CN103693752A - Holothurian culture water quality restoration agent for effectively removing water eutrophication and preparation method and application thereof - Google Patents

Holothurian culture water quality restoration agent for effectively removing water eutrophication and preparation method and application thereof Download PDF

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Publication number
CN103693752A
CN103693752A CN201310717900.3A CN201310717900A CN103693752A CN 103693752 A CN103693752 A CN 103693752A CN 201310717900 A CN201310717900 A CN 201310717900A CN 103693752 A CN103693752 A CN 103693752A
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restoration agent
culture
water quality
water
eutrophication
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Inventor
徐永平
尤建嵩
李淑英
李纪彬
周俊龙
徐牧
马欣
高玉平
刘士茹
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SAIMU BIOENGINEERING TECHNOLOGY Co Ltd DALIAN
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SAIMU BIOENGINEERING TECHNOLOGY Co Ltd DALIAN
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention discloses a holothurian culture water quality restoration agent for effectively removing water eutrophication and a preparation method and application of the holothurian culture water quality restoration agent, belonging to the technical field of aquaculture and marine microorganisms. Aiming at the physiological habit and culture characteristic of holothurian, a marine microorganism technology is applied, the restoration agent consists of bacillus flexus CGMCC No.5115, bacillus subtilis CGMCC No.5116 and bacillus cereus CGMCC No.5117 separated and screened from a holothurian culture environment, corn meal and soya bean meal serving as major culture raw materials are fermented at the temperature of 28-37 DEG C for 24-48 hours, and attapulgite, medical stone and rice hull serving as adsorption and suspension composite carriers are used for processing to obtain the holothurian culture water quality restoration agent for effectively removing water eutrophication. The product can adsorb harmful substances such as heavy ions including Hg<2+>, Pb<2+>, Cr<6+>, Cd<2+>, and Cu<2+> in water bodies, and the content of harmful substances such as ammonia nitrogen, nitrite, and hydrogen sulfide can be greatly reduced. Bottom odor and acidification of water bodies are avoided, pathogenic microorganisms are inhibited effectively, holothurian diseases are prevented, and the holothurian culture yield and quality are improved.

