CN112175934A - Microbial material with salt-tolerant denitrification capability and preparation method and application thereof - Google Patents

Microbial material with salt-tolerant denitrification capability and preparation method and application thereof Download PDF

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CN112175934A
CN112175934A CN202011077561.3A CN202011077561A CN112175934A CN 112175934 A CN112175934 A CN 112175934A CN 202011077561 A CN202011077561 A CN 202011077561A CN 112175934 A CN112175934 A CN 112175934A
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祝惠
王鑫壹
阎百兴
程锐
曹书境
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Northeast Institute of Geography and Agroecology of CAS
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Abstract

A microbial material with salt-tolerant denitrification capability and a preparation method and application thereof relate to the technical field of environmental microorganisms, in particular to a microbial material and a preparation method and application thereof. The method aims to solve the problem that the existing method for directly adding the salt-tolerant denitrification microbial inoculum has short denitrification effect maintaining time. The microbial material is prepared from bacterial suspension, an external carbon source and an embedding medium. The method comprises the following steps: the method comprises the following steps: modifying an external carbon source; step two: preparing a bacterial suspension; step three: and (4) embedding preparation of the microbial material. The microbial material has high-efficiency salt-tolerant denitrification capability, and can efficiently remove nitrogen pollutants in wastewater under the condition of salt stress. In addition, the microbial material contains rich additional carbon sources, can make up for the problem of insufficient carbon sources, provides nutrients required for growth of microorganisms in a salt stress environment, and promotes the growth and metabolism of denitrifying bacteria in a system. The invention is applied to the denitrification treatment of the saline water body.

Description

Microbial material with salt-tolerant denitrification capability and preparation method and application thereof
Technical Field
The invention relates to the technical field of environmental microorganisms, in particular to a microbial material and a preparation method and application thereof.
Background
In recent years, with the continuous increase of industrial scale in China, the industrial water consumption is increased dramatically. Meanwhile, the amount of generated wastewater is rapidly increased, and great challenges are brought to the current wastewater treatment and recycling technology. Among discharged wastewater, saline wastewater is becoming a non-negligible class, and such wastewater is receiving increasing attention. Among them, the salt-containing sewage with high nitrogen content often causes eutrophication of water, affects the normal survival of animals and plants therein, and threatens ecological balance. Eutrophication is a major problem of water pollution in many countries. Therefore, the treatment of the saline sewage, especially the denitrification of the saline sewage, is a problem to be solved.
The artificial wetland is a water treatment technology with low cost, high efficiency, energy conservation and no secondary pollution, and is widely applied to the aspect of purifying water bodies. However, the salt-containing wastewater not only has complex pollutant components, but also contains a large amount of soluble inorganic salt, and the growth and metabolism of microorganisms in the artificial wetland can be inhibited by high salinity, so that the removal capability of the artificial wetland on the pollutants in the wastewater is limited. Under the salt stress, some inherent microorganisms with certain salt tolerance in the wetland can survive and are domesticated gradually to become dominant flora in the artificial wetland, and other microorganisms with lower salt tolerance are eliminated gradually. The salt-tolerant microorganisms have a complex salt-tolerant mechanism in a salt stress environment, can normally grow and metabolize in a large-range salinity environment, and have high-efficiency degradation capability on various pollutants. Although the inherent salt-tolerant microorganisms are domesticated in the artificial wetland, the contribution of the salt-tolerant microorganisms to the aspect of purifying the water body is low. Namely, the denitrification efficiency of the inherent microorganisms in the wetland is low, and the requirement of purifying the water body in the artificial wetland cannot be met.
The purification function of the artificial wetland under salt stress can be improved by utilizing an exogenous microorganism strengthening technology. The direct addition of the salt-tolerant denitrification microbial inoculum can only maintain the short-term strengthening effect, and the exogenous microorganisms do not have attachment points in the artificial wetland and are easy to lose along with wastewater.
Disclosure of Invention
The invention provides a microbial material with salt-tolerant denitrification capability and a preparation method thereof, aiming at solving the problem of short denitrification effect maintaining time in the existing method of directly adding salt-tolerant denitrification microbial inoculum.
The microbial material with salt-tolerant denitrification capability is prepared from bacterial suspension, an external carbon source and an embedding agent. Wherein the strain in the bacterial suspension is Shewanella allowachii Alishewanella sp.F2.
Further, the Shewanella allowaensis sp.F2(GenBank accession No. MN396708) is deposited in the China general microbiological culture Collection center, the preservation date is 3 and 25 days in 2019, and the preservation number is CGMCC No: 17433.
further, the external carbon source is corncob meal modified by NaOH solution.
