CN114908002A - Biological nano-selenium reinforced composite strain and application thereof - Google Patents
Biological nano-selenium reinforced composite strain and application thereof Download PDFInfo
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- CN114908002A CN114908002A CN202210391937.0A CN202210391937A CN114908002A CN 114908002 A CN114908002 A CN 114908002A CN 202210391937 A CN202210391937 A CN 202210391937A CN 114908002 A CN114908002 A CN 114908002A
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
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- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F2101/00—Nature of the contaminant
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- C02F2101/163—Nitrates
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Abstract
The invention discloses a biological nano-selenium reinforced composite strain and application thereof. The processing method comprises the following steps: 1) culturing bacillus megaterium to synthesize and prepare biological nano selenium; 2) culturing and domesticating mixed thalli of sphingomonas YT methyl orange, pseudomonas putida, bacilli and gordonia; 3) centrifuging and mixing the thalli provided in the step 1) and the step 2), and inoculating the thalli into a water body to be treated for denitrification treatment. The mixed bacterial strain loaded with biological nano-selenium can efficiently remove ammonia nitrogen and nitrate nitrogen in water, can grow under the condition that the ammonia nitrogen or the nitrate nitrogen is used as a unique nitrogen source, and can perform nitrification-denitrification action under the heterotrophic aerobic condition, so that the nano-selenium/composite heterotrophic nitrification-aerobic denitrification bacteria have great application potential in the aspects of water heterotrophic nitrification-aerobic denitrification.
Description
Technical Field
The invention belongs to the field of environmental microorganisms, and particularly relates to a biological nano-selenium reinforced composite strain and application thereof.
Background
In recent years, a large amount of nitrogen-containing wastewater is discharged into a water body, and the water body eutrophication problem is caused by the over-standard nitrogen element in the water body, so that the water quality is deteriorated. The removal of nitrogen in water becomes an important environmental problem which needs to be solved at present. The biological method has the advantages of simple operation, good treatment effect, no secondary pollution and the like, and is widely used for removing the nitrogen-containing wastewater.
The traditional biological denitrification generally comprises two processes of aerobic nitrification and anaerobic denitrification, and because the two reactions have different requirements on factors such as dissolved oxygen, organic matters and the like, the two reactions are usually independently carried out in two reaction tanks, so that the difficulty and the cost of sewage treatment are obviously increased, and the propagation of microorganisms is slow. Heterotrophic nitrification-aerobic denitrification is used as a novel denitrification technology, nitrification and denitrification can simultaneously occur under heterotrophic and aerobic conditions, the problem of incompatibility of nitrification and denitrification caused by different oxygen demands can be solved, and the capital cost and the operation cost are greatly saved. Meanwhile, the growth rate of the bacteria is high, and the carbon source in the water can be used for the growth of the bacteria and can also be used as an energy source for heterotrophic nitrification and aerobic denitrification. In general A 2 Heterotrophic nitrification-aerobic denitrification bacteria screened from the/O pool mainly aim at conventional municipal wastewater, mostly have better denitrification effect, and can efficiently remove ammonia nitrogen and total nitrogen in the wastewater. However, compared with municipal wastewater, the fermentation pharmaceutical wastewater has the characteristics of high ammonia nitrogen and organic nitrogen concentration, namely BOD 5 the/N is generally between 1 and 4 and is lower than the requirement of conventional biological treatment. In addition, the pharmaceutical wastewater contains a large amount of antibiotics, and the residual antibiotics have strong inhibition and toxicity on microbial activity, so that the biological denitrification effect is limited.
