CN112079446A - Microbial regulator for improving watercourse water transparency, preparation method and application - Google Patents
Microbial regulator for improving watercourse water transparency, preparation method and application Download PDFInfo
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- CN112079446A CN112079446A CN202010825969.8A CN202010825969A CN112079446A CN 112079446 A CN112079446 A CN 112079446A CN 202010825969 A CN202010825969 A CN 202010825969A CN 112079446 A CN112079446 A CN 112079446A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/345—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for biological oxidation or reduction of sulfur compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
Abstract
The invention discloses a microbial regulator for improving the transparency of a river water body, a preparation method and application, and belongs to the field of biological agents. The method comprises the following steps: microbial agents and carriers; wherein the microbial agent comprises sulfur bacteria, nitrobacteria, nitrogen-fixing bacteria, bacillus subtilis, saccharomycetes, lactic acid bacteria and actinomycetes. The microbial community rapidly degrades various organic and inorganic nutrient substances, has obvious degradation effect, rapidly improves the transparency of the water body, promotes the survival rate of aquatic plants in the water body, provides a foundation for water ecological restoration, enables microbial inoculum microorganisms to well grow in various river sediment and water bodies, rapidly occupies favorable ecological niches, inhibits the growth and the reproduction of black and odorous microorganisms, reduces the odor release strength, and provides a foundation for ecological river regulation.
Description
Technical Field
The invention belongs to the field of biological agents, and particularly relates to a microbial regulator for improving the transparency of a river water body, a preparation method and application.
Background
With the development of modern society becoming faster and faster, the living standard of people is continuously improved, and a large amount of domestic sewage, agricultural irrigation water, domestic garbage, industrial wastewater and surface runoff with large pollution load directly flow into a water body, so that a large amount of organic pollutants are accumulated in rivers, and the rivers are black and odorous. The black and odorous water body is in an extreme water pollution state, and the country advocates a water ecological restoration method in two years, but in practical engineering application, how to improve the transparency of the water body is the key of the method for comprehensively remedying the black and odorous water body.
In water ecological restoration, the high-efficiency black and odorous bottom mud is an extremely complex heterogeneous body consisting of organic residues, bacterial thalli, inorganic particles, colloids and the like. The main characteristics of the sludge are high water content (up to more than 99 percent), high organic matter content, easy decomposition and stink generation, fine particles, small specific gravity, colloidal liquid state, thick matter between liquid and solid, and capability of being transported by a pump, but the solid-liquid separation by sedimentation is difficult.
At present, chemical agents such as polyaluminium chloride, polyacrylamide and the like are more applied, but the chemical agents are high in cost and large in dosage, generate secondary pollution and do not accord with an ecological treatment method. By reasonably configuring the types and the proportions of microorganisms, the transparency of the water body can be rapidly improved, and the loads of organic and inorganic nutrient salts in the water body, such as ammonia nitrogen, total nitrogen, organic phosphorus, permanganate indexes and the like, can be reduced.
Disclosure of Invention
The purpose of the invention is as follows: provides a microbial regulator for improving the transparency of a river water body, a preparation method and application thereof, so as to solve the problems related to the background technology.
The technical scheme is as follows: a microbial regulator for improving the transparency of a riverway water body comprises: microbial agents and carriers; wherein the microbial agent comprises sulfur bacteria, nitrobacteria, nitrogen-fixing bacteria, bacillus subtilis, saccharomycetes, lactic acid bacteria and actinomycetes.
As an excellent scheme, the microbial agent is prepared by the following formula:
sulfur bacterium 0.56X 108～1.52×108cfu/g；
Nitrifying bacteria 0.98X 108～2.11×108cfu/g；
Azotobacteria 0.34 × 108～0.88×108cfu/g；
Bacillus subtilis 1.28X 108～2.96×108cfu/g；
Yeast 1.03 × 108～2.19×108cfu/g；
Lactic acid bacteria 0.76X 108～1.58×108cfu/g；
Actinomycetes 1.02X 108～3.55×108cfu/g。
As an optimal speed scheme, the volume density of the carrier is 0.98-1.08 g/cm3The specific surface area is 180 to 200m2The porous suspended ceramsite is/g.
