CN104745507A - Low-temperature aerobic denitrifying bacterium and application thereof - Google Patents
Low-temperature aerobic denitrifying bacterium and application thereof Download PDFInfo
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- CN104745507A CN104745507A CN201510124201.7A CN201510124201A CN104745507A CN 104745507 A CN104745507 A CN 104745507A CN 201510124201 A CN201510124201 A CN 201510124201A CN 104745507 A CN104745507 A CN 104745507A
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- 241000894006 Bacteria Species 0.000 title claims abstract description 49
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000002351 wastewater Substances 0.000 claims abstract description 7
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims abstract description 6
- 241000589516 Pseudomonas Species 0.000 claims abstract description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 52
- 229910002651 NO3 Inorganic materials 0.000 abstract description 48
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 abstract description 48
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 26
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 abstract description 6
- 238000004321 preservation Methods 0.000 abstract description 4
- 230000001580 bacterial effect Effects 0.000 abstract description 3
- 230000012010 growth Effects 0.000 description 11
- 230000015556 catabolic process Effects 0.000 description 9
- 238000006731 degradation reaction Methods 0.000 description 9
- 241001669635 Pseudomonas brassicacearum subsp. brassicacearum Species 0.000 description 8
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 6
- 229910052799 carbon Inorganic materials 0.000 description 6
- 238000012851 eutrophication Methods 0.000 description 6
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 5
- 239000012153 distilled water Substances 0.000 description 5
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000006396 nitration reaction Methods 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 239000001632 sodium acetate Substances 0.000 description 4
- 235000017281 sodium acetate Nutrition 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 235000013619 trace mineral Nutrition 0.000 description 4
- 239000011573 trace mineral Substances 0.000 description 4
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 230000031018 biological processes and functions Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000010865 sewage Substances 0.000 description 3
- 238000012807 shake-flask culturing Methods 0.000 description 3
- 102000016938 Catalase Human genes 0.000 description 2
- 108010053835 Catalase Proteins 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 102000004316 Oxidoreductases Human genes 0.000 description 2
- 108090000854 Oxidoreductases Proteins 0.000 description 2
- 241000226031 Pseudomonas brassicacearum Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 1
- 229910016374 CuSO45H2O Inorganic materials 0.000 description 1
- 241000108664 Nitrobacteria Species 0.000 description 1
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- -1 at C/N=5 Chemical compound 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 108010046845 tryptones Proteins 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/38—Pseudomonas
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
Abstract
The invention discloses a low-temperature aerobic denitrifying bacterium and an application thereof. The low-temperature aerobic denitrifying bacterium pseudomonas brassicacerarum subsp.brassicacearum BW0806 has a preservation number of CCTCC NO:M2015094. The bacterial strain can grow at 10-15 DEG C, and can realize denitrifying under an aerobic condition with a low carbon-nitrogen ratio. At 15 DEG C, the removal rate for 72h nitrate and total nitrogen of the bacterium under the aerobic condition with initial nitrate concentration of 30mg/L (DO higher than 2mg/L) at 15 DEG C is 100%. The low-temperature aerobic denitrifying bacterial strain is suitable for denitrifying and denitriding a eutrophic body of water and nitrogen-containing wastewater under the aerobic condition at a low temperature.
Description
Technical field
The invention belongs to environmental microorganism, field of environment engineering technology, relate to a strain low-temperature aerobic denitrifying bacteria Pseudomonas brassicacearum subsp.brassicacearum BW0806 and the application of the denitrogenation in low temperature eutrophication water and nitrogenous effluent thereof.
Background technology
Nitrogen in water body excessively accumulation can cause body eutrophication phenomenon, serious harm ecology erroneous zone.The method of water body denitrification mainly contains Physical, chemical method and biological process.Compared to physico-chemical process, biological process has the advantages such as processing cost is low, non-secondary pollution, adopts biological process to carry out denitrogenation of waste water so general.
