CN104745507B - One plant of low-temperature aerobic denitrifying bacterium and its application - Google Patents
One plant of low-temperature aerobic denitrifying bacterium and its application Download PDFInfo
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- 241000894006 Bacteria Species 0.000 title claims abstract description 50
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 27
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000002351 wastewater Substances 0.000 claims description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 50
- 229910002651 NO3 Inorganic materials 0.000 abstract description 42
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 abstract description 42
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 25
- 230000012010 growth Effects 0.000 abstract description 13
- 229910052799 carbon Inorganic materials 0.000 abstract description 7
- 238000012851 eutrophication Methods 0.000 abstract description 6
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 abstract description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 4
- 230000001580 bacterial effect Effects 0.000 abstract description 3
- 239000001963 growth medium Substances 0.000 description 28
- 230000015556 catabolic process Effects 0.000 description 8
- 238000006731 degradation reaction Methods 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 7
- 241001669635 Pseudomonas brassicacearum subsp. brassicacearum Species 0.000 description 7
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 6
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 5
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 5
- 239000012153 distilled water Substances 0.000 description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 235000013619 trace mineral Nutrition 0.000 description 4
- 239000011573 trace mineral Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 238000011953 bioanalysis Methods 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000006396 nitration reaction Methods 0.000 description 3
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 239000010865 sewage Substances 0.000 description 3
- 239000001632 sodium acetate Substances 0.000 description 3
- 235000017281 sodium acetate Nutrition 0.000 description 3
- 229910019629 (NH4)6Mo7O2·4H2O Inorganic materials 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
- 229910021380 Manganese Chloride Inorganic materials 0.000 description 2
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 description 2
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 2
- 102000004316 Oxidoreductases Human genes 0.000 description 2
- 108090000854 Oxidoreductases Proteins 0.000 description 2
- 241000226031 Pseudomonas brassicacearum Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 2
- 229910052564 epsomite Inorganic materials 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000011565 manganese chloride Substances 0.000 description 2
- 229910052603 melanterite Inorganic materials 0.000 description 2
- 229910017604 nitric acid Inorganic materials 0.000 description 2
- 150000002978 peroxides Chemical class 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 description 1
- 229910016374 CuSO45H2O Inorganic materials 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 241000108664 Nitrobacteria Species 0.000 description 1
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 229910052927 chalcanthite Inorganic materials 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 235000002867 manganese chloride Nutrition 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- AHYBQDWHRPRCKD-UHFFFAOYSA-N potassium nitric acid nitrate Chemical compound [K+].O[N+]([O-])=O.[O-][N+]([O-])=O AHYBQDWHRPRCKD-UHFFFAOYSA-N 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 239000011686 zinc sulphate Substances 0.000 description 1
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/38—Pseudomonas
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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Abstract
The invention discloses one plant of low-temperature aerobic denitrifying bacterium and its application.One plant of low-temperature aerobic denitrifying bacteriumPseudomonas brassicacearum Subsp.brassicacearum BW0806, deposit number are：CCTCC NO:M 2015094.The bacterial strain can be in 10 15 DEG C of growths, and realize denitrification under the conditions of aerobic, low ratio of carbon to ammonium.At 15 DEG C, initial nitrate concentration is under 30mg/L aerobic condition（DO>2mg/L）, the clearance of the bacterium 72h nitrate and total nitrogen reaches 100%.The low-temperature aerobic denitrification bacterial strain is applied to carry out the denitrification denitrogenation processing of eutrophication water and nitrogenous effluent under low temperature, aerobic condition.
Description
Technical field
The invention belongs to environmental microorganism, field of environment engineering technology, is related to one plant of low-temperature aerobic denitrifying bacterium
Pseudomona s brassicacearum subsp.brassicacearum BW0806 and its in low temperature eutrophication water
And the denitrogenation application in nitrogenous effluent.
Background technology
Nitrogen in water body, which is built up, can cause body eutrophication phenomenon, seriously endanger ecology erroneous zone.Water body
The method of denitrogenation mainly has Physical, chemical method and bioanalysis.Compared to physico-chemical process, bioanalysis has processing cost
The advantages that low, non-secondary pollution, so typically carrying out denitrogenation of waste water using bioanalysis.
