CN110282755A - A kind of formula and preparation method thereof of water remediation microbial inoculum - Google Patents
A kind of formula and preparation method thereof of water remediation microbial inoculum Download PDFInfo
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- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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Abstract
The invention discloses a kind of formulas and preparation method thereof of water remediation microbial inoculum, more particularly to sewage treatment field, including microbial bacterial agent and carrier, wherein microbial bacterial agent includes bacterial digestion, denitrifying bacteria, fibrosis Nocard's bacillus, lactobacillus plantarum, produces Ruan's Candida, bacillus subtilis and colloid bacillus cereus, and wherein carrier includes wheat bran, zein and zeolite powder.By adding composite bacteria agent in water remediation microbial inoculum, composite bacteria agent easily lives and reproduces the present invention, forms beneficial advantage group, regulates and controls microbial ecological structure in water body；The harmful substance in contaminant water can not only be degraded and be converted to microbial inoculum, promote the growth of indigenous flora, play the role of purifying water, the multiple microbial strains group energy more importantly contained in microbial inoculum promotes the growth of water plant, prevent and treat aquatic animal disease, the excessive multiplication for inhibiting algae is finally reached the effect stablized and repair water ecological setting.
Description
Technical field
The present invention relates to technical field of sewage, it is more particularly related to which a kind of water remediation microbial inoculum is matched
Side and preparation method thereof.
Background technique
River, the water body in lake itself have adaptive and self-regulation ability, can purify the pollution being discharged into water
Object makes water body be restored to original ecological functions.However, with the rapid development of modern society, the improvement of people's living standards,
Industrial wastewater and sanitary sewage amount proportional increase are discharged into the amount of pollutant in water body far more than the carrying energy of water environment
Power, Ecology are gradually degenerated by serious destruction, the function of the ecosystem, and diversity of organism is also reduced therewith.Water environment
The unbalance stabilization for affecting environment for human survival, while restrict the lasting development of human society.Therefore how reasonable,
Water pollution is effectively administered, how to improve and rehabilitate water ecology, is the major issue for realizing Faced In Sustainable Development.
Currently, the method for manually repairing water body mainly has physical method, chemical method and bioanalysis.Common physical method and
When chemical method administers and repairs polluted-water, usually there is treatment cost height, produce effects undesirable, cause asking for secondary pollution etc.
Topic.It is the new technology risen in recent years that bioanalysis, which repairs water body, microorganism is mainly added in polluted-water, or pass through people
The method of indigenous microorganism in work culture water, absorbed using microorganism, converted or degraded water pollutant, reaches elimination pollution,
Restore the biological control measure of water ecology function.The advantage that bioanalysis repairs water ecology is that cost of investment is lower, and effect is good, no
Easily cause pollution administration.
But the method that existing bioanalysis repairs water ecology generally carries out reparation water body using water remediation microbial inoculum,
But production technology is complicated, repairing effect is little.
Summary of the invention
In order to overcome the drawbacks described above of the prior art, the embodiment of the present invention provide a kind of water remediation microbial inoculum formula and
Preparation method, by adding composite bacteria agent in water remediation microbial inoculum, composite bacteria agent is easily lived and reproduced, and forms beneficial advantage
Group regulates and controls microbial ecological structure in water body；The harmful substance in contaminant water can not only be degraded and be converted to microbial inoculum, promote original inhabitants
The growth of flora is played the role of purifying water, it is often more important that the multiple microbial strains group energy contained in microbial inoculum promotes aquatic
The growth of plant prevents and treats aquatic animal disease, inhibits the excessive multiplication of algae, is finally reached and stablizes and repair water ecological setting
Effect.
To achieve the above object, the invention provides the following technical scheme: a kind of formula of water remediation microbial inoculum, including micro- life
Object microbial inoculum and carrier, wherein microbial bacterial agent include bacterial digestion, denitrifying bacteria, fibrosis Nocard's bacillus, lactobacillus plantarum,
Ruan's Candida, bacillus subtilis and colloid bacillus cereus are produced, wherein carrier includes wheat bran, zein and zeolite powder.
