CN107337283B - Method for rapidly culturing pig breeding sewage activated sludge - Google Patents
Method for rapidly culturing pig breeding sewage activated sludge Download PDFInfo
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- CN107337283B CN107337283B CN201710488602.XA CN201710488602A CN107337283B CN 107337283 B CN107337283 B CN 107337283B CN 201710488602 A CN201710488602 A CN 201710488602A CN 107337283 B CN107337283 B CN 107337283B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/38—Gas flow rate
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/44—Time
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
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- Activated Sludge Processes (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for quickly culturing activated sludge in pig breeding sewage, which comprises the steps of preparing a microbial inoculum, diluting the sewage, adding nutrient substances, inoculating the microbial inoculum and carrying out aeration treatment. The beneficial effects are that: the invention has the advantages of simple operation, less required device amount, low cost, no pollution, high efficiency, easy realization and the like; the method comprises the steps of treating the pig breeding sewage by using enterobacter cloacae with a functional microorganism preservation number of CCTCC NO: M2017219 and pseudomonas stutzeri with a preservation number of CCTCC NO: M2017218, removing heavy metals and antibiotics in the sewage, providing a good environment for the growth and the propagation of beneficial bacteria in the activated sludge, accelerating the culture speed of the activated sludge, and reducing the initial inoculation sludge amount in the traditional activated sludge culture by over 50 percent.
Description
Technical Field
The invention relates to the technical field of sewage treatment, in particular to a method for quickly culturing activated sludge in pig breeding sewage.
Background
According to the reports of related documents, the original national environmental protection administration samples and surveys the pollution conditions of 23 provinces (regions) and large-scale livestock and poultry breeding in cities in China, and the national livestock and poultry manure and sewage yield exceeds 200 hundred million tons, which is 2.4 times of the industrial solid waste yield, and the Chemical Oxygen Demand (COD) of the livestock and poultry manure is far more than the sum of the discharge amount of industrial wastewater and domestic sewage COD in China. The livestock and poultry breeding pollution becomes the third pollution source following industrial pollution and living pollution, and becomes one of the main causes of agricultural pollution in China. High-concentration sewage is discharged into rivers and lakes to cause continuous deterioration of surface water and underground water quality, so that the environment is seriously polluted, the body health of people is directly harmed, and the development of the breeding industry is also seriously restricted. The treatment of livestock and poultry breeding wastewater has attracted the attention of the owners of the farms and the relevant departments at present, and a series of prevention and treatment measures and economic and efficient treatment technologies are adopted and are not slow enough. With the gradual update of the national sewage discharge standard, the problem of how to reach the standard discharge of the high-concentration aquaculture wastewater is more prominent.
The activated sludge process is the most widely used wastewater treatment process at present, has the advantages of simplicity, high efficiency and the like, but also has the problems of large occupied area, more excess sludge, high cost, easy sludge bulking and the like. In recent years, the aerobic granular sludge technology, as a new wastewater treatment process developed on the basis of the activated sludge process, can better overcome the problems in the activated sludge process, and thus has attracted extensive attention and attention at home and abroad. The aerobic granular sludge is a dense biopolymer which is compact in structure and regular in shape and is finally formed by microorganisms through a series of complex self-fixing processes under the aerobic environment condition. Compared with the conventional activated sludge process, the aerobic granular sludge process has the advantages of small occupied area, low excess sludge yield, low construction and operation cost and the like, and shows good development and application prospects. However, the formation of aerobic granular sludge tends to be slow and the process conditions are more stringent.
The prior art, such as the Chinese patent with the publication number of CN101767873B, discloses a method for rapidly culturing anaerobic hydrogen-producing granular sludge. The method is characterized in that residual activated sludge which is cultured and pretreated by 60-100 mg/L of cationic polyacrylamide is added into a reactor, the initial sludge concentration in the reactor is 12-25g/L, under the condition of medium temperature (34-35 ℃), sugar-containing wastewater is used as a substrate, the biochemical oxygen demand (COD) of inlet water is 4000mg/L, the pH value is 7.5-8.5, the total water retention time of the reactor is gradually reduced to 3.5h from the initial 15h, and the organic volume load is 6-10 kgCOD/m3D gradually increases to 30kgCOD/m3D, over 7d to 15d, anaerobic hydrogen-producing granular sludge which takes clostridium as a main body and the total volume of sludge with a small amount of filamentous bacteria and cocci coexisting accounts for 25 to 40 percent of the total volume of the reactor can be formed. The reactor filled with the granular sludge can continuously produce fermentation gas with the hydrogen content of 44-56 percentThe gas yield is 10-16 m3/m3 Reactor with a reactor shellD, the hydrogen production efficiency can reach 3.1mol H2Per mol of sucrose. The method has more complex treatment steps, and the culture speed of the activated sludge needs to be improved.
