CN102146342A - Halophilic bacterial agent and preparation method thereof as well as biological treatment system fixed with bacterial agent and application thereof - Google Patents
Halophilic bacterial agent and preparation method thereof as well as biological treatment system fixed with bacterial agent and application thereof Download PDFInfo
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Abstract
The invention discloses a halophilic bacterial agent and a preparation method thereof as well as a biological treatment system fixed with the bacterial agent and application thereof. In the invention, strains are screened from sewage, sludge and soil to be prepared into a bacterial agent, wherein the bacterial agent comprises four strains including Providenciasp. CJ-4, Oceanobcillussp. CJ-10, Brevibacterium epidermidis CJ-12 and Halomonassp. CJ-13. The invention also provides the biological treatment system fixed with the bacterial agent and a method for treating waste water by using the same. The bacterial agent disclosed by the invention has stronger degradation capability on phenolic wastewater, can adapt to high-salt environments and has very good degradation effects under an environment with the salt content of 2-25% and the phenol concentration of 2500 mg/l; and compared with the traditional active sludge method, the bacterial agent has obvious advantages in the performance of degrading phenolic compounds, and the degradation effect on the phenolic compounds in water can reach more than 99.9%.
Description
Technical field
The present invention relates to a kind of halophilic bacterium and the application in handling waste water thereof, be specifically related to a kind of can efficient degradation waste water in the halophilic bacterium and the application in handling the high phenol waste water of high salt thereof of phenol, belong to microorganism and technical field of waste water processing.
Background technology
Phenolic compound has the special fragrance smell, all is slightly acidic, and is easily oxidized in environment.Because the purposes of phenol is very extensive, prevents the work of its pollution also very difficult.Phenolic wastewater is one of trade effluent that harm is big in the world today, pollution range is wide, is the important source that water pollutes in the environment.Phenolic wastewater is mainly from industrial sector and petroleum cracking system ethene, synthesizing phenol, tynex, synthol, organic pesticide and resol production processes such as coke-oven plant (especially low temperature coke making by indigenous methods), producer gas plant, petroleum chemical plant, insulating material factorys.For example produce coke, coal gas, 2, the waste water that the 4-D pesticide mother liquor produces contains phenol concentration up to 2000-12000mg/L.Phenolic wastewater mainly contains the phenolic group compound, as phenol, cresols and nitrocresol etc.If these waste water are without processing, but direct then atmosphere pollution, water, soil and food of discharging, irrigated farmland.The water that is polluted by phenol in the environment, after being absorbed by the body, by function of detoxification in the body, can make its most of forfeiture toxicity, and excrete with urine, if when entering the intravital amount of people above normal human's function of detoxification, exceeding part can accumulate in vivo in each organs and tissues, cause chronic poisoning, in various degree giddy, headache, fash, skin itching, spirit uneasiness, anaemia and various neurological symptom and chronic digestible road symptoms such as poor appetite, dysphagia, hydrostomia, vomiting and diarrhoea occur.This chronic poisoning generally can not stay sequela through suitably treating.Therefore the processing to phenolic wastewater is even more important.
Treatment process to phenolic wastewater generally is divided into physico-chemical process, chemical method and biochemical process three classes at present.Biochemical process is owing to have advantage such as efficient, energy-conservation, non-secondary pollution, is considered to a kind of method of purifying liquid waste of tool development prospect.Adopting the prerequisite of biochemical process Phenol-Containing Wastewater Treatment is to screen the microorganism that phenolic compound is had good degradation effect, also has very high salt concn outward owing to contain phenolic compound in the phenolic wastewater that some industries are discharged, produce the waste water that nitrophenols produces as chemical industry, under this hypersaline environment, as long as salt concn＞3000mg/L microbic activity will be suppressed, therefore research has the microorganism of good degradation capability to have great significance and wide application prospect for the processing of carrying out the high salt concentration phenolic wastewater to phenol under hypersaline environment, and this type of research rests on theory substantially, and industrial applications seldom.
Summary of the invention
In order to make biochemical process in the phenolic wastewater of hypersaline environment, the better application prospect can be arranged, the invention provides a kind of suitable hypersaline environment, can efficient degradation waste water in aldehydes matter the halophilic bacterium microbial inoculum and prepare the method for this halophilic bacterium microbial inoculum.
