CN111019864A - Preparation method of composite EM (effective microorganisms) flora for decontamination of aquaculture - Google Patents
Preparation method of composite EM (effective microorganisms) flora for decontamination of aquaculture Download PDFInfo
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- CN111019864A CN111019864A CN201911386517.8A CN201911386517A CN111019864A CN 111019864 A CN111019864 A CN 111019864A CN 201911386517 A CN201911386517 A CN 201911386517A CN 111019864 A CN111019864 A CN 111019864A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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Abstract
The invention discloses a preparation method of a composite EM flora for decontamination of aquaculture, which comprises the following steps: the composite EM flora comprises lactobacillus acidophilus, bacillus subtilis and candida oviposition. The invention ensures the activity of each strain and simultaneously plays a synergistic role by screening, culturing and optimizing local dominant strains, including strains with pollution reduction effect on culture water, such as spores, yeast, lactobacillus, photosynthetic bacteria and the like, and compounding carriers. Can effectively degrade various water toxins, decompose macromolecular organic matters and degrade toxic and harmful pollution sources, thereby inhibiting the breeding of harmful bacteria and reducing various water harmful indexes.
Description
Technical Field
The invention relates to the field of preparation methods of composite EM (effective microorganisms) flora, in particular to a preparation method of composite EM flora for decontamination of aquaculture.
Background
Residual baits left by a large amount of baits thrown in a water body, accumulation of a large amount of excreta of aquatic animals in the water body, continuous increase of culture density for pursuing income, large water body load caused by a disinfectant and drug residues used regularly and the like in aquaculture of aquatic products (freshwater shrimps, river shrimps, prawns, metapenaeus ensis, lutea, lobsters, crabs, sea cucumbers, frogs, loaches, eels, turtles, finless eels and sea fresh water fishes), and physical and chemical indexes of the culture water body are damaged, namely water quality deterioration becomes turbid, pond water turns green, black, reddens and even becomes bad and smelly. Leading to the massive production of harmful substances such as ammonia nitrogen and nitrite in water, particularly leading to the overproof of nitrite ammonia nitrogen, and seriously leading to the death of aquatic organisms caused by poisoning, therefore, microorganisms need to be put into the water body to realize the purification of the water body.
Disclosure of Invention
Aiming at the problems, the invention provides a preparation method of a composite EM flora for decontamination of aquaculture, which can effectively solve the problems in the background technology.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows: a composite EM flora for removing dirt in aquaculture comprises Lactobacillus acidophilus, Bacillus subtilis and Candida oviposition.
Further, the ratio of the strains to the raw materials is as follows: 90% of jade bran and 10% of composite EM bacterial liquid.
Further, lactobacillus acidophilus: b, bacillus subtilis: candida oviposition =1:1: 2.
A composite EM flora culture method for aquaculture decontamination comprises the following steps:
(1) respectively taking lactobacillus strains, bacillus subtilis strains and saccharomycete strains, and adding a liquid culture medium into the strain mixture to obtain a compound strain liquid;
(2) adding brown sugar into the lactobacillus bacterial liquid obtained in the previous step for propagation, and controlling temperature and humidity;
(3) adding sodium silicate, water, brown sugar, wheat bran and jade bran into a mixer, mixing uniformly, fermenting with the composite bacteria liquid, fermenting and propagating, wherein the fermentation process needs to be carried out by stacking and turning for 4 times, and one day is used each time;
(4) drying at low temperature by using a drying bed, sealing and storing for later use after fermentation is finished;
(5) adding the compound bacteria primary expanded strain, sodium silicate, water, brown sugar, wheat bran and jade bran into a mixer, uniformly mixing, controlling temperature and humidity, fermenting and propagating, wherein the compound bacteria primary expanded strain needs to be piled and turned over for 4 times in the fermentation process, and one day is used each time;
(6) putting the secondary expanded strain of the compound bacteria into a fermentation tank, mixing with molasses urea water, stirring and fermenting twice a day, wherein stirring is required in the fermentation process;
(7) and (4) pouring and sealing the fermented strains.