Further, the embedding medium is composed of polyvinyl alcohol and alginic acid, and the mass ratio of the polyvinyl alcohol to the alginic acid is 5: 1.
The invention also provides a preparation method of the microbial material with salt-tolerant denitrification capability, which comprises the following steps:
the method comprises the following steps: modification of an external carbon source
Selecting corncob meal as an external carbon source, dissolving the corncob meal in NaOH solution, and heating in a water bath kettle at the temperature of 90-95 ℃, wherein the heating is carried out while continuously stirring; after the heating treatment, washing and soaking with distilled water sequentially for three times, adjusting the pH to be neutral in the third soaking process, and then cleaning once again; finally, drying to obtain modified corncob powder;
step two: preparation of the bacterial suspension
Enrichment culture is carried out on Shewanella allowachii Alishhewanella sp.F2, and the culture medium is used as enriched seed bacterial liquid after turbidity and gas generation appear; inoculating the enriched seed bacterial liquid into a denitrification culture medium with an inoculation amount of 5% (v/v), and sealing and standing at a constant temperature of 30-32 ℃ for 5-7 d; finally, centrifuging the culture solution to obtain concentrated somatic cells, and diluting and uniformly mixing the somatic cells with sterile water to obtain a bacterial suspension;
step three: embedding preparation of microbial material
Adding an embedding agent and modified corncob powder into the bacterial suspension obtained in the step two, uniformly mixing, continuously stirring, and adjusting the pH to 10 to obtain a mixed solution; then the mixed solution was added dropwise to CaCl2And (3) forming gel particles in the solution, standing the gel particles at room temperature for 6-8 h, and washing with sterile water for 2-3 times to obtain the microbial material.
Furthermore, in the first step, the mass ratio of the corncob powder to the NaOH solution is 1 (29-31). The mass concentration of the NaOH solution is 1%.
Further, the time of the heating treatment in the step one is 1-1.2 h.
Further, the drying temperature in the first step is 70-75 ℃.
Further, the modified corncob meal obtained in the step one is placed in a drying dish at room temperature for storage.
Further, the enrichment culture in the second step is performed under the condition of constant temperature sealing and static culture at 30-32 ℃.
Further, the denitrification culture medium in the second step comprises the following components: CH (CH)3COONa，5g/L；K2HPO4，1g/L； NaNO2，0.8g/L；CaCl2，0.03g/L；Na2CO3，1g/L；FeSO4·7H2O，0.06g/L；MgSO4·7H2O，0.2g/L；pH＝10。
Further, the rotating speed of centrifugation in the second step is 4000-6000 rpm, and the centrifugation time is 15-20 min.
Further, the concentration of the bacterial suspension in the second step is 1.5-2 g of concentrated bacterial cells/L, namely 1.5-2 g of concentrated bacterial cells obtained by centrifugation are contained in each liter of bacterial suspension.
Furthermore, the mass ratio of the embedding agent to the modified corncob powder in the third step is 3: 1.
Furthermore, the ratio of the total mass of the embedding agent and the modified corncob meal to the volume of the bacterial suspension in the third step is 160g to 1L.
Further, the specific method of dripping in the third step is to use a syringe needle to drip the mixed solution into CaCl with the mass concentration of 4 percent2In the solution, the solution was shaken while dropping.
The application of the microbial material with salt-tolerant denitrification capability in denitrification of the salt-containing water body.
Further, the adding amount of the microbial material in the saline water body is 50 g/L.
Further, the salt-containing condition of the salt-containing water body is that EC is 15 mS/cm.
The invention has the beneficial effects that:
the invention provides a microbial material (bacterium granule) which is prepared by jointly immobilizing an external carbon source and a target bacterium, can improve the environmental adaptability of the target bacterium and avoid the loss of the bacterium. The invention provides the practical application of the prepared microbial material in the artificial wetland, and finds that the microbial material can effectively improve the removal capability of the artificial wetland on nitrogen pollutants under the salt stress. The microbial material with the salt-tolerant denitrification capability has the physiological and biochemical characteristics and metabolic mechanism of efficient salt-tolerant denitrification bacteria, can provide an external carbon source for the growth and metabolism of microorganisms in the artificial wetland, and can better solve the problem of the artificial wetland in the treatment of salt-containing wastewater.