The present invention also provides biological treatment system and the high phenol wastewater treatment method of high salt that is fixed with this halophilic bacterium microbial inoculum, waste water COD after biological treatment system is handled is lower than 200mg/L, further adopt magnetism macroporous ion exchange resin to adsorb, absorption back waste water TOC is lower than 20mg/L, the phenol emission concentration is lower than 0.2mg/L, the final waste water of handling can satisfy chlor-alkali starting material needs by chlor-alkali is once refining with secondary refining, perhaps by reclaiming solid salt after the airing of saltern, basic zero phenol of realizing discharges.
Below the present invention will be described in detail:
Comprise bacterial classification in 4 in the halophilic bacterium microbial inoculum of the present invention, they are respectively: Providence (Providencia sp.) CJ-4, be deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC) on November 12nd, 2010, preserving number is CGMCC NO.4327; Oceanobacillus sp. CJ-10 is deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC) on November 12nd, 2010, and preserving number is CGMCC NO.4328; Brevibacterium epidermidis (Brevibacterium epidermidis) CJ-12 is deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC) on November 12nd, 2010, and preserving number is CGMCC NO.4329; Salt Zymomonas mobilis (Halomonas sp.) CJ-13 is deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC) on November 12nd, 2010, and preserving number is CGMCC NO.4330.
Bacterial classification of the present invention obtains by screening and domestication, and following several stages is specifically arranged:
One, the bacterial classification that screening can the efficient degradation aldehydes matter
1, may contact the sewage at phenolic wastewater place or mud as the bacterium source with plant effluent pond, discharge outlet and the water drain etc. that produce phenolic wastewater.
2, the yeast extract protein culture medium of bacterium source with the phenolic wastewater preparation screened, filter out well-grown bacterial classification; Screening method is: medium salts content is adjusted to 2-25%, inoculum size with 10% inserts the bacterium source in substratum then, pick out the bacterium source of well-grown bacterial classification as the efficient Phenol-Containing Wastewater Treatment of screening, obtain 4 kinds of well-grown halophilic bacterium bacterial classifications by present method, each bacterial classification is carried out stored refrigerated respectively under 4 ℃.
The yeast extract protein culture medium consists of: phenol content is 100mg/L in the waste water, salts contg 2-25wt%, contain yeast extract 10.0g in every liter of waste water, peptone 7.5g, Trisodium Citrate 3.0g, Repone K 2.0g, sal epsom 20.0g, to 8.5-9.5, and pass through high pressure steam sterilization with 30% liquid adjusting PH with base.
3, cryopreserved halophilic bacterium bacterial classification is inoculated in the yeast extract protein culture medium (nutrient media components is the same) by 10% inoculum size, place constant temperature shaking table shaking culture, culture condition: temperature is 32
oC, rotating speed are under the rotating speed of 120rpm, and continuous oscillation was cultivated 72 hours.
4, with the substratum centrifugation after each enrichment culture, get the DIFFERENT WET thalline, wet thallus joins in the 250mL triangular flask that the 100mL inorganic medium is housed by the amount of 1g/100mL waste water, place the constant temperature culture shaking table, 32 ℃, the 120rpm rotating speed is cultivated 48h down, the removal effect of COD and aldehydes matter in the detection substratum, screening COD degradation rate is more than 50%, and the aldehydes matter clearance carries out stored refrigerated respectively at the halophilic bacterium bacterial classification of the efficient degradation aldehydes matter more than 90% under 4 ℃.
Used inorganic medium is 2, the inorganic medium of 4-D phenolic wastewater preparation, component is: potassium primary phosphate 0.23-0.26g/L, dipotassium hydrogen phosphate concentration is 0.23-0.26g/L, sal epsom 1.9-2.1g/L, calcium chloride 0.18-0.21g/L, iron(ic) chloride 0.09-0.11g/L, ammonium chloride 0.9-1.1g/L, containing phenol concentration in the waste water is that 0.1-2.5g/L, sodium chloride content are 2-25%.
5, the halophilic bacterium bacterial classification to 4 kinds of efficient degradation aldehydes matters filtering out dilutes coating respectively, isolates single bacterium, and each single bacterium is preserved down at 4 ℃.