The invention has the beneficial effects that: the invention ensures the activity of each strain and simultaneously plays a synergistic role by screening, culturing and optimizing local dominant strains, including strains with pollution reduction effect on culture water, such as spores, yeast, lactobacillus, photosynthetic bacteria and the like, and compounding carriers. Can effectively degrade various water toxins, decompose macromolecular organic matters and degrade toxic and harmful pollution sources, thereby inhibiting the breeding of harmful bacteria and reducing various water harmful indexes.
Drawings
FIG. 1 is a flow chart of the primary expanded strain of the composite bacteria of the present invention;
FIG. 2 is a flow chart of the secondary expanded strain of the composite bacteria of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example (b):
a composite EM flora for removing dirt in aquaculture comprises Lactobacillus acidophilus, Bacillus subtilis and Candida oviposition.
Further, the ratio of the strains to the raw materials is as follows: 90% of jade bran and 10% of composite EM bacterial liquid.
Further, lactobacillus acidophilus: b, bacillus subtilis: candida oviposition =1:1: 2.
A composite EM flora culture method for aquaculture decontamination comprises the following steps:
(1) respectively taking lactobacillus strains, bacillus subtilis strains and saccharomycete strains, and adding a liquid culture medium into the strain mixture to obtain a compound strain liquid;
(2) adding brown sugar into the lactobacillus bacterial liquid obtained in the previous step for propagation, and controlling temperature and humidity;
(3) adding sodium silicate, water, brown sugar, wheat bran and jade bran into a mixer, mixing uniformly, fermenting with the composite bacteria liquid, fermenting and propagating, wherein the fermentation process needs to be carried out by stacking and turning for 4 times, and one day is used each time;
(4) drying at low temperature by using a drying bed, sealing and storing for later use after fermentation is finished;
(5) adding the compound bacteria primary expanded strain, sodium silicate, water, brown sugar, wheat bran and jade bran into a mixer, uniformly mixing, controlling temperature and humidity, fermenting and propagating, wherein the compound bacteria primary expanded strain needs to be piled and turned over for 4 times in the fermentation process, and one day is used each time;
(6) putting the secondary expanded strain of the compound bacteria into a fermentation tank, mixing with molasses urea water, stirring and fermenting twice a day, wherein stirring is required in the fermentation process;
(7) and (4) pouring and sealing the fermented strains.
The finished product has the characteristics that: light red or brownish red liquid, and fermented fragrance.
In the technical scheme, the beneficial thallus components can also comprise more than thirty composite microbial populations such as spore flora, lactic acid flora, yeast flora, actinomycete flora and the like and health-care products for metabolism, antibiosis and digestion promotion: digestive enzymes, lactic acid, and antibiotics (no antagonism, no residue) are unknown growth-promoting factors.
The implementation and use method comprises the following steps:
the content of each bottle is 1L, the total viable count of the thallus component is more than or equal to 2000 hundred million/cfu,
conventional water culture: the product is added into 1 bottle of the product and is soaked in water for 2-3 hours, and then the product is sprinkled in the morning.
Shrimp, crab and eel ponds: 4-6 mu of rice is used per bottle, and 7-10 mu of rice is used per bottle in the fish and sea cucumber pond. Biological pond cleaning: each bottle can be used for 10-12 mu; treating water qualities such as red turbidity, white turbidity, black turbidity, thick turbidity, algae phase aging and the like: if the water quality is seriously deteriorated by sprinkling the product, the product can be used for 2 times within 3 days. Elimination of gill changes, healthy gill: mixing the product in 1 bottle with appropriate amount of zeolite powder, and spraying into water for 2 days 1 time per day.