Disclosure of Invention
The invention aims to provide a method for quickly culturing the activated sludge in the pig breeding sewage, which is simple to operate, low in initial sludge inoculation amount and short in culture period.
Aiming at the problems mentioned in the background technology, the invention adopts the technical scheme that: classification of AOZ-1 is named Enterobacter AOZ-1 (Enterobactersp, AOZ-1) with a preservation number of CCTCC NO of M2017219, and is preserved in the China Center for Type Culture Collection (CCTCC) in 2017, 4 and 28 months. The classification of ADH-1 is named as Pseudomonas ADH-1 (Pseudomonassp, ADH-1) with the preservation number of CCTCC NO: M2017218, and is preserved in the China Center for Type Culture Collection (CCTCC) in 2017, 4 and 28 months. The above preservation center is located at Wuhan university in Wuhan, China.
A method for quickly culturing the sewage generated by culturing live pig includes preparing bacterial agent, diluting sewage, adding nutrients, inoculating bacterial agent and aerating. The method has the advantages of simple operation, low cost, no pollution, high efficiency, easy realization and the like.
The step of preparing the microbial inoculum comprises the steps of mixing functional microorganisms enterobacter cloacae and pseudomonas stutzeri into the microbial inoculum according to the weight ratio of 1: 1-1: 2, wherein the enterobacter cloacae has a preservation number of CCTCC NO: M2017219; pseudomonas stutzeri with the preservation number of CCTCC NO: M2017218. The microbial inoculum has adsorption activity on heavy metals and antibiotics, so that the content of the heavy metals and the antibiotics in the culture sewage can be reduced, the growth of beneficial microorganisms in activated sludge is promoted, and the culture period of the traditional aerobic sludge is greatly shortened; by adding functional microorganisms, the initial inoculation sludge amount in the traditional activated sludge culture can be reduced by more than 50%. If the microbial inoculum is changed into enterobacter cloacae and photosynthetic bacteria, the initial inoculation amount of the traditional activated sludge can be reduced by only 6-10%.
The step of diluting the sewage is to mix the live pig breeding sewage and the deep well water according to the weight ratio of 1: 5-1: 10. The culture sewage has high concentration and is not suitable for the growth of functional microorganisms.
The step of adding the nutrient substances is to add 0.7-1.5% of nutrient substances in mass proportion into the diluted sewage, wherein the nutrient substances are one or more of starch slurry, canteen rice swill, noodle soup, urea, sulfur ammonia and ammonia water. The nutrient substances provide a carbon source and a nitrogen source for the functional microorganisms and promote the growth and the propagation of the functional microorganisms, thereby accelerating the reduction of the contents of heavy metals and antibiotics in the sewage.
Inoculating a mixed microbial inoculum with the volume ratio of 0.5-1% in a sewage treatment biochemical treatment tank, and aerating for 12-24 hours under aerobic conditions; the volume ratio of aerobic aeration quantity to water inflow quantity is controlled to be 10: 1-20: 1. The functional microorganism is aerobic bacteria, and needs sufficient oxygen in the growth and reproduction process. In the aeration process, a large amount of functional microorganisms are propagated, heavy metals and antibiotics in the culture sewage are completely adsorbed, and a good environment is provided for the growth and propagation of beneficial bacteria in the activated sludge.
The aeration treatment step is to add aerobic activated sludge with the mass proportion of 1-5% into the culture sewage treated by the functional microorganisms, and carry out aeration culture until the sludge amount reaches more than 6000 mg/L. Beneficial bacteria in the aerobic activated sludge are quickly proliferated to form aerobic activated sludge particles, so that the problem of slow formation of the conventional aerobic activated sludge particles is solved.
Compared with the prior art, the invention has the advantages that: 1) the invention has the advantages of simple operation, less required device amount, low cost, no pollution, high efficiency, easy realization and the like; 2) the method comprises the steps of treating the pig breeding sewage by using enterobacter cloacae with a functional microorganism preservation number of CCTCC NO: M2017219 and pseudomonas stutzeri with a preservation number of CCTCC NO: M2017218, removing heavy metals and antibiotics in the sewage, providing a good environment for the growth and the propagation of beneficial bacteria in activated sludge, accelerating the culture speed of the activated sludge, and reducing the initial inoculation sludge amount in the traditional activated sludge culture by more than 50%.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
The scheme of the invention is further illustrated by the following examples:
example 1:
a method for quickly culturing the sewage generated by culturing live pig includes preparing bacterial agent, diluting sewage, adding nutrients, inoculating bacterial agent and aerating. The method has the advantages of simple operation, low cost, no pollution, high efficiency, easy realization and the like.