Two, isolated 4 kinds of efficient phenol-degrading halophilic bacteria list bacterium are tamed respectively, determined the tolerance of bacterial classification phenol
(a) with 10% inoculum size a certain efficient phenol-degrading halophilic bacteria list bacterium is inoculated at first that to contain phenol be 2 of 100mg/L, in the 4-D phenolic wastewater, at 32 ℃, the 120rpm rotating speed was cultivated 72 hours down;
(b) more above-mentioned bacterial classification is inoculated into by 10% inoculum size that to contain phenol be 2 of 300mg/L, in the 4-D phenolic wastewater, at 32 ℃, the 120rpm rotating speed was cultivated 72 hours down;
(c) constantly improve the concentration of aldehydes matter according to above method, finally obtain the halophilic bacterium bacterial classification of this salt concn that the phenolic wastewater of aldehydes matter content below 2500mg/L can both degrade by continuous domestication.
The acclimation method of the bacterial classification of other salt concn is identical, with after the above-mentioned instructionization can the phenolic wastewater of degrading phenol substances content below 2500mg/L 4 kinds of bacterial classifications dilute coating respectively, isolate single bacterium, and each single bacterium preserved down at 4 ℃.These 4 kinds of single bacterium are CGMCCNO.4327, CGMCCNO.4328, CGMCCNO.4329, the CGMCCNO.4330 of preservation of the present invention, in use bacterial classification are carried out enlarged culturing, mix to use promptly to get the halophilic bacterium microbial inoculum.
Concrete technical scheme of the present invention is as follows:
A kind of halophilic bacterium microbial inoculum is characterized in that: comprise Providence (Providencia sp.) CJ-4 CGMCC NO.4327, Oceanobacillus sp.CJ-10 CGMCC NO.43287, brevibacterium epidermidis (Brevibacterium epidermidis) CJ-12 CGMCC NO.4329 and salt Zymomonas mobilis (Halomonas sp.) CJ-13 CGMCC NO.4330.
Above-mentioned halophilic bacterium microbial inoculum, the weight proportion of each component is in the microbial inoculum:
Providence (Providencia sp.) CJ-4 CGMCC NO.4327 4-10 part;
Oceanobacillus sp.CJ-10 CGMCC NO.4328 4-10 part;
Brevibacterium epidermidis (Brevibacterium epidermidis) CJ-12 CGMCC NO.4329 3-9 part;
Salt Zymomonas mobilis (Halomonas sp.) CJ-13 CGMCC NO.4330 4-10 part.
The preparation method of above-mentioned halophilic bacterium microbial inoculum is: each bacterial classification is linked in the substratum by 10% inoculum size respectively, under temperature 15-35 ℃, the condition of pH 8.5-9.5, dissolved oxygen 2-4mg/L, bacterial classification is carried out enlarged culturing 72-96h, after the cultivation each substratum centrifugation is got wet thallus, the gained wet thallus is mixed by proportioning promptly get halophilic bacterium microbial inoculum of the present invention; Used substratum is glucose sugar 9-11g/L, urea 1.0-1.2g/L, potassium primary phosphate 1.29-1.34g/L, sodium-chlor 2-25wt%.
The invention provides a kind of biological treatment system, comprise one-level anaerobic biofilter+secondary anaerobic biological filter+one-level aerobic biofilter+secondary aerobic biofilter, it is characterized in that: described one-level anaerobic biofilter, secondary anaerobic biological filter, one-level aerobic biofilter and secondary aerobic biofilter are connected successively, all be fixed with halophilic bacterium microbial inoculum of the present invention in one-level anaerobic biofilter, secondary anaerobic biological filter, one-level aerobic biofilter and the secondary aerobic biofilter, the fixed amount of halophilic bacterium microbial inoculum is 1-5g/L waste water.Described one-level, secondary be first, second the meaning.
In the above-mentioned biological treatment system, fixedly the used carrier of halophilic bacterium is haydite, volcanized rock, urethane floating stuffing, granulated active carbon or high temperature bamboo charcoal, and the set time is 24-48h.
In the above-mentioned biological treatment system, fixedly the used carrier of halophilic bacterium is preferably granulated active carbon.
The present invention also provides a kind of high salt high phenol wastewater treatment method, it is characterized in that may further comprise the steps at least:
(1) the saliferous phenolic wastewater enters preparing pool, and COD, N in the adjusting waste water and the content of P are adjusted to 2-25wt% with salts contg in the waste water simultaneously;
(2) waste water enters claim 4 or 5 described biological treatment system biodegrades, COD ﹤ 200mg/L in the processed waste water, phenol ﹤ 0.2mg/L;
(3) biological treatment back waste water further adopts magnetism macroporous ion exchange resin to adsorb, TOC ﹤ 20mg/L in the waste water of absorption back;
(4) waste water after the step (3) reclaims solid salt after entering the chlor-alkali process for refining or entering the saltern airing.