Waste water control under low temperature environment is always the focus and difficult point in water harnessing field.Low temperature has for thalline denitrification
Significant inhibitory action, there are some researches show temperature often reduces by 1 DEG C, denitrification rate then reduces by 9%, U.Welander etc. and also studied
The runnability for the denitrification process known clearly at 3~20 DEG C of low temperature, the denitrification rate of reactor is only at 15 DEG C at 3 DEG C
55%.Current research more suggests improving the removal of nitrogen at low ambient temperatures using pyschrophile.Therefore, efficient low temperature is filtered out
Denitrifying bacteria, it is more scientific reasonably to utilize pyschrophile resource, strengthen it in terms of low-temperature wastewater treatment and aquatic ecological restoration
Using being very necessary.
In recent years, aerobic denitrification denitrogenation technology turns into the new developing direction of domestic and international denitrogenation of waste water, and more traditional anaerobism is anti-
Nitrification denitrogenation has inborn advantage.At present, the report about low-temperature aerobic denitrifying bacterium is less, Chinese patent literature number
CN102690 765A date of publication 2012.9.26, disclose one plant of cold-resistant pseudomonad Den-03 and its application, the low temperature-resistant strains
Total nitrogen can be preferably removed under cryogenic.What is filtered out from nature is capable of the low of efficient removal nitrate from waste water
Processing of the warm denitrifying bacterium to winter eutrophic water body and nitrogenous effluent has good actual application value.
The content of the invention
It is an object of the present invention to the problem of being directed to nitrate sewage water denitrification difficulty under low temperature, aerobic environment, there is provided one plant
Low-temperature aerobic denitrifying bacteria, the bacterium have delivered to China typical culture collection center on March 9th, 2015 and have carried out preservation,
Classification And Nomenclature：Pseudomonas brassicacearum subsp.brassicacearum BW0806, deposit number are：
CCT CC NO:M 2015094, address：Wuhan, China Wuhan University.
It is another object of the present invention to provide one plant of low-temperature aerobic denitrifying bacteria low temperature eutrophic water
The application in biological reinforced denitrogenation is carried out in body or nitrogenous effluent.
For achieving the above object, technical scheme is as follows：
Applicant breathes out from Qinghai cloth and sample is gathered in river body, separates single bacterium colony, cultivates, pass through in denitrification culture medium
Nitrate, nitrite, the content of total nitrogen in culture medium are determined, is finally obtained one plant of low-temperature aerobic denitrifying bacterium, the bacterium
China typical culture collection center has been delivered on March 9th, 2015 and has carried out preservation, Classification And Nomenclature：Pseudomonas
Brassica cearum subsp.brassicacearum BW0806, deposit number are：CCTCC NO:M 2015094, ground
Location：Wuhan, China Wuhan University.
Described low-temperature aerobic denitrifying bacterium is Gram-negative bacteria, and Adaptable growth temperature is 10~15 DEG C.In LB solids
Cultivated in culture medium, its colonial morphology is：Surface is smooth, yellow-white.Physiological and biochemical property is：It is aerobic, oxidase positive, peroxide
Change hydrogen enzyme positive, there is nitrate reduction ability.
Described LB solid mediums form：Tryptone 10g, NaCl 10g, yeast extract 5g, agar powder 15g,
It is dissolved in 1000mL distilled water, adjusts PH7.0.
Described denitrification culture medium forms：KNO30.5g；KH2PO41g；MgSO4·7H2O 0.1g；NH4Cl
0.2g；Sodium acetate 4g, trace element solution 2ml are dissolved in 1000mL distilled water, adjust PH7.2.Trace element solution forms：E
DTA 50g；CaCl25.5g；ZnSO42.2g；MnCl2·4H2O 5.06g；FeSO4·7H2O 5g；(NH4)6Mo7O2·
4H2O 1.1g；CuSO45H2O 1.57g, CoCl26H2O 1.61g, are dissolved in 1000mL distilled water；PH7.2.
