CN110818094B - Method for restoring eutrophic water body - Google Patents
Method for restoring eutrophic water body Download PDFInfo
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- CN110818094B CN110818094B CN201911124645.5A CN201911124645A CN110818094B CN 110818094 B CN110818094 B CN 110818094B CN 201911124645 A CN201911124645 A CN 201911124645A CN 110818094 B CN110818094 B CN 110818094B
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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Abstract
The invention discloses a method for restoring eutrophic water, which comprises the following steps: firstly, preparing a eutrophic water body remediation medicament by using water, an oxygen release agent, biomass carbon powder, a composite microbial inoculum, an alkaline agent and a binder; secondly, the medicament is applied to eutrophic water body. The method for restoring the eutrophic water body has the advantages of high removal rate, low cost, environmental protection and the like, and can efficiently and durably restore the eutrophic water body.
Description
Technical Field
The invention belongs to the field of water environment restoration, and particularly relates to a method for restoring eutrophic water body.
Background
With the rapid development of industry and agriculture, a large amount of industrial wastewater containing nitrogen and phosphorus, domestic sewage and even untreated sewage are directly discharged into a river channel, so that part of the river has eutrophication. The eutrophication of water body is caused by excessive proliferation of algae, reduction of dissolved oxygen content in water body, mass death of aquatic organisms and deterioration of water quality due to overproof nutrient substances (such as nitrogen, phosphorus elements and the like) in the water body. The eutrophic water body not only causes the damage of the river ecological system, but also influences the production and life of people, so that the eutrophic water body restoration becomes a water environment problem to be solved urgently in the current urban river pollution problem of China.
The core of eutrophic water body restoration is to reduce the concentration of nitrogen and phosphorus elements in the water body and increase the concentration of dissolved oxygen in the water body. The method for restoring the eutrophic water body at the present stage comprises the following steps: the method has the advantages of external aid blocking, internal source control, water purification, ecological restoration and the like, but most methods have limited restoration effect on the eutrophic water body, the treatment time is long, the restoration mode is complex, special mechanical addition is needed, a large amount of financial and material resources are consumed, the problem of eutrophic water body pollution cannot be thoroughly solved, the treated water body is easy to rebound, and the eutrophication phenomenon reappears. Therefore, there is still a need in the art to develop better methods for treating eutrophic water bodies.
Disclosure of Invention
In order to solve the problems of the prior art and overcome the defects of the prior art, the invention aims to provide a low-cost and environment-friendly eutrophic water body remediation method capable of fundamentally remedying the eutrophic water body, and aims to efficiently and durably remediate the eutrophic water body. Through a large number of experiments, the inventor unexpectedly discovers that a good repairing effect on the eutrophic water body can be obtained by adopting the combination of the oxygen release agent, the biomass carbon powder, the composite microbial agent, the alkaline agent, the binder and the like in a specific proportion.
Based on the discovery, the invention provides a method for restoring eutrophic water, which comprises the following specific steps:
the eutrophic water body remediation agent is prepared from water, an oxygen release agent, biomass carbon powder, a composite microbial agent, an alkaline agent and a binder, wherein the agent comprises the following components in percentage by weight: 10-15% of water, 10-30% of oxygen release agent, 20-30% of biomass carbon powder, 10-20% of composite microbial inoculum, 5-10% of alkaline agent and 20-40% of binder;
secondly, the agent is applied to eutrophic water.
Preferably, in the repairing method of the present invention, the oxygen releasing agent is selected from one or a mixture of calcium peroxide, magnesium peroxide, sodium peroxide and potassium peroxide.
Preferably, in the repairing method of the present invention, the biomass carbon powder is obtained by: crushing a mixture of one or more raw materials selected from peanut shells, straws, corncobs, bagasse, wood chips and crushed barks by a crusher, sieving by a sieve of 20-80 meshes to obtain biomass carbon powder, soaking the biomass carbon powder in a 2-4% NaOH solution for 10-24 hours, washing the biomass carbon powder to be neutral by deionized water after soaking, and naturally drying the biomass carbon powder to constant weight.