In a preferred embodiment, the wheat bran, zein, zeolite powder and microbial bacterial agent be (in parts by weight
Meter) specifically: 10-30 parts of wheat bran, 6-10 parts of zein, 40-60 parts of zeolite powder, 8-10 parts of microbial bacterial agent.
In a preferred embodiment, every kind of bacteria suspension viable count is 1.0 × 108-1.0 × 109cfu/mL.
In a preferred embodiment, the nitrobacteria, denitrifying bacteria, lactobacillus plantarum, Candida utilis ferment
Mother, fibrosis Nocard's bacillus, bacillus subtilis and colloid bacillus cereus mass ratio are (2-4): (2-4): (4-8): (6-
9): (4-6): (1-4): (2-4).
In a preferred embodiment, the nitrobacteria, denitrifying bacteria, lactobacillus plantarum, Candida utilis ferment
Mother, fibrosis Nocard's bacillus, bacillus subtilis and colloid bacillus cereus mass ratio are (2-4): (2-4): (4-8): (6-
9): (4-6): (1-4): (2-4).
A kind of preparation method of water remediation microbial inoculum, specifically includes the following steps:
S1, by nitrobacteria, denitrifying bacteria, lactobacillus plantarum, candida utili, fibrosis Nocard's bacillus, withered grass
Bacteria suspension is respectively prepared in bacillus and colloid bacillus cereus；
S2, each bacteria suspension made of step S1 is uniformly mixed, obtains plastc ring；
S3, wheat bran, zein and zeolite powder are stirred, obtain powder carrier；
S4, the plastc ring that step S2 is obtained is uniformly mixed with the powder carrier that step S3 is obtained, water can be obtained
Body remediation microbial inoculum finished product.
In a preferred embodiment, it after the plastc ring is mixed with the powder carrier, is additionally added
Stabilizer, the stabilizer are one of potassium sorbate or sodium benzoate.
The invention also includes the application method of water remediation microbial inoculum, specifically used step are as follows: water remediation microbial inoculum finished product exists
4-6h is activated at room temperature using molasses water using preceding.
Technical effect and advantage of the invention:
1, the present invention is by adding composite bacteria agent in water remediation microbial inoculum, the pole in water environment of each flora in composite bacteria agent
It easily lives and reproduces, can quickly and steadily occupy the ecologic niche in water environment, form beneficial advantage group, regulate and control in water body
Microbial ecological structure；The harmful substance in contaminant water can not only be degraded and be converted to microbial inoculum, promote the growth of indigenous flora, play
The effect to purify water, it is often more important that the multiple microbial strains group energy contained in microbial inoculum promotes the growth of water plant, prevention and treatment
Aquatic animal disease inhibits the excessive multiplication of algae, is finally reached the effect stablized and repair water ecological setting；
2, the microbe species that water remediation microbial inoculum of the present invention is included are more, a certain amount of for micro- by adding in microbial inoculum
The mixed carrier of biological growth and suitable stabilizer can play and improve microbial inoculum repairing effect, be convenient for storage and transport, be conducive to
The effect of marketing；
3, Nitrite transformation can be nitrate by nitrobacteria, can not only reduce the content of Nitrite,
Mitigate and even release murder by poisoning of the nitrite to animals and plants in water completely, but also the content of nitrate in water body can be increased,
Promote the growth of advantageous algae in water body, reduce the alkaline matter in water body, increase acidic materials, reduces pH value；
4, denitrifying bacteria can not only restore the nitrite in water body using nitrite nitrogen and nitrate nitrogen as nitrogen source,
It is allowed to generate harmless nitrogen, releases the harm of Nitrite too high levels；Water element can also largely be consumed
Algae nitrogen nutrition is cut off in nutrition, is inhibited the inhibition of Measures of Algae in Water Body excessive multiplication, is played the role of purifying water body.Not only such as
This, denitrifying bacteria can survive under dissolved oxygen abundance and anaerobic condition and carry out anti-nitration reaction, optimize the object of water bottom
