CN110577913A - Compound bacterium group formula for reducing ammonia nitrogen sub-salt and preparation method - Google Patents
Compound bacterium group formula for reducing ammonia nitrogen sub-salt and preparation method Download PDFInfo
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- CN110577913A CN110577913A CN201910934818.3A CN201910934818A CN110577913A CN 110577913 A CN110577913 A CN 110577913A CN 201910934818 A CN201910934818 A CN 201910934818A CN 110577913 A CN110577913 A CN 110577913A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F2101/00—Nature of the contaminant
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- C02F2101/38—Organic compounds containing nitrogen
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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Abstract
The invention discloses a compound flora formula for reducing ammonia nitrogen sub-salt and a preparation method thereof, wherein the compound flora formula comprises denitrifying bacteria, nitrosomonas bacteria and nitrobacteria; also comprises a supplementary carrier culture medium of denitrifying bacteria, nitrosomonas and nitrobacter. The invention takes the reduction of indexes such as nitrite, ammonia nitrogen and the like in the aquaculture water body as a main research and development direction, adopts advanced microbial engineering technology, scientifically and reasonably configures a carrier culture medium, effectively synthesizes the advantages and features of strains such as denitrifying bacteria, nitrosomonas bacteria, nitrobacillus and the like, fully exerts the synergistic effect of each strain and achieves the effect of synchronously and obviously degrading the ammonia nitrogen and the nitrite.
Description
Technical Field
the invention relates to the technical field of microbial fermentation, in particular to a formula and a preparation method of a compound flora for reducing ammonia nitrogen sub-salt.
background
Residual baits left by a large amount of baits thrown in a water body, accumulation of a large amount of excreta of aquatic animals in the water body, continuous increase of culture density for pursuing income, large water body load caused by a disinfectant and drug residues used regularly and the like in aquaculture of aquatic products (freshwater shrimps, river shrimps, prawns, metapenaeus ensis, lutea, lobsters, crabs, sea cucumbers, frogs, loaches, eels, turtles, finless eels and sea fresh water fishes), and physical and chemical indexes of the culture water body are damaged, namely water quality deterioration becomes turbid, pond water turns green, black, reddens and even becomes bad and smelly. Leading to the massive production of harmful substances such as ammonia nitrogen and nitrite in water, particularly leading to the overproof of nitrite ammonia nitrogen, and seriously leading to the death of aquatic organisms caused by poisoning, therefore, microorganisms need to be put into the water body to realize the purification of the water body.
Disclosure of Invention
aiming at the problems, the invention provides a formula and a preparation method of a composite flora for reducing ammonia nitrogen sub-salt, which can effectively solve the problems in the background art.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows: a compound flora formula for reducing ammonia nitrogen sub-salt, wherein the compound flora formula comprises denitrifying bacteria, nitrosomonas and nitrobacter; also comprises a supplementary carrier culture medium of denitrifying bacteria, nitrosomonas and nitrobacter;
the culture medium of the denitrifying bacteria comprises the following components in percentage by weight: 30 g of sodium potassium tartrate, 1g K2HPO4, 4 g of KNO2, 0.9g of MgSO4 & 7H2O, pH 7.8, 2000mL of distilled water, and 10% agar added to the solid medium;
Nitrifying bacteria culture medium: 2g of NaNO2, 0.9g of MgSO4 & 7H2O, 0.3g K2 of HPO4, 0.06g of MnSO4 & 4H2O, 3g of Na2CO3, 0.7g of NaH2PH4, the PH value is natural, distilled water 2000m L, and 10% agar is added into a solid culture medium;
The nitrite-degrading bacteria culture medium comprises 10g of glucose, 3g of NaCl, 5g of NaNO2, 0.7g of MgSO4, 8g of KH2PO4 and 2g K2HPO4, wherein the pH value is natural, 3000mL of distilled water is added, and 10% agar is added into the solid culture medium;
The growth medium comprises 20g of peptone, 6g of yeast powder, 6g of starch, 0.8g of MgSO4, 3g of KH2PO4, 4 g of NaH2PH4, pH 7.4 and 2000mL of distilled water.
Further, the ratio of the strains to the raw materials is as follows: 90% of jade bran and 10% of compound bacterium liquid.
further, the quantity ratio of denitrifying bacteria, nitrosomonas and nitrobacteria is 2: 1:2.
a preparation method of composite flora for reducing ammonia nitrogen sub-salt comprises the following steps:
(1) Respectively taking denitrifying bacteria, nitrosomonas species and nitrobacter species, and adding a growth medium to prepare a compound bacteria liquid;
(2) Selecting high-quality corn bran, crushing, uniformly mixing, adding a compound bacterium solution, and fermenting together;
(3) carrying out propagation on the fermented compound bacterium solution, and carrying out temperature-controlled culture;
(4) Drying at low temperature;
(5) and (5) subpackaging.
further, in the step (1), a proper amount of brown sugar is added into the composite bacteria solution for propagation.