Waste water control under low temperature environment is focus and the difficult point in water harnessing field always.Low temperature has significant restraining effect for thalline denitrification, there are some researches show that temperature often reduces by 1 DEG C, denitrification rate then reduces by 9%, U.Welander etc. also studied the operating performance of the denitrification process at low temperature 3 ~ 20 DEG C, and at 3 DEG C, the denitrification rate of reactor is only 55% at 15 DEG C.Current research is more advised using psychrophile to improve the removal of nitrogen at low ambient temperatures.Therefore, filter out efficient low temperature denitrifying bacterium, more scientificly reasonably utilize psychrophile resource, it is very necessary for strengthening its application in low-temperature wastewater treatment and aquatic ecological restoration.
In recent years, aerobic denitrification denitride technology becomes the new developing direction of domestic and international denitrogenation of waste water, and more traditional anaerobic denitrifying denitrogenation has inborn advantage.At present, the report of associated cryogenic aerobic denitrifying bacteria is less, Chinese patent literature CN102690765A date of publication 2012.9.26, and disclose the cold-resistant pseudomonas Den-03 of a strain and application thereof, this low temperature-resistant strains can remove total nitrogen under cryogenic preferably.From occurring in nature filter out can efficient removal nitrate from waste water low temperature denitrifying bacteria to winter eutrophic water body and the process of nitrogenous effluent have good actual application value.
Summary of the invention
The object of the invention is to, for the problem of nitrate sewage water denitrification difficulty under low temperature, aerobic environment, one strain low-temperature aerobic denitrifying bacterium is provided, this bacterium is delivered to China typical culture collection center on March 9th, 2015 and carries out preservation, Classification And Nomenclature: Pseudomonas brassicacearum subsp.brassicacearum BW0806, deposit number is: CCTCC NO:M 2015094, address: Wuhan, China Wuhan University.
Another object of the present invention there are provided the application that a strain low-temperature aerobic denitrifying bacterium carries out in biological reinforced denitrogenation in the eutrophication water or nitrogenous effluent of low temperature.
For achieving the above object, technical scheme of the present invention is as follows:
Applicant breathes out collected specimens river body from Qinghai cloth, be separated single bacterium colony, cultivate in denitrification substratum, by measuring the content of nitrate, nitrite, total nitrogen in substratum, finally obtain a strain low-temperature aerobic denitrifying bacteria, this bacterium is delivered to China typical culture collection center on March 9th, 2015 and carries out preservation, Classification And Nomenclature: Pseudomonas brassicacearum subsp.brassicacearum BW0806, deposit number is: CCTCC NO:M 2015094, address: Wuhan, China Wuhan University.
Described low-temperature aerobic denitrifying bacteria is Gram-negative bacteria, and Adaptable growth temperature is 10 ~ 15 DEG C.Cultivate in LB solid medium, its colonial morphology is: smooth surface, yellow-white.Physiological and biochemical property is: aerobic, oxidase positive, catalase positive, has nitrate reduction ability.
Described LB solid medium consists of: Tryptones 10g, NaCl 10g, yeast extract 5g, agar powder 15g, is dissolved in 1000mL distilled water, regulates PH7.0.
Described denitrification substratum consists of: KNO30.5g; KH2PO41g; MgSO47H2O 0.1g; NH4Cl 0.2g; Sodium acetate 4g, trace element solution 2ml are dissolved in 1000mL distilled water, regulate PH7.2.Trace element solution forms: EDTA 50g; CaCl25.5g; ZnSO42.2g; MnCl24H2O 5.06g; FeSO47H2O 5g; (NH4) 6Mo7O24H2O 1.1g; CuSO45H2O 1.57g, CoCl26H2O 1.61g, is dissolved in 1000mL distilled water; PH7.2.
Low-temperature aerobic denitrifying bacteria provided by the invention when temperature is 15 DEG C, at initial NO3
-n cultivates in the denitrification substratum of 80mg/L, and after 8d, the degradation rate of nitrate is 100%, the degradation rate about 97% of total nitrogen.Under the culture temperature of 10 DEG C, in denitrification substratum, after 8d, the degradation rate of nitrate is 100%, the degradation rate about 60% of total nitrogen.In the growths of 30 DEG C and denitrification situation not as good as cold condition, belong to addicted to psychrophile.
Low-temperature aerobic denitrifying bacteria in the present invention adapts to low ratio of carbon to ammonium (C/N=2 ~ 10), particularly at C/N=5,15 DEG C, nitrate starting point concentration is under the culture condition of 80mg/L, in 3d, nitrate can be completely degraded, total nitrogen content degraded about 95%.