In the above-mentioned steps (1), add urea and regulate N content in the waste water, add KH
2PO
4Regulate P content in the waste water, the concentration ratio that makes COD:N:P in the waste water is 200:5:1.
In the above-mentioned steps (2), the firsts and seconds anaerobic biofilter wastewater treatment condition of biological treatment system is: pH 8.5-9.5, temperature 15-35 ℃, time 20-24h; One-level aerobic biofilter wastewater treatment condition is: pH 8.5-9.5, temperature 15-35 ℃, time 20-24h, dissolved oxygen 2-4mg/L; Wastewater treatment condition in two stage biological filter tank is: pH 8.5-9.5, temperature 15-35 ℃, time 20-24h, dissolved oxygen 2-4mg/L.
In the above-mentioned steps (3), biological treatment back wastewater pH 6-9, flow velocity is 15-30mL/min, adsorption temp is 25-35 ℃.
In the above-mentioned treatment process, phenol content is below 2500mg/L in the waste water, and COD is below 15000mg/L, and contained phenol is phenol, 2 in the waste water, 4-D class phenol, nitrophenols, 2,4, at least a in 6-trichlorophenol, adjacency pair two chlorophenols, cresols and the nitrocresol.
Advantage of the present invention:
(1) separating that domestication obtained can be at saliferous 2-25%, phenol concentration is to survive in the following phenolic wastewater of 2500mg/L, and be the halophilic bacterium agent of the efficient degradation phenolic wastewater of nutrition source with organism in phenol and the waste water, it falls in phenol and the phenolic wastewater other organic performances and traditional physico-chemical process+biological process and handles high salt and contain the organic high-concentration waste water of phenol and compare and have clear superiority.It is short, simple to operate that the present invention has technical process, is easy to advantages such as control and working cost are low.
(2) the present invention handle salt be divided into 2-25%, the dense investment degree of phenol be the following phenolic wastewater qualified discharge of 2500mg/L required best to throw the bacterium weight range be 1-5g/L, need not repeat to add after after once dropping into.
(3) the present invention utilizes halophilic bacterium processing saltiness to be the high-salt wastewater below the 2500mg/L for the phenol that contains of 2-25%, after the biochemical system processing that is fixed with the halophilic bacterium agent, can reach COD ﹤ 200mg/L, phenol ﹤ 0.2mg/L, then by magnetism macroporous resin degree of depth absorption, final outflow water TOC ﹤ 20mg/L, and be transported to Yancheng and evaporate brine, this technology has not only been carried out harmless treatment to containing the poisonous waste water of phenol, and can also reclaim salt in the waste water, the salt that reclaims can be used as the chlorine industry starting material, has realized waste water resource and utilization, and produces certain economic value.
(4) the present invention compares with the method (technique means such as distillation, burning) of conventional process high-salt wastewater, and working cost reduces greatly, and can not produce secondary pollution, can also produce the certain economic effect by reclaiming salt;
(5) the present invention can directly handle saliferous at 2-25% by biochemical system, contains the following phenolic wastewater of phenol 2500mg/L, thereby has avoided traditional biochemical method Phenol-Containing Wastewater Treatment drawback through just entering biochemical system after the extraction phenol earlier;
(6) the present invention can reach more than 99.9% aldehydes matter degradation effect in the water, and the wastewater treatment that can be in industrial sectors such as coke-oven plant, producer gas plant, petroleum chemical plant, insulating material factory and petroleum cracking system ethene, synthesizing phenol, tynex, synthetic dyestuff, organic pesticide and the resol production process provides technical guarantee.
Microbial preservation information
Providence (Providencia sp.) CJ-4 is deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC) on November 12nd, 2010, and preserving number is CGMCC NO.4327.
Oceanobacillus sp. CJ-10 is deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC) on November 12nd, 2010, and preserving number is CGMCC NO.4328.
Brevibacterium epidermidis (Brevibacterium epidermidis) CJ-12 is deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC) on November 12nd, 2010, and preserving number is CGMCC NO.4329.