As an excellent speed scheme, the preparation process of the porous suspended ceramsite comprises the following steps:
step 1, weighing 60-75% of bentonite, 1-3% of hydroxyethyl cellulose sodium, 1-5% of polyoxyethylene ether, 10-15% of wheat bran and the balance of water according to mass percentage, uniformly mixing, pouring into a stirrer, and continuously stirring for 30-45 min to obtain uniformly dispersed slurry;
step 2, granulating by using granulation equipment, and then drying by using drying equipment;
3, placing the dried particles in a quartz tube, and calcining at a high temperature of 1000-1200 ℃ to remove organic matters in the particles;
step 4, polishing by a ball mill, removing the surface glaze layer of the surface particles, and exposing the holes on the surface;
step 5, putting the porous suspended ceramsite into a measuring cylinder filled with 250ml of deionized water, and screening the porous suspended ceramsite which sinks at the bottom after 8min or keeps suspended all the time, wherein the volume density of the porous suspended ceramsite is 0.98-1.05 g/cm3；
As an excellent scheme, the surface of the porous suspended ceramsite needs to be subjected to hydrophilization treatment.
As an excellent scheme, the surface treatment process of the porous suspended ceramsite comprises the following steps:
step 1, filling the porous suspended ceramsite into a measuring cylinder with 250ml of deionized water, and screening the porous suspended ceramsite which sinks or keeps suspended for 5min, wherein the volume density of the porous suspended ceramsite is 0.94-1.05 g/cm3；
Step 2, modification, namely adding the porous suspended ceramsite into the chitosan modified solution, mixing according to the bath ratio of 1 (20-30), heating to a low-boiling state, and stirring for 5-7 hours;
and 3, separating, filtering and screening out porous suspended ceramsite, cleaning with deionized water, and drying to obtain the modified bentonite ceramsite.
As an excellent speed scheme, the chitosan modified solution is prepared by dissolving 10-20 parts by mass of KH-550, 1-5 parts by mass of an emulsifier and 20-30 parts by mass of chitosan in a dilute acid solution and uniformly stirring.
The invention also provides a preparation method of the microbial regulator for improving the transparency of the river water body, which comprises the following steps:
step 1, respectively culturing sulfur bacteria, nitrobacteria, nitrogen-fixing bacteria, bacillus subtilis, saccharomycetes, lactic acid bacteria and actinomycetes in respective culture media to a saturated state;
step 2, adding the carrier into a conical flask containing the culture medium according to the proportion of 1-3 g of the carrier to every 100mL of the culture medium, placing the conical flask into a sterilization pot, sterilizing the conical flask for 20-30 min at the temperature of 100-120 ℃, taking out the conical flask, placing the conical flask on an aseptic operation table, and cooling the culture medium;
and 3, then carrying out the following steps of 1: 1: 1: 1: 1: 1: 1, inoculating the mixture into a culture medium, performing combined culture at the temperature of 20-35 ℃ until the composition proportion of each effective viable bacteria component is stable;
and 4, filtering and separating to obtain a carrier fixed with the strain, dehydrating the carrier by sequentially using ethanol solutions with increasing concentrations of 50-100%, and then carrying out vacuum freeze drying to obtain the microbial inoculum.
As an advantageous embodiment, the culture medium comprises the following components: 1000ml of river water body to be treated, 15-20 g of glucose, 2-3 g of monopotassium phosphate, 1.0-1.5 g of magnesium sulfate and 2-3 mg of vitamin B.
As an optimal speed scheme, the pH value of the culture medium is adjusted by acetic acid until the water body to be treated is the same.
The invention also provides an application of the microbial regulator in purifying water bodies and improving water body transparency, or an application in preparing a material for purifying water bodies and improving water body transparency.
Has the advantages that: the invention relates to a microbial regulator for improving the transparency of a river water body, a preparation method and application thereof. The ecological environment of the water area is controlled through simulation, a microbial ecological structure suitable for a target water body is naturally selected, the toxicity resistance and the tolerance of a microbial community are improved, and the impact on a biochemical system caused by the unstable sewage concentration is avoided. Meanwhile, by designing a proper carrier, the microbial community is solidified, the concentration and the purity of the microbes in the treated water area are improved, and the microbes are not easy to lose and pollute; the use time and the stability of the microbial preparation are improved, and the microbial addition cost can be reduced.
Drawings
Fig. 1 is a schematic structural view of the present invention.
