CN107337283B - Method for rapidly culturing pig breeding sewage activated sludge - Google Patents

Abstract

The invention discloses a method for quickly culturing activated sludge in pig breeding sewage, which comprises the steps of preparing a microbial inoculum, diluting the sewage, adding nutrient substances, inoculating the microbial inoculum and carrying out aeration treatment. The beneficial effects are that: the invention has the advantages of simple operation, less required device amount, low cost, no pollution, high efficiency, easy realization and the like; the method comprises the steps of treating the pig breeding sewage by using enterobacter cloacae with a functional microorganism preservation number of CCTCC NO: M2017219 and pseudomonas stutzeri with a preservation number of CCTCC NO: M2017218, removing heavy metals and antibiotics in the sewage, providing a good environment for the growth and the propagation of beneficial bacteria in the activated sludge, accelerating the culture speed of the activated sludge, and reducing the initial inoculation sludge amount in the traditional activated sludge culture by over 50 percent.

Description

A kind of method for rapid cultivation of activated sludge in pig breeding sewage

technical field

The invention relates to the technical field of sewage treatment, in particular to a method for rapid cultivation of activated sludge from pig breeding sewage.

Background technique

According to relevant literature reports, the former State Environmental Protection Administration once conducted a sampling survey on the pollution status of large-scale livestock and poultry breeding industries in 23 provinces (autonomous regions) and cities across the country. The chemical oxygen demand (COD) of livestock and poultry manure far exceeds the sum of the COD emissions of industrial wastewater and domestic sewage in my country. Livestock and poultry breeding pollution has become the third largest source of pollution after industrial pollution and domestic pollution, and has become one of the main causes of agricultural pollution in my country. High-concentration sewage is discharged into rivers and lakes, causing continuous deterioration of surface water and groundwater quality, resulting in serious environmental pollution, directly endangering people's health, and severely restricting the development of the aquaculture industry. At present, the treatment of livestock and poultry breeding wastewater has attracted great attention from farm owners and relevant departments. It is imperative to take a series of control measures and select economical and efficient treatment technologies. With the increasingly updated national sewage discharge standards, the issue of how to discharge high-concentration aquaculture wastewater to meet the standards has become more prominent.

Activated sludge method is currently the most widely used wastewater treatment method. It has the advantages of simplicity and high efficiency, but it also has problems such as large floor space, excess sludge, high cost, and easy sludge bulking. In recent years, aerobic granular sludge technology, as a new wastewater treatment process developed on the basis of activated sludge process, can better overcome the above problems in activated sludge process, so it has caused domestic and international problems. extensive attention and attention. Aerobic granular sludge refers to the formation of dense biological aggregates with compact structure and regular shape through a series of complex self-fixation processes under aerobic conditions. Compared with the conventional activated sludge process, the aerobic granular sludge process has many advantages such as small footprint, less excess sludge output, and low construction and operation costs, showing a good development and application prospect. However, the formation of aerobic granular sludge is often relatively slow, and the requirements for process conditions are relatively strict.

The prior art, such as the Chinese invention patent with the authorization announcement number CN101767873B, discloses a method for rapidly culturing anaerobic hydrogen-producing granular sludge. It is characterized by adding the remaining activated sludge that has been cultured and pretreated with cationic polyacrylamide at 60-100 mg/L, so that the initial sludge concentration in the reactor is 12-25 g/L. ℃), using sugary wastewater as substrate, influent biochemical oxygen demand (COD) of 4000 mg/L, pH value of 7.5 to 8.5, the total hydraulic retention time of the reactor gradually decreased from the initial 15h to 3.5h, and the organic volume load Gradually increase from 6～10kgCOD/m ³ .d to more than 30kgCOD/m ³ .d, the total volume of sludge with clostridium as the main body and a small amount of filamentous bacteria and cocci coexist can be formed within 7d～15d, accounting for the total volume of the reactor 25% to 40% of the total volume of anaerobic hydrogen-producing granular sludge. The reactor filled with this kind of granular sludge can continuously produce fermentation gas with a hydrogen content of 44% to 56%, the gas yield is 10 to _16m3 / ^m3reactor.d , and the hydrogen production efficiency can reach _3.1molH2 /mol sucrose . The treatment steps of this method are relatively complicated, and the speed of the activated sludge cultivation needs to be improved.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a method for rapid cultivation of activated sludge from pig breeding sewage with simple operation, low initial sludge inoculation amount and short cultivation period.