Description

Effectively remove the agent of holothruian cultures Water quality restoration and method for making and the purposes of body eutrophication
Technical field
The present invention, in aquaculture and Marine Microbial Biotechnology: A Review field, is specifically related to a kind of agent of holothruian cultures Water quality restoration and method for making and purposes of effective removal body eutrophication.
Background technology
Seawater is the Essential Environment condition that the precious marine products such as sea cucumber are depended on for existence, as the saying goes: " within three minutes, breeding fish, seven minutes foster water ".The quality of water quality directly has influence on the upgrowth situation of sea cucumber and raiser's economic benefit.Along with economy and the reach of science, people increase day by day to the demand of sea cucumber, natural, fish for can not meet the need of market in the situation that, holothruian cultures had been converted into the breeding way of present intensive and batch production already by traditional extensive management, Resource Dependence type breeding way, apostichopus japonicus culture has become one of mainstay industry of Chinese Fishery aquaculture.Yet; along with stichopus japonicus is intensive and large-scale cultivation develops rapidly; there are some serious problems, restricted the development of its industry, such as remaining bait and movement and animal dead volume deposit in water body; decompose and generate a lot of objectionable impuritiess; worsened water quality, pathogenic bacteria amount reproduction, causes sea cucumber to produce strong stress reaction; occurred shaking the head do not eat, mouthful swollen, health shrink stiff, arrange the phenomenons such as dirty, ulcer in body surface, last body wall big area is rotted and dead.In order to resist the sick epidemic disease of sea cucumber, long-term and a large amount of abuses of antibiotics and chemical synthetic drug, not only caused the resistance of sea cucumber to the susceptibility of pathogenic micro-organism and bacteria pathogeny microorganism etc., also can make microbiotic detect exceeds standard, and cause the pollution of whole ecotope, had a strong impact on the Sustainable development of saving reduction of discharging, food safety and having hindered nuisanceless apostichopus japonicus culture industry.
Attapulgite clay is under the jurisdiction of sepiolite group on mineralogy classification, and the magnesia silicate of layer chain for moisture, has unique layer chain-like structure feature, and its desirable chemical molecular formula is: Mg 5si 8o 20(OH) 2(OH 2) 24H 2o.Attapulgite is owing to having the crystalline structure that is needle-like, fibrous agrregate, the features such as its specific surface area is large, chemical stability good, high adsorption capacity, it shows good performance at aspects such as Adsorption of Heavy Metal Ions, organic pollutant, radioelement, aspect water treatment, has a good application prospect.Rice husk is one deck shell of paddy outside, by inner glume and larger coetonium composition.The long 5-10mm of rice husk, 2.5-5mm, thick 23-30 micron, it is golden yellow that its color and luster is generally, and after being pulverized, tap density can reach 384-400kg/m 3, as the auxiliary material of this product, can increase floatability, suspension and the sink-float controllability of product, be conducive to the decontamination effect improving of marine culture water 3 D stereo.
Medical stone is a kind of porousness aluminosilicate, Zhu Yao Shi ?parent cloud beschtauite, main chemical compositions is SiO 2and Al 2o 3, there is porousness, be spot shape or like spot structure, this special construction makes it have very strong physical adsorbability and ion exchangeable, the particulate form in can selective removal aquaculture water and pollution substance and ammonia nitrogen, nitrous acid nitrogen and the H of colloidal attitude 2s etc. cause the principal pollutant of body eutrophication, can also adsorption removal Hg 2+, Pb 2+, Cr 6+, Cd 2+, Cu 2+etc. objectionable impuritiess such as heavy metal ion, aspect water treatment, have a good application prospect.
Aquatic products probiotics, it is the active bacteria formulation that utilizes the beneficial microorganism in aquatic animal body or in aquaculture water to form through special processing process, there is the effects such as ammonia oxidation, nitrated, denitrification, phosphorus decomposing, sulphur removal and denitrogenation, effectively reduce the concentration of COD, ammonia nitrogen, nitrous acid nitrogen and sulfide in water, thereby effectively improve water quality.At present, be applied to the microecological water matter conditioning agent of aquaculture, be only limited to simple microbial inoculum or carrier microbial inoculum, in using, there is the inferior positions such as flora Survival Reproduction bad adaptability and quantity minimizing in the former, the latter be substantially major part be sink to pond at the bottom of, centering upper strata water body purification rate is low, cuts pool bottom environment dissolved oxygen low, is unfavorable for the breeding of microecological microbial agent.So, be badly in need of a kind of probiotics and can 3 D stereo is comprehensive can remove holothruian cultures COD in water body, ammonia nitrogen, nitrous acid nitrogen and sulfide, can remove Heavy Metals in Waters ion again, and suppress harmful microbe breeding, reach simultaneously the once Water quality restoration agent of purifying aquatic water completely.
Summary of the invention