Salt Zymomonas mobilis (Halomonas sp.) CJ-13 is deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC) on November 12nd, 2010, and preserving number is CGMCC NO.4330.
Description of drawings
Fig. 1 is the structural representation of biological treatment system of the present invention.
Fig. 2 halophilic bacterium agent domestication flow process.
Among the figure, 1, the one-level anaerobic biofilter, 2, the secondary anaerobic biological filter, 3, the one-level aerobic biofilter, 4, the secondary aerobic biofilter.
Embodiment
Below by specific embodiment the present invention is further set forth, should be understood that, following elaboration only is in order to explain the present invention, its particular content not to be limited.
Introduce the obtaining of bacterial classification of the present invention, domestication process below in detail.
One, obtains the degraded bacterial classification
1, with 2,4-D production plant is as the place of strain screening, and its bacterium source is selected from 2, and the wastewater disposal basin in the 4-D production plant, discharge outlet and water drain etc. may contact the sewage or the mud at phenolic wastewater place.
2, the yeast extract protein culture medium of bacterium source with the phenolic wastewater preparation screened, filter out well-grown bacterial classification; Screening method is: medium salts content is transferred to 2-25%, inoculum size with 10% inserts the bacterium source in substratum then, pick out the bacterium source of 4 kinds of well-grown bacterial classifications as the efficient Phenol-Containing Wastewater Treatment of screening, the bacterial classification that filters out carries out stored refrigerated respectively under 4 ℃.
The yeast extract protein culture medium consists of: phenol content is 100mg/L in the waste water, salts contg 2-25wt%, contain yeast extract 10.0g in every liter of waste water, peptone 7.5g, Trisodium Citrate 3.0g, Repone K 2.0g, sal epsom 20.0g, to 8.5-9.5, and pass through high pressure steam sterilization with 30% liquid adjusting PH with base.
3, cryopreserved halophilic bacterium bacterial classification is inoculated in the yeast extract protein culture medium (nutrient media components is the same) by 10% inoculum size, place constant temperature shaking table shaking culture, culture condition: temperature is 32
oC, rotating speed are under the rotating speed of 120rpm, and continuous oscillation was cultivated 72 hours.
4, with the substratum centrifugation after each constant temperature shaking table shaking culture, get the DIFFERENT WET thalline, wet thallus joins respectively in the 250mL triangular flask that the 100mL inorganic medium is housed by the amount of 1g/100mL waste water, place the constant temperature culture shaking table, 32 ℃, the 120rpm rotating speed is cultivated 48h down, the removal effect of COD and aldehydes matter in the detection substratum, screening COD degradation rate is more than 50%, the aldehydes matter clearance carries out stored refrigerated respectively at the halophilic bacterium bacterial classification of the efficient degradation aldehydes matter more than 90% under 4 ℃.Adopt present method screening to obtain the halophilic bacterium bacterial classification of 4 kinds of efficient degradation aldehydes matters.
Used inorganic medium is 2, the inorganic medium of 4-D phenolic wastewater preparation, component is: potassium primary phosphate 0.23-0.26g/L, dipotassium hydrogen phosphate concentration is 0.23-0.26g/L, sal epsom 1.9-2.1g/L, calcium chloride 0.18-0.21g/L, iron(ic) chloride 0.09-0.11g/L, ammonium chloride 0.9-1.1g/L, containing phenol concentration in the waste water is that 0.1-2.5g/L, sodium chloride content are 2-25%.
5, the halophilic bacterium bacterial classification to 4 kinds of efficient degradation aldehydes matters filtering out dilutes coating respectively, isolates single bacterium, and each single bacterium is preserved down at 4 ℃.
Two, isolated 4 kinds of efficient phenol-degrading halophilic bacteria list bacterium are tamed respectively, determined the tolerance of bacterial classification phenol
(a) with 10% inoculum size a certain efficient phenol-degrading halophilic bacteria list bacterium is inoculated at first that to contain phenol be 2 of 100mg/L, in the 4-D phenolic wastewater, at 32 ℃, the 120rpm rotating speed was cultivated 72 hours down;
(b) more above-mentioned bacterial classification is inoculated into by 10% inoculum size that to contain phenol be 2 of 300mg/L, in the 4-D phenolic wastewater, at 32 ℃, the 120rpm rotating speed was cultivated 72 hours down;
(c) constantly improve the concentration of aldehydes matter according to above method, finally obtain the halophilic bacterium bacterial classification that the phenolic wastewater of aldehydes matter content below 2500mg/l can both be degraded by continuous domestication.