In view of the problems mentioned in the background technology, the present invention adopts the technical scheme as follows: the classification of AOZ-1 is named Enterobacter sp. AOZ-1 ( Enterobacter sp. AOZ-1), and the deposit number is CCTCC NO:M 2017219, in 2017 On April 28, it was deposited in the China Collection of Type Cultures (CCTCC). The classification name of ADH-1 is Pseudomonas sp. ADH-1 ( Pseudomonas sp. ADH-1), the deposit number is CCTCC NO: M 2017218, and it was deposited in the China Center for Type Culture Collection (CCTCC) on April 28, 2017 . The above-mentioned collection center is located at Wuhan University, Wuhan, China.

A method for rapidly culturing activated sludge in swine breeding sewage includes preparing bacterial agent, diluting sewage, adding nutrients, inoculating bacterial agent and aeration treatment. The above operation is simple, and has the advantages of low cost, no pollution, high efficiency, and easy realization.

The step of preparing a bacterial agent is to mix functional microorganisms Enterobacter cloacae and Pseudomonas stutzeri in a weight ratio of 1:1 to 1:2 to form a bacterial agent, Enterobacter cloacae, preservation number is CCTCC NO:M 2017219; Pseudomonas stutzeri Monomonas, the deposit number is CCTCCNO:M 2017218. The above bacterial agents have adsorption activity to heavy metals and antibiotics, thereby reducing the content of heavy metals and antibiotics in aquaculture sewage, promoting the growth of beneficial microorganisms in activated sludge, and greatly shortening the culture period of traditional aerobic sludge; through the addition of functional microorganisms, It can reduce the initial amount of sludge inoculated by more than 50% in traditional activated sludge culture. If the bacterial agent is changed to Enterobacter cloacae and photosynthetic bacteria, the initial inoculation amount of traditional activated sludge can only be reduced by 6-10%.

The step of diluting the sewage is to mix the pig breeding sewage and the deep well water in a weight ratio of 1:5 to 1:10. The high concentration of aquaculture sewage is not suitable for the growth of functional microorganisms.

The step of adding nutrients is to add 0.7-1.5% mass ratio of nutrients to the diluted sewage, and the nutrients are one or more of starch slurry, canteen rice swill, noodle soup, urea, sulfur ammonia, and ammonia water. Nutrients provide carbon and nitrogen sources for functional microorganisms to promote their growth and reproduction, thereby accelerating the reduction of heavy metals and antibiotics in sewage.

The step of inoculating the bacterial agent is to inoculate the mixed bacterial agent with a volume ratio of 0.5% to 1% in the sewage treatment biochemical treatment tank, and aerate it for 12 to 24 hours under aerobic conditions; the volume ratio of aerobic aeration and water inflow is controlled by 10: 1~20:1. Functional microorganisms are aerobic bacteria, which require sufficient oxygen in the process of growth and reproduction. During the aeration process, functional microorganisms multiply, and the heavy metals and antibiotics in the aquaculture sewage are completely adsorbed, providing a good environment for the growth and reproduction of beneficial bacteria in the activated sludge.

The aeration treatment step is to add aerobic activated sludge with a mass ratio of 1% to 5% in the aquaculture sewage treated with functional microorganisms, and aeration culture until the sludge amount reaches more than 6000mg/L. The beneficial bacteria in the aerobic activated sludge multiply rapidly to form aerobic activated sludge particles, which solves the problem of slow formation of traditional aerobic activated sludge particles.

