CN114561321A - Microbial preparation for treating black and odorous water body and preparation method thereof - Google Patents
Microbial preparation for treating black and odorous water body and preparation method thereof Download PDFInfo
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- 241000122229 Acinetobacter johnsonii Species 0.000 claims description 2
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- 239000010802 sludge Substances 0.000 abstract description 8
- 239000003344 environmental pollutant Substances 0.000 abstract description 6
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Genetics & Genomics (AREA)
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- General Health & Medical Sciences (AREA)
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- Environmental & Geological Engineering (AREA)
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Abstract
The invention relates to the technical field of sewage treatment, in particular to a microbial preparation for treating black and odorous water and a preparation method thereof; putting the mixed bacteria into a culture dish to culture strains for fermentation to obtain seed liquid; mixing the seed liquid with the carrier to prepare powder to obtain mixed bacterial powder; mixing and granulating the mixed bacteria powder, the adhesive, the disintegrating agent and the disintegration aid to obtain semi-finished product granules; the microbial particles prepared by the method can perform biochemical actions such as denitrification, ammonia oxidation, phosphorus accumulation, sulfur oxidation and the like, effectively remove pollutant substances such as organic matters, ammonia nitrogen, hydrogen sulfide, methyl sulfide, total phosphorus and the like and odor of the bottom sludge, and simultaneously avoid the problem that the bottom sludge is polluted by the microbial particles which are diffused into an upper water body after being put in the water and flow along with water flow and are difficult to act on the black and odorous water body.
Description
Technical Field
The invention relates to the technical field of sewage treatment, in particular to a microbial preparation for treating black and odorous water and a preparation method thereof.
Background
With the development of society and economy, in the process of industrialization and urbanization, a large amount of domestic sewage and industrial wastewater is discharged into flowing or static water bodies such as lakes and rivers in cities, so that the water body is seriously polluted to form black and odorous water body. Because of factors influences such as atmospheric sedimentation, surface runoff and rainwater sewage which are not completely shunted, pollutants can be continuously collected into water bodies of rivers and lakes, and in the pollution invasion and water self-purification processes, when excessive pollutants exceed the self-purification capacity of natural water bodies, the pollutants can be collected into bottom mud of the water bodies, so that the adsorption effect of the bottom mud of the water bodies is formed, and the polluted bottom mud is generated.
The black and odorous water polluted bottom sediment continuously releases polluted organic matters, nutritive salts and volatile malodorous gases to the upper water layer in the water body through the release effect of the bottom sediment, thereby aggravating the water body pollution. Therefore, the bottom sludge needs to be repaired in situ, the bottom sludge treatment microbial agent which can be sunk into the water bottom and slowly released is adopted, the capability of decomposing the pollutants of the bottom sludge by the composite microorganisms at the junction of the bottom sludge and the overlying water is enhanced, the pollutants of the black and odorous bottom sludge are prevented from being released to the upper water body, the organic bottom sludge is reduced at the same time, and the self-cleaning capability of the water body is improved. Because the overall dredging cost of the water body is too high, the construction quantity is large, and the water body is difficult to frequently adopt, the treatment means of the surface water body generally acts on the bottom mud of the black and odorous water body. The aeration and oxygenation of the water body are difficult to directly act on bottom sediment, the microbial inoculum for water body treatment in the current market is mostly liquid preparation or powder, the loss amount of functional microorganisms is large due to the flowing of the water body and the suspension characteristic of the microorganisms, and the rest microbial inoculum is difficult to act on the black and odorous water body polluted bottom sediment.
Therefore, a microbial preparation for treating black and odorous water and a preparation method thereof are provided.
Disclosure of Invention
The invention aims to provide a microbial preparation for black and odorous water treatment and a preparation method thereof, and aims to solve the problem that the existing microbial preparation for treatment is difficult to act on black and odorous water to pollute bottom mud due to the nature of the microbial preparation.