Detailed Description
In the following description, numerous specific details are set forth in order to provide a more thorough understanding of the present invention. It will be apparent, however, to one skilled in the art, that the present invention may be practiced without one or more of these specific details. In other instances, well-known features have not been described in order to avoid obscuring the invention.
The single bacterial strain is not easy to survive in water purification and restoration, the purification effect is not as good as that of the composite bacteria, but how to reasonably configure the microbial species and the mixture ratio is always a problem in the industry, if the mixture ratio or the species are not proper, the antagonistic effect is formed between bacterial colonies, the survival competitive relationship exists between the bacterial colonies, the purification efficiency of the microorganisms can be reduced, and the survival rate of the bacterial colonies can be reduced.
On one hand, the invention provides a preparation method of a microbial regulator for improving the transparency of a river water body, which comprises the following steps:
step 1, respectively culturing sulfur bacteria, nitrobacteria, nitrogen-fixing bacteria, bacillus subtilis, saccharomycetes, lactic acid bacteria and actinomycetes in respective culture media to a saturated state; step 2, adding the carrier into a conical flask containing the culture medium according to the proportion of 1-3 g of the carrier to every 100mL of the culture medium, placing the conical flask into a sterilization pot, sterilizing the conical flask for 20-30 min at the temperature of 100-120 ℃, taking out the conical flask, placing the conical flask on an aseptic operation table, and cooling the culture medium; and 3, then carrying out the following steps of 1: 1: 1: 1: 1: 1: 1, inoculating the mixture into a culture medium, performing combined culture at the temperature of 20-35 ℃ until the composition proportion of each effective viable bacteria component is stable; and 4, filtering and separating to obtain a carrier fixed with the strain, dehydrating the carrier by sequentially using ethanol solutions with increasing concentrations of 50-100%, and then carrying out vacuum freeze drying to obtain the microbial inoculum.
In a further embodiment, the medium comprises the following components: 1000ml of river water body to be treated, 15-20 g of glucose, 2-3 g of monopotassium phosphate, 1.0-1.5 g of magnesium sulfate and 2-3 mg of vitamin B. And the pH value of the culture medium is adjusted to be the same as that of the water body to be treated by acetic acid. By the design, proper microorganism types and proportions can be screened out; the ecological environment of the water area is controlled through simulation, a microbial ecological structure suitable for a target water body is naturally selected, the toxicity resistance and the tolerance of a microbial community are improved, and the impact on a biochemical system caused by the unstable sewage concentration is avoided.
In another aspect, the present invention also provides a microbial regulator for improving the transparency of a river water body, comprising: microbial agents and carriers; wherein the microbial agent comprises sulfur bacteria, nitrobacteria, nitrogen-fixing bacteria, bacillus subtilis, saccharomycetes, lactic acid bacteria and actinomycetes.
In a further embodiment, the microbial agent is formulated as follows: sulfur bacterium 0.56X 108～1.52×108cfu/g; nitrifying bacteria 0.98X 108～2.11×108cfu/g；Azotobacteria 0.34 × 108～0.88×108cfu/g; bacillus subtilis 1.28X 108～2.96×108cfu/g; yeast 1.03 × 108～2.19×108cfu/g; lactic acid bacteria 0.76X 108～1.58×108cfu/g; actinomycetes 1.02X 108～3.55×108cfu/g。
In a further embodiment, the bulk density of the carrier is 0.98-1.08 g/cm3The specific surface area is 180 to 200m2The porous suspended ceramsite is/g. The preparation process of the porous suspended ceramsite comprises the following steps: step 1, weighing 60-75% of bentonite, 1-3% of hydroxyethyl cellulose sodium, 1-5% of polyoxyethylene ether, 10-15% of wheat bran and the balance of water according to mass percentage, uniformly mixing, pouring into a stirrer, and continuously stirring for 30-45 min to obtain uniformly dispersed slurry; step 2, granulating by using granulation equipment, and then drying by using drying equipment; 3, placing the dried particles in a quartz tube, and calcining at a high temperature of 1000-1200 ℃ to remove organic matters in the particles; step 4, polishing by a ball mill, removing the surface glaze layer of the surface particles, and exposing the holes on the surface; step 5, putting the porous suspended ceramsite into a measuring cylinder filled with 250ml of deionized water, and screening the porous suspended ceramsite which sinks at the bottom after 8min or keeps suspended all the time, wherein the volume density of the porous suspended ceramsite is 0.98-1.05 g/cm3；