If microorganism or some matrix with specific function is added into the traditional biological treatment system to enhance the degradation capability of the traditional biological treatment system to specific pollutants, thereby improving the treatment effect of the whole sewage treatment system, the technology is called as a biological strengthening technology. In recent years, the reaction rate of the nano material is improved due to the huge specific surface area and high activity of the nano material, and the nano material is applied to remediation of polluted soil and underground water and sewage treatment. Selenium is one of essential trace elements for human and animals, and has important relationship with body oxidation resistance, immunity, antivirus, anticancer effects, etc. Compared with inorganic selenium and organic selenium, the red nano-selenium has the characteristics of low toxicity, high biological activity and the like, and has wide application in animal production, medicine and health care products. At present, the synthesis of nano-selenium is mainly generated by chemical reaction, crystallization technology, reverse micelle method and other technologies, the technologies are limited by conditions of high temperature, high pressure, catalyst and the like, and are easy to cause harm to the environment, the produced nano-selenium is easy to agglomerate without a protective agent, the physicochemical property is unstable, the biological activity is easy to lose at normal temperature, and the nano-selenium is converted into grey black crystal form simple substance selenium. However, the product in the growth and development process of the microorganism reacts with the selenium salt to prepare the nano-selenium, the method has mild reaction conditions, and the prepared nano-selenium has high bioactivity, small size, uniform particle size, no cytotoxicity, strong stability and environmental friendliness. Therefore, the biological synthesis of the nano selenium is combined with the screening of the heterotrophic nitrification-aerobic denitrification bacteria which are tolerant to antibiotics from the fermented pharmaceutical wastewater, and the application of the heterotrophic nitrification-aerobic denitrification bacteria in biological denitrification is of great significance.
Disclosure of Invention
The invention aims to provide a nano selenium reinforced composite strain with high heterotrophic nitrification-aerobic denitrification efficiency, small microbial toxicity and low raw material source price and a preparation method thereof aiming at the defects of the prior art, and the nano selenium reinforced composite strain can be widely applied to the treatment of nitrogen-containing wastewater.
The invention utilizes biological means, utilizes thalli to directly synthesize biological nano-selenium, couples other various strains with aerobic nitrification-heterotrophic denitrification to form synergistic effect on the treatment of the nitrogenous wastewater, not only can utilize the high specific surface area and surface activity of nano-selenium particles, but also can ensure the stability and activity of microorganisms; the prepared biological nano-selenium is suitable for in-situ remediation and has no secondary pollution.
The purpose of the invention is realized by the following technical scheme:
a biological nano-selenium reinforced composite bacterial strain comprises various combinations of bacillus megaterium, sphingomonas YT methyl orange, pseudomonas putida, bacilli and Gordonia for synthesizing biological nano-selenium.
Further, the volume ratio of bacillus megaterium, bacillus, gordonia, pseudomonas putida and sphingomonas YT methyl orange is (10-15%): (15-20%): (10-15%): (10-25%): (30-45%) and the viable count of each strain is greater than 4.5 hundred million/g.
The biological nano selenium reinforced composite strain is applied to total nitrogen treatment of a water body, and the treatment method comprises the following steps:
1) adding Bacillus megaterium solution into 100mL of nutrient solution, and adding 2.2mL of Na containing 1g/L 2 SeO 3 The inoculation amount of the mother liquor is 5-10% of the volume percentage of the nutrient solution, the culture temperature is 30-35 ℃, the rotation speed is 120-150rpm, the culture is carried out for 24-48h, and the centrifugal treatment is carried out to obtain cells in the logarithmic growth phase of the thalli; washing with phosphate buffer solution for 1-2 times to obtain thallus A for synthesizing biological nano-selenium;
2) transferring mixed thallus of pseudomonas putida, bacilliform bacteria, gordonia and sphingomonas YT methyl orange into 100mL of nutrient solution respectively, culturing for 1-3 days at the temperature of 30-35 ℃ and the rotating speed of 150rpm, inoculating the mixed thallus into a container containing a proliferation culture medium according to the volume proportion of 5-18%, culturing for 1-3 days at the temperature of 35-37 ℃, and centrifuging to obtain cells of the logarithmic growth phase of the thallus; washing with phosphate buffer solution for 1-2 times; respectively mixing 15-25% of bacilli, 10-19% of gordonia, 13-27% of pseudomonas putida and 35-45% of sphingomonas YT methyl orange according to the volume percentage to obtain thalli B for heterotrophic nitrification-aerobic denitrification bacteria;
3) according to the volume percentage, 10 percent of the thallus A obtained in the step 1) and 90 percent of the mixed thallus B obtained in the step 2) are jointly inoculated into the water body to be treated for denitrification treatment, and the volume ratio of the mixed thallus to the water body to be treated is 1: 100.
in the application, in the step 1) and the step 2), the nutrient solution mainly comprises the following components: NaCl 10.0g/L, peptone 10.0g/L, yeast powder 5.0g/L, and water in balance, and the pH is natural.
In the above application, in step 2), the proliferation medium mainly comprises: 0.382g/L of ammonium chloride, 1.5g/L of potassium dihydrogen phosphate, 4.2g of disodium hydrogen phosphate, 0.1g of magnesium sulfate, 1g of glucose and the balance of water, and the pH value is natural.