Low-temperature aerobic denitrifying bacteria in the present invention adapts to aerobic environment, 15 DEG C, nitrate starting point concentration is 80mg/L, dissolved oxygen amount >2mg/L, under the culture condition of C/N=5, in 4d, nitrate can be completely degraded, total nitrogen content degraded about 82%, comparatively anaerobic environment can grow and anti-nitration reaction better.
Low-temperature aerobic denitrifying bacteria in the present invention 15 DEG C, can the nitrate of load high density under the aerobic condition (dissolved oxygen amount >2mg/L) of C/N=5, nitrate starting point concentration is in the denitrification substratum of 300mg/L, and 72h nitrate removal rate is about 75%.Be that in 160mg/L, in concentration denitrification substratum, 72h can remove the nitrate of 100% at nitrate starting point concentration.Be that in the low concentration denitrification substratum of 30mg/L, 72h can remove nitrate and the total nitrogen content of 100% at nitrate starting point concentration.
Low-temperature aerobic denitrifying bacteria carries out the application in biological reinforced denitrogenation in the eutrophication water or nitrogenous effluent of low temperature, and low-temperature aerobic denitrifying bacteria BW0806 is cultured to increased logarithmic phase, with 10
4~ 10
6the throwing bacterium amount of cfu/mL drops in eutrophic water, nitrate, total nitrogen content and the COD that can effectively degrade in eutrophic water.
In above-described application, preferably, the environment in water body is manually adjusted to C/N=2 ~ 10.
Compared with prior art, the present invention has the following advantages:
(1) low temperature 15 DEG C, initial nitrate concentration is under the aerobic condition of 30mg/L, can degrade in low-temperature aerobic denitrifying bacteria Pseudomonas brassicacearum subsp.brassicacearum BW080672h 100% nitrate and total nitrogen content, reach the efficient removal of nitrate and total nitrogen under cold condition.
(2) the low-temperature aerobic denitrifying bacteria in the present invention belongs to addicted to psychrophile, adapts to the growing environment of 10 ~ 15 DEG C, for winter rivers and lakes, sewage effluent denitrogenation new thinking is provided, broken biological method low temperature administers the limitation of sewage.
(3) the low-temperature aerobic denitrifying bacteria in the present invention can under the culture condition of low ratio of carbon to ammonium amount reproduction, for waste water control reduces cost.
(4) the low-temperature aerobic denitrifying bacteria in the present invention adapts to aerobic condition, has to be applied to eutrophication water and in aerobic treatment tank, to combine nitrobacteria and carries out synchronous nitration-denitrifying potentiality.
(5) the low-temperature aerobic denitrifying bacteria in the present invention is applied in eutrophic water, can effectively denitrogenation and degraded COD under cryogenic.
Embodiment
Below in conjunction with embodiment, this explanation is further detailed, but use range of the present invention is not limited only to this.
Embodiment 1:
The acquisition of low-temperature aerobic denitrifying bacteria:
A. breathe out collected specimens river body from Qinghai cloth, be separated single bacterium colony and identify.
B. bacterial strain step A be separated to is cultivated in 15 DEG C of beef extract-peptone liquid nutrient mediums, obtains the psychrophile that can grow at 15 DEG C.
C. the psychrophile that step B filters out being inoculated in denitrification substratum, 15 DEG C of cultivations, by measuring the content of nitrate, nitrite, total nitrogen in substratum, judging to screen efficient low temperature denitrifying bacteria.
Finally obtain a strain low temperature denitrifying bacteria, this bacterium is delivered to China typical culture collection center on March 9th, 2015 and carries out preservation, Classification And Nomenclature: Pseudomonas brassicacearum subsp.brassicacearum BW0806, deposit number is: CCTCC NO:M 2015094, address: Wuhan, China Wuhan University.
Described low-temperature aerobic denitrifying bacteria is Gram-negative bacteria, and Adaptable growth temperature is 10 ~ 15 DEG C, and substratum is denitrification substratum.Its colonial morphology is: smooth surface, yellow-white.Physiological and biochemical property is: aerobic, oxidase positive, catalase positive, has nitrate reduction ability.