The acclimation method of other bacterial classifications is identical, and the strain domestication process can be referring to Fig. 1.With after the above-mentioned instructionization can the phenolic wastewater of degrading phenol substances content below 2500mg/L 4 kinds of bacterial classifications dilute coating respectively, isolate single bacterium, and with the preservation under 4 ℃ of each single bacterium, these four kinds of single bacterium are CGMCCNO.4327, CGMCCNO.4328, CGMCCNO.4329, the CGMCCNO.4330 of the present invention's preservation.
Embodiment 1
The bacterial classification that the present invention's screening is obtained mixes by proportioning, the required halophilic bacterium microbial inoculum of phenolic wastewater that obtains degrading, and microbial inoculum consists of: 7 parts of CGMCCNO.4327,4 parts of CGMCCNO.4328,9 parts of CGMCCNO.4329,8 parts of CGMCCNO.4330.
The preparation method of this halophilic bacterium microbial inoculum is: 4 kinds of bacterial classifications are inoculated into respectively in the substratum, and inoculum size is 10wt%, under temperature 15-35 ℃, the condition of pH8.5-9.5, dissolved oxygen 2-4mg/L bacterial classification is carried out enlarged culturing, and incubation time is 72-96h.Used substratum is glucose sugar 9-11g/L, urea 1.0-1.2g/L, potassium primary phosphate 1.29-1.34g/L, sodium-chlor 15wt%.After the cultivation each substratum is carried out centrifugation and get wet thallus, each wet thallus is mixed promptly getting the halophilic bacterium microbial inoculum by said ratio.
The bacterial classification that the present invention's screening is obtained mixes by proportioning, the required halophilic bacterium microbial inoculum of phenolic wastewater that obtains degrading, and microbial inoculum consists of: 4 parts of CGMCCNO.4327,8 parts of CGMCCNO.4328,3 parts of CGMCCNO.4329,10 parts of CGMCCNO.4330.
The preparation method of this halophilic bacterium microbial inoculum is: 4 kinds of bacterial classifications are inoculated into respectively in the substratum, and inoculum size is 10wt%, under temperature 15-35 ℃, the condition of pH 8.5-9.5, dissolved oxygen 2-4mg/L the halophilic bacterium bacterial classification is carried out enlarged culturing 72-96h.Used substratum is glucose sugar 9-11g/L, urea 1.0-1.2g/L, potassium primary phosphate 1.29-1.34g/L, sodium-chlor 4wt%.After the cultivation each substratum is carried out centrifugation and get wet thallus, each wet thallus is mixed promptly getting the halophilic bacterium microbial inoculum by said ratio.
The bacterial classification that the present invention's screening is obtained mixes by proportioning, the required halophilic bacterium microbial inoculum of phenolic wastewater that obtains degrading, and microbial inoculum consists of: 10 parts of CGMCCNO.4327,10 parts of CGMCCNO.4328,5 parts of CGMCCNO.4329,4 parts of CGMCCNO.4330.
The preparation method of this halophilic bacterium microbial inoculum is: 4 kinds of bacterial classifications are inoculated into respectively in the substratum, and inoculum size is 10wt%, under temperature 15-35 ℃, the condition of pH8.5-9.5, dissolved oxygen 2-4mg/L the halophilic bacterium bacterial classification is carried out enlarged culturing 72-96h.Used substratum is glucose sugar 9-11g/L, urea 1.0-1.2g/L, potassium primary phosphate 1.29-1.34g/L, sodium-chlor 24wt%.After the cultivation each substratum is carried out centrifugation and get wet thallus, each wet thallus is mixed promptly getting the halophilic bacterium microbial inoculum by said ratio.
Set forth application and the effect of halophilic bacterium microbial inoculum of the present invention in Phenol-Containing Wastewater Treatment below by specific embodiment
Embodiment 4
Before handling waste water, the halophilic bacterium microbial inoculum is fixed in the biological treatment system, this biological treatment system comprises one-level anaerobic biofilter, secondary anaerobic biological filter, one-level aerobic biofilter and the two stage biological filter tank that connects successively, and the halophilic bacterium microbial inoculum is fixed in one-level anaerobic biofilter, anaerobic biofilter, one-level aerobic biofilter and the two stage biological filter tank.Fixation procedure is: the dosage of halophilic bacterium microbial inoculum by 1-5g/L waste water joined in the bio-reactor that has carrier, microbial inoculum is fixed on the carrier, the set time is 24-48h.