Preferably, in the repairing method of the present invention, the binder is ordinary portland cement, and the ordinary portland cement consists of the following components in parts by mass: 40 to 60 percent of tricalcium silicate, 15 to 35 percent of dicalcium silicate, 7 to 15 percent of tricalcium aluminate and 10 to 18 percent of tetracalcium aluminoferrite.
Preferably, in the repairing method of the present invention, the alkaline agent is selected from calcium oxide, calcium hydroxide, calcium carbonate or a mixture thereof.
Preferably, in the repairing method of the present invention, the complex microbial inoculum is obtained by the following method: collecting 40mL of a target eutrophic water sediment sample, adding the sample into 100mL of a liquid culture medium, uniformly mixing, culturing for 3 days under the conditions of darkness, 30 ℃ temperature, 4-5 mg/L of dissolved oxygen and 160r/min of shaking table rotation speed, adding 1mL of the obtained enrichment culture into a fresh aerobic denitrifying bacteria enrichment culture medium, adding 4mL of 4-6% nitrate solution into the culture medium every 3-5 days, and selecting a purified strain without abnormal bacterial colonies after 5 times of enrichment culture; coating a purified strain on an aerobic denitrification flat plate separation solid culture medium, culturing at a constant temperature of 30 ℃ for 10 days, selecting a single colony as aerobic denitrifying bacterium, inoculating the obtained aerobic denitrifying bacterium to an amplification culture medium, performing amplification culture in a shaking culture box at a temperature of 25-30 ℃ and a speed of 200r/min for 3-5 days to obtain a bacterial liquid, adopting sterile vermiculite powder as an adsorption carrier, mixing the adsorption carrier and the bacterial liquid obtained after the amplification culture according to a weight ratio of (1-3) to 1, drying the mixture for 24 hours at a temperature of 35-40 ℃, and cooling to be close to room temperature by cold air; and crushing and grinding the obtained dried powder to obtain the solid powdery composite microbial inoculum.
In a preferred embodiment, the eutrophic water body restoration method of the present invention comprises the following steps: uniformly mixing an oxygen release agent, biomass carbon powder, a composite microbial agent, an alkaline agent and a binder, adding water in a granulator, rolling to spherical particles, placing the obtained spherical particle agent in a concrete curing box for curing, and then applying the agent to a water body.
In a preferred embodiment, the particle size of the spherulite medicament in the eutrophic water body remediation method is 0.3 cm-3 cm, the curing condition of the concrete curing box is 20 +/-2 ℃, the humidity is 95%, and the curing time is 5 days.
As known to those skilled in the art, eutrophication of water bodies refers to a phenomenon of water pollution caused by excessive nutrient content such as N, P in water bodies. More preferably, the method of the present invention is particularly suitable for eutrophic water bodies that meet the following criteria: sampling the water body to be investigated, measuring related indexes, and determining that the water body is eutrophic water body if the Total Nitrogen (TN) content is more than 0.5ppm, the Total Phosphorus (TP) content is more than 0.05ppm, the oxidation-reduction potential (ORP) of the overlying water body is less than 0mv, and the Dissolved Oxygen (DO) content is less than 4 ppm.
The composite microbial inoculum in the invention is preferably aerobic denitrifying bacteria.
Preferably, in the method of the present invention, the enrichment medium for aerobic denitrifying bacteria has the following composition: 0.5g of solid sodium acetate; potassium nitrate 0.05 g; 0.5g of disodium hydrogen phosphate heptahydrate; sodium nitrite 0.01g, magnesium sulfate heptahydrate 0.1 g; 18g of agar; the pH value is 7-7.5; the aerobic denitrification flat plate separation solid culture medium comprises the following components: 4.72g of sodium succinate, 0.01g of sodium nitrite, 1.5g of potassium dihydrogen phosphate, 7.9g of disodium hydrogen phosphate, 5g of casamino acid, 0.1g of magnesium sulfate heptahydrate and 1mL of trace element solution, wherein the pH value is 7-7.5; the expansion medium had the following composition: 5g of ammonium sulfate, 0.04g of ferrous sulfate heptahydrate, 0.5g of magnesium sulfate heptahydrate, 2g of sodium chloride, 0.01g of manganese sulfate tetrahydrate, 1g of dipotassium hydrogen phosphate, 5g of calcium chloride and 250mL of eutrophic water body, and deionized water is added to the eutrophic water body to reach the constant volume of 1000 mL.