The microbial material has high-efficiency salt-tolerant denitrification capability, and can efficiently remove nitrogen pollutants in wastewater under the condition of salt stress. In addition, the microbial material contains rich additional carbon sources, can make up for the problem of insufficient carbon sources, provides nutrients required for growth of microorganisms in a salt stress environment, and promotes the growth and metabolism of denitrifying bacteria in a system. The microbial material has the advantages of low manufacturing cost, simple operation and good denitrification effect.
The microbial material plays an important role in salt-containing wastewater (EC is 15mS/cm, and the concentration of NaCl is about 8g/L), and can efficiently remove nitrogen pollutants in the wastewater. Wherein, under the inoculation amount of 5 percent (m/v), the removal rates of ammonia nitrogen, nitrate nitrogen and total nitrogen in the salt-containing wastewater are 74.61 percent, 97.65 percent and 47.63 percent respectively.
The microbial material disclosed by the invention can improve the denitrification capability of the constructed wetland under the salt stress. Specifically, 30 days after the microbial material is added, the removal rate of total nitrogen in the salt-containing wastewater by the artificial wetland is over 90 percent, which is obviously higher than that of a treatment group (about 80 percent) in which a microbial inoculum is directly added at the same period.
Drawings
FIG. 1 shows the immobilized form of the microbial material according to the present invention;
FIG. 2 shows the ammonia nitrogen concentration measured at different times;
FIG. 3 is a graph showing the nitrate nitrogen concentration measured at different times;
FIG. 4 is a graph of total nitrogen concentration measured at various times.
Detailed Description
The technical solution of the present invention is not limited to the following specific embodiments, but includes any combination of the specific embodiments.
The first embodiment is as follows: the microbial material with salt-tolerant denitrification capability is prepared from bacterial suspension, an external carbon source and an embedding agent. Wherein the strain in the bacterial suspension is Shewanella allowachii Alishewanella sp.F2.
F2(GenBank access No. MN396708) is preserved in the China general microbiological culture Collection center of China general microbiological culture Collection management Committee, the preservation date is 3-25 months in 2019, and the preservation number is CGMCC No: 17433.
the second embodiment is as follows: the first difference between the present embodiment and the specific embodiment is: the external carbon source is corncob powder modified by NaOH solution. The rest is the same as the first embodiment.
The third concrete implementation mode: the first difference between the present embodiment and the specific embodiment is: the embedding medium is composed of polyvinyl alcohol and alginic acid, and the mass ratio of the polyvinyl alcohol to the alginic acid is 5: 1. The rest is the same as the first embodiment.
The fourth concrete implementation mode: the preparation method of the microbial material with salt and nitrogen resistance comprises the following steps:
the method comprises the following steps: modification of an external carbon source
Selecting corncob meal as an external carbon source, dissolving the corncob meal in NaOH solution, and heating in a water bath kettle at the temperature of 90-95 ℃, wherein the heating is carried out while continuously stirring; after the heating treatment, washing and soaking with distilled water sequentially for three times, adjusting the pH to be neutral in the third soaking process, and then cleaning once again; finally, drying to obtain modified corncob powder;
step two: preparation of the bacterial suspension
Enrichment culture is carried out on Shewanella allowachii Alishhewanella sp.F2, and the culture medium is used as enriched seed bacterial liquid after turbidity and gas generation appear; inoculating the enriched seed bacterial liquid into a denitrification culture medium with an inoculation amount of 5% (v/v), and sealing and standing at a constant temperature of 30-32 ℃ for 5-7 d; finally, centrifuging the culture solution to obtain concentrated somatic cells, and diluting and uniformly mixing the somatic cells with sterile water to obtain a bacterial suspension;
step three: embedding preparation of microbial material
Adding an embedding agent and modified corncob powder into the bacterial suspension obtained in the step two, uniformly mixing, continuously stirring, and adjusting the pH to 10 to obtain a mixed solution; then the mixed solution was added dropwise to CaCl2And (3) forming gel particles in the solution, standing the gel particles at room temperature for 6-8 h, and washing with sterile water for 2-3 times to obtain the microbial material.
The fifth concrete implementation mode: the fourth difference between this embodiment and the specific embodiment is that: in the first step, the mass ratio of the corncob powder to the NaOH solution is 1 (29-31). The mass concentration of the NaOH solution is 1%. The rest is the same as the fourth embodiment.
The sixth specific implementation mode: the fourth difference between this embodiment and the specific embodiment is that: the time of the heating treatment in the step one is 1-1.2 h. The rest is the same as the fourth embodiment.