In the case of the carrier immobilized microorganism process, among them, the performance of the immobilized carrier is a key factor for determining the service life of the microorganism, the treatment effect and the possibility of industrialization. The effective strains in the invention are anaerobic bacteria, and the floating of the carrier on the water surface or the sinking of the carrier into the water bottom are not suitable for the growth of the effective strains. In particular, the oxygen environment for water survival is not suitable for the growth of bacterial colonies; the colony microenvironment in the silt has great difference with the microenvironment in the water in the bottom, has a large amount of phages and native bacterial colony in the silt, and wherein, the phage and the native bacterial colony phagocytosis and the malignant competition to the microorganism can greatly reduce the survival rate of the bacterial colony. Therefore, the carrier with proper volume density and specific surface area is designed, and the sewage contains a great amount of organic matters, sewage tension and buoyancySlightly different from deionized water, when the volume density of the carrier is 0.98-1.08 g/cm3The specific surface area is 180 to 200m2The porous suspended ceramsite is characterized in that the porous suspended ceramsite can be kept in a suspended state for a long time under the action of sewage tension, buoyancy and turbulence, phagocytosis and malignant competition of bacteriophage and indigenous bacterial colonies in soil of a river bottom to microorganisms are shielded through a special microenvironment on the porous suspended ceramsite, the survival rate of the microorganisms is improved, the concentration and purity of the microorganisms in a treated water area are further improved, the microorganisms are not easy to lose and pollute, the using time of the preparation is long, the preparation is stable, and the adding cost of the microorganisms can be reduced.
In a further embodiment, the surface of the porous suspended ceramsite needs to be subjected to a hydrophilization treatment. The surface treatment process of the porous suspended ceramsite comprises the following steps: step 1, filling the porous suspended ceramsite into a measuring cylinder with 250ml of deionized water, and screening the porous suspended ceramsite which sinks or keeps suspended for 5min, wherein the volume density of the porous suspended ceramsite is 0.94-1.05 g/cm3(ii) a Step 2, modification, namely adding the porous suspended ceramsite into the chitosan modified solution, mixing according to the bath ratio of 1 (20-30), heating to a low-boiling state, and stirring for 5-7 hours; wherein, the chitosan modified solution is prepared by dissolving 10-20 parts of KH-550, 1-5 parts of emulsifier and 20-30 parts of chitosan in a diluted acid solution by mass parts and uniformly stirring; and 3, separating, filtering and screening out porous suspended ceramsite, cleaning with deionized water, and drying to obtain the modified bentonite ceramsite.
From the micro-immobilization mechanism of the microorganism, the charged microorganism cell and the charged carrier are formed through the physical actions of hydrogen bond, hydrophobic action, pi electron, affinity and the like or the electrostatic interaction between the two. In particular, an ionic bond or a covalent bond is formed between amino groups, carboxyl groups or other groups on the cell surface of the microorganism and hydroxyl reactive groups on the carrier. Therefore, the surface of the porous suspended ceramsite needs to be subjected to hydrophilization treatment, so that the microbial cells and the carriers are bonded, and the bonded porous suspended ceramsite is firmly solidified together.
The invention will now be further described with reference to the following examples, which are intended to be illustrative of the invention and are not to be construed as limiting the invention.
Example 1
A preparation method of a microbial regulator for improving the transparency of a river water body comprises the following steps:
step 1, culturing sulfur bacteria, nitrobacteria, nitrogen-fixing bacteria, bacillus subtilis, saccharomycetes, lactic acid bacteria and actinomycetes in respective culture media to a saturated state. Wherein the sulfur bacteria are hydrogen sulfide oxidizing bacteria, and the actinomycetes are thermophilic actinomycetes purchased from Guangzhou micro-Rewang biotechnology limited; nitrobacteria, azotobacter powder, bacillus subtilis powder, yeast powder and lactic acid bacteria powder are all purchased from Shanghai Ganmi environmental engineering Limited company, and the content of each bacterium is 50 hundred million/g; lactobacillus plantarum powder and Candida utilis powder are purchased from Anjian ring engineering counseling, Inc., Guangzhou city, and the content of each bacterium is 40 hundred million/g.