Denitrification substratum consists of: KNO
30.5g; KH
2pO
41g; MgSO47H2O 0.1g; NH
4cl 0.2g; Sodium acetate 4g, trace element solution 2ml are dissolved in 1000mL distilled water, regulate pH7.2.Trace element solution forms: EDTA 50g; CaCl
25.5g; ZnSO
42.2g; MnCl
24H
2o 5.06g; FeSO
47H
2o 5g; (NH
4) 6Mo
7o
24H
2o 1.1g; CuSO
45H
2o 1.57g, CoCl
26H
2o 1.61g is dissolved in 1000mL distilled water; PH7.2.
Embodiment 2:
The growth optimal temperature of Pseudomonas brassicacearum subsp.Brassicacearum BW0806:
This low-temperature aerobic denitrifying bacteria is cultured to increased logarithmic phase to be inoculated in denitrification substratum and to regulate initial OD
600about 0.05,120rpm seals shake-flask culture at different temperature, and every 24h measures nitrate, nitrite, total nitrogen content and OD600 value (table 1) in substratum.The above parameter value measured is (except OD
600) be the correlation in supernatant liquor after centrifugal thalline.
Table 1
Result shows this low-temperature aerobic denitrifying bacteria and adapts at 15 DEG C of growths and anti-nitration reaction, is 100% at the degradation rate of eight days nitrate, the degradation rate about 97% of total nitrogen.Under the culture temperature of 10 DEG C in denitrification substratum, be 100% at the degradation rate of the 8th day nitrate, the degradation rate about 60% of total nitrogen.In the growths of 30 DEG C and denitrification situation not as good as cold condition, belong to addicted to psychrophile.
Embodiment 3:
The growth of Pseudomonas brassicacearum subsp.brassicacearumBW0806 is suitable for carbon-nitrogen ratio:
Under 15 DEG C of conditions, this low-temperature aerobic denitrifying bacteria is cultured to increased logarithmic phase be inoculated in respectively different carbon-nitrogen ratio (carbon-nitrogen ratio by add different mass sodium acetate regulate) denitrification substratum in regulate initial OD 600 about 0.05,120rpm seals shake-flask culture, and every 24h measures nitrate, nitrite, total nitrogen content and OD600 value in substratum.The above parameter value measured is (except OD
600) be the correlation in supernatant liquor after centrifugal thalline.
Table 2
Detected result shows this low-temperature aerobic denitrifying bacteria and adapts to growth and anti-nitration reaction in low ratio of carbon to ammonium (2 ~ 10) substratum.When C/N=5, be 100% at the clearance of the 3rd day nitrate, the clearance of total nitrogen is 95%.
Embodiment 4:
The growth of Pseudomonas brassicacearum subsp.brassicacearum BW0806 is suitable for dissolved oxygen amount:
Under 15 DEG C of conditions, carbon-nitrogen ratio is under the culture condition of 5, cultivate ventilative in the denitrification substratum of different dissolved oxygen amount for this low-temperature aerobic denitrifying bacteria, regulate initial OD 600 about 0.05, every 24h measures nitrate, nitrite, total nitrogen content and OD600 value in substratum.The above parameter value measured is (except OD
600) be the correlation in supernatant liquor after centrifugal thalline.
Table 3
Detected result shows this bacterium and adapts to growth and anti-nitration reaction in the culture condition of aerobic (DO>2mg/L), wherein can be completely degraded at the 4th day nitrate, total nitrogen content degraded about 82%.Under anaerobic condition, the denitrification situation of this bacterium and growing state are compared with at aerobic culture condition allowance below nominal size.
Embodiment 5:
The growth of Pseudomonas brassicacearum subsp.brassicacearumBW0806 is suitable for nitrate concentration:
Under 15 DEG C of conditions, carbon-nitrogen ratio is 5, under the culture condition of aerobic (DO>2mg/L), this low-temperature aerobic denitrifying bacteria is inoculated in respectively low (30mg/L), in (160mg/L), high (300mg/L) concentration nitrate denitrification substratum in regulate initial OD 600 about 0.05,120rpm seals shake-flask culture, every 12h measures nitrate, nitrite, total nitrogen content and OD600 value in substratum, and the above parameter value measured is (except OD
600) be the correlation in supernatant liquor after centrifugal thalline.