Compared with the prior art, the invention has the following beneficial effects:
(1) the eutrophic water body is often high in nitrogen-phosphorus proportion and relatively low in carbon content, so that microorganisms are inhibited from absorbing and utilizing nitrogen and phosphorus to a certain extent. The inventor finds that compared with a treatment mode of directly adding liquid or solid carbon source into the water body, the eutrophic water body remediation agent used in the method can slowly release the carbon source, so that the microorganisms utilize nitrogen and phosphorus elements under the aerobic condition according to the ratio of C to N to P being 100 to 5 to 1, thereby preventing the carbon source from directly losing after entering the water body and also preventing the risk of secondary pollution to the water body, and continuously playing a role in remediation on the eutrophic water body;
(2) the eutrophic water body remediation agent used in the method has a large dead weight, and can directly sink to the water bottom after being thrown into the water body, so that the eutrophic water body remediation agent is convenient to throw;
(3) the oxygen release agent can improve the concentration of dissolved oxygen in the water body, improve the low-oxygen environment of the eutrophic water body and facilitate the microorganisms to quickly remove nitrogen and phosphorus elements in an aerobic state; in addition, the oxygen release agent can also improve the oxidation-reduction potential in the water body and inhibit the formation of fishy smell of the eutrophic water body;
(4) the eutrophic water body remediation agent used in the method disclosed by the invention contains biomass carbon powder, so that the porosity and the specific surface area are increased, microorganisms are easier to attach and fix on the eutrophic water body, the removal efficiency of nutrient elements in the water body is improved, and the resource recycling can be realized;
(5) the composite microbial inoculum used in the method preferably contains indigenous microorganisms, namely aerobic denitrifying bacteria, which are extracted from eutrophic water sediments and are treated, has good adaptability to the environment, does not impact bacterial colonies in the water, can realize synchronous nitrification and denitrification under the aerobic condition, and increases the efficiency of water denitrification;
(6) we have surprisingly found that the remediation process of the present invention comprises an alkaline agent, which is more effective in removing phosphorus from a body of water than a pharmaceutical agent that does not contain an alkaline agent;
(7) the raw materials used in the method are all environment-friendly materials, and the method has the characteristics of safety, high efficiency, easy storage, low cost, simple operation and the like, and can avoid water eutrophication.
Drawings
Figure 1 shows the oxygen and biomass char release per mass of material in example 3.
Detailed Description
In order to make the present invention more clearly understood by those skilled in the art, the technical solutions of the present invention are further explained below with reference to the drawings and specific examples and comparative examples. It should be understood that these examples and comparative examples are only for illustrating the present invention and are not to be construed as limiting the scope of the present invention, and those skilled in the art can make modifications, substitutions, improvements, etc. after reading the present invention, and these modifications, substitutions and improvements should be included in the scope of the present invention.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the invention. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of the stated features, steps, operations, and/or combinations thereof, unless the context clearly indicates otherwise.
In the present invention, the proportions of the components mentioned are all based on mass ratio or weight ratio.
Example 1
The medicament comprises the following components:
the eutrophic water body remediation agent comprises the following components in parts by mass: 10% of water, 20% of calcium peroxide, 20% of biomass carbon powder, 20% of a composite microbial inoculum, 5% of calcium hydroxide, 5% of calcium oxide and 20% of ordinary portland cement (the ordinary portland cement consists of tricalcium silicate, dicalcium silicate, tricalcium aluminate and tetracalcium aluminoferrite, and the mass ratio of the ordinary portland cement to the dicalcium silicate is 10:5:3: 2).