The step of preparing the microbial inoculum comprises the steps of mixing functional microorganisms enterobacter cloacae and pseudomonas stutzeri into the microbial inoculum according to the weight ratio of 1:1.6, wherein the enterobacter cloacae has a preservation number of CCTCC NO: M2017219; pseudomonas stutzeri with the preservation number of CCTCC NO: M2017218. The microbial inoculum has adsorption activity on heavy metals and antibiotics, so that the content of the heavy metals and the antibiotics in the culture sewage can be reduced, the growth of beneficial microorganisms in activated sludge is promoted, and the culture period of the traditional aerobic sludge is greatly shortened; by adding functional microorganisms, the initial inoculation sludge amount in the traditional activated sludge culture can be reduced by more than 50%. If the microbial inoculum is changed into enterobacter cloacae and photosynthetic bacteria, the initial inoculation amount of the traditional activated sludge can be reduced by only 6-10%.
The step of diluting the sewage is to mix the live pig breeding sewage and the deep well water according to the weight ratio of 1: 6. The culture sewage has high concentration and is not suitable for the growth of functional microorganisms.
The step of adding nutrient substances is to add 1.2 percent of nutrient substances in mass proportion into the diluted sewage, wherein the nutrient substances are one or more of starch slurry, canteen rice swill, noodle soup water, urea, sulfur ammonia and ammonia water. The nutrient substances provide a carbon source and a nitrogen source for the functional microorganisms and promote the growth and the propagation of the functional microorganisms, thereby accelerating the reduction of the contents of heavy metals and antibiotics in the sewage.
The step of inoculating the microbial inoculum comprises inoculating a mixed microbial inoculum with the volume ratio of 0.8 percent in a sewage treatment biochemical treatment tank, and aerating for 24 hours under aerobic conditions; the volume ratio of aerobic aeration quantity to water inflow quantity is controlled to be 16: 1. The functional microorganism is aerobic bacteria, and needs sufficient oxygen in the growth and reproduction process. In the aeration process, a large amount of functional microorganisms are propagated, heavy metals and antibiotics in the culture sewage are completely adsorbed, and a good environment is provided for the growth and propagation of beneficial bacteria in the activated sludge.
The aeration treatment step is that aerobic activated sludge with the mass proportion of 4 percent is added into the culture sewage treated by the functional microorganisms, and the aeration culture is carried out until the sludge amount reaches more than 6000 mg/L. Beneficial bacteria in the aerobic activated sludge are quickly proliferated to form aerobic activated sludge particles, so that the problem of slow formation of the conventional aerobic activated sludge particles is solved.
Example 2:
a method for quickly culturing activated sludge in pig breeding sewage comprises the following steps:
1) preparing a microbial inoculum: mixing functional microorganisms enterobacter cloacae and pseudomonas stutzeri according to the weight ratio of 1:2 to obtain a microbial agent. Wherein, the preservation number of the Enterobacter cloacae is CCTCC NO: M2017219; pseudomonas stutzeri with a preservation number of CCTCC NO of M2017218;
2) diluting sewage: mixing the live pig breeding sewage and deep well water uniformly in an aeration tank according to the weight ratio of 1: 8;
3) adding nutrient substances: adding 1% by mass of a starch slurry and urea into the diluted sewage;
4) inoculating a microbial inoculum: inoculating a mixed microbial inoculum with the volume ratio of 0.8% in a sewage treatment biochemical treatment tank, and aerating for 24 hours under an aerobic condition; controlling the volume ratio of aerobic aeration quantity to water inflow quantity to be 15: 1;
5) aeration treatment: adding aerobic activated sludge with the mass proportion of 3% into the culture sewage treated by the functional microorganisms, and carrying out aeration culture until the sludge amount reaches more than 6000 mg/L.
Example 3:
a method for quickly culturing activated sludge in pig breeding sewage comprises the following steps:
1) preparing a microbial inoculum: mixing functional microorganisms enterobacter cloacae and pseudomonas stutzeri according to the weight ratio of 1:1 to form a microbial inoculum. Wherein, the preservation number of the Enterobacter cloacae is CCTCC NO: M2017219; pseudomonas stutzeri with a preservation number of CCTCC NO of M2017218;
2) diluting sewage: mixing the live pig breeding sewage and deep well water uniformly in an aeration tank according to the weight ratio of 1: 10;
3) adding nutrient substances: 1% of canteen rice swill, sulfur ammonia and ammonia water by mass are added into the diluted sewage;
4) inoculating a microbial inoculum: inoculating a mixed microbial inoculum with the volume ratio of 0.8% in a sewage treatment biochemical treatment tank, and aerating for 24 hours under an aerobic condition; controlling the volume ratio of aerobic aeration quantity to water inflow quantity to be 20: 1;
5) aeration treatment: adding aerobic activated sludge with the mass proportion of 4% into the culture sewage treated by the functional microorganisms, and carrying out aeration culture until the sludge amount reaches more than 6000 mg/L.