In order to achieve the above objects, in a first aspect, the present invention provides a method for preparing a microbial preparation for black and odorous water treatment, comprising the steps of:
putting the mixed bacteria into a culture dish to culture strains for fermentation to obtain seed liquid;
mixing the seed liquid with a carrier to prepare powder to obtain mixed bacterial powder;
putting the mixed bacteria powder, the adhesive, the disintegrating agent and the disintegration aid into a mixing granulation mode to obtain semi-finished product granules;
and (3) pretreating the semi-finished product particles to obtain the microbial particles.
The seed solution and the carrier are mixed to prepare powder, and the specific mode for obtaining the mixed bacterial powder is as follows:
putting the seed solution and the carrier into a stirring tank, and stirring and mixing to obtain a stirred mixture;
and putting the stirred mixture into a grinding machine for grinding into powder to obtain the mixed bacterial powder.
The specific mode of mixing and granulating the mixed bacterial powder, the adhesive, the disintegrating agent and the disintegration aid to obtain the semi-finished product particles is as follows:
putting the mixed bacterial powder, the adhesive, the disintegrating agent and the disintegration aid into a stirring tank for mixing to obtain a secondary mixture;
and (4) putting the secondary mixture into a granulator for granulation to obtain the semi-finished product granules.
The specific mode for pretreating the semi-finished product particles to obtain the microbial particles is as follows:
putting the semi-finished product particles into a dryer for drying treatment to obtain dried particles;
screening the dried particles through a filter to obtain the microbial particles.
Wherein the specific manner of screening the dried particles through a filter to produce the microbial particles is as follows:
removing larger particles through the filter body, and recovering the large particles to repeat the semi-finished particle step;
and recovering and storing the particles with smaller volume screened by the filter to prepare the microbial particles.
In a second aspect, the invention also provides a microbial preparation for treating the black and odorous water body according to the mass ratio, which comprises the following components; 20-30% of mixed bacteria, 47-69% of carrier, 6-12% of adhesive, 4-8% of disintegrating agent and 1-3% of disintegration aid; a preparation method of a microbial preparation for treating black and odorous water.
The carrier consists of diatomite, wheat bran powder, light calcium carbonate and glycerol, the adhesive is one or more of bentonite, sodium hydroxymethyl cellulose and xanthan gum, the disintegrating agent is one or two of lignocellulose, sodium hydroxymethyl starch and alginic acid, and the disintegration aid is one or two of sodium ascorbate and sodium citrate.
The invention relates to a microbial preparation for treating black and odorous water and a preparation method thereof, wherein mixed bacteria are put into a culture dish to culture bacterial strains for fermentation, so as to obtain seed liquid; mixing the seed liquid with the carrier to prepare powder to obtain mixed bacterial powder; mixing and granulating the mixed bacteria powder, the adhesive, the disintegrating agent and the disintegration aid to obtain semi-finished product granules; the preparation method has the advantages that raw materials are economical and easy to produce, the preparation can be slowly disintegrated on the black and odorous water body sediment, the microbial granules are prevented from being diffused into an upper water body and flowing along with water flow after being put in, and the problem that the existing treatment microbial agent is difficult to act on the black and odorous water body to pollute the sediment due to the self property is solved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a flow chart of a method for preparing a microbial preparation for black and odorous water treatment provided by the invention.
FIG. 2 is a flow chart of a specific method for mixing the seed solution with the carrier to prepare powder to obtain mixed bacterial powder.
Fig. 3 is a flow chart of a specific mode of mixing and granulating the mixed bacterial powder, the adhesive, the disintegrating agent and the disintegration aid to obtain a semi-finished product granule.
FIG. 4 is a flow chart of a specific manner of pre-treating the semi-finished pellets to obtain the microorganism pellets.
FIG. 5 is a flow chart showing a specific manner of screening the dried granules through a filter to produce the microbial granules.
Fig. 6 is a schematic diagram of a filter construction.