The biomass carbon powder is prepared by the following method:
crushing reed straws by a crusher, sieving the crushed reed straws by a sieve of 20-80 meshes to obtain biomass carbon powder, soaking the biomass carbon powder in a 2-4% NaOH solution, wherein the solid-to-liquid ratio is 1: 10-1: 20, soaking for 10-24 h, washing with deionized water to be neutral, and naturally drying to constant weight to obtain powdery biomass carbon powder.
The compound microbial inoculum is prepared by the following method:
collecting 40mL of a target eutrophic water sediment sample, adding 100mL of a liquid culture medium, uniformly mixing, culturing for 3 days under the conditions of darkness, 30 ℃ temperature, 4.5mg/L of dissolved oxygen and 160r/min of shaking table rotation speed, adding 1mL of enrichment culture into a fresh aerobic denitrifying bacteria enrichment culture medium, continuously supplying 4mL of 4-6% nitrate solution every 3-5 days, and gradually selecting purified strains without abnormal bacterial colonies after 5 times of enrichment culture. Coating the purified bacterial suspension on an aerobic denitrification flat plate separation solid culture medium, culturing at the constant temperature of 30 ℃ for 10 days, repeatedly selecting a single colony as aerobic denitrifying bacteria, inoculating the obtained aerobic nitrifying bacteria to an expanded culture medium, performing expanded culture in a shaking culture box at the temperature of 25-30 ℃ and at the speed of 200r/min for 3-5 days after inoculation, finally adopting sterile vermiculite powder as an adsorption carrier, mixing the adsorption carrier and bacterial liquid according to the weight ratio of (1-3): 1, finally drying the mixture for 24 hours at the temperature of 35-40 ℃, and finally cooling the dried product to the room temperature by cold air and removing the dried product; and (4) crushing and grinding the dried powder obtained in the last step to obtain the solid powdery composite microbial inoculum.
Preferably, in the preparation method of the complex microbial inoculum, the aerobic denitrifying bacteria enrichment medium has the following composition: 0.5g of solid sodium acetate; potassium nitrate 0.05 g; 0.5g of disodium hydrogen phosphate heptahydrate; sodium nitrite 0.01g, magnesium sulfate heptahydrate 0.1 g; 18g of agar; the pH value is 7-7.5; the aerobic denitrification flat plate separation solid culture medium comprises the following components: 4.72g of sodium succinate, 0.01g of sodium nitrite, 1.5g of potassium dihydrogen phosphate, 7.9g of disodium hydrogen phosphate, 5g of casamino acid, 0.1g of magnesium sulfate heptahydrate and 1mL of trace element solution, wherein the pH value is 7-7.5; the expansion medium had the following composition: 5g of ammonium sulfate, 0.04g of ferrous sulfate heptahydrate, 0.5g of magnesium sulfate heptahydrate, 2g of sodium chloride, 0.01g of manganese sulfate tetrahydrate, 1g of dipotassium hydrogen phosphate, 5g of calcium chloride and 250mL of eutrophic water body, and deionized water is added to the eutrophic water body to reach the constant volume of 1000 mL.
The preparation method comprises the following steps:
the eutrophic water body remediation agent is prepared by the following steps:
the components are taken according to the mass percentage, are uniformly mixed, roll to a spherical shape of 0.3-3 cm in a granulator, and are put into a concrete curing box with the temperature of 20 +/-2 ℃ and the humidity of 95% for curing for 5 days, so that the eutrophic water body remediation agent can be prepared.
The application method comprises the following steps:
the method is applied to the simulated eutrophic water body restoration, and comprises the following specific steps: by surveying the target water body (the white lake water body), the pollution factors of main eutrophication are determined to be nitrogen and phosphorus, wherein the COD index does not exceed the standard. 1L of eutrophic water is selected and placed in a 1L beaker for beaker experiment, the dosage of the eutrophic water is 20g/L, the total nitrogen concentration is reduced from 4.52mg/L to 1.08mg/L after the dosage is 7 days, the removal rate can reach 76.11 percent, the total phosphorus concentration is reduced from 0.830mg/L to 0.443mg/L, the removal rate can reach 53.32 percent, the dissolved oxygen is increased from 1.2mg/L to 4.6mg/L, and the oxidation-reduction potential is increased from-67.70 mv to 13.22 mv.