Conventional operations in the operation steps of the present invention are well known to those skilled in the art and will not be described herein.
The embodiments described above are intended to illustrate the technical solutions of the present invention in detail, and it should be understood that the above-mentioned embodiments are only specific embodiments of the present invention, and are not intended to limit the present invention, and any modification, supplement or similar substitution made within the scope of the principles of the present invention should be included in the protection scope of the present invention.
Claims (5)
1. A method for the active sludge rapid culture of the pig breeding sewage comprises the steps of preparing a microbial inoculum, diluting the sewage, adding nutrient substances, inoculating the microbial inoculum and carrying out aeration treatment, and is characterized in that: the prepared microbial inoculum is prepared by mixing enterobacter and pseudomonas;
the enterobacteria are enterobacter cloacae, and the preservation number is CCTCC NO: M2017219;
the pseudomonas is pseudomonas stutzeri with the preservation number of CCTCC NO of M2017218;
the aeration treatment step is to add aerobic activated sludge with the mass ratio of 1-5% into the culture sewage treated by the functional microorganisms, and carry out aeration culture until the sludge amount reaches more than 6000 mg/L.
2. The method for the rapid culture of the activated sludge in the pig breeding sewage according to the claim 1, which is characterized in that: the enterobacteria and the pseudomonas are mixed according to the weight ratio of 1: 1-1: 2.
3. The method for the rapid culture of the activated sludge in the pig breeding sewage according to the claim 1, which is characterized in that: the step of diluting the sewage is to mix the live pig breeding sewage and the deep well water according to the weight ratio of 1: 5-1: 10.
4. The method for the rapid culture of the activated sludge in the pig breeding sewage according to the claim 1, which is characterized in that: the step of adding the nutrient substances is to add 0.7-1.5% of nutrient substances in mass proportion into the diluted sewage, wherein the nutrient substances are one or more of starch slurry, canteen rice swill, noodle soup, urea, sulfur ammonia and ammonia water.
5. The method for the rapid culture of the activated sludge in the pig breeding sewage according to the claim 1, which is characterized in that: the step of inoculating the microbial inoculum comprises inoculating a mixed microbial inoculum with the volume ratio of 0.5-1% in a sewage treatment biochemical treatment tank, and aerating for 12-24 hours under aerobic conditions; the volume ratio of aerobic aeration quantity to water inflow quantity is controlled to be 10: 1-20: 1.
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| CN108823114A (en) * | 2018-05-02 | 2018-11-16 | 中国石油大学(华东) | A kind of cultural method of rubbish deodorization biological enzyme and its application |
| CN108658220A (en) * | 2018-05-14 | 2018-10-16 | 中国科学院城市环境研究所 | Photosynthetic organism pelletizing system for Pollution from livestock and poultry recycling treatment |
| CN111137972B (en) * | 2020-02-26 | 2022-04-19 | 北京联合大学 | Method for quickly generating activated sludge for wastewater treatment |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1962490A (en) * | 2006-12-19 | 2007-05-16 | 北京三泰恒生科技发展有限公司 | High efficient microbe synergistic process for sewage treatment factory |
| CN103382057A (en) * | 2013-07-28 | 2013-11-06 | 华盛江泉集团有限公司 | Method for treating cultivation wastewater |
| CN106497809A (en) * | 2015-09-07 | 2017-03-15 | 粮华生物科技（北京）有限公司 | A kind of enterobacter cloacae, the microbial inoculum containing the bacterium and its application and the method for passivation hydrargyrum |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1962490A (en) * | 2006-12-19 | 2007-05-16 | 北京三泰恒生科技发展有限公司 | High efficient microbe synergistic process for sewage treatment factory |
| CN103382057A (en) * | 2013-07-28 | 2013-11-06 | 华盛江泉集团有限公司 | Method for treating cultivation wastewater |
| CN106497809A (en) * | 2015-09-07 | 2017-03-15 | 粮华生物科技（北京）有限公司 | A kind of enterobacter cloacae, the microbial inoculum containing the bacterium and its application and the method for passivation hydrargyrum |
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