The present situation such as in prior art, cultivation water is seriously polluted, disease takes place frequently in order to solve in the present invention, sea cucumber high mortality, sea cucumber heavy metal of body, chemical residual and antibiotic remains exceed standard, and the agent of holothruian cultures Water quality restoration and method for making and the purposes of inventing a kind of effective removal body eutrophication.This holothruian cultures Water quality restoration agent of effectively removing body eutrophication is mainly mixed and is formed by genus bacillus, separated also subtilis (Bacillus subtilis) YB-1CGMCCNo.5116, bacillus cereus (Bacilluscereus) YB-2CGMCC No.5117 and Bacillus flexus (Bacillus flexus) XH-1CGMCCNo.5115 of screening in holothruian cultures environment, take Semen Maydis powder and bean cake powder as main fermention medium, using attapulgite, medical stone and rice husk as absorption and suspending carrier, be processed into solid powder.The probiotics relating in the present invention, in 2011 08 month No. 05, deliver to China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation (Institute of Microorganism, Academia Sinica, postcode 100101), address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City.
Main aspect of the present invention is: a kind of preparation method of holothruian cultures Water quality restoration agent of effective removal body eutrophication, comprises following operation steps:
1. prepare probiotic bacterium stoste
Will be on LB agar slant culture-medium well-grown subtilis YB-1CGMCCNo.5116, bacillus cereus YB-2CGMCCNo.5117 and Bacillus flexus XH-1CGMCCNo.5115, add respectively 2116E substratum, pH7.0~7.5,12~16h is cultivated in 33~37 ℃ of concussions, with the volume ratio of 1:1:1, mix again, be prepared into probiotic bacterium stoste;
2. liquid state fermentation is cultivated
To add fermented liquid in closed fermentation reactor, and add nutritive substance by 0.1~1% volume ratio, then the probiotic bacterium stoste that 1. step is prepared, press the volume ratio inoculation of 3-5%, ferment, pressure is 0.1~0.25Mpa, 37 ℃ of fermentation 24h, make tunning;
3. spray drying treatment
The above-mentioned steps 2. tunning of gained is sprayed to absorption carrier, drying treatment;
Wherein, step is said seawater LB nutrient agar and 2116E substratum 1., is culture medium prescription in < < molecular cloning experiment guide > >;
Step 2. said nutritive substance by Semen Maydis powder: bean cake powder: seawater is by (1.5~3): (0.5-1.5): 100 weight ratio forms;
Step is consisting of of described absorption carrier 3.: the volume ratio of Attapulgite stone flour, medical stone powder and rice husk 2:2:1 is mixed.
Under optimal conditions: in above-mentioned preparation method, the step 1. cell concentration of middle bacteria suspension is all about 1 * 10 8cfu/ml.
Under optimal conditions: in above-mentioned preparation method, step is 3. in described absorption carrier, and Attapulgite stone flour and medical stone powder are 150~200 orders; End product absorption carrier and probiotic composition, the final concentration that carries bacterial content is about 10 8~10 10cfu/g.
Under optimal conditions: in above-mentioned preparation method, the weight ratio that adds absorption carrier in the product 3. making to step is 1:1~1000.
Under optimal conditions: in above-mentioned preparation method, the step method that 3. middle tunning is spray dried into absorption carrier is: fermentation culture is with vehicle weight than being 1:1~4, and be 12~24h time of drying, and product water content is not higher than 5%.
Other one side of the present invention is, utilizes above-mentioned method preparation effectively to remove the holothruian cultures Water quality restoration agent product of body eutrophication.
Other one side of the present invention is, the application of the holothruian cultures Water quality restoration agent that utilizes above-mentioned effective removal body eutrophication aspect holothruian cultures.
The holothruian cultures Water quality restoration agent environmental resistance of effective removal body eutrophication of the present invention is strong, floatability, suspension and sink-float controllability are good, decontamination effect improving to the aquaculture water 3 D stereo of sea cucumber is remarkable, especially at the nursery stage of sea cucumber and mature stage, regularly splashing, this effectively removes holothruian cultures Water quality restoration agent of body eutrophication, can remove fast heavy metal ion and degradation of ammonia nitrogen in aquaculture water, nitrite, the objectionable impuritiess such as hydrogen sulfide, avoid water acidification and the smelly end, effectively suppress pathogenic bacteria, alleviate the stress reaction of environment to sea cucumber, the effectively generation of prevention sea cucumber disease, improve the Quality and yield of holothruian cultures.
Embodiment
Below in conjunction with technical scheme, describe embodiments of the invention in detail.
Raw materials used by ordinary method preparation or obtained by commercial sources in embodiment.
LB agar slant culture-medium: it contains in forming every liter: pancreatin solution peptone 5g, yeast extract paste 1g, sea crystal 33g, high ferric phosphate 0.1g, agar 15g, 1000mL water, pH7.6.Peptone and yeast extract paste are purchased from Britain Oxoid company, and agar powder is purchased from Beijing extensive and profound in meaning star biotechnology limited liability company, and other raw materials are all purchased from Shanghai Sheng Gong biotechnology limited-liability company.
2216E liquid nutrient medium: it contains in forming every liter: pancreatin solution peptone 5g, yeast extract paste 1g, high ferric phosphate 0.1g, Chen Haishui 1000mL, pH7.6.Peptone and yeast extract paste are purchased from Britain Oxoid company, and other raw materials are all purchased from Shanghai Sheng Gong biotechnology limited-liability company.
Semen Maydis powder and bean cake powder, be 100-200 order, is purchased from Wandefu Ind Co., Ltd., Shandong.
Rice husk, is purchased from Dalian Sai Mu group.
Attapulgite, is purchased from Jingyuan County, Gansu Province Hao Di industry and trade limited liability company, 150-200 order.