Low-temperature aerobic denitrifying bacterium provided by the invention is when temperature is 15 DEG C, in initial NO3-N is the anti-of 80mg/L
Cultivated in nitrification culture medium, the degradation rate of nitrate is 100% after 8d, the degradation rate about 97% of total nitrogen.10 DEG C of cultivation temperature
Under in denitrification culture medium after 8d nitrate degradation rate be 100%, the degradation rate about 60% of total nitrogen.In 30 DEG C of growths and
Denitrification situation belongs to thermophilic pyschrophile not as good as cryogenic conditions.
Low-temperature aerobic denitrifying bacterium in the present invention adapts to low ratio of carbon to ammonium (C/N=2~10), particularly in C/N=5,15
DEG C, nitrate initial concentration be 80mg/L condition of culture under, nitrate can be completely degraded in 3d, total nitrogen content degrade about
95%.
Low-temperature aerobic denitrifying bacterium in the present invention adapts to aerobic environment, is 80mg/L in 15 DEG C, nitrate initial concentration,
Dissolved oxygen amount>Under 2mg/L, C/N=5 condition of culture, nitrate can be completely degraded in 4d, total nitrogen content degraded about 82%,
Can preferably it be grown compared with anaerobic environment and anti-nitration reaction.
Low-temperature aerobic denitrifying bacterium in the present invention is in 15 DEG C, C/N=5 aerobic condition (dissolved oxygen amount>Can under 2mg/L)
The nitrate of load high concentration, nitrate initial concentration are that 72h nitrate removals rate is about in 300mg/L denitrification culture medium
For 75%.72h can remove 100% nitric acid in the middle concentration denitrification culture medium that nitrate initial concentration is 160mg/L
Salt.Nitrate initial concentration be 30mg/L low concentration denitrification culture medium in 72h can remove 100% nitrate and
Total nitrogen content.
A kind of low-temperature aerobic denitrifying bacterium carries out biological reinforced denitrogenation in the eutrophication water or nitrogenous effluent of low temperature
In application, low-temperature aerobic denitrifying bacterium BW0806 is cultivated to increased logarithmic phase, with 104~106Cfu/mL throwing bacterium amount input
In eutrophic water, nitrate, total nitrogen content and COD in the eutrophic water that can effectively degrade.
In above-described application, it is preferred that manually adjust the environment in water body to C/N=2~10.
Compared with prior art, the present invention has advantages below：
(1) in 15 DEG C of low temperature, initial nitrate concentration is low-temperature aerobic denitrifying bacterium under 30mg/L aerobic condition
Can be degraded in Pseudo monas brassicacearum subsp.brassicacearum BW080672h 100% nitre
Hydrochlorate and total nitrogen content, reach the efficient removal of nitrate and total nitrogen under cryogenic conditions.
(2) the low-temperature aerobic denitrifying bacterium in the present invention belongs to thermophilic pyschrophile, adapts to 10~15 DEG C of growing environment, is the winter
The season denitrogenation of rivers and lakes, sewage effluent provides new thinking, and broken biological method low temperature administers the limitation of sewage.
(3) present invention in low-temperature aerobic denitrifying bacterium can under the condition of culture of low ratio of carbon to ammonium amount reproduction, for dirt
Water harnessing reduces cost.
(4) the low-temperature aerobic denitrifying bacterium in the present invention adapts to aerobic condition, have applied to eutrophication water and
Combine the potentiality that nitrobacteria synchronizes nitrification-denitrification in aerobic treatment tank.
(5) the low-temperature aerobic denitrifying bacterium in the present invention is applied in eutrophic water, can be effective under cryogenic
Denitrogenation and degraded COD.
Embodiment
This explanation is further detailed with reference to embodiments, but use range of the present invention is not limited only to this.