the invention has the beneficial effects that: the invention takes the reduction of indexes such as nitrite, ammonia nitrogen and the like in the aquaculture water body as a main research and development direction, adopts advanced microbial engineering technology, scientifically and reasonably configures a carrier culture medium, effectively synthesizes the advantages and features of strains such as denitrifying bacteria, nitrosomonas bacteria, nitrobacillus and the like, fully exerts the synergistic effect of each strain and achieves the effect of synchronously and obviously degrading the ammonia nitrogen and the nitrite.
drawings
FIG. 1 is a flow chart of the production of the product of imidocarb-ofloxacin of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
example (b):
a compound flora formula for reducing ammonia nitrogen sub-salt, wherein the compound flora formula comprises denitrifying bacteria, nitrosomonas and nitrobacter; also comprises a supplementary carrier culture medium of denitrifying bacteria, nitrosomonas and nitrobacter;
the culture medium of the denitrifying bacteria comprises the following components in percentage by weight: 30 g of sodium potassium tartrate, 1g K2HPO4, 4 g of KNO2, 0.9g of MgSO4 & 7H2O, pH 7.8, 2000mL of distilled water, and 10% agar added to the solid medium;
nitrifying bacteria culture medium: 2g of NaNO2, 0.9g of MgSO4 & 7H2O, 0.3g K2 of HPO4, 0.06g of MnSO4 & 4H2O, 3g of Na2CO3, 0.7g of NaH2PH4, the PH value is natural, distilled water 2000m L, and 10% agar is added into a solid culture medium;
The nitrite-degrading bacteria culture medium comprises 10g of glucose, 3g of NaCl, 5g of NaNO2, 0.7g of MgSO4, 8g of KH2PO4 and 2g K2HPO4, wherein the pH value is natural, 3000mL of distilled water is added, and 10% agar is added into the solid culture medium;
the growth medium comprises 20g of peptone, 6g of yeast powder, 6g of starch, 0.8g of MgSO4, 3g of KH2PO4, 4 g of NaH2PH4, pH 7.4 and 2000mL of distilled water.
Further, the ratio of the strains to the raw materials is as follows: 90% of jade bran and 10% of compound bacterium liquid.
further, the quantity ratio of denitrifying bacteria, nitrosomonas and nitrobacteria is 2: 1:2.
A preparation method of composite flora for reducing ammonia nitrogen sub-salt comprises the following steps:
(1) respectively taking denitrifying strains, nitrosomonas species and nitrobacter species, adding a growth medium to prepare a compound bacteria liquid, and adding a proper amount of brown sugar into the compound bacteria liquid for propagation;
(2) selecting high-quality corn bran, crushing, uniformly mixing, adding a compound bacterium solution, and fermenting together;
(3) Carrying out propagation on the fermented compound bacterium solution, and carrying out temperature-controlled culture;
(4) Drying at low temperature;
(5) And (5) subpackaging.
the corresponding culture mode of the composite bacteria in the technical scheme can refer to a culture method of nitrite bacteria and a treatment method of wastewater containing ammonia nitrogen in a comparison document 1CN200710158375.0, and the ammonia nitrogen concentration and the pH value of the substrate are increased in the enrichment process, so that free NH3 with higher concentration in the substrate is maintained, and the aims of eliminating the nitrate bacteria and enriching the nitrite bacteria are gradually achieved, and the nitrite bacteria is obtained. And then, taking the obtained nitrite flora as a seed, and utilizing self-prepared ammonia nitrogen wastewater or high ammonia nitrogen wastewater as a culture solution for amplification culture, wherein the nitrite flora subjected to amplification culture is inoculated in a biochemical treatment tank for improving the efficiency of biochemical treatment for deamination and denitrification, or is directly used for deamination and denitrification of the ammonia nitrogen wastewater, most preferably used for accumulating nitrite nitrogen in an aerobic nitrification stage of a short-cut nitrification and denitrification process, and directly used as an electron acceptor for denitrification. The method has simple process and prominent effect of treating the wastewater containing ammonia nitrogen. And reference 2N201810441790.5 a method for high-density anaerobic ammonium oxidation bacteria enrichment in a sewage denitrification treatment system, the method comprising the steps of: 1) collecting nitrified sludge containing anaerobic ammonium oxidation bacteria flora, and preparing an activated sludge solution; inoculating the strain in an enrichment medium, and performing nitration domestication until the conversion rate of ammonia nitrogen is not less than 80%; 2) inoculating the acclimated sludge into a reactor, adding macroporous polyurethane filler and substrate ammonia nitrogen and nitrite nitrogen, and starting an anaerobic ammonia oxidation reactor at low substrate concentration; 3) increasing the addition of the matrix, and starting the anaerobic ammonia oxidation reactor at medium and high matrix concentration; 4) and continuously increasing the addition of the matrix to enrich the high-density anaerobic ammonium oxidation bacteria. The macroporous polyurethane filler adopted by the invention can provide a micro-aerobic environment for the anammox bacteria, can adapt to the agglomeration growth characteristic of the anammox bacteria, is beneficial to eliminating AOB, NOB and other bacteria, and can obtain the anammox bacteria with higher purity and abundance. So as to realize the optimization of the strains in the culture process.