Reason, chemical environment；
5, the difference of lactobacillus plantarum and other lactobacillus is that its viable count is relatively high, can largely produce acid, make water
In pH stable do not increase, and the acidic materials of its output can degrade heavy metal；Lactobacillus plantarum is anaerobic bacteria, numerous
The energy distinctive a kind of preservative-lactobacillin of bion of output during growing can inhibit water bottom aquatic dynamic well
Object excrement and residual bait material rot, to reduce the increase of ammonia nitrogen and nitrite in water；
6, candida utili can be using urea and nitric acid as nitrogen source, and not needing any growth factor is added can grow,
Can be used for being supplied to nutrient required for other effective biological floras are grown, in addition, candida utili can also generate it is slender
The albumen of born of the same parents provides daily required nutriment for other aquatic animals or microorganism；
7, nitre fibrosis Nocard's bacillus can reduce the content of hydro carbons and inorganic cyanide in water body, adhere to bacterium glue thereon
Group forms mutualism with trichobacteria, can maintain the stability of water remediation process, in addition to this, fibrosis Nocard's bacillus
It can promote the growth of plant itself, and enhance the fertility of bed mud, secondary metabolite can be synthesized in growth course, inhibit water
The growth of middle harmful bacteria；
8, bacillus subtilis can secrete a large amount of chitinases, decompose the cell wall of disease fungus and inhibit nosomycosis
Evil；Inhibit the harmful microorganisms such as vibrios, Escherichia coli and baculoviral in water body；Decompose the organic matter and nuisance in aquaculture pond
Matter decomposes rubbish little particle in water, improves water turbidity problem caused by harmful cyanobacteria is overflowed, to play the work to purify water
With；Can generate has stronger protease, lipase, amylase, promotes nutrient degradation in feed, makes aquatic animal to feed
Be absorbed and utilized more sufficiently；
9, colloid bacillus cereus can decompose potassium, silicon and phosphorus in mineral, have the function of Soluble phosphorus, Potassium release and fixed nitrogen, in thallus
Potassium after thallus is dead, separate out can be absorbed and utilized by water plant；It can be generated during its growth and breeding organic
Acid, amino acid, polysaccharide, hormone etc. are conducive to the substance that water plant absorbs and utilizes；Plant growth substance and more can be secreted
Kind enzyme inhibits the growth of other pathogens while enhancing plant to some disease resistances；
10, in order to promote various flora growths and breeding, make flora that there is more high activity, the present invention is water remediation microbial inoculum
In the carrier that provides of various microbial floras, in order to better adapt to microflora organisms feature small in size, microorganism of the present invention
Carrier be zeolite powder, wheat bran and zein, to the proportionate relationship of microbial bacterial agent and microbe carrier in water remediation microbial inoculum into
It has gone limitation, has maintained the balanced relation of microbial bacterial agent and microbe carrier in water remediation microbial inoculum；
11, by also added stabilizer in water remediation microbial inoculum, and this stabilizer is potassium sorbate or sodium benzoate
One of, convenient for the marketing of water remediation microbial inoculum, need that certain methods is used to stablize with the quality for guaranteeing microbial inoculum product
Property, to meet the needs of production, preservation and market sale.
Specific embodiment
Below in conjunction with the embodiment in the present invention, technical solution in the embodiment of the present invention is carried out clearly and completely
Description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on this hair
Embodiment in bright, every other implementation obtained by those of ordinary skill in the art without making creative efforts
Example, shall fall within the protection scope of the present invention.