Fixedly the carrier of microbial inoculum can be selected from any in haydite, volcanized rock, urethane floating stuffing, granulated active carbon and the high temperature bamboo charcoal, preferably and commonly used be granulated active carbon.The used urethane floating stuffing of the present invention is from the Bi Wofeng of Foshan City bio tech ltd, and used high temperature bamboo charcoal is from the positive gac in sky, Xuzhou factory.Carrier can choose at random when handling waste water.
Embodiment 5
With 2, the phenolic wastewater of 4-D production plant is an example below, adopts the biological treatment system of the halophilic bacterium microbial inoculum that is fixed with embodiment 1 that the high phenol waste water of high salt is handled, phenol content is below near the 2500mg/L in the waste water, COD is below 15000mg/L, and salt concn is between 2-26%, and contained phenol is phenol, 2 in the waste water, 4-D class phenol, nitrophenols, 2,4, at least a in 6-trichlorophenol and adjacency pair two chlorophenols got 19 group 2 respectively, the 4-D phenolic wastewater, salt, phenol content see the following form 1 in the waste water:
Above-mentioned 19 groups of phenolic wastewater treatment steps are as follows:
(1) the saliferous phenolic wastewater is entered preparing pool, regulate COD, N content and P content in the waste water, make COD:N concentration: P concentration is 200:5:1, if the ratio between this three does not satisfy 200:5:1, can add urea and regulate N content, adds KH
2PO
4Regulate P content, make to satisfy condition, if salts contg then will carry out corresponding adjusting and make the waste water saltiness in the 2-25wt% scope outside 2-25wt% in the waste water;
(2) waste water deployed after, enter the biological treatment system biodegrade, in the biological treatment system, firsts and seconds anaerobic biofilter wastewater treatment condition is: pH 8.5-9.5, temperature 15-35 ℃, time 20-24h; One-level aerobic biofilter wastewater treatment condition is: pH 8.5-9.5, temperature 15-35 ℃, time 20-24h, dissolved oxygen 2-4mg/L; Wastewater treatment condition in two stage biological filter tank is: pH 8.5-9.5, temperature 15-35 ℃, time 20-24h, dissolved oxygen 2-4mg/L, COD ﹤ 200mg/L in the processed waste water, phenol ﹤ 0.2mg/L;
(3) after waste water is carried out a biological disposal upon, further adopt magnetism macroporous ion exchange resin to adsorb, wastewater pH is 6-9 during absorption, the waste water flow velocity is between 15-30mL/min, adsorption temp is 25-35 ℃, TOC ﹤ 20mg/L in the waste water of absorption back, and this detection of phenolic group does not go out;
(4) waste water after the absorption enter the chlor-alkali process for refining by chlor-alkali once refining and secondary refining, can satisfy chlor-alkali starting material needs; Waste water after the absorption also can reclaim solid salt by after the airing of saltern, and resource is fully reclaimed, and has reduced the environmental pollution and the wasting of resources.
In the wastewater treatment process, the disposition of phenol, COD such as following table 2 in the waste water:
Embodiment 6
Be example below with the phenol resin production waste water, employing is fixed with the biological treatment system of the halophilic bacterium microbial inoculum of embodiment 2 the high phenol waste water of high salt is handled, phenol content is 2500mg/L in the waste water, COD is 15000mg/L, contained phenol is mainly phenol in the waste water, and regulating the waste water salt concn is 18%, guarantees that the microbial inoculum dosage is 5g/L, treating processes is with embodiment 5, its result such as following table 3:
Embodiment 7
Be example with the coke-oven gas factory effluent below, employing is fixed with the biological treatment system of the halophilic bacterium microbial inoculum of embodiment 3 the high phenol waste water of high salt is handled, phenol content is 2500mg/L in the waste water, COD is 15000mg/L, contained phenol is mainly phenol in the waste water, and regulating the waste water salt concn is 12%, guarantees that the microbial inoculum dosage is 5g/L, treating processes is with embodiment 5, its result such as following table 4:
Claims (10)
1. a halophilic bacterium microbial inoculum is characterized in that: comprise Providence (Providencia sp.) CJ-4 CGMCC NO.4327, Oceanobacillus sp.CJ-10 CGMCC NO.4328, brevibacterium epidermidis (Brevibacterium epidermidis) CJ-12 CGMCC NO.4329 and salt Zymomonas mobilis (Halomonas sp.) CJ-13 CGMCC NO.4330.