Step 2, according to the proportion of each 100mL of culture medium and 1.8g of carrier, adding the carrier into a conical flask containing the culture medium, placing the conical flask into a sterilization pot, sterilizing for 20min at 120 ℃, taking out the conical flask and placing the conical flask on an aseptic operation platform, and cooling the culture medium; wherein the culture medium comprises the following components: 1000ml of river water body to be treated, 18g of glucose, 2.2g of monopotassium phosphate, 1.3g of magnesium sulfate and 1.2mg of vitamin B; adjusting the pH value of the culture medium by acetic acid until the water bodies to be treated are the same; the volume density of the carrier is 0.98-1.08 g/cm3The specific surface area is 180 to 200m2The porous suspended ceramsite is characterized by comprising the following components in parts by weight, wherein the surface of the porous suspended ceramsite needs to be subjected to hydrophilization treatment.
And 3, then carrying out the following steps of 1: 1: 1: 1: 1: 1: 1, inoculating into a culture medium, performing combined culture, and culturing at 28 ℃ until the composition ratio of each effective viable bacteria component is stable; the composition of each colony is obtained by detection: sulfur bacterium 1.32X 108cfu/g; nitrifying bacteria 1.81X 108cfu/g; azotobacteria 0.67X 108cfu/g; bacillus subtilis 2.03X 108cfu/g; yeast 1.54 × 108cfu/g; lactic acid bacteria 1.12X 108cfu/g; actinomycetes 2.27X 108cfu/g。
And 4, filtering and separating to obtain a carrier fixed with the strain, dehydrating the carrier by sequentially using ethanol solutions with increasing concentrations of 50-100%, and then carrying out vacuum freeze drying to obtain the microbial inoculum.
The preparation process of the porous suspended ceramsite comprises the following steps: step 1, weighing 70% of bentonite, 2.5% of hydroxyethyl cellulose sodium, 3.0% of polyoxyethylene ether, 12% of wheat bran and 12.5% of water according to mass percentage, uniformly mixing, pouring into a stirrer, and continuously stirring for 45min to obtain uniformly dispersed slurry; step 2, granulating by using granulation equipment, and then drying by using drying equipment; step 3, placing the dried particles in a quartz tube, and calcining at high temperature of 1200 ℃ to remove organic matters in the particles; step 4, polishing by a ball mill, removing the surface glaze layer of the surface particles, and exposing the holes on the surface; and 5, filling the porous suspended ceramsite into a measuring cylinder with 250ml of deionized water, and screening the porous suspended ceramsite which sinks or keeps suspended for 5min, wherein the volume density of the porous suspended ceramsite is 0.94-1.05 g/cm3(ii) a Step 6, adding the porous suspended ceramsite into the chitosan modified solution, mixing according to a bath ratio of 1:30, heating to a low-boiling state, and stirring for 6 hours; wherein, the chitosan modified solution is prepared by dissolving 15 parts of KH-550, 2 parts of emulsifier and 25 parts of chitosan in a dilute acid solution in parts by mass and uniformly stirring; and 7, separating, filtering and screening out porous suspended ceramsite, cleaning with deionized water, and drying to obtain the modified bentonite ceramsite.
Example 2
A preparation method of a microbial regulator for improving the transparency of a river water body comprises the following steps:
step 1, culturing sulfur bacteria, nitrobacteria, nitrogen-fixing bacteria, bacillus subtilis, saccharomycetes, lactic acid bacteria and actinomycetes in respective culture media to a saturated state. Wherein the sulfur bacteria are hydrogen sulfide oxidizing bacteria, and the actinomycetes are thermophilic actinomycetes purchased from Guangzhou micro-Rewang biotechnology limited; nitrobacteria, azotobacter powder, bacillus subtilis powder, yeast powder and lactic acid bacteria powder are all purchased from Shanghai Ganmi environmental engineering Limited company, and the content of each bacterium is 50 hundred million/g; lactobacillus plantarum powder and Candida utilis powder are purchased from Anjian ring engineering counseling, Inc., Guangzhou city, and the content of each bacterium is 40 hundred million/g.
Step 2, according to the proportion of each 100mL of culture medium and 3g of carrier, adding the carrier into a conical flask containing the culture medium, placing the conical flask into a sterilization pot, sterilizing the conical flask at 120 ℃ for 30min, taking out the conical flask and placing the conical flask on an aseptic operation table, and cooling the culture medium; wherein the culture medium comprises the following components: 1000ml of river water body to be treated, 20g of glucose, 3g of monopotassium phosphate, 11.5g of magnesium sulfate and 3mg of vitamin B; adjusting the pH value of the culture medium by acetic acid until the water bodies to be treated are the same; the volume density of the carrier is 0.98-1.08 g/cm3The specific surface area is 180 to 200m2Per g of the porous suspended ceramsite, the surface of which needs to be hydrophilized, and the preparation method is the same as that of example 1.