Medical stone, is purchased from Inner Mongolia Autonomous Region Naimanqi Chinese Medical Stone Development Co., Ltd., 150-200 order.
The probiotics relating in the embodiment of the present invention comprises: in holothruian cultures environment, the genus bacillus of separation screening comprises: subtilis (Bacillus subtilis) YB-1CGMCCNo.5116, bacillus cereus (Bacillus cereus) YB-2CGMCCNo.5117 and Bacillus flexus (Bacillus flexus) XH-1CGMCCNo.5115.
Three strain bacterial strains are preserved in delivers to China Committee for Culture Collection of Microorganisms's common micro-organisms center (Institute of Microorganism, Academia Sinica, postcode 100101) for 2011 08 month No. 05, and address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City.
Below by example, the present invention is described in further detail.
Embodiment 1:
1. prepare probiotic bacterium stoste
Will be on LB agar slant culture-medium well-grown subtilis YB-1CGMCCNo.5116, bacillus cereus YB-2CGMCCNo.5117 and Bacillus flexus XH-1CGMCCNo.5115, add respectively 2116E substratum, pH7.0~7.5,12~16h is cultivated in 37 ℃ of concussions, with the volume ratio of 1:1:1, mix again, be prepared into probiotic bacterium stoste, cell concentration is about 1 * 10 8cfu/ml.
2. liquid state fermentation is cultivated
To add fermented liquid in closed fermentation reactor, and add nutritive substance by 0.1~1% volume ratio, then the probiotic bacterium stoste that 1. step is prepared, press the volume ratio inoculation of 3-5%, ferment, pressure is 0.1~0.25Mpa, 37 ℃ of fermentation 24h, make tunning;
Above-mentioned nutritive substance is by Semen Maydis powder: bean cake powder: seawater is by (1.5~3): (0.5-1.5): 100 weight ratio forms.
3. spray drying treatment
Mixture absorption carrier is 1:1:1 mixed preparing by 150-200 object Attapulgite stone flour, medical stone powder and rice husk by volume;
The tunning of above-mentioned steps 2 gained is spray dried in above-mentioned absorption carrier, and tunning is with vehicle weight than being 1:4, and be 24h time of drying, and product water content, not higher than 5%, makes the holothruian cultures Water quality restoration agent of effective removal body eutrophication.
Embodiment 2
1. prepare probiotic bacterium stoste
Will be on LB agar slant culture-medium well-grown subtilis YB-1CGMCCNo.5116, bacillus cereus YB-2CGMCCNo.5117 and Bacillus flexus XH-1CGMCCNo.5115, add respectively 2116E substratum, pH7.0~7.5,12~16h is cultivated in 37 ℃ of concussions, with the volume ratio of 1:1:1, mix again, be prepared into probiotic bacterium stoste, cell concentration is about 1 * 108cfu/ml.
2. liquid state fermentation is cultivated
To add fermented liquid in closed fermentation reactor, and add nutritive substance by 0.1~1% volume ratio, then the probiotic bacterium stoste that 1. step is prepared, press the volume ratio inoculation of 3-5%, ferment, pressure is 0.1~0.25Mpa, 37 ℃ of fermentation 24h, make tunning;
Above-mentioned nutritive substance is by Semen Maydis powder: bean cake powder: seawater is by (1.5~3): (0.5-1.5): 100 weight ratio forms.
3. spray drying treatment
Absorption carrier is 2:2:1 mixed preparing by 150-200 object Attapulgite stone flour, medical stone powder and rice husk by volume;
The tunning of above-mentioned steps 2 gained is spray dried in above-mentioned absorption carrier, and tunning is with vehicle weight than being 1:4, and be 24h time of drying, and product water content, not higher than 5%, makes the holothruian cultures Water quality restoration agent of effective removal body eutrophication.
Result of use
Choose the holothruian cultures circle that 3 place's conditions are identical, average depth 1.5-2m, seedling specification 30-50 head/500g, density is at 9-10 head/m 2.
No. 1 circle is control group (not using the holothruian cultures Water quality restoration agent of effectively removing body eutrophication), and 2, No. 3 circles are experimental group (using the holothruian cultures Water quality restoration agent of effectively removing body eutrophication); Experimental group is except the holothruian cultures Water quality restoration agent of the effective removal body eutrophication described in the embodiment 1,2 that regularly splashes, and the mode of feeding is the same with control group with management process.
Experimental period be in September, 2013 to October, about 45 days, experimental group was splashed 1 time every 3 days, the amount of splashing is 5g/m 3.
Experimental session, regularly detects water quality, observes the sea cucumber situation of ingesting, every 15 all over the world water touch spy, measure sea cucumber body weight.Salinity is 35-38 ‰, and water temperature is 16-23 ℃, and pH is 7.6-8.5, and dissolved oxygen is not less than 4mg/L.
1. use after the present embodiment 1,2 the water-quality guideline comparative result of the holothruian cultures circle of the 45th day
? PH value Ammonia nitrogen Nitrous acid nitrogen Hydrogen sulfide Transparency COD Mn
? ? (mg/L) (mg/L) (mg/L) (cm) (mg/L)
Control group 7.3 6.34 10.45 2.36 8.0 34.53
Embodiment 1 7.6 0.42 0.68 0.33 14.0 12.55
Embodiment 2 7.8 0.21 0.16 0.14 15.0 8.84
2. use the impact of latter 45 days of the present embodiment 1,2 on sea cucumber growth and immunity function
? Number/m2 Average weight gain amount (g) Respiratory burst vigor Phagocytic rate (%)
Control group 7.3 4.38 0.182±0.032 36.28±0.46
Embodiment 1 8.4 7.73 0.426±0.036 44.34±0.39
Embodiment 2 8.8 12.58 0.639±0.066 48.95±0.95
Result shows: the holothruian cultures Water quality restoration agent of effective removal body eutrophication of the present invention, water quality in holothruian cultures circle is had to obvious decontamination effect improving, can significantly reduce the content of the objectionable impuritiess such as ammonia nitrogen, nitrite, hydrogen sulfide, stablize water body pH, effectively suppress pathogenic bacteria, improve the immunizing power of sea cucumber, the generation of prevention sea cucumber disease, the Quality and yield of raising culturing sea cucumbers.

