group, lactobacillus and yeast flora, and
The mass ratio of three is 180:65:22.
Embodiment 4
The water remediation microbial inoculum that the present embodiment is provided, it is mainly used in repairing polluted-water, and it includes 4.5kg stones
Black alkene nano-powder, 12kg microbial bacterial agents, wherein, microbial bacterial agent includes photosynthetic bacteria group, lactobacillus and yeast flora,
And the mass ratio of three is 260:86:33.
Embodiment 5
The water remediation microbial inoculum that the present embodiment is provided, it is mainly used in repairing polluted-water, and it includes 4.5kg stones
Black alkene nano-powder, 12kg microbial bacterial agents and 1.2kg chlorogenic acids, wherein, microbial bacterial agent includes photosynthetic bacteria group, lactic acid bacteria
Group and yeast flora, and the mass ratio of three is 260:86:33.
Embodiment 6
The water remediation microbial inoculum that the present embodiment is provided, it is mainly used in repairing polluted-water, and it includes 4.5kg stones
Black alkene nano-powder, 12kg microbial bacterial agents and 1.6kg chlorogenic acids, wherein, microbial bacterial agent includes photosynthetic bacteria group, lactic acid bacteria
Group and yeast flora, and the mass ratio of three is 260:86:33.
Embodiment 7
The water remediation microbial inoculum that the present embodiment is provided, it is mainly used in repairing polluted-water, and it includes 4.5kg stones
Black alkene nano-powder, 12kg microbial bacterial agents and 1.4kg chlorogenic acids, wherein, microbial bacterial agent includes photosynthetic bacteria group, lactic acid bacteria
Group and yeast flora, and the mass ratio of three is 260:86:33.
Embodiment 8
The water remediation microbial inoculum that the present embodiment is provided, it is mainly used in repairing polluted-water, and it includes 4.5kg stones
Black alkene nano-powder, 12kg microbial bacterial agents and 1.2kg corter pseudolaricis acids, wherein, microbial bacterial agent includes photosynthetic bacteria group, lactic acid
Flora and yeast flora, and the mass ratio of three is 260:86:33.
Embodiment 9
The water remediation microbial inoculum that the present embodiment is provided, it is mainly used in repairing polluted-water, and it includes 4.5kg stones
Black alkene nano-powder, 12kg microbial bacterial agents and 1.6kg corter pseudolaricis acids, wherein, microbial bacterial agent includes photosynthetic bacteria group, lactic acid
Flora and yeast flora, and the mass ratio of three is 260:86:33.
Embodiment 10
The water remediation microbial inoculum that the present embodiment is provided, it is mainly used in repairing polluted-water, and it includes 4.5kg stones
Black alkene nano-powder, 12kg microbial bacterial agents and 1.4kg corter pseudolaricis acids, wherein, microbial bacterial agent includes photosynthetic bacteria group, lactic acid
Flora and yeast flora, and the mass ratio of three is 260:86:33.
Embodiment 11
The water remediation microbial inoculum that the present embodiment is provided, it is mainly used in repairing polluted-water, and it includes 4.5kg stones
Black alkene nano-powder, 12kg microbial bacterial agents, 1.4kg corter pseudolaricis acids and 0.6kg antibacterial peptides, wherein, microbial bacterial agent includes
Photosynthetic bacteria group, lactobacillus and yeast flora, and the mass ratio of three is 260:86:33.
Embodiment 12
The water remediation microbial inoculum that the present embodiment is provided, it is mainly used in repairing polluted-water, and it includes 4.5kg stones
Black alkene nano-powder, 12kg microbial bacterial agents, 1.4kg corter pseudolaricis acids and 0.8kg antibacterial peptides, wherein, microbial bacterial agent includes
Photosynthetic bacteria group, lactobacillus and yeast flora, and the mass ratio of three is 260:86:33.
Embodiment 13
The water remediation microbial inoculum that the present embodiment is provided, it is mainly used in repairing polluted-water, and it includes 4.5kg stones
Black alkene nano-powder, 12kg microbial bacterial agents, 1.4kg corter pseudolaricis acids and 0.7kg antibacterial peptides, wherein, microbial bacterial agent includes
Photosynthetic bacteria group, lactobacillus and yeast flora, and the mass ratio of three is 260:86:33.
Embodiment 14
The water remediation microbial inoculum that the present embodiment is provided, it is mainly used in repairing polluted-water, and it includes 4.5kg stones
Black alkene nano-powder, 12kg microbial bacterial agents, 1.4kg corter pseudolaricis acids and 0.6kg complex peptides, wherein, microbial bacterial agent includes
Photosynthetic bacteria group, lactobacillus and yeast flora, and the mass ratio of three is 260:86:33.
Embodiment 15
The water remediation microbial inoculum that the present embodiment is provided, it is mainly used in repairing polluted-water, and it includes 4.5kg stones
Black alkene nano-powder, 12kg microbial bacterial agents, 1.4kg corter pseudolaricis acids and 0.8kg complex peptides, wherein, microbial bacterial agent includes
Photosynthetic bacteria group, lactobacillus and yeast flora, and the mass ratio of three is 260:86:33.
Embodiment 16
The water remediation microbial inoculum that the present embodiment is provided, it is mainly used in repairing polluted-water, and it includes 4.5kg stones
Black alkene nano-powder, 12kg microbial bacterial agents, 1.4kg corter pseudolaricis acids, 0.7kg complex peptides and 0.2kg Sodium Benzoates, wherein, it is micro-
Bacteria agent includes photosynthetic bacteria group, lactobacillus and yeast flora, and the mass ratio of three is 260:86:33.