And 3, then carrying out the following steps of 1: 1: 1: 1: 1: 1: 1, inoculating the mixture into a culture medium, performing combined culture at the temperature of 20-35 ℃ until the composition proportion of each effective viable bacteria component is stable; the composition of each colony is obtained by detection: sulfur bacterium 1.61X 108cfu/g; nitrifying bacteria 2.08X 108cfu/g; azotobacteria (0.98X 10)8cfu/g; bacillus subtilis 3.01 × 108cfu/g; yeast 1.27X 108cfu/g; lactic acid bacteria 1.23X 108cfu/g; actinomycetes 3.42X 108cfu/g。
And 4, filtering and separating to obtain a carrier fixed with the strain, dehydrating the carrier by sequentially using ethanol solutions with increasing concentrations of 50-100%, and then carrying out vacuum freeze drying to obtain the microbial inoculum.
Example 3
A preparation method of a microbial regulator for improving the transparency of a river water body comprises the following steps:
step 1, culturing sulfur bacteria, nitrobacteria, nitrogen-fixing bacteria, bacillus subtilis, saccharomycetes, lactic acid bacteria and actinomycetes in respective culture media to a saturated state. Wherein the sulfur bacteria are hydrogen sulfide oxidizing bacteria, and the actinomycetes are thermophilic actinomycetes purchased from Guangzhou micro-Rewang biotechnology limited; nitrobacteria, azotobacter powder, bacillus subtilis powder, yeast powder and lactic acid bacteria powder are all purchased from Shanghai Ganmi environmental engineering Limited company, and the content of each bacterium is 50 hundred million/g; lactobacillus plantarum powder and Candida utilis powder are purchased from Anjian ring engineering counseling, Inc., Guangzhou city, and the content of each bacterium is 40 hundred million/g.
Step 2, according to the proportion of each 100mL of culture medium and 3g of carrier, adding the carrier into a conical flask containing the culture medium, placing the conical flask into a sterilization pot, sterilizing for 30min at 100 ℃, taking out the conical flask and placing the conical flask on an aseptic operation table, and cooling the culture medium; wherein the culture medium comprises the following components: 1000ml of river water body to be treated, 15g of glucose, 2g of monopotassium phosphate, 1.0g of magnesium sulfate and 2mg of vitamin B; adjusting the pH value of the culture medium by acetic acid until the water bodies to be treated are the same; the volume density of the carrier is 0.98-1.08 g/cm3The specific surface area is 180 to 200m2Per g of the porous suspended ceramsite, the surface of which needs to be hydrophilized, and the preparation method is the same as that of example 1.
And 3, then carrying out the following steps of 1: 1: 1: 1: 1: 1: 1, inoculating into a culture medium, performing combined culture at 20 ℃ until the composition ratio of each effective viable bacteria component is stable; the composition of each colony is obtained by detection: sulfur bacterium 0.65X 108cfu/g; nitrifying bacteria 1.13X 108cfu/g; azotobacteria 0.41 × 108cfu/g; bacillus subtilis 1.24 × 108cfu/g; yeast 0.94 × 108cfu/g; lactic acid bacteria 0.84X 108cfu/g; actinomycetes 1.27X 108cfu/g。
And 4, filtering and separating to obtain a carrier fixed with the strain, dehydrating the carrier by sequentially using ethanol solutions with increasing concentrations of 50-100%, and then carrying out vacuum freeze drying to obtain the microbial inoculum.
Example 4
Based on the embodiment 1, the volume density of the carrier is 0.98-1.08 g/cm3The specific surface area is 180 to 200m2Per g of porous suspended ceramsite. The preparation method comprises the following steps: step 1, weighing 70% of bentonite, 2.5% of hydroxyethyl cellulose sodium, 3.0% of polyoxyethylene ether, 12% of wheat bran and 12.5% of water according to mass percentage, uniformly mixing, pouring into a stirrer, and continuously stirring for 45min to obtain uniformly dispersed slurry; step 2, granulating by using granulation equipment, and then drying by using drying equipment; step 3, placing the dried particles in a quartz tube, and calcining at high temperature of 1200 ℃ to remove organic matters in the particles; step 4, polishing by a ball mill, removing the surface glaze layer of the surface particles, and exposing the holes on the surface; and 5, filling the porous suspended ceramsite into a measuring cylinder with 250ml of deionized water, and screening the porous suspended ceramsite which sinks or keeps suspended for all the time after 8min, wherein the volume density of the porous suspended ceramsite is 0.98-1.05 g/cm3。
The remaining steps, species and materials were the same as in example 1.