Compared with the prior art, the advantages of the present invention are: 1) the present invention is very simple to operate, requires less equipment, has the advantages of low cost, no pollution, high efficiency, easy implementation, etc.; 2) utilizes the functional microorganism preservation number It is Enterobacter cloacae of CCTCC NO: M2017219 and Pseudomonas stutzeri with a preservation number of CCTCC NO: M 2017218 to treat pig breeding sewage, remove heavy metals and antibiotics in sewage, and grow beneficial bacteria in activated sludge Propagation provides a good environment, accelerates the cultivation of activated sludge, and can reduce the initial amount of sludge inoculated by more than 50% in traditional activated sludge cultivation.

specific embodiment

Below by embodiment, the scheme of the present invention is further described:

Embodiment 1:

A method for rapidly culturing activated sludge in swine breeding sewage includes preparing bacterial agent, diluting sewage, adding nutrients, inoculating bacterial agent and aeration treatment. The above operation is simple, and has the advantages of low cost, no pollution, high efficiency, and easy realization.

The step of preparing the microbial inoculum is to mix the functional microorganisms Enterobacter cloacae and Pseudomonas stutzeri in a weight ratio of 1:1.6 to form a microbial inoculum, Enterobacter cloacae, the preservation number is CCTCC NO:M 2017219; Pseudomonas stutzeri, The deposit number is CCTCC NO:M 2017218. The above bacterial agents have adsorption activity to heavy metals and antibiotics, thereby reducing the content of heavy metals and antibiotics in aquaculture sewage, promoting the growth of beneficial microorganisms in activated sludge, and greatly shortening the culture period of traditional aerobic sludge; through the addition of functional microorganisms, It can reduce the initial amount of sludge inoculated by more than 50% in traditional activated sludge culture. If the bacterial agent is changed to Enterobacter cloacae and photosynthetic bacteria, the initial inoculation amount of traditional activated sludge can only be reduced by 6-10%.

The step of diluting sewage is to mix swine breeding sewage and deep well water in a weight ratio of 1:6. The high concentration of aquaculture sewage is not suitable for the growth of functional microorganisms.

The step of adding nutrients is to add 1.2% mass ratio of nutrients to the diluted sewage, and the nutrients are one or more of starch slurry, canteen rice swill, noodle soup, urea, sulfur ammonia, and ammonia water. Nutrients provide carbon and nitrogen sources for functional microorganisms to promote their growth and reproduction, thereby accelerating the reduction of heavy metals and antibiotics in sewage.

The step of inoculating bacterial agent is to inoculate a mixed bacterial agent with a volume ratio of 0.8% in the sewage treatment biochemical treatment tank, and aerate it for 24 hours under aerobic conditions; the volume ratio of aerobic aeration to water inflow is controlled at 16:1. Functional microorganisms are aerobic bacteria, which require sufficient oxygen in the process of growth and reproduction. During the aeration process, functional microorganisms multiply, and the heavy metals and antibiotics in the aquaculture sewage are completely adsorbed, providing a good environment for the growth and reproduction of beneficial bacteria in the activated sludge.

The aeration treatment step is to add aerobic activated sludge with a mass ratio of 4% to the aquaculture sewage treated with functional microorganisms, and aerate and cultivate until the sludge amount reaches more than 6000 mg/L. The beneficial bacteria in the aerobic activated sludge multiply rapidly to form aerobic activated sludge particles, which solves the problem of slow formation of traditional aerobic activated sludge particles.

Example 2:

A method for rapid cultivation of activated sludge in pig breeding sewage, comprising the following steps:

1) Preparation of bacterial agent: Mix functional microorganisms Enterobacter cloacae and Pseudomonas stutzeri in a weight ratio of 1:2 to form a bacterial agent. Wherein, Enterobacter cloacae, preservation number is CCTCC NO:M 2017219; Pseudomonas stutzeri, preservation number is CCTCCNO:M 2017218;

2) Diluted sewage: Mix live pig breeding sewage and deep well water in the aeration tank at a weight ratio of 1:8;

3) Add nutrients: add 1% mass ratio of starch slurry and urea to the diluted sewage;

4) Inoculating bacteria agent: inoculate a mixed bacteria agent with a volume ratio of 0.8% in the sewage treatment biochemical treatment tank, and aerate it for 24 hours under aerobic conditions; the volume ratio of aerobic aeration to water inflow is controlled at 15:1;

5) Aeration treatment: Add aerobic activated sludge with a mass ratio of 3% to the aquaculture sewage treated with functional microorganisms, and aerate and cultivate until the amount of sludge reaches more than 6000mg/L.