Example 2 (actual engineering test)
The in-situ remediation test case of the eutrophic water body by the white lake comprises the following steps:
background: due to rapid development of industry and agriculture and dramatic increase of population in the white lake basin, the white lake water area is polluted, and the water body eutrophication is serious.
Step one (investigating and determining eutrophic water body): a square water area with the length and the width of 50m is selected at the position close to the bank of the white lake water area for water sample determination, the obtained data are shown in the following table 1, and it can be seen that the total phosphorus, the total nitrogen, the oxidation-reduction potential value (ORP) of the overlying water body and the dissolved oxygen at the point do not accord with the standard of the normal water body, so the water area surrounded by the square is the eutrophic water body.
Table 1 data table for water quality at a certain point of the white lake:
| total nitrogen (mg/L) | Total phosphorus (mg/L) | Upper Water ORP (mv) | Dissolved oxygen (mg/L) |
| 4.52 | 0.83 | -67.7 | 1.2 |
Step two (preparation of the eutrophic water body remediation agent of the invention): the eutrophic water body remediation agent of the invention comprises the following components: the composition comprises the following components in parts by mass: 5% of water, 25% of calcium peroxide, 20% of biomass carbon powder, 25% of a compound microbial inoculum, 3% of calcium hydroxide, 2% of calcium oxide and 20% of ordinary portland cement (the ordinary portland cement consists of tricalcium silicate, dicalcium silicate, tricalcium aluminate and tetracalcium aluminoferrite, and the mass ratio of the ordinary portland cement to the dicalcium silicate is 10:5:3: 2).
The biomass carbon powder is prepared by the following method:
crushing reed straws by a crusher, sieving the crushed reed straws by a sieve of 20-80 meshes to obtain biomass carbon powder, soaking the biomass carbon powder in a 2-4% NaOH solution, wherein the solid-to-liquid ratio is 1: 10-1: 20, soaking for 10-24 hours, washing the biomass charcoal powder with deionized water to be neutral after soaking, and naturally drying the biomass charcoal powder to be constant in weight to obtain powdery biomass charcoal powder.
The compound microbial inoculum is prepared by the following method:
collecting 40mL of a target eutrophic water sediment sample, adding the sample into 100mL of a liquid culture medium, uniformly mixing, culturing for 3 days under the conditions of darkness, 30 ℃ temperature, 4.5mg/L of dissolved oxygen and 160r/min of shaking table rotation speed, adding 1mL of the obtained enrichment culture into a fresh aerobic denitrifying bacteria enrichment culture medium, adding 4mL of 4-6% nitrate solution into the culture medium every 3-5 days, and selecting a purified strain without abnormal bacterial colonies after 5 times of enrichment culture; coating purified bacteria on an aerobic denitrification flat plate separation solid culture medium, culturing at the constant temperature of 30 ℃ for 10 days, selecting a single colony as aerobic denitrification bacteria, inoculating the obtained aerobic nitrification bacteria to an amplification culture medium, performing amplification culture in a shaking culture box at the temperature of 25-30 ℃ and at the speed of 200r/min for 3-5 days to obtain a bacterial liquid, adopting sterile vermiculite powder as an adsorption carrier, mixing the adsorption carrier and the bacterial liquid obtained after the amplification culture according to the weight ratio of (1-3): 1, drying the mixture for 24 hours at the temperature of 35-40 ℃, and cooling to the room temperature by cold air; and crushing and grinding the obtained dried powder to obtain the solid powdery composite microbial inoculum.