Claims (8)

1. effectively remove a preparation method for the holothruian cultures Water quality restoration agent of body eutrophication, comprise following operation steps:
1. prepare probiotic bacterium stoste
Will be on LB agar slant culture-medium well-grown subtilis YB-1CGMCCNo.5116, bacillus cereus YB-2CGMCCNo.5117 and Bacillus flexus XH-1CGMCCNo.5115, add respectively 2116E substratum, pH7.0~7.5,12~16h is cultivated in 33~37 ℃ of concussions, with the volume ratio of 1:1:1, mix again, be prepared into probiotic bacterium stoste;
2. liquid state fermentation is cultivated
To add fermented liquid in closed fermentation reactor, and add nutritive substance by 0.1~1% volume ratio, then the probiotic bacterium stoste that 1. step is prepared, press the volume ratio inoculation of 3-5%, ferment, pressure is 0.1~0.25Mpa, 37 ℃ of fermentation 24h, make tunning;
3. spray drying treatment
The above-mentioned steps 2. tunning of gained is sprayed to absorption and suspension complex carrier, drying treatment;
Wherein, step is said seawater LB nutrient agar and 2116E substratum 1., is culture medium prescription in < < molecular cloning experiment guide > >;
Step 2. said nutritive substance by Semen Maydis powder: bean cake powder: seawater is by (1.5~3): (0.5-1.5): 100 weight ratio forms;
Step is consisting of of described complex carrier 3.: Attapulgite stone flour, medical stone powder and rice husk are by the volume ratio mixing of 2:2:1.
2. preparation method according to claim 1, is characterized in that, step is described complex carrier 3., and Attapulgite stone flour and medical stone powder are 150~200 orders.
3. preparation method according to claim 2, is characterized in that, the step 1. cell concentration of middle bacteria suspension is 1 * 10 8cfu/ml.
4. according to the preparation method described in any one in claim 1,2 or 3, characterized by further comprising: in the product 3. making to step, add complex carrier, mix and make the final concentration of bacterial content for being about 10 8-10 10cfu/g.
5. preparation method according to claim 4, is characterized in that, the weight ratio that adds complex carrier in the product 3. making to step is 1:1~1000.
6. according to the preparation method described in any one in claim 1,2 or 5, it is characterized in that, the step method that 3. middle tunning is spray dried into complex carrier is: fermentation culture is with vehicle weight than being 1:1~4, and be 12~24h time of drying, and product water content is not higher than 5%.
7. the holothruian cultures Water quality restoration agent of effective removal body eutrophication that prepared by the method for claim 1.
8. the application of the holothruian cultures Water quality restoration agent of effective removal body eutrophication as claimed in claim 7 aspect holothruian cultures.
CN201310717900.3A 2013-12-20 2013-12-20 Holothurian culture water quality restoration agent for effectively removing water eutrophication and preparation method and application thereof Pending CN103693752A (en)