The present embodiment additionally provides a kind of preparation method of water remediation microbial inoculum, and it is comprised the following steps:
First, 8.232kg photosynthetic bacteria groups, 2.723kg lactobacillus and 1.045kg yeast floras are each configured into bacterium to hang
After liquid, then, three kinds of bacteria suspensions is poured on and carry out magnetic agitation 40 minutes together and with 70 revs/min of rotating speed, finally again with
4.5kg graphene nano powders are mixed.
Embodiment 17
The water remediation microbial inoculum that the present embodiment is provided, it is mainly used in repairing polluted-water, and it includes 4.5kg stones
Black alkene nano-powder, 12kg microbial bacterial agents, 1.4kg corter pseudolaricis acids, 0.7kg complex peptides and 0.2kg Sodium Benzoates, wherein, it is micro-
Bacteria agent includes photosynthetic bacteria group, lactobacillus and yeast flora, and the mass ratio of three is 260:86:33.
The present embodiment additionally provides a kind of preparation method of water remediation microbial inoculum, and it is comprised the following steps:
First, 8.232kg photosynthetic bacteria groups, 2.723kg lactobacillus and 1.045kg yeast floras are each configured into bacterium to hang
After liquid, then, three kinds of bacteria suspensions is poured on and carry out magnetic agitation 40 minutes together and with 80 revs/min of rotating speed, finally, then with
4.5kg graphene nano powders are mixed.
Embodiment 18
The water remediation microbial inoculum that the present embodiment is provided, it is mainly used in repairing polluted-water, and it includes 4.5kg bis-
Nano titanium oxide powder, 12kg microbial bacterial agents, 1.4kg corter pseudolaricis acids, 0.7kg complex peptides and 0.2kg Sodium Benzoates, wherein,
Microbial bacterial agent includes photosynthetic bacteria group, lactobacillus and yeast flora, and the mass ratio of three is 260:86:33.
The present embodiment additionally provides a kind of preparation method of water remediation microbial inoculum, and it is comprised the following steps:
First, 8.232kg photosynthetic bacteria groups, 2.723kg lactobacillus and 1.045kg yeast floras are each configured into bacterium to hang
After liquid, then, three kinds of bacteria suspensions is poured on and carry out magnetic agitation 40 minutes together and with 70 revs/min of rotating speed, finally, then with
4.5kg nano TiO 2 powders are mixed.
Test example
In order to prove water remediation microbial inoculum that above-described embodiment 1-18 provides for beneficial effect that polluted-water is repaired
Really, the water remediation microbial inoculum that this test example selection embodiment 1-18 is provided is tested as sample, and comparison of design example is carried out
Control experiment.It should be noted that in comparative example, it is main that chemistry is carried out to polluted-water as flocculant using polyacrylamide
Treatment.
Further, polluted-water chooses the untreated effluent of papermaking printing enterprise.After amount removes 19 parts of waste water of equivalent, phase
The different water remediation reagents of equivalent are added dropwise simultaneously under the conditions of, treatment effect test periodically are carried out to it, as a result such as the institute of table 1
Show.
The effect performance repaired to waste water using different water remediation reagents in the test example of table 1
As it can be seen from table 1 for the water remediation microbial inoculums that are provided of embodiment 1-18 are compared to comparative example, either
Water remediation speed or water remediation effect are intended to more excellent than the polyacrylamide performance that comparative example is used.Specifically
Ground, in water remediation speed, the muddiness that has of the water remediation microbial inoculum that embodiment 18 is provided performance intuitively arrives clarification
Speed wants fast comparative example speed close to 3 times, and continues from the point of view of repairing effect of degrading, and embodiment 18 is provided in its same time
Water remediation microbial inoculum best results, the polyacrylamide treatment effect used in comparative example is worst.Therefore, on the whole, originally
The water remediation microbial inoculum that inventive embodiments are provided is fast for sewage disposal degradation speed, and effect is extremely obvious.In addition, micro- life
Thing degrades, and treatment waste water is apparent compared with chemical treatment also to be had low cost and not to cause the advantage of secondary pollution.
In sum, the embodiment of the present invention is by using photosynthetic bacteria group, lactobacillus and yeast flora so that water remediation
Microbial inoculum can fast degradation conversion polluted-water in harmful substance and suppress harmful microbe growth with breeding, so as to reach
The purpose repaired to polluted-water;By adding a certain amount of organic acid and biological peptide in water remediation microbial inoculum, and match somebody with somebody
Conjunction uses stabilizer so that comprehensive effect of aquatic products probiotics is further lifted;In addition, adding by water remediation microbial inoculum
Plus improve various microorganisms in water remediation microbial inoculum for the microbe carrier of photosynthetic bacteria group, lactobacillus and yeast flora growth
Activity, so as to improve the quality of water remediation microbial inoculum on the whole.
Embodiments described above is a part of embodiment of the invention, rather than whole embodiments.Reality of the invention
The detailed description for applying example is not intended to limit the scope of claimed invention, but is merely representative of selected implementation of the invention
Example.Based on the embodiment in the present invention, what those of ordinary skill in the art were obtained under the premise of creative work is not made
Every other embodiment, belongs to the scope of protection of the invention.