Preferably, in the preparation method of the complex microbial inoculum, the aerobic denitrifying bacteria enrichment medium has the following composition: 0.5g of solid sodium acetate; potassium nitrate 0.05 g; 0.5g of disodium hydrogen phosphate heptahydrate; sodium nitrite 0.01g, magnesium sulfate heptahydrate 0.1 g; 18g of agar; the pH value is 7-7.5; the aerobic denitrification flat plate separation solid culture medium comprises the following components: 4.72g of sodium succinate, 0.01g of sodium nitrite, 1.5g of potassium dihydrogen phosphate, 7.9g of disodium hydrogen phosphate, 5g of casamino acid, 0.1g of magnesium sulfate heptahydrate and 1mL of trace element solution, wherein the pH value is 7-7.5; the expansion medium had the following composition: 5g of ammonium sulfate, 0.04g of ferrous sulfate heptahydrate, 0.5g of magnesium sulfate heptahydrate, 2g of sodium chloride, 0.01g of manganese sulfate tetrahydrate, 1g of dipotassium hydrogen phosphate, 5g of calcium chloride and 250mL of eutrophic water body, and deionized water is added to the eutrophic water body to reach the constant volume of 1000 mL.
The preparation method comprises the following steps:
the eutrophic water body remediation agent is prepared by the following steps:
the components are taken according to the mass percentage, are uniformly mixed, roll to a spherical shape of 0.3-3 cm in a granulator, and are put into a concrete curing box with the temperature of 20 +/-2 ℃ and the humidity of 95% for curing for 5 days, so that the eutrophic water body remediation agent can be prepared.
Step three (medication for eutrophic water body): the area around the square was cut off by dyke, and it was found from example 1 that the laboratory beaker test gave a good effect when the amount of drug was 20g/L, but now, considering the actual engineering test, the amount of drug was increased and the amount of drug applied to the river was 40g/L, and after the application of the drug, the water quality was changed as shown in Table 2, and as can be seen from Table 2, the removal rate of total nitrogen was close to 50% after day 15 when the drug was used, the removal rate of total phosphorus was over 90%, the ORP (oxidation reduction potential) was increased by 60% on day 15, and the level of dissolved oxygen could be improved from the oxygen deficiency level within day 15.
Table 2 in-situ remediation of water quality tables for white lake eutrophic waters:
example 3
Hydrostatic leaching experiment:
the eutrophic water restoration agent prepared in example 1 was used to perform a still water leaching experiment, and the eutrophic water restoration agent was placed in a 500ml conical flask, and ultrapure water was used as a leaching agent, which had a COD of 0, and an appropriate amount of sodium sulfite was used as an oxygen quencher for the ultrapure water, so that the dissolved oxygen amount was 0. Adding the medicament according to the volume (cm3) of the leaching agent and the geometric surface area (cm2) of the sample being 10 +/-0.2 cm, and sealing the conical flask by using a sealing film immediately after the medicament is added. Samples were taken from the flasks at regular intervals in 7 portions and immediately transferred to another flask containing fresh leachant, and the original flask was immediately subjected to water sampling. The lixiviant change times are shown in table 2.
Example 3 oxygen and biomass char release per unit mass of material (mg/L) release rates up to 30d are shown in figure 1: the oxygen and biomass charcoal release amounts are 45.94% and 59.56%, respectively. Specific release amounts are shown in table 4.
TABLE 3 Leaching agent Change time
TABLE 4 hydrostatic leaching Release Table
Comparative example 1
Referring to the mixture ratio of example 1, the complex agent of the comparative example is different from the repairing agent of example 1 only in that the complex agent is not contained in the agent of the comparative example, the complex agent is replaced by ordinary portland cement (the ordinary portland cement consists of tricalcium silicate, dicalcium silicate, tricalcium aluminate and tetracalcium aluminoferrite in a mass ratio of 10:5:3:2), and the rest components and the preparation process are unchanged.
The eutrophic water body remediation agent prepared by the comparative example is applied to the remediation of the actual polluted eutrophic water body and bottom mud, and comprises the following specific steps: by surveying the white lake water body, the pollution factors of main eutrophication are determined to be nitrogen and phosphorus, and COD index does not exceed standard. 1L of eutrophic water is selected and placed in a 1L beaker for beaker experiment, and the dosage of the eutrophic water medicament is 20 g/L. The total nitrogen concentration is reduced from 4.52mg/L to 4.05mg/L, and the removal rate is reduced by more than 60% compared with example 1, which is determined again after 7 days of administration; the total phosphorus is reduced from 0.830g/L to 0.782mg/L, and the removal rate is reduced by more than 40% compared with that of example 1; the oxidation-reduction potential is increased from-67.70 mv to-39.22 mv; the dissolved oxygen increased from 1.2mg/L to 2.3mg/L, and the increase rate was reduced by 26.08% as compared with example 1.