Example 5
Based on the embodiment 1, the volume density of the carrier is 0.92-0.99 g/cm3The specific surface area is 180 to 200m2The porous suspended ceramsite is/g.
The remaining steps, species and materials were the same as in example 1.
Example 6
Based on the embodiment 1, the volume density of the carrier is 1.01-1.12 g/cm3The specific surface area is 180 to 200m2The porous suspended ceramsite is/g.
The remaining steps, species and materials were the same as in example 1.
Example 7
On the basis of example 1, the medium comprises the following components: 1000ml of deionized water, 25g of tryptone, 5g of yeast extract, 20g of beef peptone, 18g of glucose, 2.2g of monopotassium phosphate, 1.3g of magnesium sulfate and 1.2mg of vitamin B.
The remaining steps, species and materials were the same as in example 1.
Example of detection
And (3) laboratory detection: and (3) taking 2L of river water to be treated, and then respectively adding 2.5g of the microbial inoculum obtained in the embodiments 1 to 7, and detecting the mud land restoration effect. The specific assay data after 30 days are shown in the table below.
As can be seen from the above table, the microbial inoculum obtained in the embodiments 1 to 3 has excellent biodegradability, and can achieve the effect of stabilizing and restoring the soil ecological environment of the water body.
Engineering detection: the microbial inoculum obtained in the preferred embodiment 1 is applied to engineering, wherein specific experimental data are shown in the attached figure 1, and the figure 1 is a change curve of water quality indexes along with time. From the curve, after the sewage is treated by the microorganisms for 45 days, the COD (chemical oxygen demand) reduction rate reaches 59.3 percent, and NH (ammonia) is reduced3The reduction rates of-N, TN and TP are all more than 85%, and COD and NH in the sewage3The data content of-N, TN, TP and the like can reach the basic discharge standard of the state for V-type water.
In a word, the microbial community rapidly degrades various organic and inorganic nutrient substances, has obvious degradation effect, rapidly improves the transparency of the water body, promotes the survival rate of aquatic plants in the water body, provides a foundation for water ecological restoration, enables microbial inoculum microorganisms to grow well in various river sediment and water bodies, rapidly occupies favorable ecological niches, inhibits the growth and the reproduction of black and odorous microorganisms, reduces the odor release strength, and provides a foundation for ecological river regulation. The ecological environment of the water area is controlled through simulation, a microbial ecological structure suitable for a target water body is naturally selected, the toxicity resistance and the tolerance of a microbial community are improved, and the impact on a biochemical system caused by the unstable sewage concentration is avoided. Meanwhile, by designing a proper carrier, the microbial community is solidified, the concentration and the purity of the microbes in the treated water area are improved, and the microbes are not easy to lose and pollute; the use time and the stability of the microbial preparation are improved, and the microbial addition cost can be reduced.
It should be noted that the various features described in the above embodiments may be combined in any suitable manner without departing from the scope of the invention. The invention is not described in detail in order to avoid unnecessary repetition.
Claims (10)
1. A microbial regulator for improving the transparency of a river water body is characterized by comprising: microbial agents and carriers; wherein the microbial agent comprises sulfur bacteria, nitrobacteria, nitrogen-fixing bacteria, bacillus subtilis, saccharomycetes, lactic acid bacteria and actinomycetes.
2. The microbial regulator for improving the transparency of a riverway water body according to claim 1, which is prepared from the following formula:
sulfur bacterium 0.56X 108～1.52×108cfu/g；
Nitrifying bacteria 0.98X 108～2.11×108cfu/g；
Azotobacteria 0.34 × 108～0.88×108cfu/g；
Bacillus subtilis 1.28X 108～2.96×108cfu/g；
Yeast 1.03 × 108～2.19×108cfu/g；
Lactic acid bacteria 0.76X 108～1.58×108cfu/g；
Actinomycetes 1.02X 108～3.55×108cfu/g。
3. The microbial regulator for improving the transparency of a river water body according to claim 1, wherein the volume density of the carrier is 0.98-1.08 g/cm3The specific surface area is 180 to 200m2The porous suspended ceramsite is/g.