2. halophilic bacterium microbial inoculum according to claim 1 is characterized in that: the weight proportion of each component is in the microbial inoculum:
Providence (Providencia sp.) CJ-4 CGMCC NO.4327 4-10 part;
Oceanobacillus sp.CJ-10 CGMCC NO.4328 4-10 part;
Brevibacterium epidermidis (Brevibacterium epidermidis) CJ-12 CGMCC NO.4329 3-9 part;
Salt Zymomonas mobilis (Halomonas sp.) CJ-13 CGMCC NO.4330 4-10 part.
3. the preparation method of the described halophilic bacterium microbial inoculum of claim 1, it is characterized in that: each bacterial classification is linked in the substratum by the inoculum size of 10wt% respectively, under temperature 15-35 ℃, the condition of pH 8.5-9.5, dissolved oxygen 2-4mg/L, bacterial classification is carried out enlarged culturing 72-96h, after the cultivation each substratum centrifugation is got wet thallus, the gained wet thallus is mixed by proportioning promptly get halophilic bacterium microbial inoculum of the present invention; Used substratum is glucose sugar 9-11g/L, urea 1.0-1.2g/L, potassium primary phosphate 1.29-1.34g/L, sodium-chlor 2-25wt%.
4. biological treatment system, it is characterized in that comprising: the one-level anaerobic biofilter of Lian Jieing, secondary anaerobic biological filter, one-level aerobic biofilter and secondary aerobic biofilter successively, all be fixed with the described halophilic bacterium microbial inoculum of claim 1 in described one-level anaerobic biofilter, secondary anaerobic biological filter, one-level aerobic biofilter and the two stage biological filter tank, the fixed amount of halophilic bacterium microbial inoculum is 1-5g/L waste water.
5. biological treatment system according to claim 4 is characterized in that: fixedly the used carrier of halophilic bacterium is haydite, volcanized rock, urethane floating stuffing, granulated active carbon or high temperature bamboo charcoal, and the set time is 24-48h.
6. according to claim 4 or 5 described biological treatment systems, it is characterized in that: fixedly the used carrier of halophilic bacterium is a granulated active carbon.
7. high phenol wastewater treatment method of high salt is characterized in that may further comprise the steps at least:
(1) the saliferous phenolic wastewater enters preparing pool, and COD, N in the adjusting waste water and the content of P are adjusted to 2-25wt% with salts contg in the waste water simultaneously;
(2) waste water enters claim 4 or 5 described biological treatment system biodegrades, COD ﹤ 200mg/L in the processed waste water, phenol ﹤ 0.2mg/L;
(3) biological treatment back waste water further adopts magnetism macroporous ion exchange resin to adsorb, TOC ﹤ 20mg/L in the waste water of absorption back;
(4) waste water after the step (3) reclaims solid salt after entering the chlor-alkali process for refining or entering the saltern airing.
8. treatment process according to claim 7 is characterized in that: in the biological treatment system, firsts and seconds anaerobic biofilter wastewater treatment condition is: pH 8.5-9.5, temperature 15-35 ℃, time 20-24h; One-level aerobic biofilter wastewater treatment condition is: pH 8.5-9.5, temperature 15-35 ℃, time 20-24h, dissolved oxygen 2-4mg/L; Wastewater treatment condition in two stage biological filter tank is: pH 8.5-9.5, temperature 15-35 ℃, time 20-24h, dissolved oxygen 2-4mg/L.
9. treatment process according to claim 7 is characterized in that: in the step (1), add urea and regulate N content in the waste water, add KH
2PO
4Regulate P content in the waste water, the concentration ratio that makes COD:N:P in the waste water is 200:5:1; In the step (3), biological treatment back wastewater pH 6-9, flow velocity is 15-30mL/min, adsorption temp is 25-35 ℃.
10. treatment process according to claim 7 is characterized in that: phenol content is below 2500mg/L in the waste water, and COD is below 15000mg/L, contained phenol is phenol, 2 in the waste water, 4-D class phenol, nitrophenols, 2,4, at least a in 6-trichlorophenol and adjacency pair two chlorophenols.
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