Comparative example 2
Referring to the mixture ratio of example 1, the repairing agent prepared in the comparative example is different from the repairing agent prepared in example 1 only in that the repairing agent prepared in the comparative example does not contain an oxygen release agent, the common portland cement (the common portland cement consists of tricalcium silicate, dicalcium silicate, tricalcium aluminate and tetracalcium aluminoferrite, the mass ratio of the common portland cement to the dicalcium aluminate is 10:5:3:2) is used for replacing the composite microbial inoculum, and the rest components and the preparation process are unchanged.
The eutrophic water body remediation agent prepared by the comparative example is applied to the remediation of the actual polluted eutrophic water body and bottom mud, and comprises the following specific steps: by surveying the white lake water body, the pollution factors of main eutrophication are determined to be nitrogen and phosphorus, and COD index does not exceed standard. 1L of eutrophic water is selected and placed in a 1L beaker for beaker experiment, and the dosage of the eutrophic water medicament is 20 g/L. The total nitrogen concentration is reduced from 4.52mg/L to 4.36mg/L, and the removal rate is reduced by more than 70% compared with example 1, which is determined again after 7 days of administration; the total phosphorus is reduced from 0.830g/L to 0.795mg/L, and the removal rate is reduced by more than 40 percent compared with that of example 1; the oxidation-reduction potential is increased from-67.70 mv to-65.98 mv, and basically has no change; the dissolved oxygen was reduced from 1.2mg/L to 0.87 mg/L.
Comparative example 3
Referring to the mixture ratio of example 1, the repairing agent prepared in the present comparative example is different from the repairing agent of example 3 only in that the agent of the present comparative example does not contain an alkali agent, the alkali agent is replaced by ordinary portland cement (the ordinary portland cement consists of tricalcium silicate: dicalcium silicate: tricalcium aluminate: tetracalcium aluminoferrite in a mass ratio of 10:5:3:2), and the rest of the components and the preparation process are unchanged.
The eutrophic water body remediation agent prepared by the comparative example is applied to the remediation of the actual polluted eutrophic water body and bottom mud, and comprises the following specific steps: by surveying the white lake water body, the pollution factors of main eutrophication are determined to be nitrogen and phosphorus, and COD index does not exceed standard. 1L of eutrophic water is selected and placed in a 1L beaker for beaker experiment, and the dosage of the eutrophic water medicament is 20 g/L. The total nitrogen concentration is reduced from 4.52mg/L to 2.95mg/L, and the removal rate is reduced by more than 30% compared with example 1, which is determined again after 7 days of administration; the total phosphorus is reduced from 0.830g/L to 0.576mg/L, and the removal rate is reduced by more than 20 percent compared with that of example 1; the oxidation-reduction potential is increased from-67.70 mv to 12.78mv, and basically has no change; the dissolved oxygen increased from 1.2mg/L to 4.4mg/L with essentially no change.
The embodiment and the comparative example show that the eutrophic water body remediation agent has the advantages of obviously better effect, easily obtained raw materials, environmental friendliness, convenient use and low cost.