Embodiment 1:
The present invention provides a kind of formula of water remediation microbial inoculum, including microbial bacterial agent and carrier, wherein microbial bacteria
Agent includes bacterial digestion, denitrifying bacteria, fibrosis Nocard's bacillus, lactobacillus plantarum, produces Ruan's Candida, bacillus subtilis
And colloid bacillus cereus, wherein carrier includes wheat bran, zein and zeolite powder；
The wheat bran, zein, zeolite powder and microbial bacterial agent (according to parts by weight) specifically: 10-30 parts of wheat bran, jade
Rice 6-10 parts of protein, 40-60 parts of zeolite powder, 8-10 parts of microbial bacterial agent；
Every kind of bacteria suspension viable count is 1.0 × 108-1.0 × 109cfu/mL；
The nitrobacteria, denitrifying bacteria, lactobacillus plantarum, candida utili, fibrosis Nocard's bacillus, withered grass bud
Spore bacillus and colloid bacillus cereus mass ratio are (2-4): (2-4): (4-8): (6-9): (4-6): (1-4): (2-4)；
And specific in the present embodiment, the formula (by weight) of water remediation microbial inoculum includes: 5kg wheat bran, 3kg corn
Protein, 20kg zeolite powder and 4kg composite bacteria agent, wherein nitrobacteria, denitrifying bacteria, lactobacillus plantarum, Candida utilis ferment
Mother, fibrosis Nocard's bacillus, bacillus subtilis and colloid bacillus cereus mass ratio be 2:2:4:6:4:1:2；
Every kind of bacteria suspension viable count is 1.0 × 108cfu/mL；
The preparation method of water remediation microbial inoculum is specific as follows:
S1, by nitrobacteria, denitrifying bacteria, lactobacillus plantarum, candida utili, fibrosis Nocard's bacillus, withered grass
Bacteria suspension is respectively prepared in bacillus and colloid bacillus cereus；
S2, each bacteria suspension made of step S1 is uniformly mixed, obtains plastc ring；
S3, wheat bran, zein and zeolite powder are stirred, obtain powder carrier；
S4, the plastc ring that step S2 is obtained is uniformly mixed with the powder carrier that step S3 is obtained, water can be obtained
Body remediation microbial inoculum finished product；
The specifically used step of water remediation microbial inoculum are as follows: water remediation microbial inoculum finished product is utilizing molasses water in room temperature item using preceding
4h is activated under part.
Embodiment 2:
The present invention provides a kind of formula of water remediation microbial inoculum, including microbial bacterial agent and carrier, wherein microbial bacteria
Agent includes bacterial digestion, denitrifying bacteria, fibrosis Nocard's bacillus, lactobacillus plantarum, produces Ruan's Candida, bacillus subtilis
And colloid bacillus cereus, wherein carrier includes wheat bran, zein and zeolite powder；
The wheat bran, zein, zeolite powder and microbial bacterial agent (according to parts by weight) specifically: 10-30 parts of wheat bran, jade
Rice 6-10 parts of protein, 40-60 parts of zeolite powder, 8-10 parts of microbial bacterial agent；
Every kind of bacteria suspension viable count is 1.0 × 108-1.0 × 109cfu/mL；
The nitrobacteria, denitrifying bacteria, lactobacillus plantarum, candida utili, fibrosis Nocard's bacillus, withered grass bud
Spore bacillus and colloid bacillus cereus mass ratio are (2-4): (2-4): (4-8): (6-9): (4-6): (1-4): (2-4)；
And specific in the present embodiment, the formula (by weight) of water remediation microbial inoculum includes: 3kg wheat bran, 1kg corn
Protein, 6kg zeolite powder and 1kg composite bacteria agent, wherein nitrobacteria, denitrifying bacteria, lactobacillus plantarum, candida utili,
The mass ratio of fibrosis Nocard's bacillus, bacillus subtilis and colloid bacillus cereus is 2:2:4:6:4:1:2；
Every kind of bacteria suspension viable count is 1.0 × 109cfu/mL；
The preparation method of water remediation microbial inoculum is specific as follows:
S1, by nitrobacteria, denitrifying bacteria, lactobacillus plantarum, candida utili, fibrosis Nocard's bacillus, withered grass
Bacteria suspension is respectively prepared in bacillus and colloid bacillus cereus；
S2, each bacteria suspension made of step S1 is uniformly mixed, obtains plastc ring；
S3, wheat bran, zein and zeolite powder are stirred, obtain powder carrier；
S4, the plastc ring that step S2 is obtained is uniformly mixed with the powder carrier that step S3 is obtained, water can be obtained
Body remediation microbial inoculum finished product；
The specifically used step of water remediation microbial inoculum are as follows: water remediation microbial inoculum finished product is utilizing molasses water in room temperature item using preceding
5h is activated under part.