The seventh embodiment: the fourth difference between this embodiment and the specific embodiment is that: the drying temperature in the first step is 70-75 ℃. The rest is the same as the fourth embodiment.
The specific implementation mode is eight: the fourth difference between this embodiment and the specific embodiment is that: and (4) placing the modified corncob powder obtained in the step one in a drying dish at room temperature for storage. The rest is the same as the fourth embodiment.
The specific implementation method nine: the fourth difference between this embodiment and the specific embodiment is that: and the enrichment culture in the second step is performed under the condition of constant temperature sealing and static culture at the temperature of 30-32 ℃. The rest is the same as the fourth embodiment.
The detailed implementation mode is ten: the fourth difference between this embodiment and the specific embodiment is that: the denitrification culture medium in the second step comprises the following components: CH (CH)3COONa，5g/L；K2HPO4，1g/L；NaNO2，0.8g/L；CaCl2，0.03g/L；Na2CO3， 1g/L；FeSO4·7H2O，0.06g/L；MgSO4·7H2O, 0.2 g/L; pH 10. The rest is the same as the fourth embodiment.
The concrete implementation mode eleven: the fourth difference between this embodiment and the specific embodiment is that: and in the second step, the rotating speed of the centrifugation is 4000-6000 rpm, and the centrifugation time is 15-20 min. The rest is the same as the fourth embodiment.
The specific implementation mode twelve: the fourth difference between this embodiment and the specific embodiment is that: the concentration of the bacterial suspension in the second step is 1.5-2 g of concentrated somatic cells/L, namely 1.5-2 g of concentrated somatic cells obtained by centrifugation are contained in each liter of bacterial suspension. The rest is the same as the fourth embodiment.
The specific implementation mode is thirteen: the fourth difference between this embodiment and the specific embodiment is that: in the third step, the mass ratio of the embedding agent to the modified corncob powder is 3: 1. The rest is the same as the fourth embodiment.
The specific implementation mode is fourteen: the fourth difference between this embodiment and the specific embodiment is that: in the third step, the ratio of the total mass of the embedding agent and the modified corncob powder to the volume of the bacterial suspension is 160g to 1L. The rest is the same as the fourth embodiment.
The concrete implementation mode is fifteen: the fourth difference between this embodiment and the specific embodiment is that: the specific method of dripping in the third step is that the syringe needle is used for dripping the mixed liquid into CaCl with the mass concentration of 4 percent2In the solution, the solution was shaken while dropping. The rest is the same as the fourth embodiment.
The specific implementation mode is sixteen: the fourth difference between this embodiment and the specific embodiment is that: in the third step, the embedding medium is composed of polyvinyl alcohol and alginic acid according to the mass ratio of 5: 1. The rest is the same as the fourth embodiment.
Seventeenth embodiment: the embodiment provides application of the microbial material with salt-tolerant denitrification capability in denitrification of the salt-containing water body.
The specific implementation mode is eighteen: the present embodiment is different from the seventeenth embodiment in that: the adding amount of the microbial material in the saline water body is 50 g/L. The rest is the same as in the seventeenth embodiment.
The detailed embodiment is nineteen: the present embodiment is different from the seventeenth embodiment in that: the salt-containing condition of the salt-containing water body is EC 15 mS/cm. The rest is the same as in the seventeenth embodiment.
The following examples are given to illustrate the present invention, and the following examples are carried out on the premise of the technical solution of the present invention, and give detailed embodiments and specific procedures, but the scope of the present invention is not limited to the following examples.
Example 1: preparation condition optimization of microbial material
The microbial material adopts bacterial suspension as a solvent, and the concentration of a target strain is a fixed value, namely 1.5-2 g/L. Therefore, when the optimal preparation condition is selected, the COD release amount of the external carbon source is mainly taken as a primary index for measuring the microbial material; meanwhile, physical characteristics of the microbial material are considered, and mainly include molding condition, adhesion condition, elasticity, strength, stability and the like of the immobilized particles. Designing an orthogonal test, and carrying out the test according to an orthogonal table with five factors and four levels, wherein 5 selected factors are polyvinyl alcohol, sodium alginate, modified corncob powder, pH and immobilization time respectively, and the four levels corresponding to the factors are shown in table 1. According to the preparation method, 10g of each particle formed by 16 groups of different factors and horizontal combination immobilization is added into a 250mL conical flask filled with 200mL of distilled water, the bottle mouth is sealed, the mixture is placed at room temperature and stands for immobilization time corresponding to each treatment group, and the COD concentration is measured. According to various research indexes, an optimal preparation condition is obtained by adopting a range difference method, namely 100g/L of polyvinyl alcohol, 20g/L of sodium alginate, 40g/L of modified corncob powder, pH value of 10 and immobilization time of 6 h. The morphological characteristics of the prepared microbial material are shown in figure 1.