From the results, the eutrophic water body remediation agent has the effects of slowly releasing oxygen and a carbon source, has a good slow release effect, is controllable in the amount of the agent added into a water body, can improve the oxidation-reduction potential of eutrophication by the released oxygen, and can improve the dissolved oxygen content of a water environment, and the slowly released carbon source can stimulate microorganisms to absorb and utilize nitrogen and phosphorus in the water body according to the proportion of C to N to P of 100 to 5 to 1; the aerobic denitrifying bacteria in the composite microbial inoculum also reduce the concentration of nitrogen and phosphorus in the water body through the action of respiration and anabolism. The eutrophic water body remediation agent can efficiently and continuously restore the eutrophic water body and avoid the eutrophication phenomenon from rebounding.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (7)
1. The method for restoring the eutrophic water body is characterized by comprising the following specific steps:
the eutrophic water body remediation agent is prepared from water, an oxygen release agent, biomass carbon powder, a composite microbial agent, an alkaline agent and a binder, wherein the agent comprises the following components in percentage by weight: 10-15% of water, 10-30% of oxygen release agent, 20-30% of biomass carbon powder, 10-20% of composite microbial inoculum, 5-10% of alkaline agent and 20-40% of binder; the compound microbial inoculum is obtained by the following method: collecting 40mL of a target eutrophic water sediment sample, adding the sample into 100mL of a liquid culture medium, uniformly mixing, culturing for 3 days under the conditions of darkness, 30 ℃ temperature, 4-5 mg/L of dissolved oxygen and 160r/min of shaking table rotation speed, adding 1mL of the obtained enrichment culture into a fresh aerobic denitrifying bacteria enrichment culture medium, adding 4mL of 4-6% nitrate solution into the culture medium every 3-5 days, and selecting a purified strain without abnormal bacterial colonies after 5 times of enrichment culture; coating a purified strain on an aerobic denitrification flat plate separation solid culture medium, culturing at a constant temperature of 30 ℃ for 10 days, selecting a single colony as aerobic denitrification bacteria, inoculating the obtained aerobic denitrification bacteria to an amplification culture medium, performing amplification culture in a shaking incubator at a temperature of 25-30 ℃ and a speed of 200r/min for 3-5 days to obtain a bacterial liquid, adopting sterile vermiculite powder as an adsorption carrier, mixing the adsorption carrier and the bacterial liquid obtained after the amplification culture according to a weight ratio of (1-3) to 1, drying the mixture for 24 hours at a temperature of 35-40 ℃, and cooling to be close to room temperature by cold air; grinding the obtained dry powder to obtain a solid powdery compound microbial inoculum;
secondly, the agent is applied to eutrophic water.
2. The method for remediating an eutrophic water body according to claim 1, wherein the oxygen releasing agent is one or a mixture of two or more of calcium peroxide, magnesium peroxide, sodium peroxide and potassium peroxide.
3. The method for remediating the eutrophicated water body according to claim 1, wherein the biomass carbon powder is obtained by the following method: crushing one or more than two of raw materials selected from peanut shells, straws, corncobs, bagasse, wood chips and crushed barks by a crusher, sieving by a sieve of 20-80 meshes to obtain biomass carbon powder, soaking the biomass carbon powder in a 2-4% NaOH solution for 10-24 h, washing the biomass carbon powder to be neutral by deionized water after soaking, and naturally drying the biomass carbon powder to be constant weight.
4. The method for remediating an eutrophicated water body according to claim 1, wherein the binder is ordinary portland cement, and the ordinary portland cement comprises the following components in percentage by mass: 40-60% of tricalcium silicate, 15-35% of dicalcium silicate, 7-15% of tricalcium aluminate and 10-18% of tetracalcium aluminoferrite.
5. The method for remediating an eutrophic water body according to claim 1, wherein the alkaline agent is selected from the group consisting of calcium oxide, calcium hydroxide, calcium carbonate, and mixtures thereof.
6. The eutrophic water body restoration method according to any one of claims 1 to 5, wherein the preparation method of the agent for restoring the eutrophic water body comprises the following steps: uniformly mixing an oxygen release agent, biomass carbon powder, a composite microbial agent, an alkaline agent and a binder, adding water in a granulator, rolling to spherical particles, placing the obtained spherical particle agent in a concrete curing box for curing, and then applying the agent to a water body.
7. The method for remedying the eutrophicated water body according to claim 6, wherein the grain size of the spherical grain agent is 0.3cm to 3cm, the curing condition of the concrete curing box is 20 ± 2 ℃, the humidity is 95%, and the curing time is 5 days.
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