Embodiment 3:
The present invention provides a kind of formula of water remediation microbial inoculum, including microbial bacterial agent and carrier, wherein microbial bacteria
Agent includes bacterial digestion, denitrifying bacteria, fibrosis Nocard's bacillus, lactobacillus plantarum, produces Ruan's Candida, bacillus subtilis
And colloid bacillus cereus, wherein carrier includes wheat bran, zein and zeolite powder；
The wheat bran, zein, zeolite powder and microbial bacterial agent (according to parts by weight) specifically: 10-30 parts of wheat bran, jade
Rice 6-10 parts of protein, 40-60 parts of zeolite powder, 8-10 parts of microbial bacterial agent；
Every kind of bacteria suspension viable count is 1.0 × 108-1.0 × 109cfu/mL；
The nitrobacteria, denitrifying bacteria, lactobacillus plantarum, candida utili, fibrosis Nocard's bacillus, withered grass bud
Spore bacillus and colloid bacillus cereus mass ratio are (2-4): (2-4): (4-8): (6-9): (4-6): (1-4): (2-4)；
And specific in the present embodiment, the formula (by weight) of water remediation microbial inoculum includes: 5kg wheat bran, 3kg corn
Protein, 20kg zeolite powder and 4kg composite bacteria agent, wherein nitrobacteria, denitrifying bacteria, lactobacillus plantarum, Candida utilis ferment
Mother, fibrosis Nocard's bacillus, bacillus subtilis and colloid bacillus cereus mass ratio be 4:4:8:9:6:4:4；
Every kind of bacteria suspension viable count is 1.0 × 109cfu/mL；
The preparation method of water remediation microbial inoculum is specific as follows:
S1, by nitrobacteria, denitrifying bacteria, lactobacillus plantarum, candida utili, fibrosis Nocard's bacillus, withered grass
Bacteria suspension is respectively prepared in bacillus and colloid bacillus cereus；
S2, each bacteria suspension made of step S1 is uniformly mixed, obtains plastc ring；
S3, wheat bran, zein and zeolite powder are stirred, obtain powder carrier；
S4, the plastc ring that step S2 is obtained is uniformly mixed with the powder carrier that step S3 is obtained, water can be obtained
Body remediation microbial inoculum finished product；
The specifically used step of water remediation microbial inoculum are as follows: water remediation microbial inoculum finished product is utilizing molasses water in room temperature item using preceding
6h is activated under part.
Embodiment 4:
The present invention provides a kind of formula of water remediation microbial inoculum, including microbial bacterial agent and carrier, wherein microbial bacteria
Agent includes bacterial digestion, denitrifying bacteria, fibrosis Nocard's bacillus, lactobacillus plantarum, produces Ruan's Candida, bacillus subtilis
And colloid bacillus cereus, wherein carrier includes wheat bran, zein and zeolite powder；
The wheat bran, zein, zeolite powder and microbial bacterial agent (according to parts by weight) specifically: 10-30 parts of wheat bran, jade
Rice 6-10 parts of protein, 40-60 parts of zeolite powder, 8-10 parts of microbial bacterial agent；
Every kind of bacteria suspension viable count is 1.0 × 108-1.0 × 109cfu/mL；
The nitrobacteria, denitrifying bacteria, lactobacillus plantarum, candida utili, fibrosis Nocard's bacillus, withered grass bud
Spore bacillus and colloid bacillus cereus mass ratio are (2-4): (2-4): (4-8): (6-9): (4-6): (1-4): (2-4)；
And specific in the present embodiment, the formula (by weight) of water remediation microbial inoculum includes: 3kg wheat bran, 1kg corn
Protein, 6kg zeolite powder and 1kg composite bacteria agent, wherein nitrobacteria, denitrifying bacteria, lactobacillus plantarum, candida utili,
The mass ratio of fibrosis Nocard's bacillus, bacillus subtilis and colloid bacillus cereus is 4:4:8:9:6:4:4；
Every kind of bacteria suspension viable count is 1.0 × 109cfu/mL；
The preparation method of water remediation microbial inoculum is specific as follows:
S1, by nitrobacteria, denitrifying bacteria, lactobacillus plantarum, candida utili, fibrosis Nocard's bacillus, withered grass
Bacteria suspension is respectively prepared in bacillus and colloid bacillus cereus；
S2, each bacteria suspension made of step S1 is uniformly mixed, obtains plastc ring；
S3, wheat bran, zein and zeolite powder are stirred, obtain powder carrier；
S4, the plastc ring that step S2 is obtained is uniformly mixed with the powder carrier that step S3 is obtained, water can be obtained
Body remediation microbial inoculum finished product；
The specifically used step of water remediation microbial inoculum are as follows: water remediation microbial inoculum finished product is utilizing molasses water in room temperature item using preceding
6h is activated under part.