In the figure: 1-filter screen, 2-bracket, 3-vibration component, 4-collecting tank, 5-motor, 6-eccentric wheel, 7-tank body and 8-pulley.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.
Referring to fig. 1 to 5, in a first aspect, the present invention provides a method for preparing a microbial preparation for black and odorous water treatment, including the following steps:
s1, putting the mixed bacteria into a culture dish to culture strains for fermentation to obtain seed liquid;
s2, mixing the seed liquid with a carrier to prepare powder to obtain mixed bacterial powder;
the specific mode is as follows:
s21, putting the seed liquid and the carrier into a stirring tank, and stirring and mixing to obtain a stirred mixture;
s22, putting the stirred mixture into a grinding machine to grind into powder, and obtaining the mixed bacterial powder.
S3, mixing and granulating the mixed bacterial powder, the adhesive, the disintegrating agent and the disintegration aid to obtain semi-finished product particles;
the specific mode is as follows:
s31, putting the mixed bacterial powder, the adhesive, the disintegrating agent and the disintegration aid into a stirring tank for mixing to obtain a secondary mixture;
s32, putting the secondary mixture into a granulator for granulation to obtain the semi-finished product granules.
S4, pretreating the semi-finished product particles to obtain the microbial particles.
The specific mode is as follows:
s41, putting the semi-finished product particles into a dryer for drying treatment to obtain dried particles;
s42, screening the dried particles through a filter to obtain the microbial particles;
s421, removing particles with larger volume through the filter body of the filter, and recovering large particles to repeat the semi-finished product particle step;
s422, recovering and storing the particles with smaller volume screened by the filter to prepare the microorganism particles.
In a second aspect, the microbial preparation for black and odorous water treatment comprises, by mass; 20-30% of mixed bacteria, 47-69% of carrier, 6-12% of adhesive, 4-8% of disintegrating agent and 1-3% of disintegration aid; a preparation method of a microbial preparation for treating black and odorous water.
The mixed bacteria are prepared by mixing pseudomonas stutzeri, rhodopseudomonas palustris, lactobacillus plantarum, acinetobacter johnsonii, alcaligenes and bacillus megaterium in equal proportion, the carrier consists of diatomite, wheat bran powder, light calcium carbonate and glycerol, the adhesive is one or more of bentonite, sodium hydroxymethyl cellulose and xanthan gum, the disintegrating agent is one or two of lignocellulose, sodium hydroxymethyl starch and alginic acid, and the disintegration assistant is one or two of sodium ascorbate and sodium citrate.
Specifically, the total nitrogen in the bottom sediment can be well removed by the denitrification of pseudomonas stutzeri and the autotrophy of rhodopseudomonas palustris by using ammonia nitrogen under the anaerobic or anoxic condition; the lactobacillus plantarum plays an important role in the decomposition process of organic matters in the bottom mud; in anoxic and micro-aerobic acinetobacter junii, alcaligenes and bacillus megaterium, the degradation of refractory organic matters, the solidification of total phosphorus, the reduction of nitrite and the like can be carried out, and several functional strains in the preparation are cooperated with each other.
Referring to fig. 6, the filter includes a filter screen 1, two brackets 2, two vibration assemblies 3 and a collecting tank 4, the filter screen 1 is fixedly connected to the two brackets 2 and is located on the two brackets 2, the two vibration assemblies 3 are respectively disposed on the bracket 2 near one side of the filter screen 1, and the collecting tank 4 is disposed at the bottom of the filter screen 1.
In this embodiment, the assembly provides the installation conditions for the filter screen 1 and the vibration assembly 3, the vibration assembly 3 makes the filter screen 1 vibrate and filter the semi-finished product particles, and the collection tank 4 is used for recovering and storing the semi-finished product particles passing through the filter screen 1.