In the above application, the phosphate buffer solution comprises the following components: 9.0g/L of sodium chloride, 0.3g/L of potassium chloride, 1.2g/L of dipotassium phosphate and 0.3g/L of monopotassium phosphate, and the balance of water, wherein the pH value is natural.
In the application, in the step 1) and the step 2), the centrifugal treatment refers to centrifugation at 8000rpm for 5-10 min.
In the application, the water body to be treated is nitrogen-containing wastewater containing ammonia nitrogen and nitrate nitrogen, and is further suitable for fermentation pharmaceutical wastewater containing ammonia nitrogen, nitrate nitrogen and antibiotics.
In the application, 1g/L glucose is additionally added as a carbon source in the treatment process of the nitrogen-containing wastewater.
The invention has the following beneficial effects:
1) the biological nano-selenium reinforced composite strain has the advantages that the biological nano-selenium reaction condition is mild, the prepared nano-selenium has high biological activity, small size, uniform particle size, strong stability and environmental friendliness, and can be adsorbed to microorganisms, and the nano-selenium can act with cytochrome c of mitochondria of the microorganisms to change the oxidation-reduction potential of the cytochrome c and enhance the electron transfer capability, so that the composite strain can generate a synergistic effect to jointly promote heterotrophic nitrification-aerobic denitrification of wastewater. Under the condition of the range value of the embodiment of the invention, the composite bacterial strain prepared by the invention is compared with a single microorganism experiment and a single nano selenium experiment under the same experiment condition, and the composite bacterial agent can be verified to be capable of generating a synergistic effect to jointly promote the heterotrophic nitrification-aerobic denitrification of the wastewater.
2) The composite bacterial strain provided by the invention can grow by taking ammonia nitrogen and nitrate as nitrogen sources respectively, and can synchronously remove the ammonia nitrogen and the nitrate by taking glucose as a carbon source under an aerobic condition. The method is simple and convenient to use, the prepared composite bacterial strain can be directly put into the polluted water body, the in-situ remediation of the polluted water body is realized, the loss of microorganisms is effectively avoided, no secondary pollution is caused, the problem that aerobic nitrification and anaerobic denitrification need to be operated independently in the traditional biological wastewater treatment is solved, and the method has better economic benefit. In addition, the compound bacterial strains belong to heterotrophic bacteria, have high growth rate and can overcome the defect of slow growth of autotrophic nitrifying bacteria.
Drawings
FIG. 1 is a growth characteristic diagram of a composite strain with ammonia nitrogen as a unique nitrogen source;
FIG. 2 is a growth characteristic diagram of biological nano selenium reinforced composite bacterial strain with ammonia nitrogen as the only nitrogen source;
FIG. 3 is a diagram of denitrification performance of a composite strain with ammonia nitrogen as a unique nitrogen source;
FIG. 4 is a denitrification performance diagram of biological nano-selenium enhanced composite bacterial strain with ammonia nitrogen as a unique nitrogen source;
FIG. 5 is a diagram of the growth characteristics of the complex strain with nitrate nitrogen as the only nitrogen source;
FIG. 6 is a growth characteristic diagram of biological nano selenium reinforced composite bacterial strain with nitrate nitrogen as the only nitrogen source;
FIG. 7 is a diagram of denitrification performance of a composite strain with nitrate nitrogen as a unique nitrogen source;
FIG. 8 is a denitrification performance chart of the biological nano-selenium enhanced composite strain with nitrate nitrogen as the only nitrogen source;
FIG. 9 shows the removal of NH from biological nano-selenium enhanced composite bacterial strain 4 + -N energy diagram of actual sewage.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described below with reference to the following embodiments and the accompanying drawings. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The purchasing company of the bacillus megaterium, the sphingomonas YT methyl orange, the pseudomonas putida, the bacilli and the gordonia in the invention is Guangzhou city, Kyowa district, science city, Tokya mountain No. 1, and Guangzhou city, microbial research institute, Inc.
The methods for measuring ammonia nitrogen, nitrate nitrogen and nitrite nitrogen referred to in the following examples are as follows:
ammonia nitrogen is measured by adopting a spectrophotometry method for measuring a nano reagent (GB/T7479-;
measuring nitrite nitrogen by adopting a naphthyl ethylenediamine hydrochloride spectrophotometry (GB/T7493-1987);
the nitrate nitrogen is measured by an ultraviolet spectrophotometry (HJ/T346-;
COD is determined by adopting a rapid closed digestion method (HJ 828 + 2017);
TN was determined by UV spectrophotometry (HJ 636-.