Embodiment 5:
The present invention provides a kind of formula of water remediation microbial inoculum, including microbial bacterial agent and carrier, wherein microbial bacteria
Agent includes bacterial digestion, denitrifying bacteria, fibrosis Nocard's bacillus, lactobacillus plantarum, produces Ruan's Candida, bacillus subtilis
And colloid bacillus cereus, wherein carrier includes wheat bran, zein and zeolite powder；
The wheat bran, zein, zeolite powder and microbial bacterial agent (according to parts by weight) specifically: 10-30 parts of wheat bran, jade
Rice 6-10 parts of protein, 40-60 parts of zeolite powder, 8-10 parts of microbial bacterial agent；
Every kind of bacteria suspension viable count is 1.0 × 108-1.0 × 109cfu/mL；
The nitrobacteria, denitrifying bacteria, lactobacillus plantarum, candida utili, fibrosis Nocard's bacillus, withered grass bud
Spore bacillus and colloid bacillus cereus mass ratio are (2-4): (2-4): (4-8): (6-9): (4-6): (1-4): (2-4)；
And specific in the present embodiment, the formula (by weight) of water remediation microbial inoculum includes: 3kg wheat bran, 1kg corn
Protein, 6kg zeolite powder, 1kg composite bacteria agent and 0.2kg sodium benzoate, wherein nitrobacteria, denitrifying bacteria, plant cream bar
Bacterium, candida utili, fibrosis Nocard's bacillus, bacillus subtilis and colloid bacillus cereus mass ratio be 2:2:4:6:4:
1:2；
Every kind of bacteria suspension viable count is 1.0 × 109cfu/mL；
The preparation method of water remediation microbial inoculum is specific as follows:
S1, by nitrobacteria, denitrifying bacteria, lactobacillus plantarum, candida utili, fibrosis Nocard's bacillus, withered grass
Bacteria suspension is respectively prepared in bacillus and colloid bacillus cereus；
S2, each bacteria suspension made of step S1 is uniformly mixed, obtains plastc ring；
S3, wheat bran, zein and zeolite powder are stirred, obtain powder carrier；
S4, the plastc ring that step S2 is obtained is uniformly mixed with the powder carrier that step S3 is obtained, water can be obtained
Body remediation microbial inoculum finished product；
The specifically used step of water remediation microbial inoculum are as follows: water remediation microbial inoculum finished product is utilizing molasses water in room temperature item using preceding
6h is activated under part.
Embodiment 6:
The present invention provides a kind of formula of water remediation microbial inoculum, including microbial bacterial agent and carrier, wherein microbial bacteria
Agent includes bacterial digestion, denitrifying bacteria, fibrosis Nocard's bacillus, lactobacillus plantarum, produces Ruan's Candida, bacillus subtilis
And colloid bacillus cereus, wherein carrier includes wheat bran, zein and zeolite powder；
The wheat bran, zein, zeolite powder and microbial bacterial agent (according to parts by weight) specifically: 10-30 parts of wheat bran, jade
Rice 6-10 parts of protein, 40-60 parts of zeolite powder, 8-10 parts of microbial bacterial agent；
Every kind of bacteria suspension viable count is 1.0 × 108-1.0 × 109cfu/mL；
The nitrobacteria, denitrifying bacteria, lactobacillus plantarum, candida utili, fibrosis Nocard's bacillus, withered grass bud
Spore bacillus and colloid bacillus cereus mass ratio are (2-4): (2-4): (4-8): (6-9): (4-6): (1-4): (2-4)；
And specific in the present embodiment, the formula (by weight) of water remediation microbial inoculum includes: 3kg wheat bran, 1kg corn
Protein, 6kg zeolite powder, 1kg composite bacteria agent and 0.2kg potassium sorbate, wherein nitrobacteria, denitrifying bacteria, plant cream bar
Bacterium, candida utili, fibrosis Nocard's bacillus, bacillus subtilis and colloid bacillus cereus mass ratio be 4:4:8:9:6:
4:4；
Every kind of bacteria suspension viable count is 1.0 × 108.5cfu/mL；
The preparation method of water remediation microbial inoculum is specific as follows:
S1, by nitrobacteria, denitrifying bacteria, lactobacillus plantarum, candida utili, fibrosis Nocard's bacillus, withered grass
Bacteria suspension is respectively prepared in bacillus and colloid bacillus cereus；
S2, each bacteria suspension made of step S1 is uniformly mixed, obtains plastc ring；
S3, wheat bran, zein and zeolite powder are stirred, obtain powder carrier；
S4, the plastc ring that step S2 is obtained is uniformly mixed with the powder carrier that step S3 is obtained, water can be obtained
Body remediation microbial inoculum finished product；
The specifically used step of water remediation microbial inoculum are as follows: water remediation microbial inoculum finished product is utilizing molasses water in room temperature item using preceding
6h is activated under part.