The use mode of the compound flora for reducing the ammonia nitrogen and the nitrite in the water body can refer to CN201910063184.9 in the comparison document 3 to degrade the ammonia nitrogen and the nitrite in the water body, and the application thereof
Based on the above, the invention has the advantages that the advanced microbial engineering technology is adopted, the carrier culture medium is scientifically and reasonably configured, the advantages and the characteristics of strains such as denitrifying bacteria, nitrosomonas bacteria, nitrobacillus and the like are effectively integrated, the synergistic effect of each strain is fully exerted, and the effect of synchronously and obviously degrading ammonia nitrogen and nitrite is achieved.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (5)
1. the formula of the compound flora for reducing the ammonia nitrogen nitrite is characterized in that: the formula of the composite flora comprises denitrifying bacteria, nitrosomonas and nitrobacter; also comprises a supplementary carrier culture medium of denitrifying bacteria, nitrosomonas and nitrobacter;
The culture medium of the denitrifying bacteria comprises the following components in percentage by weight: 30 g of sodium potassium tartrate, 1g K2HPO4, 4 g of KNO2, 0.9g of MgSO4 & 7H2O, pH 7.8, 2000mL of distilled water, and 10% agar added to the solid medium;
nitrifying bacteria culture medium: 2g of NaNO2, 0.9g of MgSO4 & 7H2O, 0.3g K2 of HPO4, 0.06g of MnSO4 & 4H2O, 3g of Na2CO3, 0.7g of NaH2PH4, the PH value is natural, distilled water 2000m L, and 10% agar is added into a solid culture medium;
the nitrite-degrading bacteria culture medium comprises 10g of glucose, 3g of NaCl, 5g of NaNO2, 0.7g of MgSO4, 8g of KH2PO4 and 2g K2HPO4, wherein the pH value is natural, 3000mL of distilled water is added, and 10% agar is added into the solid culture medium;
the growth medium comprises 20g of peptone, 6g of yeast powder, 6g of starch, 0.8g of MgSO4, 3g of KH2PO4, 4 g of NaH2PH4, pH 7.4 and 2000mL of distilled water.
2. The formulation of claim 1, wherein the ratio of bacteria to raw materials is: 90% of jade bran and 10% of compound bacterium liquid.
3. The formula of kanamycin antibody prepared from Chinese herbal pieces as claimed in claim 1, wherein the number ratio of denitrifying bacteria, nitrosomonas bacteria and nitrobacteria is 2: 1:2.
4. the preparation method of the compound bacteria for reducing the ammonia nitrogen nitrite is characterized by comprising the following steps:
(1) respectively taking denitrifying bacteria, nitrosomonas species and nitrobacter species, and adding a growth medium to prepare a compound bacteria liquid;
(2) Selecting high-quality corn bran, crushing, uniformly mixing, adding a compound bacterium solution, and fermenting together;
(3) Carrying out propagation on the fermented compound bacterium solution, and carrying out temperature-controlled culture;
(4) drying at low temperature;
(5) And (5) subpackaging.
5. The method for preparing composite flora for reducing ammonia nitrogen sub-salt according to claim 4, wherein a proper amount of brown sugar is added into the composite flora solution in the step (1) for propagation.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111019864A (en) * | 2019-12-29 | 2020-04-17 | 海南卓越生物有限公司 | Preparation method of composite EM (effective microorganisms) flora for decontamination of aquaculture |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101831392A (en) * | 2009-03-10 | 2010-09-15 | 中国科学院成都生物研究所 | Autotrophic and allotrophic symbiosis ammonia oxidation bacterial agent as well as culture method and application thereof |
| CN107603909A (en) * | 2017-09-30 | 2018-01-19 | 河北天泓环保科技有限公司 | Sewage treatment microbial inoculant and its application method |
| CN109205795A (en) * | 2017-12-21 | 2019-01-15 | 向勇 | A kind of compound nitrifier and its production method |
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- 2019-09-29 CN CN201910934818.3A patent/CN110577913A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101831392A (en) * | 2009-03-10 | 2010-09-15 | 中国科学院成都生物研究所 | Autotrophic and allotrophic symbiosis ammonia oxidation bacterial agent as well as culture method and application thereof |
| CN107603909A (en) * | 2017-09-30 | 2018-01-19 | 河北天泓环保科技有限公司 | Sewage treatment microbial inoculant and its application method |
| CN109205795A (en) * | 2017-12-21 | 2019-01-15 | 向勇 | A kind of compound nitrifier and its production method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111019864A (en) * | 2019-12-29 | 2020-04-17 | 海南卓越生物有限公司 | Preparation method of composite EM (effective microorganisms) flora for decontamination of aquaculture |
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