4. The microbial regulator for improving the transparency of a riverway water body according to claim 3, wherein the preparation process of the porous suspended ceramsite comprises the following steps:
step 1, weighing 60-75% of bentonite, 1-3% of hydroxyethyl cellulose sodium, 1-5% of polyoxyethylene ether, 10-15% of wheat bran and the balance of water according to mass percentage, uniformly mixing, pouring into a stirrer, and continuously stirring for 30-45 min to obtain uniformly dispersed slurry;
step 2, granulating by using granulation equipment, and then drying by using drying equipment;
3, placing the dried particles in a quartz tube, and calcining at a high temperature of 1000-1200 ℃ to remove organic matters in the particles;
step 4, polishing by a ball mill, removing the surface glaze layer of the surface particles, and exposing the holes on the surface;
step 5, putting the porous suspended ceramsite into a measuring cylinder filled with 250ml of deionized water, and screening the porous suspended ceramsite which sinks at the bottom after 8min or keeps suspended all the time, wherein the volume density of the porous suspended ceramsite is 0.98-1.05 g/cm3。
5. The microbial regulator for improving the transparency of a river water body according to claim 3, wherein the surface of the porous suspended ceramsite needs to be subjected to hydrophilization treatment.
6. The microbial regulator for improving the transparency of the river water body according to claim 5, wherein the surface treatment process of the porous suspended ceramsite comprises the following steps:
step 1, filling the porous suspended ceramsite into a measuring cylinder with 250ml of deionized water, and screening the porous suspended ceramsite which sinks or keeps suspended for 5min, wherein the volume density of the porous suspended ceramsite is 0.94-1.05 g/cm3；
Step 2, modification, namely adding the porous suspended ceramsite into the chitosan modified solution, mixing according to the bath ratio of 1 (20-30), heating to a low-boiling state, and stirring for 5-7 hours;
and 3, separating, filtering and screening out porous suspended ceramsite, cleaning with deionized water, and drying to obtain the modified bentonite ceramsite.
7. The microbial regulator for improving the transparency of a river water body according to claim 6, wherein the chitosan modified solution is prepared by dissolving 10-20 parts by mass of KH-550, 1-5 parts by mass of an emulsifier and 20-30 parts by mass of chitosan in a dilute acid solution and uniformly stirring.
8. The preparation method of the microbial regulator for improving the transparency of the river water body according to any one of claims 1 to 7, is characterized by comprising the following steps:
step 1, respectively culturing sulfur bacteria, nitrobacteria, nitrogen-fixing bacteria, bacillus subtilis, saccharomycetes, lactic acid bacteria and actinomycetes in respective culture media to a saturated state;
step 2, adding the carrier into a conical flask containing the culture medium according to the proportion of 1-3 g of the carrier to every 100mL of the culture medium, placing the conical flask into a sterilization pot, sterilizing the conical flask for 20-30 min at the temperature of 100-120 ℃, taking out the conical flask, placing the conical flask on an aseptic operation table, and cooling the culture medium;
and 3, then carrying out the following steps of 1: 1: 1: 1: 1: 1: 1, inoculating the mixture into a culture medium, performing combined culture at the temperature of 20-35 ℃ until the composition proportion of each effective viable bacteria component is stable;
and 4, filtering and separating to obtain a carrier fixed with the strain, dehydrating the carrier by sequentially using ethanol solutions with increasing concentrations of 50-100%, and then carrying out vacuum freeze drying to obtain the microbial inoculum.
9. The preparation method of the microbial regulator for improving the transparency of the river water body according to claim 8, wherein the culture medium comprises the following components: 1000ml of river water body to be treated, 15-20 g of glucose, 2-3 g of monopotassium phosphate, 1.0-1.5 g of magnesium sulfate and 2-3 mg of vitamin B;
and the pH value of the culture medium is adjusted to be the same as that of the water body to be treated by acetic acid.
10. The use of the microorganism regulator according to any one of claims 1 to 7 in purifying water bodies and improving the transparency of water bodies, or in preparing materials for purifying water bodies and improving the transparency of water bodies.
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