It is emphasized that making above-mentioned while interaction between the special role and flora in view of each flora
Each flora in microbial inoculum maintains relatively steady state, in the embodiment of the present invention, limits the ratio of several floras in composite bacteria agent
Example, in particular: nitrobacteria, denitrifying bacteria, lactobacillus plantarum, candida utili, fibrosis Nocard's bacillus, withered grass bud
Spore bacillus and colloid bacillus cereus are (2-4) in mass ratio: (2-4): (4-8): and (6-9): (4-6): (1-4): (2-4) composition,
Preferably proportionate relationship is 3:3:6:8:5:3:3, therefore by the proportionate relationship of limitation multiple-microorganism flora, microbial inoculum may be implemented
To the optimization of water remediation effect.
Test example:
In order to prove that the water remediation microbial inoculum of the above embodiments 1-6 offer repairs the effect of polluted-water, this test example
It chooses the water remediation microbial inoculum that embodiment 1-6 is provided to be tested as sample, and comparison of design example carries out check experiment.It needs
Illustrate, in comparative example, do not add any microbial inoculum and medicament, other experimental conditions are identical as embodiment, obtained test number
According to as follows:
Ammonia-nitrogen content situation of change in 1 water of table
Total nitrogen content situation of change in 2 water of table
Total phosphorus content situation of change in 3 water of table
Permanganate index changes of contents in 4 water of table
| Test group | Before test | After test | Removal rate |
| Comparative example | 17.51 | 16.82 | 3.9 |
| Embodiment 1 | 17.51 | 12.53 | 28.4 |
| Embodiment 2 | 17.51 | 13.42 | 23.4 |
| Embodiment 3 | 17.51 | 12.29 | 29.8 |
| Embodiment 4 | 17.51 | 11.83 | 32.4 |
| Embodiment 5 | 17.51 | 10.85 | 38.0 |
| Embodiment 6 | 17.51 | 11.23 | 35.9 |
As seen from the above table, the harmful substance in contaminant water can be degraded and be converted to composite bacteria agent of the present invention, promote indigenous flora
Growth, play the role of purifying water.
Last: although the present invention is disclosed as above with preferred embodiment, however, it is not to limit the invention, any ability
Field technique personnel, without departing from the spirit and scope of the present invention, when can make a little modification and perfect therefore of the invention guarantor
Shield range, which is worked as, to be subjected to the definition of the claims.
Claims (7)
1. a kind of formula of water remediation microbial inoculum, it is characterised in that: including microbial bacterial agent and carrier, wherein microbial bacterial agent packet
It includes bacterial digestion, denitrifying bacteria, fibrosis Nocard's bacillus, lactobacillus plantarum, produce Ruan's Candida, bacillus subtilis and glue
Matter bacillus, wherein carrier includes wheat bran, zein and zeolite powder.
2. a kind of formula of water remediation microbial inoculum according to claim 1, it is characterised in that: the wheat bran, zein, boiling
Mountain flour and microbial bacterial agent (according to parts by weight) specifically: 10-30 parts of wheat bran, 6-10 parts of zein, 40-60 parts of zeolite powder,
8-10 parts of microbial bacterial agent.