Example 3:

A method for rapid cultivation of activated sludge in pig breeding sewage, comprising the following steps:

1) Preparation of bacterial agent: Mix functional microorganisms Enterobacter cloacae and Pseudomonas stutzeri in a weight ratio of 1:1 to form a bacterial agent. Wherein, Enterobacter cloacae, preservation number is CCTCC NO:M 2017219; Pseudomonas stutzeri, preservation number is CCTCCNO:M 2017218;

2) Diluted sewage: Mix the pig breeding sewage and deep well water evenly in the aeration tank at a weight ratio of 1:10;

3) Add nutrients: add 1% mass ratio of canteen rice swill, sulfur ammonia and ammonia water to the diluted sewage;

4) Inoculating bacteria agent: inoculate a mixed bacteria agent with a volume ratio of 0.8% in the sewage treatment biochemical treatment tank, and aerate it for 24 hours under aerobic conditions; the volume ratio of aerobic aeration to water inflow is controlled at 20:1;

5) Aeration treatment: Add aerobic activated sludge with a mass ratio of 4% to the aquaculture sewage treated with functional microorganisms, and aerate and cultivate until the amount of sludge reaches more than 6000mg/L.

Routine operations in the operation steps of the present invention are well known to those skilled in the art and will not be repeated here.

The above embodiments describe the technical solutions of the present invention in detail. It should be understood that the above are only specific embodiments of the present invention and are not intended to limit the present invention. Anything done within the scope of the principles of the present invention Any modifications, additions or substitutions in similar manners, etc., shall be included within the protection scope of the present invention.

Claims (5)

1. a method for rapid cultivation of activated sludge in live pig breeding sewage, comprising preparing a bacterial agent, diluting sewage, adding nutrients, inoculating a bacterial agent and aeration treatment, it is characterized in that: the prepared bacterial agent is Enterobacter Mixed with Pseudomonas; Described Enterobacter is Enterobacter cloacae, and the deposit number is CCTCC NO:M 2017219; Described Pseudomonas is Pseudomonas stutzeri, and the deposit number is CCTCC NO:M 2017218; The aeration treatment step is to add aerobic activated sludge with a mass ratio of 1% to 5% in the aquaculture sewage treated with functional microorganisms, and aerate and cultivate until the sludge amount reaches more than 6000 mg/L. 2. a kind of method for rapid cultivation of activated sludge in pig breeding sewage according to claim 1, is characterized in that: described Enterobacter and Pseudomonas are mixed according to the weight ratio of 1:1～1:2 . 3. a kind of method for fast cultivation of activated sludge of pig breeding sewage according to claim 1, is characterized in that: described dilution sewage step is to press 1:5～1:10 of live pig breeding sewage and deep well water Mix by weight. 4. a kind of method for quick cultivation of activated sludge in pig breeding sewage according to claim 1, is characterized in that: described adding nutrient substance step is to add 0.7～1.5% quality in diluted sewage The proportion of nutrients, the nutrients are one or more of starch slurry, canteen rice swill, noodle soup, urea, sulfur ammonia, and ammonia water. 5. a kind of method for fast cultivation of activated sludge in pig breeding sewage according to claim 1, is characterized in that: described inoculation step is to inoculate the volume ratio in the sewage treatment biochemical treatment tank to be 0.5%～ 1% mixed bacterial agent, aerated for 12~24 hours under aerobic conditions; the volume ratio of aerobic aeration to water inflow is controlled at 10:1~20:1.