Example 1: biological nano selenium reinforced composite bacterial strain culture
Heterotrophic nitrification culture medium: the solution contained 4.2g Na per liter 2 HPO 4 ，1.5g KH 2 PO 4 ，0.1g MgSO 4 ·7H 2 O, 1g glucose, 0.382g NH 4 Cl。
Denitrification medium: the solution contained 4.2g Na per liter 2 HPO 4 ，1.5g KH 2 PO 4 ，0.1g MgSO 4 ·7H 2 O, 1g glucose, 0.72g NaNO 3 。
LB culture medium: the solution contained 10g tryptone, 5g yeast extract, 10g NaCl per liter. The liquid culture medium and PBS are both prepared by deionized water, and are sterilized by high-pressure moist heat at 121 ℃ for 20min before use.
1) B, synthesizing biological nano selenium by using the bacillus megaterium: 100mL of LB medium was used to inoculate Bacillus megaterium which was incubated at 30 ℃ and 150rpm on a shaker for 24h to log phase. 10mL of the above bacterial suspension was added to 90mL of LB medium and 2.2mL of Na medium containing 1g/L of Na was added 2 SeO 3 And (3) after the concentration of Se is finally 20mg/L, tying the breathable sealing film, putting the mother liquor into a shaking table at 30 ℃ and 150rpm, and continuously culturing for 24 hours until the color of the culture medium is changed from colorless to red, and the sodium selenite in the culture medium is completely reduced and is regarded as the completion of culture, and recording as bacterial liquid A.
2) The cultivation of the biological nano selenium reinforced composite bacterial strain: respectively transferring the mixed thallus of pseudomonas putida, bacilliform bacteria, gordonia and sphingosine monad YT methyl orange into a 100mLLB culture medium, culturing for 48h to logarithmic phase under the conditions of 33 ℃ and 150rpm, centrifuging for 5min at 8000r/min, collecting the thallus, washing for 1-2 times by using phosphate buffer solution, resuspending the thallus, respectively taking 20% of bacilliform bacteria, 20% of gordonia, 20% of pseudomonas putida and 40% of sphingosine monad YT methyl orange according to volume percentage, and mixing and marking as bacterial liquid B. According to the volume percentage, 10 percent of bacillus megaterium bacterial liquid A for synthesizing the biological nano-selenium is mixed with 90 percent of the bacterial liquid B, and then the biological nano-selenium reinforced composite bacterial strain is obtained.
3) Culturing a composite strain of the non-synthesized biological nano selenium: 100mL of LB medium was used to inoculate Bacillus megaterium which was incubated in a shaker at 30 ℃ and 150rpm for 24h to log phase. And mixing 10% of the logarithmic phase bacillus megaterium bacterial liquid with 90% of the bacterial liquid B according to the volume percentage to obtain the culture of the composite bacterial strain of the non-synthesized biological nano-selenium.
Example 2: application of biological nano-selenium reinforced composite strain to ammonia nitrogen-containing wastewater
The biological nano-selenium enhanced composite strain obtained in the example 1 and the composite strain of the non-synthesized biological nano-selenium are respectively taken and inoculated into LB liquid culture medium according to the volume ratio of 1 percent, cultured in a shaker at 30 ℃ and 150rpm for 24 hours, centrifuged at 8000r/min for 5min, thallus is collected, phosphate buffer solution of PBS is used for suspending the thallus, the thallus is inoculated into a conical flask filled with 150mL heterotrophic nitrification culture medium according to the inoculation amount of 10 percent (volume ratio), and the conical flask is placed in a shaker at 30 ℃ and 150 rpm. Sampling at regular intervals, and measuring the Optical Density (OD) of the bacterial liquid 600 ) Ammonia Nitrogen (NH) 4 + -N), nitrous Nitrogen (NO) 2 - -N) and nitrate Nitrogen (NO) 3 - -N) concentration. According to OD 600 And (3) drawing a growth curve of the thalli, wherein as shown in the graphs 1 and 2, the thalli enters a logarithmic growth phase at 12 hours and enters a stationary phase at 43 hours, the maximum OD600 value of the biological nano-selenium reinforced composite strain can reach 1.001, and the maximum OD600 value of the composite strain of the non-synthesized biological nano-selenium can reach 0.980. As shown in figure 4, compared with the compound strain of non-synthesized biological nano-selenium in figure 7, after 60 hours, the removal rate of the bacterial strain YT methyl orange on ammonia nitrogen is higher and can reach 74.5%, nitrate nitrogen is accumulated in a small amount (8.43mg/L), nitrite nitrogen is not accumulated obviously, and the bacterial strain YT methyl orange has stronger ammonia nitrogen removal capability. Compared with the conventional nitrifying bacteria, the strain can complete two processes of nitrification and denitrification, realize the removal of ammonia nitrogen, has no obvious accumulation of nitrate nitrogen and nitrite state, and improves the removal rate of total nitrogen.