3. a kind of formula of water remediation microbial inoculum according to claim 1, it is characterised in that: every kind of bacteria suspension viable bacteria
Number is 1.0 × 108-1.0 × 109cfu/mL.
4. a kind of formula of water remediation microbial inoculum according to claim 1, it is characterised in that: the nitrobacteria, anti-nitre
Change bacterium, lactobacillus plantarum, candida utili, fibrosis Nocard's bacillus, bacillus subtilis and colloid bacillus cereus quality
Ratio is (2-4): (2-4): (4-8): (6-9): (4-6): (1-4): (2-4).
5. a kind of preparation method of water remediation microbial inoculum, it is characterised in that: specifically includes the following steps:
S1, by nitrobacteria, denitrifying bacteria, lactobacillus plantarum, candida utili, fibrosis Nocard's bacillus, withered grass gemma
Bacteria suspension is respectively prepared in bacillus and colloid bacillus cereus；
S2, each bacteria suspension made of step S1 is uniformly mixed, obtains plastc ring；
S3, wheat bran, zein and zeolite powder are stirred, obtain powder carrier；
S4, the plastc ring that step S2 is obtained is uniformly mixed with the powder carrier that step S3 is obtained, water body can be obtained and repair
Multiple microbial inoculum finished product.
6. a kind of preparation method of water remediation microbial inoculum according to claim 5, it is characterised in that: the plastc ring
After being mixed with the powder carrier, it is also added into stabilizer, the stabilizer is one in potassium sorbate or sodium benzoate
Kind.
7. according to a kind of preparation method of water remediation microbial inoculum as claimed in any one of claims 5 to 6, it is characterised in that: also wrap
Include the application method of water remediation microbial inoculum, specifically used step are as follows: water remediation microbial inoculum finished product is existed using preceding using molasses water
4-6h is activated under room temperature.
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| CN110387368A (en) * | 2019-07-29 | 2019-10-29 | 上海山恒生态科技股份有限公司 | A kind of formula and preparation method thereof of landscape water body remediation microbial inoculum |
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| CN110835172A (en) * | 2019-12-05 | 2020-02-25 | 中国科学院兰州化学物理研究所盱眙凹土应用技术研发中心 | Method for preparing eutrophic water body remediation material by using decolored waste soil |
| CN111019864A (en) * | 2019-12-29 | 2020-04-17 | 海南卓越生物有限公司 | Preparation method of composite EM (effective microorganisms) flora for decontamination of aquaculture |
| CN111099750A (en) * | 2020-01-10 | 2020-05-05 | 济南大学 | Mixed bacterium adsorption material and preparation method and application thereof |
| CN111187124B (en) * | 2020-02-07 | 2022-03-11 | 中国科学院兰州化学物理研究所 | Method for preparing eutrophic water body remediation material by using oil shale semi-coke and application |
| CN111187124A (en) * | 2020-02-07 | 2020-05-22 | 中国科学院兰州化学物理研究所 | Method for preparing eutrophic water body remediation material by using oil shale semi-coke and application |
| CN111747531A (en) * | 2020-06-19 | 2020-10-09 | 北京爱尔斯生态环境工程有限公司 | Method for restoring water ecological environment based on interaction of microorganisms and aquatic plants |
| CN112079446A (en) * | 2020-08-17 | 2020-12-15 | 南京乐萱生态环境科技有限公司 | Microbial regulator for improving watercourse water transparency, preparation method and application |
| CN111925969A (en) * | 2020-09-07 | 2020-11-13 | 福泉环保城发展有限公司 | Water treatment microbial inoculum |
| CN114438069A (en) * | 2020-10-31 | 2022-05-06 | 中国石油化工股份有限公司 | Double microcarrier for culturing nitrobacteria and preparation method and application thereof |
| CN114438069B (en) * | 2020-10-31 | 2023-07-28 | 中国石油化工股份有限公司 | Double microcarriers for culturing nitrifying bacteria and preparation method and application thereof |
| CN112850884A (en) * | 2021-01-07 | 2021-05-28 | 大唐环境产业集团股份有限公司 | Short fiber immobilization carrier of activated sludge, and preparation method and application thereof |
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