Based on the above, the invention has the advantages that the invention adopts advanced microbial engineering technology, scientifically and reasonably configures the carrier culture medium, and ensures the activity of each strain and simultaneously exerts the synergistic effect thereof by screening, culturing and optimizing the local dominant strains, including strains with pollution reduction effect on the culture water body, such as spores, yeast, lactobacillus, photosynthetic bacteria and the like, and compounding the carrier. Can effectively degrade various water toxins, decompose macromolecular organic matters and degrade toxic and harmful pollution sources, thereby inhibiting the breeding of harmful bacteria and reducing various water harmful indexes.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (6)
1. A complex EM flora for decontamination of aquaculture, characterized by: the composite EM flora comprises lactobacillus acidophilus, bacillus subtilis and candida oviposition.
2. The composite EM flora for decontamination of aquaculture as claimed in claim 1, wherein the ratio of species to feed is: 90% of jade bran and 10% of composite EM bacterial liquid.
3. The complex EM flora for aquaculture decontamination as claimed in claim 1, wherein the lactobacillus acidophilus: b, bacillus subtilis: candida oviposition =1:1: 2.
4. A method for culturing indigenous lactobacillus is characterized by comprising the following steps:
(1) respectively taking lactobacillus strains, bacillus subtilis strains and saccharomycete strains, and adding a liquid culture medium into the strain mixture to obtain a compound strain liquid;
(2) adding brown sugar into the lactobacillus bacterial liquid obtained in the previous step for propagation, and controlling temperature and humidity;
(3) adding sodium silicate, water, brown sugar, wheat bran and jade bran into a mixer, mixing uniformly, fermenting with the composite bacteria liquid, and performing fermentation propagation;
(4) drying at low temperature by using a drying bed, sealing and storing for later use after fermentation is finished;
(5) adding the compound bacteria primary expanded strain, sodium silicate, water, brown sugar, wheat bran and jade bran into a mixer, uniformly mixing, controlling temperature and humidity, fermenting and propagating;
(6) the secondary expanded strain of the compound bacteria is put into a fermentation tank to be mixed with molasses urea water and stirred for fermentation
(7) And (4) pouring and sealing the fermented strains.
5. The complex EM flora for decontamination of aquaculture of claim 4, wherein the fermentation process in the third and fifth steps requires heap turnover for a total of 4 times per day.
6. The complex EM flora for decontamination of aquaculture of claim 4, wherein stirring is required during the fermentation process in the sixth step twice a day.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106986517A (en) * | 2017-03-28 | 2017-07-28 | 海南正强超越生化技术开发有限公司 | A kind of cultivation substrate modifier and preparation method thereof |
| CN109251874A (en) * | 2018-09-25 | 2019-01-22 | 厦门惠盈动物科技有限公司 | A kind of probiotics preparation and its preparation method and application |
| CN110282755A (en) * | 2019-07-10 | 2019-09-27 | 上海山恒生态科技股份有限公司 | A kind of formula and preparation method thereof of water remediation microbial inoculum |
| CN110577913A (en) * | 2019-09-29 | 2019-12-17 | 海南卓越生物有限公司 | Compound bacterium group formula for reducing ammonia nitrogen sub-salt and preparation method |
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- 2019-12-29 CN CN201911386517.8A patent/CN111019864A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106986517A (en) * | 2017-03-28 | 2017-07-28 | 海南正强超越生化技术开发有限公司 | A kind of cultivation substrate modifier and preparation method thereof |
| CN109251874A (en) * | 2018-09-25 | 2019-01-22 | 厦门惠盈动物科技有限公司 | A kind of probiotics preparation and its preparation method and application |
| CN110282755A (en) * | 2019-07-10 | 2019-09-27 | 上海山恒生态科技股份有限公司 | A kind of formula and preparation method thereof of water remediation microbial inoculum |
| CN110577913A (en) * | 2019-09-29 | 2019-12-17 | 海南卓越生物有限公司 | Compound bacterium group formula for reducing ammonia nitrogen sub-salt and preparation method |
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