Embodiment 1：
The acquisition of low-temperature aerobic denitrifying bacterium：
A. breathed out from Qinghai cloth and sample is gathered in river body, separated single bacterium colony and identify.
B. by the bacterial strain that step A is separated in 15 DEG C of beef extract-peptone fluid nutrient medium cultures, obtaining can at 15 DEG C
The pyschrophile of growth.
C. pyschrophile step B filtered out is inoculated in denitrification culture medium, 15 DEG C of cultures, by determining in culture medium
Nitrate, nitrite, the content of total nitrogen, to judge to screen efficient low temperature denitrifying bacterium.
One plant of low temperature denitrifying bacterium is finally obtained, the bacterium has delivered to Chinese Typical Representative culture guarantor on March 9th, 2015
Tibetan center carries out preservation, Classification And Nomenclature：Pseudomonas brassicacearum subsp.brassicacearum
BW0806, deposit number are：CCTCC NO:M 2015094, address：Wuhan, China Wuhan University.
Described low-temperature aerobic denitrifying bacterium is Gram-negative bacteria, and Adaptable growth temperature is 10~15 DEG C, and culture medium is
Denitrification culture medium.Its colonial morphology is：Surface is smooth, yellow-white.Physiological and biochemical property is：It is aerobic, oxidase positive, peroxide
Change hydrogen enzyme positive, there is nitrate reduction ability.
Denitrification culture medium forms：KNO30.5g；KH2PO41g；MgSO4·7H2O 0.1g；NH4Cl 0.2g；Acetic acid
Sodium 4g, trace element solution 2ml are dissolved in 1000mL distilled water, adjust pH7.2.Trace element solution forms：EDTA 50g；
CaCl25.5g；ZnSO42.2g；MnCl2·4H2O 5.06g；FeSO4·7H2O 5g；(NH4)6Mo7O2·4H2O 1.1g；
CuSO4·5H2O 1.57g,CoCl2·6H2O 1.61g are dissolved in 1000mL distilled water；pH7.2.
Embodiment 2:
Pseudomonas brassicacearum subsp.Brassicacearum BW0806 growth preference temperature：
Low-temperature aerobic denitrifying bacterium culture to the increased logarithmic phase is inoculated in denitrification culture medium and adjusts initial OD600
About 0.05,120rpm sealings Shaking culture, nitrate, nitrite, total nitrogen in culture medium are determined per 24h at different temperature
Content and OD600 values (table 1).The parameter value of measure described above (removes OD600) it is to centrifuge the correlation after thalline in supernatant
Value.
Table 1
As a result show the low-temperature aerobic denitrifying bacterium adapt to 15 DEG C growth and anti-nitration reaction, in the drop of eight days nitrate
Solution rate is 100%, the degradation rate about 97% of total nitrogen.Under 10 DEG C of cultivation temperature in denitrification culture medium, in the 8th day nitric acid
The degradation rate of salt is 100%, the degradation rate about 60% of total nitrogen.In 30 DEG C of growths and denitrification situation not as good as cryogenic conditions, category
In thermophilic pyschrophile.
Embodiment 3：
The Pseudomonas brassicacearum subsp.brassicacearumBW0806 suitable carbon nitrogen of growth
Than：
Under the conditions of 15 DEG C, the low-temperature aerobic denitrifying bacterium culture is inoculated in different carbon-nitrogen ratios to increased logarithmic phase respectively
Initial OD 600 about 0.05 is adjusted in the denitrification culture medium of (carbon-nitrogen ratio is adjusted by adding the sodium acetate of different quality),
120rpm seals Shaking culture, nitrate, nitrite, total nitrogen content and OD600 values per 24h in measure culture medium.More than
The parameter value of the measure (removes OD600) it is to centrifuge the correlation after thalline in supernatant.
Table 2
Testing result shows that the low-temperature aerobic denitrifying bacterium adapts to growth and anti-nitre in low ratio of carbon to ammonium (2~10) culture medium
Change reaction.It is 100% in the clearance of the 3rd day nitrate in C/N=5, the clearance of total nitrogen is 95%.