Example 3: application of biological nano-selenium reinforced composite bacterial strain to nitrate-containing wastewater
The biological nano-selenium enhanced composite strain obtained in the example 1 and the composite strain of the non-synthesized biological nano-selenium are respectively taken and inoculated into LB liquid culture medium according to the volume ratio of 1 percent, cultured in a shaking table at 30 ℃ and 150rpm for 24 hours, centrifuged at 8000r/min for 5min, the thalli are collected, the thalli are resuspended by PBS phosphate buffer solution, inoculated into a conical flask filled with 150mL of aerobic denitrification culture medium according to the inoculation amount of 10 percent (volume ratio), and placed into the shaking table at 30 ℃ and 150 rpm. Sampling at regular intervals, and measuring the Optical Density (OD) of the bacterial liquid 600 ) Ammonia Nitrogen (NH) 4 + -N), nitrous Nitrogen (NO) 2 - -N) and nitrate Nitrogen (NO) 3 - -N) concentration. According to OD 600 The growth curve of the strain is plotted, as shown in FIGS. 5 and 6, the two composite strains enter logarithmic growth phase at 6 hours, and enter plateau phase at 43 hours, and the biological nano-selenium strengthens the OD of the composite strain 600 The highest value can reach 1.225, and the OD of the composite strain of the non-synthesized biological nano-selenium 600 The value can reach 1.172 at most. As shown in FIG. 8, compared with the fig. 7, the biological nano-selenium reinforced composite strain has a remarkable effect of degrading nitrate nitrogen after 60 hours, the removal rate is higher and can reach 67.11%, the highest accumulation amount of nitrite nitrogen is 16.78mg/L, and finally the maximum accumulation amount of nitrite nitrogen is reduced to 6.94 mg/L.
Example 4: application of biological nano-selenium reinforced composite strain to actual fermentation pharmaceutical wastewater (containing ammonia nitrogen and nitrate nitrogen)
250mL of fermentation pharmaceutical wastewater is taken to be put in a 1L container, the initial ammonia nitrogen of the wastewater is 172.4mg/L, and the COD is 490.8 mg/L. 10mL of the biological nano-selenium-enriched composite strain which is subjected to the expansion culture from the heterotrophic nitrification culture medium in the example 2 to the logarithmic phase is added, and the biological nano-selenium-enriched composite strain is placed at room temperature, and dissolved oxygen is kept above 2ppm by using an aeration device. Sampling at regular time, centrifuging to remove precipitate, and detecting ammonia nitrogen and nitrite state in supernatantNitrogen, nitrate nitrogen and COD concentrations. As shown in figure 9, the biological nano-selenium reinforced composite strain can be well adapted to actual fermentation pharmaceutical wastewater, after 60 hours, the ammonia nitrogen removal rate can reach 72.9%, the COD removal rate can also reach 73.34%, nitrite nitrogen is not obviously accumulated in the process, and nitrate nitrogen is slightly accumulated (10.95 mg/L). The waste water produced in the fermentation pharmacy process has the characteristics of high COD concentration, high nitrogen load, low C/N ratio and the like, and the nitrogen in the waste water mainly exists in the forms of organic nitrogen and ammonia nitrogen, namely BOD 5 the/N is generally between 1 and 4 and is lower than the conventional biological treatment requirement, the sewage often contains residual antibiotics, the bacteriostatic property of the residual antibiotics causes challenges to the traditional biological sewage treatment process, the composite strain has higher antibiotic resistance, the composite strain still has the effect of efficiently removing ammonia nitrogen and total nitrogen when being applied to the sewage treatment, and no obvious accumulation of nitrite nitrogen and nitrate nitrogen is generated compared with other strains applied to the antibiotic sewage.