Table 4
It is best that result shows this bacterium denitrification ability under the culture condition of 30mg/L protonitrate concentration, 72h can degrade 100% nitrate.Nitrate removal ability in centering concentration nitrate culture-medium (containing 160mg/L nitrate) does well, can the nitrate of degradable 160mg/L concentration.75% is about to the nitrate degradation rate of high concentration nitrate substratum (containing 300mg/L nitrate).In the denitrification substratum of basic, normal, high concentration nitrate, this low-temperature aerobic denitrifying bacteria all shows outstanding growing state.
Embodiment 6:
Gather eutrophication water sample, measure PH, temperature, nitric nitrogen, nitrite nitrogen, ammonia nitrogen, total nitrogen and COD in water sample.Add nitrate content in saltpetre to water sample and be about 10mg/L, object is its low temperature Denitrification Characteristics serious, adds sodium acetate and regulates C/N=5 (mass ratio) in water sample.Pseudomonas brassicacearum subsp.Brassicacearum BW0806 is cultured to increased logarithmic phase, with 10 in denitrification substratum
4~ 10
6the throwing bacterium amount of cfu/mL drops into that initial nitrate concentration is about 10mg/L, initial total nitrogen concentration is about in the eutrophic water of 18mg/L, and temperature about 10 DEG C, after pH value about 8.0,12d, ammonia-nitrogen content is 0, and the content of nitrate, total nitrogen and COD all reduce about 80%.
Claims (5)
1. a strain low-temperature aerobic denitrifying bacteria, is characterized in that: the Classification And Nomenclature of described low-temperature aerobic denitrifying bacteria is
pseudomonas brassicacearumsubsp.
brassicacearumbW0806, deposit number is: CCTCC NO:M 2015094.
2. the application of low-temperature aerobic denitrifying bacteria according to claim 1 in eutrophic water body denitrogenation.
3. the application of low-temperature aerobic denitrifying bacteria according to claim 1 in nitrogenous effluent denitrogenation.
4. the application according to Claims 2 or 3, the dosage of described low-temperature aerobic denitrifying bacteria is 10
4~ 10
6cfu/mL.
5. the application according to Claims 2 or 3, described water body or waste water are through being manually adjusted to: C/N=2 ~ 10.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109182199A (en) * | 2018-09-29 | 2019-01-11 | 中国科学院成都生物研究所 | One plant of rape pseudomonad with Plant growth promotion |
| CN112625942A (en) * | 2020-12-01 | 2021-04-09 | 华南理工大学 | Aerobic denitrifying bacterium and application thereof |
| US20220153617A1 (en) * | 2020-11-17 | 2022-05-19 | Zhejiang Gongshang University | Dynamic membrane reactor with function of nitrogen and phosphorus removal and operation method thereof |
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| CN102061276A (en) * | 2010-11-15 | 2011-05-18 | 北京大学 | Pseudomonas sp. strain for biological denitrification under low temperature and application thereof |
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| CN102061276A (en) * | 2010-11-15 | 2011-05-18 | 北京大学 | Pseudomonas sp. strain for biological denitrification under low temperature and application thereof |
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| Title |
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| 吐尔逊阿依•吾甫尔等: "一株耐盐好氧反硝化细菌的分离鉴定及生长特性研究", 《新疆农业科学》 * |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109182199A (en) * | 2018-09-29 | 2019-01-11 | 中国科学院成都生物研究所 | One plant of rape pseudomonad with Plant growth promotion |
| CN109182199B (en) * | 2018-09-29 | 2021-09-07 | 中国科学院成都生物研究所 | Pseudomonas brassicae with plant growth promoting effect |
| US20220153617A1 (en) * | 2020-11-17 | 2022-05-19 | Zhejiang Gongshang University | Dynamic membrane reactor with function of nitrogen and phosphorus removal and operation method thereof |
| US11795079B2 (en) * | 2020-11-17 | 2023-10-24 | Zhejiang Gonshang University | Dynamic membrane reactor with function of nitrogen and phosphorus removal and operation method thereof |
| CN112625942A (en) * | 2020-12-01 | 2021-04-09 | 华南理工大学 | Aerobic denitrifying bacterium and application thereof |
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| Publication number | Publication date |
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| CN104745507B (en) | 2018-01-09 |
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