TABLE 1 orthogonal experimental design table for determination of optimal immobilization conditions
Figure BDA0002718041160000061
Example 2: denitrifying performance experiment of microbial material
The preparation method of the microbial material with salt and nitrogen tolerance capability comprises the following steps:
the method comprises the following steps: modification of an external carbon source
Selecting corncob meal as an external carbon source, dissolving the corncob meal in NaOH solution, carrying out heating treatment for 1-1.2 h in a water bath kettle at the temperature of 90 ℃, and continuously stirring while carrying out heating treatment; after the heating treatment, washing and soaking with distilled water sequentially for three times, adjusting the pH to be neutral in the third soaking process, and then cleaning once again; finally, drying at 70 ℃ to obtain modified corncob powder; wherein the mass ratio of the corncob powder to the NaOH solution is 1: 30; the mass concentration of the NaOH solution is 1%;
step two: preparation of the bacterial suspension
For different ShewashF2, carrying out enrichment culture on the strain Alishewanella sp.F2, wherein the culture condition is constant-temperature sealed standing culture at 30-32 ℃, and taking the strain as an enriched seed bacterial solution after the culture medium is turbid and generates gas; inoculating the enriched seed bacterial liquid into a denitrification culture medium with an inoculation amount of 5% (v/v), and sealing and standing at a constant temperature of 30-32 ℃ for 5-7 d; finally, centrifuging the culture solution at the centrifugal rotation speed of 5000rpm for 15min to obtain concentrated somatic cells, diluting and uniformly mixing the concentrated somatic cells with sterile water to obtain a bacterial suspension, wherein each liter of the bacterial suspension contains 1.5-2 g of the concentrated somatic cells obtained by centrifugation; wherein the denitrification culture medium comprises the following components: CH (CH)3COONa，5g/L；K2HPO4，1g/L；NaNO2，0.8g/L；CaCl2， 0.03g/L；Na2CO3，1g/L；FeSO4·7H2O，0.06g/L；MgSO4·7H2O，0.2g/L；pH＝10。
Step three: embedding preparation of microbial material
Adding an embedding agent (consisting of polyvinyl alcohol and alginic acid according to the mass ratio of 5: 1) and modified corncob powder into the bacterial suspension obtained in the step two, uniformly mixing, continuously stirring, and adjusting the pH value to 10 to obtain a mixed solution; then the mixed solution was added dropwise to CaCl2The specific method of dripping into the solution is to drip the mixed solution into CaCl with the mass concentration of 4% by using an injector needle2And dripping while shaking in the solution to form gel particles, standing the gel particles at room temperature for 6-8 h, and washing with sterile water for 2-3 times to obtain the microbial material. Wherein the mass ratio of the embedding agent to the modified corncob powder is 3: 1. The volume ratio of the total mass of the embedding agent and the modified corncob powder to the bacterial suspension is 160g to 1L.
0g, 10g, 30g and 50g of the microbial material prepared in the embodiment are respectively weighed and added into a beaker filled with 1000mL of artificial simulated saline wastewater (EC is 15mS/cm, NaCl concentration is about 8g/L, and ammonia nitrogen, nitrate nitrogen, phosphate and COD concentration is about 10mg/L, 5mg/L, 3mg/L and 10mg/L respectively), the beaker is sealed by a preservative film, the beaker is placed at room temperature, water samples are collected at regular time every day, and the concentrations of ammonia nitrogen, nitrate nitrogen and total nitrogen are determined in an emphasis manner. The treatment groups without microbial material added are used as blank control, and each group is provided with 3 groups in parallel. The results are as followsAs shown in FIGS. 2 to 4,
Figure BDA0002718041160000071
represents that the addition amount of the microbial material in the wastewater is 0%,
Figure BDA0002718041160000072
represents that the addition amount of the microbial material in the wastewater is 1%,
Figure BDA0002718041160000073
represents that the addition amount of the microbial material in the wastewater is 3%,
Figure BDA0002718041160000074
indicating that the amount of the microbial material added to the wastewater was 5%. The removal rate of ammonia nitrogen, nitrate nitrogen and total nitrogen in each treatment group is gradually increased along with the increase of time; under the condition that the adding amount of the microbial material is 50g/L, the removal rates of ammonia nitrogen, nitrate nitrogen and total nitrogen are the largest, and at the 5d, the removal rates are 74.61%, 97.65% and 47.63% respectively, which are obviously higher than those of a treatment group without adding the microbial material.