Claims (10)
1. The biological nano-selenium reinforced composite bacterial strain is characterized in that the composite bacterial agent comprises various combinations of bacillus megaterium, sphingomonas YT methyl orange, pseudomonas putida, bacilliform bacteria and Gordonia for synthesizing biological nano-selenium.
2. The biological nano-selenium enhanced composite microbial inoculum of claim 1, which is characterized in that: the volume ratio of bacillus megaterium, bacilli, gordonia, pseudomonas putida and sphingomonas YT methyl orange is (10-15%): (15-20%): (10-15%): (10-25%): (30-45%) and the viable count of each strain is greater than 4.5 hundred million/g.
3. The biological nano selenium reinforced composite strain applied to total nitrogen treatment of a water body as claimed in claim 1 or 2, wherein the treatment method comprises the following steps:
1) adding Bacillus megaterium solution into nutrient solution, and adding 2.2mL of Na containing 1g/L 2 SeO 3 The inoculation amount of the mother liquor is 5-10% of the volume percentage of the nutrient solution, and the culture temperature is 30-35 DEG CCulturing at the rotation speed of 120-150rpm for 24-48h, and centrifuging to obtain cells in the logarithmic growth phase of the thalli; washing with phosphate buffer solution for 1-2 times to obtain thallus A for synthesizing biological nano-selenium;
2) transferring mixed thallus of Pseudomonas putida, Bacillus, Gordonia and Sphingomonas YT methyl orange into nutrient solution, culturing at 30-35 deg.C and 150rpm for 1-3 days, inoculating 5-18% volume ratio into a container containing proliferation culture medium, culturing at 35-37 deg.C for 1-3 days, and centrifuging to obtain logarithmic phase cells of the thallus; washing with phosphate buffer solution for 1-2 times; respectively mixing 15-25% of bacilli, 10-19% of gordonia, 13-27% of pseudomonas putida and 35-45% of sphingomonas YT methyl orange according to the volume percentage to obtain thalli B for heterotrophic nitrification-aerobic denitrification bacteria;
3) according to the volume percentage, 10 percent of the thallus A obtained in the step 1) and 90 percent of the mixed thallus B obtained in the step 2) are jointly inoculated into the water body to be treated for denitrification treatment, and the volume ratio of the mixed thallus to the water body to be treated is 1: 100.
4. the use according to claim 3, wherein in step 1) and step 2), the nutrient solution comprises the following main components: NaCl 10.0g/L, peptone 10.0g/L, yeast powder 5.0g/L, and water in balance, and the pH is natural.
5. The use according to claim 3, wherein in step 2), the proliferation medium comprises the following main components: 0.382g/L of ammonium chloride, 1.5g/L of potassium dihydrogen phosphate, 4.2g of disodium hydrogen phosphate, 0.1g of magnesium sulfate, 1g of glucose and the balance of water, and the pH value is natural.
6. The use according to claim 3, wherein the phosphate buffer comprises the following components: 9.0g/L of sodium chloride, 0.3g/L of potassium chloride, 1.2g/L of dipotassium phosphate and 0.3g/L of monopotassium phosphate, and the balance of water, wherein the pH value is natural.
7. The use according to claim 3, wherein in step 1) and step 2), the centrifugation is performed at 8000rpm for 5-10 min.
8. The application of the method as claimed in claim 3, wherein the water body to be treated is nitrogen-containing wastewater containing ammonia nitrogen and nitrate nitrogen.
9. The application of the method as claimed in claim 3, wherein the water body to be treated is fermentation pharmaceutical wastewater containing ammonia nitrogen, nitrate nitrogen and antibiotics.
10. Use according to claim 3, characterized in that: in the process of treating the nitrogen-containing wastewater, 1g/L glucose is required to be added as a carbon source.
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| CN115612647A (en) * | 2022-10-20 | 2023-01-17 | 淮阴工学院 | Preparation method of selenium-rich biological floccules |
| CN115812895A (en) * | 2022-12-14 | 2023-03-21 | 广东省科学院生物与医学工程研究所 | Application of biological nano zero-valent palladium material with high photothermal effect in preparation of heating product |
| CN115812895B (en) * | 2022-12-14 | 2024-02-02 | 广东省科学院生物与医学工程研究所 | Application of biological nano zero-valent palladium material with high photo-thermal effect in preparation of heating product |
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