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Cited By (7)

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CN103931902A (en) * 2014-04-23 2014-07-23 大连金海湾养殖科技有限公司 Additive premix feed for regulating intestinal tract of holothurian, preparation method and application thereof
CN103931945A (en) * 2014-04-23 2014-07-23 大连金海湾养殖科技有限公司 Ecological intestinal regulator of sea cucumber and preparation method and application of ecological intestinal regulator
CN104789548A (en) * 2015-03-23 2015-07-22 大连理工大学 Carrier-immobilized bacterial inhibition type water purifier for marine aquaculture and preparation method thereof
CN106798195A (en) * 2016-12-28 2017-06-06 大连海宝渔业有限公司 Fermented laminaria drying means
CN106809958A (en) * 2017-01-19 2017-06-09 许星星 A kind of mycotic holothruian cultures Water quality restoration agent of anti-sea cucumber and its preparation method and application
CN109650556A (en) * 2019-01-23 2019-04-19 武汉丰甜生物科技有限公司 The composite bacillus microbial inoculum and its application of ammonia nitrogen and nitrite in degradation water body
CN110104797A (en) * 2019-05-30 2019-08-09 广东子辉生态环境科技有限公司 A kind of application method for the Tiny ecosystem flora except algae

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CN1961066A (en) * 2004-03-31 2007-05-09 丹尼斯科公司 Process
CN102616941A (en) * 2012-03-26 2012-08-01 大连理工大学 Sea cucumber microecological water quality conditioning agent and method for preparing same

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CN1961066A (en) * 2004-03-31 2007-05-09 丹尼斯科公司 Process
CN102616941A (en) * 2012-03-26 2012-08-01 大连理工大学 Sea cucumber microecological water quality conditioning agent and method for preparing same

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103931902A (en) * 2014-04-23 2014-07-23 大连金海湾养殖科技有限公司 Additive premix feed for regulating intestinal tract of holothurian, preparation method and application thereof
CN103931945A (en) * 2014-04-23 2014-07-23 大连金海湾养殖科技有限公司 Ecological intestinal regulator of sea cucumber and preparation method and application of ecological intestinal regulator
CN103931902B (en) * 2014-04-23 2016-03-09 大连金海湾养殖科技有限公司 Regulate additive premix of sea cucumber enteron aisle and its preparation method and application
CN104789548A (en) * 2015-03-23 2015-07-22 大连理工大学 Carrier-immobilized bacterial inhibition type water purifier for marine aquaculture and preparation method thereof
CN104789548B (en) * 2015-03-23 2017-07-21 大连理工大学 A kind of marine aquaculture carrier immobilized antibacterial type water quality cleansing agent and preparation method thereof
CN106798195A (en) * 2016-12-28 2017-06-06 大连海宝渔业有限公司 Fermented laminaria drying means
CN106798195B (en) * 2016-12-28 2019-08-13 大连海宝渔业有限公司 Fermented laminaria drying means
CN106809958A (en) * 2017-01-19 2017-06-09 许星星 A kind of mycotic holothruian cultures Water quality restoration agent of anti-sea cucumber and its preparation method and application
CN109650556A (en) * 2019-01-23 2019-04-19 武汉丰甜生物科技有限公司 The composite bacillus microbial inoculum and its application of ammonia nitrogen and nitrite in degradation water body
CN110104797A (en) * 2019-05-30 2019-08-09 广东子辉生态环境科技有限公司 A kind of application method for the Tiny ecosystem flora except algae

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