Embodiment 4：
The Pseudomonas brassicacearum subsp.brassicacearum BW0806 suitable dissolved oxygen of growth
Amount：
Under the conditions of 15 DEG C, carbon-nitrogen ratio is under 5 condition of culture, by the low-temperature aerobic denitrifying bacterium in the anti-of different dissolved oxygen amounts
Nitrify culture medium in breathe freely culture, adjust initial OD 600 about 0.05, per 24h measure culture medium in nitrate, nitrite,
Total nitrogen content and OD600 values.The parameter value of measure described above (removes OD600) it is to centrifuge the correlation after thalline in supernatant.
Table 3
Testing result shows that the bacterium is adapted in aerobic (DO>Growth and anti-nitration reaction in condition of culture 2mg/L), wherein
It can be completely degraded in the 4th day nitrate, total nitrogen content degraded about 82%.Under anaerobic condition the denitrification situation of the bacterium and
Growing state is poor compared with aerobic condition of culture.
Embodiment 5：
The Pseudomonas brassicacearum subsp.brassicacearumBW0806 suitable nitrate of growth
Concentration：
Under the conditions of 15 DEG C, carbon-nitrogen ratio 5, aerobic (DO>Under condition of culture 2mg/L), by the low-temperature aerobic denitrifying bacterium
Be inoculated in respectively low (30mg/L), in (160mg/L), high (300mg/L) concentration nitrate denitrification culture medium in adjust just
Beginning OD600 about 0.05,120rpm seal Shaking culture, per 12h measure culture medium in nitrate, nitrite, total nitrogen content and
OD600 values, the parameter value of measure described above (remove OD600) it is to centrifuge the correlation after thalline in supernatant.
Table 4
As a result show that bacterium denitrification ability under the condition of culture of 30mg/L protonitrate concentration is best, 72h can degrade
100% nitrate.Nitrate removal ability in centering concentration nitrate culture-medium (nitrate containing 160mg/L) is shown
Color, it is capable of the nitrate of degradable 160mg/L concentration.To the nitre of high concentration nitrate culture medium (nitrate containing 300mg/L)
Hydrochlorate degradation rate is about 75%.Low-temperature aerobic denitrifying bacterium all tables in the denitrification culture medium of basic, normal, high concentration nitrate
Outstanding growing state is revealed.
Embodiment 6：
Eutrophication water sample is gathered, determines PH, temperature, nitrate nitrogen, nitrite nitrogen, ammonia nitrogen, total nitrogen and COD in water sample.Add
Enter potassium nitrate nitrate content about 10mg/L into water sample, it is therefore an objective to its serious low temperature Denitrification Characteristics, add sodium acetate regulation
C/N=5 (mass ratio) in water sample.By Pseudomonas brassicacearum subsp.Brassicacearum BW080
6 cultivate to increased logarithmic phase in denitrification culture medium, with 104~106Cfu/mL throwing bacterium amount puts into initial nitrate concentration about
10mg/L, initial total nitrogen concentration about 18mg/L eutrophic water in, about 10 DEG C of temperature, ammonia-nitrogen content after pH value about 8.0,12d
For 0, nitrate, the content of total nitrogen and COD reduce about 80%.
Claims (5)
1. one plant of low-temperature aerobic denitrifying bacterium, it is characterised in that：The Classification And Nomenclature of described low-temperature aerobic denitrifying bacterium isPseudomonas brassicacearum subsp. brassicacearumBW0806, deposit number are：CCTCC NO:
M 2015094。
2. application of the low-temperature aerobic denitrifying bacterium in eutrophic water body denitrogenation described in claim 1.
3. application of the low-temperature aerobic denitrifying bacterium in nitrogenous effluent denitrogenation described in claim 1.
4. the application according to Claims 2 or 3, the dosage of described low-temperature aerobic denitrifying bacterium is 104~106cfu/
mL。
5. the application according to Claims 2 or 3, described water body or waste water through manually adjust for：C/N=2~10.
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| 一株耐盐好氧反硝化细菌的分离鉴定及生长特性研究;吐尔逊阿依•吾甫尔等;《新疆农业科学》;20101231;第47卷(第11期);2216-2220 * |
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