Example 3: practical application of microbial material in constructed wetland
The microbial material prepared in example 2 was sufficiently mixed with gravel having a depth of 3cm from the bottom of the artificial wetland simulator in an amount of 50 g/L. The artificial wetland is fed in a vertical flow mode, the quality of the fed water is artificial simulated saline wastewater (the same as the embodiment 2), water samples are collected at regular time every day, and the concentrations of ammonia nitrogen, nitrate nitrogen and total nitrogen are mainly measured. After microbial materials are added, the denitrification capacity of the artificial wetland simulator under the condition of salt stress is improved, and the removal rates of ammonia nitrogen, nitrate nitrogen and total nitrogen are respectively 93%, 93% and over 90%.
The treatment group directly added with the microbial inoculum is taken as a control group. 30 days after the microbial materials are added, the removal rate of the total nitrogen in the salt-containing wastewater by the artificial wetland is over 90 percent, which is obviously higher than that of a treatment group (about 80 percent) directly adding the microbial inoculum at the same time.

Claims (10)

1. A microbial material with salt-tolerant denitrification capability is characterized in that the microbial material is prepared from a bacterial suspension, an external carbon source and an embedding agent; wherein the strain in the bacterial suspension is Shewanella allowachii Alishewanella sp.F2.
2. The microbial material with salt-tolerant denitrification capability of claim 1, wherein the external carbon source is corncob meal modified by NaOH solution.
3. The microbial material with salt-tolerant denitrification capability as claimed in claim 1 or 2, wherein the embedding agent is composed of polyvinyl alcohol and alginic acid, and the mass ratio of the polyvinyl alcohol to the alginic acid is 5: 1.
4. The method for preparing the microbial material with the salt-tolerant denitrification capability of claim 1, wherein the method comprises the following steps:
the method comprises the following steps: selecting corncob meal as an external carbon source, dissolving the corncob meal in NaOH solution, and heating in a water bath kettle at the temperature of 90-95 ℃, wherein the heating is carried out while continuously stirring; after the heating treatment, washing and soaking with distilled water sequentially for three times, adjusting the pH to be neutral in the third soaking process, and then cleaning once again; finally, drying to obtain modified corncob powder;
step two: enrichment culture is carried out on Shewanella allowachii Alishhewanella sp.F2, and the culture medium is used as enriched seed bacterial liquid after turbidity and gas generation appear; inoculating the enriched seed bacterial liquid into a denitrification culture medium with an inoculation amount of 5% (v/v), and sealing and standing at a constant temperature of 30-32 ℃ for 5-7 d; finally, centrifuging the culture solution to obtain concentrated somatic cells, and diluting and uniformly mixing the somatic cells with sterile water to obtain a bacterial suspension;
step three: adding embedding agent and modified corncob powder into the bacterial suspension obtained in the step two, uniformly mixing, continuously stirring, and adjustingAdjusting the pH value to 10 to obtain a mixed solution; then the mixed solution was added dropwise to CaCl2And (3) forming gel particles in the solution, standing the gel particles at room temperature for 6-8 h, and washing with sterile water for 2-3 times to obtain the microbial material.
5. The method for preparing a microbial material with salt-tolerant denitrification capability according to claim 4, wherein the heating treatment time in the first step is 1-1.2 h.
6. The method for preparing a microbial material with salt-tolerant denitrification capability according to claim 4, wherein 1.5-2 g of concentrated bacterial cells obtained by centrifugation is contained in each liter of bacterial suspension in the step two.
7. The method for preparing the microbial material with the salt-tolerant denitrification capability according to claim 4, wherein the mass ratio of the embedding agent to the modified corncob meal in the third step is 3: 1.
8. The method for preparing the microbial material with the salt and nitrogen removal capacity of claim 4, wherein the volume ratio of the total mass of the embedding agent and the modified corncob meal to the bacterial suspension in the third step is 160g: 1L.
9. The use of the microbial material with salt-tolerant denitrification capability of claim 1 in denitrification of saline water.
10. The use according to claim 9, characterized in that the microbial material is dosed at 50g/L in the saline water body.
CN202011077561.3A 2020-10-10 2020-10-10 Microbial material with salt-tolerant denitrification capability and preparation method and application thereof Pending CN112175934A (en)

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Application publication date: 20210105