Vibrations subassembly 3 includes motor 5 and eccentric wheel 6, motor 5 with 2 fixed connection of support, and be located and be close to filter screen 1 one side, eccentric wheel 6 with 5 output end fixed connection of motor, and be located and be close to filter screen 1 one side.
In this embodiment, 5 circular telegrams of motor output end drives eccentric wheel 6 rotates, through eccentric wheel 6 ceaselessly with filter screen 1 collides, thereby makes filter screen 1 carries out the vibrations of high frequency and filters semi-manufactured goods granule.
The collecting tank 4 comprises a tank body 7 and a pulley 8, wherein the pulley 8 is rotatably connected with the tank body 7 and is positioned at the bottom of the tank body 7.
In this embodiment, the tank 7 is used for collecting the semi-finished product particles passing through the filter screen 1, and the pulley 8 is arranged to facilitate the moving and recycling of the tank 7.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (7)
1. The preparation method of the microbial preparation for treating the black and odorous water body is characterized by comprising the following steps of:
putting the mixed bacteria into a culture dish to culture strains for fermentation to obtain seed liquid;
mixing the seed liquid with a carrier to prepare powder to obtain mixed bacterial powder;
mixing the mixed bacteria powder, an adhesive, a disintegrating agent and a disintegration aid to obtain semi-finished product granules;
and (3) pretreating the semi-finished product particles to obtain the microbial particles.
2. The method for preparing a microbial preparation for black and odorous water treatment according to claim 1,
the seed solution and the carrier are mixed to prepare powder, and the concrete mode of obtaining the mixed bacterial powder is as follows:
putting the seed liquid and the carrier into a stirring tank, and stirring and mixing to obtain a stirred mixture;
and putting the stirred mixture into a grinding machine for grinding into powder to obtain the mixed bacterial powder.
3. The method for preparing a microbial preparation for black and odorous water treatment according to claim 1,
the specific mode of mixing and granulating the mixed bacterial powder, the adhesive, the disintegrating agent and the disintegration aid to obtain the semi-finished product particles is as follows:
putting the mixed bacterial powder, the adhesive, the disintegrating agent and the disintegration aid into a stirring tank for mixing to obtain a secondary mixture;
and (4) putting the secondary mixture into a granulator for granulation to obtain the semi-finished product granules.
4. The method for preparing a microbial preparation for black and odorous water treatment according to claim 1,
the specific mode for pretreating the semi-finished product particles to obtain the microbial particles is as follows:
putting the semi-finished product particles into a dryer for drying treatment to obtain dried particles;
screening the dried particles through a filter to obtain the microbial particles.
5. The method for preparing a microbial preparation for black and odorous water treatment according to claim 1,
the specific mode for screening the dried particles through the filter to prepare the microbial particles is as follows:
removing larger particles through the filter body, and recovering the large particles to repeat the semi-finished particle step;
and recovering and storing the particles with smaller volume screened by the filter to prepare the microorganism particles.
6. The microbial preparation for black and odorous water body treatment, which is applied to the preparation method of the microbial preparation for black and odorous water body treatment according to any one of claims 1 to 5,
the composite microbial carrier comprises, by mass, 20-30% of mixed bacteria, 47-69% of a carrier, 6-12% of an adhesive, 4-8% of a disintegrating agent and 1-3% of a disintegration aid; a preparation method of a microbial preparation for treating black and odorous water.
7. The microbial preparation for black and odorous water body treatment according to claim 1,
the mixed bacteria are prepared by mixing pseudomonas stutzeri, rhodopseudomonas palustris, lactobacillus plantarum, acinetobacter johnsonii, alcaligenes and bacillus megaterium in equal proportion, the carrier consists of diatomite, wheat bran powder, light calcium carbonate and glycerol, the adhesive is one or more of bentonite, sodium hydroxymethyl cellulose and xanthan gum, the disintegrating agent is one or two of lignocellulose, sodium hydroxymethyl starch and alginic acid, and the disintegration assistant is one or two of sodium ascorbate and sodium citrate.
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